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Gene information
General information
TC001418 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451510.1: 109935..111456
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001418
    iBeetle screen iB_00250

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection



    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    delayed (still larva) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001418 RA
      MATPIKDVI LKENAPVTI LFDKHIEFL RDYGKDDNN 
      FEFGMTDYL RVSGMYWGL TALELLDQT HSSPQEEIV 
      TYIKNCQDP ETGGISACL GHDPHLLHT LSGVQILAM 
      YDRLDAIDV EAVVKYVKS LQQPDGSFT GDKWGEVDT 
      RFSFCAVAT LSLLKRLDA VDVDNAVKF VESCMNFDG 
      GFGSRPLSE SHAGLIYCC LGFLSITHR LDLVKRDVL 
      AWWLCERQL PSGGLNGRP EKLPDVCYS WWVLSSLTI 
      LGRLHWISG EKLKKFILA CQDTETGGF ADRPGDVPD 
      PYHTVFGLA ALSLLGQAE IKNVNPTYC MPQHVIDSM 
      QLSPQILRD
    CDS sequence
    • >TC001418 RA
      atggcaaca ccaataaaa gacgtgatt ttgaaagaa 
      aatgcgcca gttacgata cttttcgac aaacacatc 
      gaattcctg cgggactac ggcaaagac gacaacaat 
      ttcgagttc ggtatgacg gactacctc cgcgtttcc 
      ggcatgtac tggggctta acagcgctg gagctcctc 
      gaccagaca cattcctcg cctcaggaa gagatcgtc 
      acttatatc aaaaactgc caagacccg gaaacgggc 
      ggaatcagt gcgtgcttg gggcacgac ccacatttg 
      ctccacact ttgagcggc gttcagatt ttagcaatg 
      tacgaccga ttggacgca attgatgtc gaggctgtt 
      gtgaaatac gtcaagtcg ttgcaacaa cccgacgga 
      agttttacg ggagacaaa tggggcgaa gttgatacg 
      aggttttcg ttttgtgcg gtggccacc ttgtctctg 
      ctcaagaga ctggacgct gttgacgtc gacaacgcc 
      gtgaaattt gtcgagtct tgcatgaat ttcgacgga 
      gggttcggc tcgcggccg ctgtcggag agccacgcg 
      gggttgatt tactgttgt ctggggttt ttgtcaata 
      acccaccgt cttgacttg gtgaagagg gacgttcta 
      gcgtggtgg ctgtgcgag agacagcta ccgtcgggg 
      ggcctcaac ggccggccg gagaagctc cccgacgtt 
      tgctactcg tggtgggtt ttgtcctcc ttgactatt 
      ttggggcga cttcattgg ataagtggc gagaaatta 
      aaaaaattc attctggcg tgtcaagac accgaaact 
      gggggcttc gcagaccgg cctggcgac gtgcctgac 
      ccttatcac actgttttc ggccttgcg gccctatcg 
      ctgctcggc caagccgaa atcaaaaac gtcaatccc 
      acttactgt atgccgcaa cacgttatt gatagcatg 
      caactatcg cctcaaata ttacgtgat taa
    mRNA sequence
    • >TC001418 RA
      tttgactag caatgaggt tttcaaaac gggcggcaa 
      aaattttga actttttca aactgtcag tgttgccaa 
      gccaccgac cccaacacg ccacttgtc atcttgaca 
      gcaaatatt gtggttatg tttgacaat ggcaacacc 
      aataaaaga cgtgatttt gaaagaaaa tgcgccagt 
      tacgatact tttcgacaa acacatcga attcctgcg 
      ggactacgg caaagacga caacaattt cgagttcgg 
      tatgacgga ctacctccg cgtttccgg catgtactg 
      gggcttaac agcgctgga gctcctcga ccagacaca 
      ttcctcgcc tcaggaaga gatcgtcac ttatatcaa 
      aaactgcca agacccgga aacgggcgg aatcagtgc 
      gtgcttggg gcacgaccc acatttgct ccacacttt 
      gagcggcgt tcagatttt agcaatgta cgaccgatt 
      ggacgcaat tgatgtcga ggctgttgt gaaatacgt 
      caagtcgtt gcaacaacc cgacggaag ttttacggg 
      agacaaatg gggcgaagt tgatacgag gttttcgtt 
      ttgtgcggt ggccacctt gtctctgct caagagact 
      ggacgctgt tgacgtcga caacgccgt gaaatttgt 
      cgagtcttg catgaattt cgacggagg gttcggctc 
      gcggccgct gtcggagag ccacgcggg gttgattta 
      ctgttgtct ggggttttt gtcaataac ccaccgtct 
      tgacttggt gaagaggga cgttctagc gtggtggct 
      gtgcgagag acagctacc gtcgggggg cctcaacgg 
      ccggccgga gaagctccc cgacgtttg ctactcgtg 
      gtgggtttt gtcctcctt gactatttt ggggcgact 
      tcattggat aagtggcga gaaattaaa aaaattcat 
      tctggcgtg tcaagacac cgaaactgg gggcttcgc 
      agaccggcc tggcgacgt gcctgaccc ttatcacac 
      tgttttcgg ccttgcggc cctatcgct gctcggcca 
      agccgaaat caaaaacgt caatcccac ttactgtat 
      gccgcaaca cgttattga tagcatgca actatcgcc 
      tcaaatatt acgtgatta aaattgata gatccaacc 
      ttatatgct aattttgat aagttttag cccgcacgt 
      gattttaat gtatattgg tttaatttt ataatgata 
      ataaagccg atttctaag tgatttgtg ttttgtttg 
      gactgcggt ccgcataat tgattttga tgatttagg 
      tcgagcccg gtgtagcca ttttcaaag tgcctgctt 
      tttctccac aactatacg gaaaacgaa cgcgcattt 
      agggcggcc gtgtgtcac cacctgccc caagaagtc 
      ctcaaattg ggggtactt gcccgggaa gccggtccc 
      catgggaga agacacacc gtttgggtg ggtattcaa 
      gtcttactc attatcact gctttcaaa ttaaaacta 
      gagtctcaa t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY