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Gene information
General information
TC001366 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 15098031..15099842
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001366
    iBeetle screen iB_00246

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001366 RA
      MSALRLNKN LLKRCKKFK CFSTAVLQE DEYTATPQY 
      PPILDMSPE KVRERRKEE EYEGIKAVK TVEEKQIKL 
      NMPKYYGFK SYMLYEDYI PYNSLPLIQ HVTRTHLIK 
      NENLPNFYN DLDVTSALN DVKSDIEEA ILIELARHR 
      QTLDLQGDK VEADNRLGF ALSKQINRI ITTRMSRHL 
      PHLKGAQVD LNSRIKSFW LAGGMSPPE NIKRYRRGC 
      SWQKDKENE PTNRVMNYI GSANVSLRT DLPLKHIIS 
      SSDAESPDL SVPFFEFDP RTVGTVANQ HRHIANVPG 
      FWPGDSREF GFLSYHKRG HLISRHYGD ANELKEAVD 
      RQGILASFG WLNAQANFQ GFTTFNDVT YPFVTQTVV 
      TNGQVWSFY VYQLNTIVN HSKFFTENA KRNVCWGTP 
      EVKLFEGVE NDKLVGFND EVLKMLLKF YANVPEVKL 
      GVNLKPFLG KEEKFIADY EDGDKREWL EREYKFLVS 
      NRPRAKLEY EIYAWEKIY KIDHQTRFM DQKKRPFEF 
      KIKPSNRRL DERLPRYIP RALRPELPR HKGRYAKEY 
      FP
    CDS sequence
    • >TC001366 RA
      atgtcggct ttaagactc aacaaaaac cttttaaag 
      cgttgcaaa aagtttaaa tgcttttcg acggctgtt 
      ttgcaagaa gatgagtac acggctacg ccccagtac 
      ccaccaatt ctcgacatg agcccagaa aaggtccga 
      gagcgcaga aaagaagaa gagtatgaa ggaatcaaa 
      gctgtgaag acagtcgag gaaaaacag attaaatta 
      aacatgcct aaatattac ggtttcaaa agctacatg 
      ttgtacgaa gattacatc ccttacaac agtttgccg 
      ttaattcag cacgtcaca aggacacat ttgattaaa 
      aacgaaaat ttgccgaac ttttacaac gatttggac 
      gttacaagt gccctaaac gatgtaaaa tcggacatt 
      gaggaagct attttgatc gaattggcg agacataga 
      caaactctt gatttacaa ggggacaaa gtggaggct 
      gacaaccgc ctcggattt gccttgtcc aaacaaatc 
      aaccgaata atcacaact cgaatgagt cgccacttg 
      ccgcattta aaaggggcc caggttgat ttaaactca 
      agaattaag tcgttttgg ctcgcgggg ggcatgagc 
      cctcctgag aacatcaaa cgatacaga aggggttgc 
      tcatggcaa aaagacaaa gaaaatgag ccaacaaac 
      cgagtcatg aattacatc ggttcagca aacgtatct 
      ttaagaacc gacttgccc ttgaaacac attatttca 
      tcaagcgat gcggaaagt cccgacttg agtgttcca 
      ttttttgaa tttgatccg cgaactgtc ggcactgtg 
      gccaatcag catcgccac atcgccaac gttccaggt 
      ttctggcct ggtgattcc agggagttt ggttttttg 
      tcgtaccac aagcgcggc catttgatt tcgcggcac 
      tacggtgat gctaatgag cttaaggaa gctgttgac 
      cgacagggg attttagcg tcatttggt tggctgaac 
      gcacaagcc aactttcag ggttttacc acatttaat 
      gacgtcacg tatccgttt gtgacgcaa actgtggtc 
      acaaatggg caagtttgg tcgttttat gtctaccaa 
      cttaacact attgttaat cattctaag ttttttacg 
      gaaaatgcg aaacgaaat gtgtgttgg ggaaccccc 
      gaggttaag ttgtttgaa ggggtggag aatgataag 
      ttggttggg tttaatgat gaggttttg aaaatgttg 
      ctgaaattt tatgccaac gtgcctgag gtgaaacta 
      ggcgttaat ttgaagccg tttttgggg aaagaagag 
      aagtttatc gctgattat gaggatggg gataagaga 
      gaatggctt gagagggag tataaattt ttagtatca 
      aataggccg agggctaaa ctcgagtat gagatttac 
      gcttgggaa aaaatttat aaaattgat caccagacg 
      aggtttatg gatcagaag aaaaggcct tttgagttt 
      aaaattaaa ccgtcgaat agaaggctg gatgagcga 
      ttaccgagg tatattccg agggctttg aggcccgag 
      ttgccacga cataaagga cgatacgcc aaggagtat 
      ttcccctaa
    mRNA sequence
    • >TC001366 RA
      aatgattcc taacctcac attgaaaaa tgtcggctt 
      taagactca acaaaaacc ttttaaagc gttgcaaaa 
      agtttaaat gcttttcga cggctgttt tgcaagaag 
      atgagtaca cggctacgc cccagtacc caccaattc 
      tcgacatga gcccagaaa aggtccgag agcgcagaa 
      aagaagaag agtatgaag gaatcaaag ctgtgaaga 
      cagtcgagg aaaaacaga ttaaattaa acatgccta 
      aatattacg gtttcaaaa gctacatgt tgtacgaag 
      attacatcc cttacaaca gtttgccgt taattcagc 
      acgtcacaa ggacacatt tgattaaaa acgaaaatt 
      tgccgaact tttacaacg atttggacg ttacaagtg 
      ccctaaacg atgtaaaat cggacattg aggaagcta 
      ttttgatcg aattggcga gacatagac aaactcttg 
      atttacaag gggacaaag tggaggctg acaaccgcc 
      tcggatttg ccttgtcca aacaaatca accgaataa 
      tcacaactc gaatgagtc gccacttgc cgcatttaa 
      aaggggccc aggttgatt taaactcaa gaattaagt 
      cgttttggc tcgcggggg gcatgagcc ctcctgaga 
      acatcaaac gatacagaa ggggttgct catggcaaa 
      aagacaaag aaaatgagc caacaaacc gagtcatga 
      attacatcg gttcagcaa acgtatctt taagaaccg 
      acttgccct tgaaacaca ttatttcat caagcgatg 
      cggaaagtc ccgacttga gtgttccat tttttgaat 
      ttgatccgc gaactgtcg gcactgtgg ccaatcagc 
      atcgccaca tcgccaacg ttccaggtt tctggcctg 
      gtgattcca gggagtttg gttttttgt cgtaccaca 
      agcgcggcc atttgattt cgcggcact acggtgatg 
      ctaatgagc ttaaggaag ctgttgacc gacagggga 
      ttttagcgt catttggtt ggctgaacg cacaagcca 
      actttcagg gttttacca catttaatg acgtcacgt 
      atccgtttg tgacgcaaa ctgtggtca caaatgggc 
      aagtttggt cgttttatg tctaccaac ttaacacta 
      ttgttaatc attctaagt tttttacgg aaaatgcga 
      aacgaaatg tgtgttggg gaacccccg aggttaagt 
      tgtttgaag gggtggaga atgataagt tggttgggt 
      ttaatgatg aggttttga aaatgttgc tgaaatttt 
      atgccaacg tgcctgagg tgaaactag gcgttaatt 
      tgaagccgt ttttgggga aagaagaga agtttatcg 
      ctgattatg aggatgggg ataagagag aatggcttg 
      agagggagt ataaatttt tagtatcaa ataggccga 
      gggctaaac tcgagtatg agatttacg cttgggaaa 
      aaatttata aaattgatc accagacga ggtttatgg 
      atcagaaga aaaggcctt ttgagttta aaattaaac 
      cgtcgaata gaaggctgg atgagcgat taccgaggt 
      atattccga gggctttga ggcccgagt tgccacgac 
      ataaaggac gatacgcca aggagtatt tcccctaaa 
      acttggcaa atttgtcaa tagattgta atacaaata 
      aaataataa aaaagtaaa acaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY