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Gene information
General information
TC001362 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid12: 6765..11801
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001362
    iBeetle screen iB_00245
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001362 RA
      MGRGGVGST RSEFLAEDS VDDIRYHRN GGCFVTTKR 
      AIAFVLVAV VSLLLVVIL MYYYGPSRK LEALQDDMK 
      DIEELINKT INEEPEENI RLPTNLKPL HYRLRIFPI 
      LDEFSPDNF TYSGEVKII IRCLTKTNK IVLNLEDLE 
      VSEHNVTVS TLKTTILRY ESLDKESDK DQPEMYQKN 
      STHNSSVII VESENNSTD EILIGAEAK SLMIQEVYK 
      EENYKLYIT MKNLLEAGH NYTINIKFS GNITNNLAG 
      FYRTSYKDL SGQRKWLAT TYFQPIFAR RVFPCFDEP 
      NFKSSFEIS IARRTNMTV RSNMPLRET EPIAEKPGW 
      VWDHFEKSL PMPTYLVSF TVCDFHNLH LNSSETGPV 
      INLWAPQSD LPKAKYALE AAQSILIFL EDYLGIKYP 
      LPKIDLLAV PNFARGSMG SWGILSFQK SSILLEEHS 
      RNWELKQHI FIALAHELA HQWFGNLVT MKWWNDLWL 
      NEGIGSFMA EVVQTSLKP RWQSSNDFP VRVMYKSFS 
      LDSLKSTHP IQTDFTKTV QIEQIFDTI IQSKGTSLI 
      RMLNYTLSR GVFRQGLQY YLNSLSYKS TDQDSLWKS 
      LTEIAKNSS VLPDDVTVK SFMESWTKQ KGYPYVTVD 
      RNYDTGEAV ITQQIFIQD NVNNNTLWF IPLSFTTTD 
      DVIHNAWLK NERNTTLNI TSPGNTSWV LFNIDQAGY 
      FRVNYDIHN WNLLIRQLF LNPSEIPVA SRSQLIDDA 
      FELAQMEIL NYSIPLRLT KYLTWKESN YIPWTTALN 
      SLEEIRFII NNYEYTGAF ETYMTHLIR HKFDSLGTR 
      EKPGEPQND KILRWRLTK IACELRYEP CIRWAQKEF 
      YAWMENADP DAKNPIPFE YRSTVQCIG IKTGSLKEW 
      DFLWNRTLE PQISPADLK TAYLSLGCT HDPWLINRY 
      LELSLSGNI TLEYIPYVW QSINHFVGI RTGFHFLRL 
      NWERVYSTY EDVDLVLNS ICQDFLSYL STEIDLQDL 
      TQFYKIHQK DLKSVAPLM QAAVDSIKI RISWRERHL 
      NSVVAWLTN K
    CDS sequence
    • >TC001362 RA
      atgggtcgt ggaggtgtt ggttcaaca aggtccgaa 
      tttttggcc gaagattcg gttgatgat atcagatat 
      cacagaaat gggggatgt tttgtgacc actaaaagg 
      gccatagct ttcgttctg gtagctgtt gtgtccttg 
      cttctggtt gtgatcttg atgtactac tatggacct 
      agtcggaag ttggaagcg ttgcaagac gacatgaaa 
      gatatcgag gaactgatc aacaagaca ataaatgag 
      gagcctgaa gaaaatatc cgactacca acaaaccta 
      aaaccactt cattatcgg cttagaatt tttccgatt 
      ctggacgaa ttttcgcct gataatttc acttactcg 
      ggagaagtg aaaataatc attcggtgt ttaacaaaa 
      accaacaaa atagttcta aatttggaa gatttggaa 
      gttagtgaa cacaacgtg actgtatcg acacttaaa 
      accacaatt ttacgttac gaaagtttg gataaagag 
      agtgataaa gaccagcct gaaatgtac cagaaaaat 
      tcgactcat aacagttcg gtaataatt gtggaaagt 
      gagaataat tcaacggat gaaattctg attggagcc 
      gaagctaag tcgctcatg atccaggaa gtttataaa 
      gaagagaat tataagttg tacatcact atgaaaaat 
      ttgctagaa gctgggcat aattatacg attaatatc 
      aaattttcc gggaatatt actaataat ttggcgggg 
      ttttatcgg acgagttat aaggatttg tcagggcag 
      agaaaatgg ttggcaacg acttatttt caaccaatt 
      tttgcaaga agagtgttt ccttgcttt gatgaacca 
      aattttaaa tcgtccttt gagatcagt attgcaagg 
      aggactaat atgacagtg aggtcgaat atgccactc 
      agggaaacc gagcccatc gctgaaaaa ccgggatgg 
      gtttgggac cactttgaa aagtccctc ccaatgccc 
      acctacttg gtctcattc acagtttgc gacttccac 
      aatttacac ttaaattca tccgagacc gggcctgtt 
      ataaatttg tgggcccct cagagcgac ttacccaaa 
      gccaaatac gcgcttgaa gcagctcag agcatttta 
      atcttcttg gaagactat ctaggcatt aaatatcca 
      ctcccaaaa attgacctt ctggcagtt ccaaatttc 
      gcccgcggt tcaatggga agttggggc attttatcg 
      tttcaaaaa tcctcaatc ctccttgag gaacattcg 
      agaaattgg gagttgaaa cagcacatc ttcattgct 
      ttggcccat gaattggcc caccagtgg tttggtaat 
      ttggtcaca atgaagtgg tggaacgat ttgtggttg 
      aacgaaggc attgggagt tttatggcc gaagttgtg 
      caaactagc ttgaagccc aggtggcaa agcagcaac 
      gattttccc gtgagggtg atgtacaaa agcttcagt 
      ttggacagt ttgaagtca actcatccg attcagacc 
      gatttcacc aaaacggtg caaattgag caaattttc 
      gatacgatt atacaaagc aaaggcacg agtttgatc 
      cggatgttg aattatacg ttatcaagg ggggttttc 
      cgacaagga ctccaatac tatttgaat agtttatcg 
      tacaaatca accgatcaa gactcgctc tggaaatca 
      ctaactgaa atcgctaaa aattcgtcg gttcttccg 
      gacgatgtg acggtgaag agcttcatg gagtcgtgg 
      accaaacaa aaaggatat ccttatgta accgttgac 
      cggaattat gacactgga gaggctgtt attactcag 
      caaattttt attcaagat aatgtcaac aataacact 
      ttgtggttt attccgctg agctttacc acaacggat 
      gatgttatt cacaatgcg tggttgaaa aatgaaaga 
      aatacaact ctgaacatt acgagtccg gggaatact 
      tcttgggtg ctttttaac atcgatcaa gcaggttac 
      tttcgcgta aattacgac attcacaat tggaacctc 
      ctcatccgc caactgttc ctcaaccct tcggaaatc 
      cctgtggca agtcgcagc caactgatc gatgatgct 
      ttcgaatta gcccaaatg gagatcctc aattacagt 
      attccactg cgtctcacc aaatacctg acttggaaa 
      gaatcgaat tacattccc tggaccaca gcactcaac 
      agtttggag gaaatccga ttcattatc aacaattat 
      gaatacact ggagctttt gaaacgtat atgacgcat 
      ctgattaga cacaaattt gattcgttg ggaacgcgt 
      gaaaaaccc ggggaaccc caaaacgac aaaatcctg 
      aggtggagg ttgacgaaa atcgcctgc gaactgaga 
      tacgagccg tgcattcgg tgggcacag aaggagttt 
      tacgcatgg atggaaaat gcagatcct gatgcgaaa 
      aatccgatt ccgtttgag tatcgctcg acggtgcaa 
      tgcattgga attaaaaca gggagtttg aaggagtgg 
      gatttccta tggaatcgc actcttgag cctcaaata 
      tcgccagct gatttgaaa actgcatat ttgagtctg 
      ggttgtacg cacgatcct tggctcatt aacaggtac 
      ttggagttg tctttaagt ggcaatatt acgctggaa 
      tacatccct tatgtctgg cagtcaata aatcacttt 
      gttggaatt cggacaggt ttccatttt ttgaggttg 
      aactgggaa cgcgtttac agtacttat gaggatgtt 
      gatttggtg cttaattca atttgtcaa gactttctc 
      tcctatttg tccacagag attgattta caggatttg 
      actcaattc tataaaata catcagaag gatttgaaa 
      tcggtggca ccgctaatg caagctgca gttgattca 
      atcaaaatt cgaatcagt tggagggaa aggcacttg 
      aattcagtc gtcgcctgg ctgaccaat aagtaa
    mRNA sequence
    • >TC001362 RA
      ctccacctg caacagtcg cacctgctg gttcagtgt 
      tagtgttgt tttgtgcga aaatgggtc gtggaggtg 
      ttggttcaa caaggtccg aatttttgg ccgaagatt 
      cggttgatg atatcagat atcacagaa atgggggat 
      gttttgtga ccactaaaa gggccatag ctttcgttc 
      tggtagctg ttgtgtcct tgcttctgg ttgtgatct 
      tgatgtact actatggac ctagtcgga agttggaag 
      cgttgcaag acgacatga aagatatcg aggaactga 
      tcaacaaga caataaatg aggagcctg aagaaaata 
      tccgactac caacaaacc taaaaccac ttcattatc 
      ggcttagaa tttttccga ttctggacg aattttcgc 
      ctgataatt tcacttact cgggagaag tgaaaataa 
      tcattcggt gtttaacaa aaaccaaca aaatagttc 
      taaatttgg aagatttgg aagttagtg aacacaacg 
      tgactgtat cgacactta aaaccacaa ttttacgtt 
      acgaaagtt tggataaag agagtgata aagaccagc 
      ctgaaatgt accagaaaa attcgactc ataacagtt 
      cggtaataa ttgtggaaa gtgagaata attcaacgg 
      atgaaattc tgattggag ccgaagcta agtcgctca 
      tgatccagg aagtttata aagaagaga attataagt 
      tgtacatca ctatgaaaa atttgctag aagctgggc 
      ataattata cgattaata tcaaatttt ccgggaata 
      ttactaata atttggcgg ggttttatc ggacgagtt 
      ataaggatt tgtcagggc agagaaaat ggttggcaa 
      cgacttatt ttcaaccaa tttttgcaa gaagagtgt 
      ttccttgct ttgatgaac caaatttta aatcgtcct 
      ttgagatca gtattgcaa ggaggacta atatgacag 
      tgaggtcga atatgccac tcagggaaa ccgagccca 
      tcgctgaaa aaccgggat gggtttggg accactttg 
      aaaagtccc tcccaatgc ccacctact tggtctcat 
      tcacagttt gcgacttcc acaatttac acttaaatt 
      catccgaga ccgggcctg ttataaatt tgtgggccc 
      ctcagagcg acttaccca aagccaaat acgcgcttg 
      aagcagctc agagcattt taatcttct tggaagact 
      atctaggca ttaaatatc cactcccaa aaattgacc 
      ttctggcag ttccaaatt tcgcccgcg gttcaatgg 
      gaagttggg gcattttat cgtttcaaa aatcctcaa 
      tcctccttg aggaacatt cgagaaatt gggagttga 
      aacagcaca tcttcattg ctttggccc atgaattgg 
      cccaccagt ggtttggta atttggtca caatgaagt 
      ggtggaacg atttgtggt tgaacgaag gcattggga 
      gttttatgg ccgaagttg tgcaaacta gcttgaagc 
      ccaggtggc aaagcagca acgattttc ccgtgaggg 
      tgatgtaca aaagcttca gtttggaca gtttgaagt 
      caactcatc cgattcaga ccgatttca ccaaaacgg 
      tgcaaattg agcaaattt tcgatacga ttatacaaa 
      gcaaaggca cgagtttga tccggatgt tgaattata 
      cgttatcaa ggggggttt tccgacaag gactccaat 
      actatttga atagtttat cgtacaaat caaccgatc 
      aagactcgc tctggaaat cactaactg aaatcgcta 
      aaaattcgt cggttcttc cggacgatg tgacggtga 
      agagcttca tggagtcgt ggaccaaac aaaaaggat 
      atccttatg taaccgttg accggaatt atgacactg 
      gagaggctg ttattactc agcaaattt ttattcaag 
      ataatgtca acaataaca ctttgtggt ttattccgc 
      tgagcttta ccacaacgg atgatgtta ttcacaatg 
      cgtggttga aaaatgaaa gaaatacaa ctctgaaca 
      ttacgagtc cggggaata cttcttggg tgcttttta 
      acatcgatc aagcaggtt actttcgcg taaattacg 
      acattcaca attggaacc tcctcatcc gccaactgt 
      tcctcaacc cttcggaaa tccctgtgg caagtcgca 
      gccaactga tcgatgatg ctttcgaat tagcccaaa 
      tggagatcc tcaattaca gtattccac tgcgtctca 
      ccaaatacc tgacttgga aagaatcga attacattc 
      cctggacca cagcactca acagtttgg aggaaatcc 
      gattcatta tcaacaatt atgaataca ctggagctt 
      ttgaaacgt atatgacgc atctgatta gacacaaat 
      ttgattcgt tgggaacgc gtgaaaaac ccggggaac 
      cccaaaacg acaaaatcc tgaggtgga ggttgacga 
      aaatcgcct gcgaactga gatacgagc cgtgcattc 
      ggtgggcac agaaggagt tttacgcat ggatggaaa 
      atgcagatc ctgatgcga aaaatccga ttccgtttg 
      agtatcgct cgacggtgc aatgcattg gaattaaaa 
      cagggagtt tgaaggagt gggatttcc tatggaatc 
      gcactcttg agcctcaaa tatcgccag ctgatttga 
      aaactgcat atttgagtc tgggttgta cgcacgatc 
      cttggctca ttaacaggt acttggagt tgtctttaa 
      gtggcaata ttacgctgg aatacatcc cttatgtct 
      ggcagtcaa taaatcact ttgttggaa ttcggacag 
      gtttccatt ttttgaggt tgaactggg aacgcgttt 
      acagtactt atgaggatg ttgatttgg tgcttaatt 
      caatttgtc aagactttc tctcctatt tgtccacag 
      agattgatt tacaggatt tgactcaat tctataaaa 
      tacatcaga aggatttga aatcggtgg caccgctaa 
      tgcaagctg cagttgatt caatcaaaa ttcgaatca 
      gttggaggg aaaggcact tgaattcag tcgtcgcct 
      ggctgacca ataagtaac tgaatttat attcgtgtt 
      tttgtaata tattgttga ttaatttaa taaagtagt 
      ggtaagaaa