Status
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Gene information
General information
TC001356 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 15016911..15018683
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001356
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00243

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    Fertility
    egg chamber shape rounded - Number of animals showing the phenotype: 3
    egg chamber size decreased - Number of animals showing the phenotype: 3

    vitellogenic egg chamber late degenerated - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     larva segment number decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    larva appendages sometimes number decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    larva segment shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    larva cuticle dorsal not closed - Penetrance (percentage of animals/eggs on slide): more than 80%

    larva appendages mostly size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    antenna potentially transformed - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen bristle ectopically increased - Penetrance (percentage of animals/eggs on slide): more than 80%
    Comment: ventral ectopic stripes in AP direction.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 10

    wings blistered - Number of animals showing the phenotype: 10

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001356 RA
      MKEKSNPAV NDSNSNLTE DSGDEADET SSVGSTSTT 
SRSDTPTNR VRKGRRGKK KFNPLKVPK PIYKFTCNI 
KEDHGQPLF GTQFNHHLK EGQPLIFAA VGSNRVTIY 
ECPEGNGIK LLQCFADPD LDENYYTCA WTYDEETGK 
PLLAVAGSR GIVRILNPA SMTCMRHYI GHGHAINEL 
KFHPRDPNL LLSVSKDHA LRLWNIKTD VCIAIFGGV 
EGHRDEVLS ADFDLLGNR IMSCGMDHS LKLWKLNKE 
SMREAIKQS YQWNTTKST RPFNSLKEH FPDFSTRDI 
HRNYVDCVR WFGDFVLSK SCENCIVCW KPGRLEDDN 
LRKGETTST VIHRFEYKE CEIWFVRFA MDFWQKILA 
LGNQTGKIF VWDLDVDDP AQAKCYTLQ HPRCATAIR 
QTSLSRDAN ILLCVCDDG TIWRWDKVA
    • >TC001356 RB
      MASSPSRCE EKSNPAVND SNSNLTEDS GDEADETSS 
VGSTSTTSR SDTPTNRVR KGRRGKKKF NPLKVPKPI 
YKFTCNIKE DHGQPLFGT QFNHHLKEG QPLIFAAVG 
SNRVTIYEC PEGNGIKLL QCFADPDLD ENYYTCAWT 
YDEETGKPL LAVAGSRGI VRILNPASM TCMRHYIGH 
GHAINELKF HPRDPNLLL SVSKDHALR LWNIKTDVC 
IAIFGGVEG HRDEVLSAD FDLLGNRIM SCGMDHSLK 
LWKLNKESM REAIKQSYQ WNTTKSTRP FNSLKEHFP 
DFSTRDIHR NYVDCVRWF GDFVLSKSC ENCIVCWKP 
GRLEDDNLR KGETTSTVI HRFEYKECE IWFVRFAMD 
FWQKILALG NQTGKIFVW DLDVDDPAQ AKCYTLQHP 
RCATAIRQT SLSRDANIL LCVCDDGTI WRWDKVA
    CDS sequence
    • >TC001356 RA
      atgaaggaa aagtcgaat ccggcggtc aatgactcc 
aactcgaac ctaacggaa gactctggc gatgaggcg 
gatgagacc tcaagtgtt gggagcact tcgacgacg 
tccaggagc gacaccccc acaaacagg gtgcggaaa 
ggacggcgg ggtaagaaa aaattcaac ccgcttaaa 
gtccccaaa ccgatttac aagtttacg tgtaatatt 
aaggaggat catggacag ccacttttt ggcacccag 
ttcaaccac catttgaag gagggccaa ccgctgatt 
tttgcagcc gttgggagt aatagggtt acgatttat 
gagtgtcct gaggggaat gggatcaag cttttgcag 
tgttttgct gatcctgat ttagatgag aattattac 
acttgtgcc tggacttat gacgaagag acggggaag 
ccccttttg gcggttgct ggttcaaga ggcatagtt 
aggattttg aatccggcg tctatgacc tgcatgagg 
cattatata ggccatggg catgcgatt aacgagttg 
aagtttcat ccaagagac cccaatttg ttgctgtct 
gtgtccaaa gaccacgct ttgaggttg tggaatatt 
aagactgat gtctgtatt gccattttt gggggtgtt 
gaggggcac agagatgag gttttgagt gctgatttt 
gacttgttg ggcaacaga atcatgtct tgtggcatg 
gaccactcg ttaaaatta tggaaatta aacaaggag 
agtatgagg gaggctatt aaacagtcg taccaatgg 
aacacgact aagagcaca agaccgttc aattcgtta 
aaggagcat ttccctgac ttttccact agagacatt 
cacagaaat tacgtagat tgcgtgcgt tggtttggt 
gattttgtt ttgagtaaa tcgtgcgaa aactgcatc 
gtgtgttgg aagccagga cgtctggaa gatgataat 
ttgagaaag ggtgaaacc acgtccaca gtcattcac 
agatttgag tacaaagaa tgcgaaatt tggtttgtg 
agatttgct atggatttt tggcaaaaa attttggct 
ttgggtaat cagactggc aaaattttt gtgtgggat 
ttagacgtt gatgatcca gctcaagcc aaatgttac 
actctccag caccctaga tgcgctaca gcaatccga 
caaactagt ctcagcaga gacgccaat attttatta 
tgtgtgtgt gatgatggg acaatttgg aggtgggat 
aaagtagcg taa
    • >TC001356 RB
      atggcgtcc tcccccagc cgttgtgaa gaaaagtcg 
aatccggcg gtcaatgac tccaactcg aacctaacg 
gaagactct ggcgatgag gcggatgag acctcaagt 
gttgggagc acttcgacg acgtccagg agcgacacc 
cccacaaac agggtgcgg aaaggacgg cggggtaag 
aaaaaattc aacccgctt aaagtcccc aaaccgatt 
tacaagttt acgtgtaat attaaggag gatcatgga 
cagccactt tttggcacc cagttcaac caccatttg 
aaggagggc caaccgctg atttttgca gccgttggg 
agtaatagg gttacgatt tatgagtgt cctgagggg 
aatgggatc aagcttttg cagtgtttt gctgatcct 
gatttagat gagaattat tacacttgt gcctggact 
tatgacgaa gagacgggg aagcccctt ttggcggtt 
gctggttca agaggcata gttaggatt ttgaatccg 
gcgtctatg acctgcatg aggcattat ataggccat 
gggcatgcg attaacgag ttgaagttt catccaaga 
gaccccaat ttgttgctg tctgtgtcc aaagaccac 
gctttgagg ttgtggaat attaagact gatgtctgt 
attgccatt tttgggggt gttgagggg cacagagat 
gaggttttg agtgctgat tttgacttg ttgggcaac 
agaatcatg tcttgtggc atggaccac tcgttaaaa 
ttatggaaa ttaaacaag gagagtatg agggaggct 
attaaacag tcgtaccaa tggaacacg actaagagc 
acaagaccg ttcaattcg ttaaaggag catttccct 
gacttttcc actagagac attcacaga aattacgta 
gattgcgtg cgttggttt ggtgatttt gttttgagt 
aaatcgtgc gaaaactgc atcgtgtgt tggaagcca 
ggacgtctg gaagatgat aatttgaga aagggtgaa 
accacgtcc acagtcatt cacagattt gagtacaaa 
gaatgcgaa atttggttt gtgagattt gctatggat 
ttttggcaa aaaattttg gctttgggt aatcagact 
ggcaaaatt tttgtgtgg gatttagac gttgatgat 
ccagctcaa gccaaatgt tacactctc cagcaccct 
agatgcgct acagcaatc cgacaaact agtctcagc 
agagacgcc aatatttta ttatgtgtg tgtgatgat 
gggacaatt tggaggtgg gataaagta gcgtaa
    mRNA sequence
    • >TC001356 RA
      ccgttgtga agtaaggct ttaggcttt attttcaat 
tttaattta catttttat catgaagga aaagtcgaa 
tccggcggt caatgactc caactcgaa cctaacgga 
agactctgg cgatgaggc ggatgagac ctcaagtgt 
tgggagcac ttcgacgac gtccaggag cgacacccc 
cacaaacag ggtgcggaa aggacggcg gggtaagaa 
aaaattcaa cccgcttaa agtccccaa accgattta 
caagtttac gtgtaatat taaggagga tcatggaca 
gccactttt tggcaccca gttcaacca ccatttgaa 
ggagggcca accgctgat ttttgcagc cgttgggag 
taatagggt tacgattta tgagtgtcc tgaggggaa 
tgggatcaa gcttttgca gtgttttgc tgatcctga 
tttagatga gaattatta cacttgtgc ctggactta 
tgacgaaga gacggggaa gcccctttt ggcggttgc 
tggttcaag aggcatagt taggatttt gaatccggc 
gtctatgac ctgcatgag gcattatat aggccatgg 
gcatgcgat taacgagtt gaagtttca tccaagaga 
ccccaattt gttgctgtc tgtgtccaa agaccacgc 
tttgaggtt gtggaatat taagactga tgtctgtat 
tgccatttt tgggggtgt tgaggggca cagagatga 
ggttttgag tgctgattt tgacttgtt gggcaacag 
aatcatgtc ttgtggcat ggaccactc gttaaaatt 
atggaaatt aaacaagga gagtatgag ggaggctat 
taaacagtc gtaccaatg gaacacgac taagagcac 
aagaccgtt caattcgtt aaaggagca tttccctga 
cttttccac tagagacat tcacagaaa ttacgtaga 
ttgcgtgcg ttggtttgg tgattttgt tttgagtaa 
atcgtgcga aaactgcat cgtgtgttg gaagccagg 
acgtctgga agatgataa tttgagaaa gggtgaaac 
cacgtccac agtcattca cagatttga gtacaaaga 
atgcgaaat ttggtttgt gagatttgc tatggattt 
ttggcaaaa aattttggc tttgggtaa tcagactgg 
caaaatttt tgtgtggga tttagacgt tgatgatcc 
agctcaagc caaatgtta cactctcca gcaccctag 
atgcgctac agcaatccg acaaactag tctcagcag 
agacgccaa tattttatt atgtgtgtg tgatgatgg 
gacaatttg gaggtggga taaagtagc gtaactact 
tccggcttt gtatttttt taagataaa ataaactat 
ataaattga a
    • >TC001356 RB
      tcggtgttg cacactttg atggcgcgc tttcaacaa 
tggaaacaa tggcacaga ttagagaca atggcgtcc 
tcccccagc cgttgtgaa gaaaagtcg aatccggcg 
gtcaatgac tccaactcg aacctaacg gaagactct 
ggcgatgag gcggatgag acctcaagt gttgggagc 
acttcgacg acgtccagg agcgacacc cccacaaac 
agggtgcgg aaaggacgg cggggtaag aaaaaattc 
aacccgctt aaagtcccc aaaccgatt tacaagttt 
acgtgtaat attaaggag gatcatgga cagccactt 
tttggcacc cagttcaac caccatttg aaggagggc 
caaccgctg atttttgca gccgttggg agtaatagg 
gttacgatt tatgagtgt cctgagggg aatgggatc 
aagcttttg cagtgtttt gctgatcct gatttagat 
gagaattat tacacttgt gcctggact tatgacgaa 
gagacgggg aagcccctt ttggcggtt gctggttca 
agaggcata gttaggatt ttgaatccg gcgtctatg 
acctgcatg aggcattat ataggccat gggcatgcg 
attaacgag ttgaagttt catccaaga gaccccaat 
ttgttgctg tctgtgtcc aaagaccac gctttgagg 
ttgtggaat attaagact gatgtctgt attgccatt 
tttgggggt gttgagggg cacagagat gaggttttg 
agtgctgat tttgacttg ttgggcaac agaatcatg 
tcttgtggc atggaccac tcgttaaaa ttatggaaa 
ttaaacaag gagagtatg agggaggct attaaacag 
tcgtaccaa tggaacacg actaagagc acaagaccg 
ttcaattcg ttaaaggag catttccct gacttttcc 
actagagac attcacaga aattacgta gattgcgtg 
cgttggttt ggtgatttt gttttgagt aaatcgtgc 
gaaaactgc atcgtgtgt tggaagcca ggacgtctg 
gaagatgat aatttgaga aagggtgaa accacgtcc 
acagtcatt cacagattt gagtacaaa gaatgcgaa 
atttggttt gtgagattt gctatggat ttttggcaa 
aaaattttg gctttgggt aatcagact ggcaaaatt 
tttgtgtgg gatttagac gttgatgat ccagctcaa 
gccaaatgt tacactctc cagcaccct agatgcgct 
acagcaatc cgacaaact agtctcagc agagacgcc 
aatatttta ttatgtgtg tgtgatgat gggacaatt 
tggaggtgg gataaagta gcgtaacta cttccggct 
ttgtatttt tttaagata aaataaact atataaatt 
gaa

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY