Status
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Gene information
General information
TC001356 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 15016911..15018683
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001356
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00243

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Fertility
    egg chamber shape rounded - Number of animals showing the phenotype: 3
    egg chamber size decreased - Number of animals showing the phenotype: 3

    vitellogenic egg chamber late degenerated - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva segment number decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    larva appendages sometimes number decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    larva segment shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    larva cuticle dorsal not closed - Penetrance (percentage of animals/eggs on slide): more than 80%

    larva appendages mostly size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    antenna potentially transformed - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen bristle ectopically increased - Penetrance (percentage of animals/eggs on slide): more than 80%
    Comment: ventral ectopic stripes in AP direction.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection



    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 10

    wings blistered - Number of animals showing the phenotype: 10

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001356 RA
      MKEKSNPAV NDSNSNLTE DSGDEADET SSVGSTSTT 
      SRSDTPTNR VRKGRRGKK KFNPLKVPK PIYKFTCNI 
      KEDHGQPLF GTQFNHHLK EGQPLIFAA VGSNRVTIY 
      ECPEGNGIK LLQCFADPD LDENYYTCA WTYDEETGK 
      PLLAVAGSR GIVRILNPA SMTCMRHYI GHGHAINEL 
      KFHPRDPNL LLSVSKDHA LRLWNIKTD VCIAIFGGV 
      EGHRDEVLS ADFDLLGNR IMSCGMDHS LKLWKLNKE 
      SMREAIKQS YQWNTTKST RPFNSLKEH FPDFSTRDI 
      HRNYVDCVR WFGDFVLSK SCENCIVCW KPGRLEDDN 
      LRKGETTST VIHRFEYKE CEIWFVRFA MDFWQKILA 
      LGNQTGKIF VWDLDVDDP AQAKCYTLQ HPRCATAIR 
      QTSLSRDAN ILLCVCDDG TIWRWDKVA
    • >TC001356 RB
      MASSPSRCE EKSNPAVND SNSNLTEDS GDEADETSS 
      VGSTSTTSR SDTPTNRVR KGRRGKKKF NPLKVPKPI 
      YKFTCNIKE DHGQPLFGT QFNHHLKEG QPLIFAAVG 
      SNRVTIYEC PEGNGIKLL QCFADPDLD ENYYTCAWT 
      YDEETGKPL LAVAGSRGI VRILNPASM TCMRHYIGH 
      GHAINELKF HPRDPNLLL SVSKDHALR LWNIKTDVC 
      IAIFGGVEG HRDEVLSAD FDLLGNRIM SCGMDHSLK 
      LWKLNKESM REAIKQSYQ WNTTKSTRP FNSLKEHFP 
      DFSTRDIHR NYVDCVRWF GDFVLSKSC ENCIVCWKP 
      GRLEDDNLR KGETTSTVI HRFEYKECE IWFVRFAMD 
      FWQKILALG NQTGKIFVW DLDVDDPAQ AKCYTLQHP 
      RCATAIRQT SLSRDANIL LCVCDDGTI WRWDKVA
    CDS sequence
    • >TC001356 RA
      atgaaggaa aagtcgaat ccggcggtc aatgactcc 
      aactcgaac ctaacggaa gactctggc gatgaggcg 
      gatgagacc tcaagtgtt gggagcact tcgacgacg 
      tccaggagc gacaccccc acaaacagg gtgcggaaa 
      ggacggcgg ggtaagaaa aaattcaac ccgcttaaa 
      gtccccaaa ccgatttac aagtttacg tgtaatatt 
      aaggaggat catggacag ccacttttt ggcacccag 
      ttcaaccac catttgaag gagggccaa ccgctgatt 
      tttgcagcc gttgggagt aatagggtt acgatttat 
      gagtgtcct gaggggaat gggatcaag cttttgcag 
      tgttttgct gatcctgat ttagatgag aattattac 
      acttgtgcc tggacttat gacgaagag acggggaag 
      ccccttttg gcggttgct ggttcaaga ggcatagtt 
      aggattttg aatccggcg tctatgacc tgcatgagg 
      cattatata ggccatggg catgcgatt aacgagttg 
      aagtttcat ccaagagac cccaatttg ttgctgtct 
      gtgtccaaa gaccacgct ttgaggttg tggaatatt 
      aagactgat gtctgtatt gccattttt gggggtgtt 
      gaggggcac agagatgag gttttgagt gctgatttt 
      gacttgttg ggcaacaga atcatgtct tgtggcatg 
      gaccactcg ttaaaatta tggaaatta aacaaggag 
      agtatgagg gaggctatt aaacagtcg taccaatgg 
      aacacgact aagagcaca agaccgttc aattcgtta 
      aaggagcat ttccctgac ttttccact agagacatt 
      cacagaaat tacgtagat tgcgtgcgt tggtttggt 
      gattttgtt ttgagtaaa tcgtgcgaa aactgcatc 
      gtgtgttgg aagccagga cgtctggaa gatgataat 
      ttgagaaag ggtgaaacc acgtccaca gtcattcac 
      agatttgag tacaaagaa tgcgaaatt tggtttgtg 
      agatttgct atggatttt tggcaaaaa attttggct 
      ttgggtaat cagactggc aaaattttt gtgtgggat 
      ttagacgtt gatgatcca gctcaagcc aaatgttac 
      actctccag caccctaga tgcgctaca gcaatccga 
      caaactagt ctcagcaga gacgccaat attttatta 
      tgtgtgtgt gatgatggg acaatttgg aggtgggat 
      aaagtagcg taa
    • >TC001356 RB
      atggcgtcc tcccccagc cgttgtgaa gaaaagtcg 
      aatccggcg gtcaatgac tccaactcg aacctaacg 
      gaagactct ggcgatgag gcggatgag acctcaagt 
      gttgggagc acttcgacg acgtccagg agcgacacc 
      cccacaaac agggtgcgg aaaggacgg cggggtaag 
      aaaaaattc aacccgctt aaagtcccc aaaccgatt 
      tacaagttt acgtgtaat attaaggag gatcatgga 
      cagccactt tttggcacc cagttcaac caccatttg 
      aaggagggc caaccgctg atttttgca gccgttggg 
      agtaatagg gttacgatt tatgagtgt cctgagggg 
      aatgggatc aagcttttg cagtgtttt gctgatcct 
      gatttagat gagaattat tacacttgt gcctggact 
      tatgacgaa gagacgggg aagcccctt ttggcggtt 
      gctggttca agaggcata gttaggatt ttgaatccg 
      gcgtctatg acctgcatg aggcattat ataggccat 
      gggcatgcg attaacgag ttgaagttt catccaaga 
      gaccccaat ttgttgctg tctgtgtcc aaagaccac 
      gctttgagg ttgtggaat attaagact gatgtctgt 
      attgccatt tttgggggt gttgagggg cacagagat 
      gaggttttg agtgctgat tttgacttg ttgggcaac 
      agaatcatg tcttgtggc atggaccac tcgttaaaa 
      ttatggaaa ttaaacaag gagagtatg agggaggct 
      attaaacag tcgtaccaa tggaacacg actaagagc 
      acaagaccg ttcaattcg ttaaaggag catttccct 
      gacttttcc actagagac attcacaga aattacgta 
      gattgcgtg cgttggttt ggtgatttt gttttgagt 
      aaatcgtgc gaaaactgc atcgtgtgt tggaagcca 
      ggacgtctg gaagatgat aatttgaga aagggtgaa 
      accacgtcc acagtcatt cacagattt gagtacaaa 
      gaatgcgaa atttggttt gtgagattt gctatggat 
      ttttggcaa aaaattttg gctttgggt aatcagact 
      ggcaaaatt tttgtgtgg gatttagac gttgatgat 
      ccagctcaa gccaaatgt tacactctc cagcaccct 
      agatgcgct acagcaatc cgacaaact agtctcagc 
      agagacgcc aatatttta ttatgtgtg tgtgatgat 
      gggacaatt tggaggtgg gataaagta gcgtaa
    mRNA sequence
    • >TC001356 RA
      ccgttgtga agtaaggct ttaggcttt attttcaat 
      tttaattta catttttat catgaagga aaagtcgaa 
      tccggcggt caatgactc caactcgaa cctaacgga 
      agactctgg cgatgaggc ggatgagac ctcaagtgt 
      tgggagcac ttcgacgac gtccaggag cgacacccc 
      cacaaacag ggtgcggaa aggacggcg gggtaagaa 
      aaaattcaa cccgcttaa agtccccaa accgattta 
      caagtttac gtgtaatat taaggagga tcatggaca 
      gccactttt tggcaccca gttcaacca ccatttgaa 
      ggagggcca accgctgat ttttgcagc cgttgggag 
      taatagggt tacgattta tgagtgtcc tgaggggaa 
      tgggatcaa gcttttgca gtgttttgc tgatcctga 
      tttagatga gaattatta cacttgtgc ctggactta 
      tgacgaaga gacggggaa gcccctttt ggcggttgc 
      tggttcaag aggcatagt taggatttt gaatccggc 
      gtctatgac ctgcatgag gcattatat aggccatgg 
      gcatgcgat taacgagtt gaagtttca tccaagaga 
      ccccaattt gttgctgtc tgtgtccaa agaccacgc 
      tttgaggtt gtggaatat taagactga tgtctgtat 
      tgccatttt tgggggtgt tgaggggca cagagatga 
      ggttttgag tgctgattt tgacttgtt gggcaacag 
      aatcatgtc ttgtggcat ggaccactc gttaaaatt 
      atggaaatt aaacaagga gagtatgag ggaggctat 
      taaacagtc gtaccaatg gaacacgac taagagcac 
      aagaccgtt caattcgtt aaaggagca tttccctga 
      cttttccac tagagacat tcacagaaa ttacgtaga 
      ttgcgtgcg ttggtttgg tgattttgt tttgagtaa 
      atcgtgcga aaactgcat cgtgtgttg gaagccagg 
      acgtctgga agatgataa tttgagaaa gggtgaaac 
      cacgtccac agtcattca cagatttga gtacaaaga 
      atgcgaaat ttggtttgt gagatttgc tatggattt 
      ttggcaaaa aattttggc tttgggtaa tcagactgg 
      caaaatttt tgtgtggga tttagacgt tgatgatcc 
      agctcaagc caaatgtta cactctcca gcaccctag 
      atgcgctac agcaatccg acaaactag tctcagcag 
      agacgccaa tattttatt atgtgtgtg tgatgatgg 
      gacaatttg gaggtggga taaagtagc gtaactact 
      tccggcttt gtatttttt taagataaa ataaactat 
      ataaattga a
    • >TC001356 RB
      tcggtgttg cacactttg atggcgcgc tttcaacaa 
      tggaaacaa tggcacaga ttagagaca atggcgtcc 
      tcccccagc cgttgtgaa gaaaagtcg aatccggcg 
      gtcaatgac tccaactcg aacctaacg gaagactct 
      ggcgatgag gcggatgag acctcaagt gttgggagc 
      acttcgacg acgtccagg agcgacacc cccacaaac 
      agggtgcgg aaaggacgg cggggtaag aaaaaattc 
      aacccgctt aaagtcccc aaaccgatt tacaagttt 
      acgtgtaat attaaggag gatcatgga cagccactt 
      tttggcacc cagttcaac caccatttg aaggagggc 
      caaccgctg atttttgca gccgttggg agtaatagg 
      gttacgatt tatgagtgt cctgagggg aatgggatc 
      aagcttttg cagtgtttt gctgatcct gatttagat 
      gagaattat tacacttgt gcctggact tatgacgaa 
      gagacgggg aagcccctt ttggcggtt gctggttca 
      agaggcata gttaggatt ttgaatccg gcgtctatg 
      acctgcatg aggcattat ataggccat gggcatgcg 
      attaacgag ttgaagttt catccaaga gaccccaat 
      ttgttgctg tctgtgtcc aaagaccac gctttgagg 
      ttgtggaat attaagact gatgtctgt attgccatt 
      tttgggggt gttgagggg cacagagat gaggttttg 
      agtgctgat tttgacttg ttgggcaac agaatcatg 
      tcttgtggc atggaccac tcgttaaaa ttatggaaa 
      ttaaacaag gagagtatg agggaggct attaaacag 
      tcgtaccaa tggaacacg actaagagc acaagaccg 
      ttcaattcg ttaaaggag catttccct gacttttcc 
      actagagac attcacaga aattacgta gattgcgtg 
      cgttggttt ggtgatttt gttttgagt aaatcgtgc 
      gaaaactgc atcgtgtgt tggaagcca ggacgtctg 
      gaagatgat aatttgaga aagggtgaa accacgtcc 
      acagtcatt cacagattt gagtacaaa gaatgcgaa 
      atttggttt gtgagattt gctatggat ttttggcaa 
      aaaattttg gctttgggt aatcagact ggcaaaatt 
      tttgtgtgg gatttagac gttgatgat ccagctcaa 
      gccaaatgt tacactctc cagcaccct agatgcgct 
      acagcaatc cgacaaact agtctcagc agagacgcc 
      aatatttta ttatgtgtg tgtgatgat gggacaatt 
      tggaggtgg gataaagta gcgtaacta cttccggct 
      ttgtatttt tttaagata aaataaact atataaatt 
      gaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY