General information | |||
TC001356 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 15016911..15018683 | |
Sequence information | |||
Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC001356 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00243 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
egg chamber size decreased - Number of animals showing the phenotype: 3
vitellogenic egg chamber late degenerated - Number of animals showing the phenotype: 3
larva segment number decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
larva appendages sometimes number decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
larva segment shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
larva cuticle dorsal not closed - Penetrance (percentage of animals/eggs on slide): more than 80%
larva appendages mostly size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
antenna potentially transformed - Penetrance (percentage of animals/eggs on slide): more than 80%
abdomen bristle ectopically increased - Penetrance (percentage of animals/eggs on slide): more than 80%
Comment: ventral ectopic stripes in AP direction. |
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC001356 RA
MKEKSNPAV NDSNSNLTE DSGDEADET SSVGSTSTT SRSDTPTNR VRKGRRGKK KFNPLKVPK PIYKFTCNI KEDHGQPLF GTQFNHHLK EGQPLIFAA VGSNRVTIY ECPEGNGIK LLQCFADPD LDENYYTCA WTYDEETGK PLLAVAGSR GIVRILNPA SMTCMRHYI GHGHAINEL KFHPRDPNL LLSVSKDHA LRLWNIKTD VCIAIFGGV EGHRDEVLS ADFDLLGNR IMSCGMDHS LKLWKLNKE SMREAIKQS YQWNTTKST RPFNSLKEH FPDFSTRDI HRNYVDCVR WFGDFVLSK SCENCIVCW KPGRLEDDN LRKGETTST VIHRFEYKE CEIWFVRFA MDFWQKILA LGNQTGKIF VWDLDVDDP AQAKCYTLQ HPRCATAIR QTSLSRDAN ILLCVCDDG TIWRWDKVA
- >TC001356 RB
MASSPSRCE EKSNPAVND SNSNLTEDS GDEADETSS VGSTSTTSR SDTPTNRVR KGRRGKKKF NPLKVPKPI YKFTCNIKE DHGQPLFGT QFNHHLKEG QPLIFAAVG SNRVTIYEC PEGNGIKLL QCFADPDLD ENYYTCAWT YDEETGKPL LAVAGSRGI VRILNPASM TCMRHYIGH GHAINELKF HPRDPNLLL SVSKDHALR LWNIKTDVC IAIFGGVEG HRDEVLSAD FDLLGNRIM SCGMDHSLK LWKLNKESM REAIKQSYQ WNTTKSTRP FNSLKEHFP DFSTRDIHR NYVDCVRWF GDFVLSKSC ENCIVCWKP GRLEDDNLR KGETTSTVI HRFEYKECE IWFVRFAMD FWQKILALG NQTGKIFVW DLDVDDPAQ AKCYTLQHP RCATAIRQT SLSRDANIL LCVCDDGTI WRWDKVA
- >TC001356 RA
atgaaggaa aagtcgaat ccggcggtc aatgactcc aactcgaac ctaacggaa gactctggc gatgaggcg gatgagacc tcaagtgtt gggagcact tcgacgacg tccaggagc gacaccccc acaaacagg gtgcggaaa ggacggcgg ggtaagaaa aaattcaac ccgcttaaa gtccccaaa ccgatttac aagtttacg tgtaatatt aaggaggat catggacag ccacttttt ggcacccag ttcaaccac catttgaag gagggccaa ccgctgatt tttgcagcc gttgggagt aatagggtt acgatttat gagtgtcct gaggggaat gggatcaag cttttgcag tgttttgct gatcctgat ttagatgag aattattac acttgtgcc tggacttat gacgaagag acggggaag ccccttttg gcggttgct ggttcaaga ggcatagtt aggattttg aatccggcg tctatgacc tgcatgagg cattatata ggccatggg catgcgatt aacgagttg aagtttcat ccaagagac cccaatttg ttgctgtct gtgtccaaa gaccacgct ttgaggttg tggaatatt aagactgat gtctgtatt gccattttt gggggtgtt gaggggcac agagatgag gttttgagt gctgatttt gacttgttg ggcaacaga atcatgtct tgtggcatg gaccactcg ttaaaatta tggaaatta aacaaggag agtatgagg gaggctatt aaacagtcg taccaatgg aacacgact aagagcaca agaccgttc aattcgtta aaggagcat ttccctgac ttttccact agagacatt cacagaaat tacgtagat tgcgtgcgt tggtttggt gattttgtt ttgagtaaa tcgtgcgaa aactgcatc gtgtgttgg aagccagga cgtctggaa gatgataat ttgagaaag ggtgaaacc acgtccaca gtcattcac agatttgag tacaaagaa tgcgaaatt tggtttgtg agatttgct atggatttt tggcaaaaa attttggct ttgggtaat cagactggc aaaattttt gtgtgggat ttagacgtt gatgatcca gctcaagcc aaatgttac actctccag caccctaga tgcgctaca gcaatccga caaactagt ctcagcaga gacgccaat attttatta tgtgtgtgt gatgatggg acaatttgg aggtgggat aaagtagcg taa
- >TC001356 RB
atggcgtcc tcccccagc cgttgtgaa gaaaagtcg aatccggcg gtcaatgac tccaactcg aacctaacg gaagactct ggcgatgag gcggatgag acctcaagt gttgggagc acttcgacg acgtccagg agcgacacc cccacaaac agggtgcgg aaaggacgg cggggtaag aaaaaattc aacccgctt aaagtcccc aaaccgatt tacaagttt acgtgtaat attaaggag gatcatgga cagccactt tttggcacc cagttcaac caccatttg aaggagggc caaccgctg atttttgca gccgttggg agtaatagg gttacgatt tatgagtgt cctgagggg aatgggatc aagcttttg cagtgtttt gctgatcct gatttagat gagaattat tacacttgt gcctggact tatgacgaa gagacgggg aagcccctt ttggcggtt gctggttca agaggcata gttaggatt ttgaatccg gcgtctatg acctgcatg aggcattat ataggccat gggcatgcg attaacgag ttgaagttt catccaaga gaccccaat ttgttgctg tctgtgtcc aaagaccac gctttgagg ttgtggaat attaagact gatgtctgt attgccatt tttgggggt gttgagggg cacagagat gaggttttg agtgctgat tttgacttg ttgggcaac agaatcatg tcttgtggc atggaccac tcgttaaaa ttatggaaa ttaaacaag gagagtatg agggaggct attaaacag tcgtaccaa tggaacacg actaagagc acaagaccg ttcaattcg ttaaaggag catttccct gacttttcc actagagac attcacaga aattacgta gattgcgtg cgttggttt ggtgatttt gttttgagt aaatcgtgc gaaaactgc atcgtgtgt tggaagcca ggacgtctg gaagatgat aatttgaga aagggtgaa accacgtcc acagtcatt cacagattt gagtacaaa gaatgcgaa atttggttt gtgagattt gctatggat ttttggcaa aaaattttg gctttgggt aatcagact ggcaaaatt tttgtgtgg gatttagac gttgatgat ccagctcaa gccaaatgt tacactctc cagcaccct agatgcgct acagcaatc cgacaaact agtctcagc agagacgcc aatatttta ttatgtgtg tgtgatgat gggacaatt tggaggtgg gataaagta gcgtaa
- >TC001356 RA
ccgttgtga agtaaggct ttaggcttt attttcaat tttaattta catttttat catgaagga aaagtcgaa tccggcggt caatgactc caactcgaa cctaacgga agactctgg cgatgaggc ggatgagac ctcaagtgt tgggagcac ttcgacgac gtccaggag cgacacccc cacaaacag ggtgcggaa aggacggcg gggtaagaa aaaattcaa cccgcttaa agtccccaa accgattta caagtttac gtgtaatat taaggagga tcatggaca gccactttt tggcaccca gttcaacca ccatttgaa ggagggcca accgctgat ttttgcagc cgttgggag taatagggt tacgattta tgagtgtcc tgaggggaa tgggatcaa gcttttgca gtgttttgc tgatcctga tttagatga gaattatta cacttgtgc ctggactta tgacgaaga gacggggaa gcccctttt ggcggttgc tggttcaag aggcatagt taggatttt gaatccggc gtctatgac ctgcatgag gcattatat aggccatgg gcatgcgat taacgagtt gaagtttca tccaagaga ccccaattt gttgctgtc tgtgtccaa agaccacgc tttgaggtt gtggaatat taagactga tgtctgtat tgccatttt tgggggtgt tgaggggca cagagatga ggttttgag tgctgattt tgacttgtt gggcaacag aatcatgtc ttgtggcat ggaccactc gttaaaatt atggaaatt aaacaagga gagtatgag ggaggctat taaacagtc gtaccaatg gaacacgac taagagcac aagaccgtt caattcgtt aaaggagca tttccctga cttttccac tagagacat tcacagaaa ttacgtaga ttgcgtgcg ttggtttgg tgattttgt tttgagtaa atcgtgcga aaactgcat cgtgtgttg gaagccagg acgtctgga agatgataa tttgagaaa gggtgaaac cacgtccac agtcattca cagatttga gtacaaaga atgcgaaat ttggtttgt gagatttgc tatggattt ttggcaaaa aattttggc tttgggtaa tcagactgg caaaatttt tgtgtggga tttagacgt tgatgatcc agctcaagc caaatgtta cactctcca gcaccctag atgcgctac agcaatccg acaaactag tctcagcag agacgccaa tattttatt atgtgtgtg tgatgatgg gacaatttg gaggtggga taaagtagc gtaactact tccggcttt gtatttttt taagataaa ataaactat ataaattga a
- >TC001356 RB
tcggtgttg cacactttg atggcgcgc tttcaacaa tggaaacaa tggcacaga ttagagaca atggcgtcc tcccccagc cgttgtgaa gaaaagtcg aatccggcg gtcaatgac tccaactcg aacctaacg gaagactct ggcgatgag gcggatgag acctcaagt gttgggagc acttcgacg acgtccagg agcgacacc cccacaaac agggtgcgg aaaggacgg cggggtaag aaaaaattc aacccgctt aaagtcccc aaaccgatt tacaagttt acgtgtaat attaaggag gatcatgga cagccactt tttggcacc cagttcaac caccatttg aaggagggc caaccgctg atttttgca gccgttggg agtaatagg gttacgatt tatgagtgt cctgagggg aatgggatc aagcttttg cagtgtttt gctgatcct gatttagat gagaattat tacacttgt gcctggact tatgacgaa gagacgggg aagcccctt ttggcggtt gctggttca agaggcata gttaggatt ttgaatccg gcgtctatg acctgcatg aggcattat ataggccat gggcatgcg attaacgag ttgaagttt catccaaga gaccccaat ttgttgctg tctgtgtcc aaagaccac gctttgagg ttgtggaat attaagact gatgtctgt attgccatt tttgggggt gttgagggg cacagagat gaggttttg agtgctgat tttgacttg ttgggcaac agaatcatg tcttgtggc atggaccac tcgttaaaa ttatggaaa ttaaacaag gagagtatg agggaggct attaaacag tcgtaccaa tggaacacg actaagagc acaagaccg ttcaattcg ttaaaggag catttccct gacttttcc actagagac attcacaga aattacgta gattgcgtg cgttggttt ggtgatttt gttttgagt aaatcgtgc gaaaactgc atcgtgtgt tggaagcca ggacgtctg gaagatgat aatttgaga aagggtgaa accacgtcc acagtcatt cacagattt gagtacaaa gaatgcgaa atttggttt gtgagattt gctatggat ttttggcaa aaaattttg gctttgggt aatcagact ggcaaaatt tttgtgtgg gatttagac gttgatgat ccagctcaa gccaaatgt tacactctc cagcaccct agatgcgct acagcaatc cgacaaact agtctcagc agagacgcc aatatttta ttatgtgtg tgtgatgat gggacaatt tggaggtgg gataaagta gcgtaacta cttccggct ttgtatttt tttaagata aaataaact atataaatt gaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
