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Gene information
General information
TC000193 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14920027..14922042
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000193
    iBeetle screen iB_00242

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Adult morphology
    low validity due to technical reasons - should be repeated: ovary phenotype not scorable,
    animals lost.
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000193 RA
      MAELQITPK RTIGHNHPT FIIAEIGQN HQGDVKIAK 
      KLIHLAKES GADCVKFQK TCLTEKFNK SALDRPYRG 
      PHSWGATYG EHKQFLEFT KEQFRELQS FAAEIGILF 
      TASAMDSQS LRFLASLNV PFIKIGSGD SNNLLLIQE 
      AAAMNVPLV ISTGMQDLG GVRATYECV AQYHKKFAL 
      LHCVSAYPT PLQEINLNV IKLYETEFP DVVIGYSGH 
      ELGIEASVA AVAVGAKII ERHVTLDKT QKGSDHQCS 
      LEPQELKLL IEKIRELDI TLGKPVKAF QASERPCYA 
      KLGKSLVAA TTLRKGDIL QHENIKIKV AEPKGIDAS 
      LLKDVIGKH VKGVINEDE SILQENLE
    CDS sequence
    • >TC000193 RA
      atggccgaa ttacaaatc actcccaaa cgcacaatt 
      ggccataat caccctacc tttatcatc gccgaaatt 
      ggccaaaac caccaaggc gacgtaaag atcgccaaa 
      aaattaatt catttggcg aaggaaagt ggggccgat 
      tgtgttaaa ttccagaaa acctgtctc actgaaaaa 
      ttcaataaa tcggcccta gacaggcca taccgtggc 
      ccccattca tggggggcc acctacggc gaacacaaa 
      caatttctg gaatttaca aaagaacag tttcgcgaa 
      ttgcaaagt tttgccgcc gaaattggt attttattc 
      acagcgtca gcaatggat tcacaatct ttacgattt 
      cttgccagt ttgaacgtt ccgtttatt aagataggg 
      tccggtgat tctaataat ttgctactg attcaagaa 
      gccgcagca atgaatgtg cctttggtc atatcaaca 
      ggaatgcaa gacctgggg ggtgtgaga gccacttat 
      gagtgtgtc gctcagtat cataaaaaa tttgcgcta 
      cttcattgc gtttctgca tatcctaca cctctccag 
      gagataaat cttaacgtt attaaattg tacgagacg 
      gaatttcct gatgttgtg atcggttat tcgggacat 
      gagctgggg attgaggcc agtgtggct gctgtggca 
      gtgggggct aaaattatt gagcgtcat gtaacactt 
      gataaaacc cagaaaggg tcagaccat caatgttca 
      ttagaacca caggaactt aagctttta attgaaaaa 
      ataagagag ttggacatt actttaggt aagcctgta 
      aaggctttt caagcttcc gaacgtccg tgttatgcc 
      aaactcggc aaaagttta gttgctgcc acaactcta 
      cgaaagggc gatattttg caacatgag aatatcaaa 
      atcaaagtt gctgagccg aaaggaatc gacgcttct 
      ttgctcaaa gatgtgata gggaaacat gttaaagga 
      gttattaat gaggatgaa tcaatttta caagaaaat 
      ttggaataa
    mRNA sequence
    • >TC000193 RA
      ttaaaatgg ccgaattac aaatcactc ccaaacgca 
      caattggcc ataatcacc ctaccttta tcatcgccg 
      aaattggcc aaaaccacc aaggcgacg taaagatcg 
      ccaaaaaat taattcatt tggcgaagg aaagtgggg 
      ccgattgtg ttaaattcc agaaaacct gtctcactg 
      aaaaattca ataaatcgg ccctagaca ggccatacc 
      gtggccccc attcatggg gggccacct acggcgaac 
      acaaacaat ttctggaat ttacaaaag aacagtttc 
      gcgaattgc aaagttttg ccgccgaaa ttggtattt 
      tattcacag cgtcagcaa tggattcac aatctttac 
      gatttcttg ccagtttga acgttccgt ttattaaga 
      tagggtccg gtgattcta ataatttgc tactgattc 
      aagaagccg cagcaatga atgtgcctt tggtcatat 
      caacaggaa tgcaagacc tggggggtg tgagagcca 
      cttatgagt gtgtcgctc agtatcata aaaaatttg 
      cgctacttc attgcgttt ctgcatatc ctacacctc 
      tccaggaga taaatctta acgttatta aattgtacg 
      agacggaat ttcctgatg ttgtgatcg gttattcgg 
      gacatgagc tggggattg aggccagtg tggctgctg 
      tggcagtgg gggctaaaa ttattgagc gtcatgtaa 
      cacttgata aaacccaga aagggtcag accatcaat 
      gttcattag aaccacagg aacttaagc ttttaattg 
      aaaaaataa gagagttgg acattactt taggtaagc 
      ctgtaaagg cttttcaag cttccgaac gtccgtgtt 
      atgccaaac tcggcaaaa gtttagttg ctgccacaa 
      ctctacgaa agggcgata ttttgcaac atgagaata 
      tcaaaatca aagttgctg agccgaaag gaatcgacg 
      cttctttgc tcaaagatg tgataggga aacatgtta 
      aaggagtta ttaatgagg atgaatcaa ttttacaag 
      aaaatttgg aataattta ttgaataaa tgatttaca 
      aatcacttt tttcgttca tactgccag acatagacc 
      ggtgcttgg gtgcggtta cctctccgc acacgtttt 
      gggggcaaa acgttcttc ctattctcc acgtgtctt 
      tttatttca attttttgt tgaatgact gatagttcc 
      ttctccctg ttgattctc ttagctagt tcgttgtac 
      aaatgtttc tttttcttc gccgcttca gctaaatca 
      tcaaggttc acatcaggc aactctttt ttggacagt 
      tgctccaat ttcttcttc ttcaattcg tccttgtct 
      agattcttg gtgaaataa atatgttta ttcttaaca 
      ctatgatcc acacttgct aaatgcaac tgtgcttga 
      agcttctca attttcttc aattcttgt gtccgtttc 
      gtcgtgatg tacttcaaa tcttggctt cgcattagc 
      cttatttgt tcaggtgtg tcttcggct tcagtctct 
      ttttcaaag tgcatgcca ttttccgtt tttgagttg 
      atcatgtgg tggtaaaac tcatcaggg tttttgttg 
      agagctcgt ttacgcaaa agtttaagg gtttctttc 
      ttttcgttg aagtcttgg gctcgcttg acataatcc 
      ttctttttt tctaataga ccgaggtgc ttgcgttct 
      tctggctgg tgccgctct ttgtgactt ttctggtgg 
      gcctttgcg gcctttttc caaacagac attttcaaa 
      aaaatcaac acatgtgtc aatgatgag aaaccaacc 
      tcaaaattg ctattttga caactacta agtctgaca 
      atgttgcca aatgttcaa aaataatat agttcaggt 
      gtcata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY