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Gene information
General information
TC001328 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14546685..14548549
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001328
    iBeetle screen iB_00240

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Adult morphology
    low validity due to technical reasons - should be repeated: ovary phenotype not scorable,
    animals lost
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 75.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection



    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    delayed (still larva) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001328 RA
      MVIRNLHRL SRLFQPVSV VPKNAEVKR HDITSKSQR 
      LMLELGIIR QASPGCFHF LPLGVKSLE KLTRLVDEE 
      MKKIGGQKI IFPTLTNAK LWEKTGRLE TVSSELFQI 
      KDRHDQSYI LSPTHEEAA SNLLASLAP ISYKHLPLK 
      LYQITSKYR DEMKPRFGL MRGKQFLMK DMYSFDTDL 
      ETAKHTYDE ICKSYDRIF RRIGIDFVK VVGCSGHMG 
      GELSHEYHY KADVGEDKI LTCNKCNYS ANTEVSGEE 
      KCPNCGNTS VKIHLGIEV GHTFLLGEK YSKPLNATF 
      LNNHGKPDV LQMGSYGLG LSRILAAVI EVMSSEQEI 
      RWPQILSPY SVIILPPKS GSKEETHIK DSGLVDSLY 
      EKIETTCNL QDNVLIDDR TNFTIGRRF LEAKKNGYP 
      FIVTLGKKI VENPLLYEI TNLKTNETL FLSENDLMN 
      YLRSQLSSF SSDSDNK
    CDS sequence
    • >TC001328 RA
      atggttatt cggaacttg caccgactc tcccgcctt 
      ttccagccg gtttcggtg gtccccaaa aatgcggaa 
      gtcaagcgg cacgatatc acttcgaag agtcaacga 
      ttaatgcta gagcttggg attataaga caagcgagt 
      ccgggttgt ttccatttt ttgccatta ggggtcaag 
      tctttggag aaactaaca aggcttgtt gatgaggaa 
      atgaaaaaa attgggggg caaaaaatt atttttccc 
      acgttaact aacgcgaaa ctgtgggaa aaaacaggc 
      agattggag actgtttcc agtgagctt ttccaaatt 
      aaagaccgg catgaccag agttatatt ttaagccct 
      acacatgag gaggccgcg agtaattta cttgcgtcg 
      ttggcgccg atttcgtac aagcactta cctttaaaa 
      ctgtatcag attacaagt aaatatagg gacgagatg 
      aaacccaga tttgggtta atgagggga aagcagttt 
      cttatgaaa gacatgtat tcgtttgat accgacctc 
      gaaacggcc aaacacact tacgatgaa atttgtaaa 
      agttacgat cggattttt aggagaatt gggattgat 
      tttgtcaaa gtggtgggt tgttcggga catatgggg 
      ggcgaatta tcgcacgaa taccactac aaagcggac 
      gtaggcgaa gataaaatt ttaacgtgc aacaaatgc 
      aactattcg gcaaatacc gaagtttca ggggaggaa 
      aaatgcccc aattgtggg aacacttca gtcaaaatt 
      cacttaggg atagaagtt ggtcacact tttcttttg 
      ggcgaaaaa tactcaaaa ccgcttaat gccactttt 
      ttgaataat cacggcaaa ccggacgtg ttacaaatg 
      ggcagttat ggactgggg ttatcacgg attttagct 
      gccgtaatt gaagtcatg tcaagtgaa caagaaatc 
      aggtggccc cagatttta tcaccttac agcgtcatt 
      attttgcct ccgaaaagc ggaagtaag gaagaaaca 
      cacattaag gattcgggt ctagttgac tcactttac 
      gaaaaaatt gaaacgact tgcaattta caagacaat 
      gttttaatc gacgacagg acgaatttt acgatcggt 
      cgccggttt ctagaagca aagaaaaac ggatatcct 
      tttatcgta actttaggt aaaaaaatc gtagaaaac 
      ccactttta tacgaaatt actaattta aaaacgaat 
      gaaacgcta tttctaagt gagaatgat ttaatgaat 
      tatcttagg agtcagtta tcgtcgttt tcgagcgac 
      tctgataat aaataa
    mRNA sequence
    • >TC001328 RA
      aatttttcg aaaaatggt tattcggaa cttgcaccg 
      actctcccg ccttttcca gccggtttc ggtggtccc 
      caaaaatgc ggaagtcaa gcggcacga tatcacttc 
      gaagagtca acgattaat gctagagct tgggattat 
      aagacaagc gagtccggg ttgtttcca ttttttgcc 
      attaggggt caagtcttt ggagaaact aacaaggct 
      tgttgatga ggaaatgaa aaaaattgg ggggcaaaa 
      aattatttt tcccacgtt aactaacgc gaaactgtg 
      ggaaaaaac aggcagatt ggagactgt ttccagtga 
      gcttttcca aattaaaga ccggcatga ccagagtta 
      tattttaag ccctacaca tgaggaggc cgcgagtaa 
      tttacttgc gtcgttggc gccgatttc gtacaagca 
      cttaccttt aaaactgta tcagattac aagtaaata 
      tagggacga gatgaaacc cagatttgg gttaatgag 
      gggaaagca gtttcttat gaaagacat gtattcgtt 
      tgataccga cctcgaaac ggccaaaca cacttacga 
      tgaaatttg taaaagtta cgatcggat ttttaggag 
      aattgggat tgattttgt caaagtggt gggttgttc 
      gggacatat ggggggcga attatcgca cgaatacca 
      ctacaaagc ggacgtagg cgaagataa aattttaac 
      gtgcaacaa atgcaacta ttcggcaaa taccgaagt 
      ttcagggga ggaaaaatg ccccaattg tgggaacac 
      ttcagtcaa aattcactt agggataga agttggtca 
      cacttttct tttgggcga aaaatactc aaaaccgct 
      taatgccac ttttttgaa taatcacgg caaaccgga 
      cgtgttaca aatgggcag ttatggact ggggttatc 
      acggatttt agctgccgt aattgaagt catgtcaag 
      tgaacaaga aatcaggtg gccccagat tttatcacc 
      ttacagcgt cattatttt gcctccgaa aagcggaag 
      taaggaaga aacacacat taaggattc gggtctagt 
      tgactcact ttacgaaaa aattgaaac gacttgcaa 
      tttacaaga caatgtttt aatcgacga caggacgaa 
      ttttacgat cggtcgccg gtttctaga agcaaagaa 
      aaacggata tccttttat cgtaacttt aggtaaaaa 
      aatcgtaga aaacccact tttatacga aattactaa 
      tttaaaaac gaatgaaac gctatttct aagtgagaa 
      tgatttaat gaattatct taggagtca gttatcgtc 
      gttttcgag cgactctga taataaata acttccaga 
      aggtgattc aatgtttgc acttaacat atccggtat 
      tttcattaa cagcccggc agcgtaacg acgccacat 
      tttcccttg tcttatcct cgcactcct cgcttgctc 
      cgtttcggt ccatttagg gggcacttc cggcaaagt 
      ctcggtcac tatatccca cctaaagcc cctcaagtc 
      aaagattta aatcgtaaa atgat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY