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Gene information
General information
TC001323 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14473017..14478321
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001323
    iBeetle screen iB_00239

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001323 RA
      MTRERYHSW EPIPYDPDF NGPLRHRSC TDIICLLIF 
      FVFIGCWAG VAIYAFSSG DPNTLLVPK DSYGSRCGL 
      DEHVKDRPY LFFFDLSKC FSPSVPFTG CQTKQVCVE 
      KCPDYYYYF DQDPGNYKG HLICDYNID PSQESPEKL 
      VKEGKCARY YFKSSSFEK RCIPTIDDS FYETLEKFA 
      LNDTQKFLA SIKNIKALS QAEQVGQNI VDDLVESWW 
      KILAGVGIA VLVSIIYIM MLRWLAAPM VWLSLIGVL 
      ALLIAGVYF TTVKYIELK NKTDEDVET NNIFESYAT 
      KKDTWLVLL IICSIVLAI VLLMMIFLR KRIVLAIAL 
      VKEGSKAVS SVTSVLFFP VFPWILQIA VIAFAICVA 
      LYLATTGDP EYKIRGMTS DCPCFADFK NGTKCDPTT 
      FRNLTCLTS TLCSDVTCK FTTMNNPKW YPYLQAFNI 
      FGFFWGTFF VSALGQMIL ASVFATWYW TFNKSNLPF 
      FAVLEATGR TLRYHIGTL AFGSLIIAI CRMIRVALE 
      YIDHKLKKY DNEITKAIL CCCKCFFWC LEKFLKFLN 
      RNAYIMCAI HGKNFCMSA KDAFMLLMR NILRVFVLD 
      KVTDFLFFM GKLLITMGV GAISYVLFA TDLTGIDNS 
      GLHYEIVPV IVIMIITYI VATVFFNVY SMAVDTLFL 
      CFLEDCERN DGSSEKPYY MSRNLMKIL GKKNKQD
    • >TC001323 RB
      MTRERYHSW EPIPYDPDF NGPLRHRSC TDIICLLIF 
      FVFIGCWAG VAIYAFSSG DPNTLLVPK DSYGSRCGL 
      DEHVKDRPY LFFFDLSKC FSPSVPFTG CQTKQVCVE 
      KCPDYYYYF DQDPGNYKG HLICDYNID PSQESPEKL 
      VKEGKCARY YFKSSSFLN RCLYNTADR NKPNKDTNV 
      EKEISELKK HLSKIAMWM SLFVQNTIG YFTDNQNYQ 
      QKPTHLLLI AVEKRCIPT IDDSFYETL EKFALNDTQ 
      KFLASIKNI KALSQAEQV GQNIVDDLV ESWWKILAG 
      VGIAVLVSI IYIMMLRWL AAPMVWLSL IGVLALLIA 
      GVYFTTVKY IELKNKTDE DVETNNIFE SYATKKDTW 
      LVLLIICSI VLAIVLLMM IFLRKRIVL AIALVKEGS 
      KAVSSVTSV LFFPVFPWI LQIAVIAFA ICVALYLAT 
      TGDPEYKIR GMTSDCPCF ADFKNGTKC DPTTFRNLT 
      CLTSTLCSD VTCKFTTMN NPKWYPYLQ AFNIFGFFW 
      GTFFVSALG QMILASVFA TWYWTFNKS NLPFFAVLE 
      ATGRTLRYH IGTLAFGSL IIAICRMIR VALEYIDHK 
      LKKYDNEIT KAILCCCKC FFWCLEKFL KFLNRNAYI 
      MCAIHGKNF CMSAKDAFM LLMRNILRV FVLDKVTDF 
      LFFMGKLLI TMGVGAISY VLFATDLTG IDNSGLHYE 
      IVPVIVIMI ITYIVATVF FNVYSMAVD TLFLCFLED 
      CERNDGSSE KPYYMSRNL MKILGKKNK QD
    • >TC001323 RC
      MARSKIPEG LGEPIPYDP DFNGPLRHR SCTDIICLL 
      IFFVFIGCW AGVAIYAFS SGDPNTLLV PKDSYGSRC 
      GLDEHVKDR PYLFFFDLS KCFSPSVPF TGCQTKQVC 
      VEKCPDYYY YFDQDPGNY KGHLICDYN IDPSQESPE 
      KLVKEGKCA RYYFKSSSF LNRCLYNTA DRNKPNKDT 
      NVEKEISEL KKHLSKIAM WMSLFVQNT IGYFTDNQN 
      YQQKPTHLL LIAVEKRCI PTIDDSFYE TLEKFALND 
      TQKFLASIK NIKALSQAE QVGQNIVDD LVESWWKIL 
      AGVGIAVLV SIIYIMMLR WLAAPMVWL SLIGVLALL 
      IAGVYFTTV KYIELKNKT DEDVETNNI FESYATKKD 
      TWLVLLIIC SIVLAIVLL MMIFLRKRI VLAIALVKE 
      GSKAVSSVT SVLFFPVFP WILQIAVIA FAICVALYL 
      ATTGDPEYK IRGMTSDCP CFADFKNGT KCDPTTFRN 
      LTCLTSTLC SDVTCKFTT MNNPKWYPY LQAFNIFGF 
      FWGTFFVSA LGQMILASV FATWYWTFN KSNLPFFAV 
      LEATGRTLR YHIGTLAFG SLIIAICRM IRVALEYID 
      HKLKKYDNE ITKAILCCC KCFFWCLEK FLKFLNRNA 
      YIMCAIHGK NFCMSAKDA FMLLMRNIL RVFVLDKVT 
      DFLFFMGKL LITMGVGAI SYVLFATDL TGIDNSGLH 
      YEIVPVIVI MIITYIVAT VFFNVYSMA VDTLFLCFL 
      EDCERNDGS SEKPYYMSR NLMKILGKK NKQD
    CDS sequence
    • >TC001323 RA
      atgactcga gagaggtac cattcatgg gaaccaatt 
      ccgtacgac cccgatttc aacggccct ttgcgccac 
      cgctcttgc accgacata atctgtctt ttgattttt 
      ttcgtattt attggatgt tgggcagga gtggcaatt 
      tacgctttc agtagtgga gaccctaac actttactg 
      gtacccaaa gattcgtac ggtagtcgc tgtggtttg 
      gacgaacac gttaaagat cgtccctat ttatttttc 
      ttcgattta agcaaatgt ttctcacct tcggtacct 
      ttcacggga tgtcagacg aaacaagtt tgcgtggag 
      aaatgtcca gattattat tactatttt gatcaagac 
      ccgggtaat tataagggc catttgata tgtgactat 
      aacattgac cccagtcaa gagagtccc gagaaattg 
      gtgaaggag ggaaaatgt gcgagatat tacttcaag 
      agctcctca tttgaaaaa cggtgcata ccgaccatt 
      gacgattct ttttacgaa acactggag aaattcgct 
      ctgaatgac actcaaaag tttcttgcg agcataaaa 
      aacattaaa gctctgtct caagccgaa caggtcggc 
      caaaacatc gtcgatgat cttgtagaa tcctggtgg 
      aaaattctt gcaggtgtc ggaattgcc gttcttgta 
      tcaattatt tatatcatg atgttgaga tggctggct 
      gctcctatg gtgtggctg agtttgatt ggggttcta 
      gcactgctg attgcaggc gtttatttc acaacggtc 
      aagtacata gaactgaag aataaaacc gacgaggac 
      gttgaaact aacaatatt tttgaatct tatgctacg 
      aaaaaagac acgtggctc gttttgctc ataatttgc 
      tcgattgtg ctcgcaatt gtgctcctc atgatgatt 
      ttcttgcgg aagaggatt gtcctagcg attgcttta 
      gtcaaagaa gggagcaaa gccgtaagt tcagtcacg 
      tcggtgcta tttttccca gtcttcccg tggattctc 
      caaattgca gtcatagca tttgccatt tgtgtcgcc 
      ctgtatctt gcaaccaca ggcgatcct gaatataaa 
      attcgcgga atgacgtcc gactgtcca tgtttcgct 
      gatttcaaa aatggcaca aaatgtgac ccaacaaca 
      ttcagaaac cttacttgt ttaacttca acactttgt 
      tcggacgtc acttgcaaa ttcacaacc atgaacaac 
      cccaaatgg tatccgtac ttgcaagca ttcaacata 
      tttggcttt ttctggggc actttcttc gtctcagcc 
      ctaggccag atgatcctg gcttcagtt ttcgccaca 
      tggtactgg actttcaac aaatcaaac ttaccgttc 
      tttgcggtt ttagaagcc accggacgg accctaagg 
      taccacatc ggtactctc gcgtttggg tctttgatc 
      attgccata tgtcggatg attcgagtg gcattggag 
      tacatcgac cacaaactt aaaaaatac gataacgag 
      ataaccaaa gcgattttg tgttgttgc aaatgcttc 
      ttctggtgt ttggagaag ttcttgaag tttctgaac 
      cgaaatgcg tacatcatg tgcgcaatt cacggcaaa 
      aacttttgc atgagtgcc aaagacgcc ttcatgctc 
      ttgatgagg aacatcctg cgagtcttc gtcttggat 
      aaggtgact gattttctc ttctttatg ggaaaactc 
      ctgattacg atgggagtt ggggccatt tcctacgtc 
      ctgtttgcc accgactta acaggaatt gataattca 
      ggcttacac tacgagatt gtcccagtt atagttata 
      atgattatc acatatatt gtcgcaact gtctttttc 
      aacgtttac agcatggct gtggacacg ctcttcttg 
      tgtttcctt gaagactgc gaacggaac gatggaagc 
      agcgaaaag ccttattac atgtctcgg aatttaatg 
      aaaattttg ggcaaaaaa aataaacag gattag
    • >TC001323 RB
      atgactcga gagaggtac cattcatgg gaaccaatt 
      ccgtacgac cccgatttc aacggccct ttgcgccac 
      cgctcttgc accgacata atctgtctt ttgattttt 
      ttcgtattt attggatgt tgggcagga gtggcaatt 
      tacgctttc agtagtgga gaccctaac actttactg 
      gtacccaaa gattcgtac ggtagtcgc tgtggtttg 
      gacgaacac gttaaagat cgtccctat ttatttttc 
      ttcgattta agcaaatgt ttctcacct tcggtacct 
      ttcacggga tgtcagacg aaacaagtt tgcgtggag 
      aaatgtcca gattattat tactatttt gatcaagac 
      ccgggtaat tataagggc catttgata tgtgactat 
      aacattgac cccagtcaa gagagtccc gagaaattg 
      gtgaaggag ggaaaatgt gcgagatat tacttcaag 
      agctcctca ttcttaaac agatgtttg tacaatact 
      gctgatcgt aacaagcct aacaaggat acaaacgta 
      gaaaaagaa atttctgaa ttaaaaaaa catttgagt 
      aaaattgca atgtggatg tcgctgttt gttcaaaac 
      acgattggc tatttcaca gacaatcaa aactatcaa 
      cagaagcca acacattta ttgcttatt gcagttgaa 
      aaacggtgc ataccgacc attgacgat tctttttac 
      gaaacactg gagaaattc gctctgaat gacactcaa 
      aagtttctt gcgagcata aaaaacatt aaagctctg 
      tctcaagcc gaacaggtc ggccaaaac atcgtcgat 
      gatcttgta gaatcctgg tggaaaatt cttgcaggt 
      gtcggaatt gccgttctt gtatcaatt atttatatc 
      atgatgttg agatggctg gctgctcct atggtgtgg 
      ctgagtttg attggggtt ctagcactg ctgattgca 
      ggcgtttat ttcacaacg gtcaagtac atagaactg 
      aagaataaa accgacgag gacgttgaa actaacaat 
      atttttgaa tcttatgct acgaaaaaa gacacgtgg 
      ctcgttttg ctcataatt tgctcgatt gtgctcgca 
      attgtgctc ctcatgatg attttcttg cggaagagg 
      attgtccta gcgattgct ttagtcaaa gaagggagc 
      aaagccgta agttcagtc acgtcggtg ctatttttc 
      ccagtcttc ccgtggatt ctccaaatt gcagtcata 
      gcatttgcc atttgtgtc gccctgtat cttgcaacc 
      acaggcgat cctgaatat aaaattcgc ggaatgacg 
      tccgactgt ccatgtttc gctgatttc aaaaatggc 
      acaaaatgt gacccaaca acattcaga aaccttact 
      tgtttaact tcaacactt tgttcggac gtcacttgc 
      aaattcaca accatgaac aaccccaaa tggtatccg 
      tacttgcaa gcattcaac atatttggc tttttctgg 
      ggcactttc ttcgtctca gccctaggc cagatgatc 
      ctggcttca gttttcgcc acatggtac tggactttc 
      aacaaatca aacttaccg ttctttgcg gttttagaa 
      gccaccgga cggacccta aggtaccac atcggtact 
      ctcgcgttt gggtctttg atcattgcc atatgtcgg 
      atgattcga gtggcattg gagtacatc gaccacaaa 
      cttaaaaaa tacgataac gagataacc aaagcgatt 
      ttgtgttgt tgcaaatgc ttcttctgg tgtttggag 
      aagttcttg aagtttctg aaccgaaat gcgtacatc 
      atgtgcgca attcacggc aaaaacttt tgcatgagt 
      gccaaagac gccttcatg ctcttgatg aggaacatc 
      ctgcgagtc ttcgtcttg gataaggtg actgatttt 
      ctcttcttt atgggaaaa ctcctgatt acgatggga 
      gttggggcc atttcctac gtcctgttt gccaccgac 
      ttaacagga attgataat tcaggctta cactacgag 
      attgtccca gttatagtt ataatgatt atcacatat 
      attgtcgca actgtcttt ttcaacgtt tacagcatg 
      gctgtggac acgctcttc ttgtgtttc cttgaagac 
      tgcgaacgg aacgatgga agcagcgaa aagccttat 
      tacatgtct cggaattta atgaaaatt ttgggcaaa 
      aaaaataaa caggattag
    • >TC001323 RC
      atggcaagg tcgaagatt ccagagggt cttggggaa 
      ccaattccg tacgacccc gatttcaac ggccctttg 
      cgccaccgc tcttgcacc gacataatc tgtcttttg 
      atttttttc gtatttatt ggatgttgg gcaggagtg 
      gcaatttac gctttcagt agtggagac cctaacact 
      ttactggta cccaaagat tcgtacggt agtcgctgt 
      ggtttggac gaacacgtt aaagatcgt ccctattta 
      tttttcttc gatttaagc aaatgtttc tcaccttcg 
      gtacctttc acgggatgt cagacgaaa caagtttgc 
      gtggagaaa tgtccagat tattattac tattttgat 
      caagacccg ggtaattat aagggccat ttgatatgt 
      gactataac attgacccc agtcaagag agtcccgag 
      aaattggtg aaggaggga aaatgtgcg agatattac 
      ttcaagagc tcctcattc ttaaacaga tgtttgtac 
      aatactgct gatcgtaac aagcctaac aaggataca 
      aacgtagaa aaagaaatt tctgaatta aaaaaacat 
      ttgagtaaa attgcaatg tggatgtcg ctgtttgtt 
      caaaacacg attggctat ttcacagac aatcaaaac 
      tatcaacag aagccaaca catttattg cttattgca 
      gttgaaaaa cggtgcata ccgaccatt gacgattct 
      ttttacgaa acactggag aaattcgct ctgaatgac 
      actcaaaag tttcttgcg agcataaaa aacattaaa 
      gctctgtct caagccgaa caggtcggc caaaacatc 
      gtcgatgat cttgtagaa tcctggtgg aaaattctt 
      gcaggtgtc ggaattgcc gttcttgta tcaattatt 
      tatatcatg atgttgaga tggctggct gctcctatg 
      gtgtggctg agtttgatt ggggttcta gcactgctg 
      attgcaggc gtttatttc acaacggtc aagtacata 
      gaactgaag aataaaacc gacgaggac gttgaaact 
      aacaatatt tttgaatct tatgctacg aaaaaagac 
      acgtggctc gttttgctc ataatttgc tcgattgtg 
      ctcgcaatt gtgctcctc atgatgatt ttcttgcgg 
      aagaggatt gtcctagcg attgcttta gtcaaagaa 
      gggagcaaa gccgtaagt tcagtcacg tcggtgcta 
      tttttccca gtcttcccg tggattctc caaattgca 
      gtcatagca tttgccatt tgtgtcgcc ctgtatctt 
      gcaaccaca ggcgatcct gaatataaa attcgcgga 
      atgacgtcc gactgtcca tgtttcgct gatttcaaa 
      aatggcaca aaatgtgac ccaacaaca ttcagaaac 
      cttacttgt ttaacttca acactttgt tcggacgtc 
      acttgcaaa ttcacaacc atgaacaac cccaaatgg 
      tatccgtac ttgcaagca ttcaacata tttggcttt 
      ttctggggc actttcttc gtctcagcc ctaggccag 
      atgatcctg gcttcagtt ttcgccaca tggtactgg 
      actttcaac aaatcaaac ttaccgttc tttgcggtt 
      ttagaagcc accggacgg accctaagg taccacatc 
      ggtactctc gcgtttggg tctttgatc attgccata 
      tgtcggatg attcgagtg gcattggag tacatcgac 
      cacaaactt aaaaaatac gataacgag ataaccaaa 
      gcgattttg tgttgttgc aaatgcttc ttctggtgt 
      ttggagaag ttcttgaag tttctgaac cgaaatgcg 
      tacatcatg tgcgcaatt cacggcaaa aacttttgc 
      atgagtgcc aaagacgcc ttcatgctc ttgatgagg 
      aacatcctg cgagtcttc gtcttggat aaggtgact 
      gattttctc ttctttatg ggaaaactc ctgattacg 
      atgggagtt ggggccatt tcctacgtc ctgtttgcc 
      accgactta acaggaatt gataattca ggcttacac 
      tacgagatt gtcccagtt atagttata atgattatc 
      acatatatt gtcgcaact gtctttttc aacgtttac 
      agcatggct gtggacacg ctcttcttg tgtttcctt 
      gaagactgc gaacggaac gatggaagc agcgaaaag 
      ccttattac atgtctcgg aatttaatg aaaattttg 
      ggcaaaaaa aataaacag gattag
    mRNA sequence
    • >TC001323 RA
      cccgaatta ttaagtgac atttaattc catttgaac 
      cggacaata aacttttaa ccgcctatt gttagttga 
      aaagtgaaa acccaatct acttttaga aatctgctt 
      gcacaagtg ggacgtgct agttgcggt ttttgaaga 
      ctgttgact cataaataa attctggct cttttgtta 
      tcattattt aaacaaagg ctaaattac accaaaatg 
      tagtaattg gtttgttgt gattgttaa ttgcgagtc 
      aaggttgta gcagattat ttattgttc ggcttcatt 
      ctcttgtgc gggagagtg ttgtaaaca aacaagtgg 
      ttacaatac attgcattt tcttccacg gtgtgttta 
      gttaattgg aaggttgtt gtcgcttgt actgtaatt 
      gcagtgtta gtgcgacga tgtttttct gaagctgag 
      tgggtatga aagacggca ggaaattac aaaaattaa 
      ttcaatgtc agtgagacg atgacgctc cacatctaa 
      ttttttaaa caaagttcg agaattgta tggttttag 
      attgagaag ttgtgcatg gttatagga tggcaaggt 
      cgaagattc cagagggtc ttggtaagt agaacatta 
      caatcgagc ttaaaatat ttgcaagga aaatttttt 
      taacatgtg ttggaattg tttggtaaa aagtcttca 
      aattttata aaaagaaat tgaaacatt tttgactaa 
      tgggtattt cctctggta tttttattt cgctttaga 
      acgattgat gcaaacgag gacagtgat gagaaaaaa 
      gtaataaaa gtcgtgact ggactgaaa aattgctaa 
      ataagtaaa tatttagcg ttttatgtg ttttagtta 
      caaagcggt cttttactg tgttgtagc ttgtagctc 
      ttataatga acgaattaa aaaagaaac aaaaccggg 
      aaaaattcg ggcaaaaaa ctgagtaat aataaaatt 
      gcaacatcg tataattta agaacactt ccttgtttt 
      gttctgtgc aaccttgca ttcattact taaaaatag 
      tgaagaatt atccatttt aataacact gttacgtaa 
      acaacttct ggaaatctg gaaactcct catcgatac 
      tttgcagaa ttttcgata agaatctgt tcaaacaaa 
      aagtatgaa gaaatcttt ttccgtgtc aagataatt 
      tctagtgtc aacagcaat tactcgcgt ttaaccttg 
      tagttacat ttttgtgtc agtttatgt ttattcctt 
      cagtagttg tgtgtattg aaaatgact cgagagagg 
      taccattca tgggaacca attccgtac gaccccgat 
      ttcaacggc cctttgcgc caccgctct tgcaccgac 
      ataatctgt cttttgatt tttttcgta tttattgga 
      tgttgggca ggagtggca atttacgct ttcagtagt 
      ggagaccct aacacttta ctggtaccc aaagattcg 
      tacggtagt cgctgtggt ttggacgaa cacgttaaa 
      gatcgtccc tatttattt ttcttcgat ttaagcaaa 
      tgtttctca ccttcggta cctttcacg ggatgtcag 
      acgaaacaa gtttgcgtg gagaaatgt ccagattat 
      tattactat tttgatcaa gacccgggt aattataag 
      ggccatttg atatgtgac tataacatt gaccccagt 
      caagagagt cccgagaaa ttggtgaag gagggaaaa 
      tgtgcgaga tattacttc aagagctcc tcatttgaa 
      aaacggtgc ataccgacc attgacgat tctttttac 
      gaaacactg gagaaattc gctctgaat gacactcaa 
      aagtttctt gcgagcata aaaaacatt aaagctctg 
      tctcaagcc gaacaggtc ggccaaaac atcgtcgat 
      gatcttgta gaatcctgg tggaaaatt cttgcaggt 
      gtcggaatt gccgttctt gtatcaatt atttatatc 
      atgatgttg agatggctg gctgctcct atggtgtgg 
      ctgagtttg attggggtt ctagcactg ctgattgca 
      ggcgtttat ttcacaacg gtcaagtac atagaactg 
      aagaataaa accgacgag gacgttgaa actaacaat 
      atttttgaa tcttatgct acgaaaaaa gacacgtgg 
      ctcgttttg ctcataatt tgctcgatt gtgctcgca 
      attgtgctc ctcatgatg attttcttg cggaagagg 
      attgtccta gcgattgct ttagtcaaa gaagggagc 
      aaagccgta agttcagtc acgtcggtg ctatttttc 
      ccagtcttc ccgtggatt ctccaaatt gcagtcata 
      gcatttgcc atttgtgtc gccctgtat cttgcaacc 
      acaggcgat cctgaatat aaaattcgc ggaatgacg 
      tccgactgt ccatgtttc gctgatttc aaaaatggc 
      acaaaatgt gacccaaca acattcaga aaccttact 
      tgtttaact tcaacactt tgttcggac gtcacttgc 
      aaattcaca accatgaac aaccccaaa tggtatccg 
      tacttgcaa gcattcaac atatttggc tttttctgg 
      ggcactttc ttcgtctca gccctaggc cagatgatc 
      ctggcttca gttttcgcc acatggtac tggactttc 
      aacaaatca aacttaccg ttctttgcg gttttagaa 
      gccaccgga cggacccta aggtaccac atcggtact 
      ctcgcgttt gggtctttg atcattgcc atatgtcgg 
      atgattcga gtggcattg gagtacatc gaccacaaa 
      cttaaaaaa tacgataac gagataacc aaagcgatt 
      ttgtgttgt tgcaaatgc ttcttctgg tgtttggag 
      aagttcttg aagtttctg aaccgaaat gcgtacatc 
      atgtgcgca attcacggc aaaaacttt tgcatgagt 
      gccaaagac gccttcatg ctcttgatg aggaacatc 
      ctgcgagtc ttcgtcttg gataaggtg actgatttt 
      ctcttcttt atgggaaaa ctcctgatt acgatggga 
      gttggggcc atttcctac gtcctgttt gccaccgac 
      ttaacagga attgataat tcaggctta cactacgag 
      attgtccca gttatagtt ataatgatt atcacatat 
      attgtcgca actgtcttt ttcaacgtt tacagcatg 
      gctgtggac acgctcttc ttgtgtttc cttgaagac 
      tgcgaacgg aacgatgga agcagcgaa aagccttat 
      tacatgtct cggaattta atgaaaatt ttgggcaaa 
      aaaaataaa caggattag aactagtta gtggacgca 
      catgtattc tgtga
    • >TC001323 RB
      cccgaatta ttaagtgac atttaattc catttgaac 
      cggacaata aacttttaa ccgcctatt gttagttga 
      aaagtgaaa acccaatct acttttaga aatctgctt 
      gcacaagtg ggacgtgct agttgcggt ttttgaaga 
      ctgttgact cataaataa attctggct cttttgtta 
      tcattattt aaacaaagg ctaaattac accaaaatg 
      tagtaattg gtttgttgt gattgttaa ttgcgagtc 
      aaggttgta gcagattat ttattgttc ggcttcatt 
      ctcttgtgc gggagagtg ttgtaaaca aacaagtgg 
      ttacaatac attgcattt tcttccacg gtgtgttta 
      gttaattgg aaggttgtt gtcgcttgt actgtaatt 
      gcagtgtta gtgcgacga tgtttttct gaagctgag 
      tgggtatga aagacggca ggaaattac aaaaattaa 
      ttcaatgtc agtgagacg atgacgctc cacatctaa 
      ttttttaaa caaagttcg agaattgta tggttttag 
      attgagaag ttgtgcatg gttatagga tggcaaggt 
      cgaagattc cagagggtc ttggtaagt agaacatta 
      caatcgagc ttaaaatat ttgcaagga aaatttttt 
      taacatgtg ttggaattg tttggtaaa aagtcttca 
      aattttata aaaagaaat tgaaacatt tttgactaa 
      tgggtattt cctctggta tttttattt cgctttaga 
      acgattgat gcaaacgag gacagtgat gagaaaaaa 
      gtaataaaa gtcgtgact ggactgaaa aattgctaa 
      ataagtaaa tatttagcg ttttatgtg ttttagtta 
      caaagcggt cttttactg tgttgtagc ttgtagctc 
      ttataatga acgaattaa aaaagaaac aaaaccggg 
      aaaaattcg ggcaaaaaa ctgagtaat aataaaatt 
      gcaacatcg tataattta agaacactt ccttgtttt 
      gttctgtgc aaccttgca ttcattact taaaaatag 
      tgaagaatt atccatttt aataacact gttacgtaa 
      acaacttct ggaaatctg gaaactcct catcgatac 
      tttgcagaa ttttcgata agaatctgt tcaaacaaa 
      aagtatgaa gaaatcttt ttccgtgtc aagataatt 
      tctagtgtc aacagcaat tactcgcgt ttaaccttg 
      tagttacat ttttgtgtc agtttatgt ttattcctt 
      cagtagttg tgtgtattg aaaatgact cgagagagg 
      taccattca tgggaacca attccgtac gaccccgat 
      ttcaacggc cctttgcgc caccgctct tgcaccgac 
      ataatctgt cttttgatt tttttcgta tttattgga 
      tgttgggca ggagtggca atttacgct ttcagtagt 
      ggagaccct aacacttta ctggtaccc aaagattcg 
      tacggtagt cgctgtggt ttggacgaa cacgttaaa 
      gatcgtccc tatttattt ttcttcgat ttaagcaaa 
      tgtttctca ccttcggta cctttcacg ggatgtcag 
      acgaaacaa gtttgcgtg gagaaatgt ccagattat 
      tattactat tttgatcaa gacccgggt aattataag 
      ggccatttg atatgtgac tataacatt gaccccagt 
      caagagagt cccgagaaa ttggtgaag gagggaaaa 
      tgtgcgaga tattacttc aagagctcc tcattctta 
      aacagatgt ttgtacaat actgctgat cgtaacaag 
      cctaacaag gatacaaac gtagaaaaa gaaatttct 
      gaattaaaa aaacatttg agtaaaatt gcaatgtgg 
      atgtcgctg tttgttcaa aacacgatt ggctatttc 
      acagacaat caaaactat caacagaag ccaacacat 
      ttattgctt attgcagtt gaaaaacgg tgcataccg 
      accattgac gattctttt tacgaaaca ctggagaaa 
      ttcgctctg aatgacact caaaagttt cttgcgagc 
      ataaaaaac attaaagct ctgtctcaa gccgaacag 
      gtcggccaa aacatcgtc gatgatctt gtagaatcc 
      tggtggaaa attcttgca ggtgtcgga attgccgtt 
      cttgtatca attatttat atcatgatg ttgagatgg 
      ctggctgct cctatggtg tggctgagt ttgattggg 
      gttctagca ctgctgatt gcaggcgtt tatttcaca 
      acggtcaag tacatagaa ctgaagaat aaaaccgac 
      gaggacgtt gaaactaac aatattttt gaatcttat 
      gctacgaaa aaagacacg tggctcgtt ttgctcata 
      atttgctcg attgtgctc gcaattgtg ctcctcatg 
      atgattttc ttgcggaag aggattgtc ctagcgatt 
      gctttagtc aaagaaggg agcaaagcc gtaagttca 
      gtcacgtcg gtgctattt ttcccagtc ttcccgtgg 
      attctccaa attgcagtc atagcattt gccatttgt 
      gtcgccctg tatcttgca accacaggc gatcctgaa 
      tataaaatt cgcggaatg acgtccgac tgtccatgt 
      ttcgctgat ttcaaaaat ggcacaaaa tgtgaccca 
      acaacattc agaaacctt acttgttta acttcaaca 
      ctttgttcg gacgtcact tgcaaattc acaaccatg 
      aacaacccc aaatggtat ccgtacttg caagcattc 
      aacatattt ggctttttc tggggcact ttcttcgtc 
      tcagcccta ggccagatg atcctggct tcagttttc 
      gccacatgg tactggact ttcaacaaa tcaaactta 
      ccgttcttt gcggtttta gaagccacc ggacggacc 
      ctaaggtac cacatcggt actctcgcg tttgggtct 
      ttgatcatt gccatatgt cggatgatt cgagtggca 
      ttggagtac atcgaccac aaacttaaa aaatacgat 
      aacgagata accaaagcg attttgtgt tgttgcaaa 
      tgcttcttc tggtgtttg gagaagttc ttgaagttt 
      ctgaaccga aatgcgtac atcatgtgc gcaattcac 
      ggcaaaaac ttttgcatg agtgccaaa gacgccttc 
      atgctcttg atgaggaac atcctgcga gtcttcgtc 
      ttggataag gtgactgat tttctcttc tttatggga 
      aaactcctg attacgatg ggagttggg gccatttcc 
      tacgtcctg tttgccacc gacttaaca ggaattgat 
      aattcaggc ttacactac gagattgtc ccagttata 
      gttataatg attatcaca tatattgtc gcaactgtc 
      tttttcaac gtttacagc atggctgtg gacacgctc 
      ttcttgtgt ttccttgaa gactgcgaa cggaacgat 
      ggaagcagc gaaaagcct tattacatg tctcggaat 
      ttaatgaaa attttgggc aaaaaaaat aaacaggat 
      tagaactag ttagtggac gcacatgta ttctgtga
    • >TC001323 RC
      cccgaatta ttaagtgac atttaattc catttgaac 
      cggacaata aacttttaa ccgcctatt gttagttga 
      aaagtgaaa acccaatct acttttaga aatctgctt 
      gcacaagtg ggacgtgct agttgcggt ttttgaaga 
      ctgttgact cataaataa attctggct cttttgtta 
      tcattattt aaacaaagg ctaaattac accaaaatg 
      tagtaattg gtttgttgt gattgttaa ttgcgagtc 
      aaggttgta gcagattat ttattgttc ggcttcatt 
      ctcttgtgc gggagagtg ttgtaaaca aacaagtgg 
      ttacaatac attgcattt tcttccacg gtgtgttta 
      gttaattgg aaggttgtt gtcgcttgt actgtaatt 
      gcagtgtta gtgcgacga tgtttttct gaagctgag 
      tgggtatga aagacggca ggaaattac aaaaattaa 
      ttcaatgtc agtgagacg atgacgctc cacatctaa 
      ttttttaaa caaagttcg agattgaga agttgtgca 
      tggttatag gatggcaag gtcgaagat tccagaggg 
      tcttgggga accaattcc gtacgaccc cgatttcaa 
      cggcccttt gcgccaccg ctcttgcac cgacataat 
      ctgtctttt gattttttt cgtatttat tggatgttg 
      ggcaggagt ggcaattta cgctttcag tagtggaga 
      ccctaacac tttactggt acccaaaga ttcgtacgg 
      tagtcgctg tggtttgga cgaacacgt taaagatcg 
      tccctattt atttttctt cgatttaag caaatgttt 
      ctcaccttc ggtaccttt cacgggatg tcagacgaa 
      acaagtttg cgtggagaa atgtccaga ttattatta 
      ctattttga tcaagaccc gggtaatta taagggcca 
      tttgatatg tgactataa cattgaccc cagtcaaga 
      gagtcccga gaaattggt gaaggaggg aaaatgtgc 
      gagatatta cttcaagag ctcctcatt cttaaacag 
      atgtttgta caatactgc tgatcgtaa caagcctaa 
      caaggatac aaacgtaga aaaagaaat ttctgaatt 
      aaaaaaaca tttgagtaa aattgcaat gtggatgtc 
      gctgtttgt tcaaaacac gattggcta tttcacaga 
      caatcaaaa ctatcaaca gaagccaac acatttatt 
      gcttattgc agttgaaaa acggtgcat accgaccat 
      tgacgattc tttttacga aacactgga gaaattcgc 
      tctgaatga cactcaaaa gtttcttgc gagcataaa 
      aaacattaa agctctgtc tcaagccga acaggtcgg 
      ccaaaacat cgtcgatga tcttgtaga atcctggtg 
      gaaaattct tgcaggtgt cggaattgc cgttcttgt 
      atcaattat ttatatcat gatgttgag atggctggc 
      tgctcctat ggtgtggct gagtttgat tggggttct 
      agcactgct gattgcagg cgtttattt cacaacggt 
      caagtacat agaactgaa gaataaaac cgacgagga 
      cgttgaaac taacaatat ttttgaatc ttatgctac 
      gaaaaaaga cacgtggct cgttttgct cataatttg 
      ctcgattgt gctcgcaat tgtgctcct catgatgat 
      tttcttgcg gaagaggat tgtcctagc gattgcttt 
      agtcaaaga agggagcaa agccgtaag ttcagtcac 
      gtcggtgct atttttccc agtcttccc gtggattct 
      ccaaattgc agtcatagc atttgccat ttgtgtcgc 
      cctgtatct tgcaaccac aggcgatcc tgaatataa 
      aattcgcgg aatgacgtc cgactgtcc atgtttcgc 
      tgatttcaa aaatggcac aaaatgtga cccaacaac 
      attcagaaa ccttacttg tttaacttc aacactttg 
      ttcggacgt cacttgcaa attcacaac catgaacaa 
      ccccaaatg gtatccgta cttgcaagc attcaacat 
      atttggctt tttctgggg cactttctt cgtctcagc 
      cctaggcca gatgatcct ggcttcagt tttcgccac 
      atggtactg gactttcaa caaatcaaa cttaccgtt 
      ctttgcggt tttagaagc caccggacg gaccctaag 
      gtaccacat cggtactct cgcgtttgg gtctttgat 
      cattgccat atgtcggat gattcgagt ggcattgga 
      gtacatcga ccacaaact taaaaaata cgataacga 
      gataaccaa agcgatttt gtgttgttg caaatgctt 
      cttctggtg tttggagaa gttcttgaa gtttctgaa 
      ccgaaatgc gtacatcat gtgcgcaat tcacggcaa 
      aaacttttg catgagtgc caaagacgc cttcatgct 
      cttgatgag gaacatcct gcgagtctt cgtcttgga 
      taaggtgac tgattttct cttctttat gggaaaact 
      cctgattac gatgggagt tggggccat ttcctacgt 
      cctgtttgc caccgactt aacaggaat tgataattc 
      aggcttaca ctacgagat tgtcccagt tatagttat 
      aatgattat cacatatat tgtcgcaac tgtcttttt 
      caacgttta cagcatggc tgtggacac gctcttctt 
      gtgtttcct tgaagactg cgaacggaa cgatggaag 
      cagcgaaaa gccttatta catgtctcg gaatttaat 
      gaaaatttt gggcaaaaa aaataaaca ggattagaa 
      ctagttagt ggacgcaca tgtattctg tga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY