General information | |||
TC001292 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 14016336..14019511 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC001292 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00237 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 6 (11 dpi)
not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC001292 RA
MPTNVESPP CKKSNVEVM TPHRLTVAI LIRDYCLFR DSDRFSCED DGVLKARYR RDFCVLILK LLQSPDLSL EELRNILVS TKYEVHQSV IEAFDETLA SLYTNGTGT MLDIIDSFN RIMTFNENI AAGIEPNCF VSKSSIVGY YLRRLIVNF DKLTFSEVT SVYLTFKRY YEDWQKSLT TRDQIAIEV NKVICPDDW SANRNQWSR RQAELFIAT QAALLTNNE EKALPPQEL HNRISNLLQ SNRSLAEAH FLSYLNYLR VKEFCGAID SLYHCFDRS TLTDTKISS EDKSKVYRF AALNLAILH HHFGHKEEA LASLREAIK SSQVANDNV CLQHALCWL YRLTPVNKE KLLEHSVAK SFELNLSYT ASLGIQTFV QYACLISGH PHQIFETLT KGDLINCQH ILRDLISNS YAVKSALWQ FYGKTEMSS LWSQLLFYL NIDNGRSND GKAVYGEGF CLAICNVAN HLLMQGNYN LVNTVLNFA KDRFPNEPN SHIWMLCEN LFIFVRALH HENWSEAEA AAQKILVVD KWEGYLRLA ELYYYKQDY TEAHRFVDI LINRFHSDN KYKLKIFYY VRAKILLAE IQFSSCYPD KVSPEIMTL LSNCLLDTD KHNLDYHSA LIHLHIANI QLSLGMTSQ ALKILDRCL VQILAHGGN FDRARAVLL YVKCLVAHS AQLEATERR EIILNAAQM LEKVKEDFR AVEAYSRVK DVLYLQAQL YNSVDLRSE RNRCALEFR QLDEERTTK NYYTLVRFL
- >TC001292 RA
atgcccaca aacgtggaa agtccacct tgtaaaaaa tccaacgtt gaagtaatg actccccat agactaacc gttgcaatt ttaatccgt gattattgc ttatttcgg gattcagac cggtttagc tgtgaagat gatggggtg cttaaggcc agatacaga cgagacttt tgcgtttta atattaaag ttattgcaa agtcctgat cttagttta gaagagtta cggaacatt ttagtctca actaaatat gaagtgcac cagagcgtt attgaggct tttgatgag acactagcc agtttatat acaaacgga acaggaact atgcttgat attattgat agttttaac aggatcatg actttcaat gaaaacata gcggcagga atagaacca aactgtttt gtaagcaag agtagcatt gttggttac tatttacga agacttatc gtaaacttt gataaactc actttttcg gaagttacg agtgtttat ttaactttt aaaaggtac tatgaagat tggcaaaaa agtttaaca acacgtgac cagattgca atagaagtt aataaagtt atttgtccc gatgattgg agtgctaac agaaatcag tggtcaagg cgtcaagct gagttattt atagcgact caagccgcc cttttaacc aataatgaa gaaaaagca ttgccacct caagaactt cacaataga atctccaat ttgctccaa tcaaaccga tctttggca gaagctcac tttttgtca tacttaaat tacttaaga gtgaaggaa ttttgcggc gccattgat agtttatat cactgtttc gacagaagc actttaacc gataccaaa atctcatcg gaggataaa agtaaagtt tacagattt gcggcactc aatctagca attttgcac caccatttc ggccacaag gaagaagct ctcgcctca cttagagag gcaataaaa agctcccaa gttgcaaac gacaacgtt tgcctccaa cacgccctc tgttggctc taccgcctg accccagtc aacaaagaa aaattactc gaacactct gtagccaaa agtttcgaa ctcaaccta tcctacacc gcatcactc ggtattcaa actttcgtc caatacgcg tgtcttatc tcaggccat ccgcaccaa attttcgaa acgttaaca aaaggcgat ttgattaat tgccaacac attttgcgc gatcttatt tcgaacagt tatgcggtc aaatcagcc ctttggcag ttttacggc aagacggaa atgtcgtca ctttggagc cagttattg ttttattta aatattgat aatggacgt agtaatgac gggaaggcg gtctatggt gaaggtttc tgtttggcg atttgtaac gtggccaat caccttcta atgcaagga aattacaat ttggtaaac actgtgttg aattttgcg aaagatcgg tttccaaac gagccaaat tcgcacatt tggatgttg tgcgaaaat ttatttatt ttcgtgcga gcgttacat catgaaaat tggagcgaa gctgaagct gcagctcag aaaattcta gtcgtggat aagtgggag ggttacttg agactagct gagttgtac tactacaag caggattac actgaagcg caccgattt gttgatatt ttgataaat cgttttcac agcgataac aaatacaaa cttaaaatt ttctattac gtgagggcc aaaattttg ctcgcggaa atccaattt tccagttgt tatcccgat aaagtctca ccggaaatt atgactctt cttagcaat tgtctccta gacactgat aaacacaat ttggattat cattccgct ttgattcac ttgcacatt gccaatatt cagttaagt ttgggaatg actagtcag gccctaaaa attttggac cggtgtctt gttcaaatt ctggcacat gggggcaat ttcgataga gctagggcc gttttgttg tacgttaaa tgtctggtg gcgcattct gcgcaactt gaagccact gaaagacgg gagattatt ttaaacgct gcgcaaatg ttggagaaa gtgaaggag gattttagg gctgtggag gcttattcc agggtcaaa gacgtgtta tatttgcag gcgcaactt tacaatagc gttgatttg cggagtgag agaaacaga tgcgctttg gaatttcgg caattggat gaagaaaga acaacgaaa aattattat accctagtt aggtttttg taa
- >TC001292 RA
taaaaatgc ccacaaacg tggaaagtc caccttgta aaaaatcca acgttgaag taatgactc cccatagac taaccgttg caattttaa tccgtgatt attgcttat ttcgggatt cagaccggt ttagctgtg aagatgatg gggtgctta aggccagat acagacgag acttttgcg ttttaatat taaagttat tgcaaagtc ctgatctta gtttagaag agttacgga acattttag tctcaacta aatatgaag tgcaccaga gcgttattg aggcttttg atgagacac tagccagtt tatatacaa acggaacag gaactatgc ttgatatta ttgatagtt ttaacagga tcatgactt tcaatgaaa acatagcgg caggaatag aaccaaact gttttgtaa gcaagagta gcattgttg gttactatt tacgaagac ttatcgtaa actttgata aactcactt tttcggaag ttacgagtg tttatttaa cttttaaaa ggtactatg aagattggc aaaaaagtt taacaacac gtgaccaga ttgcaatag aagttaata aagttattt gtcccgatg attggagtg ctaacagaa atcagtggt caaggcgtc aagctgagt tatttatag cgactcaag ccgcccttt taaccaata atgaagaaa aagcattgc cacctcaag aacttcaca atagaatct ccaatttgc tccaatcaa accgatctt tggcagaag ctcactttt tgtcatact taaattact taagagtga aggaatttt gcggcgcca ttgatagtt tatatcact gtttcgaca gaagcactt taaccgata ccaaaatct catcggagg ataaaagta aagtttaca gatttgcgg cactcaatc tagcaattt tgcaccacc atttcggcc acaaggaag aagctctcg cctcactta gagaggcaa taaaaagct cccaagttg caaacgaca acgtttgcc tccaacacg ccctctgtt ggctctacc gcctgaccc cagtcaaca aagaaaaat tactcgaac actctgtag ccaaaagtt tcgaactca acctatcct acaccgcat cactcggta ttcaaactt tcgtccaat acgcgtgtc ttatctcag gccatccgc accaaattt tcgaaacgt taacaaaag gcgatttga ttaattgcc aacacattt tgcgcgatc ttatttcga acagttatg cggtcaaat cagcccttt ggcagtttt acggcaaga cggaaatgt cgtcacttt ggagccagt tattgtttt atttaaata ttgataatg gacgtagta atgacggga aggcggtct atggtgaag gtttctgtt tggcgattt gtaacgtgg ccaatcacc ttctaatgc aaggaaatt acaatttgg taaacactg tgttgaatt ttgcgaaag atcggtttc caaacgagc caaattcgc acatttgga tgttgtgcg aaaatttat ttattttcg tgcgagcgt tacatcatg aaaattgga gcgaagctg aagctgcag ctcagaaaa ttctagtcg tggataagt gggagggtt acttgagac tagctgagt tgtactact acaagcagg attacactg aagcgcacc gatttgttg atattttga taaatcgtt ttcacagcg ataacaaat acaaactta aaattttct attacgtga gggccaaaa ttttgctcg cggaaatcc aattttcca gttgttatc ccgataaag tctcaccgg aaattatga ctcttctta gcaattgtc tcctagaca ctgataaac acaatttgg attatcatt ccgctttga ttcacttgc acattgcca atattcagt taagtttgg gaatgacta gtcaggccc taaaaattt tggaccggt gtcttgttc aaattctgg cacatgggg gcaatttcg atagagcta gggccgttt tgttgtacg ttaaatgtc tggtggcgc attctgcgc aacttgaag ccactgaaa gacgggaga ttattttaa acgctgcgc aaatgttgg agaaagtga aggaggatt ttagggctg tggaggctt attccaggg tcaaagacg tgttatatt tgcaggcgc aactttaca atagcgttg atttgcgga gtgagagaa acagatgcg ctttggaat ttcggcaat tggatgaag aaagaacaa cgaaaaatt attataccc tagttaggt ttttgtaat ttgtgaaat aaacgcatt aattaaga
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
