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Gene information
General information
TC001292 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid9: 39551..42726
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001292
    iBeetle screen iB_00237
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection



    not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001292 RA
      MPTNVESPP CKKSNVEVM TPHRLTVAI LIRDYCLFR 
      DSDRFSCED DGVLKARYR RDFCVLILK LLQSPDLSL 
      EELRNILVS TKYEVHQSV IEAFDETLA SLYTNGTGT 
      MLDIIDSFN RIMTFNENI AAGIEPNCF VSKSSIVGY 
      YLRRLIVNF DKLTFSEVT SVYLTFKRY YEDWQKSLT 
      TRDQIAIEV NKVICPDDW SANRNQWSR RQAELFIAT 
      QAALLTNNE EKALPPQEL HNRISNLLQ SNRSLAEAH 
      FLSYLNYLR VKEFCGAID SLYHCFDRS TLTDTKISS 
      EDKSKVYRF AALNLAILH HHFGHKEEA LASLREAIK 
      SSQVANDNV CLQHALCWL YRLTPVNKE KLLEHSVAK 
      SFELNLSYT ASLGIQTFV QYACLISGH PHQIFETLT 
      KGDLINCQH ILRDLISNS YAVKSALWQ FYGKTEMSS 
      LWSQLLFYL NIDNGRSND GKAVYGEGF CLAICNVAN 
      HLLMQGNYN LVNTVLNFA KDRFPNEPN SHIWMLCEN 
      LFIFVRALH HENWSEAEA AAQKILVVD KWEGYLRLA 
      ELYYYKQDY TEAHRFVDI LINRFHSDN KYKLKIFYY 
      VRAKILLAE IQFSSCYPD KVSPEIMTL LSNCLLDTD 
      KHNLDYHSA LIHLHIANI QLSLGMTSQ ALKILDRCL 
      VQILAHGGN FDRARAVLL YVKCLVAHS AQLEATERR 
      EIILNAAQM LEKVKEDFR AVEAYSRVK DVLYLQAQL 
      YNSVDLRSE RNRCALEFR QLDEERTTK NYYTLVRFL
    CDS sequence
    • >TC001292 RA
      atgcccaca aacgtggaa agtccacct tgtaaaaaa 
      tccaacgtt gaagtaatg actccccat agactaacc 
      gttgcaatt ttaatccgt gattattgc ttatttcgg 
      gattcagac cggtttagc tgtgaagat gatggggtg 
      cttaaggcc agatacaga cgagacttt tgcgtttta 
      atattaaag ttattgcaa agtcctgat cttagttta 
      gaagagtta cggaacatt ttagtctca actaaatat 
      gaagtgcac cagagcgtt attgaggct tttgatgag 
      acactagcc agtttatat acaaacgga acaggaact 
      atgcttgat attattgat agttttaac aggatcatg 
      actttcaat gaaaacata gcggcagga atagaacca 
      aactgtttt gtaagcaag agtagcatt gttggttac 
      tatttacga agacttatc gtaaacttt gataaactc 
      actttttcg gaagttacg agtgtttat ttaactttt 
      aaaaggtac tatgaagat tggcaaaaa agtttaaca 
      acacgtgac cagattgca atagaagtt aataaagtt 
      atttgtccc gatgattgg agtgctaac agaaatcag 
      tggtcaagg cgtcaagct gagttattt atagcgact 
      caagccgcc cttttaacc aataatgaa gaaaaagca 
      ttgccacct caagaactt cacaataga atctccaat 
      ttgctccaa tcaaaccga tctttggca gaagctcac 
      tttttgtca tacttaaat tacttaaga gtgaaggaa 
      ttttgcggc gccattgat agtttatat cactgtttc 
      gacagaagc actttaacc gataccaaa atctcatcg 
      gaggataaa agtaaagtt tacagattt gcggcactc 
      aatctagca attttgcac caccatttc ggccacaag 
      gaagaagct ctcgcctca cttagagag gcaataaaa 
      agctcccaa gttgcaaac gacaacgtt tgcctccaa 
      cacgccctc tgttggctc taccgcctg accccagtc 
      aacaaagaa aaattactc gaacactct gtagccaaa 
      agtttcgaa ctcaaccta tcctacacc gcatcactc 
      ggtattcaa actttcgtc caatacgcg tgtcttatc 
      tcaggccat ccgcaccaa attttcgaa acgttaaca 
      aaaggcgat ttgattaat tgccaacac attttgcgc 
      gatcttatt tcgaacagt tatgcggtc aaatcagcc 
      ctttggcag ttttacggc aagacggaa atgtcgtca 
      ctttggagc cagttattg ttttattta aatattgat 
      aatggacgt agtaatgac gggaaggcg gtctatggt 
      gaaggtttc tgtttggcg atttgtaac gtggccaat 
      caccttcta atgcaagga aattacaat ttggtaaac 
      actgtgttg aattttgcg aaagatcgg tttccaaac 
      gagccaaat tcgcacatt tggatgttg tgcgaaaat 
      ttatttatt ttcgtgcga gcgttacat catgaaaat 
      tggagcgaa gctgaagct gcagctcag aaaattcta 
      gtcgtggat aagtgggag ggttacttg agactagct 
      gagttgtac tactacaag caggattac actgaagcg 
      caccgattt gttgatatt ttgataaat cgttttcac 
      agcgataac aaatacaaa cttaaaatt ttctattac 
      gtgagggcc aaaattttg ctcgcggaa atccaattt 
      tccagttgt tatcccgat aaagtctca ccggaaatt 
      atgactctt cttagcaat tgtctccta gacactgat 
      aaacacaat ttggattat cattccgct ttgattcac 
      ttgcacatt gccaatatt cagttaagt ttgggaatg 
      actagtcag gccctaaaa attttggac cggtgtctt 
      gttcaaatt ctggcacat gggggcaat ttcgataga 
      gctagggcc gttttgttg tacgttaaa tgtctggtg 
      gcgcattct gcgcaactt gaagccact gaaagacgg 
      gagattatt ttaaacgct gcgcaaatg ttggagaaa 
      gtgaaggag gattttagg gctgtggag gcttattcc 
      agggtcaaa gacgtgtta tatttgcag gcgcaactt 
      tacaatagc gttgatttg cggagtgag agaaacaga 
      tgcgctttg gaatttcgg caattggat gaagaaaga 
      acaacgaaa aattattat accctagtt aggtttttg 
      taa
    mRNA sequence
    • >TC001292 RA
      taaaaatgc ccacaaacg tggaaagtc caccttgta 
      aaaaatcca acgttgaag taatgactc cccatagac 
      taaccgttg caattttaa tccgtgatt attgcttat 
      ttcgggatt cagaccggt ttagctgtg aagatgatg 
      gggtgctta aggccagat acagacgag acttttgcg 
      ttttaatat taaagttat tgcaaagtc ctgatctta 
      gtttagaag agttacgga acattttag tctcaacta 
      aatatgaag tgcaccaga gcgttattg aggcttttg 
      atgagacac tagccagtt tatatacaa acggaacag 
      gaactatgc ttgatatta ttgatagtt ttaacagga 
      tcatgactt tcaatgaaa acatagcgg caggaatag 
      aaccaaact gttttgtaa gcaagagta gcattgttg 
      gttactatt tacgaagac ttatcgtaa actttgata 
      aactcactt tttcggaag ttacgagtg tttatttaa 
      cttttaaaa ggtactatg aagattggc aaaaaagtt 
      taacaacac gtgaccaga ttgcaatag aagttaata 
      aagttattt gtcccgatg attggagtg ctaacagaa 
      atcagtggt caaggcgtc aagctgagt tatttatag 
      cgactcaag ccgcccttt taaccaata atgaagaaa 
      aagcattgc cacctcaag aacttcaca atagaatct 
      ccaatttgc tccaatcaa accgatctt tggcagaag 
      ctcactttt tgtcatact taaattact taagagtga 
      aggaatttt gcggcgcca ttgatagtt tatatcact 
      gtttcgaca gaagcactt taaccgata ccaaaatct 
      catcggagg ataaaagta aagtttaca gatttgcgg 
      cactcaatc tagcaattt tgcaccacc atttcggcc 
      acaaggaag aagctctcg cctcactta gagaggcaa 
      taaaaagct cccaagttg caaacgaca acgtttgcc 
      tccaacacg ccctctgtt ggctctacc gcctgaccc 
      cagtcaaca aagaaaaat tactcgaac actctgtag 
      ccaaaagtt tcgaactca acctatcct acaccgcat 
      cactcggta ttcaaactt tcgtccaat acgcgtgtc 
      ttatctcag gccatccgc accaaattt tcgaaacgt 
      taacaaaag gcgatttga ttaattgcc aacacattt 
      tgcgcgatc ttatttcga acagttatg cggtcaaat 
      cagcccttt ggcagtttt acggcaaga cggaaatgt 
      cgtcacttt ggagccagt tattgtttt atttaaata 
      ttgataatg gacgtagta atgacggga aggcggtct 
      atggtgaag gtttctgtt tggcgattt gtaacgtgg 
      ccaatcacc ttctaatgc aaggaaatt acaatttgg 
      taaacactg tgttgaatt ttgcgaaag atcggtttc 
      caaacgagc caaattcgc acatttgga tgttgtgcg 
      aaaatttat ttattttcg tgcgagcgt tacatcatg 
      aaaattgga gcgaagctg aagctgcag ctcagaaaa 
      ttctagtcg tggataagt gggagggtt acttgagac 
      tagctgagt tgtactact acaagcagg attacactg 
      aagcgcacc gatttgttg atattttga taaatcgtt 
      ttcacagcg ataacaaat acaaactta aaattttct 
      attacgtga gggccaaaa ttttgctcg cggaaatcc 
      aattttcca gttgttatc ccgataaag tctcaccgg 
      aaattatga ctcttctta gcaattgtc tcctagaca 
      ctgataaac acaatttgg attatcatt ccgctttga 
      ttcacttgc acattgcca atattcagt taagtttgg 
      gaatgacta gtcaggccc taaaaattt tggaccggt 
      gtcttgttc aaattctgg cacatgggg gcaatttcg 
      atagagcta gggccgttt tgttgtacg ttaaatgtc 
      tggtggcgc attctgcgc aacttgaag ccactgaaa 
      gacgggaga ttattttaa acgctgcgc aaatgttgg 
      agaaagtga aggaggatt ttagggctg tggaggctt 
      attccaggg tcaaagacg tgttatatt tgcaggcgc 
      aactttaca atagcgttg atttgcgga gtgagagaa 
      acagatgcg ctttggaat ttcggcaat tggatgaag 
      aaagaacaa cgaaaaatt attataccc tagttaggt 
      ttttgtaat ttgtgaaat aaacgcatt aattaaga