Status
Please wait ...
Gene information
General information
TC001264 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 7926360..7935218
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC001264
      iBeetle screen iB_00235
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
      Days post injection


      Fertility
      previtellogenic egg chamber not present - Number of animals showing the phenotype: 4

      vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: < 20 remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
      Days post injection


      Fertility
      egg production: number no/almost no eggs -
      egg chamber fused - Number of animals showing the phenotype: 4

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC001264 RA
        MEMCTAQGH VVGMLDRDQ RTAKVCRFD RHVVLVKEY 
        EDSRDQTAL TRRNKTISH RSVPGRTSP HSSCKTRKN 
        RHFWKSLHR LKHALIVGA YLSLFTFPS IESAPTIRT 
        PKSMSRAAS KPKWFNPCG MSAVNGAAP EHRMPDVQL 
        IDQILNQAQ IAEVRAKMF KEEYVHKTF NMDAQQLHN 
        YYKDKDYDW LPTKEIPKN LDQAVPQEH LDTLEFKPT 
        LTTVYSYLQ KFAVGIEQI VWDEEDRNG PFKSSFVQT 
        EDYLRTLLC EIYTAIIEK ELTVEQNVD RDIMGPEFR 
        NMDNTSMNI RNWIIYRDY LNMLEYIIQ VFRHFKAKI 
        E
      CDS sequence
      • >TC001264 RA
        atggagatg tgcacggcc caaggccac gttgttggg 
        atgttagat cgcgatcaa aggaccgcc aaagtgtgc 
        agatttgac aggcacgtg gtgttagtg aaagagtac 
        gaagattcg cgggatcag accgcttta acgagacga 
        aataaaacg attagtcac cgttccgtt ccgggaagg 
        acgagtcca cactcttcg tgcaaaacc aggaaaaat 
        agacacttc tggaagagt ttacacaga ttaaaacac 
        gccctaatc gtcggagcg tacctaagc ctcttcacg 
        tttcccagt attgaatcg gcgccgacg atacgaacc 
        cccaagtcc atgtcgcgg gccgcctcc aagcccaag 
        tggttcaac ccgtgcggc atgagcgcc gtcaacggc 
        gccgccccc gagcaccgc atgcccgac gtccagctc 
        atcgaccag atcctcaac caggcccag atcgccgaa 
        gtcagagcc aagatgttc aaggaggag tatgtccac 
        aaaacattc aacatggac gcgcaacag ctgcacaat 
        tactacaaa gacaaggat tacgactgg ctcccgacc 
        aaggagatc cccaaaaat ttggaccag gcggtcccc 
        caagaacac ctagacacg ttggagttt aaaccgact 
        ctgacaacc gtctacagc tatctccag aaattcgcc 
        gtggggata gaacagatc gtctgggac gaggaggac 
        cggaacgga ccgttcaag tcctcattt gttcaaacc 
        gaagactat cttcgtact ctactctgc gagatttac 
        acagccatc atcgagaag gagctcacg gtggagcaa 
        aacgtcgat cgggacatc atggggccg gagttccgg 
        aacatggac aacacctcc atgaacatc cggaactgg 
        atcatctac agagattat ctcaacatg ctcgaatat 
        atcattcaa gtattccgc cacttcaag gcaaagatc 
        gagtga
      mRNA sequence
      • >TC001264 RA
        tgagttatc cttttgcat ttttgttgc aggaatccg 
        cttagtcgc cccatagcc gttcccgga aggtaagtg 
        tttgaggct tttatcttc aatttccat cgaaacgaa 
        ttcaaaacc gccaagact acctgaacg cgcttgaaa 
        caatacatc aaaattatt ggcgcatat ttcattcgc 
        aagaacaat cagatagtt tcccgggga tgtcataat 
        tcatcgcgt cggtactag aaataactt tcgttggtc 
        gatgcaggt gtgaataaa atttacgat tttccacaa 
        tccggaatt acagttttt cgcgctcga tcgctggtt 
        tcgaaaaaa ccctttgtg tgtgggtta taatttata 
        aatcaagca ttttgtgct cagctggta gtcgtaatg 
        gccatgcaa atatatgga atatattgc aaggtgcaa 
        acttgcctt tctctttca ttgggataa tttatctaa 
        cgtgtctta attcatgaa tgaaaattt ctgctatca 
        atgagcata cacgaataa gaaggcgta gcaaatgtt 
        actcgccca cttctgcat ttccggcca ccgctgtct 
        gacgcacca aagttcttt caacaaccg caaaatcgc 
        tgcttatcg atcatctct tcattttgc tggaagagt 
        cttcgggga aaaatgacg tatcaggct tcaggaacc 
        aaatctatc ggcgcttga attccttca agtctagct 
        cccaattca caatgggcc attatgtgc ggaatttgg 
        tcaaatggc tttgtttgg gccacctag cggcgcgag 
        gaaatttat gtggaggcg ggcttgccg ccaatttgc 
        atttatcga ttattaaat tgaatggaa ttcgaacga 
        gtcgttaga tgatgagga agtgccgta cctgactgg 
        aattggcaa aaccgattt ttcggagaa tcagttgcg 
        cccagcgcc gggaaaatt tcaatgtcc aattttgct 
        gcaaacggt gaaaaccca tcactttgc aaagtcttt 
        gatgtggtg tgcggtttg gtgagtcag tccctggtt 
        tgtaatttg gcgctcgtc ccgtggcgg tgagtgttt 
        atcgcgcca ggatctgtg aattcgggg ccagcggat 
        tcgaatccg ggggagacg gcggcggtt ttctttgat 
        tcgacatgt cttcacggc ggcccatag gccgtttcc 
        ctcatgcac ctgacgaat atctatcgc aagcatttc 
        cgcagtgat tatgctgca ctcacgtat gagaacttc 
        tcgtggaat gtgaacatg ttaataagt gactagtcc 
        caaattctt atcttttga atctggtgt aaacattat 
        ttcccaagg ttttaacaa cttctctgc ctaccagtc 
        gttatttat gggggtcac tagaccggc gacgacttg 
        ctatattat aatattaaa attcaaaat gaaatttat 
        tttgtaata aacgaaatg cggagggtt gacgtgaaa 
        gcaccgact cttaacaac gcacataaa aacaactac 
        gtgttaaaa tttcggata tttctctga cgaggattt 
        ccccaaaaa catgtccta actggtctt aatcccagt 
        gtcaaattt cactttggc tcccgaagg ccgtattta 
        atttacgac tcaaatatt tacaacccc taaatgcta 
        taaacgagg tttttattt tcatctctg taaaattaa 
        tctctgatg agcaattac tattatttc tcgtttacg 
        tttttatca cttgacggc agatctatg caaataatt 
        atcttgtta ttgtatatc ctcttggag ttgtgtaaa 
        attttgtaa tttgatgtt gtctgaggt gtttctcgg 
        aaatcttct aacttttct tcttattct cctcgagca 
        agaaagttt ttagattct tgatttgtc atcctatta 
        tttcgtaaa ttttcgtaa gaatgtcgt tgaccttat 
        tttaaacta ttttcgtca atcgtagaa ttcacaagg 
        tatggaatt tgaaccccg aaagtgatt cacaaatta 
        acaaacgaa aatgctgac tagtagtca tatgcgtgt 
        ttattaata tcataaatt agtcttctg tcgcaagtt 
        aatacagtg gttattaat taattgggg ttttgttta 
        cactttgaa aaaagcgtc cggatcaca aaaattgga 
        aaacaggtc tccccatta atggacctt tccgtttca 
        aagtcgcaa agatcgata tttttactg tacctacct 
        acctattca ctcgtgcct tccccacat aatctaaat 
        tcaagacga tctgttttt aatcgactg ctccataaa 
        aattttaac gatcgtcac ttgcaccat tgtacgtca 
        gacaaggat gtggattac ataagtaga cgaatgaag 
        agatttttt cgaatgtga ttaagcagc ggccgactt 
        tttaaagcc agattgggt tacaaaatg acgtttgtc 
        tcgcctcga ttaacgcag tttcttccg aatgctgag 
        cattgcgaa atttcccaa atcgtgggg gtggttgta 
        aagtttcga tttccgata gctatctgg tgataagag 
        ccaacccat tctgataag attgtgagg tacgcctgg 
        cttgtttag tgtaatcgt acgattgcg tgatacaag 
        aagtcttgt gtttgtgta aaattgggc gggatgttg 
        gggttgcaa acgcgcccc cgattgcgc cacagcgtg 
        tgtgattaa taaagccga ggttaattc tcgtgtgta 
        cctacttat ttcttataa catcgagag acatcaaac 
        acccggaaa tcgtagccg atactggtg gggagatct 
        tatgaatgg tgtgaggtg gagtttaac tgggcacac 
        cctccataa atagttcgt tagtttacg ctttgtcca 
        ctagcagaa tgttgaatg tctgacaga cgtaccgac 
        actgtcggt cggagtccg cggctgtcg acagatttg 
        taaacacac gtgaagttt atccggagt cttctagtc 
        accagtgat cgcagccag tggagagtt ttcggtgat 
        ttcaacgcc ggccgtatt ttctcaaaa tttttcgaa 
        ttcgcaatt tttgattcg tgaacgatg gagatgtgc 
        acggcccaa ggccacgtt gttgggatg ttagatcgc 
        gatcaaagg accgccaaa gtgtgcaga tttgacagg 
        cacgtggtg ttagtgaaa gagtacgaa gattcgcgg 
        gatcagacc gctttaacg agacgaaat aaaacgatt 
        agtcaccgt tccgttccg ggaaggacg agtccacac 
        tcttcgtgc aaaaccagg aaaaataga cacttctgg 
        aagagttta cacagatta aaacacgcc ctaatcgtc 
        ggagcgtac ctaagcctc ttcacgttt cccagtatt 
        gaatcggcg ccgacgata cgaaccccc aagtccatg 
        tcgcgggcc gcctccaag cccaagtgg ttcaacccg 
        tgcggcatg agcgccgtc aacggcgcc gcccccgag 
        caccgcatg cccgacgtc cagctcatc gaccagatc 
        ctcaaccag gcccagatc gccgaagtc agagccaag 
        atgttcaag gaggagtat gtccacaaa acattcaac 
        atggacgcg caacagctg cacaattac tacaaagac 
        aaggattac gactggctc ccgaccaag gagatcccc 
        aaaaatttg gaccaggcg gtcccccaa gaacaccta 
        gacacgttg gagtttaaa ccgactctg acaaccgtc 
        tacagctat ctccagaaa ttcgccgtg gggatagaa 
        cagatcgtc tgggacgag gaggaccgg aacggaccg 
        ttcaagtcc tcatttgtt caaaccgaa gactatctt 
        cgtactcta ctctgcgag atttacaca gccatcatc 
        gagaaggag ctcacggtg gagcaaaac gtcgatcgg 
        gacatcatg gggccggag ttccggaac atggacaac 
        acctccatg aacatccgg aactggatc atctacaga 
        gattatctc aacatgctc gaatatatc attcaagta 
        ttccgccac ttcaaggca aagatcgag tgaagagtt 
        tttaaagta tttaattta tttttctaa tttaattta 
        tataacacg tgtacattt agaagagaa tatatatct 
        atattatat tatttaata gtaaaacaa atgctgtag 
        atattagga actgtgata ttagactta attttttta 
        acgctgttt aattttctc tttccctct ctaatgatg 
        aaaacaaat attatcttc ctgcggttt ggtgacact 
        tttgtgtcg ctgccgcga ctccaccaa atcaaaaca 
        aacacttaa ctgttgtta cggtcacaa cctgacctg 
        aacagtcct ttattctcc cgatattga tttaagcag 
        cggccttga ccgtaacct cattagcgg aatgtaaat 
        ttttcggga aaacactgg cgcggtatt gaccttaaa 
        atgcgtcat gtaaacacg agcgtgctc caaattcta 
        acacgacag aaatcgtaa aatatctaa caagcggcg 
        acttccaaa agatggcgc atggccggg aaaaccaaa 
        cggtaataa tttcccaca cctgaatta tgggtgctt 
        ccctttccc aaatgacgg gacaaattt tcaaagttg 
        cccactcat gcggtgata agatatgaa cgcgaatca 
        aacaataga aaattatga ctttattat ttgcttctg 
        tgattcccc gcaagttgt caaaatgtt tggtgccgg 
        cgtgtgcgt caatgtgcg gcttcgatt gtttggcaa 
        gcctttgaa ggcctcttt tttggtcaa aatatgttt 
        tgcgtatta atgggtgcc gccagacgt cagaagcgt 
        cgcaggaat acaatatgt ggtccaaaa tcgtgaaat 
        aaatcacgt tggccaga