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Gene information
General information
TC001264 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13432903..13441761
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC001264
      iBeetle screen iB_00235

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
      Days post injection

      Fertility
      previtellogenic egg chamber not present - Number of animals showing the phenotype: 4

      vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 4
      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: < 20 remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
      Days post injection

      Fertility
      egg production: number no/almost no eggs -
      egg chamber fused - Number of animals showing the phenotype: 4

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC001264 RA
        MEMCTAQGH VVGMLDRDQ RTAKVCRFD RHVVLVKEY 
EDSRDQTAL TRRNKTISH RSVPGRTSP HSSCKTRKN 
RHFWKSLHR LKHALIVGA YLSLFTFPS IESAPTIRT 
PKSMSRAAS KPKWFNPCG MSAVNGAAP EHRMPDVQL 
IDQILNQAQ IAEVRAKMF KEEYVHKTF NMDAQQLHN 
YYKDKDYDW LPTKEIPKN LDQAVPQEH LDTLEFKPT 
LTTVYSYLQ KFAVGIEQI VWDEEDRNG PFKSSFVQT 
EDYLRTLLC EIYTAIIEK ELTVEQNVD RDIMGPEFR 
NMDNTSMNI RNWIIYRDY LNMLEYIIQ VFRHFKAKI 
E
      CDS sequence
      • >TC001264 RA
        atggagatg tgcacggcc caaggccac gttgttggg 
atgttagat cgcgatcaa aggaccgcc aaagtgtgc 
agatttgac aggcacgtg gtgttagtg aaagagtac 
gaagattcg cgggatcag accgcttta acgagacga 
aataaaacg attagtcac cgttccgtt ccgggaagg 
acgagtcca cactcttcg tgcaaaacc aggaaaaat 
agacacttc tggaagagt ttacacaga ttaaaacac 
gccctaatc gtcggagcg tacctaagc ctcttcacg 
tttcccagt attgaatcg gcgccgacg atacgaacc 
cccaagtcc atgtcgcgg gccgcctcc aagcccaag 
tggttcaac ccgtgcggc atgagcgcc gtcaacggc 
gccgccccc gagcaccgc atgcccgac gtccagctc 
atcgaccag atcctcaac caggcccag atcgccgaa 
gtcagagcc aagatgttc aaggaggag tatgtccac 
aaaacattc aacatggac gcgcaacag ctgcacaat 
tactacaaa gacaaggat tacgactgg ctcccgacc 
aaggagatc cccaaaaat ttggaccag gcggtcccc 
caagaacac ctagacacg ttggagttt aaaccgact 
ctgacaacc gtctacagc tatctccag aaattcgcc 
gtggggata gaacagatc gtctgggac gaggaggac 
cggaacgga ccgttcaag tcctcattt gttcaaacc 
gaagactat cttcgtact ctactctgc gagatttac 
acagccatc atcgagaag gagctcacg gtggagcaa 
aacgtcgat cgggacatc atggggccg gagttccgg 
aacatggac aacacctcc atgaacatc cggaactgg 
atcatctac agagattat ctcaacatg ctcgaatat 
atcattcaa gtattccgc cacttcaag gcaaagatc 
gagtga
      mRNA sequence
      • >TC001264 RA
        tgagttatc cttttgcat ttttgttgc aggaatccg 
cttagtcgc cccatagcc gttcccgga aggtaagtg 
tttgaggct tttatcttc aatttccat cgaaacgaa 
ttcaaaacc gccaagact acctgaacg cgcttgaaa 
caatacatc aaaattatt ggcgcatat ttcattcgc 
aagaacaat cagatagtt tcccgggga tgtcataat 
tcatcgcgt cggtactag aaataactt tcgttggtc 
gatgcaggt gtgaataaa atttacgat tttccacaa 
tccggaatt acagttttt cgcgctcga tcgctggtt 
tcgaaaaaa ccctttgtg tgtgggtta taatttata 
aatcaagca ttttgtgct cagctggta gtcgtaatg 
gccatgcaa atatatgga atatattgc aaggtgcaa 
acttgcctt tctctttca ttgggataa tttatctaa 
cgtgtctta attcatgaa tgaaaattt ctgctatca 
atgagcata cacgaataa gaaggcgta gcaaatgtt 
actcgccca cttctgcat ttccggcca ccgctgtct 
gacgcacca aagttcttt caacaaccg caaaatcgc 
tgcttatcg atcatctct tcattttgc tggaagagt 
cttcgggga aaaatgacg tatcaggct tcaggaacc 
aaatctatc ggcgcttga attccttca agtctagct 
cccaattca caatgggcc attatgtgc ggaatttgg 
tcaaatggc tttgtttgg gccacctag cggcgcgag 
gaaatttat gtggaggcg ggcttgccg ccaatttgc 
atttatcga ttattaaat tgaatggaa ttcgaacga 
gtcgttaga tgatgagga agtgccgta cctgactgg 
aattggcaa aaccgattt ttcggagaa tcagttgcg 
cccagcgcc gggaaaatt tcaatgtcc aattttgct 
gcaaacggt gaaaaccca tcactttgc aaagtcttt 
gatgtggtg tgcggtttg gtgagtcag tccctggtt 
tgtaatttg gcgctcgtc ccgtggcgg tgagtgttt 
atcgcgcca ggatctgtg aattcgggg ccagcggat 
tcgaatccg ggggagacg gcggcggtt ttctttgat 
tcgacatgt cttcacggc ggcccatag gccgtttcc 
ctcatgcac ctgacgaat atctatcgc aagcatttc 
cgcagtgat tatgctgca ctcacgtat gagaacttc 
tcgtggaat gtgaacatg ttaataagt gactagtcc 
caaattctt atcttttga atctggtgt aaacattat 
ttcccaagg ttttaacaa cttctctgc ctaccagtc 
gttatttat gggggtcac tagaccggc gacgacttg 
ctatattat aatattaaa attcaaaat gaaatttat 
tttgtaata aacgaaatg cggagggtt gacgtgaaa 
gcaccgact cttaacaac gcacataaa aacaactac 
gtgttaaaa tttcggata tttctctga cgaggattt 
ccccaaaaa catgtccta actggtctt aatcccagt 
gtcaaattt cactttggc tcccgaagg ccgtattta 
atttacgac tcaaatatt tacaacccc taaatgcta 
taaacgagg tttttattt tcatctctg taaaattaa 
tctctgatg agcaattac tattatttc tcgtttacg 
tttttatca cttgacggc agatctatg caaataatt 
atcttgtta ttgtatatc ctcttggag ttgtgtaaa 
attttgtaa tttgatgtt gtctgaggt gtttctcgg 
aaatcttct aacttttct tcttattct cctcgagca 
agaaagttt ttagattct tgatttgtc atcctatta 
tttcgtaaa ttttcgtaa gaatgtcgt tgaccttat 
tttaaacta ttttcgtca atcgtagaa ttcacaagg 
tatggaatt tgaaccccg aaagtgatt cacaaatta 
acaaacgaa aatgctgac tagtagtca tatgcgtgt 
ttattaata tcataaatt agtcttctg tcgcaagtt 
aatacagtg gttattaat taattgggg ttttgttta 
cactttgaa aaaagcgtc cggatcaca aaaattgga 
aaacaggtc tccccatta atggacctt tccgtttca 
aagtcgcaa agatcgata tttttactg tacctacct 
acctattca ctcgtgcct tccccacat aatctaaat 
tcaagacga tctgttttt aatcgactg ctccataaa 
aattttaac gatcgtcac ttgcaccat tgtacgtca 
gacaaggat gtggattac ataagtaga cgaatgaag 
agatttttt cgaatgtga ttaagcagc ggccgactt 
tttaaagcc agattgggt tacaaaatg acgtttgtc 
tcgcctcga ttaacgcag tttcttccg aatgctgag 
cattgcgaa atttcccaa atcgtgggg gtggttgta 
aagtttcga tttccgata gctatctgg tgataagag 
ccaacccat tctgataag attgtgagg tacgcctgg 
cttgtttag tgtaatcgt acgattgcg tgatacaag 
aagtcttgt gtttgtgta aaattgggc gggatgttg 
gggttgcaa acgcgcccc cgattgcgc cacagcgtg 
tgtgattaa taaagccga ggttaattc tcgtgtgta 
cctacttat ttcttataa catcgagag acatcaaac 
acccggaaa tcgtagccg atactggtg gggagatct 
tatgaatgg tgtgaggtg gagtttaac tgggcacac 
cctccataa atagttcgt tagtttacg ctttgtcca 
ctagcagaa tgttgaatg tctgacaga cgtaccgac 
actgtcggt cggagtccg cggctgtcg acagatttg 
taaacacac gtgaagttt atccggagt cttctagtc 
accagtgat cgcagccag tggagagtt ttcggtgat 
ttcaacgcc ggccgtatt ttctcaaaa tttttcgaa 
ttcgcaatt tttgattcg tgaacgatg gagatgtgc 
acggcccaa ggccacgtt gttgggatg ttagatcgc 
gatcaaagg accgccaaa gtgtgcaga tttgacagg 
cacgtggtg ttagtgaaa gagtacgaa gattcgcgg 
gatcagacc gctttaacg agacgaaat aaaacgatt 
agtcaccgt tccgttccg ggaaggacg agtccacac 
tcttcgtgc aaaaccagg aaaaataga cacttctgg 
aagagttta cacagatta aaacacgcc ctaatcgtc 
ggagcgtac ctaagcctc ttcacgttt cccagtatt 
gaatcggcg ccgacgata cgaaccccc aagtccatg 
tcgcgggcc gcctccaag cccaagtgg ttcaacccg 
tgcggcatg agcgccgtc aacggcgcc gcccccgag 
caccgcatg cccgacgtc cagctcatc gaccagatc 
ctcaaccag gcccagatc gccgaagtc agagccaag 
atgttcaag gaggagtat gtccacaaa acattcaac 
atggacgcg caacagctg cacaattac tacaaagac 
aaggattac gactggctc ccgaccaag gagatcccc 
aaaaatttg gaccaggcg gtcccccaa gaacaccta 
gacacgttg gagtttaaa ccgactctg acaaccgtc 
tacagctat ctccagaaa ttcgccgtg gggatagaa 
cagatcgtc tgggacgag gaggaccgg aacggaccg 
ttcaagtcc tcatttgtt caaaccgaa gactatctt 
cgtactcta ctctgcgag atttacaca gccatcatc 
gagaaggag ctcacggtg gagcaaaac gtcgatcgg 
gacatcatg gggccggag ttccggaac atggacaac 
acctccatg aacatccgg aactggatc atctacaga 
gattatctc aacatgctc gaatatatc attcaagta 
ttccgccac ttcaaggca aagatcgag tgaagagtt 
tttaaagta tttaattta tttttctaa tttaattta 
tataacacg tgtacattt agaagagaa tatatatct 
atattatat tatttaata gtaaaacaa atgctgtag 
atattagga actgtgata ttagactta attttttta 
acgctgttt aattttctc tttccctct ctaatgatg 
aaaacaaat attatcttc ctgcggttt ggtgacact 
tttgtgtcg ctgccgcga ctccaccaa atcaaaaca 
aacacttaa ctgttgtta cggtcacaa cctgacctg 
aacagtcct ttattctcc cgatattga tttaagcag 
cggccttga ccgtaacct cattagcgg aatgtaaat 
ttttcggga aaacactgg cgcggtatt gaccttaaa 
atgcgtcat gtaaacacg agcgtgctc caaattcta 
acacgacag aaatcgtaa aatatctaa caagcggcg 
acttccaaa agatggcgc atggccggg aaaaccaaa 
cggtaataa tttcccaca cctgaatta tgggtgctt 
ccctttccc aaatgacgg gacaaattt tcaaagttg 
cccactcat gcggtgata agatatgaa cgcgaatca 
aacaataga aaattatga ctttattat ttgcttctg 
tgattcccc gcaagttgt caaaatgtt tggtgccgg 
cgtgtgcgt caatgtgcg gcttcgatt gtttggcaa 
gcctttgaa ggcctcttt tttggtcaa aatatgttt 
tgcgtatta atgggtgcc gccagacgt cagaagcgt 
cgcaggaat acaatatgt ggtccaaaa tcgtgaaat 
aaatcacgt tggccaga
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY