Status
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Gene information
General information
TC001262 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13334305..13337641
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001262
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00234

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Pupal morphology
    oblique dorsal thoracic muscles anterior shortened - Number of animals showing the phenotype: 4

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001262 RA
      MWLQVFGFG LLLASSGAL PTQVHDVSR AKIQKYGSE 
      NRLPTNVEP KNYALNLNL AEDFATSKV FSGSVELKI 
      VVTSSANIK SFKLHAKNL TIDTKSIKL SENDADNIF 
      DKLEGPDTE TDFVTITAK SDLVSGTTY TLKIEYTGT 
      LSDTEMAGF YLSTYKDKD SDEVKYLAT TQFEDTGAR 
      RVFPCFDEP ALKAEFDIS ITYPSKYTA LSNTPNVST 
      TTLDPNAKL KTTKFNTTP TMSTYLVAF VISEFQCTD 
      VKKEDDFTT QVCSRPGTQ DLAALANAV PAKIVQVMM 
      DYTKVNAIK TGQLVQVAV PDMAPGAME NWGLLIYRE 
      AYLLSDDND SSVYEKQHT VTVVAHEIS HQWFGDLIT 
      LDWWSDTFL NEGFATYFE YHAPDVEHT DWELQKQFV 
      IEQLQTALV TDSKNTSLP LSWDVDDPD FMASRFDDI 
      SYNKGGSIL RMVHHFQKL DNFKQGLTT YIANAGQVG 
      TPDILWNSF NFSEGLNVS MPKIMHSWT KEPGYPLLT 
      VTTSDKSVK ISQEAFTLS GDAPKTKWY VPITYAGSN 
      SKEFTDTET KAWLLPDEE LTLDSVLEK NDWIVLNNQ 
      QVGYYRVDY DDNLWSKIM ASLEKDVES IDEISRAQI 
      VDDLLNMAR AGKKDYGKV LGQIKFLQN DTSYYSWYS 
      ALANFDYLL PKVDDSVSK ALNEYLSDL IKTFVESVP 
      IDKMNTTNH INTLKQVLA LKWACRLNL SDCVTKTQK 
      MFDDYKGNE TNKPDPNLR SVVYCTALS NSKNASEDF 
      NFLDKQYDS AELASEKVT ILKSLGCVN DANTRKDYL 
      KKALTDKVR RQDAKYVFS SVYSIGNEG VDDILDFIK 
      ENQTDFKNR FGSTSTLSS LISELADFI NTVDQVQKL 
      TDFVDSAAE PEYKTAAQD AVKIGQDNI EQNNKLKEQ 
      LKKYFSDDD DNNAGAAEF ANVALVLTG YLLFNFV
    CDS sequence
    • >TC001262 RA
      atgtggtta caagtgttc ggtttcgga ttattgctt 
      gcgagcagt ggagctttg cccacccaa gtccacgat 
      gtttctaga gccaaaata caaaaatat ggcagcgaa 
      aatcgcctt ccgacaaac gtggaaccc aaaaattat 
      gctttaaat ttaaatctt gccgaagat ttcgccaca 
      agtaaagtc ttctctggc agtgttgaa ctcaaaata 
      gtggtcacc agtagtgct aatataaag tcgttcaaa 
      ctccatgcg aaaaacctg actattgat actaaatca 
      ataaagcta tctgaaaat gatgccgat aatattttt 
      gacaagctg gagggacca gacacagaa acagacttt 
      gtgacaatc actgccaag agtgacttg gttagtgga 
      accacttat accttgaaa attgaatat actggaaca 
      ctgagcgat actgaaatg gctgggttt tacctaagt 
      acctacaag gacaaggat tctgatgag gttaaatat 
      ttagccaca acacagttt gaagacaca ggggctcgg 
      agggttttc ccttgcttt gacgaacct gccttgaaa 
      gcggaattt gatataagt attacgtac ccaagcaaa 
      tacactgca ctatcaaat acgccaaat gtttcaaca 
      actacttta gatccaaac gcgaagtta aaaactacc 
      aagtttaac acaactcca accatgtca acttatttg 
      gttgcgttc gtcatatca gaatttcag tgcacggac 
      gtgaaaaaa gaagacgat tttacgacc caagtgtgt 
      tccagacct ggaacccaa gatttagca gctcttgct 
      aacgcagta ccagctaaa atcgtccaa gtcatgatg 
      gactatact aaagttaat gctattaag acgggtcag 
      ttggtacaa gtggcggtc ccagatatg gccccagga 
      gccatggaa aattgggga ttgttgatt tacagggaa 
      gcgtattta ctttctgat gataatgac tcgtctgtt 
      tacgaaaaa caacacaca gtcacggtg gtagctcat 
      gaaatatct catcaatgg tttggagat ctcatcact 
      ctagattgg tggagcgac actttccta aacgaaggt 
      ttcgccaca tatttcgaa tatcatgct cctgacgtt 
      gaacatact gactgggaa ttgcaaaaa caattcgta 
      attgaacaa ctccaaaca gctctggtc acagattcg 
      aaaaacacg tccttgccg ctttcgtgg gatgtagac 
      gatcctgat ttcatggcc agcagattt gatgatatt 
      tcgtacaac aaaggtggt agcattttg cgaatggtt 
      caccacttc caaaaattg gataacttc aagcaagga 
      ctcaccact tatattgcg aacgcgggt caagttggc 
      acaccagac attctctgg aactccttt aatttttcc 
      gagggacta aacgtatca atgcccaaa ataatgcac 
      agttggacc aaagagcca ggatatccg ctcctcaca 
      gtcacaact tcagacaaa agtgttaaa ataagtcag 
      gaagcattt actctatct ggagatgcc cccaaaact 
      aaatggtac gtccccata acttacgct ggatcaaac 
      tcaaaagaa ttcactgac acggaaacg aaagcctgg 
      cttttgccg gacgaggaa ctaactttg gactcagtt 
      ctggaaaag aatgactgg attgttcta aacaatcag 
      caagttggg tactaccgc gtcgattac gatgacaat 
      ttgtggtcg aaaataatg gcaagtctt gagaaagat 
      gttgaaagt attgacgaa atcagtcga gctcagatc 
      gtggatgac ttactaaat atggcccga gcgggcaaa 
      aaagactac ggcaaagtg ttggggcaa ataaaattc 
      ctgcaaaat gatacctcg tactacagc tggtactcg 
      gctttggcc aattttgac tatcttttg cccaaagtt 
      gacgatagt gtttcgaag gcgcttaat gaatatttg 
      tccgatttg atcaagact tttgttgaa tcggttcca 
      atagataag atgaacact accaaccac attaacacg 
      cttaagcaa gttttggcc ctgaagtgg gcctgcagg 
      ttgaatttg tccgactgt gtgaccaag acccaaaaa 
      atgtttgat gattacaaa gggaatgaa accaacaaa 
      ccagatcct aatttaaga agtgttgta tattgtacg 
      gccctcagt aattcgaaa aatgcatcg gaagatttt 
      aatttcctt gacaagcag tacgacagt gctgaactg 
      gcaagcgag aaagtgaca attttgaaa agcttgggt 
      tgcgtcaat gatgctaac acgcgaaaa gattacttg 
      aaaaaagcc ctaacggat aaggtcaga cgacaggat 
      gcgaaatat gttttttct tcggtttat tcaatcggg 
      aacgagggt gttgatgac attttggac tttattaaa 
      gaaaatcaa accgatttt aaaaatcgt ttcggttct 
      acgtctacc ctgagttct ctaatcagt gaactcgct 
      gattttatt aatactgta gatcaagtc caaaaattg 
      acggatttc gtcgatagt gcggctgag cctgaatac 
      aaaactgca gcacaagat gctgttaaa attggacaa 
      gacaacata gaacagaat aacaagcta aaagaacaa 
      ttgaagaaa tatttcagt gatgatgat gataacaat 
      gccggcgcg gctgagttt gcaaatgtt gcattggtt 
      ctaacggga tatctcctg tttaatttt gtctaa
    mRNA sequence
    • >TC001262 RA
      atgtggtta caagtgttc ggtttcgga ttattgctt 
      gcgagcagt ggagctttg cccacccaa gtccacgat 
      gtttctaga gccaaaata caaaaatat ggcagcgaa 
      aatcgcctt ccgacaaac gtggaaccc aaaaattat 
      gctttaaat ttaaatctt gccgaagat ttcgccaca 
      agtaaagtc ttctctggc agtgttgaa ctcaaaata 
      gtggtcacc agtagtgct aatataaag tcgttcaaa 
      ctccatgcg aaaaacctg actattgat actaaatca 
      ataaagcta tctgaaaat gatgccgat aatattttt 
      gacaagctg gagggacca gacacagaa acagacttt 
      gtgacaatc actgccaag agtgacttg gttagtgga 
      accacttat accttgaaa attgaatat actggaaca 
      ctgagcgat actgaaatg gctgggttt tacctaagt 
      acctacaag gacaaggat tctgatgag gttaaatat 
      ttagccaca acacagttt gaagacaca ggggctcgg 
      agggttttc ccttgcttt gacgaacct gccttgaaa 
      gcggaattt gatataagt attacgtac ccaagcaaa 
      tacactgca ctatcaaat acgccaaat gtttcaaca 
      actacttta gatccaaac gcgaagtta aaaactacc 
      aagtttaac acaactcca accatgtca acttatttg 
      gttgcgttc gtcatatca gaatttcag tgcacggac 
      gtgaaaaaa gaagacgat tttacgacc caagtgtgt 
      tccagacct ggaacccaa gatttagca gctcttgct 
      aacgcagta ccagctaaa atcgtccaa gtcatgatg 
      gactatact aaagttaat gctattaag acgggtcag 
      ttggtacaa gtggcggtc ccagatatg gccccagga 
      gccatggaa aattgggga ttgttgatt tacagggaa 
      gcgtattta ctttctgat gataatgac tcgtctgtt 
      tacgaaaaa caacacaca gtcacggtg gtagctcat 
      gaaatatct catcaatgg tttggagat ctcatcact 
      ctagattgg tggagcgac actttccta aacgaaggt 
      ttcgccaca tatttcgaa tatcatgct cctgacgtt 
      gaacatact gactgggaa ttgcaaaaa caattcgta 
      attgaacaa ctccaaaca gctctggtc acagattcg 
      aaaaacacg tccttgccg ctttcgtgg gatgtagac 
      gatcctgat ttcatggcc agcagattt gatgatatt 
      tcgtacaac aaaggtggt agcattttg cgaatggtt 
      caccacttc caaaaattg gataacttc aagcaagga 
      ctcaccact tatattgcg aacgcgggt caagttggc 
      acaccagac attctctgg aactccttt aatttttcc 
      gagggacta aacgtatca atgcccaaa ataatgcac 
      agttggacc aaagagcca ggatatccg ctcctcaca 
      gtcacaact tcagacaaa agtgttaaa ataagtcag 
      gaagcattt actctatct ggagatgcc cccaaaact 
      aaatggtac gtccccata acttacgct ggatcaaac 
      tcaaaagaa ttcactgac acggaaacg aaagcctgg 
      cttttgccg gacgaggaa ctaactttg gactcagtt 
      ctggaaaag aatgactgg attgttcta aacaatcag 
      caagttggg tactaccgc gtcgattac gatgacaat 
      ttgtggtcg aaaataatg gcaagtctt gagaaagat 
      gttgaaagt attgacgaa atcagtcga gctcagatc 
      gtggatgac ttactaaat atggcccga gcgggcaaa 
      aaagactac ggcaaagtg ttggggcaa ataaaattc 
      ctgcaaaat gatacctcg tactacagc tggtactcg 
      gctttggcc aattttgac tatcttttg cccaaagtt 
      gacgatagt gtttcgaag gcgcttaat gaatatttg 
      tccgatttg atcaagact tttgttgaa tcggttcca 
      atagataag atgaacact accaaccac attaacacg 
      cttaagcaa gttttggcc ctgaagtgg gcctgcagg 
      ttgaatttg tccgactgt gtgaccaag acccaaaaa 
      atgtttgat gattacaaa gggaatgaa accaacaaa 
      ccagatcct aatttaaga agtgttgta tattgtacg 
      gccctcagt aattcgaaa aatgcatcg gaagatttt 
      aatttcctt gacaagcag tacgacagt gctgaactg 
      gcaagcgag aaagtgaca attttgaaa agcttgggt 
      tgcgtcaat gatgctaac acgcgaaaa gattacttg 
      aaaaaagcc ctaacggat aaggtcaga cgacaggat 
      gcgaaatat gttttttct tcggtttat tcaatcggg 
      aacgagggt gttgatgac attttggac tttattaaa 
      gaaaatcaa accgatttt aaaaatcgt ttcggttct 
      acgtctacc ctgagttct ctaatcagt gaactcgct 
      gattttatt aatactgta gatcaagtc caaaaattg 
      acggatttc gtcgatagt gcggctgag cctgaatac 
      aaaactgca gcacaagat gctgttaaa attggacaa 
      gacaacata gaacagaat aacaagcta aaagaacaa 
      ttgaagaaa tatttcagt gatgatgat gataacaat 
      gccggcgcg gctgagttt gcaaatgtt gcattggtt 
      ctaacggga tatctcctg tttaatttt gtctaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY