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Gene information
General information
TC001259 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13318721..13322480
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001259
    iBeetle screen iB_00232

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001259 RA
      MSLFWLFLT LLLAHTKST PIEPKTVEY RLPEGAVKV 
      IHYDIALNL KKDIFDTNT FTGVTYILF KNLKSTNEI 
      RLHANGLDF SEVLLAAAD AKQINFENK DFVSDPASD 
      ILTLTTSTN LEAGAEYQL KFTYQAKLR TDEMYGFYK 
      SSYKAPNGT TVYLATTQF QATHARKAF PCFDEPIYK 
      ALFDIKITH PSIYKAVGN TRGSTQTIN QDYSATSFA 
      TTPAMSTYL IAFIVSDFT CSGSTPIEE AIPHLVCSR 
      NEAVSTREL AEKVGPKLM WAMENFTGI AYNVSKITK 
      VHQVAIPDF AAGAMENWG LVTYRETAL LWDPEESSN 
      RYKQRVETV IAHELSHFW FGDLVTTHW WSDTFLNEG 
      FATYFEYFA TAEIENDWE MEKQFVIEQ LHPVLASDA 
      SQTSNPLSS SVSSQAQIQ GRFNSISYN KGGSIFRMV 
      RHIMGEENF KAGIHAYLE KHKFGSTTP TDLWDALTP 
      FAKFLLPLN KTFSIVMDD WVNKPGYPV LTVRTNYSG 
      LVLTQTRFV ASGEAEKGK WFIPLSYTT ASDTNKFQS 
      TSVKLWYMP LGVLNLLYT PKNKTDWII FNNQQTGYY 
      RVDYDDILW KRIEVALNK PGFDGIHEI NRAQLLDDL 
      HNFARYGIH TYTELFDFY RYLKKETSY YPWYAAMTA 
      FSTMLARTG DERIRTSLS EFILELIEI FYNTHPFNS 
      QKNDNQIYT HSRVLASTW ACRLGLHDC VKNALDAFN 
      NYKTSQKRP DKNLRSVIY CNGLRHSND TTNDWEFLW 
      KDYLSTSIA TEQVTILSA LGCAKDTTV LTNYLEKSI 
      NSTSGIRPQ DALSVFSSV YTGNPEGVD IALDFLLKN 
      YNKIAAHYG SMNAASSLF SGLASRFTR KDQTDKLSN 
      FLDTATDLS ETLRNSARA ALANALINL EWIEKFERE 
      LTLYFRLEE EETTPSTPS TSSPTTPKD EENNSGVAS 
      TSGFATITA TMCIFYFYY
    CDS sequence
    • >TC001259 RA
      atgtcgctc ttttggctt tttctcacc ctgctatta 
      gcacacact aagtctacc ccaattgaa ccgaaaacc 
      gtcgagtat cgcttaccc gaaggtgcc gtcaaagtg 
      atccactat gacattgct ctaaacctc aaaaaagat 
      attttcgat accaatacc ttcactgga gtaacttac 
      attttattt aaaaatctg aaaagtacc aacgaaatt 
      cggttacat gccaatggc ttggacttc tctgaagta 
      ctactagca gcggctgat gccaaacaa atcaatttt 
      gaaaataaa gattttgtt agcgaccct gctagtgat 
      atactcaca cttaccaca agtaccaac ttggaggct 
      ggtgcagag taccagctt aaattcaca tatcaggca 
      aaattacgt accgatgaa atgtacggt ttttacaag 
      agttcctac aaggcacct aatggtact accgtttat 
      ctagcaacg acccagttt caggcaact catgccagg 
      aaggctttc ccttgcttt gatgaaccg atatataaa 
      gcacttttc gatattaaa ataacccat cctagtatc 
      tacaaagct gttggtaat actagaggt agtacccaa 
      actattaat caggattat agtgctact agttttgcc 
      acaaccccg gccatgtcc acatatttg atagctttc 
      atcgtttcg gatttcacg tgttctggt agtactccg 
      attgaggaa gccatccct cacctggtc tgttcacga 
      aatgaagct gtttccact agggaattg gccgaaaaa 
      gttgggcct aagttaatg tgggccatg gaaaacttc 
      acaggaatt gcgtacaac gtgtccaaa attacgaaa 
      gtacaccag gttgcgatt ccagatttt gcagcaggt 
      gctatggaa aactgggga ctagtgact tatcgggag 
      actgcgctt ttgtgggac cctgaagag tcctctaac 
      cgctacaag caaagggtc gaaacggtg atagcacac 
      gaattgtcg catttttgg ttcggtgat ttagttacg 
      acgcattgg tggtcggac actttccta aacgagggt 
      tttgccacc tattttgag tactttgcc acagctgaa 
      attgaaaat gactgggaa atggagaaa caattcgtg 
      attgaacaa ctccaccct gttctagca agcgatgcc 
      tcacaaact tccaaccct ttatcatcc tcagtatcg 
      tcacaagcg cagattcaa ggccggttc aattcaatt 
      tcgtacaat aaaggtggt agcatattc agaatggtg 
      cggcacata atgggcgaa gaaaatttc aaagccggc 
      attcacgcc tatttggag aaacacaag tttggctca 
      actacaccg accgatctc tgggacgct ttaaccccc 
      tttgccaaa tttttactt cccctaaat aaaacattt 
      tccattgtt atggatgat tgggtaaac aagcccgga 
      tatccagtc ctgaccgtc cgaactaat tattcaggc 
      cttgtgctt acacaaacg agatttgtg gcttctggg 
      gaagcggaa aaaggaaaa tggtttatt cctttgagt 
      tacaccacg gcctcagac acgaataag ttccaaagc 
      acgtcggtt aagctttgg tacatgccg ttaggcgtc 
      ttgaatctt ctatacacg ccgaaaaat aagactgat 
      tggatcatt ttcaataat caacagaca ggatattac 
      agagtcgat tacgatgac atcctttgg aaacggatt 
      gaagtggct ttgaacaag cccgggttt gatggcatt 
      cacgaaata aatcgggca caattactc gacgatttg 
      cacaatttt gcaagatat ggcatccac acttacaca 
      gaattattc gatttctac cgatacttg aaaaaagaa 
      acctcgtac tacccgtgg tacgccgcc atgactgct 
      ttttcaacc atgttggcc agaacagga gatgaaaga 
      atacgaaca agtctttcg gagtttatt ttagaactg 
      attgaaatt ttttacaac acgcatcct ttcaacagt 
      caaaaaaat gataatcaa atctataca cacagcagg 
      gttttggcc tcgacttgg gcttgcaga ctggggctc 
      cacgattgt gttaaaaat gctttagat gcctttaat 
      aattacaaa acttcgcaa aagagacct gataaaaat 
      ctgaggtct gtgatttat tgcaacggc ttgagacac 
      agcaatgat actacaaat gactgggaa ttcctctgg 
      aaggactac ctgagtacg agcatagcc acggaacaa 
      gtcacgatt ttgtcggct cttggttgt gccaaagac 
      acaacagta cttaccaat tatttggaa aaatcgatc 
      aatagtact tcgggaatt cggccacaa gacgccctt 
      tccgtgttc tcctcggtg tacaccggc aatcctgaa 
      ggtgtcgac atcgcgctt gattttctc ctcaaaaat 
      tacaacaaa attgcggct cattatggt agcatgaac 
      gcggcgagt tctctattc tcaggttta gcgagccga 
      tttacacga aaggaccaa accgacaag ttgagcaat 
      ttcctagac actgccacc gatttgtcg gaaactttg 
      cgaaattcc gcacgtgcg gctctcgcc aatgcgtta 
      ataaaccta gagtggatc gagaaattt gaacgggaa 
      ttgaccctt tattttaga cttgaagag gaagaaact 
      acgccttct acgccttcc acatcgtca cccacgact 
      ccgaaagat gaggaaaat aattcagga gtggcgtca 
      acgagcgga tttgcaaca ataacagcc acgatgtgt 
      attttctac ttctattat taa
    mRNA sequence
    • >TC001259 RA
      tcaaacaat taacacttc gagtttatg gttctgagg 
      atgacagag tagcccaca acaataaat gtattagca 
      gcttcattc tgtgataag attataaaa tataaaaaa 
      tagaaaact attagtttt accagtttt aaccttctg 
      agagccata atgtcgctc ttttggctt tttctcacc 
      ctgctatta gcacacact aagtctacc ccaattgaa 
      ccgaaaacc gtcgagtat cgcttaccc gaaggtgcc 
      gtcaaagtg atccactat gacattgct ctaaacctc 
      aaaaaagat attttcgat accaatacc ttcactgga 
      gtaacttac attttattt aaaaatctg aaaagtacc 
      aacgaaatt cggttacat gccaatggc ttggacttc 
      tctgaagta ctactagca gcggctgat gccaaacaa 
      atcaatttt gaaaataaa gattttgtt agcgaccct 
      gctagtgat atactcaca cttaccaca agtaccaac 
      ttggaggct ggtgcagag taccagctt aaattcaca 
      tatcaggca aaattacgt accgatgaa atgtacggt 
      ttttacaag agttcctac aaggcacct aatggtact 
      accgtttat ctagcaacg acccagttt caggcaact 
      catgccagg aaggctttc ccttgcttt gatgaaccg 
      atatataaa gcacttttc gatattaaa ataacccat 
      cctagtatc tacaaagct gttggtaat actagaggt 
      agtacccaa actattaat caggattat agtgctact 
      agttttgcc acaaccccg gccatgtcc acatatttg 
      atagctttc atcgtttcg gatttcacg tgttctggt 
      agtactccg attgaggaa gccatccct cacctggtc 
      tgttcacga aatgaagct gtttccact agggaattg 
      gccgaaaaa gttgggcct aagttaatg tgggccatg 
      gaaaacttc acaggaatt gcgtacaac gtgtccaaa 
      attacgaaa gtacaccag gttgcgatt ccagatttt 
      gcagcaggt gctatggaa aactgggga ctagtgact 
      tatcgggag actgcgctt ttgtgggac cctgaagag 
      tcctctaac cgctacaag caaagggtc gaaacggtg 
      atagcacac gaattgtcg catttttgg ttcggtgat 
      ttagttacg acgcattgg tggtcggac actttccta 
      aacgagggt tttgccacc tattttgag tactttgcc 
      acagctgaa attgaaaat gactgggaa atggagaaa 
      caattcgtg attgaacaa ctccaccct gttctagca 
      agcgatgcc tcacaaact tccaaccct ttatcatcc 
      tcagtatcg tcacaagcg cagattcaa ggccggttc 
      aattcaatt tcgtacaat aaaggtggt agcatattc 
      agaatggtg cggcacata atgggcgaa gaaaatttc 
      aaagccggc attcacgcc tatttggag aaacacaag 
      tttggctca actacaccg accgatctc tgggacgct 
      ttaaccccc tttgccaaa tttttactt cccctaaat 
      aaaacattt tccattgtt atggatgat tgggtaaac 
      aagcccgga tatccagtc ctgaccgtc cgaactaat 
      tattcaggc cttgtgctt acacaaacg agatttgtg 
      gcttctggg gaagcggaa aaaggaaaa tggtttatt 
      cctttgagt tacaccacg gcctcagac acgaataag 
      ttccaaagc acgtcggtt aagctttgg tacatgccg 
      ttaggcgtc ttgaatctt ctatacacg ccgaaaaat 
      aagactgat tggatcatt ttcaataat caacagaca 
      ggatattac agagtcgat tacgatgac atcctttgg 
      aaacggatt gaagtggct ttgaacaag cccgggttt 
      gatggcatt cacgaaata aatcgggca caattactc 
      gacgatttg cacaatttt gcaagatat ggcatccac 
      acttacaca gaattattc gatttctac cgatacttg 
      aaaaaagaa acctcgtac tacccgtgg tacgccgcc 
      atgactgct ttttcaacc atgttggcc agaacagga 
      gatgaaaga atacgaaca agtctttcg gagtttatt 
      ttagaactg attgaaatt ttttacaac acgcatcct 
      ttcaacagt caaaaaaat gataatcaa atctataca 
      cacagcagg gttttggcc tcgacttgg gcttgcaga 
      ctggggctc cacgattgt gttaaaaat gctttagat 
      gcctttaat aattacaaa acttcgcaa aagagacct 
      gataaaaat ctgaggtct gtgatttat tgcaacggc 
      ttgagacac agcaatgat actacaaat gactgggaa 
      ttcctctgg aaggactac ctgagtacg agcatagcc 
      acggaacaa gtcacgatt ttgtcggct cttggttgt 
      gccaaagac acaacagta cttaccaat tatttggaa 
      aaatcgatc aatagtact tcgggaatt cggccacaa 
      gacgccctt tccgtgttc tcctcggtg tacaccggc 
      aatcctgaa ggtgtcgac atcgcgctt gattttctc 
      ctcaaaaat tacaacaaa attgcggct cattatggt 
      agcatgaac gcggcgagt tctctattc tcaggttta 
      gcgagccga tttacacga aaggaccaa accgacaag 
      ttgagcaat ttcctagac actgccacc gatttgtcg 
      gaaactttg cgaaattcc gcacgtgcg gctctcgcc 
      aatgcgtta ataaaccta gagtggatc gagaaattt 
      gaacgggaa ttgaccctt tattttaga cttgaagag 
      gaagaaact acgccttct acgccttcc acatcgtca 
      cccacgact ccgaaagat gaggaaaat aattcagga 
      gtggcgtca acgagcgga tttgcaaca ataacagcc 
      acgatgtgt attttctac ttctattat taagtaaat 
      aatttattg ttttgtaat ctattaagt gccataatt 
      agcgataag tcaagccta caatcgaaa ttgcaaaat 
      tgtaaagtt ttatttcgc acaaataaa cttgaccca 
      aaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY