| General information | |||
| TC032214
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 12845529..12951479 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC032214 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00231 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC032214 RA
MERARVSFF KLVIFVTVF VEMKFDVYA DFENSWTMF MEQPCCSGT GNHHVRHHR DHVREFSCG MLYYRTFYV DSKRDSLYV GAMDRVYRL NLSNINQSS CERDALNLE PTNVASCVS KGKSEHFDC RNHIRVIQS MGDGSRLYI CGTNAHNPK DWVVNANLT HLSRNTFVP GIGMGIAKC PYDPADNST AVWVERGNP GDLPGLYSG TNAEFTKAD TVIFRTDLY NLTTGRKEF SFKRTLKYD SKWLDKPNF VGSFDIGDY VLFFFRETA VEYINCGKS VYSRVARVC KKDTGGKNI LSQNWATYL KARLNCSIP GEFPFYFNE IQSIYKVPG DDTKFYGTF TTSTNGLMG SAICSFTLD DIQQAFSGK FKEQASSSS AWLPVLSNR VPEPRPGTC VNDTETLPD TVLNFIRSH PLMDSAVMH EHEKPVFFK RDVFFTRLV VDKIKVDIG GAVLDYTVY YAGTNEGRV HKIVEWQRD TDGTSSILL DVFDVTPGE PIQVMEISK ENKALYVAS DYRVKQVDL VMCNRRYDN CLRCVHDPY CGWDKDSNV CKPYSPGLL QDVSNSTVD VCDSSVVKK KMIVTWGQS LHLGCFLKM PAVLASQTI TWYHYSKEK GRYKIQYRP DKYIETSEH GLVIIAVTE ADAGRYDCW MGASLLCSY NVTVDAHRC SPPAKSNDY QKIYSDWCH EFEKYKSAM KTWEKKQAQ CASRQNDSN QNSHPNEIY RPLV
- >TC032214 RB
MKEGWKSDM ILMSTEDQS LLTRSDRSE ERDACTNHV REFSCGMLY YRTFYVDSK RDSLYVGAM DRVYRLNLS NINQSSCER DALNLEPTN VASCVSKGK SEHFDCRNH IRVIQSMGD GSRLYICGT NAHNPKDWV VNANLTHLS RNTFVPGIG MGIAKCPYD PADNSTAVW VERGNPGDL PGLYSGTNA EFTKADTVI FRTDLYNLT TGRKEFSFK RTLKYDSKW LDKPNFVGS FDIGDYVLF FFRETAVEY INCGKSVYS RVARVCKKD TGGKNILSQ NWATYLKAR LNCSIPGEF PFYFNEIQS IYKVPGDDT KFYGTFTTS TNGLMGSAI CSFTLDDIQ QAFSGKFKE QASSSSAWL PVLSNRVPE PRPGTCVND TETLPDTVL NFIRSHPLM DSAVMHEHE KPVFFKRDV FFTRLVVDK IKVDIGGAV LDYTVYYAG TNEGRVHKI VEWQRDTDG TSSILLDVF DVTPGEPIQ VMEISKENK ALYVASDYR VKQVDLVMC NRRYDNCLR CVHDPYCGW DKDSNVCKP YSPGLLQDV SNSTVDVCD SSVVKKKMI VTWGQSLHL GCFLKMPAV LASQTITWY HYSKEKGRY KIQYRPDKY IETSEHGLV IIAVTEADA GRYDCWMGA SLLCSYNVT VDAHRCSPP AKSNDYQKI YSDWCHEFE KYKSAMKTW EKKQAQCAS RQNDSNQNS HPNEIYRPL V
- >TC032214 RA
atggagcgt gctagagtt tccttcttc aagctggtt atttttgtg actgtgttc gtggaaatg aagttcgat gtttatgct gattttgag aacagttgg accatgttc atggaacaa ccctgttgt tccggcacc ggaaaccac catgtcagg catcacaga gatcatgtg cgagagttc tcttgtggg atgttgtac taccgaacg ttctacgtg gacagcaaa agagattcg ctttacgtt ggagcaatg gatcgtgtc taccgactg aacctcagc aacatcaac caatccagc tgcgagcga gatgccctg aatctggag ccgacgaac gtggcgagc tgcgtttcc aaaggcaaa tcagagcat ttcgactgc cgcaaccac atccgggtt atccaatcg atgggcgat gggagccga ctgtacatt tgtgggacc aacgctcac aaccccaag gactgggtg gttaacgcg aacttgacg catctcagt cggaacacc ttcgtacct ggcattgga atgggcatt gccaaatgc ccctacgat ccagctgac aattcgacg gccgtttgg gtcgaaagg ggaaacccc ggagacttg cccggactt tattccgga accaacgcc gagtttacc aaggcagac actgtcatt ttccgaacc gatctatac aatctcacc acagggagg aaggaattc agtttcaag aggacgctc aagtacgac tccaagtgg ttggacaaa ccgaacttc gtcggctcg ttcgatatc ggcgattac gttttgttc ttcttcaga gagacagcc gttgaatac atcaactgc ggcaagagc gtctactcg cgagtggcg cgcgtttgc aaaaaggac accggagga aagaatatc ctctcgcaa aactgggcc acttatctc aaagccaga ctcaactgc tccattccg ggagagttt cctttctat ttcaacgaa atacagagc atatacaaa gtgccagga gacgacacc aagttttac ggcaccttc acgacctca accaacggc ttgatgggc tcggcgatc tgctcgttc actctggac gacatccag caggcgttt agcggcaaa ttcaaggaa caggcttcg tcctcgtcc gcctggctg ccggtcttg tcgaatcga gtgccagaa ccgaggcct ggaacgtgt gtcaacgat acggaaacg ctgccagac accgtcctc aattttata agatcgcat ccactcatg gattcagcc gtcatgcac gaacatgag aagcccgtt tttttcaag agagacgtc ttcttcacc agactggtg gtcgataaa atcaaagtg gatatcggg ggcgcagtc ctggactac accgtctac tacgccggg acaaacgaa ggccgcgtg cacaaaatc gtggagtgg caacgcgac accgacggc acgtcgtcc atcctcctg gacgtgttc gacgtgacc cccggcgag cccatccaa gtgatggag atttccaaa gagaacaaa gccctttac gtcgcctcc gactaccgt gttaaacaa gtggaccta gttatgtgc aatcgaagg tacgacaac tgcctgcga tgtgtccac gacccttac tgcggctgg gacaaagac tccaacgtc tgcaaaccg tactcacct ggactattg caagacgtt tccaacagc acagtcgac gtgtgcgac agcagcgtc gttaagaag aaaatgata gtgacgtgg ggccagagc ttgcacttg gggtgcttt ctgaagatg ccggcggtt ttggcctcg cagaccatc acctggtac cactattcg aaggaaaag gggcgctac aagatccag tacaggccg gataagtac attgaaact tccgagcac ggcttggtg atcattgcc gtgactgag gccgacgcc ggccgctat gactgctgg atgggggct tccctgctg tgttcgtac aacgtgaca gtggacgcg caccggtgt tcgcccccg gccaaaagt aacgactat cagaagatt tactcggac tggtgtcac gagttcgag aagtacaaa tcggcgatg aagacttgg gagaagaag caagctcaa tgcgcttcg cgccagaac gacagcaac cagaacagc cacccaaat gaaatctac cggccactg gtctga
- >TC032214 RB
atgaaagag ggttggaaa tccgacatg atcttgatg tcgacagaa gaccagtcc ctcctaaca aggtcggat agatcagag gagcgtgat gcttgtaca aatcatgtg cgagagttc tcttgtggg atgttgtac taccgaacg ttctacgtg gacagcaaa agagattcg ctttacgtt ggagcaatg gatcgtgtc taccgactg aacctcagc aacatcaac caatccagc tgcgagcga gatgccctg aatctggag ccgacgaac gtggcgagc tgcgtttcc aaaggcaaa tcagagcat ttcgactgc cgcaaccac atccgggtt atccaatcg atgggcgat gggagccga ctgtacatt tgtgggacc aacgctcac aaccccaag gactgggtg gttaacgcg aacttgacg catctcagt cggaacacc ttcgtacct ggcattgga atgggcatt gccaaatgc ccctacgat ccagctgac aattcgacg gccgtttgg gtcgaaagg ggaaacccc ggagacttg cccggactt tattccgga accaacgcc gagtttacc aaggcagac actgtcatt ttccgaacc gatctatac aatctcacc acagggagg aaggaattc agtttcaag aggacgctc aagtacgac tccaagtgg ttggacaaa ccgaacttc gtcggctcg ttcgatatc ggcgattac gttttgttc ttcttcaga gagacagcc gttgaatac atcaactgc ggcaagagc gtctactcg cgagtggcg cgcgtttgc aaaaaggac accggagga aagaatatc ctctcgcaa aactgggcc acttatctc aaagccaga ctcaactgc tccattccg ggagagttt cctttctat ttcaacgaa atacagagc atatacaaa gtgccagga gacgacacc aagttttac ggcaccttc acgacctca accaacggc ttgatgggc tcggcgatc tgctcgttc actctggac gacatccag caggcgttt agcggcaaa ttcaaggaa caggcttcg tcctcgtcc gcctggctg ccggtcttg tcgaatcga gtgccagaa ccgaggcct ggaacgtgt gtcaacgat acggaaacg ctgccagac accgtcctc aattttata agatcgcat ccactcatg gattcagcc gtcatgcac gaacatgag aagcccgtt tttttcaag agagacgtc ttcttcacc agactggtg gtcgataaa atcaaagtg gatatcggg ggcgcagtc ctggactac accgtctac tacgccggg acaaacgaa ggccgcgtg cacaaaatc gtggagtgg caacgcgac accgacggc acgtcgtcc atcctcctg gacgtgttc gacgtgacc cccggcgag cccatccaa gtgatggag atttccaaa gagaacaaa gccctttac gtcgcctcc gactaccgt gttaaacaa gtggaccta gttatgtgc aatcgaagg tacgacaac tgcctgcga tgtgtccac gacccttac tgcggctgg gacaaagac tccaacgtc tgcaaaccg tactcacct ggactattg caagacgtt tccaacagc acagtcgac gtgtgcgac agcagcgtc gttaagaag aaaatgata gtgacgtgg ggccagagc ttgcacttg gggtgcttt ctgaagatg ccggcggtt ttggcctcg cagaccatc acctggtac cactattcg aaggaaaag gggcgctac aagatccag tacaggccg gataagtac attgaaact tccgagcac ggcttggtg atcattgcc gtgactgag gccgacgcc ggccgctat gactgctgg atgggggct tccctgctg tgttcgtac aacgtgaca gtggacgcg caccggtgt tcgcccccg gccaaaagt aacgactat cagaagatt tactcggac tggtgtcac gagttcgag aagtacaaa tcggcgatg aagacttgg gagaagaag caagctcaa tgcgcttcg cgccagaac gacagcaac cagaacagc cacccaaat gaaatctac cggccactg gtctga
- >TC032214 RA
ttcgactcg tgatgcctt tggttgagg ttcattcga aagcttccg ccggataca cgtaaacaa tgccgtttt tgagccttt cgtcgccct tttcatgaa cgccgcgct tgtaaatag tcgagtttt gttcagttt ttcagacac cggctcatg aaagcccac tcttgacgc gggtttttt cgtttttcg taacacggg ccacgtgac gaaatggag cgtgctaga gtttccttc ttcaagctg gttattttt gtgactgtg ttcgtggaa atgaagttc gatgtttat gctgatttt gagaacagt tggaccatg ttcatggaa caaccctgt tgttccggc accggaaac caccatgtc aggcatcac agagatcat gtgcgagag ttctcttgt gggatgttg tactaccga acgttctac gtggacagc aaaagagat tcgctttac gttggagca atggatcgt gtctaccga ctgaacctc agcaacatc aaccaatcc agctgcgag cgagatgcc ctgaatctg gagccgacg aacgtggcg agctgcgtt tccaaaggc aaatcagag catttcgac tgccgcaac cacatccgg gttatccaa tcgatgggc gatgggagc cgactgtac atttgtggg accaacgct cacaacccc aaggactgg gtggttaac gcgaacttg acgcatctc agtcggaac accttcgta cctggcatt ggaatgggc attgccaaa tgcccctac gatccagct gacaattcg acggccgtt tgggtcgaa aggggaaac cccggagac ttgcccgga ctttattcc ggaaccaac gccgagttt accaaggca gacactgtc attttccga accgatcta tacaatctc accacaggg aggaaggaa ttcagtttc aagaggacg ctcaagtac gactccaag tggttggac aaaccgaac ttcgtcggc tcgttcgat atcggcgat tacgttttg ttcttcttc agagagaca gccgttgaa tacatcaac tgcggcaag agcgtctac tcgcgagtg gcgcgcgtt tgcaaaaag gacaccgga ggaaagaat atcctctcg caaaactgg gccacttat ctcaaagcc agactcaac tgctccatt ccgggagag tttcctttc tatttcaac gaaatacag agcatatac aaagtgcca ggagacgac accaagttt tacggcacc ttcacgacc tcaaccaac ggcttgatg ggctcggcg atctgctcg ttcactctg gacgacatc cagcaggcg tttagcggc aaattcaag gaacaggct tcgtcctcg tccgcctgg ctgccggtc ttgtcgaat cgagtgcca gaaccgagg cctggaacg tgtgtcaac gatacggaa acgctgcca gacaccgtc ctcaatttt ataagatcg catccactc atggattca gccgtcatg cacgaacat gagaagccc gtttttttc aagagagac gtcttcttc accagactg gtggtcgat aaaatcaaa gtggatatc gggggcgca gtcctggac tacaccgtc tactacgcc gggacaaac gaaggccgc gtgcacaaa atcgtggag tggcaacgc gacaccgac ggcacgtcg tccatcctc ctggacgtg ttcgacgtg acccccggc gagcccatc caagtgatg gagatttcc aaagagaac aaagccctt tacgtcgcc tccgactac cgtgttaaa caagtggac ctagttatg tgcaatcga aggtacgac aactgcctg cgatgtgtc cacgaccct tactgcggc tgggacaaa gactccaac gtctgcaaa ccgtactca cctggacta ttgcaagac gtttccaac agcacagtc gacgtgtgc gacagcagc gtcgttaag aagaaaatg atagtgacg tggggccag agcttgcac ttggggtgc tttctgaag atgccggcg gttttggcc tcgcagacc atcacctgg taccactat tcgaaggaa aaggggcgc tacaagatc cagtacagg ccggataag tacattgaa acttccgag cacggcttg gtgatcatt gccgtgact gaggccgac gccggccgc tatgactgc tggatgggg gcttccctg ctgtgttcg tacaacgtg acagtggac gcgcaccgg tgttcgccc ccggccaaa agtaacgac tatcagaag atttactcg gactggtgt cacgagttc gagaagtac aaatcggcg atgaagact tgggagaag aagcaagct caatgcgct tcgcgccag aacgacagc aaccagaac agccaccca aatgaaatc taccggcca ctggtctga tagctgatc cagcacggt ccacttgcg cttacgttc ttaggaatc ccgttatac tggactctc gttctcttt gtgtaaata gtaccaaag tgaaactac tcaacgatc aatcagtga aagtgccct gtttttgtg atggacgag tgtgaatgg acagtgctt gaagttcgc cctttgttg catttggac gtttcgatg tataaaaag ttttgcttt cccgtttgt tgcgttact atttttgat attgctttg gaaaattct ctaaatatt gtaggtctg tgatatata tgtactgta atttcatgg aggtttctt ttaaggtat aattaatgc aaaacaatc agctcgaaa ctgataact ccggaccaa agtgaatga actgcggtt gaattgtaa ttttagtgc gtattcaaa gcaatactt ttcgcttgc aaagtgttg ggcccttaa aattatata tacccattt gtacatacg ttccagcgc attttttta tgtaaccaa aacagatct tttcgacga accggcaga agatctgtt atgtggatt ttcattcgt atttaagga caatcactt gaacagatt atatatttt cgttccgac atggattca tttgcaagt tacatgata ttgtaaaaa acacatatt gttatcata tttcagaaa atcttatag tactgtttg ttttaataa tattgtact cctgtaaat acctatgta tagttattt ataacatct atgtaattt atactattg tagaaatta caataagac gtttcctgt agctatgct ctagtttta tttcgtgat gcaacaaga ctcagttta tttagttct tttatttca cgacacaat tttttcaat agaaagata catatcttt gtccgcaat aatttaaga gtagtttga cccgttcga tttattgta aaatacgtg ctttacagc tgataattt catccaaaa ttgtagata agtattctt gttacgaat ttaaatagt agagatttt cataactag taaaacttt attcacttc tatgaagtg atataaaat attaataga attagtttt ttctattaa tacttatat cattatttt ggttcgaca taggtttag ttacgtaac aacaaattc tgtgcaaat gtgtttcgt ttcgtgtat tttttgtaa atacctaca ttgcccata agattagtt tacgctata gcagatgac atcctaatg taaattgta atggctatg tcgctattg ctctgaaat tattctcaa taaaaatta tataataaa
- >TC032214 RB
ccggcggcc taggaaatg aaagagggt tggaaatcc gacatgatc ttgatgtcg acagaagac cagtccctc ctaacaagg tcggataga tcagaggag cgtgatgct tgtacaaat catgtgcga gagttctct tgtgggatg ttgtactac cgaacgttc tacgtggac agcaaaaga gattcgctt tacgttgga gcaatggat cgtgtctac cgactgaac ctcagcaac atcaaccaa tccagctgc gagcgagat gccctgaat ctggagccg acgaacgtg gcgagctgc gtttccaaa ggcaaatca gagcatttc gactgccgc aaccacatc cgggttatc caatcgatg ggcgatggg agccgactg tacatttgt gggaccaac gctcacaac cccaaggac tgggtggtt aacgcgaac ttgacgcat ctcagtcgg aacaccttc gtacctggc attggaatg ggcattgcc aaatgcccc tacgatcca gctgacaat tcgacggcc gtttgggtc gaaagggga aaccccgga gacttgccc ggactttat tccggaacc aacgccgag tttaccaag gcagacact gtcattttc cgaaccgat ctatacaat ctcaccaca gggaggaag gaattcagt ttcaagagg acgctcaag tacgactcc aagtggttg gacaaaccg aacttcgtc ggctcgttc gatatcggc gattacgtt ttgttcttc ttcagagag acagccgtt gaatacatc aactgcggc aagagcgtc tactcgcga gtggcgcgc gtttgcaaa aaggacacc ggaggaaag aatatcctc tcgcaaaac tgggccact tatctcaaa gccagactc aactgctcc attccggga gagtttcct ttctatttc aacgaaata cagagcata tacaaagtg ccaggagac gacaccaag ttttacggc accttcacg acctcaacc aacggcttg atgggctcg gcgatctgc tcgttcact ctggacgac atccagcag gcgtttagc ggcaaattc aaggaacag gcttcgtcc tcgtccgcc tggctgccg gtcttgtcg aatcgagtg ccagaaccg aggcctgga acgtgtgtc aacgatacg gaaacgctg ccagacacc gtcctcaat tttataaga tcgcatcca ctcatggat tcagccgtc atgcacgaa catgagaag cccgttttt ttcaagaga gacgtcttc ttcaccaga ctggtggtc gataaaatc aaagtggat atcgggggc gcagtcctg gactacacc gtctactac gccgggaca aacgaaggc cgcgtgcac aaaatcgtg gagtggcaa cgcgacacc gacggcacg tcgtccatc ctcctggac gtgttcgac gtgaccccc ggcgagccc atccaagtg atggagatt tccaaagag aacaaagcc ctttacgtc gcctccgac taccgtgtt aaacaagtg gacctagtt atgtgcaat cgaaggtac gacaactgc ctgcgatgt gtccacgac ccttactgc ggctgggac aaagactcc aacgtctgc aaaccgtac tcacctgga ctattgcaa gacgtttcc aacagcaca gtcgacgtg tgcgacagc agcgtcgtt aagaagaaa atgatagtg acgtggggc cagagcttg cacttgggg tgctttctg aagatgccg gcggttttg gcctcgcag accatcacc tggtaccac tattcgaag gaaaagggg cgctacaag atccagtac aggccggat aagtacatt gaaacttcc gagcacggc ttggtgatc attgccgtg actgaggcc gacgccggc cgctatgac tgctggatg ggggcttcc ctgctgtgt tcgtacaac gtgacagtg gacgcgcac cggtgttcg cccccggcc aaaagtaac gactatcag aagatttac tcggactgg tgtcacgag ttcgagaag tacaaatcg gcgatgaag acttgggag aagaagcaa gctcaatgc gcttcgcgc cagaacgac agcaaccag aacagccac ccaaatgaa atctaccgg ccactggtc tgatagctg atccagcac ggtccactt gcgcttacg ttcttagga atcccgtta tactggact ctcgttctc tttgtgtaa atagtacca aagtgaaac tactcaacg atcaatcag tgaaagtgc cctgttttt gtgatggac gagtgtgaa tggacagtg cttgaagtt cgccctttg ttgcatttg gacgtttcg atgtataaa aagttttgc tttcccgtt tgttgcgtt actattttt gatattgct ttggaaaat tctctaaat attgtaggt ctgtgatat atatgtact gtaatttca tggaggttt cttttaagg tataattaa tgcaaaaca atcagctcg aaactgata actccggac caaagtgaa tgaactgcg gttgaattg taattttag tgcgtattc aaagcaata cttttcgct tgcaaagtg ttgggccct taaaattat atataccca tttgtacat acgttccag cgcattttt ttatgtaac caaaacaga tcttttcga cgaaccggc agaagatct gttatgtgg attttcatt cgtatttaa ggacaatca cttgaacag attatatat tttcgttcc gacatggat tcatttgca agttacatg atattgtaa aaaacacat attgttatc atatttcag aaaatctta tagtactgt ttgttttaa taatattgt actcctgta aatacctat gtatagtta tttataaca tctatgtaa tttatacta ttgtagaaa ttacaataa gacgtttcc tgtagctat gctctagtt ttatttcgt gatgcaaca agactcagt ttatttagt tcttttatt tcacgacac aattttttc aatagaaag atacatatc tttgtccgc aataattta agagtagtt tgacccgtt cgatttatt gtaaaatac gtgctttac agctgataa tttcatcca aaattgtag ataagtatt cttgttacg aatttaaat agtagagat tttcataac tagtaaaac tttattcac ttctatgaa gtgatataa aatattaat agaattagt tttttctat taatactta tatcattat tttggttcg acataggtt tagttacgt aacaacaaa ttctgtgca aatgtgttt cgtttcgtg tattttttg taaatacct acattgccc ataagatta gtttacgct atagcagat gacatccta atgtaaatt gtaatggct atgtcgcta ttgctctga aattattct caataaaaa ttatataat aaa
