Status
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Gene information
General information
TC032214
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001217,TC001219
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 7338986..7444936
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032214
    iBeetle screen iB_00231
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032214 RA
      MERARVSFF KLVIFVTVF VEMKFDVYA DFENSWTMF 
      MEQPCCSGT GNHHVRHHR DHVREFSCG MLYYRTFYV 
      DSKRDSLYV GAMDRVYRL NLSNINQSS CERDALNLE 
      PTNVASCVS KGKSEHFDC RNHIRVIQS MGDGSRLYI 
      CGTNAHNPK DWVVNANLT HLSRNTFVP GIGMGIAKC 
      PYDPADNST AVWVERGNP GDLPGLYSG TNAEFTKAD 
      TVIFRTDLY NLTTGRKEF SFKRTLKYD SKWLDKPNF 
      VGSFDIGDY VLFFFRETA VEYINCGKS VYSRVARVC 
      KKDTGGKNI LSQNWATYL KARLNCSIP GEFPFYFNE 
      IQSIYKVPG DDTKFYGTF TTSTNGLMG SAICSFTLD 
      DIQQAFSGK FKEQASSSS AWLPVLSNR VPEPRPGTC 
      VNDTETLPD TVLNFIRSH PLMDSAVMH EHEKPVFFK 
      RDVFFTRLV VDKIKVDIG GAVLDYTVY YAGTNEGRV 
      HKIVEWQRD TDGTSSILL DVFDVTPGE PIQVMEISK 
      ENKALYVAS DYRVKQVDL VMCNRRYDN CLRCVHDPY 
      CGWDKDSNV CKPYSPGLL QDVSNSTVD VCDSSVVKK 
      KMIVTWGQS LHLGCFLKM PAVLASQTI TWYHYSKEK 
      GRYKIQYRP DKYIETSEH GLVIIAVTE ADAGRYDCW 
      MGASLLCSY NVTVDAHRC SPPAKSNDY QKIYSDWCH 
      EFEKYKSAM KTWEKKQAQ CASRQNDSN QNSHPNEIY 
      RPLV
    • >TC032214 RB
      MKEGWKSDM ILMSTEDQS LLTRSDRSE ERDACTNHV 
      REFSCGMLY YRTFYVDSK RDSLYVGAM DRVYRLNLS 
      NINQSSCER DALNLEPTN VASCVSKGK SEHFDCRNH 
      IRVIQSMGD GSRLYICGT NAHNPKDWV VNANLTHLS 
      RNTFVPGIG MGIAKCPYD PADNSTAVW VERGNPGDL 
      PGLYSGTNA EFTKADTVI FRTDLYNLT TGRKEFSFK 
      RTLKYDSKW LDKPNFVGS FDIGDYVLF FFRETAVEY 
      INCGKSVYS RVARVCKKD TGGKNILSQ NWATYLKAR 
      LNCSIPGEF PFYFNEIQS IYKVPGDDT KFYGTFTTS 
      TNGLMGSAI CSFTLDDIQ QAFSGKFKE QASSSSAWL 
      PVLSNRVPE PRPGTCVND TETLPDTVL NFIRSHPLM 
      DSAVMHEHE KPVFFKRDV FFTRLVVDK IKVDIGGAV 
      LDYTVYYAG TNEGRVHKI VEWQRDTDG TSSILLDVF 
      DVTPGEPIQ VMEISKENK ALYVASDYR VKQVDLVMC 
      NRRYDNCLR CVHDPYCGW DKDSNVCKP YSPGLLQDV 
      SNSTVDVCD SSVVKKKMI VTWGQSLHL GCFLKMPAV 
      LASQTITWY HYSKEKGRY KIQYRPDKY IETSEHGLV 
      IIAVTEADA GRYDCWMGA SLLCSYNVT VDAHRCSPP 
      AKSNDYQKI YSDWCHEFE KYKSAMKTW EKKQAQCAS 
      RQNDSNQNS HPNEIYRPL V
    CDS sequence
    • >TC032214 RA
      atggagcgt gctagagtt tccttcttc aagctggtt 
      atttttgtg actgtgttc gtggaaatg aagttcgat 
      gtttatgct gattttgag aacagttgg accatgttc 
      atggaacaa ccctgttgt tccggcacc ggaaaccac 
      catgtcagg catcacaga gatcatgtg cgagagttc 
      tcttgtggg atgttgtac taccgaacg ttctacgtg 
      gacagcaaa agagattcg ctttacgtt ggagcaatg 
      gatcgtgtc taccgactg aacctcagc aacatcaac 
      caatccagc tgcgagcga gatgccctg aatctggag 
      ccgacgaac gtggcgagc tgcgtttcc aaaggcaaa 
      tcagagcat ttcgactgc cgcaaccac atccgggtt 
      atccaatcg atgggcgat gggagccga ctgtacatt 
      tgtgggacc aacgctcac aaccccaag gactgggtg 
      gttaacgcg aacttgacg catctcagt cggaacacc 
      ttcgtacct ggcattgga atgggcatt gccaaatgc 
      ccctacgat ccagctgac aattcgacg gccgtttgg 
      gtcgaaagg ggaaacccc ggagacttg cccggactt 
      tattccgga accaacgcc gagtttacc aaggcagac 
      actgtcatt ttccgaacc gatctatac aatctcacc 
      acagggagg aaggaattc agtttcaag aggacgctc 
      aagtacgac tccaagtgg ttggacaaa ccgaacttc 
      gtcggctcg ttcgatatc ggcgattac gttttgttc 
      ttcttcaga gagacagcc gttgaatac atcaactgc 
      ggcaagagc gtctactcg cgagtggcg cgcgtttgc 
      aaaaaggac accggagga aagaatatc ctctcgcaa 
      aactgggcc acttatctc aaagccaga ctcaactgc 
      tccattccg ggagagttt cctttctat ttcaacgaa 
      atacagagc atatacaaa gtgccagga gacgacacc 
      aagttttac ggcaccttc acgacctca accaacggc 
      ttgatgggc tcggcgatc tgctcgttc actctggac 
      gacatccag caggcgttt agcggcaaa ttcaaggaa 
      caggcttcg tcctcgtcc gcctggctg ccggtcttg 
      tcgaatcga gtgccagaa ccgaggcct ggaacgtgt 
      gtcaacgat acggaaacg ctgccagac accgtcctc 
      aattttata agatcgcat ccactcatg gattcagcc 
      gtcatgcac gaacatgag aagcccgtt tttttcaag 
      agagacgtc ttcttcacc agactggtg gtcgataaa 
      atcaaagtg gatatcggg ggcgcagtc ctggactac 
      accgtctac tacgccggg acaaacgaa ggccgcgtg 
      cacaaaatc gtggagtgg caacgcgac accgacggc 
      acgtcgtcc atcctcctg gacgtgttc gacgtgacc 
      cccggcgag cccatccaa gtgatggag atttccaaa 
      gagaacaaa gccctttac gtcgcctcc gactaccgt 
      gttaaacaa gtggaccta gttatgtgc aatcgaagg 
      tacgacaac tgcctgcga tgtgtccac gacccttac 
      tgcggctgg gacaaagac tccaacgtc tgcaaaccg 
      tactcacct ggactattg caagacgtt tccaacagc 
      acagtcgac gtgtgcgac agcagcgtc gttaagaag 
      aaaatgata gtgacgtgg ggccagagc ttgcacttg 
      gggtgcttt ctgaagatg ccggcggtt ttggcctcg 
      cagaccatc acctggtac cactattcg aaggaaaag 
      gggcgctac aagatccag tacaggccg gataagtac 
      attgaaact tccgagcac ggcttggtg atcattgcc 
      gtgactgag gccgacgcc ggccgctat gactgctgg 
      atgggggct tccctgctg tgttcgtac aacgtgaca 
      gtggacgcg caccggtgt tcgcccccg gccaaaagt 
      aacgactat cagaagatt tactcggac tggtgtcac 
      gagttcgag aagtacaaa tcggcgatg aagacttgg 
      gagaagaag caagctcaa tgcgcttcg cgccagaac 
      gacagcaac cagaacagc cacccaaat gaaatctac 
      cggccactg gtctga
    • >TC032214 RB
      atgaaagag ggttggaaa tccgacatg atcttgatg 
      tcgacagaa gaccagtcc ctcctaaca aggtcggat 
      agatcagag gagcgtgat gcttgtaca aatcatgtg 
      cgagagttc tcttgtggg atgttgtac taccgaacg 
      ttctacgtg gacagcaaa agagattcg ctttacgtt 
      ggagcaatg gatcgtgtc taccgactg aacctcagc 
      aacatcaac caatccagc tgcgagcga gatgccctg 
      aatctggag ccgacgaac gtggcgagc tgcgtttcc 
      aaaggcaaa tcagagcat ttcgactgc cgcaaccac 
      atccgggtt atccaatcg atgggcgat gggagccga 
      ctgtacatt tgtgggacc aacgctcac aaccccaag 
      gactgggtg gttaacgcg aacttgacg catctcagt 
      cggaacacc ttcgtacct ggcattgga atgggcatt 
      gccaaatgc ccctacgat ccagctgac aattcgacg 
      gccgtttgg gtcgaaagg ggaaacccc ggagacttg 
      cccggactt tattccgga accaacgcc gagtttacc 
      aaggcagac actgtcatt ttccgaacc gatctatac 
      aatctcacc acagggagg aaggaattc agtttcaag 
      aggacgctc aagtacgac tccaagtgg ttggacaaa 
      ccgaacttc gtcggctcg ttcgatatc ggcgattac 
      gttttgttc ttcttcaga gagacagcc gttgaatac 
      atcaactgc ggcaagagc gtctactcg cgagtggcg 
      cgcgtttgc aaaaaggac accggagga aagaatatc 
      ctctcgcaa aactgggcc acttatctc aaagccaga 
      ctcaactgc tccattccg ggagagttt cctttctat 
      ttcaacgaa atacagagc atatacaaa gtgccagga 
      gacgacacc aagttttac ggcaccttc acgacctca 
      accaacggc ttgatgggc tcggcgatc tgctcgttc 
      actctggac gacatccag caggcgttt agcggcaaa 
      ttcaaggaa caggcttcg tcctcgtcc gcctggctg 
      ccggtcttg tcgaatcga gtgccagaa ccgaggcct 
      ggaacgtgt gtcaacgat acggaaacg ctgccagac 
      accgtcctc aattttata agatcgcat ccactcatg 
      gattcagcc gtcatgcac gaacatgag aagcccgtt 
      tttttcaag agagacgtc ttcttcacc agactggtg 
      gtcgataaa atcaaagtg gatatcggg ggcgcagtc 
      ctggactac accgtctac tacgccggg acaaacgaa 
      ggccgcgtg cacaaaatc gtggagtgg caacgcgac 
      accgacggc acgtcgtcc atcctcctg gacgtgttc 
      gacgtgacc cccggcgag cccatccaa gtgatggag 
      atttccaaa gagaacaaa gccctttac gtcgcctcc 
      gactaccgt gttaaacaa gtggaccta gttatgtgc 
      aatcgaagg tacgacaac tgcctgcga tgtgtccac 
      gacccttac tgcggctgg gacaaagac tccaacgtc 
      tgcaaaccg tactcacct ggactattg caagacgtt 
      tccaacagc acagtcgac gtgtgcgac agcagcgtc 
      gttaagaag aaaatgata gtgacgtgg ggccagagc 
      ttgcacttg gggtgcttt ctgaagatg ccggcggtt 
      ttggcctcg cagaccatc acctggtac cactattcg 
      aaggaaaag gggcgctac aagatccag tacaggccg 
      gataagtac attgaaact tccgagcac ggcttggtg 
      atcattgcc gtgactgag gccgacgcc ggccgctat 
      gactgctgg atgggggct tccctgctg tgttcgtac 
      aacgtgaca gtggacgcg caccggtgt tcgcccccg 
      gccaaaagt aacgactat cagaagatt tactcggac 
      tggtgtcac gagttcgag aagtacaaa tcggcgatg 
      aagacttgg gagaagaag caagctcaa tgcgcttcg 
      cgccagaac gacagcaac cagaacagc cacccaaat 
      gaaatctac cggccactg gtctga
    mRNA sequence
    • >TC032214 RA
      ttcgactcg tgatgcctt tggttgagg ttcattcga 
      aagcttccg ccggataca cgtaaacaa tgccgtttt 
      tgagccttt cgtcgccct tttcatgaa cgccgcgct 
      tgtaaatag tcgagtttt gttcagttt ttcagacac 
      cggctcatg aaagcccac tcttgacgc gggtttttt 
      cgtttttcg taacacggg ccacgtgac gaaatggag 
      cgtgctaga gtttccttc ttcaagctg gttattttt 
      gtgactgtg ttcgtggaa atgaagttc gatgtttat 
      gctgatttt gagaacagt tggaccatg ttcatggaa 
      caaccctgt tgttccggc accggaaac caccatgtc 
      aggcatcac agagatcat gtgcgagag ttctcttgt 
      gggatgttg tactaccga acgttctac gtggacagc 
      aaaagagat tcgctttac gttggagca atggatcgt 
      gtctaccga ctgaacctc agcaacatc aaccaatcc 
      agctgcgag cgagatgcc ctgaatctg gagccgacg 
      aacgtggcg agctgcgtt tccaaaggc aaatcagag 
      catttcgac tgccgcaac cacatccgg gttatccaa 
      tcgatgggc gatgggagc cgactgtac atttgtggg 
      accaacgct cacaacccc aaggactgg gtggttaac 
      gcgaacttg acgcatctc agtcggaac accttcgta 
      cctggcatt ggaatgggc attgccaaa tgcccctac 
      gatccagct gacaattcg acggccgtt tgggtcgaa 
      aggggaaac cccggagac ttgcccgga ctttattcc 
      ggaaccaac gccgagttt accaaggca gacactgtc 
      attttccga accgatcta tacaatctc accacaggg 
      aggaaggaa ttcagtttc aagaggacg ctcaagtac 
      gactccaag tggttggac aaaccgaac ttcgtcggc 
      tcgttcgat atcggcgat tacgttttg ttcttcttc 
      agagagaca gccgttgaa tacatcaac tgcggcaag 
      agcgtctac tcgcgagtg gcgcgcgtt tgcaaaaag 
      gacaccgga ggaaagaat atcctctcg caaaactgg 
      gccacttat ctcaaagcc agactcaac tgctccatt 
      ccgggagag tttcctttc tatttcaac gaaatacag 
      agcatatac aaagtgcca ggagacgac accaagttt 
      tacggcacc ttcacgacc tcaaccaac ggcttgatg 
      ggctcggcg atctgctcg ttcactctg gacgacatc 
      cagcaggcg tttagcggc aaattcaag gaacaggct 
      tcgtcctcg tccgcctgg ctgccggtc ttgtcgaat 
      cgagtgcca gaaccgagg cctggaacg tgtgtcaac 
      gatacggaa acgctgcca gacaccgtc ctcaatttt 
      ataagatcg catccactc atggattca gccgtcatg 
      cacgaacat gagaagccc gtttttttc aagagagac 
      gtcttcttc accagactg gtggtcgat aaaatcaaa 
      gtggatatc gggggcgca gtcctggac tacaccgtc 
      tactacgcc gggacaaac gaaggccgc gtgcacaaa 
      atcgtggag tggcaacgc gacaccgac ggcacgtcg 
      tccatcctc ctggacgtg ttcgacgtg acccccggc 
      gagcccatc caagtgatg gagatttcc aaagagaac 
      aaagccctt tacgtcgcc tccgactac cgtgttaaa 
      caagtggac ctagttatg tgcaatcga aggtacgac 
      aactgcctg cgatgtgtc cacgaccct tactgcggc 
      tgggacaaa gactccaac gtctgcaaa ccgtactca 
      cctggacta ttgcaagac gtttccaac agcacagtc 
      gacgtgtgc gacagcagc gtcgttaag aagaaaatg 
      atagtgacg tggggccag agcttgcac ttggggtgc 
      tttctgaag atgccggcg gttttggcc tcgcagacc 
      atcacctgg taccactat tcgaaggaa aaggggcgc 
      tacaagatc cagtacagg ccggataag tacattgaa 
      acttccgag cacggcttg gtgatcatt gccgtgact 
      gaggccgac gccggccgc tatgactgc tggatgggg 
      gcttccctg ctgtgttcg tacaacgtg acagtggac 
      gcgcaccgg tgttcgccc ccggccaaa agtaacgac 
      tatcagaag atttactcg gactggtgt cacgagttc 
      gagaagtac aaatcggcg atgaagact tgggagaag 
      aagcaagct caatgcgct tcgcgccag aacgacagc 
      aaccagaac agccaccca aatgaaatc taccggcca 
      ctggtctga tagctgatc cagcacggt ccacttgcg 
      cttacgttc ttaggaatc ccgttatac tggactctc 
      gttctcttt gtgtaaata gtaccaaag tgaaactac 
      tcaacgatc aatcagtga aagtgccct gtttttgtg 
      atggacgag tgtgaatgg acagtgctt gaagttcgc 
      cctttgttg catttggac gtttcgatg tataaaaag 
      ttttgcttt cccgtttgt tgcgttact atttttgat 
      attgctttg gaaaattct ctaaatatt gtaggtctg 
      tgatatata tgtactgta atttcatgg aggtttctt 
      ttaaggtat aattaatgc aaaacaatc agctcgaaa 
      ctgataact ccggaccaa agtgaatga actgcggtt 
      gaattgtaa ttttagtgc gtattcaaa gcaatactt 
      ttcgcttgc aaagtgttg ggcccttaa aattatata 
      tacccattt gtacatacg ttccagcgc attttttta 
      tgtaaccaa aacagatct tttcgacga accggcaga 
      agatctgtt atgtggatt ttcattcgt atttaagga 
      caatcactt gaacagatt atatatttt cgttccgac 
      atggattca tttgcaagt tacatgata ttgtaaaaa 
      acacatatt gttatcata tttcagaaa atcttatag 
      tactgtttg ttttaataa tattgtact cctgtaaat 
      acctatgta tagttattt ataacatct atgtaattt 
      atactattg tagaaatta caataagac gtttcctgt 
      agctatgct ctagtttta tttcgtgat gcaacaaga 
      ctcagttta tttagttct tttatttca cgacacaat 
      tttttcaat agaaagata catatcttt gtccgcaat 
      aatttaaga gtagtttga cccgttcga tttattgta 
      aaatacgtg ctttacagc tgataattt catccaaaa 
      ttgtagata agtattctt gttacgaat ttaaatagt 
      agagatttt cataactag taaaacttt attcacttc 
      tatgaagtg atataaaat attaataga attagtttt 
      ttctattaa tacttatat cattatttt ggttcgaca 
      taggtttag ttacgtaac aacaaattc tgtgcaaat 
      gtgtttcgt ttcgtgtat tttttgtaa atacctaca 
      ttgcccata agattagtt tacgctata gcagatgac 
      atcctaatg taaattgta atggctatg tcgctattg 
      ctctgaaat tattctcaa taaaaatta tataataaa
    • >TC032214 RB
      ccggcggcc taggaaatg aaagagggt tggaaatcc 
      gacatgatc ttgatgtcg acagaagac cagtccctc 
      ctaacaagg tcggataga tcagaggag cgtgatgct 
      tgtacaaat catgtgcga gagttctct tgtgggatg 
      ttgtactac cgaacgttc tacgtggac agcaaaaga 
      gattcgctt tacgttgga gcaatggat cgtgtctac 
      cgactgaac ctcagcaac atcaaccaa tccagctgc 
      gagcgagat gccctgaat ctggagccg acgaacgtg 
      gcgagctgc gtttccaaa ggcaaatca gagcatttc 
      gactgccgc aaccacatc cgggttatc caatcgatg 
      ggcgatggg agccgactg tacatttgt gggaccaac 
      gctcacaac cccaaggac tgggtggtt aacgcgaac 
      ttgacgcat ctcagtcgg aacaccttc gtacctggc 
      attggaatg ggcattgcc aaatgcccc tacgatcca 
      gctgacaat tcgacggcc gtttgggtc gaaagggga 
      aaccccgga gacttgccc ggactttat tccggaacc 
      aacgccgag tttaccaag gcagacact gtcattttc 
      cgaaccgat ctatacaat ctcaccaca gggaggaag 
      gaattcagt ttcaagagg acgctcaag tacgactcc 
      aagtggttg gacaaaccg aacttcgtc ggctcgttc 
      gatatcggc gattacgtt ttgttcttc ttcagagag 
      acagccgtt gaatacatc aactgcggc aagagcgtc 
      tactcgcga gtggcgcgc gtttgcaaa aaggacacc 
      ggaggaaag aatatcctc tcgcaaaac tgggccact 
      tatctcaaa gccagactc aactgctcc attccggga 
      gagtttcct ttctatttc aacgaaata cagagcata 
      tacaaagtg ccaggagac gacaccaag ttttacggc 
      accttcacg acctcaacc aacggcttg atgggctcg 
      gcgatctgc tcgttcact ctggacgac atccagcag 
      gcgtttagc ggcaaattc aaggaacag gcttcgtcc 
      tcgtccgcc tggctgccg gtcttgtcg aatcgagtg 
      ccagaaccg aggcctgga acgtgtgtc aacgatacg 
      gaaacgctg ccagacacc gtcctcaat tttataaga 
      tcgcatcca ctcatggat tcagccgtc atgcacgaa 
      catgagaag cccgttttt ttcaagaga gacgtcttc 
      ttcaccaga ctggtggtc gataaaatc aaagtggat 
      atcgggggc gcagtcctg gactacacc gtctactac 
      gccgggaca aacgaaggc cgcgtgcac aaaatcgtg 
      gagtggcaa cgcgacacc gacggcacg tcgtccatc 
      ctcctggac gtgttcgac gtgaccccc ggcgagccc 
      atccaagtg atggagatt tccaaagag aacaaagcc 
      ctttacgtc gcctccgac taccgtgtt aaacaagtg 
      gacctagtt atgtgcaat cgaaggtac gacaactgc 
      ctgcgatgt gtccacgac ccttactgc ggctgggac 
      aaagactcc aacgtctgc aaaccgtac tcacctgga 
      ctattgcaa gacgtttcc aacagcaca gtcgacgtg 
      tgcgacagc agcgtcgtt aagaagaaa atgatagtg 
      acgtggggc cagagcttg cacttgggg tgctttctg 
      aagatgccg gcggttttg gcctcgcag accatcacc 
      tggtaccac tattcgaag gaaaagggg cgctacaag 
      atccagtac aggccggat aagtacatt gaaacttcc 
      gagcacggc ttggtgatc attgccgtg actgaggcc 
      gacgccggc cgctatgac tgctggatg ggggcttcc 
      ctgctgtgt tcgtacaac gtgacagtg gacgcgcac 
      cggtgttcg cccccggcc aaaagtaac gactatcag 
      aagatttac tcggactgg tgtcacgag ttcgagaag 
      tacaaatcg gcgatgaag acttgggag aagaagcaa 
      gctcaatgc gcttcgcgc cagaacgac agcaaccag 
      aacagccac ccaaatgaa atctaccgg ccactggtc 
      tgatagctg atccagcac ggtccactt gcgcttacg 
      ttcttagga atcccgtta tactggact ctcgttctc 
      tttgtgtaa atagtacca aagtgaaac tactcaacg 
      atcaatcag tgaaagtgc cctgttttt gtgatggac 
      gagtgtgaa tggacagtg cttgaagtt cgccctttg 
      ttgcatttg gacgtttcg atgtataaa aagttttgc 
      tttcccgtt tgttgcgtt actattttt gatattgct 
      ttggaaaat tctctaaat attgtaggt ctgtgatat 
      atatgtact gtaatttca tggaggttt cttttaagg 
      tataattaa tgcaaaaca atcagctcg aaactgata 
      actccggac caaagtgaa tgaactgcg gttgaattg 
      taattttag tgcgtattc aaagcaata cttttcgct 
      tgcaaagtg ttgggccct taaaattat atataccca 
      tttgtacat acgttccag cgcattttt ttatgtaac 
      caaaacaga tcttttcga cgaaccggc agaagatct 
      gttatgtgg attttcatt cgtatttaa ggacaatca 
      cttgaacag attatatat tttcgttcc gacatggat 
      tcatttgca agttacatg atattgtaa aaaacacat 
      attgttatc atatttcag aaaatctta tagtactgt 
      ttgttttaa taatattgt actcctgta aatacctat 
      gtatagtta tttataaca tctatgtaa tttatacta 
      ttgtagaaa ttacaataa gacgtttcc tgtagctat 
      gctctagtt ttatttcgt gatgcaaca agactcagt 
      ttatttagt tcttttatt tcacgacac aattttttc 
      aatagaaag atacatatc tttgtccgc aataattta 
      agagtagtt tgacccgtt cgatttatt gtaaaatac 
      gtgctttac agctgataa tttcatcca aaattgtag 
      ataagtatt cttgttacg aatttaaat agtagagat 
      tttcataac tagtaaaac tttattcac ttctatgaa 
      gtgatataa aatattaat agaattagt tttttctat 
      taatactta tatcattat tttggttcg acataggtt 
      tagttacgt aacaacaaa ttctgtgca aatgtgttt 
      cgtttcgtg tattttttg taaatacct acattgccc 
      ataagatta gtttacgct atagcagat gacatccta 
      atgtaaatt gtaatggct atgtcgcta ttgctctga 
      aattattct caataaaaa ttatataat aaa