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Gene information
General information
TC001214 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12820399..12821449
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001214
    iBeetle screen iB_00230

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001214 RA
      MDKPASLYV IEGDYGLVS LDVGCIESI FYARLKKLP 
      VEIKLQNGV KSCALYSAP SLRCGNVTV TQFPKIHQH 
      WKSLHGDLD HSLSAKDSS ETLALINLV TMKLNPVLE 
      FLYWVDQRN NEEFISRWF MSALPFPFN YSYTKRKKS 
      EALQLIETL YPLDSDMEV VKEFVTKTA TTCLSTLST 
      RLGKAKFFY GDCPMTVDV VVYAHLAPL VKLPFPTND 
      IPALLSMWP NLTEFVKRI DAKYFPDVK REVKYLQTE 
      SNNRQSDDD VSYLSVFIL VVSATSLVV GFAVKKGFI 
      KLSNFF
    CDS sequence
    • >TC001214 RA
      atggataaa ccggcgagt ctttacgta atcgagggc 
      gattatggc ctggtttcg ctcgacgtc ggttgcatc 
      gagtcgatt ttctacgcc cggctcaag aaactcccc 
      gttgaaata aagctgcaa aacggcgta aaatcatgt 
      gctttgtac agtgcgccc agtctgcgg tgcggcaac 
      gtcacggtg acccagttc ccgaaaatc caccagcac 
      tggaagagc ctccacggc gacctggac cacagcctc 
      tctgccaaa gattccagc gaaacgctc gcgctaata 
      aatctagtc acgatgaaa ctaaacccg gttctggaa 
      ttcctctac tgggtggac cagagaaat aacgaggag 
      ttcatcagt cggtggttc atgagcgcg cttcctttc 
      cccttcaat tactcgtac accaagcgc aaaaaaagc 
      gaggccttg cagctgatc gagaccttg taccccctg 
      gacagcgac atggaggtg gtcaaggag ttcgtcacc 
      aaaacggcc accacgtgt ttgtcaacc ctgtccacg 
      cggttgggc aaagccaag tttttctac ggggattgt 
      cccatgacg gtggacgtt gtggtgtac gcacattta 
      gcccccctt gtcaaattg cccttccct accaatgat 
      attccagcc ttgctgtcc atgtggccg aatctgacc 
      gagtttgtt aaaagaata gacgcgaaa tattttccc 
      gatgttaaa agggaagtc aaatatttg caaactgag 
      agcaataat cggcaaagt gatgatgat gtttcctat 
      ctttccgtt tttatccta gttgtgagt gcgacgtcg 
      cttgtcgtg gggtttgcg gtgaaaaag gggttcatc 
      aaattgtca aattttttt tag
    mRNA sequence
    • >TC001214 RA
      ctgcgctga cagttctcg ttctaacct aaaaaacta 
      gaaattttc gtaatgttt cctgccttg gcttcaaaa 
      tcgtgatta aatggataa accggcgag tctttacgt 
      aatcgaggg cgattatgg cctggtttc gctcgacgt 
      cggttgcat cgagtcgat tttctacgc ccggctcaa 
      gaaactccc cgttgaaat aaagctgca aaacggcgt 
      aaaatcatg tgctttgta cagtgcgcc cagtctgcg 
      gtgcggcaa cgtcacggt gacccagtt cccgaaaat 
      ccaccagca ctggaagag cctccacgg cgacctgga 
      ccacagcct ctctgccaa agattccag cgaaacgct 
      cgcgctaat aaatctagt cacgatgaa actaaaccc 
      ggttctgga attcctcta ctgggtgga ccagagaaa 
      taacgagga gttcatcag tcggtggtt catgagcgc 
      gcttccttt ccccttcaa ttactcgta caccaagcg 
      caaaaaaag cgaggcctt gcagctgat cgagacctt 
      gtaccccct ggacagcga catggaggt ggtcaagga 
      gttcgtcac caaaacggc caccacgtg tttgtcaac 
      cctgtccac gcggttggg caaagccaa gtttttcta 
      cggggattg tcccatgac ggtggacgt tgtggtgta 
      cgcacattt agcccccct tgtcaaatt gcccttccc 
      taccaatga tattccagc cttgctgtc catgtggcc 
      gaatctgac cgagtttgt taaaagaat agacgcgaa 
      atattttcc cgatgttaa aagggaagt caaatattt 
      gcaaactga gagcaataa tcggcaaag tgatgatga 
      tgtttccta tctttccgt ttttatcct agttgtgag 
      tgcgacgtc gcttgtcgt ggggtttgc ggtgaaaaa 
      ggggttcat caaattgtc aaatttttt ttagttgtt 
      cttaagact gaactggct gccagtact gttagtaat 
      tattgtttt tgtaaatgt tttgtaata aagtgatta 
      gaagaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY