Status
Please wait ...
Gene information
General information
TC001209 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12714035..12715581
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001209
    iBeetle screen iB_00228

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    egg chamber potentially segmentation/division not present - Number of animals showing the phenotype: 7

    vitellogenic egg chamber late number increased - Number of animals showing the phenotype: 1

    vitellogenic egg chamber late size decreased - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001209 RA
      MASSAALDS NCITLTRYV LSEQKNAPH ATGELTQLL 
      NSIQTAVKV ISSAVRRAG ITKLFGTVG HTNVQGEEV 
      KKLDCLANE LFINMLSSS YTVALLISE ENETVIEIE 
      TDKRGKYIV AFDPLDGSS NIDCLVSIG SIFAIMKKT 
      DNSIPSVKD ALQPGNQVV AAGYALYGS ATMMVISVG 
      NGVHGFMLD PSVGEFILT DKNMTIPKR GKIYAINEG 
      YTHQWDDAV KEYVSNKKD PSKGKPYNA RYVGSMVAD 
      VHRTIKYGG IFIYPATRD SPNGKLRLL YECIPMAFI 
      MTQAGGLAS NGKIPILDV QPTSLHQRC PIFLGSKED 
      VEEVLAVIK KHSK
    CDS sequence
    • >TC001209 RA
      atggcaagt tcggcggct ttggactcg aattgcatc 
      accctgaca cgatatgtg ttatcagag cagaaaaac 
      gctccccat gcaacaggg gagctcacc caactctta 
      aactccatc caaacagcg gttaaagta attagtagt 
      gctgtacga agggctggg atcacaaaa ttgtttgga 
      acagtgggc cacacaaat gtgcaaggc gaagaggtg 
      aagaaactc gactgcctt gccaacgag ctcttcatc 
      aacatgttg tcctcttcg tacactgtc gctctgctg 
      atctccgaa gagaacgag acagtgatt gaaattgaa 
      actgataaa aggggcaag tacatcgtg gcttttgac 
      ccgctggac ggctcctcc aacatcgat tgcctcgtc 
      tcaatcggt tcaattttc gcaataatg aaaaaaacg 
      gacaattcg atcccgtcg gtgaaggac gccctccag 
      ccgggcaac caggtggta gctgcaggg tacgcgctc 
      tacggcagc gcgaccatg atggtgatc tccgtgggg 
      aacggcgtc cacgggttc atgctggac ccgtccgtg 
      ggcgagttc atcctcact gataagaac atgacgatt 
      cccaaacgg ggcaaaatc tacgccatc aacgagggc 
      tacacccat caatgggac gacgcggtc aaggaatac 
      gtcagtaac aagaaggac ccatcgaag gggaagccg 
      tataatgcg cgatatgtg gggtccatg gtcgccgat 
      gtgcaccgg acgattaaa tacggcggc attttcatt 
      tacccagcg accagggat tcccccaat ggaaagttg 
      cggttgttg tacgaatgc attcccatg gcgtttatt 
      atgacacaa gcgggtggg ttggcttcc aacgggaaa 
      atccccatt ttggacgtg caacccacg agcttgcac 
      cagaggtgt cccattttt ctgggctcg aaggaggat 
      gtggaggag gttctggcc gttattaag aagcactcg 
      aaataa
    mRNA sequence
    • >TC001209 RA
      ttgttcaaa tctccaaat acataactg taaaaggag 
      taattatac aactagtgc ccttttctc cctgaggaa 
      caataattt tgactatta tctcttgga tagacttga 
      ctcagtcgc ttgagaagc ctccacaat ggcaagttc 
      ggcggcttt ggactcgaa ttgcatcac cctgacacg 
      atatgtgtt atcagagca gaaaaacgc tccccatgc 
      aacagggga gctcaccca actcttaaa ctccatcca 
      aacagcggt taaagtaat tagtagtgc tgtacgaag 
      ggctgggat cacaaaatt gtttggaac agtgggcca 
      cacaaatgt gcaaggcga agaggtgaa gaaactcga 
      ctgccttgc caacgagct cttcatcaa catgttgtc 
      ctcttcgta cactgtcgc tctgctgat ctccgaaga 
      gaacgagac agtgattga aattgaaac tgataaaag 
      gggcaagta catcgtggc ttttgaccc gctggacgg 
      ctcctccaa catcgattg cctcgtctc aatcggttc 
      aattttcgc aataatgaa aaaaacgga caattcgat 
      cccgtcggt gaaggacgc cctccagcc gggcaacca 
      ggtggtagc tgcagggta cgcgctcta cggcagcgc 
      gaccatgat ggtgatctc cgtggggaa cggcgtcca 
      cgggttcat gctggaccc gtccgtggg cgagttcat 
      cctcactga taagaacat gacgattcc caaacgggg 
      caaaatcta cgccatcaa cgagggcta cacccatca 
      atgggacga cgcggtcaa ggaatacgt cagtaacaa 
      gaaggaccc atcgaaggg gaagccgta taatgcgcg 
      atatgtggg gtccatggt cgccgatgt gcaccggac 
      gattaaata cggcggcat tttcattta cccagcgac 
      cagggattc ccccaatgg aaagttgcg gttgttgta 
      cgaatgcat tcccatggc gtttattat gacacaagc 
      gggtgggtt ggcttccaa cgggaaaat ccccatttt 
      ggacgtgca acccacgag cttgcacca gaggtgtcc 
      catttttct gggctcgaa ggaggatgt ggaggaggt 
      tctggccgt tattaagaa gcactcgaa ataaatgtt 
      ttgatactc attggattt tttaataaa ttttgttta 
      ttaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY