Status
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Gene information
General information
TC032209
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001204,TC001206,TC001205
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12603298..12663437
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032209
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00227

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    labium not fused - Penetrance (percentage of animals/eggs on slide): more than 80%

    head increased - Penetrance (percentage of animals/eggs on slide): more than 80%

    thorax increased - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen dorsal not closed - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen segment partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen increased - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 83.33% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection



    Pupal morphology
    hindwing slightly position irregular - Number of animals showing the phenotype: 9

    oblique dorsal thoracic muscles slightly shortened - Number of animals showing the phenotype: 6

    oblique dorsal thoracic muscles slightly broader - Number of animals showing the phenotype: 6
    female genital lobes shortened - Number of animals showing the phenotype: 5

    female genital lobes spotted - Number of animals showing the phenotype: 5

    Comment: potentially feces at the developing proctodeum?
    Adult morphology
    wings slightly orientation irregular - Number of animals showing the phenotype: 5


    hindwing potentially folding irregular - Number of animals showing the phenotype: 5

    Fertility
    egg production: number no/almost no eggs -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032209 RA
      MYPSTGMNA AAAAAVAAA RHPGPPQPG QPFKFTVGE 
      SCDRIKEEF NFLQAQYHN LKLECEKLA SEKIEIQRH 
      YVMYYEMSY GLNVEMHKQ TEIAKRLNA IIAQILPFL 
      SQEHQQQVA TAVERAKQV TMTELNAII GQQRPDLPR 
      LLQQMHAQQ LPAHAAPPI PMMPHPGLA GAPPSTAAS 
      LLGLSGALG APGQHPLSM LASKPDLHR PDDVKSNSG 
      INSAEERHR NSISPAERE KYRPRTPQE SEHKKPKKE 
      EKDMGHHSD GEKSDQDLV VDDASEDPV SPPNGTTSP 
      RENGIDKLV PKKEHPGPH SPRSGTSSN ASTPSTKKL 
      DGDKPSTPI SKSVTPTSA AGSSSAASG LKPVVKPPA 
      LGQYPPYLA GMSNGAPHD LQAAAAAGA YSGLHNNLP 
      PALNNYPRP PLQVGYDPH SQMRAPVVP GLGGIPGGK 
      PAYSFHVSA DGQMQPVPF PPDALIGPG IPRHARQIN 
      TLSHGEVVC AVTISNPTK YVYTGGKGC VKVWDISQP 
      GSKSPVSQL DCLQRDNYI RSVKLLPDG RTLIVGGEA 
      SNLSIWDLA SPTPRIKAE LTSSAPACY ALAISPDSK 
      VCFSCCSDG HIAVWDLHN QTLVRQFQG HTDGASCID 
      ISADGTKLW TGGLDNTVR SWDLREGRQ LQQHDFSSQ 
      IFSLGYCPT GEWLAVGME NSNVEVLHA TKPDKYQLH 
      LHESCVLSL RFAACGKWF VSTGKDNLL NAWRTPYGA 
      SIFQSKESS SVLSCDISA DDKYIVTGS GDKKATVYE 
      VIY
    CDS sequence
    • >TC032209 RA
      atgtaccca agtacagga atgaacgcg gcggcagca 
      gcagcggtt gcagccgcg cgccatccg gggcccccg 
      cagcccggc cagccgttc aaattcaca gtgggagaa 
      tcctgcgac aggatcaaa gaagagttt aactttcta 
      caagctcag tatcacaat ttgaaattg gagtgcgag 
      aaactggcg agcgagaaa atcgaaatc cagcgacat 
      tacgtgatg tactacgaa atgtcgtac ggcctgaac 
      gtcgaaatg cataaacag actgaaatc gccaagaga 
      ctgaacgcc atcatcgcg caaatactc cctttcttg 
      tcacaagaa catcaacag caagtcgcg accgctgtt 
      gagagagca aaacaagtt accatgaca gaactcaat 
      gccataata ggacaacag aggccggac ctgccccga 
      ctgctccag cagatgcac gcccagcag ttgccggct 
      cacgcggcg ccccccatc cccatgatg ccgcacccc 
      ggcctcgcc ggggccccc ccgagcacg gccgccagc 
      cttctgggg ctgtcgggg gccttgggg gcccccggc 
      cagcacccc ctgtcgatg ctggcctcc aagcccgac 
      ctgcatcgc cccgacgac gtgaaatcg aacagcggt 
      atcaactcc gccgaagag cgtcaccgg aactcgata 
      tctcctgct gagcgagag aagtaccgc ccccgaaca 
      ccacaagaa tccgaacac aagaagccc aagaaagag 
      gagaaagac atgggacac cacagcgac ggtgagaag 
      agcgaccag gacctggtg gtggacgac gcctccgag 
      gaccccgtg tcgcccccc aacggcacc acgtccccc 
      cgcgagaac ggcatcgat aagctcgtc cccaagaag 
      gagcacccg gggccgcac agcccccgc tccggcacc 
      tccagcaac gcctcgacg ccgtcgacg aagaagctc 
      gacggggat aaaccgtcg acccccatc tcgaaaagc 
      gtcactccc acgtcggcg gcggggtcg tcgtcggcc 
      gccagcggc ctcaaaccg gtggtcaaa ccgccggcg 
      ttgggacaa tacccgccc tacctcgcc ggcatgtcc 
      aatggagcg ccacatgat ctacaagca gctgcagcg 
      gcgggggct tacagcgga ctccataac aatcttcca 
      cctgctttg aataattat ccgcgaccg ccgctgcaa 
      gtcggctac gatccgcac tcgcagatg agggcgccc 
      gtcgtgcca ggactcggg ggcatcccc ggggggaag 
      ccagcttac tcgttccac gttagtgcc gacggccag 
      atgcagccc gttccgttc cctccggac gcgttaata 
      ggtcccgga attccccgt cacgcccgc caaataaac 
      actttgagt catggcgaa gtggtttgt gcagtgaca 
      atatccaac ccaacaaaa tacgtttat accggcggc 
      aaaggctgt gtcaaagtc tgggatata agtcagccc 
      ggatccaag agtccggta tcgcaatta gactgtttg 
      caacgtgat aactacatc cgatctgtg aaactcctc 
      cccgacggg cgcaccctc atcgtcggg ggcgaagcc 
      agcaacctg tccatctgg gacctggcg agccccacg 
      ccccggata aaggccgag ctgacgtcc agcgccccg 
      gcctgttac gcgctggcc atcagcccc gactccaaa 
      gtctgcttc agctgctgt tccgacggc cacatcgcc 
      gtgtgggac ttgcacaac cagacgctg gttcgccag 
      ttccaggga cacaccgac ggggcctcc tgtatcgac 
      atttccgcc gatggcact aagctgtgg acgggaggg 
      ctggataat accgtgcgg agctgggat ttgcgggaa 
      ggaaggcag ttgcaacag cacgacttc agctcgcag 
      atcttctcg ctggggtat tgccccacg ggcgagtgg 
      ctggcggtc gggatggaa aattcgaac gttgaagtg 
      ctacatgcc accaaaccg gacaaatac cagttacat 
      ttgcacgag agttgtgtg ttgagtttg agatttgca 
      gcttgcggg aagtggttt gtctccacg gggaaggat 
      aatctgctg aacgcgtgg aggacgccc tatggggcc 
      agcatattc cagtcgaag gagtcgtcg agtgtgttg 
      agttgtgac atttctgcg gatgataag tacattgtg 
      accggctct ggggacaag aaggcgacg gtgtacgaa 
      gtcatctac tag
    mRNA sequence
    • >TC032209 RA
      ggctttttg taagggagt gtggggtgt gttagcggt 
      tttgggggc gccgctggt gggggaaac cgagtcagt 
      cgtttgcgg accgcggca gcgagcgga cgtgttggt 
      gttgctgtc gaccatcgg cgtacgtcg tcctgtgga 
      tacaagtct gggaagcga ggcggccgc gagtgctgt 
      gagtgtgag tggagagtg ccctttcgg gattacggt 
      gcccaatgg tagtgtacc atgtgttgc tgacgtaag 
      atgtaccca agtacagga atgaacgcg gcggcagca 
      gcagcggtt gcagccgcg cgccatccg gggcccccg 
      cagcccggc cagccgttc aaattcaca gtgggagaa 
      tcctgcgac aggatcaaa gaagagttt aactttcta 
      caagctcag tatcacaat ttgaaattg gagtgcgag 
      aaactggcg agcgagaaa atcgaaatc cagcgacat 
      tacgtgatg tactacgaa atgtcgtac ggcctgaac 
      gtcgaaatg cataaacag actgaaatc gccaagaga 
      ctgaacgcc atcatcgcg caaatactc cctttcttg 
      tcacaagaa catcaacag caagtcgcg accgctgtt 
      gagagagca aaacaagtt accatgaca gaactcaat 
      gccataata ggacaacag aggccggac ctgccccga 
      ctgctccag cagatgcac gcccagcag ttgccggct 
      cacgcggcg ccccccatc cccatgatg ccgcacccc 
      ggcctcgcc ggggccccc ccgagcacg gccgccagc 
      cttctgggg ctgtcgggg gccttgggg gcccccggc 
      cagcacccc ctgtcgatg ctggcctcc aagcccgac 
      ctgcatcgc cccgacgac gtgaaatcg aacagcggt 
      atcaactcc gccgaagag cgtcaccgg aactcgata 
      tctcctgct gagcgagag aagtaccgc ccccgaaca 
      ccacaagaa tccgaacac aagaagccc aagaaagag 
      gagaaagac atgggacac cacagcgac ggtgagaag 
      agcgaccag gacctggtg gtggacgac gcctccgag 
      gaccccgtg tcgcccccc aacggcacc acgtccccc 
      cgcgagaac ggcatcgat aagctcgtc cccaagaag 
      gagcacccg gggccgcac agcccccgc tccggcacc 
      tccagcaac gcctcgacg ccgtcgacg aagaagctc 
      gacggggat aaaccgtcg acccccatc tcgaaaagc 
      gtcactccc acgtcggcg gcggggtcg tcgtcggcc 
      gccagcggc ctcaaaccg gtggtcaaa ccgccggcg 
      ttgggacaa tacccgccc tacctcgcc ggcatgtcc 
      aatggagcg ccacatgat ctacaagca gctgcagcg 
      gcgggggct tacagcgga ctccataac aatcttcca 
      cctgctttg aataattat ccgcgaccg ccgctgcaa 
      gtcggctac gatccgcac tcgcagatg agggcgccc 
      gtcgtgcca ggactcggg ggcatcccc ggggggaag 
      ccagcttac tcgttccac gttagtgcc gacggccag 
      atgcagccc gttccgttc cctccggac gcgttaata 
      ggtcccgga attccccgt cacgcccgc caaataaac 
      actttgagt catggcgaa gtggtttgt gcagtgaca 
      atatccaac ccaacaaaa tacgtttat accggcggc 
      aaaggctgt gtcaaagtc tgggatata agtcagccc 
      ggatccaag agtccggta tcgcaatta gactgtttg 
      caacgtgat aactacatc cgatctgtg aaactcctc 
      cccgacggg cgcaccctc atcgtcggg ggcgaagcc 
      agcaacctg tccatctgg gacctggcg agccccacg 
      ccccggata aaggccgag ctgacgtcc agcgccccg 
      gcctgttac gcgctggcc atcagcccc gactccaaa 
      gtctgcttc agctgctgt tccgacggc cacatcgcc 
      gtgtgggac ttgcacaac cagacgctg gttcgccag 
      ttccaggga cacaccgac ggggcctcc tgtatcgac 
      atttccgcc gatggcact aagctgtgg acgggaggg 
      ctggataat accgtgcgg agctgggat ttgcgggaa 
      ggaaggcag ttgcaacag cacgacttc agctcgcag 
      atcttctcg ctggggtat tgccccacg ggcgagtgg 
      ctggcggtc gggatggaa aattcgaac gttgaagtg 
      ctacatgcc accaaaccg gacaaatac cagttacat 
      ttgcacgag agttgtgtg ttgagtttg agatttgca 
      gcttgcggg aagtggttt gtctccacg gggaaggat 
      aatctgctg aacgcgtgg aggacgccc tatggggcc 
      agcatattc cagtcgaag gagtcgtcg agtgtgttg 
      agttgtgac atttctgcg gatgataag tacattgtg 
      accggctct ggggacaag aaggcgacg gtgtacgaa 
      gtcatctac taggaacaa acgagtggt tttgcacca 
      acttcagtg ccgaacgct gagtccgtg tagtttaaa 
      ttgaagaaa aaaatgtga taattatgt gtaataaac 
      atatgaaga cagtcacag caagattag tgatctgat 
      gtgaaaata ctagatttt attcttaga ttcagtgat 
      ctccatgac aatttgaat gaaccaaga tgcttcatt 
      gtaaataac gattctttg agatgattt acagccacc 
      ttgttgaat gagcgtgtt gttaaatcg acatgaata 
      cttaatttt tatgatttt gagttttcc gtcggggat 
      tgtaaatag caccgataa gagcattgt gcacttgtt 
      tttatttgt tatttaata tgtagcagg agcatgact 
      tgaagaata aaacgaacg tgaacacaa atgtatatt 
      atctatata tttgtaatt aatcctagt gtcgataat 
      aaaatttaa aaagtatct cagtgttgc ctacacaaa 
      tttgctcca attttgcat gtgcaaaag ttagtacct 
      ttgtgtaaa ttaatgtgt ttcggttcg tttagatag 
      catttaata cgtctaaaa tttatgtta gagatttgt 
      agataattg gaaaaatat attattgtt ttttatttt 
      ttaatattt aacatgcaa ttgttttaa tacagatct 
      taattttaa tatctagat atgagacga ataggtaat 
      gctttacct ttctaaaca aatacgttg ttcagtggc 
      gtgtacaag ataacatcg cgcagatta acgtgtcgt 
      gttattatg tacacgtca attcatggt gtgttcatt 
      cacgataca aacttacaa tagaccaac tacatgaac 
      ttttcatac ctcaacaca agcaaacaa tagcccaaa 
      caaatgtat ttttatcat acaattgaa aataaaact 
      tatgttact tg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY