General information | |||
TC032209
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 12603298..12663437 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC032209 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00227 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
remarks: muscle pattern potentially obscured by segmentation defect,
labium not fused - Penetrance (percentage of animals/eggs on slide): more than 80%
head increased - Penetrance (percentage of animals/eggs on slide): more than 80%
thorax increased - Penetrance (percentage of animals/eggs on slide): more than 80%
abdomen dorsal not closed - Penetrance (percentage of animals/eggs on slide): 50% - 80%
abdomen segment partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen increased - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 83.33% (includes death as larva, prepupa, pupa, adult)
oblique dorsal thoracic muscles slightly shortened - Number of animals showing the phenotype: 6
oblique dorsal thoracic muscles slightly broader - Number of animals showing the phenotype: 6
female genital lobes shortened - Number of animals showing the phenotype: 5
female genital lobes spotted - Number of animals showing the phenotype: 5
Comment: potentially feces at the developing proctodeum? |
hindwing potentially folding irregular - Number of animals showing the phenotype: 5
- >TC032209 RA
MYPSTGMNA AAAAAVAAA RHPGPPQPG QPFKFTVGE SCDRIKEEF NFLQAQYHN LKLECEKLA SEKIEIQRH YVMYYEMSY GLNVEMHKQ TEIAKRLNA IIAQILPFL SQEHQQQVA TAVERAKQV TMTELNAII GQQRPDLPR LLQQMHAQQ LPAHAAPPI PMMPHPGLA GAPPSTAAS LLGLSGALG APGQHPLSM LASKPDLHR PDDVKSNSG INSAEERHR NSISPAERE KYRPRTPQE SEHKKPKKE EKDMGHHSD GEKSDQDLV VDDASEDPV SPPNGTTSP RENGIDKLV PKKEHPGPH SPRSGTSSN ASTPSTKKL DGDKPSTPI SKSVTPTSA AGSSSAASG LKPVVKPPA LGQYPPYLA GMSNGAPHD LQAAAAAGA YSGLHNNLP PALNNYPRP PLQVGYDPH SQMRAPVVP GLGGIPGGK PAYSFHVSA DGQMQPVPF PPDALIGPG IPRHARQIN TLSHGEVVC AVTISNPTK YVYTGGKGC VKVWDISQP GSKSPVSQL DCLQRDNYI RSVKLLPDG RTLIVGGEA SNLSIWDLA SPTPRIKAE LTSSAPACY ALAISPDSK VCFSCCSDG HIAVWDLHN QTLVRQFQG HTDGASCID ISADGTKLW TGGLDNTVR SWDLREGRQ LQQHDFSSQ IFSLGYCPT GEWLAVGME NSNVEVLHA TKPDKYQLH LHESCVLSL RFAACGKWF VSTGKDNLL NAWRTPYGA SIFQSKESS SVLSCDISA DDKYIVTGS GDKKATVYE VIY
- >TC032209 RA
atgtaccca agtacagga atgaacgcg gcggcagca gcagcggtt gcagccgcg cgccatccg gggcccccg cagcccggc cagccgttc aaattcaca gtgggagaa tcctgcgac aggatcaaa gaagagttt aactttcta caagctcag tatcacaat ttgaaattg gagtgcgag aaactggcg agcgagaaa atcgaaatc cagcgacat tacgtgatg tactacgaa atgtcgtac ggcctgaac gtcgaaatg cataaacag actgaaatc gccaagaga ctgaacgcc atcatcgcg caaatactc cctttcttg tcacaagaa catcaacag caagtcgcg accgctgtt gagagagca aaacaagtt accatgaca gaactcaat gccataata ggacaacag aggccggac ctgccccga ctgctccag cagatgcac gcccagcag ttgccggct cacgcggcg ccccccatc cccatgatg ccgcacccc ggcctcgcc ggggccccc ccgagcacg gccgccagc cttctgggg ctgtcgggg gccttgggg gcccccggc cagcacccc ctgtcgatg ctggcctcc aagcccgac ctgcatcgc cccgacgac gtgaaatcg aacagcggt atcaactcc gccgaagag cgtcaccgg aactcgata tctcctgct gagcgagag aagtaccgc ccccgaaca ccacaagaa tccgaacac aagaagccc aagaaagag gagaaagac atgggacac cacagcgac ggtgagaag agcgaccag gacctggtg gtggacgac gcctccgag gaccccgtg tcgcccccc aacggcacc acgtccccc cgcgagaac ggcatcgat aagctcgtc cccaagaag gagcacccg gggccgcac agcccccgc tccggcacc tccagcaac gcctcgacg ccgtcgacg aagaagctc gacggggat aaaccgtcg acccccatc tcgaaaagc gtcactccc acgtcggcg gcggggtcg tcgtcggcc gccagcggc ctcaaaccg gtggtcaaa ccgccggcg ttgggacaa tacccgccc tacctcgcc ggcatgtcc aatggagcg ccacatgat ctacaagca gctgcagcg gcgggggct tacagcgga ctccataac aatcttcca cctgctttg aataattat ccgcgaccg ccgctgcaa gtcggctac gatccgcac tcgcagatg agggcgccc gtcgtgcca ggactcggg ggcatcccc ggggggaag ccagcttac tcgttccac gttagtgcc gacggccag atgcagccc gttccgttc cctccggac gcgttaata ggtcccgga attccccgt cacgcccgc caaataaac actttgagt catggcgaa gtggtttgt gcagtgaca atatccaac ccaacaaaa tacgtttat accggcggc aaaggctgt gtcaaagtc tgggatata agtcagccc ggatccaag agtccggta tcgcaatta gactgtttg caacgtgat aactacatc cgatctgtg aaactcctc cccgacggg cgcaccctc atcgtcggg ggcgaagcc agcaacctg tccatctgg gacctggcg agccccacg ccccggata aaggccgag ctgacgtcc agcgccccg gcctgttac gcgctggcc atcagcccc gactccaaa gtctgcttc agctgctgt tccgacggc cacatcgcc gtgtgggac ttgcacaac cagacgctg gttcgccag ttccaggga cacaccgac ggggcctcc tgtatcgac atttccgcc gatggcact aagctgtgg acgggaggg ctggataat accgtgcgg agctgggat ttgcgggaa ggaaggcag ttgcaacag cacgacttc agctcgcag atcttctcg ctggggtat tgccccacg ggcgagtgg ctggcggtc gggatggaa aattcgaac gttgaagtg ctacatgcc accaaaccg gacaaatac cagttacat ttgcacgag agttgtgtg ttgagtttg agatttgca gcttgcggg aagtggttt gtctccacg gggaaggat aatctgctg aacgcgtgg aggacgccc tatggggcc agcatattc cagtcgaag gagtcgtcg agtgtgttg agttgtgac atttctgcg gatgataag tacattgtg accggctct ggggacaag aaggcgacg gtgtacgaa gtcatctac tag
- >TC032209 RA
ggctttttg taagggagt gtggggtgt gttagcggt tttgggggc gccgctggt gggggaaac cgagtcagt cgtttgcgg accgcggca gcgagcgga cgtgttggt gttgctgtc gaccatcgg cgtacgtcg tcctgtgga tacaagtct gggaagcga ggcggccgc gagtgctgt gagtgtgag tggagagtg ccctttcgg gattacggt gcccaatgg tagtgtacc atgtgttgc tgacgtaag atgtaccca agtacagga atgaacgcg gcggcagca gcagcggtt gcagccgcg cgccatccg gggcccccg cagcccggc cagccgttc aaattcaca gtgggagaa tcctgcgac aggatcaaa gaagagttt aactttcta caagctcag tatcacaat ttgaaattg gagtgcgag aaactggcg agcgagaaa atcgaaatc cagcgacat tacgtgatg tactacgaa atgtcgtac ggcctgaac gtcgaaatg cataaacag actgaaatc gccaagaga ctgaacgcc atcatcgcg caaatactc cctttcttg tcacaagaa catcaacag caagtcgcg accgctgtt gagagagca aaacaagtt accatgaca gaactcaat gccataata ggacaacag aggccggac ctgccccga ctgctccag cagatgcac gcccagcag ttgccggct cacgcggcg ccccccatc cccatgatg ccgcacccc ggcctcgcc ggggccccc ccgagcacg gccgccagc cttctgggg ctgtcgggg gccttgggg gcccccggc cagcacccc ctgtcgatg ctggcctcc aagcccgac ctgcatcgc cccgacgac gtgaaatcg aacagcggt atcaactcc gccgaagag cgtcaccgg aactcgata tctcctgct gagcgagag aagtaccgc ccccgaaca ccacaagaa tccgaacac aagaagccc aagaaagag gagaaagac atgggacac cacagcgac ggtgagaag agcgaccag gacctggtg gtggacgac gcctccgag gaccccgtg tcgcccccc aacggcacc acgtccccc cgcgagaac ggcatcgat aagctcgtc cccaagaag gagcacccg gggccgcac agcccccgc tccggcacc tccagcaac gcctcgacg ccgtcgacg aagaagctc gacggggat aaaccgtcg acccccatc tcgaaaagc gtcactccc acgtcggcg gcggggtcg tcgtcggcc gccagcggc ctcaaaccg gtggtcaaa ccgccggcg ttgggacaa tacccgccc tacctcgcc ggcatgtcc aatggagcg ccacatgat ctacaagca gctgcagcg gcgggggct tacagcgga ctccataac aatcttcca cctgctttg aataattat ccgcgaccg ccgctgcaa gtcggctac gatccgcac tcgcagatg agggcgccc gtcgtgcca ggactcggg ggcatcccc ggggggaag ccagcttac tcgttccac gttagtgcc gacggccag atgcagccc gttccgttc cctccggac gcgttaata ggtcccgga attccccgt cacgcccgc caaataaac actttgagt catggcgaa gtggtttgt gcagtgaca atatccaac ccaacaaaa tacgtttat accggcggc aaaggctgt gtcaaagtc tgggatata agtcagccc ggatccaag agtccggta tcgcaatta gactgtttg caacgtgat aactacatc cgatctgtg aaactcctc cccgacggg cgcaccctc atcgtcggg ggcgaagcc agcaacctg tccatctgg gacctggcg agccccacg ccccggata aaggccgag ctgacgtcc agcgccccg gcctgttac gcgctggcc atcagcccc gactccaaa gtctgcttc agctgctgt tccgacggc cacatcgcc gtgtgggac ttgcacaac cagacgctg gttcgccag ttccaggga cacaccgac ggggcctcc tgtatcgac atttccgcc gatggcact aagctgtgg acgggaggg ctggataat accgtgcgg agctgggat ttgcgggaa ggaaggcag ttgcaacag cacgacttc agctcgcag atcttctcg ctggggtat tgccccacg ggcgagtgg ctggcggtc gggatggaa aattcgaac gttgaagtg ctacatgcc accaaaccg gacaaatac cagttacat ttgcacgag agttgtgtg ttgagtttg agatttgca gcttgcggg aagtggttt gtctccacg gggaaggat aatctgctg aacgcgtgg aggacgccc tatggggcc agcatattc cagtcgaag gagtcgtcg agtgtgttg agttgtgac atttctgcg gatgataag tacattgtg accggctct ggggacaag aaggcgacg gtgtacgaa gtcatctac taggaacaa acgagtggt tttgcacca acttcagtg ccgaacgct gagtccgtg tagtttaaa ttgaagaaa aaaatgtga taattatgt gtaataaac atatgaaga cagtcacag caagattag tgatctgat gtgaaaata ctagatttt attcttaga ttcagtgat ctccatgac aatttgaat gaaccaaga tgcttcatt gtaaataac gattctttg agatgattt acagccacc ttgttgaat gagcgtgtt gttaaatcg acatgaata cttaatttt tatgatttt gagttttcc gtcggggat tgtaaatag caccgataa gagcattgt gcacttgtt tttatttgt tatttaata tgtagcagg agcatgact tgaagaata aaacgaacg tgaacacaa atgtatatt atctatata tttgtaatt aatcctagt gtcgataat aaaatttaa aaagtatct cagtgttgc ctacacaaa tttgctcca attttgcat gtgcaaaag ttagtacct ttgtgtaaa ttaatgtgt ttcggttcg tttagatag catttaata cgtctaaaa tttatgtta gagatttgt agataattg gaaaaatat attattgtt ttttatttt ttaatattt aacatgcaa ttgttttaa tacagatct taattttaa tatctagat atgagacga ataggtaat gctttacct ttctaaaca aatacgttg ttcagtggc gtgtacaag ataacatcg cgcagatta acgtgtcgt gttattatg tacacgtca attcatggt gtgttcatt cacgataca aacttacaa tagaccaac tacatgaac ttttcatac ctcaacaca agcaaacaa tagcccaaa caaatgtat ttttatcat acaattgaa aataaaact tatgttact tg
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
