Status
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Gene information
General information
TC001193 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12395958..12400974
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001193
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00226

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 33.33% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Pupal morphology
    wings not closed - Number of animals showing the phenotype: 7

    Adult morphology
    elytra distal not closed - Number of animals showing the phenotype: 5


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001193 RA
      MDVEEQRRL LINQNNPPE NSSEEEWSD ESSDDSYYS 
      DSDSSVPEW ESSIHDTTG ELLFIDCHP FVTQNIDTT 
      KPKTPLQPF SKDQLRRST RGKFLRLIR RRESQRHSR 
      RYPKTPDLD STTNKVKFH DSQEGEEKE VVLKLSRKP 
      ELGLPESLL GISVGTLSD GNRVMVAGF PPDSEAKHE 
      KNIKIGDWL KSVNNIPVT NQNLNSVLD KLSDKHEVL 
      LKLQRVAGV EVTKDPPIN ELNCQSRFV RELVGFGKE 
      DEQLLLEKL AQYPIGILY LNTDKLSEG NPEYDDVIY 
      SYPLPFQKS VLCQSRGIF ITLNHLIND VTRTKPQST 
      SFFHRDKLC HLTYTQENN KLLLFLFPD SCASVKESV 
      LISNEIKRM FLFMYQSLD QCFTIESNR QQINHFFAR 
      CFTRLLNAK KWTTSDYIT GNKALAEPS QCQFEDVLP 
      VAPTLPLPN EAYMQVDDA LTELEASDY REWNEEPLD 
      CQRLFTILG SALYHHGYL LISHLMYED LIDVHVFCR 
      QQGFFHLSK TEPVKTLLL WKEVFPHSC NRSANTNAK 
      IPDGKRYLL LVGSRKDLL AVIMEAGGC TEPAEDNMG 
      PDAFYVEEA QATLAHIQE LGIPELADR WISTNPGPQ 
      VTIPETCTK RKSDFLDTL SKSVSQHKE GRKPEVTSI 
      LKRRNSDQN VTNSSSFFL DDHSDSGSQ CESRRSRCD 
      SDDSDDYGD GSQMSNSSF DISELRQTI LSETGEIQP 
      RQLTAGKDN VLFHFIQFD ATEGILIGP PECRLQSQT 
      IDAILDCFR NACHKFHSL FQNTLRFKN MPAQDMAKS 
      VMNKSLIAI KEHGVMFEC PFLDDIDNK KSNIKYWVV 
      GRLFYMPHP REVYVCYQE NIPQNIIEL AFKIDLDLA 
      K
    CDS sequence
    • >TC001193 RA
      atggatgta gaggaacaa agacgttta ttaataaac 
      caaaataac ccccccgaa aacagttcc gaggaagaa 
      tggtcagat gaatcaagc gatgattct tattactcc 
      gacagcgac agctcggtc cccgaatgg gagtcctca 
      atccacgac acaaccggc gagctcctc ttcatcgac 
      tgccacccc ttcgtcacc caaaacatc gacaccacc 
      aagcccaaa accccctta caacccttc tccaaagac 
      cagctccgg cgcagcacc cgggggaag ttcctccgc 
      ctgatcaga cgccgcgag agccagaga cacagccgc 
      cgctacccc aaaacccca gacctcgac tccaccacc 
      aacaaagtg aagtttcat gactcgcag gagggcgag 
      gagaaagaa gtggtttta aaactgagt cgcaaaccc 
      gagctgggc ctcccagag agtctcctc ggaatcagc 
      gttggcacg ctttcggac ggcaataga gtcatggtt 
      gcggggttt cccccagat agcgaggcc aagcatgag 
      aaaaacata aagatcggc gattggctc aaaagtgtg 
      aacaatatt ccggtgacc aatcagaac ttgaacagc 
      gtgcttgat aagctcagt gataagcat gaggtgctg 
      ttgaagctg caaagagtg gcgggggtg gaagtgact 
      aaagacccg ccaatcaat gaattgaat tgccagtcg 
      cggttcgtg agagaattg gttggcttt ggcaaagaa 
      gacgagcaa ttgttactc gagaaattg gcgcaatat 
      ccaatagga atcctctat ttaaacacg gataaactc 
      agcgaggga aaccccgag tatgatgac gtcatatat 
      tcctaccca ctgccgttc cagaagtcg gtcttgtgc 
      caatcacgc ggcattttt atcacactt aatcacctc 
      attaacgac gttactcgg acaaaaccc cagtcaacg 
      tcgtttttc cacagagat aaactctgc cacctgact 
      tacacccaa gagaacaac aagctactc ctgtttttg 
      tttcccgac agttgcgcc tctgtgaaa gaatcagtc 
      ttgataagc aacgaaatt aaacgaatg tttttattt 
      atgtatcaa agcctggac cagtgtttc acaatcgag 
      tccaaccga caacaaatc aaccatttt ttcgcccgt 
      tgctttacc cgactttta aacgccaaa aagtggaca 
      actagtgac tatatcacc gggaacaag gctttagcc 
      gagcccagt caatgtcaa ttcgaagac gttcttcca 
      gtagccccg actttgccg ttacctaac gaggcatac 
      atgcaagtg gacgacgct ttgaccgaa ctcgaagct 
      agtgattat cgggaatgg aacgaggaa ccgcttgat 
      tgtcaacgc cttttcacg atactcggc agcgccttg 
      taccaccac ggttatttg ctgatctcc catttaatg 
      tacgaggat ttgatcgat gttcatgtt ttttgccgg 
      caacagggg ttcttccac ttgtccaaa accgaaccg 
      gttaagacg ctcctgttg tggaaggag gtttttccg 
      cactcttgc aaccgaagc gcgaacacc aacgccaaa 
      ataccggac ggtaaaaga tacttactg ttggtgggg 
      agcaggaaa gacctctta gctgtgata atggaagcc 
      ggcggttgc acagaaccg gctgaagac aacatgggc 
      ccggacgct ttttacgtg gaagaggcc caagccact 
      ttggcccac atccaagag ctgggaatc cccgagctt 
      gccgatcgg tggatttcg acaaatccc gggccgcag 
      gtgacaatc cccgaaacg tgcaccaag cgcaagtcg 
      gatttcttg gacactttg agcaaatca gtgtcgcaa 
      cataaggaa ggcagaaaa ccggaagtg acttcgatt 
      ttgaagcgg cgtaattcg gaccagaat gttacgaat 
      tcgagctca tttttcctg gatgatcat tcggattcg 
      gggagtcag tgcgagtcg aggaggagt cggtgcgat 
      tccgatgac tcggatgat tacggggat gggagtcag 
      atgagcaac agcagcttt gatatatcg gagttgagg 
      caaacgatt ttgtccgaa actggagag atacagccg 
      agacaattg acggccggg aaagacaat gttttgttc 
      cattttatt cagtttgat gcaactgaa ggcatcttg 
      attggtccg cctgagtgt cggctccag tcgcaaacg 
      attgacgcg attctcgat tgtttcaga aacgcctgt 
      cataagttt cattctttg tttcaaaat acgttgcga 
      ttcaaaaat atgccagcg caagacatg gcgaaatca 
      gtgatgaat aagagtttg attgcaata aaagaacat 
      ggcgttatg tttgaatgt ccttttttg gacgacatc 
      gataataag aagagtaac ataaaatac tgggtcgtt 
      ggaagactg ttttatatg cctcatcca agggaggtc 
      tatgtctgt tatcaggaa aatattcca caaaatatc 
      atcgagttg gcttttaaa attgattta gacctcgcg 
      aaatga
    mRNA sequence
    • >TC001193 RA
      cggtggtaa catttttta cgtatttta taattttaa 
      actttgcaa aatacatca acaaaataa atacgactc 
      gtaatattg ttttaaaca ctaaaatga gttcgttct 
      cgcacgttt gccaccaag agagaaagc catgacctc 
      aggtcactc accatgtat ttaaggtta tgtaactcg 
      aaaaatgtt gaaaacaaa gacggttcc gaagcccta 
      atttagcca caatttttt gtcacaaaa aatggatgt 
      agaggaaca aagacgttt attaataaa ccaaaataa 
      cccccccga aaacagttc cgaggaaga atggtcaga 
      tgaatcaag cgatgattc ttattactc cgacagcga 
      cagctcggt ccccgaatg ggagtcctc aatccacga 
      cacaaccgg cgagctcct cttcatcga ctgccaccc 
      cttcgtcac ccaaaacat cgacaccac caagcccaa 
      aaccccctt acaaccctt ctccaaaga ccagctccg 
      gcgcagcac ccgggggaa gttcctccg cctgatcag 
      acgccgcga gagccagag acacagccg ccgctaccc 
      caaaacccc agacctcga ctccaccac caacaaagt 
      gaagtttca tgactcgca ggagggcga ggagaaaga 
      agtggtttt aaaactgag tcgcaaacc cgagctggg 
      cctcccaga gagtctcct cggaatcag cgttggcac 
      gctttcgga cggcaatag agtcatggt tgcggggtt 
      tcccccaga tagcgaggc caagcatga gaaaaacat 
      aaagatcgg cgattggct caaaagtgt gaacaatat 
      tccggtgac caatcagaa cttgaacag cgtgcttga 
      taagctcag tgataagca tgaggtgct gttgaagct 
      gcaaagagt ggcgggggt ggaagtgac taaagaccc 
      gccaatcaa tgaattgaa ttgccagtc gcggttcgt 
      gagagaatt ggttggctt tggcaaaga agacgagca 
      attgttact cgagaaatt ggcgcaata tccaatagg 
      aatcctcta tttaaacac ggataaact cagcgaggg 
      aaaccccga gtatgatga cgtcatata ttcctaccc 
      actgccgtt ccagaagtc ggtcttgtg ccaatcacg 
      cggcatttt tatcacact taatcacct cattaacga 
      cgttactcg gacaaaacc ccagtcaac gtcgttttt 
      ccacagaga taaactctg ccacctgac ttacaccca 
      agagaacaa caagctact cctgttttt gtttcccga 
      cagttgcgc ctctgtgaa agaatcagt cttgataag 
      caacgaaat taaacgaat gtttttatt tatgtatca 
      aagcctgga ccagtgttt cacaatcga gtccaaccg 
      acaacaaat caaccattt tttcgcccg ttgctttac 
      ccgactttt aaacgccaa aaagtggac aactagtga 
      ctatatcac cgggaacaa ggctttagc cgagcccag 
      tcaatgtca attcgaaga cgttcttcc agtagcccc 
      gactttgcc gttacctaa cgaggcata catgcaagt 
      ggacgacgc tttgaccga actcgaagc tagtgatta 
      tcgggaatg gaacgagga accgcttga ttgtcaacg 
      ccttttcac gatactcgg cagcgcctt gtaccacca 
      cggttattt gctgatctc ccatttaat gtacgagga 
      tttgatcga tgttcatgt tttttgccg gcaacaggg 
      gttcttcca cttgtccaa aaccgaacc ggttaagac 
      gctcctgtt gtggaagga ggtttttcc gcactcttg 
      caaccgaag cgcgaacac caacgccaa aataccgga 
      cggtaaaag atacttact gttggtggg gagcaggaa 
      agacctctt agctgtgat aatggaagc cggcggttg 
      cacagaacc ggctgaaga caacatggg cccggacgc 
      tttttacgt ggaagaggc ccaagccac tttggccca 
      catccaaga gctgggaat ccccgagct tgccgatcg 
      gtggatttc gacaaatcc cgggccgca ggtgacaat 
      ccccgaaac gtgcaccaa gcgcaagtc ggatttctt 
      ggacacttt gagcaaatc agtgtcgca acataagga 
      aggcagaaa accggaagt gacttcgat tttgaagcg 
      gcgtaattc ggaccagaa tgttacgaa ttcgagctc 
      atttttcct ggatgatca ttcggattc ggggagtca 
      gtgcgagtc gaggaggag tcggtgcga ttccgatga 
      ctcggatga ttacgggga tgggagtca gatgagcaa 
      cagcagctt tgatatatc ggagttgag gcaaacgat 
      tttgtccga aactggaga gatacagcc gagacaatt 
      gacggccgg gaaagacaa tgttttgtt ccattttat 
      tcagtttga tgcaactga aggcatctt gattggtcc 
      gcctgagtg tcggctcca gtcgcaaac gattgacgc 
      gattctcga ttgtttcag aaacgcctg tcataagtt 
      tcattcttt gtttcaaaa tacgttgcg attcaaaaa 
      tatgccagc gcaagacat ggcgaaatc agtgatgaa 
      taagagttt gattgcaat aaaagaaca tggcgttat 
      gtttgaatg tcctttttt ggacgacat cgataataa 
      gaagagtaa cataaaata ctgggtcgt tggaagact 
      gttttatat gcctcatcc aagggaggt ctatgtctg 
      ttatcagga aaatattcc acaaaatat catcgagtt 
      ggcttttaa aattgattt agacctcgc gaaatgatt 
      ggctatgct tccctcttt cctttactt tcccttagt 
      aaatttttt aaaactcct ttaatctgg atgatgtga 
      tgcttcctt tacacacac taaacacat aattttaaa 
      aatcccgaa ctcgaaaat ttattttac gtactgatt 
      aactttatc cgaacactc ccgagacaa aataattga 
      attaaattg caaatattt gttgaaata aaaaccttg 
      gctttcttt ttaacttaa aaaaataaa ttcaattga 
      aacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY