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Gene information
General information
TC001189 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 6876585..6880679
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001189
    iBeetle screen iB_00225
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001189 RA
      MNVEQNRLD TFEEWPQDA AVSPPRIAK AGFFYTKHD 
      VTVECFSCH LTISEWNYG DQVMAKHKT LNPSCPFVL 
      NPTTSGNVP IISPTNVPS TSDSMKDEA VRLKTFAKW 
      PKPHIVAPE RLARAGFYY LNTGDNTKC AFCKGVVRA 
      WEPGDDPDQ EHKRHFEDC PFVLTEIVP RLSQNTNGQ 
      VKTDSSSFT NLHLVANEN LHCLGVQTH KGPKKPNYA 
      TLESRLRSF ATWPPDLIQ TPDILSQAG FYYEGMGDQ 
      VRCFHCDGG LRHWDPQDD PWTEHARWF PRCSFIKLV 
      KGQEFVTAC SLELNVNSN LEEVDQKNY PSVQSRKRE 
      VTEQEIQEH MLGEVALSA LSIGLNVER VKRAIREKL 
      EQTGRGYSQ PDALVEAAL NLQHSEEDL SDDQDTHVK 
      NVVENVLDN IIARSCSSE IKTENEPIV KESRSNKTL 
      SLEEENRIL KEARLCKIC MDAEVGIVF LPCGHLTTC 
      VNCAPNLED CPLCRSAIK ATVRTFLS
    CDS sequence
    • >TC001189 RA
      atgaacgtg gaacagaac cgtttggac acgttcgaa 
      gagtggcct caagacgcc gctgtgtca ccccctcga 
      atcgccaaa gccggtttt ttctacacc aagcacgac 
      gtcaccgtc gaatgcttc tcctgccac ttgacaatt 
      tcggaatgg aactacggc gaccaagta atggccaaa 
      cacaagacc ctcaaccct tcttgcccc ttcgtctta 
      aacccaact acctcagga aatgttcct ataatatca 
      cctacaaac gtaccttcc acctccgac tcgatgaag 
      gacgaggcc gtccggctc aaaaccttc gccaagtgg 
      cccaagccc cacatcgtg gcccccgag cgcctcgcc 
      cgcgccggc ttctactat ctcaacacg ggcgacaac 
      accaagtgc gccttctgc aagggggtg gtgcgggcg 
      tgggagccg ggcgacgac cccgaccag gagcacaag 
      cgccacttc gaagactgc cctttcgtc ctcaccgaa 
      atcgtcccc agactcagc caaaacacc aacggacaa 
      gtcaagaca gactcgtcg tctttcacc aatctgcat 
      ctagtcgcc aacgaaaat ttgcactgt ctgggagtc 
      cagacgcac aaaggcccc aaaaaaccc aactacgcc 
      accctcgaa tcgcggctg agatccttc gccacgtgg 
      ccccctgac ctgatccaa accccagat atcctctcc 
      caagccggg ttctactac gagggaatg ggggaccag 
      gtcaggtgc ttccactgc gacggcggc ctcagacac 
      tgggacccc caggacgat ccctggacg gagcacgcc 
      aggtggttc cccaggtgc tccttcatc aagctggtc 
      aaggggcag gaattcgtc accgcctgc tccctcgaa 
      ctcaacgtc aacagcaac ctggaggag gtcgaccag 
      aagaactac ccgagtgtc cagtcgcga aagcgcgag 
      gtcaccgag caagaaatc caggagcat atgttgggg 
      gaagtggcc ctgtcggcc cttagcatc ggcttgaat 
      gtcgaaagg gtgaaaagg gcgattcgg gagaagttg 
      gaacagact ggccgcggc tactcacag ccggacgct 
      ttggtcgaa gccgctctc aatctccag cacagcgag 
      gaggacttg tccgatgat caggacact cacgtgaag 
      aatgtcgta gaaaatgtt ttagataat ataattgca 
      cggagttgt tcgagtgag attaaaacg gaaaacgaa 
      ccgatagtt aaggaatca agatccaat aaaacttta 
      tcgctagaa gaagaaaat cgaattctg aaagaggca 
      agactgtgt aagatttgt atggacgcc gaggtcggt 
      atagtgttt ctgccgtgc ggacattta acgacgtgt 
      gttaattgt gcaccgaat ttggaagat tgccctcta 
      tgtagatcc gccatcaag gccactgtt agaacgttt 
      ttgtcataa
    mRNA sequence
    • >TC001189 RA
      gacgtgtca agattcaaa aatggttga atcgggaga 
      gaattttca agaattgca cgagaaaat cgctgctct 
      ttttgaaaa tttcaaaga agaattgaa tactagaga 
      ccatgatca catgggcta ctttttgta caacgtaaa 
      tatcacctc tgacgtttt gatgtacaa aatcaagta 
      cgaaattga taactttat gagaacgca tgaaaatgt 
      gacagagac aagcgaaag ttctgctgt aaatgcaaa 
      aaagtccga gtgtttgat aaagttgca aactctgac 
      ttcgatgct gacgtgcca cataatgga cgattacag 
      aaagaaaat ggagggaaa aaaatagtt tttgtgaat 
      aaatttgta ataattatg taaattaga aaaaatcgt 
      ttgaattct aatgacttt ggcatttgg gtcggtaaa 
      agcggtccc tagccggtg tttgagtaa tgtcagctg 
      atagctgac agtccttgg tgaagtggt tcgaggagg 
      aacggttcc catgtctta gacttagta tattggtgt 
      ttctgccgt gctacttaa tagcgaggt tctcaaagt 
      cggcgctga gctcgattt aaaaaaatg tcataacgt 
      gaaaatcgc gaaaatgaa cgtggaaca gaaccgttt 
      ggacacgtt cgaagagtg gcctcaaga cgccgctgt 
      gtcaccccc tcgaatcgc caaagccgg ttttttcta 
      caccaagca cgacgtcac cgtcgaatg cttctcctg 
      ccacttgac aatttcgga atggaacta cggcgacca 
      agtaatggc caaacacaa gaccctcaa cccttcttg 
      ccccttcgt cttaaaccc aactacctc aggaaatgt 
      tcctataat atcacctac aaacgtacc ttccacctc 
      cgactcgat gaaggacga ggccgtccg gctcaaaac 
      cttcgccaa gtggcccaa gccccacat cgtggcccc 
      cgagcgcct cgcccgcgc cggcttcta ctatctcaa 
      cacgggcga caacaccaa gtgcgcctt ctgcaaggg 
      ggtggtgcg ggcgtggga gccgggcga cgaccccga 
      ccaggagca caagcgcca cttcgaaga ctgcccttt 
      cgtcctcac cgaaatcgt ccccagact cagccaaaa 
      caccaacgg acaagtcaa gacagactc gtcgtcttt 
      caccaatct gcatctagt cgccaacga aaatttgca 
      ctgtctggg agtccagac gcacaaagg ccccaaaaa 
      acccaacta cgccaccct cgaatcgcg gctgagatc 
      cttcgccac gtggccccc tgacctgat ccaaacccc 
      agatatcct ctcccaagc cgggttcta ctacgaggg 
      aatggggga ccaggtcag gtgcttcca ctgcgacgg 
      cggcctcag acactggga cccccagga cgatccctg 
      gacggagca cgccaggtg gttccccag gtgctcctt 
      catcaagct ggtcaaggg gcaggaatt cgtcaccgc 
      ctgctccct cgaactcaa cgtcaacag caacctgga 
      ggaggtcga ccagaagaa ctacccgag tgtccagtc 
      gcgaaagcg cgaggtcac cgagcaaga aatccagga 
      gcatatgtt gggggaagt ggccctgtc ggcccttag 
      catcggctt gaatgtcga aagggtgaa aagggcgat 
      tcgggagaa gttggaaca gactggccg cggctactc 
      acagccgga cgctttggt cgaagccgc tctcaatct 
      ccagcacag cgaggagga cttgtccga tgatcagga 
      cactcacgt gaagaatgt cgtagaaaa tgttttaga 
      taatataat tgcacggag ttgttcgag tgagattaa 
      aacggaaaa cgaaccgat agttaagga atcaagatc 
      caataaaac tttatcgct agaagaaga aaatcgaat 
      tctgaaaga ggcaagact gtgtaagat ttgtatgga 
      cgccgaggt cggtatagt gtttctgcc gtgcggaca 
      tttaacgac gtgtgttaa ttgtgcacc gaatttgga 
      agattgccc tctatgtag atccgccat caaggccac 
      tgttagaac gtttttgtc ataactatt tattaaact 
      caataaatt tattttata acacgactg ctttcattc 
      aactccaaa gcctttgtt tcctatcgg tgataacat 
      atcacagcg gaataaaac cgccaaact ggatcaatc 
      aatccagtt atggccccg gaccgccca gttatgtgc 
      agcgccgga attggcaat ctctcaatt tgaaaagtg 
      agt