Status
Please wait ...
Gene information
General information
TC032207
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001187
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 12354788..12356512
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032207
    iBeetle screen iB_00224

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032207 RA
      MINTIILLS LCFRFGTTY PKEFARRCG HVHLTISAS 
      QDTNLELNW ITDCTPPDN IRPDYIALY HYNLKDRSE 
      ETPPLVKIR ASDHPSGYY KTKIKFGQP WLPGNWEYR 
      DSLTRADQG PHCYPYWIA SIKGNDIID TRCLGIQPT 
      WMNDNRMQI GTQKIGNLF IPGTHNSGA YVGVPKILE 
      NYILNQDRS IWTQLVHGI RYLDLRIGY YENDGFYVN 
      HDLVRITKV VQIFKEIRK FVQLAPKEI IVVDFHRFP 
      YPSNFNSTL HEKFVSLVY DYLGDLALP PGGLQVGKG 
      PTLNEIWAQ NKNVIICYA DKAIARENY WLWQPLYQH 
      WANTKNVDT LKSFLSRAI KEHRVTLNP MFALMAELT 
      PQPIDLFFR TNNLRKLAN DVNRKVTVW FRDEWARDV 
      NIVATDYFL GNDIINVAI EANNNR
    CDS sequence
    • >TC032207 RA
      atgatcaac accattatt ttattatcg ctgtgtttc 
      cgtttcggg accacttat cccaaggaa ttcgcgcga 
      agatgcggc cacgtccac ctgacaatc tcagcgtcc 
      caagacacg aatttagaa ctcaactgg atcactgat 
      tgcactcct cctgataac atcaggcca gattacatc 
      gccttgtac cattacaat ttaaaagac cgaagcgag 
      gaaacgccg ccactcgtc aaaatacgc gcctctgac 
      catccctcg ggatactac aagaccaaa atcaagttc 
      ggccagccc tggctgccc gggaactgg gaatacagg 
      gacagtctg acccgagcc gaccaaggc ccgcattgc 
      tatccctac tggatcgcg tcaattaaa gggaacgat 
      atcattgat accagatgt ctggggata caaccaacc 
      tggatgaat gataatagg atgcaaatt gggactcaa 
      aaaatcggc aacctgttc attccaggg actcacaac 
      tcgggcgcc tacgtgggc gtgccaaaa attttggaa 
      aactatatt ctgaaccaa gatcgcagc atctggacc 
      caattggtg catggaatc cgttacctc gacttacga 
      atcggctat tacgaaaat gacggtttt tacgtgaac 
      cacgacctc gtccggata actaaagtg gtccagatt 
      tttaaagag atcaggaag ttcgtccaa cttgcgccc 
      aaagaaatc atagtcgtt gatttccac cgttttccc 
      tacccttca aacttcaac tcgactttg cacgaaaaa 
      ttcgtatcc ctggtgtat gactatttg ggcgacttg 
      gccctgcct cctggcggg ctccaggtg ggcaaaggg 
      ccaacgctg aacgagatc tgggcccag aacaagaac 
      gtgatcatt tgctacgcc gataaggcc atagcccga 
      gaaaattac tggttgtgg caacccttg taccagcac 
      tgggccaat acgaaaaac gtggacacg ttgaagagt 
      ttcctctcg cgggccatc aaagaacac agagtgacc 
      ctaaacccg atgttcgcc ctcatggct gaactgacc 
      ccccaaccc atcgatctg ttcttccgc actaacaac 
      ctgcggaag cttgccaac gacgttaac cggaaggtt 
      acggtttgg tttcgcgat gagtgggcc agggatgtt 
      aatatagtg gcgaccgac tattttttg ggcaacgat 
      attataaac gtggccatt gaggcgaac aacaaccgg 
      tag
    mRNA sequence
    • >TC032207 RA
      gtggcaata actttgttt atagtgagt ctttgtggt 
      tacaaatga acattccga tcgacttta ccttcgtat 
      taatttaat cattgacca ggcgaaagt ccaatgatc 
      aacaccatt attttatta tcgctgtgt ttccgtttc 
      gggaccact tatcccaag gaattcgcg cgaagatgc 
      ggccacgtc cacctgaca atctcagcg tcccaagac 
      acgaattta gaactcaac tggatcact gattgcact 
      cctcctgat aacatcagg ccagattac atcgccttg 
      taccattac aatttaaaa gaccgaagc gaggaaacg 
      ccgccactc gtcaaaata cgcgcctct gaccatccc 
      tcgggatac tacaagacc aaaatcaag ttcggccag 
      ccctggctg cccgggaac tgggaatac agggacagt 
      ctgacccga gccgaccaa ggcccgcat tgctatccc 
      tactggatc gcgtcaatt aaagggaac gatatcatt 
      gataccaga tgtctgggg atacaacca acctggatg 
      aatgataat aggatgcaa attgggact caaaaaatc 
      ggcaacctg ttcattcca gggactcac aactcgggc 
      gcctacgtg ggcgtgcca aaaattttg gaaaactat 
      attctgaac caagatcgc agcatctgg acccaattg 
      gtgcatgga atccgttac ctcgactta cgaatcggc 
      tattacgaa aatgacggt ttttacgtg aaccacgac 
      ctcgtccgg ataactaaa gtggtccag atttttaaa 
      gagatcagg aagttcgtc caacttgcg cccaaagaa 
      atcatagtc gttgatttc caccgtttt ccctaccct 
      tcaaacttc aactcgact ttgcacgaa aaattcgta 
      tccctggtg tatgactat ttgggcgac ttggccctg 
      cctcctggc gggctccag gtgggcaaa gggccaacg 
      ctgaacgag atctgggcc cagaacaag aacgtgatc 
      atttgctac gccgataag gccatagcc cgagaaaat 
      tactggttg tggcaaccc ttgtaccag cactgggcc 
      aatacgaaa aacgtggac acgttgaag agtttcctc 
      tcgcgggcc atcaaagaa cacagagtg accctaaac 
      ccgatgttc gccctcatg gctgaactg accccccaa 
      cccatcgat ctgttcttc cgcactaac aacctgcgg 
      aagcttgcc aacgacgtt aaccggaag gttacggtt 
      tggtttcgc gatgagtgg gccagggat gttaatata 
      gtggcgacc gactatttt ttgggcaac gatattata 
      aacgtggcc attgaggcg aacaacaac cggtaggac 
      ggtgggaga gggatccga gctcaggga gtggaggcg 
      agaatatct atccaactt aatcctctt ttgtaatcc 
      aataaaacg cgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY