Status
Please wait ...
Gene information
General information
TC032187
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001150
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11890371..11893253
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032187
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00219

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032187 RA
      MNSNKFFED YEKLKTVGQ GAFGTATLY RRLNDGKQV 
      VMKEVFLMD MDNSEKKSA LNEVEILSN LNHPNIIKY 
      LGSFQKEGS LVIIMEYAD GGNLAQLIN AKKHKNEIF 
      TEKSILNIL SQISAAISY MHTNKILHR DLKSANIFL 
      NMNGNVKVG DFGISKMLN TRSQAQTVV GTPYYLSPE 
      MCEGSDYNE KSDIWAIGC ILYELACLR KPFEAMTLP 
      VLVQKITAC EYSNIPEIY SDELSQLVH SILQRDPVA 
      RPSAKYIHD TIVPKTAKK FEAQSAKIS VSRERSILY 
      KINNFGDDQ QIIPQELPH KTIIDFSIG YSHSIVVTI 
      DHCVHTWGE NDFGQLGIN NTQKQTFAT IVKSLQHEK 
      IIRCAAGRN FSLFLNAEG VVFSCGDAQ QGCLGHGNW 
      DSVQSPTII EALMHEKIA KISCGDEHV VALSTEGRV 
      FTWGSSKNG QLGQGHMQY RCSPLAIKL LNLTKIKDI 
      FCGENETVL LTINGQVLV SGLNNYRKL GVADQEKIY 
      LFTPVPIRE KIKFVSIGL NHTMMLTEN GVLIALGRN 
      SEGQLGKGN FINYPQPQT IKLRNKITM IACGLNFTV 
      AADNRNVVW FWGSSYKTY KNMPHKNSN SVLPLSKNR 
      GEDTMIIKD FGIEESLDQ FDSQCAKIP RSILAFYAS 
      PSNIKTGNV IYVGEIKIK AGNLYVLAH TTTPPPVKQ 
      FTDRDINKD TMSQIGGTT AAPTTLTSL PNMV
    CDS sequence
    • >TC032187 RA
      atgaacagc aacaagttt ttcgaagat tacgaaaaa 
      ctcaagact gtagggcaa ggagctttt ggtaccgcg 
      acactgtat cgacgttta aatgatggg aaacaggtc 
      gtcatgaaa gaggttttc ctgatggat atggacaat 
      tcggaaaaa aaatcagcc ttgaacgaa gttgagatt 
      ttatcaaac ttgaatcat cccaatatt atcaaatat 
      cttgggagt tttcaaaag gaaggttcg ttagtcata 
      ataatggaa tacgccgat ggtggtaac ttggcgcag 
      ctcattaac gcaaagaaa cacaaaaat gagattttc 
      accgagaaa agcatccta aacattctg tcacaaatc 
      agtgccgct attagctac atgcacacg aacaaaatt 
      ttacacaga gacttgaaa tcggcaaac atatttttg 
      aacatgaac ggtaacgta aaagtgggc gattttggc 
      atttctaaa atgctcaat acccgatca caagcgcaa 
      actgtcgtc ggcacccct tactacctc agtcccgaa 
      atgtgcgaa gggtctgac tataacgaa aagtccgac 
      atttgggca atcggctgc attctgtac gagctcgca 
      tgtttgagg aaaccgttc gaagcgatg actttacca 
      gtcctcgtc caaaaaatc acagcgtgc gaatattcc 
      aacattcct gaaatatat tcggacgag ttgtcgcaa 
      ctcgtccac tcgattctg caaagagac ccagttgcg 
      agaccctca gcgaaatac atccacgat acgattgta 
      ccaaaaaca gcgaaaaaa tttgaagca caaagcgcc 
      aaaataagt gtttcacga gaacgctcg atcctctac 
      aaaatcaac aatttcggc gacgaccaa caaataatc 
      ccccaggag ttgccccac aaaacaatc atagatttc 
      tcgatcggc tatagtcac agtattgtt gttacgatc 
      gatcactgt gtgcacact tggggcgaa aatgatttc 
      ggccagctt gggataaac aatacgcag aaacaaaca 
      ttcgccaca atagtaaaa tccttgcag cacgaaaaa 
      attattcga tgcgcggca ggacgaaac tttagtctt 
      tttttgaat gccgaaggg gttgtgttt agttgtggg 
      gacgcacag caagggtgc ttgggccat ggtaattgg 
      gattcggtg caaagtccc acaataata gaagcacta 
      atgcacgaa aagatcgcc aaaatatcc tgcggtgac 
      gaacacgtg gttgcgcta tcaaccgag ggcagagtg 
      ttcacctgg ggctcctca aaaaacggc caacttggc 
      caaggccac atgcaatac agatgttct cctctggct 
      ataaaactt ctcaatttg acgaagata aaagacata 
      ttttgcggg gaaaacgaa actgtttta ttaacaatc 
      aacggccaa gttctagtg agtggattg aacaattac 
      cgcaaactt ggagtcgca gatcaggaa aaaatttat 
      ttattcact cctgtgccg atcagagaa aaaattaaa 
      ttcgtctcc attggttta aaccacacg atgatgcta 
      acggaaaac ggggtccta attgccttg gggcgaaat 
      tccgaaggg cagttagga aaaggaaat ttcattaac 
      tacccccag ccacaaaca attaaatta cgaaataaa 
      attacaatg atcgcgtgc ggtttgaac tttactgtg 
      gcggccgat aatcgaaat gttgtttgg ttttggggt 
      tcttcgtac aaaacgtat aaaaacatg ccgcacaaa 
      aacagcaac tctgttctg cctttgtcc aaaaatcga 
      ggcgaagac acgatgata attaaagat tttgggatt 
      gaagagagt ttggatcag tttgactca caatgtgcg 
      aaaattccg cgctccata ttggcgttt tatgcgtct 
      ccaagtaat attaaaaca gggaatgtt atttatgtg 
      ggtgaaata aaaatcaaa gcgggtaat ttgtacgtg 
      ctagcacac actaccacg cctccgcct gtcaaacag 
      ttcactgat agagatatt aataaagac acaatgtca 
      caaattgga ggtactaca gctgcgccc actacgcta 
      acaagtttg ccaaacatg gtttaa
    mRNA sequence
    • >TC032187 RA
      ctacgtaaa aatgaacag caacaagtt tttcgaaga 
      ttacgaaaa actcaagac tgtagggca aggagcttt 
      tggtaccgc gacactgta tcgacgttt aaatgatgg 
      gaaacaggt cgtcatgaa agaggtttt cctgatgga 
      tatggacaa ttcggaaaa aaaatcagc cttgaacga 
      agttgagat tttatcaaa cttgaatca tcccaatat 
      tatcaaata tcttgggag ttttcaaaa ggaaggttc 
      gttagtcat aataatgga atacgccga tggtggtaa 
      cttggcgca gctcattaa cgcaaagaa acacaaaaa 
      tgagatttt caccgagaa aagcatcct aaacattct 
      gtcacaaat cagtgccgc tattagcta catgcacac 
      gaacaaaat tttacacag agacttgaa atcggcaaa 
      catattttt gaacatgaa cggtaacgt aaaagtggg 
      cgattttgg catttctaa aatgctcaa tacccgatc 
      acaagcgca aactgtcgt cggcacccc ttactacct 
      cagtcccga aatgtgcga agggtctga ctataacga 
      aaagtccga catttgggc aatcggctg cattctgta 
      cgagctcgc atgtttgag gaaaccgtt cgaagcgat 
      gactttacc agtcctcgt ccaaaaaat cacagcgtg 
      cgaatattc caacattcc tgaaatata ttcggacga 
      gttgtcgca actcgtcca ctcgattct gcaaagaga 
      cccagttgc gagaccctc agcgaaata catccacga 
      tacgattgt accaaaaac agcgaaaaa atttgaagc 
      acaaagcgc caaaataag tgtttcacg agaacgctc 
      gatcctcta caaaatcaa caatttcgg cgacgacca 
      acaaataat cccccagga gttgcccca caaaacaat 
      catagattt ctcgatcgg ctatagtca cagtattgt 
      tgttacgat cgatcactg tgtgcacac ttggggcga 
      aaatgattt cggccagct tgggataaa caatacgca 
      gaaacaaac attcgccac aatagtaaa atccttgca 
      gcacgaaaa aattattcg atgcgcggc aggacgaaa 
      ctttagtct ttttttgaa tgccgaagg ggttgtgtt 
      tagttgtgg ggacgcaca gcaagggtg cttgggcca 
      tggtaattg ggattcggt gcaaagtcc cacaataat 
      agaagcact aatgcacga aaagatcgc caaaatatc 
      ctgcggtga cgaacacgt ggttgcgct atcaaccga 
      gggcagagt gttcacctg gggctcctc aaaaaacgg 
      ccaacttgg ccaaggcca catgcaata cagatgttc 
      tcctctggc tataaaact tctcaattt gacgaagat 
      aaaagacat attttgcgg ggaaaacga aactgtttt 
      attaacaat caacggcca agttctagt gagtggatt 
      gaacaatta ccgcaaact tggagtcgc agatcagga 
      aaaaattta tttattcac tcctgtgcc gatcagaga 
      aaaaattaa attcgtctc cattggttt aaaccacac 
      gatgatgct aacggaaaa cggggtcct aattgcctt 
      ggggcgaaa ttccgaagg gcagttagg aaaaggaaa 
      tttcattaa ctaccccca gccacaaac aattaaatt 
      acgaaataa aattacaat gatcgcgtg cggtttgaa 
      ctttactgt ggcggccga taatcgaaa tgttgtttg 
      gttttgggg ttcttcgta caaaacgta taaaaacat 
      gccgcacaa aaacagcaa ctctgttct gcctttgtc 
      caaaaatcg aggcgaaga cacgatgat aattaaaga 
      ttttgggat tgaagagag tttggatca gtttgactc 
      acaatgtgc gaaaattcc gcgctccat attggcgtt 
      ttatgcgtc tccaagtaa tattaaaac agggaatgt 
      tatttatgt gggtgaaat aaaaatcaa agcgggtaa 
      tttgtacgt gctagcaca cactaccac gcctccgcc 
      tgtcaaaca gttcactga tagagatat taataaaga 
      cacaatgtc acaaattgg aggtactac agctgcgcc 
      cactacgct aacaagttt gccaaacat ggtttaata 
      tttcagtaa aaagtacag catatacag ttgttaata 
      aattaattg tcgtagtta atgccttta ccctttttg 
      gtgacggtt tttgaggaa taaactttt cttttacgt 
      tttatatga ttccaagga tcctgcttc acactgaaa 
      ttaaaaaca attttacta g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY