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Gene information
General information
TC001142 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 6016789..6020670
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001142
    iBeetle screen iB_00218
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    remarks: animals lost,
    Adult morphology
    remarks: animals lost,
    Fertility
    egg chamber mostly not present - Number of animals showing the phenotype: 6

    Analysis of embryonic musculature and early development
    remarks: animals lost, (egglay)
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 55.56% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection



    pupal molt not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection



    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001142 RA
      MVVGSDGRF AYTNHLFNP YEREHWWIP MDHCYARPW 
      NWRPESTFL RPTKTLFMP KVPPGRKKA INPLAPIQE 
      CDDVIDVVT DSEEPTPIY DKAKAKHLM EECEKHAAM 
      ARVDEGNED WEESVSRLN WNTTQNRLF NGVVNILNA 
      DHLARLAHS DSSNEAIAR RVTIDKSVE RMRRLMATV 
      SWDPKYTQW LHQILIDHL STSYLAAYL DILQTLRSK 
      LPTFVDKMM GAANSSKLS ILSYETLFP LLKRPWDPV 
      ASSLMQDKP KKLPGNPVI VVVPSTPIS SKRMLKWIN 
      LLSNLATVI TVPANYGGG LHKTTMMNC VDQMFVITR 
      NKIQDIRLD YPGRSIVLA GFGFGATLA LQIAQVEQV 
      LCVISIGFS LLTADGKRG EVDDNLLEL QCPILFVIG 
      QCSNTSLQE DMEDLRERM RVETGLIVV GNADDNLRV 
      SKKKKKAEG ITQSIVDRC VADEIGEFI SGVILSPYP 
      PQLRQSPTN LTTEGLVSK KLKSERKRY NSNTSSIDS 
      EPPSPTPRI SRPGQNSSH LGRPPGSKS KAKMEAKWA 
      AQGIPSSPS NSPPHLSNT PDTSSNDSI LTDKLSLTN 
      FDPSPVVKK LKTLKPTVV SPEKTTTNQ HTSTSNQNT 
      AKAMGLQNR LSGLLQGDV STLIQPSMV KSNSSGIKE 
      NVKITGGLN PKFFSNNGK MVDMSKVSV INPKSNNAN 
      SLVLLPDGK IKSYAPSVK VPGGRYITS KRQLLGNKP 
      PKTVKKVSY ISQPMQTNL SPPTNLTTQ DIMNLPIIF 
      ADDNQILDN TLTQPEMKP VPSSPNSKL SANTGSKFM 
      LINNKPSNF IISPNPKPS VSQPAKATP KYTKIILSS 
      KQTATSNVV KKITNLPSE ISVRKVTPS ETATISQSE 
      ELDLENEIA ASTLYKRKV ESTDAECLE IVSNKPEEE 
      KLEGESVKR TAEEANIDN DESTPCKVS KVETA
    • >TC001142 RB
      MSERLILSK NQAQIDGIL DGSQKLLQI LTAESRPTS 
      EFNEFITPL PNMVVGSDG RFAYTNHLF NPYEREHWW 
      IPMDHCYAR PWNWRPEST FLRPTKTLF MPKVPPGRK 
      KAINPLAPI QECDDVIDV VTDSEEPTP IYDKAKAKH 
      LMEECEKHA AMARVDEGN EDWEESVSR LNWNTTQNR 
      LFNGVVNIL NADHLARLA HSDSSNEAI ARRVTIDKS 
      VERMRRLMA TVSWDPKYT QWLHQILID HLSTSYLAA 
      YLDILQTLR SKLPTFVDK MMGAANSSK LSILSYETL 
      FPLLKRPWD PVASSLMQD KPKKLPGNP VIVVVPSTP 
      ISSKRMLKW INLLSNLAT VITVPANYG GGLHKTTMM 
      NCVDQMFVI TRNKIQDIR LDYPGRSIV LAGFGFGAT 
      LALQIAQVE QVLCVISIG FSLLTADGK RGEVDDNLL 
      ELQCPILFV IGQCSNTSL QEDMEDLRE RMRVETGLI 
      VVGNADDNL RVSKKKKKA EGITQSIVD RCVADEIGE 
      FISGVILSP YPPQLRQSP TNLTTEGLV SKKLKSERK 
      RYNSNTSSI DSEPPSPTP RISRPGQNS SHLGRPPGS 
      KSKAKMEAK WAAQGIPSS PSNSPPHLS NTPDTSSND 
      SILTDKLSL TNFDPSPVV KKLKTLKPT VVSPEKTTT 
      NQHTSTSNQ NTAKAMGLQ NRLSGLLQG DVSTLIQPS 
      MVKSNSSGI KENVKITGG LNPKFFSNN GKMVDMSKV 
      SVINPKSNN ANSLVLLPD GKIKSYAPS VKVPGGRYI 
      TSKRQLLGN KPPKTVKKV SYISQPMQT NLSPPTNLT 
      TQDIMNLPI IFADDNQIL DNTLTQPEM KPVPSSPNS 
      KLSANTGSK FMLINNKPS NFIISPNPK PSVSQPAKA 
      TPKYTKIIL SSKQTATSN VVKKITNLP SEISVRKVT 
      PSETATISQ SEELDLENE IAASTLYKR KVESTDAEC 
      LEIVSNKPE EEKLEGESV KRTAEEANI DNDESTPCK 
      VSKVETA
    CDS sequence
    • >TC001142 RA
      atggttgtg ggcagcgac ggccgtttc gcttacaca 
      aaccattta tttaaccct tatgaacgg gaacattgg 
      tggatacca atggatcat tgctacgcc agaccgtgg 
      aattggcgc cctgagagc acattcttg cgccccacg 
      aagacgctt tttatgccc aaagtgccc ccagggaga 
      aaaaaagcc attaatccc ctggccccc atacaggaa 
      tgtgacgat gttatagac gttgttaca gatagcgag 
      gaaccaaca ccgatctat gataaagcg aaagctaaa 
      catttgatg gaagaatgc gaaaaacac gccgctatg 
      gccagagtt gatgagggt aatgaggac tgggaagaa 
      agcgtttca agactgaac tggaacacc acacaaaat 
      aggctattt aacggagta gttaatatt ttaaacgct 
      gatcatttg gcacgtttg gcacactct gactcaagc 
      aatgaggca atagccaga cgagtcaca atcgacaaa 
      tctgtcgaa agaatgcgg cgcttaatg gccaccgtt 
      tcatgggac cctaaatat acacagtgg ttgcatcaa 
      attttaatt gaccatctt agcacctca tacctggcc 
      gcgtaccta gatatactt caaaccctt aggtccaag 
      cttcctaca tttgtagat aaaatgatg ggcgctgca 
      aattccagt aaattaagt attttaagt tatgaaaca 
      cttttccct ttgttgaaa cgaccgtgg gaccctgta 
      gcctcgagt ttaatgcag gacaagcca aaaaaatta 
      ccggggaat cccgtgatt gtcgtggtg ccatccacg 
      ccgatttcg tcgaaacgc atgctaaag tggatcaat 
      ttgttgtcc aatctggcc acggtcatc acagtgccc 
      gcaaattac ggcggcggg ctgcataag acgacgatg 
      atgaattgt gtggatcaa atgtttgtt ataacccgg 
      aacaaaatc caggatatc cgtctggat tatcctgga 
      agaagcatc gttttggcc ggtttcggg tttggggca 
      actcttgcg ttacaaata gctcaagtc gagcaagtg 
      ttatgcgtt ataagtatc ggtttttcg cttctaaca 
      gctgacggt aaaagaggc gaagttgat gataatttg 
      ttagaatta cagtgccct attcttttt gttattgga 
      cagtgttca aacacttcg ttgcaggaa gatatggaa 
      gatttgcgg gagagaatg cgggtggaa actggttta 
      attgttgtt gggaatgca gacgataat ttacgcgtg 
      agtaaaaag aagaaaaag gctgagggc ataacccag 
      agcattgtt gatcgatgt gttgctgac gaaattggg 
      gagtttata agtggggta atcctatct ccatatccg 
      ccccaattg agacaatcc ccgactaac ttaactacc 
      gaagggctc gtttctaaa aaattgaaa tcggagcgg 
      aagaggtac aattctaat acaagttcc atagacagt 
      gagccccca tctccaaca ccgaggata agtagacca 
      ggtcaaaac agttctcac ttggggcgc ccgcctggg 
      tccaaatcc aaggccaaa atggaggcc aaatgggca 
      gcgcaaggt atcccgtcc tctcctagt aatagccct 
      ccacaccta tccaacaca cctgacaca tcctccaat 
      gacagtatc ctcacagac aaactatct ctaacaaat 
      ttcgatcca tctcccgtt gttaaaaaa ctcaaaaca 
      ttgaaacca accgtggtt tcgccggaa aaaactaca 
      actaatcaa cacacttca acatcaaac cagaacact 
      gcgaaagcc atgggactg caaaatcga ttgtctggt 
      ttgttacaa ggcgacgtt tcaacgtta atccagcca 
      tcgatggtc aagtcaaat tcgtcgggg atcaaagaa 
      aatgtgaaa ataaccggg ggtttgaac ccgaaattt 
      ttctcaaat aacggtaag atggtagat atgtccaaa 
      gtatcagtg attaaccca aaatcaaat aatgcgaat 
      tcgttagtt ttgttaccc gatggtaaa ataaagagt 
      tacgcgccc agtgtcaaa gtaccagga ggaagatac 
      attacttca aagcgccag ttgttgggt aacaagcca 
      ccaaagacg gttaaaaaa gtgtcatac atctcacaa 
      cccatgcag actaaccta tcccctccc acaaacctg 
      acgactcaa gacatcatg aatttgccg ataattttc 
      gccgatgat aatcaaatt ctagacaac actctcacc 
      caacctgaa atgaaacct gtcccatcg tcaccaaac 
      tcaaaactg tctgccaac actggtagt aaatttatg 
      cttataaac aacaaaccc agcaatttt atcatatca 
      ccgaaccct aaacccagc gtttcacaa cccgcgaaa 
      gctacacca aagtacacg aaaatcatc ctttcgtca 
      aaacaaacc gccacttca aacgtggtg aaaaaaatt 
      acaaacttg ccgtcggaa atatcggtt cggaaggta 
      actccgagc gaaacagcc acaattagc caaagcgaa 
      gaactggat ttggaaaat gagatcgcg gctagcact 
      ttgtacaaa aggaaggtc gaaagtacc gacgccgag 
      tgtttggag attgttagt aacaaacca gaggaggaa 
      aaactcgaa ggggaatcg gtcaagaga acagccgag 
      gaagccaac attgataat gatgaaagc acgccttgt 
      aaagtttcg aaagttgaa actgcttga
    • >TC001142 RB
      atgtctgaa aggttaatt ctgagtaaa aatcaggca 
      caaatcgac ggtatatta gacggttct cagaagctg 
      ttgcaaata ttaacggca gaaagtcgc ccaactagt 
      gaattcaac gagtttata acacctctt ccaaacatg 
      gttgtgggc agcgacggc cgtttcgct tacacaaac 
      catttattt aacccttat gaacgggaa cattggtgg 
      ataccaatg gatcattgc tacgccaga ccgtggaat 
      tggcgccct gagagcaca ttcttgcgc cccacgaag 
      acgcttttt atgcccaaa gtgccccca gggagaaaa 
      aaagccatt aatcccctg gcccccata caggaatgt 
      gacgatgtt atagacgtt gttacagat agcgaggaa 
      ccaacaccg atctatgat aaagcgaaa gctaaacat 
      ttgatggaa gaatgcgaa aaacacgcc gctatggcc 
      agagttgat gagggtaat gaggactgg gaagaaagc 
      gtttcaaga ctgaactgg aacaccaca caaaatagg 
      ctatttaac ggagtagtt aatatttta aacgctgat 
      catttggca cgtttggca cactctgac tcaagcaat 
      gaggcaata gccagacga gtcacaatc gacaaatct 
      gtcgaaaga atgcggcgc ttaatggcc accgtttca 
      tgggaccct aaatataca cagtggttg catcaaatt 
      ttaattgac catcttagc acctcatac ctggccgcg 
      tacctagat atacttcaa acccttagg tccaagctt 
      cctacattt gtagataaa atgatgggc gctgcaaat 
      tccagtaaa ttaagtatt ttaagttat gaaacactt 
      ttccctttg ttgaaacga ccgtgggac cctgtagcc 
      tcgagttta atgcaggac aagccaaaa aaattaccg 
      gggaatccc gtgattgtc gtggtgcca tccacgccg 
      atttcgtcg aaacgcatg ctaaagtgg atcaatttg 
      ttgtccaat ctggccacg gtcatcaca gtgcccgca 
      aattacggc ggcgggctg cataagacg acgatgatg 
      aattgtgtg gatcaaatg tttgttata acccggaac 
      aaaatccag gatatccgt ctggattat cctggaaga 
      agcatcgtt ttggccggt ttcgggttt ggggcaact 
      cttgcgtta caaatagct caagtcgag caagtgtta 
      tgcgttata agtatcggt ttttcgctt ctaacagct 
      gacggtaaa agaggcgaa gttgatgat aatttgtta 
      gaattacag tgccctatt ctttttgtt attggacag 
      tgttcaaac acttcgttg caggaagat atggaagat 
      ttgcgggag agaatgcgg gtggaaact ggtttaatt 
      gttgttggg aatgcagac gataattta cgcgtgagt 
      aaaaagaag aaaaaggct gagggcata acccagagc 
      attgttgat cgatgtgtt gctgacgaa attggggag 
      tttataagt ggggtaatc ctatctcca tatccgccc 
      caattgaga caatccccg actaactta actaccgaa 
      gggctcgtt tctaaaaaa ttgaaatcg gagcggaag 
      aggtacaat tctaataca agttccata gacagtgag 
      cccccatct ccaacaccg aggataagt agaccaggt 
      caaaacagt tctcacttg gggcgcccg cctgggtcc 
      aaatccaag gccaaaatg gaggccaaa tgggcagcg 
      caaggtatc ccgtcctct cctagtaat agccctcca 
      cacctatcc aacacacct gacacatcc tccaatgac 
      agtatcctc acagacaaa ctatctcta acaaatttc 
      gatccatct cccgttgtt aaaaaactc aaaacattg 
      aaaccaacc gtggtttcg ccggaaaaa actacaact 
      aatcaacac acttcaaca tcaaaccag aacactgcg 
      aaagccatg ggactgcaa aatcgattg tctggtttg 
      ttacaaggc gacgtttca acgttaatc cagccatcg 
      atggtcaag tcaaattcg tcggggatc aaagaaaat 
      gtgaaaata accgggggt ttgaacccg aaatttttc 
      tcaaataac ggtaagatg gtagatatg tccaaagta 
      tcagtgatt aacccaaaa tcaaataat gcgaattcg 
      ttagttttg ttacccgat ggtaaaata aagagttac 
      gcgcccagt gtcaaagta ccaggagga agatacatt 
      acttcaaag cgccagttg ttgggtaac aagccacca 
      aagacggtt aaaaaagtg tcatacatc tcacaaccc 
      atgcagact aacctatcc cctcccaca aacctgacg 
      actcaagac atcatgaat ttgccgata attttcgcc 
      gatgataat caaattcta gacaacact ctcacccaa 
      cctgaaatg aaacctgtc ccatcgtca ccaaactca 
      aaactgtct gccaacact ggtagtaaa tttatgctt 
      ataaacaac aaacccagc aattttatc atatcaccg 
      aaccctaaa cccagcgtt tcacaaccc gcgaaagct 
      acaccaaag tacacgaaa atcatcctt tcgtcaaaa 
      caaaccgcc acttcaaac gtggtgaaa aaaattaca 
      aacttgccg tcggaaata tcggttcgg aaggtaact 
      ccgagcgaa acagccaca attagccaa agcgaagaa 
      ctggatttg gaaaatgag atcgcggct agcactttg 
      tacaaaagg aaggtcgaa agtaccgac gccgagtgt 
      ttggagatt gttagtaac aaaccagag gaggaaaaa 
      ctcgaaggg gaatcggtc aagagaaca gccgaggaa 
      gccaacatt gataatgat gaaagcacg ccttgtaaa 
      gtttcgaaa gttgaaact gcttga
    mRNA sequence
    • >TC001142 RA
      tgtttcagc gaaatgtca gaaacaaat gaaacaata 
      gaaagtgat ttgatacca aacaaacat tgaaaaagt 
      gtttttctc caaaaaacc tacactaat tagcttttt 
      tactagtga ggcatgtct gaaaggtta attctgagt 
      aaaaatcag gcacaaatc gacggtata ttagacggt 
      tctcagaag ctggtacac attgaggtt atgttcaat 
      ccccaaaat tgtgtttaa tttgattta gttgcaaat 
      attaacggc agaaagtcg cccaactag tgaattcaa 
      cgagtttat aacacctct tccaaacat ggttgtggg 
      cagcgacgg ccgtttcgc ttacacaaa ccatttatt 
      taaccctta tgaacggga acattggtg gataccaat 
      ggatcattg ctacgccag accgtggaa ttggcgccc 
      tgagagcac attcttgcg ccccacgaa gacgctttt 
      tatgcccaa agtgccccc agggagaaa aaaagccat 
      taatcccct ggcccccat acaggaatg tgacgatgt 
      tatagacgt tgttacaga tagcgagga accaacacc 
      gatctatga taaagcgaa agctaaaca tttgatgga 
      agaatgcga aaaacacgc cgctatggc cagagttga 
      tgagggtaa tgaggactg ggaagaaag cgtttcaag 
      actgaactg gaacaccac acaaaatag gctatttaa 
      cggagtagt taatatttt aaacgctga tcatttggc 
      acgtttggc acactctga ctcaagcaa tgaggcaat 
      agccagacg agtcacaat cgacaaatc tgtcgaaag 
      aatgcggcg cttaatggc caccgtttc atgggaccc 
      taaatatac acagtggtt gcatcaaat tttaattga 
      ccatcttag cacctcata cctggccgc gtacctaga 
      tatacttca aacccttag gtccaagct tcctacatt 
      tgtagataa aatgatggg cgctgcaaa ttccagtaa 
      attaagtat tttaagtta tgaaacact tttcccttt 
      gttgaaacg accgtggga ccctgtagc ctcgagttt 
      aatgcagga caagccaaa aaaattacc ggggaatcc 
      cgtgattgt cgtggtgcc atccacgcc gatttcgtc 
      gaaacgcat gctaaagtg gatcaattt gttgtccaa 
      tctggccac ggtcatcac agtgcccgc aaattacgg 
      cggcgggct gcataagac gacgatgat gaattgtgt 
      ggatcaaat gtttgttat aacccggaa caaaatcca 
      ggatatccg tctggatta tcctggaag aagcatcgt 
      tttggccgg tttcgggtt tggggcaac tcttgcgtt 
      acaaatagc tcaagtcga gcaagtgtt atgcgttat 
      aagtatcgg tttttcgct tctaacagc tgacggtaa 
      aagaggcga agttgatga taatttgtt agaattaca 
      gtgccctat tctttttgt tattggaca gtgttcaaa 
      cacttcgtt gcaggaaga tatggaaga tttgcggga 
      gagaatgcg ggtggaaac tggtttaat tgttgttgg 
      gaatgcaga cgataattt acgcgtgag taaaaagaa 
      gaaaaaggc tgagggcat aacccagag cattgttga 
      tcgatgtgt tgctgacga aattgggga gtttataag 
      tggggtaat cctatctcc atatccgcc ccaattgag 
      acaatcccc gactaactt aactaccga agggctcgt 
      ttctaaaaa attgaaatc ggagcggaa gaggtacaa 
      ttctaatac aagttccat agacagtga gcccccatc 
      tccaacacc gaggataag tagaccagg tcaaaacag 
      ttctcactt ggggcgccc gcctgggtc caaatccaa 
      ggccaaaat ggaggccaa atgggcagc gcaaggtat 
      cccgtcctc tcctagtaa tagccctcc acacctatc 
      caacacacc tgacacatc ctccaatga cagtatcct 
      cacagacaa actatctct aacaaattt cgatccatc 
      tcccgttgt taaaaaact caaaacatt gaaaccaac 
      cgtggtttc gccggaaaa aactacaac taatcaaca 
      cacttcaac atcaaacca gaacactgc gaaagccat 
      gggactgca aaatcgatt gtctggttt gttacaagg 
      cgacgtttc aacgttaat ccagccatc gatggtcaa 
      gtcaaattc gtcggggat caaagaaaa tgtgaaaat 
      aaccggggg tttgaaccc gaaattttt ctcaaataa 
      cggtaagat ggtagatat gtccaaagt atcagtgat 
      taacccaaa atcaaataa tgcgaattc gttagtttt 
      gttacccga tggtaaaat aaagagtta cgcgcccag 
      tgtcaaagt accaggagg aagatacat tacttcaaa 
      gcgccagtt gttgggtaa caagccacc aaagacggt 
      taaaaaagt gtcatacat ctcacaacc catgcagac 
      taacctatc ccctcccac aaacctgac gactcaaga 
      catcatgaa tttgccgat aattttcgc cgatgataa 
      tcaaattct agacaacac tctcaccca acctgaaat 
      gaaacctgt cccatcgtc accaaactc aaaactgtc 
      tgccaacac tggtagtaa atttatgct tataaacaa 
      caaacccag caattttat catatcacc gaaccctaa 
      acccagcgt ttcacaacc cgcgaaagc tacaccaaa 
      gtacacgaa aatcatcct ttcgtcaaa acaaaccgc 
      cacttcaaa cgtggtgaa aaaaattac aaacttgcc 
      gtcggaaat atcggttcg gaaggtaac tccgagcga 
      aacagccac aattagcca aagcgaaga actggattt 
      ggaaaatga gatcgcggc tagcacttt gtacaaaag 
      gaaggtcga aagtaccga cgccgagtg tttggagat 
      tgttagtaa caaaccaga ggaggaaaa actcgaagg 
      ggaatcggt caagagaac agccgagga agccaacat 
      tgataatga tgaaagcac gccttgtaa agtttcgaa 
      agttgaaac tgcttgagt tatttaacc aaatatatt 
      tgttacacg tta
    • >TC001142 RB
      tgtttcagc gaaatgtca gaaacaaat gaaacaata 
      gaaagtgat ttgatacca aacaaacat tgaaaaagt 
      gtttttctc caaaaaacc tacactaat tagcttttt 
      tactagtga ggcatgtct gaaaggtta attctgagt 
      aaaaatcag gcacaaatc gacggtata ttagacggt 
      tctcagaag ctgttgcaa atattaacg gcagaaagt 
      cgcccaact agtgaattc aacgagttt ataacacct 
      cttccaaac atggttgtg ggcagcgac ggccgtttc 
      gcttacaca aaccattta tttaaccct tatgaacgg 
      gaacattgg tggatacca atggatcat tgctacgcc 
      agaccgtgg aattggcgc cctgagagc acattcttg 
      cgccccacg aagacgctt tttatgccc aaagtgccc 
      ccagggaga aaaaaagcc attaatccc ctggccccc 
      atacaggaa tgtgacgat gttatagac gttgttaca 
      gatagcgag gaaccaaca ccgatctat gataaagcg 
      aaagctaaa catttgatg gaagaatgc gaaaaacac 
      gccgctatg gccagagtt gatgagggt aatgaggac 
      tgggaagaa agcgtttca agactgaac tggaacacc 
      acacaaaat aggctattt aacggagta gttaatatt 
      ttaaacgct gatcatttg gcacgtttg gcacactct 
      gactcaagc aatgaggca atagccaga cgagtcaca 
      atcgacaaa tctgtcgaa agaatgcgg cgcttaatg 
      gccaccgtt tcatgggac cctaaatat acacagtgg 
      ttgcatcaa attttaatt gaccatctt agcacctca 
      tacctggcc gcgtaccta gatatactt caaaccctt 
      aggtccaag cttcctaca tttgtagat aaaatgatg 
      ggcgctgca aattccagt aaattaagt attttaagt 
      tatgaaaca cttttccct ttgttgaaa cgaccgtgg 
      gaccctgta gcctcgagt ttaatgcag gacaagcca 
      aaaaaatta ccggggaat cccgtgatt gtcgtggtg 
      ccatccacg ccgatttcg tcgaaacgc atgctaaag 
      tggatcaat ttgttgtcc aatctggcc acggtcatc 
      acagtgccc gcaaattac ggcggcggg ctgcataag 
      acgacgatg atgaattgt gtggatcaa atgtttgtt 
      ataacccgg aacaaaatc caggatatc cgtctggat 
      tatcctgga agaagcatc gttttggcc ggtttcggg 
      tttggggca actcttgcg ttacaaata gctcaagtc 
      gagcaagtg ttatgcgtt ataagtatc ggtttttcg 
      cttctaaca gctgacggt aaaagaggc gaagttgat 
      gataatttg ttagaatta cagtgccct attcttttt 
      gttattgga cagtgttca aacacttcg ttgcaggaa 
      gatatggaa gatttgcgg gagagaatg cgggtggaa 
      actggttta attgttgtt gggaatgca gacgataat 
      ttacgcgtg agtaaaaag aagaaaaag gctgagggc 
      ataacccag agcattgtt gatcgatgt gttgctgac 
      gaaattggg gagtttata agtggggta atcctatct 
      ccatatccg ccccaattg agacaatcc ccgactaac 
      ttaactacc gaagggctc gtttctaaa aaattgaaa 
      tcggagcgg aagaggtac aattctaat acaagttcc 
      atagacagt gagccccca tctccaaca ccgaggata 
      agtagacca ggtcaaaac agttctcac ttggggcgc 
      ccgcctggg tccaaatcc aaggccaaa atggaggcc 
      aaatgggca gcgcaaggt atcccgtcc tctcctagt 
      aatagccct ccacaccta tccaacaca cctgacaca 
      tcctccaat gacagtatc ctcacagac aaactatct 
      ctaacaaat ttcgatcca tctcccgtt gttaaaaaa 
      ctcaaaaca ttgaaacca accgtggtt tcgccggaa 
      aaaactaca actaatcaa cacacttca acatcaaac 
      cagaacact gcgaaagcc atgggactg caaaatcga 
      ttgtctggt ttgttacaa ggcgacgtt tcaacgtta 
      atccagcca tcgatggtc aagtcaaat tcgtcgggg 
      atcaaagaa aatgtgaaa ataaccggg ggtttgaac 
      ccgaaattt ttctcaaat aacggtaag atggtagat 
      atgtccaaa gtatcagtg attaaccca aaatcaaat 
      aatgcgaat tcgttagtt ttgttaccc gatggtaaa 
      ataaagagt tacgcgccc agtgtcaaa gtaccagga 
      ggaagatac attacttca aagcgccag ttgttgggt 
      aacaagcca ccaaagacg gttaaaaaa gtgtcatac 
      atctcacaa cccatgcag actaaccta tcccctccc 
      acaaacctg acgactcaa gacatcatg aatttgccg 
      ataattttc gccgatgat aatcaaatt ctagacaac 
      actctcacc caacctgaa atgaaacct gtcccatcg 
      tcaccaaac tcaaaactg tctgccaac actggtagt 
      aaatttatg cttataaac aacaaaccc agcaatttt 
      atcatatca ccgaaccct aaacccagc gtttcacaa 
      cccgcgaaa gctacacca aagtacacg aaaatcatc 
      ctttcgtca aaacaaacc gccacttca aacgtggtg 
      aaaaaaatt acaaacttg ccgtcggaa atatcggtt 
      cggaaggta actccgagc gaaacagcc acaattagc 
      caaagcgaa gaactggat ttggaaaat gagatcgcg 
      gctagcact ttgtacaaa aggaaggtc gaaagtacc 
      gacgccgag tgtttggag attgttagt aacaaacca 
      gaggaggaa aaactcgaa ggggaatcg gtcaagaga 
      acagccgag gaagccaac attgataat gatgaaagc 
      acgccttgt aaagtttcg aaagttgaa actgcttga 
      gttatttaa ccaaatata tttgttaca cgtta