Status
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Gene information
General information
TC032179
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001137,TC001138,TC001136
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11485552..11492285
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032179
    iBeetle screen iB_00217

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032179 RA
      MAPKDNLTA VLYGINDMR LEQRPIPVP KPNQVLLKM 
      EVVGICGSD VHYLVSGRI GPFVVTDPM VIGHEASGT 
      VIQVGRDVK NLKPGDRVA IEPGITCRT CADCKSGNY 
      HLCKDMIFC ATPPVDGNL TRYYVHDAD FCHKLPDNM 
      DLEEGALME PLSVGVHAC RRAGVRIGS VVLVLGAGP 
      IGLVSMLTA KAMGASKVI ITDIVGHRL QKAKELGAD 
      FTLQIGQNT TEEEIVSEI KAKLGEDPN ITLECTGAE 
      QCVRVALQV TKSGGTVIL VGLGKFEMT VPLAGALVR 
      EVNIRGVFR YNNDYPIAI EMVKTGKVN VKPLITHHY 
      KMEDTLKAF HTAKTGEGN PIKDNLTAV LYGIDDMRL 
      EQRPIPVPK DNQVLLKME SVGICGSDV HYLVQGRIG 
      PFIVEKPMI IGHEASGTV VLCGKNVTS LKPGDRVAI 
      EPGVGCRMC SFCKEGNYH LCLDMQFCA TPPVDGNLS 
      RFYVHDADF CFKLPDNVS LDEGALMEP LAVGVHACK 
      RANVRFGDV VLILGAGPI GLVTLLAAK AMGATKALI 
      TDIVDIRLT KAKELGADY TLKIEKNMT EEEIIKKIK 
      ALLGEEPNV SLDCTGAEQ CVRVAVQAT KSGGVVTLI 
      GLGAFEMNL PLTGALIRE VDIRGVFRY NNDYPTAIE 
      MVRSGKANV KSLITHHYK IEDTLKAFH TAKTGEGNP 
      IKARFSCCS KSSQKNNLA AVLHGIGDL RLENRPMPI 
      IKDNQVLLQ METVGICGS DVHYLVEGR IGPFVVKNP 
      MVIGHEASG TVLEIGKKV KTLKPGDRV AIEPGVGCR 
      VCTFCKDGR YHLCPEMAF CATPPIDGN LCRFFAHDA 
      DFCFKLPEH LTLDEGALM EPLSVAVHS CKRANVRLG 
      DVVLVMGAG PIGLTSLLA ARAYGASAV LITDLAEHR 
      LNKARELGA DCVLKVEKN MREEELVKE IKCLLRVDP 
      NITIECTGE ESSIRASLQ VTKTGGVVV LVGLGKFDL 
      NLPIFPLFR EVDVRGIFR YNNDYPQAI EMVQSGKAN 
      VKPLITHHF AMEDTVKAF ETARTGAGN PIKILIHAN 
      PDWKICE
    CDS sequence
    • >TC032179 RA
      atggcacca aaggataac ttaacagcc gttttgtac 
      ggcatcaac gacatgcga cttgaacaa agaccaatc 
      cctgtcccc aaaccaaat caagtactt cttaaaatg 
      gaagtcgtg ggtatttgt ggctccgat gtccattac 
      ttggtcagt ggcagaatt ggccctttt gtggttaca 
      gatcccatg gttatagga cacgaagcc tccggaacg 
      gtaatacaa gtcggacgc gacgtcaaa aatcttaaa 
      ccaggtgat cgtgtggct atcgagcct ggaataact 
      tgccgcact tgtgctgac tgcaaaagc ggcaattac 
      cacctgtgc aaggacatg attttttgt gcaactccg 
      ccagttgat gggaatttg actagatat tacgtccac 
      gatgccgat ttttgccac aagttgccc gataacatg 
      gacttggaa gagggtgcc cttatggag cccctctcc 
      gtgggtgtc cacgcgtgc aggagggcc ggagtgcgc 
      ataggttcc gtggttttg gttctaggt gctggcccg 
      attggtcta gtttcaatg cttacagcc aaggcaatg 
      ggtgcttcc aaagtcata attacagat attgtgggc 
      caccgattg caaaaagct aaagagtta ggtgctgat 
      ttcacccta caaattggt caaaacacg actgaagaa 
      gaaatcgtg tcagagatt aaagcaaaa ttgggggaa 
      gacccgaat atcactttg gaatgcacg ggggccgaa 
      caatgcgtc cgagttgct ttacaagtc acaaaatcc 
      ggaggtact gttattttg gtaggttta gggaaattt 
      gaaatgacc gtgcctctg gcgggtgct ttggtacgc 
      gaagtcaac atacgtgga gtgttccga tacaataat 
      gattatccg atcgcaatc gaaatggtc aaaacaggc 
      aaagttaat gtgaaacca ctaataacg caccactat 
      aaaatggaa gatacgctg aaagctttt cacactgct 
      aagacaggc gaaggaaat ccgatcaag gataattta 
      acagccgtc ctttacggc atcgacgac atgcgtttg 
      gaacaacga ccgattccc gtccccaaa gacaaccaa 
      gtcctgctt aaaatggaa tcagtcgga atttgcggc 
      tcagacgtg cattattta gtccagggc cggatcggg 
      cctttcatc gtcgaaaaa cccatgata atcggtcac 
      gaggcgtcc ggaacggtc gttttgtgc ggcaaaaat 
      gtcacctcc ttgaaaccc ggcgaccgc gtggccatt 
      gagcccggc gttggctgc aggatgtgc tctttctgc 
      aaagagggc aactaccac ctttgcctc gacatgcaa 
      ttttgcgcc acgccgcca gtggacggc aacctctcc 
      agattctac gtccacgac gccgatttc tgcttcaaa 
      cttccggac aatgtgagc cttgacgaa ggcgccctg 
      atggagccg ctggcggtg ggcgtgcac gcgtgtaag 
      cgcgcaaat gtgcgcttc ggcgacgtg gttttgatt 
      ctgggggct gggcccatt gggcttgtt acgcttttg 
      gctgctaag gccatgggg gccaccaag gccctaatc 
      actgacatt gttgacata cgattaacc aaagccaaa 
      gaactgggg gctgactat actttgaaa attgaaaag 
      aacatgacc gaggaagag ataataaaa aaaattaag 
      gccctgtta ggggaagaa cctaacgtt agtcttgac 
      tgcactggt gccgaacaa tgtgttagg gttgctgtc 
      caggcaacc aaaagtgga ggtgtggtc acgttgatt 
      ggtttagga gctttcgaa atgaatttg ccgttaacg 
      ggcgctctt attcgagaa gttgacatt cgaggtgtt 
      ttccgctat aataatgat tatccgact gctattgaa 
      atggtgaga agtggaaaa gcaaatgtt aaatctttg 
      attacgcat cactacaag attgaagat actttgaaa 
      gcgttccat acggcgaaa acaggcgaa ggaaatccg 
      attaaagcc aggtttagt tgctgtagc aaatccagt 
      cagaaaaat aacttagct gcggtcctg cacggcatt 
      ggagattta cgcttggaa aatagaccc atgcccatc 
      attaaagac aatcaggtg ctccttcaa atggaaaca 
      gtgggcatt tgtggctca gacgtccat taccttgtg 
      gaaggccga attggtccg tttgtggta aaaaaccca 
      atggtcatt gggcacgag gcttccgga accgtcctc 
      gaaatcgga aaaaaagtc aaaactctt aaaccaggt 
      gaccgagtg gcaatcgaa ccgggggtt ggttgtcgg 
      gtttgcaca ttttgcaaa gacggtaga tatcacctg 
      tgtcccgag atggcattt tgtgcaact cctcccatc 
      gatggaaac ttgtgcaga ttttttgcc cacgatgca 
      gatttttgt ttcaaatta cccgagcac ttgactctc 
      gatgagggt gctttaatg gaaccctta tctgtcgct 
      gtgcactct tgcaagcgg gcaaatgtt cgtttaggg 
      gacgtggtt ctagtgatg ggagctggt cccattgga 
      ctcacttcc ctgcttgca gcaagagct tatggtgcc 
      tctgctgtt cttataaca gatttggca gaacatcgc 
      ttgaataaa gcgagagaa cttggagct gattgcgtc 
      cttaaagtt gagaaaaat atgcgagaa gaagagctt 
      gtgaaggag attaaatgc ttgctgagg gtggaccca 
      aatattaca atagaatgt acaggggag gaatctagc 
      attagagct tccctccag gttacaaaa actggaggt 
      gttgtggtt ttagtcggt ttgggaaaa tttgaccta 
      aatctacca atttttccc ttatttcgg gaggttgat 
      gttagaggg atatttcgc tataataat gactatccg 
      caagctata gaaatggtg cagagtgga aaagcgaat 
      gtgaagccc ctaattaca catcatttt gcaatggaa 
      gacacggtt aaagcgttc gagaccgca agaactggg 
      gcagggaat ccaattaaa atcctgatt catgccaat 
      ccagactgg aagatttgt gaataa
    mRNA sequence
    • >TC032179 RA
      cacattcgg aaaatggca ccaaaggat aacttaaca 
      gccgttttg tacggcatc aacgacatg cgacttgaa 
      caaagacca atccctgtc cccaaacca aatcaagta 
      cttcttaaa atggaagtc gtgggtatt tgtggctcc 
      gatgtccat tacttggtc agtggcaga attggccct 
      tttgtggtt acagatccc atggttata ggacacgaa 
      gcctccgga acggtaata caagtcgga cgcgacgtc 
      aaaaatctt aaaccaggt gatcgtgtg gctatcgag 
      cctggaata acttgccgc acttgtgct gactgcaaa 
      agcggcaat taccacctg tgcaaggac atgattttt 
      tgtgcaact ccgccagtt gatgggaat ttgactaga 
      tattacgtc cacgatgcc gatttttgc cacaagttg 
      cccgataac atggacttg gaagagggt gcccttatg 
      gagcccctc tccgtgggt gtccacgcg tgcaggagg 
      gccggagtg cgcataggt tccgtggtt ttggttcta 
      ggtgctggc ccgattggt ctagtttca atgcttaca 
      gccaaggca atgggtgct tccaaagtc ataattaca 
      gatattgtg ggccaccga ttgcaaaaa gctaaagag 
      ttaggtgct gatttcacc ctacaaatt ggtcaaaac 
      acgactgaa gaagaaatc gtgtcagag attaaagca 
      aaattgggg gaagacccg aatatcact ttggaatgc 
      acgggggcc gaacaatgc gtccgagtt gctttacaa 
      gtcacaaaa tccggaggt actgttatt ttggtaggt 
      ttagggaaa tttgaaatg accgtgcct ctggcgggt 
      gctttggta cgcgaagtc aacatacgt ggagtgttc 
      cgatacaat aatgattat ccgatcgca atcgaaatg 
      gtcaaaaca ggcaaagtt aatgtgaaa ccactaata 
      acgcaccac tataaaatg gaagatacg ctgaaagct 
      tttcacact gctaagaca ggcgaagga aatccgatc 
      aaggataat ttaacagcc gtcctttac ggcatcgac 
      gacatgcgt ttggaacaa cgaccgatt cccgtcccc 
      aaagacaac caagtcctg cttaaaatg gaatcagtc 
      ggaatttgc ggctcagac gtgcattat ttagtccag 
      ggccggatc gggcctttc atcgtcgaa aaacccatg 
      ataatcggt cacgaggcg tccggaacg gtcgttttg 
      tgcggcaaa aatgtcacc tccttgaaa cccggcgac 
      cgcgtggcc attgagccc ggcgttggc tgcaggatg 
      tgctctttc tgcaaagag ggcaactac cacctttgc 
      ctcgacatg caattttgc gccacgccg ccagtggac 
      ggcaacctc tccagattc tacgtccac gacgccgat 
      ttctgcttc aaacttccg gacaatgtg agccttgac 
      gaaggcgcc ctgatggag ccgctggcg gtgggcgtg 
      cacgcgtgt aagcgcgca aatgtgcgc ttcggcgac 
      gtggttttg attctgggg gctgggccc attgggctt 
      gttacgctt ttggctgct aaggccatg ggggccacc 
      aaggcccta atcactgac attgttgac atacgatta 
      accaaagcc aaagaactg ggggctgac tatactttg 
      aaaattgaa aagaacatg accgaggaa gagataata 
      aaaaaaatt aaggccctg ttaggggaa gaacctaac 
      gttagtctt gactgcact ggtgccgaa caatgtgtt 
      agggttgct gtccaggca accaaaagt ggaggtgtg 
      gtcacgttg attggttta ggagctttc gaaatgaat 
      ttgccgtta acgggcgct cttattcga gaagttgac 
      attcgaggt gttttccgc tataataat gattatccg 
      actgctatt gaaatggtg agaagtgga aaagcaaat 
      gttaaatct ttgattacg catcactac aagattgaa 
      gatactttg aaagcgttc catacggcg aaaacaggc 
      gaaggaaat ccgattaaa gccaggttt agttgctgt 
      agcaaatcc agtcagaaa aataactta gctgcggtc 
      ctgcacggc attggagat ttacgcttg gaaaataga 
      cccatgccc atcattaaa gacaatcag gtgctcctt 
      caaatggaa acagtgggc atttgtggc tcagacgtc 
      cattacctt gtggaaggc cgaattggt ccgtttgtg 
      gtaaaaaac ccaatggtc attgggcac gaggcttcc 
      ggaaccgtc ctcgaaatc ggaaaaaaa gtcaaaact 
      cttaaacca ggtgaccga gtggcaatc gaaccgggg 
      gttggttgt cgggtttgc acattttgc aaagacggt 
      agatatcac ctgtgtccc gagatggca ttttgtgca 
      actcctccc atcgatgga aacttgtgc agatttttt 
      gcccacgat gcagatttt tgtttcaaa ttacccgag 
      cacttgact ctcgatgag ggtgcttta atggaaccc 
      ttatctgtc gctgtgcac tcttgcaag cgggcaaat 
      gttcgttta ggggacgtg gttctagtg atgggagct 
      ggtcccatt ggactcact tccctgctt gcagcaaga 
      gcttatggt gcctctgct gttcttata acagatttg 
      gcagaacat cgcttgaat aaagcgaga gaacttgga 
      gctgattgc gtccttaaa gttgagaaa aatatgcga 
      gaagaagag cttgtgaag gagattaaa tgcttgctg 
      agggtggac ccaaatatt acaatagaa tgtacaggg 
      gaggaatct agcattaga gcttccctc caggttaca 
      aaaactgga ggtgttgtg gttttagtc ggtttggga 
      aaatttgac ctaaatcta ccaattttt cccttattt 
      cgggaggtt gatgttaga gggatattt cgctataat 
      aatgactat ccgcaagct atagaaatg gtgcagagt 
      ggaaaagcg aatgtgaag cccctaatt acacatcat 
      tttgcaatg gaagacacg gttaaagcg ttcgagacc 
      gcaagaact ggggcaggg aatccaatt aaaatcctg 
      attcatgcc aatccagac tggaagatt tgtgaataa 
      acaaatatg tatttattt tcccgaagt gttttttat 
      taaattaag cgtgcgcgt tcgcgagag agacggcag 
      gattctgtg gagaaacgc cagaattca atttccaat 
      cggatcaat ggccgatga ccgatcaga ggctaaata 
      acatctgat ttggagcac caggagaat ataaggagg 
      tggaggtaa ccgttaagc cgccaatat ttccgatag 
      gttcacatc aatcgattt tatgctcgc ttggcgcgc 
      gataaattg tttattaaa tttggcacg atttcaaat 
      tatttccag aacggacgc tctgtgatg cgagggatt 
      atgttacta aaacttcat tccgctc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY