| General information | |||
| TC032179
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 11485552..11492285 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC032179 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00217 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
- >TC032179 RA
MAPKDNLTA VLYGINDMR LEQRPIPVP KPNQVLLKM EVVGICGSD VHYLVSGRI GPFVVTDPM VIGHEASGT VIQVGRDVK NLKPGDRVA IEPGITCRT CADCKSGNY HLCKDMIFC ATPPVDGNL TRYYVHDAD FCHKLPDNM DLEEGALME PLSVGVHAC RRAGVRIGS VVLVLGAGP IGLVSMLTA KAMGASKVI ITDIVGHRL QKAKELGAD FTLQIGQNT TEEEIVSEI KAKLGEDPN ITLECTGAE QCVRVALQV TKSGGTVIL VGLGKFEMT VPLAGALVR EVNIRGVFR YNNDYPIAI EMVKTGKVN VKPLITHHY KMEDTLKAF HTAKTGEGN PIKDNLTAV LYGIDDMRL EQRPIPVPK DNQVLLKME SVGICGSDV HYLVQGRIG PFIVEKPMI IGHEASGTV VLCGKNVTS LKPGDRVAI EPGVGCRMC SFCKEGNYH LCLDMQFCA TPPVDGNLS RFYVHDADF CFKLPDNVS LDEGALMEP LAVGVHACK RANVRFGDV VLILGAGPI GLVTLLAAK AMGATKALI TDIVDIRLT KAKELGADY TLKIEKNMT EEEIIKKIK ALLGEEPNV SLDCTGAEQ CVRVAVQAT KSGGVVTLI GLGAFEMNL PLTGALIRE VDIRGVFRY NNDYPTAIE MVRSGKANV KSLITHHYK IEDTLKAFH TAKTGEGNP IKARFSCCS KSSQKNNLA AVLHGIGDL RLENRPMPI IKDNQVLLQ METVGICGS DVHYLVEGR IGPFVVKNP MVIGHEASG TVLEIGKKV KTLKPGDRV AIEPGVGCR VCTFCKDGR YHLCPEMAF CATPPIDGN LCRFFAHDA DFCFKLPEH LTLDEGALM EPLSVAVHS CKRANVRLG DVVLVMGAG PIGLTSLLA ARAYGASAV LITDLAEHR LNKARELGA DCVLKVEKN MREEELVKE IKCLLRVDP NITIECTGE ESSIRASLQ VTKTGGVVV LVGLGKFDL NLPIFPLFR EVDVRGIFR YNNDYPQAI EMVQSGKAN VKPLITHHF AMEDTVKAF ETARTGAGN PIKILIHAN PDWKICE
- >TC032179 RA
atggcacca aaggataac ttaacagcc gttttgtac ggcatcaac gacatgcga cttgaacaa agaccaatc cctgtcccc aaaccaaat caagtactt cttaaaatg gaagtcgtg ggtatttgt ggctccgat gtccattac ttggtcagt ggcagaatt ggccctttt gtggttaca gatcccatg gttatagga cacgaagcc tccggaacg gtaatacaa gtcggacgc gacgtcaaa aatcttaaa ccaggtgat cgtgtggct atcgagcct ggaataact tgccgcact tgtgctgac tgcaaaagc ggcaattac cacctgtgc aaggacatg attttttgt gcaactccg ccagttgat gggaatttg actagatat tacgtccac gatgccgat ttttgccac aagttgccc gataacatg gacttggaa gagggtgcc cttatggag cccctctcc gtgggtgtc cacgcgtgc aggagggcc ggagtgcgc ataggttcc gtggttttg gttctaggt gctggcccg attggtcta gtttcaatg cttacagcc aaggcaatg ggtgcttcc aaagtcata attacagat attgtgggc caccgattg caaaaagct aaagagtta ggtgctgat ttcacccta caaattggt caaaacacg actgaagaa gaaatcgtg tcagagatt aaagcaaaa ttgggggaa gacccgaat atcactttg gaatgcacg ggggccgaa caatgcgtc cgagttgct ttacaagtc acaaaatcc ggaggtact gttattttg gtaggttta gggaaattt gaaatgacc gtgcctctg gcgggtgct ttggtacgc gaagtcaac atacgtgga gtgttccga tacaataat gattatccg atcgcaatc gaaatggtc aaaacaggc aaagttaat gtgaaacca ctaataacg caccactat aaaatggaa gatacgctg aaagctttt cacactgct aagacaggc gaaggaaat ccgatcaag gataattta acagccgtc ctttacggc atcgacgac atgcgtttg gaacaacga ccgattccc gtccccaaa gacaaccaa gtcctgctt aaaatggaa tcagtcgga atttgcggc tcagacgtg cattattta gtccagggc cggatcggg cctttcatc gtcgaaaaa cccatgata atcggtcac gaggcgtcc ggaacggtc gttttgtgc ggcaaaaat gtcacctcc ttgaaaccc ggcgaccgc gtggccatt gagcccggc gttggctgc aggatgtgc tctttctgc aaagagggc aactaccac ctttgcctc gacatgcaa ttttgcgcc acgccgcca gtggacggc aacctctcc agattctac gtccacgac gccgatttc tgcttcaaa cttccggac aatgtgagc cttgacgaa ggcgccctg atggagccg ctggcggtg ggcgtgcac gcgtgtaag cgcgcaaat gtgcgcttc ggcgacgtg gttttgatt ctgggggct gggcccatt gggcttgtt acgcttttg gctgctaag gccatgggg gccaccaag gccctaatc actgacatt gttgacata cgattaacc aaagccaaa gaactgggg gctgactat actttgaaa attgaaaag aacatgacc gaggaagag ataataaaa aaaattaag gccctgtta ggggaagaa cctaacgtt agtcttgac tgcactggt gccgaacaa tgtgttagg gttgctgtc caggcaacc aaaagtgga ggtgtggtc acgttgatt ggtttagga gctttcgaa atgaatttg ccgttaacg ggcgctctt attcgagaa gttgacatt cgaggtgtt ttccgctat aataatgat tatccgact gctattgaa atggtgaga agtggaaaa gcaaatgtt aaatctttg attacgcat cactacaag attgaagat actttgaaa gcgttccat acggcgaaa acaggcgaa ggaaatccg attaaagcc aggtttagt tgctgtagc aaatccagt cagaaaaat aacttagct gcggtcctg cacggcatt ggagattta cgcttggaa aatagaccc atgcccatc attaaagac aatcaggtg ctccttcaa atggaaaca gtgggcatt tgtggctca gacgtccat taccttgtg gaaggccga attggtccg tttgtggta aaaaaccca atggtcatt gggcacgag gcttccgga accgtcctc gaaatcgga aaaaaagtc aaaactctt aaaccaggt gaccgagtg gcaatcgaa ccgggggtt ggttgtcgg gtttgcaca ttttgcaaa gacggtaga tatcacctg tgtcccgag atggcattt tgtgcaact cctcccatc gatggaaac ttgtgcaga ttttttgcc cacgatgca gatttttgt ttcaaatta cccgagcac ttgactctc gatgagggt gctttaatg gaaccctta tctgtcgct gtgcactct tgcaagcgg gcaaatgtt cgtttaggg gacgtggtt ctagtgatg ggagctggt cccattgga ctcacttcc ctgcttgca gcaagagct tatggtgcc tctgctgtt cttataaca gatttggca gaacatcgc ttgaataaa gcgagagaa cttggagct gattgcgtc cttaaagtt gagaaaaat atgcgagaa gaagagctt gtgaaggag attaaatgc ttgctgagg gtggaccca aatattaca atagaatgt acaggggag gaatctagc attagagct tccctccag gttacaaaa actggaggt gttgtggtt ttagtcggt ttgggaaaa tttgaccta aatctacca atttttccc ttatttcgg gaggttgat gttagaggg atatttcgc tataataat gactatccg caagctata gaaatggtg cagagtgga aaagcgaat gtgaagccc ctaattaca catcatttt gcaatggaa gacacggtt aaagcgttc gagaccgca agaactggg gcagggaat ccaattaaa atcctgatt catgccaat ccagactgg aagatttgt gaataa
- >TC032179 RA
cacattcgg aaaatggca ccaaaggat aacttaaca gccgttttg tacggcatc aacgacatg cgacttgaa caaagacca atccctgtc cccaaacca aatcaagta cttcttaaa atggaagtc gtgggtatt tgtggctcc gatgtccat tacttggtc agtggcaga attggccct tttgtggtt acagatccc atggttata ggacacgaa gcctccgga acggtaata caagtcgga cgcgacgtc aaaaatctt aaaccaggt gatcgtgtg gctatcgag cctggaata acttgccgc acttgtgct gactgcaaa agcggcaat taccacctg tgcaaggac atgattttt tgtgcaact ccgccagtt gatgggaat ttgactaga tattacgtc cacgatgcc gatttttgc cacaagttg cccgataac atggacttg gaagagggt gcccttatg gagcccctc tccgtgggt gtccacgcg tgcaggagg gccggagtg cgcataggt tccgtggtt ttggttcta ggtgctggc ccgattggt ctagtttca atgcttaca gccaaggca atgggtgct tccaaagtc ataattaca gatattgtg ggccaccga ttgcaaaaa gctaaagag ttaggtgct gatttcacc ctacaaatt ggtcaaaac acgactgaa gaagaaatc gtgtcagag attaaagca aaattgggg gaagacccg aatatcact ttggaatgc acgggggcc gaacaatgc gtccgagtt gctttacaa gtcacaaaa tccggaggt actgttatt ttggtaggt ttagggaaa tttgaaatg accgtgcct ctggcgggt gctttggta cgcgaagtc aacatacgt ggagtgttc cgatacaat aatgattat ccgatcgca atcgaaatg gtcaaaaca ggcaaagtt aatgtgaaa ccactaata acgcaccac tataaaatg gaagatacg ctgaaagct tttcacact gctaagaca ggcgaagga aatccgatc aaggataat ttaacagcc gtcctttac ggcatcgac gacatgcgt ttggaacaa cgaccgatt cccgtcccc aaagacaac caagtcctg cttaaaatg gaatcagtc ggaatttgc ggctcagac gtgcattat ttagtccag ggccggatc gggcctttc atcgtcgaa aaacccatg ataatcggt cacgaggcg tccggaacg gtcgttttg tgcggcaaa aatgtcacc tccttgaaa cccggcgac cgcgtggcc attgagccc ggcgttggc tgcaggatg tgctctttc tgcaaagag ggcaactac cacctttgc ctcgacatg caattttgc gccacgccg ccagtggac ggcaacctc tccagattc tacgtccac gacgccgat ttctgcttc aaacttccg gacaatgtg agccttgac gaaggcgcc ctgatggag ccgctggcg gtgggcgtg cacgcgtgt aagcgcgca aatgtgcgc ttcggcgac gtggttttg attctgggg gctgggccc attgggctt gttacgctt ttggctgct aaggccatg ggggccacc aaggcccta atcactgac attgttgac atacgatta accaaagcc aaagaactg ggggctgac tatactttg aaaattgaa aagaacatg accgaggaa gagataata aaaaaaatt aaggccctg ttaggggaa gaacctaac gttagtctt gactgcact ggtgccgaa caatgtgtt agggttgct gtccaggca accaaaagt ggaggtgtg gtcacgttg attggttta ggagctttc gaaatgaat ttgccgtta acgggcgct cttattcga gaagttgac attcgaggt gttttccgc tataataat gattatccg actgctatt gaaatggtg agaagtgga aaagcaaat gttaaatct ttgattacg catcactac aagattgaa gatactttg aaagcgttc catacggcg aaaacaggc gaaggaaat ccgattaaa gccaggttt agttgctgt agcaaatcc agtcagaaa aataactta gctgcggtc ctgcacggc attggagat ttacgcttg gaaaataga cccatgccc atcattaaa gacaatcag gtgctcctt caaatggaa acagtgggc atttgtggc tcagacgtc cattacctt gtggaaggc cgaattggt ccgtttgtg gtaaaaaac ccaatggtc attgggcac gaggcttcc ggaaccgtc ctcgaaatc ggaaaaaaa gtcaaaact cttaaacca ggtgaccga gtggcaatc gaaccgggg gttggttgt cgggtttgc acattttgc aaagacggt agatatcac ctgtgtccc gagatggca ttttgtgca actcctccc atcgatgga aacttgtgc agatttttt gcccacgat gcagatttt tgtttcaaa ttacccgag cacttgact ctcgatgag ggtgcttta atggaaccc ttatctgtc gctgtgcac tcttgcaag cgggcaaat gttcgttta ggggacgtg gttctagtg atgggagct ggtcccatt ggactcact tccctgctt gcagcaaga gcttatggt gcctctgct gttcttata acagatttg gcagaacat cgcttgaat aaagcgaga gaacttgga gctgattgc gtccttaaa gttgagaaa aatatgcga gaagaagag cttgtgaag gagattaaa tgcttgctg agggtggac ccaaatatt acaatagaa tgtacaggg gaggaatct agcattaga gcttccctc caggttaca aaaactgga ggtgttgtg gttttagtc ggtttggga aaatttgac ctaaatcta ccaattttt cccttattt cgggaggtt gatgttaga gggatattt cgctataat aatgactat ccgcaagct atagaaatg gtgcagagt ggaaaagcg aatgtgaag cccctaatt acacatcat tttgcaatg gaagacacg gttaaagcg ttcgagacc gcaagaact ggggcaggg aatccaatt aaaatcctg attcatgcc aatccagac tggaagatt tgtgaataa acaaatatg tatttattt tcccgaagt gttttttat taaattaag cgtgcgcgt tcgcgagag agacggcag gattctgtg gagaaacgc cagaattca atttccaat cggatcaat ggccgatga ccgatcaga ggctaaata acatctgat ttggagcac caggagaat ataaggagg tggaggtaa ccgttaagc cgccaatat ttccgatag gttcacatc aatcgattt tatgctcgc ttggcgcgc gataaattg tttattaaa tttggcacg atttcaaat tatttccag aacggacgc tctgtgatg cgagggatt atgttacta aaacttcat tccgctc
