| General information | |||
| TC001128 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 11359412..11410712 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC001128 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00216 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
- >TC001128 RA
MGANVSRHS SKGLSGRRS QSSGSICNG KGRHNDGKM CGSLPSYLD DRNSNGCNN NHHDCEKPP LDEVQRIPP LGDGAPLHW KFSDNQTVL CDQGYGSER SPEEEYPPP LPDHDTQCH HHLEPHACY PFITPESTF IVKLTKSSR GLGLSVTGG IDSGGSWPG LIRIKRLFP HQPASACGL LNVGDLIIE ANGITLTGL TNYEALEVL RTAPNQVDL KVCRPPPDV LSCVSPISE VPPPPPRRE PPTSLNLHP SEVYCTPED DYYHGEFEV TLTKIQGSL GFTLRKEDD SALGHYVRA LVREPALTD GRIRAGDKI IAVNEVEIS PMSHEQAVQ FLRQCGDVV KLRLYRDSA QTPVAALSP TETTPRTSF SKKTHLRQE AVDMLNDIA VRKLIPANH ATYKCSTSP TASPRRLRR QACPSDASQ MTDTSIKYL INDEQFHEG LLQKCPYDD DSLNLFTID PDDGDRPSR PSSLDLYNP NQTPVAARK PRFNFSLAH NAYELNNLD ADVLDAPNL TYNLGNEDV KSTEVECGD SFPKEPASM PHIPCDNQG FSYKNPVYQ SAHPPCGTE LEEKAASGQ KTSKTDDTN MKKWKGIVF DGHDEREEI PSMPIREEY EILAIELNR GWNSRLGFS LQGAAGVTY VSAVHADSV AARDGRLKP GDRVIKVND ESVEHLSTT EIIDLLRIV RGPVCIVVS RIKSKEDCP KSNAEN
- >TC001128 RA
atgggggcc aacgtcagc cgtcactcg tccaagggt ctctccgga cgacggtcc caatccagt ggcagcata tgcaacgga aagggaaga cacaacgac ggcaagatg tgtggcagt cttccgagc tacttggac gaccgcaac tccaacggc tgcaataac aaccatcac gactgcgag aaaccgcct cttgatgag gtgcagagg ataccgcct ctgggggat ggggcgccg cttcactgg aagttttca gataaccag acggtgctc tgcgaccag ggctacggc tcggaacgt tcccccgag gaggaatat ccgccgccc ttgcccgac catgacact caatgtcac caccacctg gagccccac gcttgctac cctttcatc acaccagaa agtactttc attgtgaaa ctgaccaag agttcaaga ggtcttggt ctgagcgtg actggtggt attgacagc ggcggctcg tggcctggc ctaatacga atcaaaaga ctgtttcca caccagccg gcgtcggct tgtgggctc ttaaacgta ggggatctc ataatagaa gctaacgga attacgctt accggcctt acaaattac gaggcttta gaagtatta aggactgca cccaaccaa gtcgacttg aaggtgtgc cgccctcct ccagacgtc ttaagctgt gtcagcccc atatctgaa gttcctccg ccgcctccg cgccgcgag ccgcccact agtcttaac ttgcacccg tcggaagtc tactgtact cccgaagac gactattac cacggtgag ttcgaagta acgctgacc aaaatacag ggatctcta ggatttacg ttgcggaaa gaggacgat agtgcgtta ggacattat gtgagggct cttgtccgg gagcctgca ctcacggat ggacggatt cgcgcaggg gacaaaatt attgccgta aatgaggtt gaaatatcg ccgatgtcg catgaacag gcggtgcag tttcttcga cagtgtggg gacgtcgtc aagttgcgg ctgtatcgc gactccgct cagacgccg gtggcagcg ctgtcgccg acggaaacc acgccgcga acttcgttc tcaaagaaa acacatctg agacaagaa gctgtggac atgctgaac gacatagca gtccgtaag ctgattccc gcaaaccac gcaacttac aaatgctcg acgtctccg acggcctca cctcgccgc ctccgtcgg caagcctgc ccttcggac gcctcccaa atgaccgac acctcgata aaatacctg ataaacgac gaacaattc cacgaaggc ctcctccag aagtgcccc tacgacgac gacagcttg aaccttttc accatagac cccgacgac ggcgaccgg ccgtcgcgc cccagctcc ctcgacctc tacaacccc aaccaaacg cccgtcgcg gcccgaaaa ccccgattc aacttctcc ctcgcccac aacgcttac gaactgaac aatctcgat gcagacgtg ctagatgcc ccgaatttg acgtacaat ttgggcaac gaagacgtc aaaagcacg gaagtcgaa tgcggcgac agcttcccg aaggaacca gcgagtatg ccgcacatt ccgtgcgac aatcagggc tttagctac aaaaacccc gtctatcag tccgctcat ccgccatgc gggacggaa ctagaggaa aaggccgct tcaggccag aaaactagc aaaacggac gacaccaac atgaagaag tggaaaggg attgttttt gacgggcat gacgagaga gaggagatc ccctcgatg ccaatacgg gaagaatat gaaattctc gcgatagag ctgaacagg ggatggaac agcaggttg ggcttcagc ttgcagggg gctgccggg gtgacgtac gtgagtgcc gtgcatgcg gacagtgtg gcagcgaga gacgggagg ttgaagccg ggcgatcgc gtgattaag gtgaatgac gagagcgta gagcatttg agtaccact gaaattata gacttgctt cgaatagtg agaggtcca gtttgtata gttgtatcc agaattaaa tcaaaagaa gactgccca aaaagcaat gctgagaat tga
- >TC001128 RA
gattattta cctcactta ccggagaaa cttttcgaa tagcggtat ccaactctt tcgccctaa aaccacccg acgccagtc ctcgggtca gtttgatca atcacccgg attttggtg gacggcgcc cttatctgg gcccataag gccagatat gggcagccg gcgcttccg ggggtccac ctgcagagg aaagctcgt cgatccggg agcgtgatc gtcgcctac aaccctgac gagctcgca agcacccca gtggattgc gctggcgca cgaccggcc ggtcgacct cgcctcttc accttgttc catcatcaa gcgagtttg cgagagata gtgtgctaa cgcggtcca tacaccgaa ctttcgtcg tcgaaacga caaattgca gtcgtatgt ggccgttaa cgataacgt gtgacggga tttggtgcc gcatgccgg cctagaacc cacacaggt acgtgctgc tttctgttc agtgtatca caacggctg ggttttggt gattttcaa ttattgccg ccgtgtgaa cttggatcg ctcaaatta aaggtcggt ttttgccca tgaacggct ggattgccc ccggtgtag ggtccgact acgctccca gcaaacgtg acagcacgt gtgtccaaa gagcaccca aaggacgcc gtcgacggc agcggtgat tccgacttg ctgcagctg gtgcgtcgt ctctcttcg agcgatcat gtgaagatg ggggccaac gtcagccgt cactcgtcc aagggtctc tccggacga cggtcccaa tccagtggc agcatatgc aacggaaag ggaagacac aacgacggc aagatgtgt ggcagtctt ccgagctac ttggacgac cgcaactcc aacggctgc aataacaac catcacgac tgcgagaaa ccgcctctt gatgaggtg cagaggata ccgcctctg ggggatggg gcgccgctt cactggaag ttttcagat aaccagacg gtgctctgc gaccagggc tacggctcg gaacgttcc cccgaggag gaatatccg ccgcccttg cccgaccat gacactcaa tgtcaccac cacctggag ccccacgct tgctaccct ttcatcaca ccagaaagt actttcatt gtgaaactg accaagagt tcaagaggt cttggtctg agcgtgact ggtggtatt gacagcggc ggctcgtgg cctggccta atacgaatc aaaagactg tttccacac cagccggcg tcggcttgt gggctctta aacgtaggg gatctcata atagaagct aacggaatt acgcttacc ggccttaca aattacgag gctttagaa gtattaagg actgcaccc aaccaagtc gacttgaag gtgtgccgc cctcctcca gacgtctta agctgtgtc agccccata tctgaagtt cctccgccg cctccgcgc cgcgagccg cccactagt cttaacttg cacccgtcg gaagtctac tgtactccc gaagacgac tattaccac ggtgagttc gaagtaacg ctgaccaaa atacaggga tctctagga tttacgttg cggaaagag gacgatagt gcgttagga cattatgtg agggctctt gtccgggag cctgcactc acggatgga cggattcgc gcaggggac aaaattatt gccgtaaat gaggttgaa atatcgccg atgtcgcat gaacaggcg gtgcagttt cttcgacag tgtggggac gtcgtcaag ttgcggctg tatcgcgac tccgctcag acgccggtg gcagcgctg tcgccgacg gaaaccacg ccgcgaact tcgttctca aagaaaaca catctgaga caagaagct gtggacatg ctgaacgac atagcagtc cgtaagctg attcccgca aaccacgca acttacaaa tgctcgacg tctccgacg gcctcacct cgccgcctc cgtcggcaa gcctgccct tcggacgcc tcccaaatg accgacacc tcgataaaa tacctgata aacgacgaa caattccac gaaggcctc ctccagaag tgcccctac gacgacgac agcttgaac cttttcacc atagacccc gacgacggc gaccggccg tcgcgcccc agctccctc gacctctac aaccccaac caaacgccc gtcgcggcc cgaaaaccc cgattcaac ttctccctc gcccacaac gcttacgaa ctgaacaat ctcgatgca gacgtgcta gatgccccg aatttgacg tacaatttg ggcaacgaa gacgtcaaa agcacggaa gtcgaatgc ggcgacagc ttcccgaag gaaccagcg agtatgccg cacattccg tgcgacaat cagggcttt agctacaaa aaccccgtc tatcagtcc gctcatccg ccatgcggg acggaacta gaggaaaag gccgcttca ggccagaaa actagcaaa acggacgac accaacatg aagaagtgg aaagggatt gtttttgac gggcatgac gagagagag gagatcccc tcgatgcca atacgggaa gaatatgaa attctcgcg atagagctg aacagggga tggaacagc aggttgggc ttcagcttg cagggggct gccggggtg acgtacgtg agtgccgtg catgcggac agtgtggca gcgagagac gggaggttg aagccgggc gatcgcgtg attaaggtg aatgacgag agcgtagag catttgagt accactgaa attatagac ttgcttcga atagtgaga ggtccagtt tgtatagtt gtatccaga attaaatca aaagaagac tgcccaaaa agcaatgct gagaattga aacgtagta caagttata ttctaagag catctatca aatataaca ctaaatagg aaaattgat taaatatat tgcatgttg aaaaatgat tgagcgatt taggctatt ttaacaatt ttaaactcc ttgttttag taaagtaag gaaaagaat gtgatagtt tagtgacaa ttttattac ggttgtttt ttaaatatt tccctacgt aaaatgtga ttttttaaa tacgatatt tttgttagt ggttttttc tgtttccaa atgtgtaaa gtataatgt tcttgtatc tagaatttt aagctagtc ttgtattgt atatggtac accttacag acttattat ataagtcta gctagcatt acatgtagt taaaacgta ttaaaatta ctattagat gaatgttca acaagtaga atcaaagtg tacaacaaa tattccaat tttaaaaaa tgatattaa aattgtatt ctaaattct cgcctaaat agcaaatgt gtattcttt attttttgt aagataatg gtgaatcaa actataaat caattaaat tttagcaat acttgaaac taaaaaata tatctataa catttgtta ttcaaaccg tttcctttt cttgtgtta cacttttta atagaaatt tataaacaa ctcgttctt ttaatcaaa ccaaagagg aataaaatc gttgaaatg ac
