Status
Please wait ...
Gene information
General information
TC001128 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11359412..11410712
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001128
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00216

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001128 RA
      MGANVSRHS SKGLSGRRS QSSGSICNG KGRHNDGKM 
      CGSLPSYLD DRNSNGCNN NHHDCEKPP LDEVQRIPP 
      LGDGAPLHW KFSDNQTVL CDQGYGSER SPEEEYPPP 
      LPDHDTQCH HHLEPHACY PFITPESTF IVKLTKSSR 
      GLGLSVTGG IDSGGSWPG LIRIKRLFP HQPASACGL 
      LNVGDLIIE ANGITLTGL TNYEALEVL RTAPNQVDL 
      KVCRPPPDV LSCVSPISE VPPPPPRRE PPTSLNLHP 
      SEVYCTPED DYYHGEFEV TLTKIQGSL GFTLRKEDD 
      SALGHYVRA LVREPALTD GRIRAGDKI IAVNEVEIS 
      PMSHEQAVQ FLRQCGDVV KLRLYRDSA QTPVAALSP 
      TETTPRTSF SKKTHLRQE AVDMLNDIA VRKLIPANH 
      ATYKCSTSP TASPRRLRR QACPSDASQ MTDTSIKYL 
      INDEQFHEG LLQKCPYDD DSLNLFTID PDDGDRPSR 
      PSSLDLYNP NQTPVAARK PRFNFSLAH NAYELNNLD 
      ADVLDAPNL TYNLGNEDV KSTEVECGD SFPKEPASM 
      PHIPCDNQG FSYKNPVYQ SAHPPCGTE LEEKAASGQ 
      KTSKTDDTN MKKWKGIVF DGHDEREEI PSMPIREEY 
      EILAIELNR GWNSRLGFS LQGAAGVTY VSAVHADSV 
      AARDGRLKP GDRVIKVND ESVEHLSTT EIIDLLRIV 
      RGPVCIVVS RIKSKEDCP KSNAEN
    CDS sequence
    • >TC001128 RA
      atgggggcc aacgtcagc cgtcactcg tccaagggt 
      ctctccgga cgacggtcc caatccagt ggcagcata 
      tgcaacgga aagggaaga cacaacgac ggcaagatg 
      tgtggcagt cttccgagc tacttggac gaccgcaac 
      tccaacggc tgcaataac aaccatcac gactgcgag 
      aaaccgcct cttgatgag gtgcagagg ataccgcct 
      ctgggggat ggggcgccg cttcactgg aagttttca 
      gataaccag acggtgctc tgcgaccag ggctacggc 
      tcggaacgt tcccccgag gaggaatat ccgccgccc 
      ttgcccgac catgacact caatgtcac caccacctg 
      gagccccac gcttgctac cctttcatc acaccagaa 
      agtactttc attgtgaaa ctgaccaag agttcaaga 
      ggtcttggt ctgagcgtg actggtggt attgacagc 
      ggcggctcg tggcctggc ctaatacga atcaaaaga 
      ctgtttcca caccagccg gcgtcggct tgtgggctc 
      ttaaacgta ggggatctc ataatagaa gctaacgga 
      attacgctt accggcctt acaaattac gaggcttta 
      gaagtatta aggactgca cccaaccaa gtcgacttg 
      aaggtgtgc cgccctcct ccagacgtc ttaagctgt 
      gtcagcccc atatctgaa gttcctccg ccgcctccg 
      cgccgcgag ccgcccact agtcttaac ttgcacccg 
      tcggaagtc tactgtact cccgaagac gactattac 
      cacggtgag ttcgaagta acgctgacc aaaatacag 
      ggatctcta ggatttacg ttgcggaaa gaggacgat 
      agtgcgtta ggacattat gtgagggct cttgtccgg 
      gagcctgca ctcacggat ggacggatt cgcgcaggg 
      gacaaaatt attgccgta aatgaggtt gaaatatcg 
      ccgatgtcg catgaacag gcggtgcag tttcttcga 
      cagtgtggg gacgtcgtc aagttgcgg ctgtatcgc 
      gactccgct cagacgccg gtggcagcg ctgtcgccg 
      acggaaacc acgccgcga acttcgttc tcaaagaaa 
      acacatctg agacaagaa gctgtggac atgctgaac 
      gacatagca gtccgtaag ctgattccc gcaaaccac 
      gcaacttac aaatgctcg acgtctccg acggcctca 
      cctcgccgc ctccgtcgg caagcctgc ccttcggac 
      gcctcccaa atgaccgac acctcgata aaatacctg 
      ataaacgac gaacaattc cacgaaggc ctcctccag 
      aagtgcccc tacgacgac gacagcttg aaccttttc 
      accatagac cccgacgac ggcgaccgg ccgtcgcgc 
      cccagctcc ctcgacctc tacaacccc aaccaaacg 
      cccgtcgcg gcccgaaaa ccccgattc aacttctcc 
      ctcgcccac aacgcttac gaactgaac aatctcgat 
      gcagacgtg ctagatgcc ccgaatttg acgtacaat 
      ttgggcaac gaagacgtc aaaagcacg gaagtcgaa 
      tgcggcgac agcttcccg aaggaacca gcgagtatg 
      ccgcacatt ccgtgcgac aatcagggc tttagctac 
      aaaaacccc gtctatcag tccgctcat ccgccatgc 
      gggacggaa ctagaggaa aaggccgct tcaggccag 
      aaaactagc aaaacggac gacaccaac atgaagaag 
      tggaaaggg attgttttt gacgggcat gacgagaga 
      gaggagatc ccctcgatg ccaatacgg gaagaatat 
      gaaattctc gcgatagag ctgaacagg ggatggaac 
      agcaggttg ggcttcagc ttgcagggg gctgccggg 
      gtgacgtac gtgagtgcc gtgcatgcg gacagtgtg 
      gcagcgaga gacgggagg ttgaagccg ggcgatcgc 
      gtgattaag gtgaatgac gagagcgta gagcatttg 
      agtaccact gaaattata gacttgctt cgaatagtg 
      agaggtcca gtttgtata gttgtatcc agaattaaa 
      tcaaaagaa gactgccca aaaagcaat gctgagaat 
      tga
    mRNA sequence
    • >TC001128 RA
      gattattta cctcactta ccggagaaa cttttcgaa 
      tagcggtat ccaactctt tcgccctaa aaccacccg 
      acgccagtc ctcgggtca gtttgatca atcacccgg 
      attttggtg gacggcgcc cttatctgg gcccataag 
      gccagatat gggcagccg gcgcttccg ggggtccac 
      ctgcagagg aaagctcgt cgatccggg agcgtgatc 
      gtcgcctac aaccctgac gagctcgca agcacccca 
      gtggattgc gctggcgca cgaccggcc ggtcgacct 
      cgcctcttc accttgttc catcatcaa gcgagtttg 
      cgagagata gtgtgctaa cgcggtcca tacaccgaa 
      ctttcgtcg tcgaaacga caaattgca gtcgtatgt 
      ggccgttaa cgataacgt gtgacggga tttggtgcc 
      gcatgccgg cctagaacc cacacaggt acgtgctgc 
      tttctgttc agtgtatca caacggctg ggttttggt 
      gattttcaa ttattgccg ccgtgtgaa cttggatcg 
      ctcaaatta aaggtcggt ttttgccca tgaacggct 
      ggattgccc ccggtgtag ggtccgact acgctccca 
      gcaaacgtg acagcacgt gtgtccaaa gagcaccca 
      aaggacgcc gtcgacggc agcggtgat tccgacttg 
      ctgcagctg gtgcgtcgt ctctcttcg agcgatcat 
      gtgaagatg ggggccaac gtcagccgt cactcgtcc 
      aagggtctc tccggacga cggtcccaa tccagtggc 
      agcatatgc aacggaaag ggaagacac aacgacggc 
      aagatgtgt ggcagtctt ccgagctac ttggacgac 
      cgcaactcc aacggctgc aataacaac catcacgac 
      tgcgagaaa ccgcctctt gatgaggtg cagaggata 
      ccgcctctg ggggatggg gcgccgctt cactggaag 
      ttttcagat aaccagacg gtgctctgc gaccagggc 
      tacggctcg gaacgttcc cccgaggag gaatatccg 
      ccgcccttg cccgaccat gacactcaa tgtcaccac 
      cacctggag ccccacgct tgctaccct ttcatcaca 
      ccagaaagt actttcatt gtgaaactg accaagagt 
      tcaagaggt cttggtctg agcgtgact ggtggtatt 
      gacagcggc ggctcgtgg cctggccta atacgaatc 
      aaaagactg tttccacac cagccggcg tcggcttgt 
      gggctctta aacgtaggg gatctcata atagaagct 
      aacggaatt acgcttacc ggccttaca aattacgag 
      gctttagaa gtattaagg actgcaccc aaccaagtc 
      gacttgaag gtgtgccgc cctcctcca gacgtctta 
      agctgtgtc agccccata tctgaagtt cctccgccg 
      cctccgcgc cgcgagccg cccactagt cttaacttg 
      cacccgtcg gaagtctac tgtactccc gaagacgac 
      tattaccac ggtgagttc gaagtaacg ctgaccaaa 
      atacaggga tctctagga tttacgttg cggaaagag 
      gacgatagt gcgttagga cattatgtg agggctctt 
      gtccgggag cctgcactc acggatgga cggattcgc 
      gcaggggac aaaattatt gccgtaaat gaggttgaa 
      atatcgccg atgtcgcat gaacaggcg gtgcagttt 
      cttcgacag tgtggggac gtcgtcaag ttgcggctg 
      tatcgcgac tccgctcag acgccggtg gcagcgctg 
      tcgccgacg gaaaccacg ccgcgaact tcgttctca 
      aagaaaaca catctgaga caagaagct gtggacatg 
      ctgaacgac atagcagtc cgtaagctg attcccgca 
      aaccacgca acttacaaa tgctcgacg tctccgacg 
      gcctcacct cgccgcctc cgtcggcaa gcctgccct 
      tcggacgcc tcccaaatg accgacacc tcgataaaa 
      tacctgata aacgacgaa caattccac gaaggcctc 
      ctccagaag tgcccctac gacgacgac agcttgaac 
      cttttcacc atagacccc gacgacggc gaccggccg 
      tcgcgcccc agctccctc gacctctac aaccccaac 
      caaacgccc gtcgcggcc cgaaaaccc cgattcaac 
      ttctccctc gcccacaac gcttacgaa ctgaacaat 
      ctcgatgca gacgtgcta gatgccccg aatttgacg 
      tacaatttg ggcaacgaa gacgtcaaa agcacggaa 
      gtcgaatgc ggcgacagc ttcccgaag gaaccagcg 
      agtatgccg cacattccg tgcgacaat cagggcttt 
      agctacaaa aaccccgtc tatcagtcc gctcatccg 
      ccatgcggg acggaacta gaggaaaag gccgcttca 
      ggccagaaa actagcaaa acggacgac accaacatg 
      aagaagtgg aaagggatt gtttttgac gggcatgac 
      gagagagag gagatcccc tcgatgcca atacgggaa 
      gaatatgaa attctcgcg atagagctg aacagggga 
      tggaacagc aggttgggc ttcagcttg cagggggct 
      gccggggtg acgtacgtg agtgccgtg catgcggac 
      agtgtggca gcgagagac gggaggttg aagccgggc 
      gatcgcgtg attaaggtg aatgacgag agcgtagag 
      catttgagt accactgaa attatagac ttgcttcga 
      atagtgaga ggtccagtt tgtatagtt gtatccaga 
      attaaatca aaagaagac tgcccaaaa agcaatgct 
      gagaattga aacgtagta caagttata ttctaagag 
      catctatca aatataaca ctaaatagg aaaattgat 
      taaatatat tgcatgttg aaaaatgat tgagcgatt 
      taggctatt ttaacaatt ttaaactcc ttgttttag 
      taaagtaag gaaaagaat gtgatagtt tagtgacaa 
      ttttattac ggttgtttt ttaaatatt tccctacgt 
      aaaatgtga ttttttaaa tacgatatt tttgttagt 
      ggttttttc tgtttccaa atgtgtaaa gtataatgt 
      tcttgtatc tagaatttt aagctagtc ttgtattgt 
      atatggtac accttacag acttattat ataagtcta 
      gctagcatt acatgtagt taaaacgta ttaaaatta 
      ctattagat gaatgttca acaagtaga atcaaagtg 
      tacaacaaa tattccaat tttaaaaaa tgatattaa 
      aattgtatt ctaaattct cgcctaaat agcaaatgt 
      gtattcttt attttttgt aagataatg gtgaatcaa 
      actataaat caattaaat tttagcaat acttgaaac 
      taaaaaata tatctataa catttgtta ttcaaaccg 
      tttcctttt cttgtgtta cacttttta atagaaatt 
      tataaacaa ctcgttctt ttaatcaaa ccaaagagg 
      aataaaatc gttgaaatg ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY