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Gene information
General information
TC001128 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11359412..11410712
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001128
    iBeetle screen iB_00216

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001128 RA
      MGANVSRHS SKGLSGRRS QSSGSICNG KGRHNDGKM 
CGSLPSYLD DRNSNGCNN NHHDCEKPP LDEVQRIPP 
LGDGAPLHW KFSDNQTVL CDQGYGSER SPEEEYPPP 
LPDHDTQCH HHLEPHACY PFITPESTF IVKLTKSSR 
GLGLSVTGG IDSGGSWPG LIRIKRLFP HQPASACGL 
LNVGDLIIE ANGITLTGL TNYEALEVL RTAPNQVDL 
KVCRPPPDV LSCVSPISE VPPPPPRRE PPTSLNLHP 
SEVYCTPED DYYHGEFEV TLTKIQGSL GFTLRKEDD 
SALGHYVRA LVREPALTD GRIRAGDKI IAVNEVEIS 
PMSHEQAVQ FLRQCGDVV KLRLYRDSA QTPVAALSP 
TETTPRTSF SKKTHLRQE AVDMLNDIA VRKLIPANH 
ATYKCSTSP TASPRRLRR QACPSDASQ MTDTSIKYL 
INDEQFHEG LLQKCPYDD DSLNLFTID PDDGDRPSR 
PSSLDLYNP NQTPVAARK PRFNFSLAH NAYELNNLD 
ADVLDAPNL TYNLGNEDV KSTEVECGD SFPKEPASM 
PHIPCDNQG FSYKNPVYQ SAHPPCGTE LEEKAASGQ 
KTSKTDDTN MKKWKGIVF DGHDEREEI PSMPIREEY 
EILAIELNR GWNSRLGFS LQGAAGVTY VSAVHADSV 
AARDGRLKP GDRVIKVND ESVEHLSTT EIIDLLRIV 
RGPVCIVVS RIKSKEDCP KSNAEN
    CDS sequence
    • >TC001128 RA
      atgggggcc aacgtcagc cgtcactcg tccaagggt 
ctctccgga cgacggtcc caatccagt ggcagcata 
tgcaacgga aagggaaga cacaacgac ggcaagatg 
tgtggcagt cttccgagc tacttggac gaccgcaac 
tccaacggc tgcaataac aaccatcac gactgcgag 
aaaccgcct cttgatgag gtgcagagg ataccgcct 
ctgggggat ggggcgccg cttcactgg aagttttca 
gataaccag acggtgctc tgcgaccag ggctacggc 
tcggaacgt tcccccgag gaggaatat ccgccgccc 
ttgcccgac catgacact caatgtcac caccacctg 
gagccccac gcttgctac cctttcatc acaccagaa 
agtactttc attgtgaaa ctgaccaag agttcaaga 
ggtcttggt ctgagcgtg actggtggt attgacagc 
ggcggctcg tggcctggc ctaatacga atcaaaaga 
ctgtttcca caccagccg gcgtcggct tgtgggctc 
ttaaacgta ggggatctc ataatagaa gctaacgga 
attacgctt accggcctt acaaattac gaggcttta 
gaagtatta aggactgca cccaaccaa gtcgacttg 
aaggtgtgc cgccctcct ccagacgtc ttaagctgt 
gtcagcccc atatctgaa gttcctccg ccgcctccg 
cgccgcgag ccgcccact agtcttaac ttgcacccg 
tcggaagtc tactgtact cccgaagac gactattac 
cacggtgag ttcgaagta acgctgacc aaaatacag 
ggatctcta ggatttacg ttgcggaaa gaggacgat 
agtgcgtta ggacattat gtgagggct cttgtccgg 
gagcctgca ctcacggat ggacggatt cgcgcaggg 
gacaaaatt attgccgta aatgaggtt gaaatatcg 
ccgatgtcg catgaacag gcggtgcag tttcttcga 
cagtgtggg gacgtcgtc aagttgcgg ctgtatcgc 
gactccgct cagacgccg gtggcagcg ctgtcgccg 
acggaaacc acgccgcga acttcgttc tcaaagaaa 
acacatctg agacaagaa gctgtggac atgctgaac 
gacatagca gtccgtaag ctgattccc gcaaaccac 
gcaacttac aaatgctcg acgtctccg acggcctca 
cctcgccgc ctccgtcgg caagcctgc ccttcggac 
gcctcccaa atgaccgac acctcgata aaatacctg 
ataaacgac gaacaattc cacgaaggc ctcctccag 
aagtgcccc tacgacgac gacagcttg aaccttttc 
accatagac cccgacgac ggcgaccgg ccgtcgcgc 
cccagctcc ctcgacctc tacaacccc aaccaaacg 
cccgtcgcg gcccgaaaa ccccgattc aacttctcc 
ctcgcccac aacgcttac gaactgaac aatctcgat 
gcagacgtg ctagatgcc ccgaatttg acgtacaat 
ttgggcaac gaagacgtc aaaagcacg gaagtcgaa 
tgcggcgac agcttcccg aaggaacca gcgagtatg 
ccgcacatt ccgtgcgac aatcagggc tttagctac 
aaaaacccc gtctatcag tccgctcat ccgccatgc 
gggacggaa ctagaggaa aaggccgct tcaggccag 
aaaactagc aaaacggac gacaccaac atgaagaag 
tggaaaggg attgttttt gacgggcat gacgagaga 
gaggagatc ccctcgatg ccaatacgg gaagaatat 
gaaattctc gcgatagag ctgaacagg ggatggaac 
agcaggttg ggcttcagc ttgcagggg gctgccggg 
gtgacgtac gtgagtgcc gtgcatgcg gacagtgtg 
gcagcgaga gacgggagg ttgaagccg ggcgatcgc 
gtgattaag gtgaatgac gagagcgta gagcatttg 
agtaccact gaaattata gacttgctt cgaatagtg 
agaggtcca gtttgtata gttgtatcc agaattaaa 
tcaaaagaa gactgccca aaaagcaat gctgagaat 
tga
    mRNA sequence
    • >TC001128 RA
      gattattta cctcactta ccggagaaa cttttcgaa 
tagcggtat ccaactctt tcgccctaa aaccacccg 
acgccagtc ctcgggtca gtttgatca atcacccgg 
attttggtg gacggcgcc cttatctgg gcccataag 
gccagatat gggcagccg gcgcttccg ggggtccac 
ctgcagagg aaagctcgt cgatccggg agcgtgatc 
gtcgcctac aaccctgac gagctcgca agcacccca 
gtggattgc gctggcgca cgaccggcc ggtcgacct 
cgcctcttc accttgttc catcatcaa gcgagtttg 
cgagagata gtgtgctaa cgcggtcca tacaccgaa 
ctttcgtcg tcgaaacga caaattgca gtcgtatgt 
ggccgttaa cgataacgt gtgacggga tttggtgcc 
gcatgccgg cctagaacc cacacaggt acgtgctgc 
tttctgttc agtgtatca caacggctg ggttttggt 
gattttcaa ttattgccg ccgtgtgaa cttggatcg 
ctcaaatta aaggtcggt ttttgccca tgaacggct 
ggattgccc ccggtgtag ggtccgact acgctccca 
gcaaacgtg acagcacgt gtgtccaaa gagcaccca 
aaggacgcc gtcgacggc agcggtgat tccgacttg 
ctgcagctg gtgcgtcgt ctctcttcg agcgatcat 
gtgaagatg ggggccaac gtcagccgt cactcgtcc 
aagggtctc tccggacga cggtcccaa tccagtggc 
agcatatgc aacggaaag ggaagacac aacgacggc 
aagatgtgt ggcagtctt ccgagctac ttggacgac 
cgcaactcc aacggctgc aataacaac catcacgac 
tgcgagaaa ccgcctctt gatgaggtg cagaggata 
ccgcctctg ggggatggg gcgccgctt cactggaag 
ttttcagat aaccagacg gtgctctgc gaccagggc 
tacggctcg gaacgttcc cccgaggag gaatatccg 
ccgcccttg cccgaccat gacactcaa tgtcaccac 
cacctggag ccccacgct tgctaccct ttcatcaca 
ccagaaagt actttcatt gtgaaactg accaagagt 
tcaagaggt cttggtctg agcgtgact ggtggtatt 
gacagcggc ggctcgtgg cctggccta atacgaatc 
aaaagactg tttccacac cagccggcg tcggcttgt 
gggctctta aacgtaggg gatctcata atagaagct 
aacggaatt acgcttacc ggccttaca aattacgag 
gctttagaa gtattaagg actgcaccc aaccaagtc 
gacttgaag gtgtgccgc cctcctcca gacgtctta 
agctgtgtc agccccata tctgaagtt cctccgccg 
cctccgcgc cgcgagccg cccactagt cttaacttg 
cacccgtcg gaagtctac tgtactccc gaagacgac 
tattaccac ggtgagttc gaagtaacg ctgaccaaa 
atacaggga tctctagga tttacgttg cggaaagag 
gacgatagt gcgttagga cattatgtg agggctctt 
gtccgggag cctgcactc acggatgga cggattcgc 
gcaggggac aaaattatt gccgtaaat gaggttgaa 
atatcgccg atgtcgcat gaacaggcg gtgcagttt 
cttcgacag tgtggggac gtcgtcaag ttgcggctg 
tatcgcgac tccgctcag acgccggtg gcagcgctg 
tcgccgacg gaaaccacg ccgcgaact tcgttctca 
aagaaaaca catctgaga caagaagct gtggacatg 
ctgaacgac atagcagtc cgtaagctg attcccgca 
aaccacgca acttacaaa tgctcgacg tctccgacg 
gcctcacct cgccgcctc cgtcggcaa gcctgccct 
tcggacgcc tcccaaatg accgacacc tcgataaaa 
tacctgata aacgacgaa caattccac gaaggcctc 
ctccagaag tgcccctac gacgacgac agcttgaac 
cttttcacc atagacccc gacgacggc gaccggccg 
tcgcgcccc agctccctc gacctctac aaccccaac 
caaacgccc gtcgcggcc cgaaaaccc cgattcaac 
ttctccctc gcccacaac gcttacgaa ctgaacaat 
ctcgatgca gacgtgcta gatgccccg aatttgacg 
tacaatttg ggcaacgaa gacgtcaaa agcacggaa 
gtcgaatgc ggcgacagc ttcccgaag gaaccagcg 
agtatgccg cacattccg tgcgacaat cagggcttt 
agctacaaa aaccccgtc tatcagtcc gctcatccg 
ccatgcggg acggaacta gaggaaaag gccgcttca 
ggccagaaa actagcaaa acggacgac accaacatg 
aagaagtgg aaagggatt gtttttgac gggcatgac 
gagagagag gagatcccc tcgatgcca atacgggaa 
gaatatgaa attctcgcg atagagctg aacagggga 
tggaacagc aggttgggc ttcagcttg cagggggct 
gccggggtg acgtacgtg agtgccgtg catgcggac 
agtgtggca gcgagagac gggaggttg aagccgggc 
gatcgcgtg attaaggtg aatgacgag agcgtagag 
catttgagt accactgaa attatagac ttgcttcga 
atagtgaga ggtccagtt tgtatagtt gtatccaga 
attaaatca aaagaagac tgcccaaaa agcaatgct 
gagaattga aacgtagta caagttata ttctaagag 
catctatca aatataaca ctaaatagg aaaattgat 
taaatatat tgcatgttg aaaaatgat tgagcgatt 
taggctatt ttaacaatt ttaaactcc ttgttttag 
taaagtaag gaaaagaat gtgatagtt tagtgacaa 
ttttattac ggttgtttt ttaaatatt tccctacgt 
aaaatgtga ttttttaaa tacgatatt tttgttagt 
ggttttttc tgtttccaa atgtgtaaa gtataatgt 
tcttgtatc tagaatttt aagctagtc ttgtattgt 
atatggtac accttacag acttattat ataagtcta 
gctagcatt acatgtagt taaaacgta ttaaaatta 
ctattagat gaatgttca acaagtaga atcaaagtg 
tacaacaaa tattccaat tttaaaaaa tgatattaa 
aattgtatt ctaaattct cgcctaaat agcaaatgt 
gtattcttt attttttgt aagataatg gtgaatcaa 
actataaat caattaaat tttagcaat acttgaaac 
taaaaaata tatctataa catttgtta ttcaaaccg 
tttcctttt cttgtgtta cacttttta atagaaatt 
tataaacaa ctcgttctt ttaatcaaa ccaaagagg 
aataaaatc gttgaaatg ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY