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Gene information
General information
TC001110 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11097262..11100093
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001110
    iBeetle screen iB_00214

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001110 RA
      MADRKPKTD SPEAVIRPN PDIKKGENK EQEKTEGKD 
      EKPDKAKGK GEPKTETKE TRVRWLTRA MAEDFTRHD 
      VLFTTFREA VLYIIFVIA VTICTVGRR SASMFYITQ 
      ALKGQFLEK DFQTANEKD IKYYDIRSA TDFWHYTQS 
      LMLENFYWE HYYNPYNPV AKAKDDDKK ILFENKLLG 
      VPRIRQVKV KNDSCIIHE YFRRLFTSC YDLYGPTDE 
      DRAPFGLEA GTAWTYNTA EKTESIPFS GKISKYGGG 
      GFYLDLSTN NEDTAKLIR DLKENLWIT RGTRAIFID 
      FSIYNANLN LFCVCKLIF EFPPTGGII PSHSFHAVH 
      LVHYVNSWD YVTFVFECT VYAIAGFFL AEEIREIIY 
      FKLRYFLQF WTYIDVVII GMAFANLIT SIVVFPNVD 
      DAILKIQKN PQKYGNLEY LAEMRIFYN NFAASLLFF 
      SYIKLFKYL NFNKTMGQL NNTLKRCAF DILGFSIMF 
      FIIFFAFAL LGYLLFGSQ VEDFSSFGV AMFTLLRTI 
      LGDFDYQAI EKANRVLAP IYFLAYIFF VFFVLLNMF 
      LAIINDTYA DVKTEIAIA PDEMQMTEF LKKGFYKML 
      QKCGCNIKY FQQQKAEFN ATIQQIRDA LKKCGFSDL 
      EIEMFFARY NIDPLAEVG DYDIKKIMK ELEGQSMAK 
      EKVDEDTTL VHVSDFITQ QERLDQIEK TIQMLATKI 
      DTLIKKLEA LENVRKAKA TQG
    CDS sequence
    • >TC001110 RA
      atggccgat cgcaaacct aaaacagac tccccagag 
      gctgtaatt aggccaaat ccggatata aaaaaagga 
      gaaaataaa gaacaggag aaaaccgaa gggaaggat 
      gagaaaccc gacaaagca aagggaaaa ggagagccc 
      aagactgaa actaaggag actagagtt cgatggttg 
      actcgagca atggctgag gatttcacc cgacatgat 
      gtgctattc accacgttt cgggaagca gtgctttac 
      attattttc gtgattgct gtgacgatt tgcacggtt 
      gggagacgg tccgcttct atgttttac atcacccaa 
      gccctgaaa ggccagttt ttggaaaaa gactttcaa 
      acagctaat gaaaaagat attaagtat tacgatatt 
      aggtcggcg actgatttt tggcattat acgcagtct 
      ttaatgctg gaaaacttc tattgggaa cattactac 
      aatccgtac aatcctgtg gcaaaagcc aaagatgac 
      gacaagaag attcttttc gaaaacaag ttgctggga 
      gttccccga ataagacaa gtcaaagtg aagaatgat 
      tcgtgcata atacatgaa tatttccga cgcttattt 
      acatcatgc tacgatttg tacggacct actgacgaa 
      gatcgggca ccctttggc ctggaagcc ggaacagca 
      tggacctac aacacagct gaaaaaact gaaagcatc 
      cctttttct ggaaaaatt tcaaagtac gggggcggc 
      ggcttttac ttagatttg tcgacaaac aacgaggac 
      acggccaag ttaattcga gatttgaaa gaaaacttg 
      tggataact cgaggcacc cgagcgatt ttcatcgat 
      ttctccatc tataacgca aatttaaat ttattttgc 
      gtctgcaaa ttaattttt gagtttcca cccacgggt 
      ggaataatc ccgtcgcat tctttccac gcagtgcat 
      ctagtccac tacgtaaac agttgggat tacgttact 
      ttcgttttt gagtgtact gtttatgca atcgctggc 
      tttttcctg gccgaagag atcagggaa ataatttat 
      ttcaaattg cgctatttt ctgcaattt tggacctat 
      atcgatgtc gttatcata ggcatggct ttcgcgaat 
      cttatcact tcgattgtg gtattcccg aatgtggat 
      gacgcgatt ttgaaaata caaaagaat ccgcaaaag 
      tacggaaat ctggaatat ctggccgag atgaggatt 
      ttttacaac aattttgcc gcgtccctg ctcttcttt 
      tcctacatc aagttgttc aaatacctc aactttaac 
      aaaaccatg gggcaactc aacaatacg ctgaaacgg 
      tgcgcattc gacattttg ggcttctcg attatgttc 
      ttcattata ttttttgcg tttgctttg ttgggatat 
      ttgctgttt gggagccaa gtcgaggac tttagcagt 
      tttggggtg gccatgttc acattattg cggacaatt 
      ttgggcgat tttgactac caagcgatc gaaaaagcc 
      aacagagtt ctggcgccg atttatttc cttgcctac 
      attttcttc gtgttcttc gtgctattg aacatgttt 
      ttggccatt attaacgac acttatgcc gatgtcaag 
      accgagatt gccatagca cctgatgag atgcaaatg 
      accgagttt ttgaagaaa gggttctac aaaatgttg 
      cagaaatgc ggttgcaat ataaaatat ttccagcag 
      caaaaagcc gaatttaat gccacaatt caacaaatc 
      cgcgatgca cttaaaaag tgtggtttt agcgacctg 
      gaaatagaa atgtttttt gcacggtat aacatcgat 
      cctttggct gaagttggc gattatgat attaaaaaa 
      atcatgaag gaattggaa ggacagtcg atggctaag 
      gaaaaggtg gatgaagac acgactctt gtgcacgtc 
      agcgatttt ataacgcaa caagaaaga ctcgaccag 
      attgagaaa accattcaa atgcttgcc acaaagata 
      gatacgtta attaaaaaa ctggaagca cttgaaaat 
      gtgaggaaa gctaaggca acgcaaggc tga
    mRNA sequence
    • >TC001110 RA
      atagagata aaataaata aacgagaag cagccaaat 
      atcaattta attttcctt gcaaaaatg gccgatcgc 
      aaacctaaa acagactcc ccagaggct gtaattagg 
      ccaaatccg gatataaaa aaaggagaa aataaagaa 
      caggagaaa accgaaggg aaggatgag aaacccgac 
      aaagcaaag ggaaaagga gagcccaag actgaaact 
      aaggagact agagttcga tggttgact cgagcaatg 
      gctgaggat ttcacccga catgatgtg ctattcacc 
      acgtttcgg gaagcagtg ctttacatt attttcgtg 
      attgctgtg acgatttgc acggttggg agacggtcc 
      gcttctatg ttttacatc acccaagcc ctgaaaggc 
      cagtttttg gaaaaagac tttcaaaca gctaatgaa 
      aaagatatt aagtattac gatattagg tcggcgact 
      gatttttgg cattatacg cagtcttta atgctggaa 
      aacttctat tgggaacat tactacaat ccgtacaat 
      cctgtggca aaagccaaa gatgacgac aagaagatt 
      cttttcgaa aacaagttg ctgggagtt ccccgaata 
      agacaagtc aaagtgaag aatgattcg tgcataata 
      catgaatat ttccgacgc ttatttaca tcatgctac 
      gatttgtac ggacctact gacgaagat cgggcaccc 
      tttggcctg gaagccgga acagcatgg acctacaac 
      acagctgaa aaaactgaa agcatccct ttttctgga 
      aaaatttca aagtacggg ggcggcggc ttttactta 
      gatttgtcg acaaacaac gaggacacg gccaagtta 
      attcgagat ttgaaagaa aacttgtgg ataactcga 
      ggcacccga gcgattttc atcgatttc tccatctat 
      aacgcaaat ttaaattta ttttgcgtc tgcaaatta 
      atttttgag tttccaccc acgggtgga ataatcccg 
      tcgcattct ttccacgca gtgcatcta gtccactac 
      gtaaacagt tgggattac gttactttc gtttttgag 
      tgtactgtt tatgcaatc gctggcttt ttcctggcc 
      gaagagatc agggaaata atttatttc aaattgcgc 
      tattttctg caattttgg acctatatc gatgtcgtt 
      atcataggc atggctttc gcgaatctt atcacttcg 
      attgtggta ttcccgaat gtggatgac gcgattttg 
      aaaatacaa aagaatccg caaaagtac ggaaatctg 
      gaatatctg gccgagatg aggattttt tacaacaat 
      tttgccgcg tccctgctc ttcttttcc tacatcaag 
      ttgttcaaa tacctcaac tttaacaaa accatgggg 
      caactcaac aatacgctg aaacggtgc gcattcgac 
      attttgggc ttctcgatt atgttcttc attatattt 
      tttgcgttt gctttgttg ggatatttg ctgtttggg 
      agccaagtc gaggacttt agcagtttt ggggtggcc 
      atgttcaca ttattgcgg acaattttg ggcgatttt 
      gactaccaa gcgatcgaa aaagccaac agagttctg 
      gcgccgatt tatttcctt gcctacatt ttcttcgtg 
      ttcttcgtg ctattgaac atgtttttg gccattatt 
      aacgacact tatgccgat gtcaagacc gagattgcc 
      atagcacct gatgagatg caaatgacc gagtttttg 
      aagaaaggg ttctacaaa atgttgcag aaatgcggt 
      tgcaatata aaatatttc cagcagcaa aaagccgaa 
      tttaatgcc acaattcaa caaatccgc gatgcactt 
      aaaaagtgt ggttttagc gacctggaa atagaaatg 
      ttttttgca cggtataac atcgatcct ttggctgaa 
      gttggcgat tatgatatt aaaaaaatc atgaaggaa 
      ttggaagga cagtcgatg gctaaggaa aaggtggat 
      gaagacacg actcttgtg cacgtcagc gattttata 
      acgcaacaa gaaagactc gaccagatt gagaaaacc 
      attcaaatg cttgccaca aagatagat acgttaatt 
      aaaaaactg gaagcactt gaaaatgtg aggaaagct 
      aaggcaacg caaggctga ttgtcagtt ttaagtact 
      ttgattaga agttttata ttttttgaa ttattgtta 
      ttgttcttt gacaataaa tatgattta taata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY