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Gene information
General information
TC001110 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 11097262..11100093
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001110
    iBeetle screen iB_00214

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001110 RA
      MADRKPKTD SPEAVIRPN PDIKKGENK EQEKTEGKD 
EKPDKAKGK GEPKTETKE TRVRWLTRA MAEDFTRHD 
VLFTTFREA VLYIIFVIA VTICTVGRR SASMFYITQ 
ALKGQFLEK DFQTANEKD IKYYDIRSA TDFWHYTQS 
LMLENFYWE HYYNPYNPV AKAKDDDKK ILFENKLLG 
VPRIRQVKV KNDSCIIHE YFRRLFTSC YDLYGPTDE 
DRAPFGLEA GTAWTYNTA EKTESIPFS GKISKYGGG 
GFYLDLSTN NEDTAKLIR DLKENLWIT RGTRAIFID 
FSIYNANLN LFCVCKLIF EFPPTGGII PSHSFHAVH 
LVHYVNSWD YVTFVFECT VYAIAGFFL AEEIREIIY 
FKLRYFLQF WTYIDVVII GMAFANLIT SIVVFPNVD 
DAILKIQKN PQKYGNLEY LAEMRIFYN NFAASLLFF 
SYIKLFKYL NFNKTMGQL NNTLKRCAF DILGFSIMF 
FIIFFAFAL LGYLLFGSQ VEDFSSFGV AMFTLLRTI 
LGDFDYQAI EKANRVLAP IYFLAYIFF VFFVLLNMF 
LAIINDTYA DVKTEIAIA PDEMQMTEF LKKGFYKML 
QKCGCNIKY FQQQKAEFN ATIQQIRDA LKKCGFSDL 
EIEMFFARY NIDPLAEVG DYDIKKIMK ELEGQSMAK 
EKVDEDTTL VHVSDFITQ QERLDQIEK TIQMLATKI 
DTLIKKLEA LENVRKAKA TQG
    CDS sequence
    • >TC001110 RA
      atggccgat cgcaaacct aaaacagac tccccagag 
gctgtaatt aggccaaat ccggatata aaaaaagga 
gaaaataaa gaacaggag aaaaccgaa gggaaggat 
gagaaaccc gacaaagca aagggaaaa ggagagccc 
aagactgaa actaaggag actagagtt cgatggttg 
actcgagca atggctgag gatttcacc cgacatgat 
gtgctattc accacgttt cgggaagca gtgctttac 
attattttc gtgattgct gtgacgatt tgcacggtt 
gggagacgg tccgcttct atgttttac atcacccaa 
gccctgaaa ggccagttt ttggaaaaa gactttcaa 
acagctaat gaaaaagat attaagtat tacgatatt 
aggtcggcg actgatttt tggcattat acgcagtct 
ttaatgctg gaaaacttc tattgggaa cattactac 
aatccgtac aatcctgtg gcaaaagcc aaagatgac 
gacaagaag attcttttc gaaaacaag ttgctggga 
gttccccga ataagacaa gtcaaagtg aagaatgat 
tcgtgcata atacatgaa tatttccga cgcttattt 
acatcatgc tacgatttg tacggacct actgacgaa 
gatcgggca ccctttggc ctggaagcc ggaacagca 
tggacctac aacacagct gaaaaaact gaaagcatc 
cctttttct ggaaaaatt tcaaagtac gggggcggc 
ggcttttac ttagatttg tcgacaaac aacgaggac 
acggccaag ttaattcga gatttgaaa gaaaacttg 
tggataact cgaggcacc cgagcgatt ttcatcgat 
ttctccatc tataacgca aatttaaat ttattttgc 
gtctgcaaa ttaattttt gagtttcca cccacgggt 
ggaataatc ccgtcgcat tctttccac gcagtgcat 
ctagtccac tacgtaaac agttgggat tacgttact 
ttcgttttt gagtgtact gtttatgca atcgctggc 
tttttcctg gccgaagag atcagggaa ataatttat 
ttcaaattg cgctatttt ctgcaattt tggacctat 
atcgatgtc gttatcata ggcatggct ttcgcgaat 
cttatcact tcgattgtg gtattcccg aatgtggat 
gacgcgatt ttgaaaata caaaagaat ccgcaaaag 
tacggaaat ctggaatat ctggccgag atgaggatt 
ttttacaac aattttgcc gcgtccctg ctcttcttt 
tcctacatc aagttgttc aaatacctc aactttaac 
aaaaccatg gggcaactc aacaatacg ctgaaacgg 
tgcgcattc gacattttg ggcttctcg attatgttc 
ttcattata ttttttgcg tttgctttg ttgggatat 
ttgctgttt gggagccaa gtcgaggac tttagcagt 
tttggggtg gccatgttc acattattg cggacaatt 
ttgggcgat tttgactac caagcgatc gaaaaagcc 
aacagagtt ctggcgccg atttatttc cttgcctac 
attttcttc gtgttcttc gtgctattg aacatgttt 
ttggccatt attaacgac acttatgcc gatgtcaag 
accgagatt gccatagca cctgatgag atgcaaatg 
accgagttt ttgaagaaa gggttctac aaaatgttg 
cagaaatgc ggttgcaat ataaaatat ttccagcag 
caaaaagcc gaatttaat gccacaatt caacaaatc 
cgcgatgca cttaaaaag tgtggtttt agcgacctg 
gaaatagaa atgtttttt gcacggtat aacatcgat 
cctttggct gaagttggc gattatgat attaaaaaa 
atcatgaag gaattggaa ggacagtcg atggctaag 
gaaaaggtg gatgaagac acgactctt gtgcacgtc 
agcgatttt ataacgcaa caagaaaga ctcgaccag 
attgagaaa accattcaa atgcttgcc acaaagata 
gatacgtta attaaaaaa ctggaagca cttgaaaat 
gtgaggaaa gctaaggca acgcaaggc tga
    mRNA sequence
    • >TC001110 RA
      atagagata aaataaata aacgagaag cagccaaat 
atcaattta attttcctt gcaaaaatg gccgatcgc 
aaacctaaa acagactcc ccagaggct gtaattagg 
ccaaatccg gatataaaa aaaggagaa aataaagaa 
caggagaaa accgaaggg aaggatgag aaacccgac 
aaagcaaag ggaaaagga gagcccaag actgaaact 
aaggagact agagttcga tggttgact cgagcaatg 
gctgaggat ttcacccga catgatgtg ctattcacc 
acgtttcgg gaagcagtg ctttacatt attttcgtg 
attgctgtg acgatttgc acggttggg agacggtcc 
gcttctatg ttttacatc acccaagcc ctgaaaggc 
cagtttttg gaaaaagac tttcaaaca gctaatgaa 
aaagatatt aagtattac gatattagg tcggcgact 
gatttttgg cattatacg cagtcttta atgctggaa 
aacttctat tgggaacat tactacaat ccgtacaat 
cctgtggca aaagccaaa gatgacgac aagaagatt 
cttttcgaa aacaagttg ctgggagtt ccccgaata 
agacaagtc aaagtgaag aatgattcg tgcataata 
catgaatat ttccgacgc ttatttaca tcatgctac 
gatttgtac ggacctact gacgaagat cgggcaccc 
tttggcctg gaagccgga acagcatgg acctacaac 
acagctgaa aaaactgaa agcatccct ttttctgga 
aaaatttca aagtacggg ggcggcggc ttttactta 
gatttgtcg acaaacaac gaggacacg gccaagtta 
attcgagat ttgaaagaa aacttgtgg ataactcga 
ggcacccga gcgattttc atcgatttc tccatctat 
aacgcaaat ttaaattta ttttgcgtc tgcaaatta 
atttttgag tttccaccc acgggtgga ataatcccg 
tcgcattct ttccacgca gtgcatcta gtccactac 
gtaaacagt tgggattac gttactttc gtttttgag 
tgtactgtt tatgcaatc gctggcttt ttcctggcc 
gaagagatc agggaaata atttatttc aaattgcgc 
tattttctg caattttgg acctatatc gatgtcgtt 
atcataggc atggctttc gcgaatctt atcacttcg 
attgtggta ttcccgaat gtggatgac gcgattttg 
aaaatacaa aagaatccg caaaagtac ggaaatctg 
gaatatctg gccgagatg aggattttt tacaacaat 
tttgccgcg tccctgctc ttcttttcc tacatcaag 
ttgttcaaa tacctcaac tttaacaaa accatgggg 
caactcaac aatacgctg aaacggtgc gcattcgac 
attttgggc ttctcgatt atgttcttc attatattt 
tttgcgttt gctttgttg ggatatttg ctgtttggg 
agccaagtc gaggacttt agcagtttt ggggtggcc 
atgttcaca ttattgcgg acaattttg ggcgatttt 
gactaccaa gcgatcgaa aaagccaac agagttctg 
gcgccgatt tatttcctt gcctacatt ttcttcgtg 
ttcttcgtg ctattgaac atgtttttg gccattatt 
aacgacact tatgccgat gtcaagacc gagattgcc 
atagcacct gatgagatg caaatgacc gagtttttg 
aagaaaggg ttctacaaa atgttgcag aaatgcggt 
tgcaatata aaatatttc cagcagcaa aaagccgaa 
tttaatgcc acaattcaa caaatccgc gatgcactt 
aaaaagtgt ggttttagc gacctggaa atagaaatg 
ttttttgca cggtataac atcgatcct ttggctgaa 
gttggcgat tatgatatt aaaaaaatc atgaaggaa 
ttggaagga cagtcgatg gctaaggaa aaggtggat 
gaagacacg actcttgtg cacgtcagc gattttata 
acgcaacaa gaaagactc gaccagatt gagaaaacc 
attcaaatg cttgccaca aagatagat acgttaatt 
aaaaaactg gaagcactt gaaaatgtg aggaaagct 
aaggcaacg caaggctga ttgtcagtt ttaagtact 
ttgattaga agttttata ttttttgaa ttattgtta 
ttgttcttt gacaataaa tatgattta taata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY