| General information | |||
| TC001110 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 11097262..11100093 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC001110 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00214 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001110 RA
MADRKPKTD SPEAVIRPN PDIKKGENK EQEKTEGKD EKPDKAKGK GEPKTETKE TRVRWLTRA MAEDFTRHD VLFTTFREA VLYIIFVIA VTICTVGRR SASMFYITQ ALKGQFLEK DFQTANEKD IKYYDIRSA TDFWHYTQS LMLENFYWE HYYNPYNPV AKAKDDDKK ILFENKLLG VPRIRQVKV KNDSCIIHE YFRRLFTSC YDLYGPTDE DRAPFGLEA GTAWTYNTA EKTESIPFS GKISKYGGG GFYLDLSTN NEDTAKLIR DLKENLWIT RGTRAIFID FSIYNANLN LFCVCKLIF EFPPTGGII PSHSFHAVH LVHYVNSWD YVTFVFECT VYAIAGFFL AEEIREIIY FKLRYFLQF WTYIDVVII GMAFANLIT SIVVFPNVD DAILKIQKN PQKYGNLEY LAEMRIFYN NFAASLLFF SYIKLFKYL NFNKTMGQL NNTLKRCAF DILGFSIMF FIIFFAFAL LGYLLFGSQ VEDFSSFGV AMFTLLRTI LGDFDYQAI EKANRVLAP IYFLAYIFF VFFVLLNMF LAIINDTYA DVKTEIAIA PDEMQMTEF LKKGFYKML QKCGCNIKY FQQQKAEFN ATIQQIRDA LKKCGFSDL EIEMFFARY NIDPLAEVG DYDIKKIMK ELEGQSMAK EKVDEDTTL VHVSDFITQ QERLDQIEK TIQMLATKI DTLIKKLEA LENVRKAKA TQG
- >TC001110 RA
atggccgat cgcaaacct aaaacagac tccccagag gctgtaatt aggccaaat ccggatata aaaaaagga gaaaataaa gaacaggag aaaaccgaa gggaaggat gagaaaccc gacaaagca aagggaaaa ggagagccc aagactgaa actaaggag actagagtt cgatggttg actcgagca atggctgag gatttcacc cgacatgat gtgctattc accacgttt cgggaagca gtgctttac attattttc gtgattgct gtgacgatt tgcacggtt gggagacgg tccgcttct atgttttac atcacccaa gccctgaaa ggccagttt ttggaaaaa gactttcaa acagctaat gaaaaagat attaagtat tacgatatt aggtcggcg actgatttt tggcattat acgcagtct ttaatgctg gaaaacttc tattgggaa cattactac aatccgtac aatcctgtg gcaaaagcc aaagatgac gacaagaag attcttttc gaaaacaag ttgctggga gttccccga ataagacaa gtcaaagtg aagaatgat tcgtgcata atacatgaa tatttccga cgcttattt acatcatgc tacgatttg tacggacct actgacgaa gatcgggca ccctttggc ctggaagcc ggaacagca tggacctac aacacagct gaaaaaact gaaagcatc cctttttct ggaaaaatt tcaaagtac gggggcggc ggcttttac ttagatttg tcgacaaac aacgaggac acggccaag ttaattcga gatttgaaa gaaaacttg tggataact cgaggcacc cgagcgatt ttcatcgat ttctccatc tataacgca aatttaaat ttattttgc gtctgcaaa ttaattttt gagtttcca cccacgggt ggaataatc ccgtcgcat tctttccac gcagtgcat ctagtccac tacgtaaac agttgggat tacgttact ttcgttttt gagtgtact gtttatgca atcgctggc tttttcctg gccgaagag atcagggaa ataatttat ttcaaattg cgctatttt ctgcaattt tggacctat atcgatgtc gttatcata ggcatggct ttcgcgaat cttatcact tcgattgtg gtattcccg aatgtggat gacgcgatt ttgaaaata caaaagaat ccgcaaaag tacggaaat ctggaatat ctggccgag atgaggatt ttttacaac aattttgcc gcgtccctg ctcttcttt tcctacatc aagttgttc aaatacctc aactttaac aaaaccatg gggcaactc aacaatacg ctgaaacgg tgcgcattc gacattttg ggcttctcg attatgttc ttcattata ttttttgcg tttgctttg ttgggatat ttgctgttt gggagccaa gtcgaggac tttagcagt tttggggtg gccatgttc acattattg cggacaatt ttgggcgat tttgactac caagcgatc gaaaaagcc aacagagtt ctggcgccg atttatttc cttgcctac attttcttc gtgttcttc gtgctattg aacatgttt ttggccatt attaacgac acttatgcc gatgtcaag accgagatt gccatagca cctgatgag atgcaaatg accgagttt ttgaagaaa gggttctac aaaatgttg cagaaatgc ggttgcaat ataaaatat ttccagcag caaaaagcc gaatttaat gccacaatt caacaaatc cgcgatgca cttaaaaag tgtggtttt agcgacctg gaaatagaa atgtttttt gcacggtat aacatcgat cctttggct gaagttggc gattatgat attaaaaaa atcatgaag gaattggaa ggacagtcg atggctaag gaaaaggtg gatgaagac acgactctt gtgcacgtc agcgatttt ataacgcaa caagaaaga ctcgaccag attgagaaa accattcaa atgcttgcc acaaagata gatacgtta attaaaaaa ctggaagca cttgaaaat gtgaggaaa gctaaggca acgcaaggc tga
- >TC001110 RA
atagagata aaataaata aacgagaag cagccaaat atcaattta attttcctt gcaaaaatg gccgatcgc aaacctaaa acagactcc ccagaggct gtaattagg ccaaatccg gatataaaa aaaggagaa aataaagaa caggagaaa accgaaggg aaggatgag aaacccgac aaagcaaag ggaaaagga gagcccaag actgaaact aaggagact agagttcga tggttgact cgagcaatg gctgaggat ttcacccga catgatgtg ctattcacc acgtttcgg gaagcagtg ctttacatt attttcgtg attgctgtg acgatttgc acggttggg agacggtcc gcttctatg ttttacatc acccaagcc ctgaaaggc cagtttttg gaaaaagac tttcaaaca gctaatgaa aaagatatt aagtattac gatattagg tcggcgact gatttttgg cattatacg cagtcttta atgctggaa aacttctat tgggaacat tactacaat ccgtacaat cctgtggca aaagccaaa gatgacgac aagaagatt cttttcgaa aacaagttg ctgggagtt ccccgaata agacaagtc aaagtgaag aatgattcg tgcataata catgaatat ttccgacgc ttatttaca tcatgctac gatttgtac ggacctact gacgaagat cgggcaccc tttggcctg gaagccgga acagcatgg acctacaac acagctgaa aaaactgaa agcatccct ttttctgga aaaatttca aagtacggg ggcggcggc ttttactta gatttgtcg acaaacaac gaggacacg gccaagtta attcgagat ttgaaagaa aacttgtgg ataactcga ggcacccga gcgattttc atcgatttc tccatctat aacgcaaat ttaaattta ttttgcgtc tgcaaatta atttttgag tttccaccc acgggtgga ataatcccg tcgcattct ttccacgca gtgcatcta gtccactac gtaaacagt tgggattac gttactttc gtttttgag tgtactgtt tatgcaatc gctggcttt ttcctggcc gaagagatc agggaaata atttatttc aaattgcgc tattttctg caattttgg acctatatc gatgtcgtt atcataggc atggctttc gcgaatctt atcacttcg attgtggta ttcccgaat gtggatgac gcgattttg aaaatacaa aagaatccg caaaagtac ggaaatctg gaatatctg gccgagatg aggattttt tacaacaat tttgccgcg tccctgctc ttcttttcc tacatcaag ttgttcaaa tacctcaac tttaacaaa accatgggg caactcaac aatacgctg aaacggtgc gcattcgac attttgggc ttctcgatt atgttcttc attatattt tttgcgttt gctttgttg ggatatttg ctgtttggg agccaagtc gaggacttt agcagtttt ggggtggcc atgttcaca ttattgcgg acaattttg ggcgatttt gactaccaa gcgatcgaa aaagccaac agagttctg gcgccgatt tatttcctt gcctacatt ttcttcgtg ttcttcgtg ctattgaac atgtttttg gccattatt aacgacact tatgccgat gtcaagacc gagattgcc atagcacct gatgagatg caaatgacc gagtttttg aagaaaggg ttctacaaa atgttgcag aaatgcggt tgcaatata aaatatttc cagcagcaa aaagccgaa tttaatgcc acaattcaa caaatccgc gatgcactt aaaaagtgt ggttttagc gacctggaa atagaaatg ttttttgca cggtataac atcgatcct ttggctgaa gttggcgat tatgatatt aaaaaaatc atgaaggaa ttggaagga cagtcgatg gctaaggaa aaggtggat gaagacacg actcttgtg cacgtcagc gattttata acgcaacaa gaaagactc gaccagatt gagaaaacc attcaaatg cttgccaca aagatagat acgttaatt aaaaaactg gaagcactt gaaaatgtg aggaaagct aaggcaacg caaggctga ttgtcagtt ttaagtact ttgattaga agttttata ttttttgaa ttattgtta ttgttcttt gacaataaa tatgattta taata
