| General information | |||
| TC034557 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 10295063..10299834 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC034557 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00210 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
- >TC034557 RA
MEVLVEHDY IAKEPNELT ITRGDIIKD VTKKQGGWW EGTLKDKKG LFPDNFVKV LDKDSSVVL RNRKDASRI RQCRVVFSY KQDHEDELN LNVGDIIDI LGEEEEGWW RGVLNGKEG VFPSNFVEE IAPLASKHN SSKENLTNS IASNETPPL YSKPTKILC EVKFPYKAQ NDDELTLKE GDLVVLISK DGQDPGWWK GELNGVVGV FPDNFVTVL PSAGDETPQ KDERRVKSL VEPHSIKHG TVSAQRKSL EVHNDKSEK LENENLSHT KTTPPLPGK KPTIPLKKS PSGSSSSGG IFQGLKKKL VDVVDGATG SKISPPKPE PCESALPEN VFDDVHRRP LLSDVRASR PKAPGRRPP SIIGKDQEP GLMNGNSDH NALETMSEA AFSVSSSSE GDPTESKPK LPEWAKHPA PWLEEMKLN QAKRSSFTP PQDKLRLTP TDKTDDEAK SKSFKKDPS PVDKSKSIA NARVKTPTN EFPAIRNKP NVPIPSKPQ TIADVFTPQ KPITKVSPA SNKTPSPMH RIDTNLDSS AGGDSGEEL TSKQYAELE HRIARLEEL FEKQRQNYE AAIEELRGK LQIETERRM ALEANFDKF LQDGAIQQL
- >TC034557 RB
MAEVLVEHD YIAKEPNEL TITRGDIIK DVTKKQGGW WEGTLKDKK GLFPDNFVK VLDKDSSVV LRNRKDASR IRQCRVVFS YKQDHEDEL NLNVGDIID ILGEEEEGW WRGVLNGKE GVFPSNFVE EIAPLASKH NSSKENLTN SIASNETPP LYSKPTKIL CEVKFPYKA QNDDELTLK EGDLVVLIS KDGQDPGWW KGELNGVVG VFPDNFVTV LPSAGDETP QKDERRVKS LVEPHSIKH GTVSAQRKS LEVHNDKSE KLENENLSH TKTTPPLPG KKPTIPLKK SPSGSSSSG GIFQGLKKK LVDVVDGAT GSKISPPKP EPCESALPE NVFDDVHRR PLLSDVRAS RPKAPGRRP PSIIGKDQE PGLMNGNSD HNALETMSE AAFSVSSSS EGDPTESKP KLPEWAKHP APWLEEMKL NQAKRSSFT PPQDKLRLT PTDKTDDEA KSKSFKKDP SPVDKSKSI ANARVKTPT NEFPAIRNK PNVPIPSKP QTIADVFTP QKPITKVSP ASNKTPSPM HRIDTNLDS SAGGDSGEE LTSKQYAEL EHRIARLEE LFEKQRQNY EAAIEELRG KLQIETERR MALEANFDK FLQDGAIQQ L
- >TC034557 RA
atggaagtg ttagtagaa cacgactat attgcgaaa gaacccaac gaattaaca atcacaagg ggcgatatc atcaaggat gtgaccaaa aagcagggt ggttggtgg gagggcacg ttgaaggac aagaagggc ttgttcccg gataatttt gttaaagtg ttagacaaa gactcctct gtagtgcta aggaatagg aaggacgcc tccaggata aggcagtgt agggttgtg tttagctat aagcaagac catgaagac gagcttaat ttaaacgtg ggtgatatc attgatata ttgggggaa gaggaggag ggctggtgg agaggagtc ctcaacggg aaggagggc gtcttcccc tcaaacttt gttgaggag attgcgccc cttgcctcc aaacacaac agtagcaag gagaactta accaacagc attgccagc aatgaaacg ccgcccctt tattccaaa ccaaccaag attttgtgc gaagtcaaa tttccctac aaggcacaa aacgacgac gagctgacg ctcaaagaa ggcgatctc gtggtcctt atcagcaaa gacggccag gaccccggc tggtggaaa ggcgagctg aacggcgtt gttggggtt ttccccgac aatttcgtc acagttttg ccttcagcc ggcgatgaa acgccccaa aaagacgag agaagagtc aagtccctc gtggagcca cattccatt aaacacggc acagtcagt gcccaacga aagtcctta gaagtgcat aatgataag agtgaaaaa ctcgaaaat gagaatttg tcacacact aaaaccacg ccaccccta ccgggcaaa aagcccaca attccgctt aaaaaatca ccatcgggg tcgagttcc tccggtggt atttttcaa gggctgaaa aagaaacta gttgatgtg gtggatgga gccactggg agcaaaatt tccccgcct aaacccgaa ccttgtgaa agtgctttg cccgaaaat gtgttcgat gatgtgcac aggaggccg ctgctttcg gacgtgagg gcaagcagg cccaaagcc ccagggcgg cggccgcct tccatcatc ggcaaagac caagagccg ggtctcatg aacgggaac agcgaccac aacgcgctg gaaaccatg tctgaggca gcgttcagc gtgagttcc agctcggag ggcgacccg accgaaagc aagcccaag ctgcccgag tgggcaaaa catccggct ccatggctg gaagaaatg aaactcaat caggcaaaa cgcagctcg tttacgcca ccgcaagac aagctcaga ttaacccct acggacaag accgacgac gaggccaag tcgaagtcg ttcaaaaag gatcccagt cccgtcgac aaatctaag tcaatagcg aatgcaaga gtaaagaca cccaccaac gaatttccc gccattcga aataaacct aacgtgcca atacctagc aagccacag acaatagct gatgtgttt acgccacaa aagccgatc acgaaagtg tcacctgcc agtaataaa actccctcg cctatgcat agaatcgac acgaatctt gacagcagc gctggagga gattccggt gaagaactg acgtcgaaa caatacgct gaattggag catcggata gccagactt gaagagttg ttcgaaaag caacggcag aattatgaa gcggccatt gaggaactt agagggaaa ctgcaaatt gaaacggag aggaggatg gctcttgaa gcgaatttc gacaagttt ctacaagac ggtgcaatt caacaactt taa
- >TC034557 RB
atggcagaa gtgttagta gaacacgac tatattgcg aaagaaccc aacgaatta acaatcaca aggggcgat atcatcaag gatgtgacc aaaaagcag ggtggttgg tgggagggc acgttgaag gacaagaag ggcttgttc ccggataat tttgttaaa gtgttagac aaagactcc tctgtagtg ctaaggaat aggaaggac gcctccagg ataaggcag tgtagggtt gtgtttagc tataagcaa gaccatgaa gacgagctt aatttaaac gtgggtgat atcattgat atattgggg gaagaggag gagggctgg tggagagga gtcctcaac gggaaggag ggcgtcttc ccctcaaac tttgttgag gagattgcg ccccttgcc tccaaacac aacagtagc aaggagaac ttaaccaac agcattgcc agcaatgaa acgccgccc ctttattcc aaaccaacc aagattttg tgcgaagtc aaatttccc tacaaggca caaaacgac gacgagctg acgctcaaa gaaggcgat ctcgtggtc cttatcagc aaagacggc caggacccc ggctggtgg aaaggcgag ctgaacggc gttgttggg gttttcccc gacaatttc gtcacagtt ttgccttca gccggcgat gaaacgccc caaaaagac gagagaaga gtcaagtcc ctcgtggag ccacattcc attaaacac ggcacagtc agtgcccaa cgaaagtcc ttagaagtg cataatgat aagagtgaa aaactcgaa aatgagaat ttgtcacac actaaaacc acgccaccc ctaccgggc aaaaagccc acaattccg cttaaaaaa tcaccatcg gggtcgagt tcctccggt ggtattttt caagggctg aaaaagaaa ctagttgat gtggtggat ggagccact gggagcaaa atttccccg cctaaaccc gaaccttgt gaaagtgct ttgcccgaa aatgtgttc gatgatgtg cacaggagg ccgctgctt tcggacgtg agggcaagc aggcccaaa gccccaggg cggcggccg ccttccatc atcggcaaa gaccaagag ccgggtctc atgaacggg aacagcgac cacaacgcg ctggaaacc atgtctgag gcagcgttc agcgtgagt tccagctcg gagggcgac ccgaccgaa agcaagccc aagctgccc gagtgggca aaacatccg gctccatgg ctggaagaa atgaaactc aatcaggca aaacgcagc tcgtttacg ccaccgcaa gacaagctc agattaacc cctacggac aagaccgac gacgaggcc aagtcgaag tcgttcaaa aaggatccc agtcccgtc gacaaatct aagtcaata gcgaatgca agagtaaag acacccacc aacgaattt cccgccatt cgaaataaa cctaacgtg ccaatacct agcaagcca cagacaata gctgatgtg tttacgcca caaaagccg atcacgaaa gtgtcacct gccagtaat aaaactccc tcgcctatg catagaatc gacacgaat cttgacagc agcgctgga ggagattcc ggtgaagaa ctgacgtcg aaacaatac gctgaattg gagcatcgg atagccaga cttgaagag ttgttcgaa aagcaacgg cagaattat gaagcggcc attgaggaa cttagaggg aaactgcaa attgaaacg gagaggagg atggctctt gaagcgaat ttcgacaag tttctacaa gacggtgca attcaacaa ctttaa
- >TC034557 RA
gtcagtgac atttgtagg gaattttag tgtcaggca aaacagtaa acgtagttg cttaaagtt aataaatat tagaatatt atttattat gatatcata agggtaggg caagtttga ttatgtctt acaacaaca catgaaaaa tcctgcgca tggtcacaa tggaagtgt tagtagaac acgactata ttgcgaaag aacccaacg aattaacaa tcacaaggg gcgatatca tcaaggatg tgaccaaaa agcagggtg gttggtggg agggcacgt tgaaggaca agaagggct tgttcccgg ataattttg ttaaagtgt tagacaaag actcctctg tagtgctaa ggaatagga aggacgcct ccaggataa ggcagtgta gggttgtgt ttagctata agcaagacc atgaagacg agcttaatt taaacgtgg gtgatatca ttgatatat tgggggaag aggaggagg gctggtgga gaggagtcc tcaacggga aggagggcg tcttcccct caaactttg ttgaggaga ttgcgcccc ttgcctcca aacacaaca gtagcaagg agaacttaa ccaacagca ttgccagca atgaaacgc cgccccttt attccaaac caaccaaga ttttgtgcg aagtcaaat ttccctaca aggcacaaa acgacgacg agctgacgc tcaaagaag gcgatctcg tggtcctta tcagcaaag acggccagg accccggct ggtggaaag gcgagctga acggcgttg ttggggttt tccccgaca atttcgtca cagttttgc cttcagccg gcgatgaaa cgccccaaa aagacgaga gaagagtca agtccctcg tggagccac attccatta aacacggca cagtcagtg cccaacgaa agtccttag aagtgcata atgataaga gtgaaaaac tcgaaaatg agaatttgt cacacacta aaaccacgc cacccctac cgggcaaaa agcccacaa ttccgctta aaaaatcac catcggggt cgagttcct ccggtggta tttttcaag ggctgaaaa agaaactag ttgatgtgg tggatggag ccactggga gcaaaattt ccccgccta aacccgaac cttgtgaaa gtgctttgc ccgaaaatg tgttcgatg atgtgcaca ggaggccgc tgctttcgg acgtgaggg caagcaggc ccaaagccc cagggcggc ggccgcctt ccatcatcg gcaaagacc aagagccgg gtctcatga acgggaaca gcgaccaca acgcgctgg aaaccatgt ctgaggcag cgttcagcg tgagttcca gctcggagg gcgacccga ccgaaagca agcccaagc tgcccgagt gggcaaaac atccggctc catggctgg aagaaatga aactcaatc aggcaaaac gcagctcgt ttacgccac cgcaagaca agctcagat taaccccta cggacaaga ccgacgacg aggccaagt cgaagtcgt tcaaaaagg atcccagtc ccgtcgaca aatctaagt caatagcga atgcaagag taaagacac ccaccaacg aatttcccg ccattcgaa ataaaccta acgtgccaa tacctagca agccacaga caatagctg atgtgttta cgccacaaa agccgatca cgaaagtgt cacctgcca gtaataaaa ctccctcgc ctatgcata gaatcgaca cgaatcttg acagcagcg ctggaggag attccggtg aagaactga cgtcgaaac aatacgctg aattggagc atcggatag ccagacttg aagagttgt tcgaaaagc aacggcaga attatgaag cggccattg aggaactta gagggaaac tgcaaattg aaacggaga ggaggatgg ctcttgaag cgaatttcg acaagtttc tacaagacg gtgcaattc aacaacttt aaaccctta tttttatat tatttatag ctaagtatt ttttcgcca tttatatta gactgtagg tgatgcagc atagcgttt tgaccccga aactcagcg atttaattc cactcgaaa cgcttttta tagaataaa agtttaata cagtattac ttttggcac taaaaatac tgcctattt gaaactaac ccgtttttt agttatatt gtctatggt aaaaattgc aattaccat gctttatag agaggtaga ggaaaaaag ttcatataa taattttac aacgtcttt ctgattatt tatttgtga ccaagcaca cacttttgc ttttttcga atagttcct actttaaac aacgactaa cttattgaa gttattcac ccaaaaaat taagcgtga ataatttag tttttctgg aattggaaa cgttttctc catatcttt gtctagtca ttaaagtaa cgtgttacc caattagtc ttccctgag tttttcagt gacattttt cataattgg tccaactag cttcatttt tagtacaac tatcgatta aattgctga gtttgtgca aaatcatga gagagaatt gcaagattt tatataaat gaattagct accgcagtt tttatatag cgtttttac cacattagc ctaattaaa ttaacaggt acatgatag ttgtatgta agtattaat gaaaatcat tccattgct cagagtgaa aatttagaa ttttcactg tatttattt atccttttg ttcatcgat tccattacg tgtgtatat tttaacctt tttatatgt tacgatttt tagcatctt gtagcggct ttcgtcaaa aatatgttt gccacaatt gtaaattat ttattggcg ccacacgtc atatcagtt atttttggc gaactggcc gaatgacat acataacta ttttgttag tgagtcttg tatttttgt attcttgta taattatta tgaataagt gttcaaaga cggtgatag gataatttt aaagtaact taatgaatc gctaagtgg aattgcttt aatcctggt agctcaaga cttttagct catattttg ctacttcag tagattgtg caagaacta tattttgaa gaggtaaga gatatcttg taataatgt aacattcca taattcact taatgtaaa taaaataaa aaatgaaaa attgt
- >TC034557 RB
gtcagtgac atttgtagg gaattttag tgtcaggca aaacagtaa acgtagttg cttaaagtt aataaatat tagaatatt atttattat gatatcata agggtaggg caagtttga ttatgtctt acaacaaca catgaaaaa tcctgcgca tggtcacaa tggcagaag tgttagtag aacacgact atattgcga aagaaccca acgaattaa caatcacaa ggggcgata tcatcaagg atgtgacca aaaagcagg gtggttggt gggagggca cgttgaagg acaagaagg gcttgttcc cggataatt ttgttaaag tgttagaca aagactcct ctgtagtgc taaggaata ggaaggacg cctccagga taaggcagt gtagggttg tgtttagct ataagcaag accatgaag acgagctta atttaaacg tgggtgata tcattgata tattggggg aagaggagg agggctggt ggagaggag tcctcaacg ggaaggagg gcgtcttcc cctcaaact ttgttgagg agattgcgc cccttgcct ccaaacaca acagtagca aggagaact taaccaaca gcattgcca gcaatgaaa cgccgcccc tttattcca aaccaacca agattttgt gcgaagtca aatttccct acaaggcac aaaacgacg acgagctga cgctcaaag aaggcgatc tcgtggtcc ttatcagca aagacggcc aggaccccg gctggtgga aaggcgagc tgaacggcg ttgttgggg ttttccccg acaatttcg tcacagttt tgccttcag ccggcgatg aaacgcccc aaaaagacg agagaagag tcaagtccc tcgtggagc cacattcca ttaaacacg gcacagtca gtgcccaac gaaagtcct tagaagtgc ataatgata agagtgaaa aactcgaaa atgagaatt tgtcacaca ctaaaacca cgccacccc taccgggca aaaagccca caattccgc ttaaaaaat caccatcgg ggtcgagtt cctccggtg gtatttttc aagggctga aaaagaaac tagttgatg tggtggatg gagccactg ggagcaaaa tttccccgc ctaaacccg aaccttgtg aaagtgctt tgcccgaaa atgtgttcg atgatgtgc acaggaggc cgctgcttt cggacgtga gggcaagca ggcccaaag ccccagggc ggcggccgc cttccatca tcggcaaag accaagagc cgggtctca tgaacggga acagcgacc acaacgcgc tggaaacca tgtctgagg cagcgttca gcgtgagtt ccagctcgg agggcgacc cgaccgaaa gcaagccca agctgcccg agtgggcaa aacatccgg ctccatggc tggaagaaa tgaaactca atcaggcaa aacgcagct cgtttacgc caccgcaag acaagctca gattaaccc ctacggaca agaccgacg acgaggcca agtcgaagt cgttcaaaa aggatccca gtcccgtcg acaaatcta agtcaatag cgaatgcaa gagtaaaga cacccacca acgaatttc ccgccattc gaaataaac ctaacgtgc caataccta gcaagccac agacaatag ctgatgtgt ttacgccac aaaagccga tcacgaaag tgtcacctg ccagtaata aaactccct cgcctatgc atagaatcg acacgaatc ttgacagca gcgctggag gagattccg gtgaagaac tgacgtcga aacaatacg ctgaattgg agcatcgga tagccagac ttgaagagt tgttcgaaa agcaacggc agaattatg aagcggcca ttgaggaac ttagaggga aactgcaaa ttgaaacgg agaggagga tggctcttg aagcgaatt tcgacaagt ttctacaag acggtgcaa ttcaacaac tttaaaccc ttattttta tattattta tagctaagt attttttcg ccatttata ttagactgt aggtgatgc agcatagcg ttttgaccc cgaaactca gcgatttaa ttccactcg aaacgcttt ttatagaat aaaagttta atacagtat tacttttgg cactaaaaa tactgccta tttgaaact aacccgttt tttagttat attgtctat ggtaaaaat tgcaattac catgcttta tagagaggt agaggaaaa aagttcata taataattt tacaacgtc tttctgatt atttatttg tgaccaagc acacacttt tgctttttt cgaatagtt cctacttta aacaacgac taacttatt gaagttatt cacccaaaa aattaagcg tgaataatt tagtttttc tggaattgg aaacgtttt ctccatatc tttgtctag tcattaaag taacgtgtt acccaatta gtcttccct gagtttttc agtgacatt tttcataat tggtccaac tagcttcat ttttagtac aactatcga ttaaattgc tgagtttgt gcaaaatca tgagagaga attgcaaga ttttatata aatgaatta gctaccgca gtttttata tagcgtttt taccacatt agcctaatt aaattaaca ggtacatga tagttgtat gtaagtatt aatgaaaat cattccatt gctcagagt gaaaattta gaattttca ctgtattta tttatcctt ttgttcatc gattccatt acgtgtgta tattttaac ctttttata tgttacgat ttttagcat cttgtagcg gctttcgtc aaaaatatg tttgccaca attgtaaat tatttattg gcgccacac gtcatatca gttattttt ggcgaactg gccgaatga catacataa ctattttgt tagtgagtc ttgtatttt tgtattctt gtataatta ttatgaata agtgttcaa agacggtga taggataat tttaaagta acttaatga atcgctaag tggaattgc tttaatcct ggtagctca agactttta gctcatatt ttgctactt cagtagatt gtgcaagaa ctatatttt gaagaggta agagatatc ttgtaataa tgtaacatt ccataattc acttaatgt aaataaaat aaaaaatga aaaattgt
