Status
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Gene information
General information
TC001051 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 10207381..10209696
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001051
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00209

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001051 RA
      MSIEIESAD VIRLIQQYL KESNLLRTL HTLQEETGV 
      TLNTVDSVD GFCADINNG HWDTVLKAI QSLKLPDKK 
      LIDLYEQIV LELIELREL GAARSLLRQ TDPMIMMKQ 
      NEPERYIHL ENLLARSYF DPREAYPDG GSKEKRRAA 
      IAHALSGEV SVVPSSRLL ALLGQALKW QQHQGLLPP 
      GTTIDLFRG KAAIRDQED EQFPTQMAK QIKFGQKSH 
      VECARFSPD GQYLVTGSI DGIIEVWNF TTGKIRKDL 
      KYQAQESFM MMEHAVLCM AFSRDSEML VTGSQTGRI 
      KVWRISTGQ CLRKIEKAH SKGITCLQF SRDNSQVLS 
      ASFDHTIRL HGLKSGKIL KEFRGHTSF VNEVIFTQD 
      GHNVLSASS DGTVKVWSI RAAECINTF KSLGAGDIT 
      VNNIHNFAK NPEHFVVCN RTNTVVIMN LQGQIVRSF 
      ASGKREGGD FVCSTVSPR GDWIYCVGE DLVLYCFST 
      NSGKLERTL NIHEKDVIG IAHHPHQNL LCTYSEDGL 
      VRLWKP
    CDS sequence
    • >TC001051 RA
      atgtctata gaaatcgaa tctgcggac gtaatccgc 
      ctaattcag caatattta aaagaatct aacttatta 
      agaactctt cacacacta caggaggaa actggtgta 
      acattaaac actgtagat agtgttgat gggttttgt 
      gccgacata aacaacggg cattgggac actgttttg 
      aaagcgata cagtccctt aaactccct gataaaaaa 
      ttaatcgac ttgtacgaa cagattgtt ttagaatta 
      attgaactc agagagtta ggagcagcc agatcccta 
      ctcagacaa acagacccc atgataatg atgaagcaa 
      aacgagccc gagcgttac attcaccta gaaaattta 
      ctcgctcgt tcatacttt gacccccgt gaagcttat 
      ccagacgga ggcagcaaa gaaaaacgc cgtgctgct 
      attgctcac gccctttca ggggaagta tcagttgta 
      ccatcgtca cgtcttttg gccttactt ggccaggcg 
      ctaaaatgg caacagcac caaggcttg ttgcctcct 
      ggtaccaca attgacttg tttaggggc aaagctgcc 
      atcagagac caggaagac gaacaattc ccaactcaa 
      atggccaaa caaatcaag tttgggcaa aagtcgcac 
      gtggagtgc gctcgcttt tcaccagat ggacaatat 
      ctggtcaca gggagcatt gatgggatt attgaggtg 
      tggaatttc accaccggg aaaattcgc aaggacttg 
      aaatatcaa gcccaggag agctttatg atgatggaa 
      catgcagta ttatgtatg gcgttttcg cgagacagc 
      gaaatgttg gtaactggg tcacagacc ggaagaatc 
      aaagtttgg aggattagc acgggccag tgtttgcga 
      aaaatcgaa aaagctcac tcgaaaggt atcacctgt 
      ttacagttt tctagagac aacagtcag gttctaagc 
      gcttcgttt gatcacact attagatta catggccta 
      aaatctggg aaaatattg aaggagttt cgtgggcac 
      acttcgttc gtaaatgaa gttattttc actcaggat 
      ggtcataac gttttgagt gcttcttct gacggaacc 
      gttaaagtg tggagcatc cgagctgcg gaatgcatc 
      aatactttc aaatcttta ggcgcgggc gatatcacg 
      gtgaataat attcacaac tttgcaaaa aatcccgaa 
      cattttgtt gtctgtaat cgcacgaat actgtagtt 
      attatgaat cttcaagga cagattgtg aggtcattc 
      gcaagtggt aaacgtgaa ggtggtgat tttgtatgt 
      tcaacagtg tcaccaaga ggtgactgg atttattgc 
      gtcggggaa gatctagtc ttgtattgc ttttccaca 
      aattctggt aaactcgag agaacttta aatattcat 
      gaaaaagac gttattggt atagcacac catccacat 
      cagaattta ctctgtaca tatagcgag gacggactc 
      gtgcgtttg tggaaaccg taa
    mRNA sequence
    • >TC001051 RA
      aatgtcaaa aacgtaagt tggagttgt caaaaaaat 
      aggatcatt ttatttgca atttagcac ttttttgtc 
      atttgaaac gtgattatt agcacaaga ataataacg 
      taaagcctc tgaagccac aactcctaa aatatgtct 
      atagaaatc gaatctgcg gacgtaatc cgcctaatt 
      cagcaatat ttaaaagaa tctaactta ttaagaact 
      cttcacaca ctacaggag gaaactggt gtaacatta 
      aacactgta gatagtgtt gatgggttt tgtgccgac 
      ataaacaac gggcattgg gacactgtt ttgaaagcg 
      atacagtcc cttaaactc cctgataaa aaattaatc 
      gacttgtac gaacagatt gttttagaa ttaattgaa 
      ctcagagag ttaggagca gccagatcc ctactcaga 
      caaacagac cccatgata atgatgaag caaaacgag 
      cccgagcgt tacattcac ctagaaaat ttactcgct 
      cgttcatac tttgacccc cgtgaagct tatccagac 
      ggaggcagc aaagaaaaa cgccgtgct gctattgct 
      cacgccctt tcaggggaa gtatcagtt gtaccatcg 
      tcacgtctt ttggcctta cttggccag gcgctaaaa 
      tggcaacag caccaaggc ttgttgcct cctggtacc 
      acaattgac ttgtttagg ggcaaagct gccatcaga 
      gaccaggaa gacgaacaa ttcccaact caaatggcc 
      aaacaaatc aagtttggg caaaagtcg cacgtggag 
      tgcgctcgc ttttcacca gatggacaa tatctggtc 
      acagggagc attgatggg attattgag gtgtggaat 
      ttcaccacc gggaaaatt cgcaaggac ttgaaatat 
      caagcccag gagagcttt atgatgatg gaacatgca 
      gtattatgt atggcgttt tcgcgagac agcgaaatg 
      ttggtaact gggtcacag accggaaga atcaaagtt 
      tggaggatt agcacgggc cagtgtttg cgaaaaatc 
      gaaaaagct cactcgaaa ggtatcacc tgtttacag 
      ttttctaga gacaacagt caggttcta agcgcttcg 
      tttgatcac actattaga ttacatggc ctaaaatct 
      gggaaaata ttgaaggag tttcgtggg cacacttcg 
      ttcgtaaat gaagttatt ttcactcag gatggtcat 
      aacgttttg agtgcttct tctgacgga accgttaaa 
      gtgtggagc atccgagct gcggaatgc atcaatact 
      ttcaaatct ttaggcgcg ggcgatatc acggtgaat 
      aatattcac aactttgca aaaaatccc gaacatttt 
      gttgtctgt aatcgcacg aatactgta gttattatg 
      aatcttcaa ggacagatt gtgaggtca ttcgcaagt 
      ggtaaacgt gaaggtggt gattttgta tgttcaaca 
      gtgtcacca agaggtgac tggatttat tgcgtcggg 
      gaagatcta gtcttgtat tgcttttcc acaaattct 
      ggtaaactc gagagaact ttaaatatt catgaaaaa 
      gacgttatt ggtatagca caccatcca catcagaat 
      ttactctgt acatatagc gaggacgga ctcgtgcgt 
      ttgtggaaa ccgtaatta ttgtaattt tgtaacgtt 
      tgtaatcct aagaaataa agcgtaaat tcaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY