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Gene information
General information
TC001032 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 4479287..4482042
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001032
    iBeetle screen iB_00208
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001032 RA
      MGERIEDYE VYNLLGKGG FASVYHAKC LKTGLNVAI 
      KMIDKKMMQ AAGMVKRVE QEVSIHYRL KHPSILELY 
      TFFEDANYV YLVLELCHN GELRQYIKT KALTESEVS 
      SIMKQVVEG MKYLHTHNI LHRDISLSN LLLTKDMQV 
      KIADFGLAT QLSRPDEKH TTMCGTPNF ISPEVLSRT 
      SHGLEADVW GLGCLLYNL LVGSPPFDT HGVKNTLNR 
      IVSANFHLP SHLSPEAKD LINSLLQKN PKDRIKLDQ 
      ILEHPFITR GQLLMSHLT HDSGIHTMS SRRDSAFSE 
      GASNNIYNF KSNSHNMEQ LSRKLHDVN LRSTYSDPP 
      LFSNCGEIR SHCSCELPQ NNAYPADPG GNRILKDNS 
      NYKPIFSTT SNSSSKSDK SKTQRLLQL CSQRLLPKT 
      HRTENSILH IMEDGEVVV ELMKKKGSA KRNVVCDVL 
      RISSDGAKI TIYEPNGGK GVTPGAAPP PIPPQGGYE 
      AYTIENLPE KHWKKYMHA YQFVALIKE KTPKVTCYN 
      DKMKCVLME NLTDFEATF YEGGTVTKS SLKGITFQD 
      SSGNKLLVK TSNECRSLT GTFEYMWSQ SQEALKHCL 
      LLEEMLSKL AGPNFPAII GRRPCSGPG KENQTQTTM 
      MPSFAVSMN STAVGSFQS SSSLKEKKV SVPGVGTAV 
      QLPNGEVQV RYGDGSQLS MDGKHQIKY QYSDGNTVT 
      YSDNDNIPR PIREKLQHM PKILKQLMP SPTTHNFRL
    CDS sequence
    • >TC001032 RA
      atgggtgaa cgaattgag gactacgaa gtttacaat 
      ttgttaggg aaaggaggt tttgcctcc gtatatcac 
      gcaaaatgt ttaaaaacc ggattaaat gtcgctata 
      aaaatgatc gacaagaaa atgatgcaa gcggctggg 
      atggtaaaa cgtgtcgaa caggaagta tcaattcat 
      tacagactt aaacaccct tctattttg gagttgtat 
      acgtttttt gaggacgct aattatgtt tatctcgtt 
      ttggagtta tgtcacaat ggggagctc aggcagtat 
      atcaaaact aaggcatta actgagagt gaagtaagc 
      agtattatg aaacaagtg gtagaaggc atgaaatat 
      ctccacacc cacaatatt ctccacaga gacatttcc 
      ttatcaaac ttattacta accaaagac atgcaagtc 
      aaaattgca gattttggt ttggccaca caattatca 
      cgtcctgat gaaaaacac acaacaatg tgtggaacc 
      ccaaatttt atctcaccc gaagttctt tcacggact 
      tcccacggc cttgaagcc gacgtttgg ggccttggt 
      tgtctcctc tataattta ttagtgggc tcaccacct 
      tttgacact cacggtgtt aaaaatact ctaaatcgc 
      atcgtttcg gccaatttt catttaccc agtcatttg 
      tcaccagaa gctaaggac ttaattaat tcacttcta 
      cagaaaaat cctaaagat cgcatcaag ttggaccag 
      attttggag caccctttt ataacaaga ggacagttg 
      ttgatgtca catttgact catgatagt gggattcac 
      acaatgtct agccgacgt gatagcgct tttagcgaa 
      ggtgctagt aataatatt tacaatttt aaaagcaac 
      tctcataat atggaacaa ctgtcgcgg aaacttcac 
      gatgttaat ttgaggtcg acctattcg gaccctcca 
      ctgttctca aattgtggc gaaattcgg agccactgc 
      agttgcgaa ctgcctcaa aataacgca tatcccgct 
      gatcctgga gggaacagg atcctcaaa gacaactcg 
      aactacaaa ccaattttt tctacaact agtaacagt 
      tcgagtaaa agtgacaag agtaaaacc cagcgattg 
      ttgcaactg tgttcgcaa agactactt ccgaaaacg 
      caccgaacc gaaaattcc attttacac ataatggaa 
      gacggtgaa gtcgtcgtg gaactgatg aagaaaaaa 
      ggaagtgcg aaacgtaac gtcgtttgt gatgtttta 
      cgcatatct tcagacggg gccaaaatc actatttac 
      gaacctaac ggcggtaaa ggggtcacc ccgggggcg 
      gctccgccc ccaataccc ccacaaggg ggctacgaa 
      gcatacact atcgaaaat ttaccggaa aaacactgg 
      aagaagtac atgcacgcg taccaattc gtggccctt 
      attaaggaa aaaacccca aaggttacg tgttacaat 
      gataagatg aagtgcgtt ctgatggag aatttgacc 
      gattttgag gccactttt tacgaaggt ggaaccgtg 
      acgaaatcg tctttgaaa gggattacg tttcaagac 
      tcgtccggt aacaaactt ttggtcaaa acaagtaac 
      gaatgccgg agtttgacc ggaactttt gagtatatg 
      tggtcgcag tcgcaggag gctttgaaa cactgcctc 
      cttttggag gagatgttg tcaaagttg gctgggccc 
      aattttccc gctattata ggcaggagg ccgtgctcg 
      gggccaggg aaggagaat cagacccag actaccatg 
      atgccctcg tttgctgtt tctatgaac agtacggct 
      gtagggtcg tttcagagc agttcctct ttgaaggag 
      aagaaggtg tcagtgcca ggggtgggc acggccgtg 
      caattgccc aatggagaa gtgcaagta aggtatggt 
      gacggatcc cagttgtca atggacggt aaacaccaa 
      atcaaatat cagtattcg gacgggaat accgttacg 
      tatagtgat aatgataat atcccgcgt ccgataagg 
      gaaaagttg caacacatg ccaaaaatt ttgaaacag 
      ttgatgccc agtccgact actcataat tttcggtta 
      tag
    mRNA sequence
    • >TC001032 RA
      gtttttgaa aaacatttg ttttgtttt cagttattt 
      tcaaatgat tttcggctg aaatcaatc aattttgac 
      ccaaaaatg cattaacaa agtaaaaat tccttcctg 
      agcccattg agaggtgat tttaaatgt ttctcgacg 
      tggtttcac cttcaaact caaatgaat ctcgaaatg 
      ggtgaacga attgaggac tacgaagtt tacaatttg 
      ttagggaaa ggaggtttt gcctccgta tatcacgca 
      aaatgttta aaaaccgga ttaaatgtc gctataaaa 
      atgatcgac aagaaaatg atgcaagcg gctgggatg 
      gtaaaacgt gtcgaacag gaagtatca attcattac 
      agacttaaa cacccttct attttggag ttgtatacg 
      ttttttgag gacgctaat tatgtttat ctcgttttg 
      gagttatgt cacaatggg gagctcagg cagtatatc 
      aaaactaag gcattaact gagagtgaa gtaagcagt 
      attatgaaa caagtggta gaaggcatg aaatatctc 
      cacacccac aatattctc cacagagac atttcctta 
      tcaaactta ttactaacc aaagacatg caagtcaaa 
      attgcagat tttggtttg gccacacaa ttatcacgt 
      cctgatgaa aaacacaca acaatgtgt ggaacccca 
      aattttatc tcacccgaa gttctttca cggacttcc 
      cacggcctt gaagccgac gtttggggc cttggttgt 
      ctcctctat aatttatta gtgggctca ccacctttt 
      gacactcac ggtgttaaa aatactcta aatcgcatc 
      gtttcggcc aattttcat ttacccagt catttgtca 
      ccagaagct aaggactta attaattca cttctacag 
      aaaaatcct aaagatcgc atcaagttg gaccagatt 
      ttggagcac ccttttata acaagagga cagttgttg 
      atgtcacat ttgactcat gatagtggg attcacaca 
      atgtctagc cgacgtgat agcgctttt agcgaaggt 
      gctagtaat aatatttac aattttaaa agcaactct 
      cataatatg gaacaactg tcgcggaaa cttcacgat 
      gttaatttg aggtcgacc tattcggac cctccactg 
      ttctcaaat tgtggcgaa attcggagc cactgcagt 
      tgcgaactg cctcaaaat aacgcatat cccgctgat 
      cctggaggg aacaggatc ctcaaagac aactcgaac 
      tacaaacca attttttct acaactagt aacagttcg 
      agtaaaagt gacaagagt aaaacccag cgattgttg 
      caactgtgt tcgcaaaga ctacttccg aaaacgcac 
      cgaaccgaa aattccatt ttacacata atggaagac 
      ggtgaagtc gtcgtggaa ctgatgaag aaaaaagga 
      agtgcgaaa cgtaacgtc gtttgtgat gttttacgc 
      atatcttca gacggggcc aaaatcact atttacgaa 
      cctaacggc ggtaaaggg gtcaccccg ggggcggct 
      ccgccccca ataccccca caagggggc tacgaagca 
      tacactatc gaaaattta ccggaaaaa cactggaag 
      aagtacatg cacgcgtac caattcgtg gcccttatt 
      aaggaaaaa accccaaag gttacgtgt tacaatgat 
      aagatgaag tgcgttctg atggagaat ttgaccgat 
      tttgaggcc actttttac gaaggtgga accgtgacg 
      aaatcgtct ttgaaaggg attacgttt caagactcg 
      tccggtaac aaacttttg gtcaaaaca agtaacgaa 
      tgccggagt ttgaccgga acttttgag tatatgtgg 
      tcgcagtcg caggaggct ttgaaacac tgcctcctt 
      ttggaggag atgttgtca aagttggct gggcccaat 
      tttcccgct attataggc aggaggccg tgctcgggg 
      ccagggaag gagaatcag acccagact accatgatg 
      ccctcgttt gctgtttct atgaacagt acggctgta 
      gggtcgttt cagagcagt tcctctttg aaggagaag 
      aaggtgtca gtgccaggg gtgggcacg gccgtgcaa 
      ttgcccaat ggagaagtg caagtaagg tatggtgac 
      ggatcccag ttgtcaatg gacggtaaa caccaaatc 
      aaatatcag tattcggac gggaatacc gttacgtat 
      agtgataat gataatatc ccgcgtccg ataagggaa 
      aagttgcaa cacatgcca aaaattttg aaacagttg 
      atgcccagt ccgactact cataatttt cggttatag 
      ttttactat tgttcattg aaaatgaaa tgcgttttg 
      taacgagct gaccagttt ttcattgca tagagtaag 
      atttttaaa aattgaata aatattttt gcactatta 
      attgatgac actgttttt atttgtgac aataaagat 
      tgaacttta tg