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Gene information
General information
TC001020 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 4399907..4403758
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001020
    iBeetle screen iB_00207
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 11.11% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001020 RA
      MGVKDLFTW YQKKIGTYD KQKWETTIE QKILYGLTH 
      VQMKSTKPN TDFIDLDLV RGSSFSKIK PKQGFMTVA 
      KLATFRCLF LPIYSKWWM QQTSVSIFT LMFCLYVSQ 
      LTNIAIYYK NSWTNQMLD KSPENVMCM ELIVPFLMT 
      WVLSLVHSQ IAATMPVTD SQKTKLTKS RNFRRRLSA 
      VRKIPTDGS SAESLLNLR IHKKNKRIH FNLNSVSEL 
      NCKEINKNL RKRRSSDGV PADDDGFES LNGNGSRTS 
      EEEIVPTAC KNALKRCDE WVKKQSLSN ISTESRHST 
      EPESDVDES DVKRAKYKI PTINITSMD STCDTDDEY 
      EKTSIKTSK MSSSTNEWM NVTTNSEDC SYSSDLDEV 
      SDGHIETNH DDYDHDLTS TAVFNPPSI KVSCIIWKR 
      NEIKKADLS MLDISSAII GKVESTSQS MDYFYLGII 
      VSTIHAFIP ILFRVCNST LESNSFVDG QYSIFELFD 
      SIMGKLPNS VPTLIEAAF GTTLWERAI IITACLQRW 
      FLASLFFFL LAVAENTFK QRFLYAKLF SHLTSFRRA 
      QQSELPHFR LNKVRNIKT WLSVRSYLK QRGPQRSVD 
      VIVSTAFII TLLLLSFLC VELLKETGA LNSQYHVEA 
      LIWCLGVGI LLLRFMTLG NKINKKYRN LSVLITEQI 
      NLHLQIEQK PQKKEELAL ANNVLKLAI DLIKELQSP 
      FKISGLSAN SILYNTTKL IILSALSGI LSELLGFKL 
      KLHKIKIK
    CDS sequence
    • >TC001020 RA
      atgggggtt aaggatctt tttacatgg tatcagaaa 
      aaaatcggt acttatgac aagcagaaa tgggaaacg 
      actatagaa cagaaaatc ctctacgga ctaacgcac 
      gtccagatg aagagcact aaacccaat acagatttt 
      attgacctt gatttggtt cgaggttcg tccttttcg 
      aaaatcaaa ccaaagcaa ggttttatg accgttgct 
      aaactagca acgtttcgg tgcttattt ctcccgatt 
      tattccaaa tggtggatg caacaaaca tcagtatca 
      atttttacg cttatgttt tgtttatac gtttctcaa 
      ctaacaaac atcgcaata tactacaaa aattcatgg 
      acaaaccaa atgttagat aagtcacct gaaaatgtt 
      atgtgcatg gaattgatc gttccattc ctaatgact 
      tgggtttta agcctcgta cattctcaa atagccgca 
      acaatgcca gtaaccgac tcgcaaaaa accaagcta 
      acaaaatcg cgaaatttc agacgtaga ttgagcgca 
      gttagaaaa ataccaact gacggctcc tcggccgaa 
      tctctccta aacctgcga attcataaa aaaaacaag 
      cgaattcat tttaatctg aacagcgtt tccgaactg 
      aactgcaag gaaatcaac aaaaatctc aggaagcgc 
      cgatctagt gatggagta ccagccgac gacgacggc 
      ttcgagagt ctcaacggt aacggctcg aggacaagt 
      gaggaagaa atagtacca actgcgtgc aaaaatgcc 
      ctaaaacgt tgcgacgaa tgggttaaa aagcaatcc 
      ctttcgaac atttccacc gaatcgaga cacagtact 
      gagcccgaa agcgacgtc gatgagtcc gacgttaaa 
      agagcgaaa tataaaata cccacaatt aacataact 
      tcaatggac agcacgtgt gacactgac gacgaatat 
      gagaaaact tcaatcaaa accagcaaa atgagctcc 
      tcaaccaac gaatggatg aatgttacg accaacagc 
      gaggactgt agctacagc tccgacctg gatgaggtc 
      tccgacggt cacattgaa acaaatcat gacgattat 
      gatcacgat ttgacctcg acggccgtt ttcaaccca 
      ccctcaatc aaagtcagt tgcatcatt tggaagcga 
      aacgaaatc aaaaaagcc gatctttcc atgctggat 
      atcagctcg gccatcatc ggcaaagtc gagtcaaca 
      tcacaaagt atggactat ttctacctc ggaatcatt 
      gtttcgacc attcacgct tttatcccg attttattc 
      cgagtgtgt aactccacc ctcgagtca aattcgttc 
      gtcgatgga caatattcc attttcgaa ttgtttgac 
      tcgatcatg ggtaaatta cccaactcg gtgccgacc 
      ttaatcgag gcggctttc ggaactact ttatgggaa 
      cgggcaatt ataataacg gcgtgtctg caacgctgg 
      ttcctggca agtctcttc tttttcctc ctggccgtt 
      gccgaaaac acattcaaa cagcgcttt ctctacgcg 
      aagctcttc tctcatttg acgtcattc cgacgtgcc 
      cagcagtcg gaattgccc cattttcga ctgaataaa 
      gtgcgaaat ataaaaacg tggctttcg gtgcgctcg 
      tatttgaaa caaagaggg ccccaaaga tccgtcgat 
      gttattgtt tcgacagct tttatcatc actttgctg 
      cttttgtcg tttctctgt gtcgagttg ttgaaggaa 
      actggtgct ttgaattcg caatatcac gtcgaggct 
      ttgatttgg tgtttggga gtggggatt ctgctgctt 
      aggtttatg actttgggg aataaaatt aacaagaag 
      tataggaat ctgtcggtt ttgatcacg gaacagatc 
      aatttgcat ctgcaaatt gagcagaaa ccgcaaaaa 
      aaggaggag ctggcgttg gctaataat gtgctgaag 
      ttggccatc gatctcatt aaagagctg caaagtccg 
      ttcaaaatt tcaggcttg tcggcaaat tccatctta 
      tacaacacc acaaaactg ataattttg tcggcactt 
      tcggggatc ttatcagag ctgttggga tttaagctg 
      aagttgcat aaaatcaaa attaagtaa
    mRNA sequence
    • >TC001020 RA
      tttggcaag caaatatcg ggaaatgtc aaaatcgtc 
      aaagtcaaa atcatatgt gattgtgtt tggtatctg 
      tcacattca aattctcgc tatgtgaac aaattattt 
      acaaaaaga aatcaaaaa aacgtgcaa aaaattcac 
      tgtaaattc accggaagt gtcgggttt cgttaaatt 
      cctttgtgc aaagttcct attccagag cggtttgaa 
      gttaaatgg gggttaagg atcttttta catggtatc 
      agaaaaaaa tcggtactt atgacaagc agaaatggg 
      aaacgacta tagaacaga aaatcctct acggactaa 
      cgcacgtcc agatgaaga gcactaaac ccaatacag 
      attttattg accttgatt tggttcgag gttcgtcct 
      tttcgaaaa tcaaaccaa agcaaggtt ttatgaccg 
      ttgctaaac tagcaacgt ttcggtgct tatttctcc 
      cgatttatt ccaaatggt ggatgcaac aaacatcag 
      tatcaattt ttacgctta tgttttgtt tatacgttt 
      ctcaactaa caaacatcg caatatact acaaaaatt 
      catggacaa accaaatgt tagataagt cacctgaaa 
      atgttatgt gcatggaat tgatcgttc cattcctaa 
      tgacttggg ttttaagcc tcgtacatt ctcaaatag 
      ccgcaacaa tgccagtaa ccgactcgc aaaaaacca 
      agctaacaa aatcgcgaa atttcagac gtagattga 
      gcgcagtta gaaaaatac caactgacg gctcctcgg 
      ccgaatctc tcctaaacc tgcgaattc ataaaaaaa 
      acaagcgaa ttcatttta atctgaaca gcgtttccg 
      aactgaact gcaaggaaa tcaacaaaa atctcagga 
      agcgccgat ctagtgatg gagtaccag ccgacgacg 
      acggcttcg agagtctca acggtaacg gctcgagga 
      caagtgagg aagaaatag taccaactg cgtgcaaaa 
      atgccctaa aacgttgcg acgaatggg ttaaaaagc 
      aatcccttt cgaacattt ccaccgaat cgagacaca 
      gtactgagc ccgaaagcg acgtcgatg agtccgacg 
      ttaaaagag cgaaatata aaataccca caattaaca 
      taacttcaa tggacagca cgtgtgaca ctgacgacg 
      aatatgaga aaacttcaa tcaaaacca gcaaaatga 
      gctcctcaa ccaacgaat ggatgaatg ttacgacca 
      acagcgagg actgtagct acagctccg acctggatg 
      aggtctccg acggtcaca ttgaaacaa atcatgacg 
      attatgatc acgatttga cctcgacgg ccgttttca 
      acccaccct caatcaaag tcagttgca tcatttgga 
      agcgaaacg aaatcaaaa aagccgatc tttccatgc 
      tggatatca gctcggcca tcatcggca aagtcgagt 
      caacatcac aaagtatgg actatttct acctcggaa 
      tcattgttt cgaccattc acgctttta tcccgattt 
      tattccgag tgtgtaact ccaccctcg agtcaaatt 
      cgttcgtcg atggacaat attccattt tcgaattgt 
      ttgactcga tcatgggta aattaccca actcggtgc 
      cgaccttaa tcgaggcgg ctttcggaa ctactttat 
      gggaacggg caattataa taacggcgt gtctgcaac 
      gctggttcc tggcaagtc tcttctttt tcctcctgg 
      ccgttgccg aaaacacat tcaaacagc gctttctct 
      acgcgaagc tcttctctc atttgacgt cattccgac 
      gtgcccagc agtcggaat tgccccatt ttcgactga 
      ataaagtgc gaaatataa aaacgtggc tttcggtgc 
      gctcgtatt tgaaacaaa gagggcccc aaagatccg 
      tcgatgtta ttgtttcga cagctttta tcatcactt 
      tgctgcttt tgtcgtttc tctgtgtcg agttgttga 
      aggaaactg gtgctttga attcgcaat atcacgtcg 
      aggctttga tttggtgtt tgggagtgg ggattctgc 
      tgcttaggt ttatgactt tggggaata aaattaaca 
      agaagtata ggaatctgt cggttttga tcacggaac 
      agatcaatt tgcatctgc aaattgagc agaaaccgc 
      aaaaaaagg aggagctgg cgttggcta ataatgtgc 
      tgaagttgg ccatcgatc tcattaaag agctgcaaa 
      gtccgttca aaatttcag gcttgtcgg caaattcca 
      tcttataca acaccacaa aactgataa ttttgtcgg 
      cactttcgg ggatcttat cagagctgt tgggattta 
      agctgaagt tgcataaaa tcaaaatta agtaagatt 
      ttagtcata cattgttgt ggtttaaaa tgtttatat 
      ttgtttaca ccatttttc aactgtata ttttttgta 
      agtgcaaga aatgactgt tttttgtct agtgttaga 
      caactagta aagggtaac cctgcatct attttactt 
      ctacatcgt gaaatctcc tatttttat gctttttat 
      ttagtcttt aataaatta tcttctgtg ataatccat 
      tttgagcag ctattttta cgtttgaag tatcaagct 
      ttcaaacaa tttcgggtc aaagtgccc tagggacga 
      agtaagttg gcaactccg tgccaattg ttagttaat 
      gttgccaac ttaaattca gccaagggc cactttaat 
      cgaaattcg aaagcagtt gcaattgta gttagtatg 
      tgttatcaa acgtggcaa accttcccc agtgaatgt 
      ttcaatgtt ttaccagca atcaaaaac cttggtaga 
      atgcatgac ggtgtaaat acgtactta atataaaaa 
      atgatctcc atatacaaa aaattgtat ttttataca 
      tgagattca tatttttat gaaatataa agtctggtt 
      aatctccgc ttataaact tcgttttat ttttacaaa 
      acgcattgt atcaaggtt aaaagtagg cgcttagca 
      tgcaaacaa aagctcaat aagaacagg gttttgttt 
      cttgttaga gtaagaaat caattgtaa ctagaacac 
      actttattg taaagtata taaatgtta ataaacatg 
      aagagaaaa a