Status
Please wait ...
Gene information
General information
TC030995
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001007,TC001006
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 4198973..4237939
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030995
    iBeetle screen iB_00206
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 11.11% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 11.11% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030995 RA
      MKIWLCAKT LRKVSQDCL RRVDAWETL LGGFITQLT 
      AALVGVSES SKSRFVESV KPIVQRPYF DDVGPRNVT 
      AVVGQSALL NCRVKHPGD RRVSWMRKR DLHILTSDI 
      HTYTGDARF SVRHPEHSD DWDLRIAYV QKRDAGVYE 
      CQVNTEPKI NLAITLNVE DAQATISGP PEVYVKKGS 
      TISLTCSVN VHSTPPSSV LWYHGASVV DFDSPRGGI 
      SLETEKTEA GTTSKLLVT KALLSDSGN YTCMPSNAS 
      PASAVVHVL NGEHPAAMQ TSRAGVRRQ MALLATFSL 
      LVVTSVR
    • >TC030995 RB
      MKIWLCAKT LRKVSQDCL RRVDAWETL LGGFITQLT 
      AALVGVSES SKSRFVESV KPIVQRPYF DDVGPRNVT 
      AVVGQSALL NCRVKHPGD RRVSWMRKR DLHILTSDI 
      HTYTGDARF SVRHPEHSD DWDLRIAYV QKRDAGVYE 
      CQVNTEPKI NLAITLNVE DASSLAATH PDPRSKDAQ 
      ATISGPPEV YVKKGSTIS LTCSVNVHS TPPSSVLWY 
      HGASVVDFD SPRGGISLE TEKTEAGTT SKLLVTKAL 
      LSDSGNYTC MPSNASPAS AVVHVLNGE HPAAMQTSR 
      AGVRRQMAL LATFSLLVV TSVR
    • >TC030995 RC
      MITYLTSFT AALVGVSES SKSRFVESV KPIVQRPYF 
      DDVGPRNVT AVVGQSALL NCRVKHPGD RRVSWMRKR 
      DLHILTSDI HTYTGDARF SVRHPEHSD DWDLRIAYV 
      QKRDAGVYE CQVNTEPKI NLAITLNVE DASSLAATH 
      PDPRSKDAQ ATISGPPEV YVKKGSTIS LTCSVNVHS 
      TPPSSVLWY HGASVVDFD SPRGGISLE TEKTEAGTT 
      SKLLVTKAL LSDSGNYTC MPSNASPAS AVVHVLNGE 
      HPAAMQTSR AGVRRQMAL LATFSLLVV TSVR
    CDS sequence
    • >TC030995 RA
      atgaaaata tggttgtgt gcgaaaact ttgcgaaaa 
      gtgtcacaa gattgttta cgaagggtt gacgcctgg 
      gaaactttg cttggaggg ttcataact caacttaca 
      gctgccctg gtgggagtc tcggaaagt tccaagtcc 
      cggtttgtg gaatcggtg aaaccgatc gtgcaaagg 
      ccctatttc gatgacgtc ggccctaga aacgtcacc 
      gccgtcgtg ggccagtca gctttgctc aattgccga 
      gtcaaacat ccaggcgac agaagggtc tcctggatg 
      cgtaaacgc gacctccac atcctgacc tctgacatc 
      cacacatac accggagac gctcgtttc agcgtccgc 
      catccggag cacagcgac gactgggat ctgcgcatt 
      gcttacgtg cagaaacgc gacgccggc gtctacgag 
      tgtcaggtg aacaccgag cccaagatt aatctcgcc 
      atcacgttg aacgtcgag gatgcccag gcgacgatt 
      tccggtccg ccggaagtg tacgtgaag aagggcagc 
      acgatatcg ctcacgtgt tccgtgaac gtccattcg 
      acccctccc tcgtccgtg ctgtggtac catggtgct 
      tccgtggtg gacttcgac tcgcctcgt ggcggcatc 
      agcctcgaa acggaaaaa actgaggca ggcaccacc 
      agcaaatta ctggtcacc aaagctcta ctttccgac 
      tccggaaat tacacttgc atgccgtcc aacgccagt 
      cccgcatcg gccgtcgtt cacgtcctc aatggggag 
      cacccggca gcgatgcag acgagtcgg gcaggcgtg 
      cgacgtcaa atggccctt ttggcgaca ttttctctg 
      ctggtggtg acctcagta agatga
    • >TC030995 RB
      atgaaaata tggttgtgt gcgaaaact ttgcgaaaa 
      gtgtcacaa gattgttta cgaagggtt gacgcctgg 
      gaaactttg cttggaggg ttcataact caacttaca 
      gctgccctg gtgggagtc tcggaaagt tccaagtcc 
      cggtttgtg gaatcggtg aaaccgatc gtgcaaagg 
      ccctatttc gatgacgtc ggccctaga aacgtcacc 
      gccgtcgtg ggccagtca gctttgctc aattgccga 
      gtcaaacat ccaggcgac agaagggtc tcctggatg 
      cgtaaacgc gacctccac atcctgacc tctgacatc 
      cacacatac accggagac gctcgtttc agcgtccgc 
      catccggag cacagcgac gactgggat ctgcgcatt 
      gcttacgtg cagaaacgc gacgccggc gtctacgag 
      tgtcaggtg aacaccgag cccaagatt aatctcgcc 
      atcacgttg aacgtcgag gacgcgtcc tctcttgca 
      gcaacacat ccggaccct cgctccaaa gatgcccag 
      gcgacgatt tccggtccg ccggaagtg tacgtgaag 
      aagggcagc acgatatcg ctcacgtgt tccgtgaac 
      gtccattcg acccctccc tcgtccgtg ctgtggtac 
      catggtgct tccgtggtg gacttcgac tcgcctcgt 
      ggcggcatc agcctcgaa acggaaaaa actgaggca 
      ggcaccacc agcaaatta ctggtcacc aaagctcta 
      ctttccgac tccggaaat tacacttgc atgccgtcc 
      aacgccagt cccgcatcg gccgtcgtt cacgtcctc 
      aatggggag cacccggca gcgatgcag acgagtcgg 
      gcaggcgtg cgacgtcaa atggccctt ttggcgaca 
      ttttctctg ctggtggtg acctcagta agatga
    • >TC030995 RC
      atgattaca tatttgact agttttaca gctgccctg 
      gtgggagtc tcggaaagt tccaagtcc cggtttgtg 
      gaatcggtg aaaccgatc gtgcaaagg ccctatttc 
      gatgacgtc ggccctaga aacgtcacc gccgtcgtg 
      ggccagtca gctttgctc aattgccga gtcaaacat 
      ccaggcgac agaagggtc tcctggatg cgtaaacgc 
      gacctccac atcctgacc tctgacatc cacacatac 
      accggagac gctcgtttc agcgtccgc catccggag 
      cacagcgac gactgggat ctgcgcatt gcttacgtg 
      cagaaacgc gacgccggc gtctacgag tgtcaggtg 
      aacaccgag cccaagatt aatctcgcc atcacgttg 
      aacgtcgag gacgcgtcc tctcttgca gcaacacat 
      ccggaccct cgctccaaa gatgcccag gcgacgatt 
      tccggtccg ccggaagtg tacgtgaag aagggcagc 
      acgatatcg ctcacgtgt tccgtgaac gtccattcg 
      acccctccc tcgtccgtg ctgtggtac catggtgct 
      tccgtggtg gacttcgac tcgcctcgt ggcggcatc 
      agcctcgaa acggaaaaa actgaggca ggcaccacc 
      agcaaatta ctggtcacc aaagctcta ctttccgac 
      tccggaaat tacacttgc atgccgtcc aacgccagt 
      cccgcatcg gccgtcgtt cacgtcctc aatggggag 
      cacccggca gcgatgcag acgagtcgg gcaggcgtg 
      cgacgtcaa atggccctt ttggcgaca ttttctctg 
      ctggtggtg acctcagta agatga
    mRNA sequence
    • >TC030995 RA
      atagataat ttacgacgc aatgaaaat atggttgtg 
      tgcgaaaac tttgcgaaa agtgtcaca agattgttt 
      acgaagggt tgacgcctg ggaaacttt gcttggagg 
      gttcataac tcaacttac agctgccct ggtgggagt 
      ctcggaaag ttccaagtc ccggtttgt ggaatcggt 
      gaaaccgat cgtgcaaag gccctattt cgatgacgt 
      cggccctag aaacgtcac cgccgtcgt gggccagtc 
      agctttgct caattgccg agtcaaaca tccaggcga 
      cagaagggt ctcctggat gcgtaaacg cgacctcca 
      catcctgac ctctgacat ccacacata caccggaga 
      cgctcgttt cagcgtccg ccatccgga gcacagcga 
      cgactggga tctgcgcat tgcttacgt gcagaaacg 
      cgacgccgg cgtctacga gtgtcaggt gaacaccga 
      gcccaagat taatctcgc catcacgtt gaacgtcga 
      ggatgccca ggcgacgat ttccggtcc gccggaagt 
      gtacgtgaa gaagggcag cacgatatc gctcacgtg 
      ttccgtgaa cgtccattc gacccctcc ctcgtccgt 
      gctgtggta ccatggtgc ttccgtggt ggacttcga 
      ctcgcctcg tggcggcat cagcctcga aacggaaaa 
      aactgaggc aggcaccac cagcaaatt actggtcac 
      caaagctct actttccga ctccggaaa ttacacttg 
      catgccgtc caacgccag tcccgcatc ggccgtcgt 
      tcacgtcct caatgggga gcacccggc agcgatgca 
      gacgagtcg ggcaggcgt gcgacgtca aatggccct 
      tttggcgac attttctct gctggtggt gacctcagt 
      aagatgatt acgctgtcc ttaagaaat atatcaatc 
      aacgacacg ctcataacg aactagtgc taaaatcgg 
      tgtaaataa aacaatttt gtgaaaact caggtggtg 
      atatcgacg gatctgatt cgatctttg gtgctaaag 
      tggacataa aaataataa aaggttgtg acgtgacga 
      gggatgaac gtccatttc ttgtatata gtgtgattc 
      atttgatat taatagact caaaagcac tttagatta 
      ttctagcat tattcattg taacaatta ttattacat 
      gttgatatt atgtagatt tattgttat cattaccaa 
      attttatcg gactgggct ttttatttt agtcatcca 
      tggtttgtc tttgctgct ctctcggtg tgaaacttt 
      caatacaac tggaaactt tgaacgatt tattcccgg 
      ctgttttac gtaaaattt taaaacatg tccgacaag 
      aatatattt cgcttagag caactgtgt agtttgatt 
      tatgcaacg acaaacttg gttgaacca caaaatttc 
      ggaaaatca aatcttggc aatattact tcagaaatt 
      gttaactaa tctaacatt ttattcata tttttataa 
      aaatcgttc tacagtgta acaaaaaga atctcaact 
      tgtaattat catcttgta catacaaca ataaaacac 
      aaaatagtt c
    • >TC030995 RB
      atagataat ttacgacgc aatgaaaat atggttgtg 
      tgcgaaaac tttgcgaaa agtgtcaca agattgttt 
      acgaagggt tgacgcctg ggaaacttt gcttggagg 
      gttcataac tcaacttac agctgccct ggtgggagt 
      ctcggaaag ttccaagtc ccggtttgt ggaatcggt 
      gaaaccgat cgtgcaaag gccctattt cgatgacgt 
      cggccctag aaacgtcac cgccgtcgt gggccagtc 
      agctttgct caattgccg agtcaaaca tccaggcga 
      cagaagggt ctcctggat gcgtaaacg cgacctcca 
      catcctgac ctctgacat ccacacata caccggaga 
      cgctcgttt cagcgtccg ccatccgga gcacagcga 
      cgactggga tctgcgcat tgcttacgt gcagaaacg 
      cgacgccgg cgtctacga gtgtcaggt gaacaccga 
      gcccaagat taatctcgc catcacgtt gaacgtcga 
      ggacgcgtc ctctcttgc agcaacaca tccggaccc 
      tcgctccaa agatgccca ggcgacgat ttccggtcc 
      gccggaagt gtacgtgaa gaagggcag cacgatatc 
      gctcacgtg ttccgtgaa cgtccattc gacccctcc 
      ctcgtccgt gctgtggta ccatggtgc ttccgtggt 
      ggacttcga ctcgcctcg tggcggcat cagcctcga 
      aacggaaaa aactgaggc aggcaccac cagcaaatt 
      actggtcac caaagctct actttccga ctccggaaa 
      ttacacttg catgccgtc caacgccag tcccgcatc 
      ggccgtcgt tcacgtcct caatgggga gcacccggc 
      agcgatgca gacgagtcg ggcaggcgt gcgacgtca 
      aatggccct tttggcgac attttctct gctggtggt 
      gacctcagt aagatgatt acgctgtcc ttaagaaat 
      atatcaatc aacgacacg ctcataacg aactagtgc 
      taaaatcgg tgtaaataa aacaatttt gtgaaaact 
      caggtggtg atatcgacg gatctgatt cgatctttg 
      gtgctaaag tggacataa aaataataa aaggttgtg 
      acgtgacga gggatgaac gtccatttc ttgtatata 
      gtgtgattc atttgatat taatagact caaaagcac 
      tttagatta ttctagcat tattcattg taacaatta 
      ttattacat gttgatatt atgtagatt tattgttat 
      cattaccaa attttatcg gactgggct ttttatttt 
      agtcatcca tggtttgtc tttgctgct ctctcggtg 
      tgaaacttt caatacaac tggaaactt tgaacgatt 
      tattcccgg ctgttttac gtaaaattt taaaacatg 
      tccgacaag aatatattt cgcttagag caactgtgt 
      agtttgatt tatgcaacg acaaacttg gttgaacca 
      caaaatttc ggaaaatca aatcttggc aatattact 
      tcagaaatt gttaactaa tctaacatt ttattcata 
      tttttataa aaatcgttc tacagtgta acaaaaaga 
      atctcaact tgtaattat catcttgta catacaaca 
      ataaaacac aaaatagtt c
    • >TC030995 RC
      ctgaaagtc gaaattgaa acacaagcg ccggtccct 
      catgattac atatttgac tagttttac agctgccct 
      ggtgggagt ctcggaaag ttccaagtc ccggtttgt 
      ggaatcggt gaaaccgat cgtgcaaag gccctattt 
      cgatgacgt cggccctag aaacgtcac cgccgtcgt 
      gggccagtc agctttgct caattgccg agtcaaaca 
      tccaggcga cagaagggt ctcctggat gcgtaaacg 
      cgacctcca catcctgac ctctgacat ccacacata 
      caccggaga cgctcgttt cagcgtccg ccatccgga 
      gcacagcga cgactggga tctgcgcat tgcttacgt 
      gcagaaacg cgacgccgg cgtctacga gtgtcaggt 
      gaacaccga gcccaagat taatctcgc catcacgtt 
      gaacgtcga ggacgcgtc ctctcttgc agcaacaca 
      tccggaccc tcgctccaa agatgccca ggcgacgat 
      ttccggtcc gccggaagt gtacgtgaa gaagggcag 
      cacgatatc gctcacgtg ttccgtgaa cgtccattc 
      gacccctcc ctcgtccgt gctgtggta ccatggtgc 
      ttccgtggt ggacttcga ctcgcctcg tggcggcat 
      cagcctcga aacggaaaa aactgaggc aggcaccac 
      cagcaaatt actggtcac caaagctct actttccga 
      ctccggaaa ttacacttg catgccgtc caacgccag 
      tcccgcatc ggccgtcgt tcacgtcct caatgggga 
      gcacccggc agcgatgca gacgagtcg ggcaggcgt 
      gcgacgtca aatggccct tttggcgac attttctct 
      gctggtggt gacctcagt aagatgatt acgctgtcc 
      ttaagaaat atatcaatc aacgacacg ctcataacg 
      aactagtgc taaaatcgg tgtaaataa aacaatttt 
      gtgaaaact caggtggtg atatcgacg gatctgatt 
      cgatctttg gtgctaaag tggacataa aaataataa 
      aaggttgtg acgtgacga gggatgaac gtccatttc 
      ttgtatata gtgtgattc atttgatat taatagact 
      caaaagcac tttagatta ttctagcat tattcattg 
      taacaatta ttattacat gttgatatt atgtagatt 
      tattgttat cattaccaa attttatcg gactgggct 
      ttttatttt agtcatcca tggtttgtc tttgctgct 
      ctctcggtg tgaaacttt caatacaac tggaaactt 
      tgaacgatt tattcccgg ctgttttac gtaaaattt 
      taaaacatg tccgacaag aatatattt cgcttagag 
      caactgtgt agtttgatt tatgcaacg acaaacttg 
      gttgaacca caaaatttc ggaaaatca aatcttggc 
      aatattact tcagaaatt gttaactaa tctaacatt 
      ttattcata tttttataa aaatcgttc tacagtgta 
      acaaaaaga atctcaact tgtaattat catcttgta 
      catacaaca ataaaacac aaaatagtt c