Status
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Gene information
General information
TC000986 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9344578..9346821
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000986
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00202

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 77.78% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000986 RA
      MMPLGGTAP VHSSTNVAP PAPATNNSL TPTGGSNKS 
      SSNLKPNYT LKFTLAGHT KAVSSVKFS PNGEWLASS 
      SADKLIKIW GAYDGKFEK TISGHKLGI SDVAWSSDS 
      RLLVSASDD KTLKIWELS SGKCLKTLK GHSNYVFCC 
      NFNPQSNLI VSGSFDESV RIWDVRTGK CLKTLPAHS 
      DPVSAVHFN RDGSLIVSS SYDGLCRIW DTASGQCLK 
      TLIDDDNPP VSFVKFSPN GKYILAATL DNTLKLWDY 
      AKGKCLKTY SGHKNEKYC IFANFSVTG GKWIVSGSE 
      DNLVYIWNL QTKEIVQKL QGHTDVVLC TTCHPTENI 
      IASAALEHD KTIKLWKSD T
    CDS sequence
    • >TC000986 RA
      atgatgcct ttagggggc acagcccct gtccattct 
      tcgacaaac gttgcccca ccggcccct gctactaac 
      aactccttg acgccgacg ggcggctct aacaagtcc 
      agcagtaac ctaaaacct aactacacg cttaaattc 
      acgctagcg gggcacacc aaggcggtg tcctccgtt 
      aaatttagc ccaaacggc gaatggttg gctagttct 
      tctgcggat aagttaatt aagatatgg ggcgcttac 
      gacggcaag tttgagaag acaatatca gggcataag 
      ttagggata agtgacgtg gcgtggtct agcgatagc 
      agattactt gtatcagca agtgatgat aaaactctt 
      aaaatctgg gaattatca tcagggaag tgcttgaag 
      acgttaaag gggcacagc aattacgtt ttttgttgt 
      aattttaac ccacaatcg aatttaatc gtgtccggt 
      tcgtttgac gaatctgta agaatttgg gatgtgagg 
      actggaaag tgtctaaag acacttcca gcgcactca 
      gaccctgtc agtgcggtt catttcaac agagacggg 
      tctctcatt gtcagcagc agttacgac ggactttgt 
      cgtatttgg gacacagcg tccgggcag tgtcttaag 
      acattaatt gatgatgat aacccccct gtttcgttc 
      gttaaattc tcgccaaac ggcaaatac atcctagcg 
      gcgacttta gacaacacc ctcaaactc tgggattat 
      gccaaaggc aaatgcttg aaaacttac tcagggcat 
      aaaaatgag aaatactgt atttttgca aacttttcc 
      gttactgga ggtaaatgg attgtgagc gggtctgag 
      gataatttg gtctacatt tggaatttg caaacgaag 
      gaaattgta caaaaattg caaggacac acagatgtg 
      gtgctatgt acgacttgc cacccaact gaaaatata 
      atcgcttcg gctgctctc gagcacgac aagaccata 
      aagctatgg aaaagtgat acttaa
    mRNA sequence
    • >TC000986 RA
      aaaaaatta taaaaacat taatagttt tgacttttt 
      gacgttttc taaccccat tttttttct aaccttact 
      tttgacaac gatgtgact tattaacag aaaaaatga 
      tttctgaag actttattc ctacgaggg gttctctca 
      agaaaggta cacaatcca ttgatttta agtcggtgt 
      cctttgaat cgtataagc ccaaaaaac aggaaaaaa 
      ttgaaatta gtaatgaaa agccccaga acaagaaat 
      ccgaagatt tcaacataa ggagagcga aacacgaaa 
      ttgttaaat ttggaatgt caggatttg acccggaaa 
      agaaggaag aagcaaaaa ttcagttag caatcaaat 
      taggagcaa agcccaaga aaaataagt acaaaaact 
      ataaattat taaaggaag aacgggcga aacttaagc 
      aagaagaaa aaacaaagg ctcagttcc agcagttgg 
      gaaaaaacg caattggca aatccacag ctaaaggaa 
      aaggttttg accggaagc gcaaaaagg caaagggtg 
      gattattag acgtgtatg gcaaagtga atttgaaaa 
      acgcgacga aataaatat ttccacatt gaattgttt 
      tttattcaa acctgacgc cattttcta gtgactttt 
      tttgtcaac cggcgttgg cgcattgac gcttgtgat 
      ggcggctga ataaagcag aaattgctt taaaacttg 
      tgacaacaa tcgcaaaaa acgctcttt accgggatt 
      ttcgcggtt taatttcag tgccagtgg cttgttaca 
      attattcag gttagcagc ttcacaata gtggcattt 
      ggaacacct gaatcgcca caaaatgat gcctttagg 
      gggcacagc ccctgtcca ttcttcgac aaacgttgc 
      cccaccggc ccctgctac taacaactc cttgacgcc 
      gacgggcgg ctctaacaa gtccagcag taacctaaa 
      acctaacta cacgcttaa attcacgct agcggggca 
      caccaaggc ggtgtcctc cgttaaatt tagcccaaa 
      cggcgaatg gttggctag ttcttctgc ggataagtt 
      aattaagat atggggcgc ttacgacgg caagtttga 
      gaagacaat atcagggca taagttagg gataagtga 
      cgtggcgtg gtctagcga tagcagatt acttgtatc 
      agcaagtga tgataaaac tcttaaaat ctgggaatt 
      atcatcagg gaagtgctt gaagacgtt aaaggggca 
      cagcaatta cgttttttg ttgtaattt taacccaca 
      atcgaattt aatcgtgtc cggttcgtt tgacgaatc 
      tgtaagaat ttgggatgt gaggactgg aaagtgtct 
      aaagacact tccagcgca ctcagaccc tgtcagtgc 
      ggttcattt caacagaga cgggtctct cattgtcag 
      cagcagtta cgacggact ttgtcgtat ttgggacac 
      agcgtccgg gcagtgtct taagacatt aattgatga 
      tgataaccc ccctgtttc gttcgttaa attctcgcc 
      aaacggcaa atacatcct agcggcgac tttagacaa 
      caccctcaa actctggga ttatgccaa aggcaaatg 
      cttgaaaac ttactcagg gcataaaaa tgagaaata 
      ctgtatttt tgcaaactt ttccgttac tggaggtaa 
      atggattgt gagcgggtc tgaggataa tttggtcta 
      catttggaa tttgcaaac gaaggaaat tgtacaaaa 
      attgcaagg acacacaga tgtggtgct atgtacgac 
      ttgccaccc aactgaaaa tataatcgc ttcggctgc 
      tctcgagca cgacaagac cataaagct atggaaaag 
      tgatactta atccagctt ctacaatgt tttctatga 
      aaaataatt tatattttt ttatgagtg gctaacgat 
      gtaaatttg attcagatt aatgttagg cggtaggag 
      tagccacaa ctaataaac ctttgaatt tttc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY