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Gene information
General information
TC000963 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 8976150..8980403
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000963
    iBeetle screen iB_00198

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature differentiation incomplete - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    labrum size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    labrum not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    gena bristle number increased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000963 RA
      MSGSAHKHR YKNAALDSV ELRRRREEE GLQLRKQKR 
      EQQLNKRRN VNVNQLAED NDHLQVDSE ILSPVSPMI 
      TPEMVQALY SPDVEQQIS ATQKFRQLL SYEPNPPID 
      EVVQTGIIP RFVEFLQNS NNCSLQFEA AWALTNVAS 
      GTSQQTRMV IEAGAVPIF IRLLSSQYE DVQEQAVWA 
      LGNIAGDSP ECRDHVLDS GILVPLLQL LSKSTRLSM 
      TRNAVWALS NLCRGKNPP PDFAKVSPA LPVLARLLF 
      HSDPDVLSD TCWALSYLS DGPNEKIQA VIDAGVCRK 
      LVELLMHQQ PNVVSAALR AVGNIVTGD DVQTQVILN 
      CSALHCLHH LLSSSKESV RKEACWTIS NITAGNRQQ 
      IQAVIDANI FPVLIEILS KAEFKTRKE AAWAITNAT 
      SGGTPDQIR YLVNQACIG PLCDLLTVM DAKIVQVAL 
      NGLENILRL GEQDAKNHS GTNPYAVLI EQCYGLDKI 
      EFLQSHVNM EIYQKAFDI IEHFFGTEE EDTTVAPSV 
      NPDQQQYHF SSDQSVPMG GFHF
    CDS sequence
    • >TC000963 RA
      atgtcgggc tccgctcac aaacaccgc tacaaaaac 
      gcggcgctg gacagcgtc gagttgcga cggcggcga 
      gaggaagaa ggcctccag ttaaggaaa cagaaacgg 
      gaacagcag ctgaacaag cggcggaat gttaacgtc 
      aaccaattg gccgaggac aatgaccat ctgcaagtg 
      gacagcgag atcctctcg cctgtctcg cccatgatc 
      acccccgaa atggtccag gcgttgtac agccccgac 
      gtggagcag caaatcagc gccacgcaa aaattcaga 
      caactgctg agctacgaa cccaaccca cccattgat 
      gaggtggtc cagaccggg atcatccca cgatttgta 
      gagtttttg cagaattca aacaattgt agccttcag 
      tttgaagca gcatgggct ttgaccaac gttgcctct 
      ggtacctct cagcaaacc aggatggtc attgaagct 
      ggtgctgtt cctattttt atacgtttg ttgagcagt 
      caatatgag gatgttcag gagcaagct gtttgggca 
      ttagggaat atagcaggg gattcaccc gaatgtaga 
      gatcacgtc ctggattcg ggtattttg gttccactc 
      ttacaactc cttagtaaa tcgacgcgt ttatcgatg 
      actcgcaat gccgtgtgg gcgctttcg aacctctgc 
      aggggcaag aaccctcct ccagacttt gcgaaagtc 
      tccccagct ctgcccgta ctcgctcgt ctcttgttt 
      cactccgac cctgacgtg ctctctgac acttgctgg 
      gccttgagc tacctatcc gacggtcct aacgaaaaa 
      atccaggcg gtcatagac gccggcgtt tgtcggaaa 
      ctggtcgaa ttgctaatg caccaacag cccaacgta 
      gtctcggca gctctgcgc gctgtcggc aacatagtg 
      actggtgac gacgtccaa actcaggtc atactaaac 
      tgctccgct ctacattgc ttgcaccac ttgttgtca 
      tcctcgaag gagtccgtg aggaaggag gcgtgttgg 
      acgatcagc aacatcacg gctggaaac cggcagcaa 
      atccaggcg gtcatagac gcgaacatt ttcccggtt 
      ttgatagag attttgagc aaggcggag tttaagacg 
      cggaaggag gcggcctgg gctatcacg aatgccacc 
      agcgggggc acacccgac cagatcagg tatctagtg 
      aaccaggct tgcattggg ccgctttgt gaccttttg 
      accgtcatg gacgcgaaa atcgtgcaa gtggccctc 
      aacggcctg gagaatatt ctgagatta ggggagcaa 
      gacgccaag aatcactcg gggaccaac ccgtatgct 
      gtgctaatc gagcagtgc tatgggttg gataaaatc 
      gaatttttg caatcgcat gtcaatatg gagatttat 
      cagaaggcc ttcgacatt attgaacac ttcttcggg 
      acggaggag gaggacacg actgtggcg ccctcggtg 
      aatcccgac caacagcag taccatttc agctcggat 
      cagtcggtg ccgatggga ggattccat ttttaa
    mRNA sequence
    • >TC000963 RA
      ttgcaaata gttccgaat gccagaatt ttcattttc 
      acgattcca tttccaatt atcgctgaa ttcctgatt 
      cgtccaaaa gtatcaaat gtgtgtttc catctgagt 
      gtgagaaaa accgtcgga cattacgga agatgtcgg 
      gctccgctc acaaacacc gctacaaaa acgcggcgc 
      tggacagcg tcgagttgc gacggcggc gagaggaag 
      aaggcctcc agttaagga aacagaaac gggaacagc 
      agctgaaca agcggcgga atgttaacg tcaaccaat 
      tggccgagg acaatgacc atctgcaag tggacagcg 
      agatcctct cgcctgtct cgcccatga tcacccccg 
      aaatggtcc aggcgttgt acagccccg acgtggagc 
      agcaaatca gcgccacgc aaaaattca gacaactgc 
      tgagctacg aacccaacc cacccattg atgaggtgg 
      tccagaccg ggatcatcc cacgatttg tagagtttt 
      tgcagaatt caaacaatt gtagccttc agtttgaag 
      cagcatggg ctttgacca acgttgcct ctggtacct 
      ctcagcaaa ccaggatgg tcattgaag ctggtgctg 
      ttcctattt ttatacgtt tgttgagca gtcaatatg 
      aggatgttc aggagcaag ctgtttggg cattaggga 
      atatagcag gggattcac ccgaatgta gagatcacg 
      tcctggatt cgggtattt tggttccac tcttacaac 
      tccttagta aatcgacgc gtttatcga tgactcgca 
      atgccgtgt gggcgcttt cgaacctct gcaggggca 
      agaaccctc ctccagact ttgcgaaag tctccccag 
      ctctgcccg tactcgctc gtctcttgt ttcactccg 
      accctgacg tgctctctg acacttgct gggccttga 
      gctacctat ccgacggtc ctaacgaaa aaatccagg 
      cggtcatag acgccggcg tttgtcgga aactggtcg 
      aattgctaa tgcaccaac agcccaacg tagtctcgg 
      cagctctgc gcgctgtcg gcaacatag tgactggtg 
      acgacgtcc aaactcagg tcatactaa actgctccg 
      ctctacatt gcttgcacc acttgttgt catcctcga 
      aggagtccg tgaggaagg aggcgtgtt ggacgatca 
      gcaacatca cggctggaa accggcagc aaatccagg 
      cggtcatag acgcgaaca ttttcccgg ttttgatag 
      agattttga gcaaggcgg agtttaaga cgcggaagg 
      aggcggcct gggctatca cgaatgcca ccagcgggg 
      gcacacccg accagatca ggtatctag tgaaccagg 
      cttgcattg ggccgcttt gtgaccttt tgaccgtca 
      tggacgcga aaatcgtgc aagtggccc tcaacggcc 
      tggagaata ttctgagat taggggagc aagacgcca 
      agaatcact cggggacca acccgtatg ctgtgctaa 
      tcgagcagt gctatgggt tggataaaa tcgaatttt 
      tgcaatcgc atgtcaata tggagattt atcagaagg 
      ccttcgaca ttattgaac acttcttcg ggacggagg 
      aggaggaca cgactgtgg cgccctcgg tgaatcccg 
      accaacagc agtaccatt tcagctcgg atcagtcgg 
      tgccgatgg gaggattcc atttttaaa tcgagtttt 
      agattattt taaggactt ggtcacgcc aaaggataa 
      ccttcacga tatccggta tttagtttg aatcaagtg 
      atagtgtta cttcattgt aacagaatt ttatgcatg 
      tatggcgga aatacaaag tcgacagca aaaattatt 
      cttttcgga gataagtgt cttgattct cgaaattcg 
      gttttgtaa aattgcttc gatttttca gaaatgatt 
      gttgtgtaa gtgagctta acatacgct atttatttt 
      atacagagg ccagctttt gtcttcact gatctaggt 
      tttgtaaca gctatgtac atattttac gtaaagtgt 
      gaaacatac atgtataaa tattcctaa cagcatatt 
      tgtttatag tttttattg taaaataaa ctaacattt 
      aagta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY