General information | |||
TC000963 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 8976150..8980403 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC000963 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00198 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
larval musculature differentiation incomplete - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
labrum size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
labrum not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
gena bristle number increased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 3 (41 dpi)
- >TC000963 RA
MSGSAHKHR YKNAALDSV ELRRRREEE GLQLRKQKR EQQLNKRRN VNVNQLAED NDHLQVDSE ILSPVSPMI TPEMVQALY SPDVEQQIS ATQKFRQLL SYEPNPPID EVVQTGIIP RFVEFLQNS NNCSLQFEA AWALTNVAS GTSQQTRMV IEAGAVPIF IRLLSSQYE DVQEQAVWA LGNIAGDSP ECRDHVLDS GILVPLLQL LSKSTRLSM TRNAVWALS NLCRGKNPP PDFAKVSPA LPVLARLLF HSDPDVLSD TCWALSYLS DGPNEKIQA VIDAGVCRK LVELLMHQQ PNVVSAALR AVGNIVTGD DVQTQVILN CSALHCLHH LLSSSKESV RKEACWTIS NITAGNRQQ IQAVIDANI FPVLIEILS KAEFKTRKE AAWAITNAT SGGTPDQIR YLVNQACIG PLCDLLTVM DAKIVQVAL NGLENILRL GEQDAKNHS GTNPYAVLI EQCYGLDKI EFLQSHVNM EIYQKAFDI IEHFFGTEE EDTTVAPSV NPDQQQYHF SSDQSVPMG GFHF
- >TC000963 RA
atgtcgggc tccgctcac aaacaccgc tacaaaaac gcggcgctg gacagcgtc gagttgcga cggcggcga gaggaagaa ggcctccag ttaaggaaa cagaaacgg gaacagcag ctgaacaag cggcggaat gttaacgtc aaccaattg gccgaggac aatgaccat ctgcaagtg gacagcgag atcctctcg cctgtctcg cccatgatc acccccgaa atggtccag gcgttgtac agccccgac gtggagcag caaatcagc gccacgcaa aaattcaga caactgctg agctacgaa cccaaccca cccattgat gaggtggtc cagaccggg atcatccca cgatttgta gagtttttg cagaattca aacaattgt agccttcag tttgaagca gcatgggct ttgaccaac gttgcctct ggtacctct cagcaaacc aggatggtc attgaagct ggtgctgtt cctattttt atacgtttg ttgagcagt caatatgag gatgttcag gagcaagct gtttgggca ttagggaat atagcaggg gattcaccc gaatgtaga gatcacgtc ctggattcg ggtattttg gttccactc ttacaactc cttagtaaa tcgacgcgt ttatcgatg actcgcaat gccgtgtgg gcgctttcg aacctctgc aggggcaag aaccctcct ccagacttt gcgaaagtc tccccagct ctgcccgta ctcgctcgt ctcttgttt cactccgac cctgacgtg ctctctgac acttgctgg gccttgagc tacctatcc gacggtcct aacgaaaaa atccaggcg gtcatagac gccggcgtt tgtcggaaa ctggtcgaa ttgctaatg caccaacag cccaacgta gtctcggca gctctgcgc gctgtcggc aacatagtg actggtgac gacgtccaa actcaggtc atactaaac tgctccgct ctacattgc ttgcaccac ttgttgtca tcctcgaag gagtccgtg aggaaggag gcgtgttgg acgatcagc aacatcacg gctggaaac cggcagcaa atccaggcg gtcatagac gcgaacatt ttcccggtt ttgatagag attttgagc aaggcggag tttaagacg cggaaggag gcggcctgg gctatcacg aatgccacc agcgggggc acacccgac cagatcagg tatctagtg aaccaggct tgcattggg ccgctttgt gaccttttg accgtcatg gacgcgaaa atcgtgcaa gtggccctc aacggcctg gagaatatt ctgagatta ggggagcaa gacgccaag aatcactcg gggaccaac ccgtatgct gtgctaatc gagcagtgc tatgggttg gataaaatc gaatttttg caatcgcat gtcaatatg gagatttat cagaaggcc ttcgacatt attgaacac ttcttcggg acggaggag gaggacacg actgtggcg ccctcggtg aatcccgac caacagcag taccatttc agctcggat cagtcggtg ccgatggga ggattccat ttttaa
- >TC000963 RA
ttgcaaata gttccgaat gccagaatt ttcattttc acgattcca tttccaatt atcgctgaa ttcctgatt cgtccaaaa gtatcaaat gtgtgtttc catctgagt gtgagaaaa accgtcgga cattacgga agatgtcgg gctccgctc acaaacacc gctacaaaa acgcggcgc tggacagcg tcgagttgc gacggcggc gagaggaag aaggcctcc agttaagga aacagaaac gggaacagc agctgaaca agcggcgga atgttaacg tcaaccaat tggccgagg acaatgacc atctgcaag tggacagcg agatcctct cgcctgtct cgcccatga tcacccccg aaatggtcc aggcgttgt acagccccg acgtggagc agcaaatca gcgccacgc aaaaattca gacaactgc tgagctacg aacccaacc cacccattg atgaggtgg tccagaccg ggatcatcc cacgatttg tagagtttt tgcagaatt caaacaatt gtagccttc agtttgaag cagcatggg ctttgacca acgttgcct ctggtacct ctcagcaaa ccaggatgg tcattgaag ctggtgctg ttcctattt ttatacgtt tgttgagca gtcaatatg aggatgttc aggagcaag ctgtttggg cattaggga atatagcag gggattcac ccgaatgta gagatcacg tcctggatt cgggtattt tggttccac tcttacaac tccttagta aatcgacgc gtttatcga tgactcgca atgccgtgt gggcgcttt cgaacctct gcaggggca agaaccctc ctccagact ttgcgaaag tctccccag ctctgcccg tactcgctc gtctcttgt ttcactccg accctgacg tgctctctg acacttgct gggccttga gctacctat ccgacggtc ctaacgaaa aaatccagg cggtcatag acgccggcg tttgtcgga aactggtcg aattgctaa tgcaccaac agcccaacg tagtctcgg cagctctgc gcgctgtcg gcaacatag tgactggtg acgacgtcc aaactcagg tcatactaa actgctccg ctctacatt gcttgcacc acttgttgt catcctcga aggagtccg tgaggaagg aggcgtgtt ggacgatca gcaacatca cggctggaa accggcagc aaatccagg cggtcatag acgcgaaca ttttcccgg ttttgatag agattttga gcaaggcgg agtttaaga cgcggaagg aggcggcct gggctatca cgaatgcca ccagcgggg gcacacccg accagatca ggtatctag tgaaccagg cttgcattg ggccgcttt gtgaccttt tgaccgtca tggacgcga aaatcgtgc aagtggccc tcaacggcc tggagaata ttctgagat taggggagc aagacgcca agaatcact cggggacca acccgtatg ctgtgctaa tcgagcagt gctatgggt tggataaaa tcgaatttt tgcaatcgc atgtcaata tggagattt atcagaagg ccttcgaca ttattgaac acttcttcg ggacggagg aggaggaca cgactgtgg cgccctcgg tgaatcccg accaacagc agtaccatt tcagctcgg atcagtcgg tgccgatgg gaggattcc atttttaaa tcgagtttt agattattt taaggactt ggtcacgcc aaaggataa ccttcacga tatccggta tttagtttg aatcaagtg atagtgtta cttcattgt aacagaatt ttatgcatg tatggcgga aatacaaag tcgacagca aaaattatt cttttcgga gataagtgt cttgattct cgaaattcg gttttgtaa aattgcttc gatttttca gaaatgatt gttgtgtaa gtgagctta acatacgct atttatttt atacagagg ccagctttt gtcttcact gatctaggt tttgtaaca gctatgtac atattttac gtaaagtgt gaaacatac atgtataaa tattcctaa cagcatatt tgtttatag tttttattg taaaataaa ctaacattt aagta
