Status
Please wait ...
Gene information
General information
TC000949 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 3304162..3305852
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000949
    iBeetle screen iB_00195
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000949 RA
      MERKSDLGE PSLKNRRKC SPQMPENSC QIMVDNSQT 
      VLNILRNLY VERQMCDVA LRVGNREHY AHRLILCAF 
      SDVFQAMLM KPEWCEWHE SKVELQELL PECEKVFHL 
      FLEYFYTGK ILITHTNVM PILALADKY IVKGLSRLC 
      VDYMCKHVP HAASHNQLF LWLQYSIPC GHNEVGEKC 
      QNYIKWNLE SIANTPDFS NIDAEMLIQ LLQENDVIV 
      YNEMVLYNC VVRWLDLQK IKLHSSEMS ANEVENYMR 
      ELVKNIMIY IRFPMMTPR ELADLLLSP LIKQYKEFF 
      VDRMAIGMI YHSGQLEQI EKISQTQEG RLLFSPRLY 
      TSDTYSAVL LIENFNSLP SYHTSTFVF SSHMSAADC 
      ESDKINDWV VDLYPKGVW FKKCYLIVW QGTLEVPEE 
      IISTVRLSL TCRELLQAK IKVKVAVLI YGIQGGVEH 
      VMEVREKIH HFTNDDKVM NIDNLIPFC ELNPCASTN 
      SHDSPYLIG PNRNQLKLN IVITSLT
    CDS sequence
    • >TC000949 RA
      atggagcgc aagagtgac ttgggggag ccctcgttg 
      aaaaacagg cgcaaatgc tccccccaa atgcccgaa 
      aattcgtgc cagattatg gttgacaac tcgcagact 
      gttttaaac attttacgc aacttgtac gtggagagg 
      cagatgtgc gacgtggct ctgcgggtc ggcaaccgc 
      gagcactat gcccaccgt ctcatcctg tgcgctttc 
      agcgacgtt ttccaggca atgcttatg aagcctgag 
      tggtgcgaa tggcatgag agtaaggtc gagctccag 
      gaattgctc cccgagtgc gaaaaagtg ttccattta 
      ttcttagag tatttttat accgggaaa attctaatt 
      acacatact aacgtaatg ccaatctta gcgctagct 
      gataagtat attgtgaag ggattgtcg cgactttgc 
      gtggattac atgtgcaaa cacgtccca catgccgcc 
      tcacacaac cagctgttc ctctggctg cagtattcc 
      attccgtgc ggtcacaac gaagtcggg gaaaaatgc 
      caaaactac atcaaatgg aacctagaa tccatcgca 
      aatactccc gatttcagc aacattgac gcggaaatg 
      ctcatacag ctgttgcaa gagaacgac gtcattgtc 
      tacaatgaa atggttctt tacaactgc gtggtgcga 
      tggttggac ttgcaaaaa attaagtta cacagctcc 
      gaaatgagc gctaacgaa gtggagaat tacatgcga 
      gagctggtg aaaaacata atgatctat ataagattc 
      ccgatgatg acgccgcga gagctcgcc gatttgttg 
      ctatcgcct ttgataaaa caatacaag gagtttttc 
      gtcgataga atggccatt ggcatgatt taccactcg 
      gggcagttg gagcaaatc gaaaaaatc agccaaacg 
      caagagggg aggctgctc ttcagcccc cggctgtac 
      acttcagac acgtacagt gcggttcta ttaatcgag 
      aacttcaac agtttgccc agttatcac acgagcact 
      tttgttttt tcgtcgcat atgtcggcg gcagattgc 
      gagtcggat aaaatcaat gattgggtc gtggatttg 
      tatcccaaa ggagtttgg tttaaaaaa tgctacttg 
      attgtatgg cagggcacg ctggaggtg cctgaagag 
      atcatttca acagtgaga ttatcgcta acttgtcgc 
      gaactttta caagcgaaa attaaagtc aaagttgcc 
      gtattaatt tacggcatt caaggaggc gtggagcat 
      gttatggaa gtcagagaa aaaatccac catttcact 
      aatgacgac aaagttatg aatattgat aatttaatt 
      ccgttttgt gaactcaat ccgtgcgct tcaaccaat 
      agtcatgac agtccctac ttgattgga cctaataga 
      aatcagctc aaacttaat atagttatt acttcatta 
      acttga
    mRNA sequence
    • >TC000949 RA
      actcactaa ctagtccaa atggagcgc aagagtgac 
      ttgggggag ccctcgttg aaaaacagg cgcaaatgc 
      tccccccaa atgcccgaa aattcgtgc cagattatg 
      gttgacaac tcgcagact gttttaaac attttacgc 
      aacttgtac gtggagagg cagatgtgc gacgtggct 
      ctgcgggtc ggcaaccgc gagcactat gcccaccgt 
      ctcatcctg tgcgctttc agcgacgtt ttccaggca 
      atgcttatg aagcctgag tggtgcgaa tggcatgag 
      agtaaggtc gagctccag gaattgctc cccgagtgc 
      gaaaaagtg ttccattta ttcttagag tatttttat 
      accgggaaa attctaatt acacatact aacgtaatg 
      ccaatctta gcgctagct gataagtat attgtgaag 
      ggattgtcg cgactttgc gtggattac atgtgcaaa 
      cacgtccca catgccgcc tcacacaac cagctgttc 
      ctctggctg cagtattcc attccgtgc ggtcacaac 
      gaagtcggg gaaaaatgc caaaactac atcaaatgg 
      aacctagaa tccatcgca aatactccc gatttcagc 
      aacattgac gcggaaatg ctcatacag ctgttgcaa 
      gagaacgac gtcattgtc tacaatgaa atggttctt 
      tacaactgc gtggtgcga tggttggac ttgcaaaaa 
      attaagtta cacagctcc gaaatgagc gctaacgaa 
      gtggagaat tacatgcga gagctggtg aaaaacata 
      atgatctat ataagattc ccgatgatg acgccgcga 
      gagctcgcc gatttgttg ctatcgcct ttgataaaa 
      caatacaag gagtttttc gtcgataga atggccatt 
      ggcatgatt taccactcg gggcagttg gagcaaatc 
      gaaaaaatc agccaaacg caagagggg aggctgctc 
      ttcagcccc cggctgtac acttcagac acgtacagt 
      gcggttcta ttaatcgag aacttcaac agtttgccc 
      agttatcac acgagcact tttgttttt tcgtcgcat 
      atgtcggcg gcagattgc gagtcggat aaaatcaat 
      gattgggtc gtggatttg tatcccaaa ggagtttgg 
      tttaaaaaa tgctacttg attgtatgg cagggcacg 
      ctggaggtg cctgaagag atcatttca acagtgaga 
      ttatcgcta acttgtcgc gaactttta caagcgaaa 
      attaaagtc aaagttgcc gtattaatt tacggcatt 
      caaggaggc gtggagcat gttatggaa gtcagagaa 
      aaaatccac catttcact aatgacgac aaagttatg 
      aatattgat aatttaatt ccgttttgt gaactcaat 
      ccgtgcgct tcaaccaat agtcatgac agtccctac 
      ttgattgga cctaataga aatcagctc aaacttaat 
      atagttatt acttcatta acttgaacg ccaaatttt 
      gtttggtaa cgttgtaag tacaatttt ttatttttt 
      gcagaatct aatatattt tgttctata aa