Status
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Gene information
General information
TC032116
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000946,TC000947 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 8702500..8727186
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032116
    iBeetle screen iB_00194

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg condensed embryonic tissue, no cuticle, cns fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule, hours 100
    Adult morphology
    remarks: later than schedule, hours 100
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032116 RA
      MRRAKTLYD HGSARLALL AGPSPNRWR ESLLSLPKK 
WLSGTSLVP PSSTTASTE ELPLPKLPV PELEQTMNK 
YEYTMRPLL NTEEQRKLK NLIEKFAGP GGLGTQLQL 
YLQDRREKM DNWAYDYWL DDMYLKNPS SLPVNSNPG 
MVFPPRKFT TILDVARFT ARLLDAALD HKTYLDKKQ 
LPVELATSR EPGQPLCMA QYYRILGTC RIPGRKKDA 
QVVPGKSES EHVIVICRS QLYCVPVQA PDRGRLSED 
EICAQLLHI LDDAPCLAS PPPVGLLTA WKRPLWAEA 
RENLMGSER NRRNLDLIQ RSLLVVCLD EGLGSGFNC 
RLQRGGKGH SAGHRDETN LAVQMIHGG GSTCDTANR 
WFDKTIQLI VSGDGACGL CYEHSQAEG IAVIQLVEK 
LWKHADSQP ATSEVPSAS ISHLPPPER LEWTLDDKD 
FSRIEEAAL SIDNLVKDL DFQVFRYTN YGKDFIKSC 
KVSPDVYIQ LALQLAYYR LYGKLTATY ESASTRRFR 
LGRVDCIRS ASPEALAWA MAMAQPKDE DDSKKVTFH 
LVSDEEKLR LWKEAIKRQ TTEMVDNIL GQGIDIHLL 
GLREAARET SPTAAHPLP DIFTDYTYK LANKFLLST 
SQVATSTDS FMGYGPVEP DGYGASYNP KPDHIIFCL 
SAFWSSESC LSEKFPVMF KNTFQSGFL SILYSIGSK 
PLQIWDKKV KNGHIKRIT DEDIQSLVL EVVGCNVST 
TYITCPADP RKTLGIKLP YLVMIVKNL KKYFTFEVQ 
ILDDKNVRR RFRASNYQS TTRVKPFIC TMPMRLDEG 
WNQIQFNVA DFTRRAYGT NYVETLRVQ VHANCRIRR 
VYFSDRLYS EDELPAEFK LFLPIQNKT RTAVAAQ
    CDS sequence
    • >TC032116 RA
      atgaggagg gctaaaacc ctctacgac cacggatca 
gcgagattg gctttgtta gccggcccc agccccaac 
cgatggcgc gaatcgctg ctttcgcta ccgaagaag 
tggctgtcc gggacgtct ctggtgccc cccagctcg 
accacggct agcactgag gagctcccc ctgcccaag 
ctccccgtg ccggagtta gaacaaacc atgaacaag 
tatgagtac acaatgaga ccgctcctg aacacggaa 
gaacaaaga aagctgaaa aacctcatc gagaaattc 
gctggacca ggcggactt gggacgcag ctacagctc 
tatctgcag gatcggaga gagaaaatg gataattgg 
gcttacgac tactggctc gacgacatg tacctaaaa 
aacccatcc tccctccct gtaaactca aacccgggc 
atggtattc ccgccccgg aaattcacc acaatcctg 
gacgtggcc cgcttcacg gcccgcctt ctggacgca 
gcactggac cacaaaacc tatctagac aagaagcaa 
ctcccggtg gaactagca acgtcccgt gaacccggc 
cagccgctt tgcatggcc cagtactac cgcattttg 
ggcacttgc cggattccg ggccggaaa aaggacgca 
caggtggtt ccgggcaag agcgagtcg gaacacgtg 
atcgtaatc tgccggagc cagctgtac tgcgttccg 
gtgcaggcc ccggaccgg ggccgcttg agcgaggac 
gagatatgc gcccagttg ttgcacatt ttggacgac 
gcgccttgt ctggccagc ccgccgccc gtgggcctc 
ctgacggcc tggaagcgc ccgctgtgg gccgaagcg 
cgcgaaaat ttgatgggg agcgagcgg aatcggcgc 
aatttggat ttgatccag cggtcgttg ctggtggtt 
tgtctggat gaggggctg gggagtggg tttaactgt 
cggttgcaa cgcgggggc aaagggcac agtgccggg 
cacagggat gagaccaat ttagccgtc cagatgata 
cacggaggt ggcagtacg tgcgatact gccaatcga 
tggtttgat aaaaccatc cagttgatt gtgtctgga 
gatggtgct tgtggcttg tgctacgag cattcgcaa 
gccgaagga atcgccgtg atacagctt gtggagaag 
ttgtggaag catgcagac tctcaacca gctacctcc 
gaagtacct tcggcttcg ataagccat ttgccgccg 
cccgagcgc ctggaatgg acgctggac gacaaggac 
ttttcccgc atcgaggaa gcggctctt tccatcgac 
aatttagtc aaagactta gatttccaa gtgttcaga 
tatacgaat tacggcaag gactttatc aagtcctgc 
aaagtcagt ccagacgtc tacatccaa ctcgcacta 
caactagct tactataga ctctacggc aaattgacc 
gcgacctac gaaagcgcc tcaactcgc agattccgc 
ctaggtcga gtggactgc atccggtcc gcgagcccc 
gaggccctg gcctgggcc atggccatg gcccagccc 
aaggacgaa gacgacagc aagaaagta accttccac 
ttggtcagc gacgaggag aagctccgg ctgtggaag 
gaggccatc aagcgccaa accaccgag atggtggac 
aatatcctg ggccaaggc atcgacatc cacctgctg 
ggcttgcgg gaggctgcc agggagacc tcaccgact 
gctgcgcac ccactgcca gacatcttc accgattac 
acttacaag ttagctaac aaatttctc ctaagtacg 
agtcaagtc gcgacctcg acggacagc ttcatgggg 
tacggccca gtggagccg gacgggtac ggagcctcg 
tataacccg aaacccgac catattatt ttctgcttg 
tcggcgttt tggtccagc gaatcatgt ttgtccgaa 
aaattcccc gtaatgttt aaaaacacc ttccaaagt 
ggctttctc tcgattctg tacagtatc ggtagtaag 
ccgcttcag atctgggat aagaaagta aaaaacggc 
cacattaaa aggataacc gatgaggat atccagtcc 
ctggtcctg gaagtggtc gggtgtaat gttagcacc 
acttacatc acctgcccc gccgacccc aggaagacc 
ctaggaatc aaactccct tacttagtc atgattgtg 
aaaaatctc aaaaaatat ttcactttt gaagtccaa 
attttagac gataagaac gtacgacgg aggttcagg 
gctagcaat tatcagtca acaacaagg gtcaagccg 
tttatttgt acaatgcca atgaggcta gacgaaggc 
tggaaccaa atccagttc aatgtggct gattttaca 
cgacgtgcc tatgggacc aactatgtg gagacgcta 
agggtccaa gtccatgcc aactgccgc ataagacga 
gtctacttc tcggacaga ttgtattca gaagacgaa 
cttccagcc gaatttaaa ctgttccta ccaatccaa 
aataagaca cgaactgcg gttgcagcg cagtaa
    mRNA sequence
    • >TC032116 RA
      ggttgaatg aaaggagaa gtgcggcgc tttgtcgaa 
cgagcgggt gccgagtca attgggcat gaggagggc 
taaaaccct ctacgacca cggatcagc gagattggc 
tttgttagc cggccccag ccccaaccg atggcgcga 
atcgctgct ttcgctacc gaagaagtg gctgtccgg 
gacgtctct ggtgccccc cagctcgac cacggctag 
cactgagga gctccccct gcccaagct ccccgtgcc 
ggagttaga acaaaccat gaacaagta tgagtacac 
aatgagacc gctcctgaa cacggaaga acaaagaaa 
gctgaaaaa cctcatcga gaaattcgc tggaccagg 
cggacttgg gacgcagct acagctcta tctgcagga 
tcggagaga gaaaatgga taattgggc ttacgacta 
ctggctcga cgacatgta cctaaaaaa cccatcctc 
cctccctgt aaactcaaa cccgggcat ggtattccc 
gccccggaa attcaccac aatcctgga cgtggcccg 
cttcacggc ccgccttct ggacgcagc actggacca 
caaaaccta tctagacaa gaagcaact cccggtgga 
actagcaac gtcccgtga acccggcca gccgctttg 
catggccca gtactaccg cattttggg cacttgccg 
gattccggg ccggaaaaa ggacgcaca ggtggttcc 
gggcaagag cgagtcgga acacgtgat cgtaatctg 
ccggagcca gctgtactg cgttccggt gcaggcccc 
ggaccgggg ccgcttgag cgaggacga gatatgcgc 
ccagttgtt gcacatttt ggacgacgc gccttgtct 
ggccagccc gccgcccgt gggcctcct gacggcctg 
gaagcgccc gctgtgggc cgaagcgcg cgaaaattt 
gatggggag cgagcggaa tcggcgcaa tttggattt 
gatccagcg gtcgttgct ggtggtttg tctggatga 
ggggctggg gagtgggtt taactgtcg gttgcaacg 
cgggggcaa agggcacag tgccgggca cagggatga 
gaccaattt agccgtcca gatgataca cggaggtgg 
cagtacgtg cgatactgc caatcgatg gtttgataa 
aaccatcca gttgattgt gtctggaga tggtgcttg 
tggcttgtg ctacgagca ttcgcaagc cgaaggaat 
cgccgtgat acagcttgt ggagaagtt gtggaagca 
tgcagactc tcaaccagc tacctccga agtaccttc 
ggcttcgat aagccattt gccgccgcc cgagcgcct 
ggaatggac gctggacga caaggactt ttcccgcat 
cgaggaagc ggctctttc catcgacaa tttagtcaa 
agacttaga tttccaagt gttcagata tacgaatta 
cggcaagga ctttatcaa gtcctgcaa agtcagtcc 
agacgtcta catccaact cgcactaca actagctta 
ctatagact ctacggcaa attgaccgc gacctacga 
aagcgcctc aactcgcag attccgcct aggtcgagt 
ggactgcat ccggtccgc gagccccga ggccctggc 
ctgggccat ggccatggc ccagcccaa ggacgaaga 
cgacagcaa gaaagtaac cttccactt ggtcagcga 
cgaggagaa gctccggct gtggaagga ggccatcaa 
gcgccaaac caccgagat ggtggacaa tatcctggg 
ccaaggcat cgacatcca cctgctggg cttgcggga 
ggctgccag ggagacctc accgactgc tgcgcaccc 
actgccaga catcttcac cgattacac ttacaagtt 
agctaacaa atttctcct aagtacgag tcaagtcgc 
gacctcgac ggacagctt catggggta cggcccagt 
ggagccgga cgggtacgg agcctcgta taacccgaa 
acccgacca tattatttt ctgcttgtc ggcgttttg 
gtccagcga atcatgttt gtccgaaaa attccccgt 
aatgtttaa aaacacctt ccaaagtgg ctttctctc 
gattctgta cagtatcgg tagtaagcc gcttcagat 
ctgggataa gaaagtaaa aaacggcca cattaaaag 
gataaccga tgaggatat ccagtccct ggtcctgga 
agtggtcgg gtgtaatgt tagcaccac ttacatcac 
ctgccccgc cgaccccag gaagaccct aggaatcaa 
actccctta cttagtcat gattgtgaa aaatctcaa 
aaaatattt cacttttga agtccaaat tttagacga 
taagaacgt acgacggag gttcagggc tagcaatta 
tcagtcaac aacaagggt caagccgtt tatttgtac 
aatgccaat gaggctaga cgaaggctg gaaccaaat 
ccagttcaa tgtggctga ttttacacg acgtgccta 
tgggaccaa ctatgtgga gacgctaag ggtccaagt 
ccatgccaa ctgccgcat aagacgagt ctacttctc 
ggacagatt gtattcaga agacgaact tccagccga 
atttaaact gttcctacc aatccaaaa taagacacg 
aactgcggt tgcagcgca gtaaacatt ttaggaccg 
gacattagg tttttttgt tgtaatttg aacaaattg 
ctttttaaa ctcattagg taacacaaa attgtatgc 
aatcatcat tttagacac aataaactt catctttca 
ca
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