| General information | |||
| TC032116
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 8702500..8727186 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC032116 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00194 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC032116 RA
MRRAKTLYD HGSARLALL AGPSPNRWR ESLLSLPKK WLSGTSLVP PSSTTASTE ELPLPKLPV PELEQTMNK YEYTMRPLL NTEEQRKLK NLIEKFAGP GGLGTQLQL YLQDRREKM DNWAYDYWL DDMYLKNPS SLPVNSNPG MVFPPRKFT TILDVARFT ARLLDAALD HKTYLDKKQ LPVELATSR EPGQPLCMA QYYRILGTC RIPGRKKDA QVVPGKSES EHVIVICRS QLYCVPVQA PDRGRLSED EICAQLLHI LDDAPCLAS PPPVGLLTA WKRPLWAEA RENLMGSER NRRNLDLIQ RSLLVVCLD EGLGSGFNC RLQRGGKGH SAGHRDETN LAVQMIHGG GSTCDTANR WFDKTIQLI VSGDGACGL CYEHSQAEG IAVIQLVEK LWKHADSQP ATSEVPSAS ISHLPPPER LEWTLDDKD FSRIEEAAL SIDNLVKDL DFQVFRYTN YGKDFIKSC KVSPDVYIQ LALQLAYYR LYGKLTATY ESASTRRFR LGRVDCIRS ASPEALAWA MAMAQPKDE DDSKKVTFH LVSDEEKLR LWKEAIKRQ TTEMVDNIL GQGIDIHLL GLREAARET SPTAAHPLP DIFTDYTYK LANKFLLST SQVATSTDS FMGYGPVEP DGYGASYNP KPDHIIFCL SAFWSSESC LSEKFPVMF KNTFQSGFL SILYSIGSK PLQIWDKKV KNGHIKRIT DEDIQSLVL EVVGCNVST TYITCPADP RKTLGIKLP YLVMIVKNL KKYFTFEVQ ILDDKNVRR RFRASNYQS TTRVKPFIC TMPMRLDEG WNQIQFNVA DFTRRAYGT NYVETLRVQ VHANCRIRR VYFSDRLYS EDELPAEFK LFLPIQNKT RTAVAAQ
- >TC032116 RA
atgaggagg gctaaaacc ctctacgac cacggatca gcgagattg gctttgtta gccggcccc agccccaac cgatggcgc gaatcgctg ctttcgcta ccgaagaag tggctgtcc gggacgtct ctggtgccc cccagctcg accacggct agcactgag gagctcccc ctgcccaag ctccccgtg ccggagtta gaacaaacc atgaacaag tatgagtac acaatgaga ccgctcctg aacacggaa gaacaaaga aagctgaaa aacctcatc gagaaattc gctggacca ggcggactt gggacgcag ctacagctc tatctgcag gatcggaga gagaaaatg gataattgg gcttacgac tactggctc gacgacatg tacctaaaa aacccatcc tccctccct gtaaactca aacccgggc atggtattc ccgccccgg aaattcacc acaatcctg gacgtggcc cgcttcacg gcccgcctt ctggacgca gcactggac cacaaaacc tatctagac aagaagcaa ctcccggtg gaactagca acgtcccgt gaacccggc cagccgctt tgcatggcc cagtactac cgcattttg ggcacttgc cggattccg ggccggaaa aaggacgca caggtggtt ccgggcaag agcgagtcg gaacacgtg atcgtaatc tgccggagc cagctgtac tgcgttccg gtgcaggcc ccggaccgg ggccgcttg agcgaggac gagatatgc gcccagttg ttgcacatt ttggacgac gcgccttgt ctggccagc ccgccgccc gtgggcctc ctgacggcc tggaagcgc ccgctgtgg gccgaagcg cgcgaaaat ttgatgggg agcgagcgg aatcggcgc aatttggat ttgatccag cggtcgttg ctggtggtt tgtctggat gaggggctg gggagtggg tttaactgt cggttgcaa cgcgggggc aaagggcac agtgccggg cacagggat gagaccaat ttagccgtc cagatgata cacggaggt ggcagtacg tgcgatact gccaatcga tggtttgat aaaaccatc cagttgatt gtgtctgga gatggtgct tgtggcttg tgctacgag cattcgcaa gccgaagga atcgccgtg atacagctt gtggagaag ttgtggaag catgcagac tctcaacca gctacctcc gaagtacct tcggcttcg ataagccat ttgccgccg cccgagcgc ctggaatgg acgctggac gacaaggac ttttcccgc atcgaggaa gcggctctt tccatcgac aatttagtc aaagactta gatttccaa gtgttcaga tatacgaat tacggcaag gactttatc aagtcctgc aaagtcagt ccagacgtc tacatccaa ctcgcacta caactagct tactataga ctctacggc aaattgacc gcgacctac gaaagcgcc tcaactcgc agattccgc ctaggtcga gtggactgc atccggtcc gcgagcccc gaggccctg gcctgggcc atggccatg gcccagccc aaggacgaa gacgacagc aagaaagta accttccac ttggtcagc gacgaggag aagctccgg ctgtggaag gaggccatc aagcgccaa accaccgag atggtggac aatatcctg ggccaaggc atcgacatc cacctgctg ggcttgcgg gaggctgcc agggagacc tcaccgact gctgcgcac ccactgcca gacatcttc accgattac acttacaag ttagctaac aaatttctc ctaagtacg agtcaagtc gcgacctcg acggacagc ttcatgggg tacggccca gtggagccg gacgggtac ggagcctcg tataacccg aaacccgac catattatt ttctgcttg tcggcgttt tggtccagc gaatcatgt ttgtccgaa aaattcccc gtaatgttt aaaaacacc ttccaaagt ggctttctc tcgattctg tacagtatc ggtagtaag ccgcttcag atctgggat aagaaagta aaaaacggc cacattaaa aggataacc gatgaggat atccagtcc ctggtcctg gaagtggtc gggtgtaat gttagcacc acttacatc acctgcccc gccgacccc aggaagacc ctaggaatc aaactccct tacttagtc atgattgtg aaaaatctc aaaaaatat ttcactttt gaagtccaa attttagac gataagaac gtacgacgg aggttcagg gctagcaat tatcagtca acaacaagg gtcaagccg tttatttgt acaatgcca atgaggcta gacgaaggc tggaaccaa atccagttc aatgtggct gattttaca cgacgtgcc tatgggacc aactatgtg gagacgcta agggtccaa gtccatgcc aactgccgc ataagacga gtctacttc tcggacaga ttgtattca gaagacgaa cttccagcc gaatttaaa ctgttccta ccaatccaa aataagaca cgaactgcg gttgcagcg cagtaa
- >TC032116 RA
ggttgaatg aaaggagaa gtgcggcgc tttgtcgaa cgagcgggt gccgagtca attgggcat gaggagggc taaaaccct ctacgacca cggatcagc gagattggc tttgttagc cggccccag ccccaaccg atggcgcga atcgctgct ttcgctacc gaagaagtg gctgtccgg gacgtctct ggtgccccc cagctcgac cacggctag cactgagga gctccccct gcccaagct ccccgtgcc ggagttaga acaaaccat gaacaagta tgagtacac aatgagacc gctcctgaa cacggaaga acaaagaaa gctgaaaaa cctcatcga gaaattcgc tggaccagg cggacttgg gacgcagct acagctcta tctgcagga tcggagaga gaaaatgga taattgggc ttacgacta ctggctcga cgacatgta cctaaaaaa cccatcctc cctccctgt aaactcaaa cccgggcat ggtattccc gccccggaa attcaccac aatcctgga cgtggcccg cttcacggc ccgccttct ggacgcagc actggacca caaaaccta tctagacaa gaagcaact cccggtgga actagcaac gtcccgtga acccggcca gccgctttg catggccca gtactaccg cattttggg cacttgccg gattccggg ccggaaaaa ggacgcaca ggtggttcc gggcaagag cgagtcgga acacgtgat cgtaatctg ccggagcca gctgtactg cgttccggt gcaggcccc ggaccgggg ccgcttgag cgaggacga gatatgcgc ccagttgtt gcacatttt ggacgacgc gccttgtct ggccagccc gccgcccgt gggcctcct gacggcctg gaagcgccc gctgtgggc cgaagcgcg cgaaaattt gatggggag cgagcggaa tcggcgcaa tttggattt gatccagcg gtcgttgct ggtggtttg tctggatga ggggctggg gagtgggtt taactgtcg gttgcaacg cgggggcaa agggcacag tgccgggca cagggatga gaccaattt agccgtcca gatgataca cggaggtgg cagtacgtg cgatactgc caatcgatg gtttgataa aaccatcca gttgattgt gtctggaga tggtgcttg tggcttgtg ctacgagca ttcgcaagc cgaaggaat cgccgtgat acagcttgt ggagaagtt gtggaagca tgcagactc tcaaccagc tacctccga agtaccttc ggcttcgat aagccattt gccgccgcc cgagcgcct ggaatggac gctggacga caaggactt ttcccgcat cgaggaagc ggctctttc catcgacaa tttagtcaa agacttaga tttccaagt gttcagata tacgaatta cggcaagga ctttatcaa gtcctgcaa agtcagtcc agacgtcta catccaact cgcactaca actagctta ctatagact ctacggcaa attgaccgc gacctacga aagcgcctc aactcgcag attccgcct aggtcgagt ggactgcat ccggtccgc gagccccga ggccctggc ctgggccat ggccatggc ccagcccaa ggacgaaga cgacagcaa gaaagtaac cttccactt ggtcagcga cgaggagaa gctccggct gtggaagga ggccatcaa gcgccaaac caccgagat ggtggacaa tatcctggg ccaaggcat cgacatcca cctgctggg cttgcggga ggctgccag ggagacctc accgactgc tgcgcaccc actgccaga catcttcac cgattacac ttacaagtt agctaacaa atttctcct aagtacgag tcaagtcgc gacctcgac ggacagctt catggggta cggcccagt ggagccgga cgggtacgg agcctcgta taacccgaa acccgacca tattatttt ctgcttgtc ggcgttttg gtccagcga atcatgttt gtccgaaaa attccccgt aatgtttaa aaacacctt ccaaagtgg ctttctctc gattctgta cagtatcgg tagtaagcc gcttcagat ctgggataa gaaagtaaa aaacggcca cattaaaag gataaccga tgaggatat ccagtccct ggtcctgga agtggtcgg gtgtaatgt tagcaccac ttacatcac ctgccccgc cgaccccag gaagaccct aggaatcaa actccctta cttagtcat gattgtgaa aaatctcaa aaaatattt cacttttga agtccaaat tttagacga taagaacgt acgacggag gttcagggc tagcaatta tcagtcaac aacaagggt caagccgtt tatttgtac aatgccaat gaggctaga cgaaggctg gaaccaaat ccagttcaa tgtggctga ttttacacg acgtgccta tgggaccaa ctatgtgga gacgctaag ggtccaagt ccatgccaa ctgccgcat aagacgagt ctacttctc ggacagatt gtattcaga agacgaact tccagccga atttaaact gttcctacc aatccaaaa taagacacg aactgcggt tgcagcgca gtaaacatt ttaggaccg gacattagg tttttttgt tgtaatttg aacaaattg ctttttaaa ctcattagg taacacaaa attgtatgc aatcatcat tttagacac aataaactt catctttca ca
