Status
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Gene information
General information
TC032116
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000946,TC000947
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 3195957..3220643
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032116
    iBeetle screen iB_00194
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg condensed embryonic tissue, no cuticle, cns fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule, hours 100
    Adult morphology
    remarks: later than schedule, hours 100
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032116 RA
      MRRAKTLYD HGSARLALL AGPSPNRWR ESLLSLPKK 
      WLSGTSLVP PSSTTASTE ELPLPKLPV PELEQTMNK 
      YEYTMRPLL NTEEQRKLK NLIEKFAGP GGLGTQLQL 
      YLQDRREKM DNWAYDYWL DDMYLKNPS SLPVNSNPG 
      MVFPPRKFT TILDVARFT ARLLDAALD HKTYLDKKQ 
      LPVELATSR EPGQPLCMA QYYRILGTC RIPGRKKDA 
      QVVPGKSES EHVIVICRS QLYCVPVQA PDRGRLSED 
      EICAQLLHI LDDAPCLAS PPPVGLLTA WKRPLWAEA 
      RENLMGSER NRRNLDLIQ RSLLVVCLD EGLGSGFNC 
      RLQRGGKGH SAGHRDETN LAVQMIHGG GSTCDTANR 
      WFDKTIQLI VSGDGACGL CYEHSQAEG IAVIQLVEK 
      LWKHADSQP ATSEVPSAS ISHLPPPER LEWTLDDKD 
      FSRIEEAAL SIDNLVKDL DFQVFRYTN YGKDFIKSC 
      KVSPDVYIQ LALQLAYYR LYGKLTATY ESASTRRFR 
      LGRVDCIRS ASPEALAWA MAMAQPKDE DDSKKVTFH 
      LVSDEEKLR LWKEAIKRQ TTEMVDNIL GQGIDIHLL 
      GLREAARET SPTAAHPLP DIFTDYTYK LANKFLLST 
      SQVATSTDS FMGYGPVEP DGYGASYNP KPDHIIFCL 
      SAFWSSESC LSEKFPVMF KNTFQSGFL SILYSIGSK 
      PLQIWDKKV KNGHIKRIT DEDIQSLVL EVVGCNVST 
      TYITCPADP RKTLGIKLP YLVMIVKNL KKYFTFEVQ 
      ILDDKNVRR RFRASNYQS TTRVKPFIC TMPMRLDEG 
      WNQIQFNVA DFTRRAYGT NYVETLRVQ VHANCRIRR 
      VYFSDRLYS EDELPAEFK LFLPIQNKT RTAVAAQ
    CDS sequence
    • >TC032116 RA
      atgaggagg gctaaaacc ctctacgac cacggatca 
      gcgagattg gctttgtta gccggcccc agccccaac 
      cgatggcgc gaatcgctg ctttcgcta ccgaagaag 
      tggctgtcc gggacgtct ctggtgccc cccagctcg 
      accacggct agcactgag gagctcccc ctgcccaag 
      ctccccgtg ccggagtta gaacaaacc atgaacaag 
      tatgagtac acaatgaga ccgctcctg aacacggaa 
      gaacaaaga aagctgaaa aacctcatc gagaaattc 
      gctggacca ggcggactt gggacgcag ctacagctc 
      tatctgcag gatcggaga gagaaaatg gataattgg 
      gcttacgac tactggctc gacgacatg tacctaaaa 
      aacccatcc tccctccct gtaaactca aacccgggc 
      atggtattc ccgccccgg aaattcacc acaatcctg 
      gacgtggcc cgcttcacg gcccgcctt ctggacgca 
      gcactggac cacaaaacc tatctagac aagaagcaa 
      ctcccggtg gaactagca acgtcccgt gaacccggc 
      cagccgctt tgcatggcc cagtactac cgcattttg 
      ggcacttgc cggattccg ggccggaaa aaggacgca 
      caggtggtt ccgggcaag agcgagtcg gaacacgtg 
      atcgtaatc tgccggagc cagctgtac tgcgttccg 
      gtgcaggcc ccggaccgg ggccgcttg agcgaggac 
      gagatatgc gcccagttg ttgcacatt ttggacgac 
      gcgccttgt ctggccagc ccgccgccc gtgggcctc 
      ctgacggcc tggaagcgc ccgctgtgg gccgaagcg 
      cgcgaaaat ttgatgggg agcgagcgg aatcggcgc 
      aatttggat ttgatccag cggtcgttg ctggtggtt 
      tgtctggat gaggggctg gggagtggg tttaactgt 
      cggttgcaa cgcgggggc aaagggcac agtgccggg 
      cacagggat gagaccaat ttagccgtc cagatgata 
      cacggaggt ggcagtacg tgcgatact gccaatcga 
      tggtttgat aaaaccatc cagttgatt gtgtctgga 
      gatggtgct tgtggcttg tgctacgag cattcgcaa 
      gccgaagga atcgccgtg atacagctt gtggagaag 
      ttgtggaag catgcagac tctcaacca gctacctcc 
      gaagtacct tcggcttcg ataagccat ttgccgccg 
      cccgagcgc ctggaatgg acgctggac gacaaggac 
      ttttcccgc atcgaggaa gcggctctt tccatcgac 
      aatttagtc aaagactta gatttccaa gtgttcaga 
      tatacgaat tacggcaag gactttatc aagtcctgc 
      aaagtcagt ccagacgtc tacatccaa ctcgcacta 
      caactagct tactataga ctctacggc aaattgacc 
      gcgacctac gaaagcgcc tcaactcgc agattccgc 
      ctaggtcga gtggactgc atccggtcc gcgagcccc 
      gaggccctg gcctgggcc atggccatg gcccagccc 
      aaggacgaa gacgacagc aagaaagta accttccac 
      ttggtcagc gacgaggag aagctccgg ctgtggaag 
      gaggccatc aagcgccaa accaccgag atggtggac 
      aatatcctg ggccaaggc atcgacatc cacctgctg 
      ggcttgcgg gaggctgcc agggagacc tcaccgact 
      gctgcgcac ccactgcca gacatcttc accgattac 
      acttacaag ttagctaac aaatttctc ctaagtacg 
      agtcaagtc gcgacctcg acggacagc ttcatgggg 
      tacggccca gtggagccg gacgggtac ggagcctcg 
      tataacccg aaacccgac catattatt ttctgcttg 
      tcggcgttt tggtccagc gaatcatgt ttgtccgaa 
      aaattcccc gtaatgttt aaaaacacc ttccaaagt 
      ggctttctc tcgattctg tacagtatc ggtagtaag 
      ccgcttcag atctgggat aagaaagta aaaaacggc 
      cacattaaa aggataacc gatgaggat atccagtcc 
      ctggtcctg gaagtggtc gggtgtaat gttagcacc 
      acttacatc acctgcccc gccgacccc aggaagacc 
      ctaggaatc aaactccct tacttagtc atgattgtg 
      aaaaatctc aaaaaatat ttcactttt gaagtccaa 
      attttagac gataagaac gtacgacgg aggttcagg 
      gctagcaat tatcagtca acaacaagg gtcaagccg 
      tttatttgt acaatgcca atgaggcta gacgaaggc 
      tggaaccaa atccagttc aatgtggct gattttaca 
      cgacgtgcc tatgggacc aactatgtg gagacgcta 
      agggtccaa gtccatgcc aactgccgc ataagacga 
      gtctacttc tcggacaga ttgtattca gaagacgaa 
      cttccagcc gaatttaaa ctgttccta ccaatccaa 
      aataagaca cgaactgcg gttgcagcg cagtaa
    mRNA sequence
    • >TC032116 RA
      ggttgaatg aaaggagaa gtgcggcgc tttgtcgaa 
      cgagcgggt gccgagtca attgggcat gaggagggc 
      taaaaccct ctacgacca cggatcagc gagattggc 
      tttgttagc cggccccag ccccaaccg atggcgcga 
      atcgctgct ttcgctacc gaagaagtg gctgtccgg 
      gacgtctct ggtgccccc cagctcgac cacggctag 
      cactgagga gctccccct gcccaagct ccccgtgcc 
      ggagttaga acaaaccat gaacaagta tgagtacac 
      aatgagacc gctcctgaa cacggaaga acaaagaaa 
      gctgaaaaa cctcatcga gaaattcgc tggaccagg 
      cggacttgg gacgcagct acagctcta tctgcagga 
      tcggagaga gaaaatgga taattgggc ttacgacta 
      ctggctcga cgacatgta cctaaaaaa cccatcctc 
      cctccctgt aaactcaaa cccgggcat ggtattccc 
      gccccggaa attcaccac aatcctgga cgtggcccg 
      cttcacggc ccgccttct ggacgcagc actggacca 
      caaaaccta tctagacaa gaagcaact cccggtgga 
      actagcaac gtcccgtga acccggcca gccgctttg 
      catggccca gtactaccg cattttggg cacttgccg 
      gattccggg ccggaaaaa ggacgcaca ggtggttcc 
      gggcaagag cgagtcgga acacgtgat cgtaatctg 
      ccggagcca gctgtactg cgttccggt gcaggcccc 
      ggaccgggg ccgcttgag cgaggacga gatatgcgc 
      ccagttgtt gcacatttt ggacgacgc gccttgtct 
      ggccagccc gccgcccgt gggcctcct gacggcctg 
      gaagcgccc gctgtgggc cgaagcgcg cgaaaattt 
      gatggggag cgagcggaa tcggcgcaa tttggattt 
      gatccagcg gtcgttgct ggtggtttg tctggatga 
      ggggctggg gagtgggtt taactgtcg gttgcaacg 
      cgggggcaa agggcacag tgccgggca cagggatga 
      gaccaattt agccgtcca gatgataca cggaggtgg 
      cagtacgtg cgatactgc caatcgatg gtttgataa 
      aaccatcca gttgattgt gtctggaga tggtgcttg 
      tggcttgtg ctacgagca ttcgcaagc cgaaggaat 
      cgccgtgat acagcttgt ggagaagtt gtggaagca 
      tgcagactc tcaaccagc tacctccga agtaccttc 
      ggcttcgat aagccattt gccgccgcc cgagcgcct 
      ggaatggac gctggacga caaggactt ttcccgcat 
      cgaggaagc ggctctttc catcgacaa tttagtcaa 
      agacttaga tttccaagt gttcagata tacgaatta 
      cggcaagga ctttatcaa gtcctgcaa agtcagtcc 
      agacgtcta catccaact cgcactaca actagctta 
      ctatagact ctacggcaa attgaccgc gacctacga 
      aagcgcctc aactcgcag attccgcct aggtcgagt 
      ggactgcat ccggtccgc gagccccga ggccctggc 
      ctgggccat ggccatggc ccagcccaa ggacgaaga 
      cgacagcaa gaaagtaac cttccactt ggtcagcga 
      cgaggagaa gctccggct gtggaagga ggccatcaa 
      gcgccaaac caccgagat ggtggacaa tatcctggg 
      ccaaggcat cgacatcca cctgctggg cttgcggga 
      ggctgccag ggagacctc accgactgc tgcgcaccc 
      actgccaga catcttcac cgattacac ttacaagtt 
      agctaacaa atttctcct aagtacgag tcaagtcgc 
      gacctcgac ggacagctt catggggta cggcccagt 
      ggagccgga cgggtacgg agcctcgta taacccgaa 
      acccgacca tattatttt ctgcttgtc ggcgttttg 
      gtccagcga atcatgttt gtccgaaaa attccccgt 
      aatgtttaa aaacacctt ccaaagtgg ctttctctc 
      gattctgta cagtatcgg tagtaagcc gcttcagat 
      ctgggataa gaaagtaaa aaacggcca cattaaaag 
      gataaccga tgaggatat ccagtccct ggtcctgga 
      agtggtcgg gtgtaatgt tagcaccac ttacatcac 
      ctgccccgc cgaccccag gaagaccct aggaatcaa 
      actccctta cttagtcat gattgtgaa aaatctcaa 
      aaaatattt cacttttga agtccaaat tttagacga 
      taagaacgt acgacggag gttcagggc tagcaatta 
      tcagtcaac aacaagggt caagccgtt tatttgtac 
      aatgccaat gaggctaga cgaaggctg gaaccaaat 
      ccagttcaa tgtggctga ttttacacg acgtgccta 
      tgggaccaa ctatgtgga gacgctaag ggtccaagt 
      ccatgccaa ctgccgcat aagacgagt ctacttctc 
      ggacagatt gtattcaga agacgaact tccagccga 
      atttaaact gttcctacc aatccaaaa taagacacg 
      aactgcggt tgcagcgca gtaaacatt ttaggaccg 
      gacattagg tttttttgt tgtaatttg aacaaattg 
      ctttttaaa ctcattagg taacacaaa attgtatgc 
      aatcatcat tttagacac aataaactt catctttca 
      ca