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Gene information
General information
TC000941 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 8667779..8670803
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000941
    iBeetle screen iB_00193

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    remarks: later than schedule, hours 100
    Adult morphology
    remarks: later than schedule, hours 100
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000941 RA
      MLSLVSCLC FLPLIASSY KSPHFSPNR STIVHLFEW 
      KWSSIASEC ENFLQHKAY AGVQVSPPN ENLIIDGRP 
      WWERYQPVS YVLITRSGN ASSFADMVK RCNAVGVRI 
      YVDAVINHM SAGNGVGTG GSVANASHK FYPGVPYGP 
      DDFNKNCEI TNFDNNENA RNCELVGLK DLNQSKKYV 
      KGKIIEYLN HLIDLGVAG FRIDAARHM WPSDLREIY 
      SNLKNLNTE FGFAENLRP FIYQEVPTM DKEYTKLGA 
      LVEFNYGKS LSDVFRGND KLKWLVNWG PSWGLIDGS 
      DAVAFIDNH DTQRDKNGH ILSHKKAKQ YKMAVAFML 
      AHPYGPPAD KTGDLIPPN FNKDNTCGN GYVCEHRWR 
      EIYSMVQFR NVVAGTGVE NFWSNDDQQ ISFCRGNKG 
      FIVFTNWGD VKRDLQTCL PPGIYCDVI SGSLEAGNC 
      TGKAVRVKS DGKGFIRLG ALEFNGVLA IHEKSKLK
    CDS sequence
    • >TC000941 RA
      atgttaagc cttgtgtcg tgtctgtgt tttctcccc 
      ttgatagcc tcctcgtac aaatcgccc catttctcc 
      cccaaccgt tccaccatc gtccacctt ttcgaatgg 
      aaatggtcg agcatcgcc tccgaatgc gaaaacttc 
      ctccagcac aaagcctac gccggcgtt caggtttca 
      cccccgaac gaaaactta ataattgac ggccgacct 
      tggtgggaa agataccag cctgtgagc tacgtattg 
      attacgagg tccggtaac gcgagttct ttcgctgat 
      atggtcaag agatgcaac gcagtgggc gtgagaatt 
      tacgttgat gctgttatt aatcacatg tcggcggga 
      aatggggtc gggaccggt ggcagcgtg gcaaatgcc 
      tcgcataaa ttttaccca ggagtgcca tacggcccc 
      gatgatttc aataaaaat tgcgaaata accaacttt 
      gataataac gaaaatgca agaaactgc gaactggtc 
      ggtctgaaa gacttgaat cagagcaag aaatacgtg 
      aagggcaaa ataatcgaa tacttgaat catttaatc 
      gacctgggg gtggcgggg ttccgaatc gacgccgca 
      cgacacatg tggccctca gacttgaga gaaatctac 
      tcgaatttg aaaaatcta aacactgag tttggcttt 
      gccgaaaat ttgagaccg tttatttac caggaggtg 
      ccaacaatg gacaaggaa tatacaaaa ttgggagct 
      ttggtcgag ttcaattat gggaaatcg ttaagtgat 
      gtgtttaga ggcaatgat aaattaaaa tggttggtc 
      aattggggc ccatcgtgg ggtctaatc gatggttca 
      gacgctgtt gcgtttatt gacaaccac gacacccag 
      cgggacaaa aatgggcac attttgagc cacaaaaaa 
      gcaaagcag tacaagatg gcagttgcg tttatgttg 
      gcacaccca tatggtccc ccggctgat aagaccggt 
      gatttaatt cctccaaac tttaataag gataacact 
      tgtggcaat ggttacgtc tgcgagcat cgctggaga 
      gaaatctac agcatggtt caattccgg aacgtcgtt 
      gctgggacc ggtgttgaa aatttctgg tcgaacgat 
      gatcagcaa atatcgttc tgtcgcgga aataagggt 
      tttatagtt tttactaat tggggcgat gttaagcgg 
      gatttacag acgtgtcta cctcccggg atttactgt 
      gatgttata tcaggaagt ctggaggcc ggaaattgc 
      accgggaaa gccgttaga gtaaaaagt gacggcaaa 
      gggtttatt cggctgggg gcgcttgag tttaatggt 
      gtcctggcg atacacgaa aagtcaaaa ttgaaataa
    mRNA sequence
    • >TC000941 RA
      tctcacaag atgttaagc cttgtgtcg tgtctgtgt 
      tttctcccc ttgatagcc tcctcgtac aaatcgccc 
      catttctcc cccaaccgt tccaccatc gtccacctt 
      ttcgaatgg aaatggtcg agcatcgcc tccgaatgc 
      gaaaacttc ctccagcac aaagcctac gccggcgtt 
      caggtttca cccccgaac gaaaactta ataattgac 
      ggccgacct tggtgggaa agataccag cctgtgagc 
      tacgtattg attacgagg tccggtaac gcgagttct 
      ttcgctgat atggtcaag agatgcaac gcagtgggc 
      gtgagaatt tacgttgat gctgttatt aatcacatg 
      tcggcggga aatggggtc gggaccggt ggcagcgtg 
      gcaaatgcc tcgcataaa ttttaccca ggagtgcca 
      tacggcccc gatgatttc aataaaaat tgcgaaata 
      accaacttt gataataac gaaaatgca agaaactgc 
      gaactggtc ggtctgaaa gacttgaat cagagcaag 
      aaatacgtg aagggcaaa ataatcgaa tacttgaat 
      catttaatc gacctgggg gtggcgggg ttccgaatc 
      gacgccgca cgacacatg tggccctca gacttgaga 
      gaaatctac tcgaatttg aaaaatcta aacactgag 
      tttggcttt gccgaaaat ttgagaccg tttatttac 
      caggaggtg ccaacaatg gacaaggaa tatacaaaa 
      ttgggagct ttggtcgag ttcaattat gggaaatcg 
      ttaagtgat gtgtttaga ggcaatgat aaattaaaa 
      tggttggtc aattggggc ccatcgtgg ggtctaatc 
      gatggttca gacgctgtt gcgtttatt gacaaccac 
      gacacccag cgggacaaa aatgggcac attttgagc 
      cacaaaaaa gcaaagcag tacaagatg gcagttgcg 
      tttatgttg gcacaccca tatggtccc ccggctgat 
      aagaccggt gatttaatt cctccaaac tttaataag 
      gataacact tgtggcaat ggttacgtc tgcgagcat 
      cgctggaga gaaatctac agcatggtt caattccgg 
      aacgtcgtt gctgggacc ggtgttgaa aatttctgg 
      tcgaacgat gatcagcaa atatcgttc tgtcgcgga 
      aataagggt tttatagtt tttactaat tggggcgat 
      gttaagcgg gatttacag acgtgtcta cctcccggg 
      atttactgt gatgttata tcaggaagt ctggaggcc 
      ggaaattgc accgggaaa gccgttaga gtaaaaagt 
      gacggcaaa gggtttatt cggctgggg gcgcttgag 
      tttaatggt gtcctggcg atacacgaa aagtcaaaa 
      ttgaaataa tttagtcaa atgaatttt aataaaatt 
      gcctggcgc tgaagttct atacatacc aaatcaaca 
      caaacgttt ggattaaat cgaaaaata aattatttg 
      tccgctcca aaattgtaa aacaggaat aaataaaat 
      tattaatgc gccggcaag gtcaataca gccgaccat 
      aacgtgttt acgaaagga aaataaaac acaattaaa 
      tca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY