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Gene information
General information
TC000929 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 3105374..3110568
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000929
    iBeetle screen iB_00187
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    Adult morphology
    elytra shape rounded - Number of animals showing the phenotype: 6 (22 dpi)
    Days post injection



    hindwing potentially folding irregular - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection



    Fertility
    egg production: number no/almost no eggs -
    ovary size decreased - Number of animals showing the phenotype: 1

    vitellarium not present - Number of animals showing the phenotype: 1
    tropharium size decreased - Number of animals showing the phenotype: 1
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000929 RA
      MEAPPSPQS VGREFVRQY YTLLNKAPA HLHRFYNQN 
      SSFIHGGLD PPNRETSPV IGQKQIHQK IQQLNFHDC 
      HAKITQVDS QATLGSGVV VQVTGELSN AGQPMRRFT 
      QTFVLAAQS PKKYYVHND IFRYQDEII SDEECEPEN 
      RSEPEDETS QECPVLNDV QQMNQAPMN YYNPTNIAP 
      VPHHQPPHV LAPPAVPQV NGAVHPDDI NVMPGPVPH 
      VNAAGQGQV LPTPITTIQ PSTIVPPLN AAAPVEEAQ 
      NEVQNEEPQ EINYNEGNE QDNEQDLNQ EPVASNEPK 
      TYANLLKSG NSVPFNNPP LGTSPAQQP RSVSPPNMG 
      PRGEQGGLG NRNSMNRGP RVNQQRVQK QDSGRGAPG 
      RPSFNEEDD RKRPQGNSF GDQNQLFLG NLPHNATED 
      ELREIFSEF GSILDLRIH TKPANKVTL PNGRAPPNY 
      GFITYETQS GVQNCLAAK PIYYPKDDK NGTQLNVEE 
      KKTKDRQSY GSGRPSSND NRSRDSGPR RSGPGLGGA 
      NRNNATGGS QSGIIPNRT NNYNRGGGP PNRGPNTYN 
      RR
    CDS sequence
    • >TC000929 RA
      atggaggct cccccatct ccccaaagt gtaggaagg 
      gaattcgtt cgacaatac tacactttg ctgaacaag 
      gcccctgcc catctacac cgattttac aaccaaaac 
      tcctctttc atccatggc ggtttggac cctccaaac 
      cgggagacc agccctgtc atcggccag aaacaaatc 
      caccaaaaa atccagcag ctgaatttc cacgactgt 
      cacgctaag atcacccaa gtcgactcc caagccact 
      ctcggcagc ggtgtcgtc gtccaggtg accggcgag 
      ttatccaac gccggtcag ccgatgcgc cgtttcact 
      caaaccttc gtattggcc gcccaaagt cccaaaaag 
      tactacgtt cataatgac atcttcaga taccaggac 
      gagatcatc tccgacgag gaatgcgaa ccggaaaac 
      cgctccgaa ccggaagac gagacttcg caagagtgc 
      ccggttttg aacgacgtc cagcagatg aaccaagcc 
      cccatgaat tactacaac ccgactaac attgcgccg 
      gtgccccac caccagccc ccgcatgtt ctagcccct 
      ccggcggtg ccccaggtc aacggagcc gtgcatcct 
      gacgatatc aacgtgatg cccggaccg gtgccccat 
      gtcaacgcc gcgggccaa ggacaggtc cttccgaca 
      cccatcacc accatccaa ccttcgacg attgtacca 
      ccgttgaat gccgcggca ccggttgag gaagcccag 
      aacgaagtc caaaacgag gagccgcag gaaattaat 
      tacaatgaa gggaacgaa caggacaat gaacaggat 
      ttgaaccaa gagccggtt gcgtctaat gaacccaaa 
      acttatgcc aacttgttg aaatcgggc aatagcgta 
      ccgtttaat aacccacct ttgggtact tccccggcc 
      caacaacca cgatctgta tcaccgccg aatatgggg 
      cctagagga gaacaagga ggcctcgga aaccgcaac 
      agcatgaac cggggacct agagttaac caacaacga 
      gtccagaag caagattcg ggtcgcgga gccccaggc 
      cggccctct tttaacgaa gaagatgat aggaaacga 
      cctcaagga aacagtttc ggcgatcaa aaccagctg 
      ttcttgggc aatttgccc cacaatgcg accgaggac 
      gagctgcgc gagattttc tccgagttt ggttcaatt 
      ctcgacttg aggattcac acgaagccg gccaacaaa 
      gtaacactt cccaacggt cgcgctcca ccaaactac 
      ggtttcata acttatgag acgcaaagt ggtgtccag 
      aattgctta gccgcgaag ccgatttat tacccgaag 
      gatgacaag aacggcact cagttgaac gtggaggag 
      aagaagacg aaggatcgt cagtcgtac gggagcggt 
      cgtccgagc agcaacgat aaccgctcg cgcgactcc 
      gggcctcgt cggagcggc ccgggcctg gggggagcc 
      aataggaac aacgcaacg ggagggagt cagtcgggt 
      ataatcccg aatcggacc aataactac aacagaggc 
      gggggaccc ccgaaccga gggccgaac acttacaat 
      cgtcgctaa
    mRNA sequence
    • >TC000929 RA
      aaggtgtcc aaaatcgta actaaattg gcaatttat 
      gaagtttac acccgtggt tttgcggcg tttgtgcat 
      ttcaatcac atttctacc gtgttaata attaaattt 
      aaccgaaaa tacttcctt agctcgctt cgccgacta 
      tctagagga atggtaatg gaggctccc ccatctccc 
      caaagtgta ggaagggaa ttcgttcga caatactac 
      actttgctg aacaaggcc cctgcccat ctacaccga 
      ttttacaac caaaactcc tctttcatc catggcggt 
      ttggaccct ccaaaccgg gagaccagc cctgtcatc 
      ggccagaaa caaatccac caaaaaatc cagcagctg 
      aatttccac gactgtcac gctaagatc acccaagtc 
      gactcccaa gccactctc ggcagcggt gtcgtcgtc 
      caggtgacc ggcgagtta tccaacgcc ggtcagccg 
      atgcgccgt ttcactcaa accttcgta ttggccgcc 
      caaagtccc aaaaagtac tacgttcat aatgacatc 
      ttcagatac caggacgag atcatctcc gacgaggaa 
      tgcgaaccg gaaaaccgc tccgaaccg gaagacgag 
      acttcgcaa gagtgcccg gttttgaac gacgtccag 
      cagatgaac caagccccc atgaattac tacaacccg 
      actaacatt gcgccggtg ccccaccac cagcccccg 
      catgttcta gcccctccg gcggtgccc caggtcaac 
      ggagccgtg catcctgac gatatcaac gtgatgccc 
      ggaccggtg ccccatgtc aacgccgcg ggccaagga 
      caggtcctt ccgacaccc atcaccacc atccaacct 
      tcgacgatt gtaccaccg ttgaatgcc gcggcaccg 
      gttgaggaa gcccagaac gaagtccaa aacgaggag 
      ccgcaggaa attaattac aatgaaggg aacgaacag 
      gacaatgaa caggatttg aaccaagag ccggttgcg 
      tctaatgaa cccaaaact tatgccaac ttgttgaaa 
      tcgggcaat agcgtaccg tttaataac ccacctttg 
      ggtacttcc ccggcccaa caaccacga tctgtatca 
      ccgccgaat atggggcct agaggagaa caaggaggc 
      ctcggaaac cgcaacagc atgaaccgg ggacctaga 
      gttaaccaa caacgagtc cagaagcaa gattcgggt 
      cgcggagcc ccaggccgg ccctctttt aacgaagaa 
      gatgatagg aaacgacct caaggaaac agtttcggc 
      gatcaaaac cagctgttc ttgggcaat ttgccccac 
      aatgcgacc gaggacgag ctgcgcgag attttctcc 
      gagtttggt tcaattctc gacttgagg attcacacg 
      aagccggcc aacaaagta acacttccc aacggtcgc 
      gctccacca aactacggt ttcataact tatgagacg 
      caaagtggt gtccagaat tgcttagcc gcgaagccg 
      atttattac ccgaaggat gacaagaac ggcactcag 
      ttgaacgtg gaggagaag aagacgaag gatcgtcag 
      tcgtacggg agcggtcgt ccgagcagc aacgataac 
      cgctcgcgc gactccggg cctcgtcgg agcggcccg 
      ggcctgggg ggagccaat aggaacaac gcaacggga 
      gggagtcag tcgggtata atcccgaat cggaccaat 
      aactacaac agaggcggg ggacccccg aaccgaggg 
      ccgaacact tacaatcgt cgctaataa gtgaacttc 
      gcgcgaacg aattgtgaa tgggacgtg tacagtgtt 
      caagaacgg atcatgacg ttagtgcca atagtggtt 
      cattagctg catatttac atcaaacca tttcttttt 
      ttctcttgt ttaggattg ttgcgcaat gtccgaagc 
      gatatccgc caatcaata ctaatagtt ctctttttc 
      aagtactta ttggctgtg ttgtataat gcgattggt 
      tgatgaaaa aatcggtaa cgttcggag gttgagtaa 
      cgataaaaa caatgtttc gagcatagt tttatggtt 
      tgaacttaa atataagca ggtcaccac gggtcgtac 
      gttacaatt attttacat aacctaaca gctgtatgc 
      atgttgatg ttagtagct gctaactcg taactaaat 
      tttctacta gatacttat tttttattt tttaatgcc 
      aaatgtaca ttatatagg gagtgtaca taaataaaa 
      atttaaatt gaaaaaaat agtcttttt atgtgagct 
      gatattaac ggaatcagt agaaaattt agtgtattt 
      agcttctaa ggtcaccta ttcatatgg ttctaaaca 
      taatttagg attttgcgc tcgtatttt cagttactc 
      taagtattt atgttttta aggctcatt cggttcttt 
      ctcccgaaa attatttta ttcagttgt caacaccaa 
      gcgcaacgt aattactat gcacattag aaaaatttt 
      actttttgc agctaacca gttttggtt tgatcaaaa 
      ttttaaatc actcagccg tcatttttt gttgcgtac 
      tcttaaagt gtttgtgtc cacattaag ttaaagtta 
      catgtgaat aagtggagg gtgtaatcc ccattttat 
      ttaaggtag catgtgaaa gtgttttgt taattttta 
      taaatttgt ctggtgaaa aagtggaat gttatttac 
      aggcttagt ttcactttt ttttattcc ctaaagttt 
      tgttacagt atttgttcc ttttctcct gtgataatc 
      acatttcct ctcaaaagt atactcgac acagtttta 
      accaaagtt cttccttcg ctaaactaa gacttgtgc 
      aggttccac tagtcacaa atctacttt caggatctt 
      ttcagtttg tcgttcctg taatattgt caacttgtt 
      attagtaac attaaagga aat