General information | |||
TC000929 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 8611917..8617111 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC000929 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00187 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
embryo/egg - Penetrance (percentage of animals/eggs on slide): less than 30%
larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (22 dpi)
hindwing potentially folding irregular - Number of animals showing the phenotype: 3 (22 dpi)
ovary size decreased - Number of animals showing the phenotype: 1
vitellarium not present - Number of animals showing the phenotype: 1
tropharium size decreased - Number of animals showing the phenotype: 1
- >TC000929 RA
MEAPPSPQS VGREFVRQY YTLLNKAPA HLHRFYNQN SSFIHGGLD PPNRETSPV IGQKQIHQK IQQLNFHDC HAKITQVDS QATLGSGVV VQVTGELSN AGQPMRRFT QTFVLAAQS PKKYYVHND IFRYQDEII SDEECEPEN RSEPEDETS QECPVLNDV QQMNQAPMN YYNPTNIAP VPHHQPPHV LAPPAVPQV NGAVHPDDI NVMPGPVPH VNAAGQGQV LPTPITTIQ PSTIVPPLN AAAPVEEAQ NEVQNEEPQ EINYNEGNE QDNEQDLNQ EPVASNEPK TYANLLKSG NSVPFNNPP LGTSPAQQP RSVSPPNMG PRGEQGGLG NRNSMNRGP RVNQQRVQK QDSGRGAPG RPSFNEEDD RKRPQGNSF GDQNQLFLG NLPHNATED ELREIFSEF GSILDLRIH TKPANKVTL PNGRAPPNY GFITYETQS GVQNCLAAK PIYYPKDDK NGTQLNVEE KKTKDRQSY GSGRPSSND NRSRDSGPR RSGPGLGGA NRNNATGGS QSGIIPNRT NNYNRGGGP PNRGPNTYN RR
- >TC000929 RA
atggaggct cccccatct ccccaaagt gtaggaagg gaattcgtt cgacaatac tacactttg ctgaacaag gcccctgcc catctacac cgattttac aaccaaaac tcctctttc atccatggc ggtttggac cctccaaac cgggagacc agccctgtc atcggccag aaacaaatc caccaaaaa atccagcag ctgaatttc cacgactgt cacgctaag atcacccaa gtcgactcc caagccact ctcggcagc ggtgtcgtc gtccaggtg accggcgag ttatccaac gccggtcag ccgatgcgc cgtttcact caaaccttc gtattggcc gcccaaagt cccaaaaag tactacgtt cataatgac atcttcaga taccaggac gagatcatc tccgacgag gaatgcgaa ccggaaaac cgctccgaa ccggaagac gagacttcg caagagtgc ccggttttg aacgacgtc cagcagatg aaccaagcc cccatgaat tactacaac ccgactaac attgcgccg gtgccccac caccagccc ccgcatgtt ctagcccct ccggcggtg ccccaggtc aacggagcc gtgcatcct gacgatatc aacgtgatg cccggaccg gtgccccat gtcaacgcc gcgggccaa ggacaggtc cttccgaca cccatcacc accatccaa ccttcgacg attgtacca ccgttgaat gccgcggca ccggttgag gaagcccag aacgaagtc caaaacgag gagccgcag gaaattaat tacaatgaa gggaacgaa caggacaat gaacaggat ttgaaccaa gagccggtt gcgtctaat gaacccaaa acttatgcc aacttgttg aaatcgggc aatagcgta ccgtttaat aacccacct ttgggtact tccccggcc caacaacca cgatctgta tcaccgccg aatatgggg cctagagga gaacaagga ggcctcgga aaccgcaac agcatgaac cggggacct agagttaac caacaacga gtccagaag caagattcg ggtcgcgga gccccaggc cggccctct tttaacgaa gaagatgat aggaaacga cctcaagga aacagtttc ggcgatcaa aaccagctg ttcttgggc aatttgccc cacaatgcg accgaggac gagctgcgc gagattttc tccgagttt ggttcaatt ctcgacttg aggattcac acgaagccg gccaacaaa gtaacactt cccaacggt cgcgctcca ccaaactac ggtttcata acttatgag acgcaaagt ggtgtccag aattgctta gccgcgaag ccgatttat tacccgaag gatgacaag aacggcact cagttgaac gtggaggag aagaagacg aaggatcgt cagtcgtac gggagcggt cgtccgagc agcaacgat aaccgctcg cgcgactcc gggcctcgt cggagcggc ccgggcctg gggggagcc aataggaac aacgcaacg ggagggagt cagtcgggt ataatcccg aatcggacc aataactac aacagaggc gggggaccc ccgaaccga gggccgaac acttacaat cgtcgctaa
- >TC000929 RA
aaggtgtcc aaaatcgta actaaattg gcaatttat gaagtttac acccgtggt tttgcggcg tttgtgcat ttcaatcac atttctacc gtgttaata attaaattt aaccgaaaa tacttcctt agctcgctt cgccgacta tctagagga atggtaatg gaggctccc ccatctccc caaagtgta ggaagggaa ttcgttcga caatactac actttgctg aacaaggcc cctgcccat ctacaccga ttttacaac caaaactcc tctttcatc catggcggt ttggaccct ccaaaccgg gagaccagc cctgtcatc ggccagaaa caaatccac caaaaaatc cagcagctg aatttccac gactgtcac gctaagatc acccaagtc gactcccaa gccactctc ggcagcggt gtcgtcgtc caggtgacc ggcgagtta tccaacgcc ggtcagccg atgcgccgt ttcactcaa accttcgta ttggccgcc caaagtccc aaaaagtac tacgttcat aatgacatc ttcagatac caggacgag atcatctcc gacgaggaa tgcgaaccg gaaaaccgc tccgaaccg gaagacgag acttcgcaa gagtgcccg gttttgaac gacgtccag cagatgaac caagccccc atgaattac tacaacccg actaacatt gcgccggtg ccccaccac cagcccccg catgttcta gcccctccg gcggtgccc caggtcaac ggagccgtg catcctgac gatatcaac gtgatgccc ggaccggtg ccccatgtc aacgccgcg ggccaagga caggtcctt ccgacaccc atcaccacc atccaacct tcgacgatt gtaccaccg ttgaatgcc gcggcaccg gttgaggaa gcccagaac gaagtccaa aacgaggag ccgcaggaa attaattac aatgaaggg aacgaacag gacaatgaa caggatttg aaccaagag ccggttgcg tctaatgaa cccaaaact tatgccaac ttgttgaaa tcgggcaat agcgtaccg tttaataac ccacctttg ggtacttcc ccggcccaa caaccacga tctgtatca ccgccgaat atggggcct agaggagaa caaggaggc ctcggaaac cgcaacagc atgaaccgg ggacctaga gttaaccaa caacgagtc cagaagcaa gattcgggt cgcggagcc ccaggccgg ccctctttt aacgaagaa gatgatagg aaacgacct caaggaaac agtttcggc gatcaaaac cagctgttc ttgggcaat ttgccccac aatgcgacc gaggacgag ctgcgcgag attttctcc gagtttggt tcaattctc gacttgagg attcacacg aagccggcc aacaaagta acacttccc aacggtcgc gctccacca aactacggt ttcataact tatgagacg caaagtggt gtccagaat tgcttagcc gcgaagccg atttattac ccgaaggat gacaagaac ggcactcag ttgaacgtg gaggagaag aagacgaag gatcgtcag tcgtacggg agcggtcgt ccgagcagc aacgataac cgctcgcgc gactccggg cctcgtcgg agcggcccg ggcctgggg ggagccaat aggaacaac gcaacggga gggagtcag tcgggtata atcccgaat cggaccaat aactacaac agaggcggg ggacccccg aaccgaggg ccgaacact tacaatcgt cgctaataa gtgaacttc gcgcgaacg aattgtgaa tgggacgtg tacagtgtt caagaacgg atcatgacg ttagtgcca atagtggtt cattagctg catatttac atcaaacca tttcttttt ttctcttgt ttaggattg ttgcgcaat gtccgaagc gatatccgc caatcaata ctaatagtt ctctttttc aagtactta ttggctgtg ttgtataat gcgattggt tgatgaaaa aatcggtaa cgttcggag gttgagtaa cgataaaaa caatgtttc gagcatagt tttatggtt tgaacttaa atataagca ggtcaccac gggtcgtac gttacaatt attttacat aacctaaca gctgtatgc atgttgatg ttagtagct gctaactcg taactaaat tttctacta gatacttat tttttattt tttaatgcc aaatgtaca ttatatagg gagtgtaca taaataaaa atttaaatt gaaaaaaat agtcttttt atgtgagct gatattaac ggaatcagt agaaaattt agtgtattt agcttctaa ggtcaccta ttcatatgg ttctaaaca taatttagg attttgcgc tcgtatttt cagttactc taagtattt atgttttta aggctcatt cggttcttt ctcccgaaa attatttta ttcagttgt caacaccaa gcgcaacgt aattactat gcacattag aaaaatttt actttttgc agctaacca gttttggtt tgatcaaaa ttttaaatc actcagccg tcatttttt gttgcgtac tcttaaagt gtttgtgtc cacattaag ttaaagtta catgtgaat aagtggagg gtgtaatcc ccattttat ttaaggtag catgtgaaa gtgttttgt taattttta taaatttgt ctggtgaaa aagtggaat gttatttac aggcttagt ttcactttt ttttattcc ctaaagttt tgttacagt atttgttcc ttttctcct gtgataatc acatttcct ctcaaaagt atactcgac acagtttta accaaagtt cttccttcg ctaaactaa gacttgtgc aggttccac tagtcacaa atctacttt caggatctt ttcagtttg tcgttcctg taatattgt caacttgtt attagtaac attaaagga aat
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
