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Gene information
General information
TC000917 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 2918952..2941368
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000917
    iBeetle screen iB_00186
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (14 dpi)
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    Pupal morphology
    prothorax size decreased - Number of animals showing the phenotype: 7

    prothorax potentially transformed - Number of animals showing the phenotype: 7
    elytra duplicated - Number of animals showing the phenotype: 7

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000917 RA
      MSSYQFVNS LAQCYGQQA RDQQSSPGT DYYNPNANS 
      TYPPACYSP PQVAPQYPQ HPYAAPAPG HGLQPTMGD 
      YTQLQPQRL APTHLQSPN TQTHPSASC KYADSTSST 
      GVASPQDLS TNGAPPRST PPLSTPSNS NATKSSGLT 
      SPLSVSTSP PGKPATSTA SQNLSSPAS STSSTSSTE 
      KAGTNNNNS KSGQSSNPP QIYPWMKRV HLGQSTVNA 
      NGETKRQRT SYTRYQTLE LEKEFHFNR YLTRRRRIE 
      IAHALCLTE RQIKIWFQN RRMKWKKEH KMASMNIVP 
      YHMSPYGHP YQFDLHPSQ FAHLAT
    CDS sequence
    • >TC000917 RA
      atgagctcc taccagttc gtcaactcc ttggcccag 
      tgctacggc cagcaggcc cgcgaccag cagagcagt 
      cccgggaca gactattac aaccccaac gcgaactcc 
      acctacccc ccagcatgc tactcgccc ccgcaggtg 
      gccccccag tacccgcag cacccgtac gcggccccg 
      gcgccgggc cacggcctg cagcccacc atgggcgac 
      tacacccag ctgcagccg cagaggctg gccccgacc 
      cacctgcag agccccaac acccagacc cacccttcg 
      gccagttgc aagtacgcc gattcgacc tcctccaca 
      ggagtcgcc tccccgcaa gatctgagc acgaacgga 
      gctcctcca aggagtact ccgccgctt tcaaccccc 
      agtaacagc aacgcgacg aagagctcg ggactgacg 
      tccccgctg tcggtcagc acttcgcca ccaggcaaa 
      cccgcgacc tccacggca tcgcaaaac ttatcctcg 
      ccggcttct agcactagt tctacctct agtaccgaa 
      aaagccggc accaacaac aacaactcc aagagcggc 
      caatcgagc aacccgcct cagatctac ccctggatg 
      aagagagtt catctcggc caaagtact gtgaacgca 
      aatggcgaa acgaagcgg cagaggacg tcgtacacg 
      cgctatcag acgttagaa ttagaaaaa gaattccat 
      ttcaatcgg tacctgacg aggagacgg cgaatagaa 
      atagctcat gccttatgt ctgaccgaa agacaaata 
      aaaatctgg tttcaaaac cgccgcatg aagtggaaa 
      aaagagcac aagatggcc agtatgaat atcgttcct 
      tatcacatg tctccttac ggccaccct tatcagttt 
      gacttgcat cctagccaa tttgcccat ctcgctact 
      tag
    mRNA sequence
    • >TC000917 RA
      gaatgtcca tcacttacc cataaacca gcacgtgat 
      gtggtcgat ggtacttga cgcggtgtc attcggtgg 
      tacctgaag cagtgatag tgccctttc ggacccagt 
      gcgtgaatg ccacaatga gctcctacc agttcgtca 
      actccttgg cccagtgct acggccagc aggcccgcg 
      accagcaga gcagtcccg ggacagact attacaacc 
      ccaacgcga actccacct accccccag catgctact 
      cgcccccgc aggtggccc cccagtacc cgcagcacc 
      cgtacgcgg ccccggcgc cgggccacg gcctgcagc 
      ccaccatgg gcgactaca cccagctgc agccgcaga 
      ggctggccc cgacccacc tgcagagcc ccaacaccc 
      agacccacc cttcggcca gttgcaagt acgccgatt 
      cgacctcct ccacaggag tcgcctccc cgcaagatc 
      tgagcacga acggagctc ctccaagga gtactccgc 
      cgctttcaa cccccagta acagcaacg cgacgaaga 
      gctcgggac tgacgtccc cgctgtcgg tcagcactt 
      cgccaccag gcaaacccg cgacctcca cggcatcgc 
      aaaacttat cctcgccgg cttctagca ctagttcta 
      cctctagta ccgaaaaag ccggcacca acaacaaca 
      actccaaga gcggccaat cgagcaacc cgcctcaga 
      tctacccct ggatgaaga gagttcatc tcggccaaa 
      gtactgtga acgcaaatg gcgaaacga agcggcaga 
      ggacgtcgt acacgcgct atcagacgt tagaattag 
      aaaaagaat tccatttca atcggtacc tgacgagga 
      gacggcgaa tagaaatag ctcatgcct tatgtctga 
      ccgaaagac aaataaaaa tctggtttc aaaaccgcc 
      gcatgaagt ggaaaaaag agcacaaga tggccagta 
      tgaatatcg ttccttatc acatgtctc cttacggcc 
      acccttatc agtttgact tgcatccta gccaatttg 
      cccatctcg ctacttagg acgtgttta tgtgattcc 
      attagattt ccacctggc gtccctcca ggacgaagg 
      ctacgcatt ccaccaagc catggggat gggaggcat 
      gggtcccat gcagcgcgg tctttacgc ctgcggcag 
      cccttacag ctagccctg ggcctccta taacggaga 
      ctgcggccc ctcgtaggc cctttatcg gatctcagg 
      cggtctccg gcccacgac actcggacg aatttcaga 
      aaatcgtag acgtgtcgg ggtttgctt ttgtgccaa 
      tagacggtc actcggtat tggtgtacg tttccctca 
      agggtaggc ttgtactta tctctcgga cgagttttt 
      tctggacta gacgtgcaa tattgttaa atttttgtt 
      gatttttct ttgaaggga acttgtaag atatatcta 
      gaattttgt atctgaaaa ttgtgaaaa gacactgtg 
      ctcactata gttttaaaa caaagtgtg attaaacta 
      ggctaggac ttagtgtac atatacagc atgaaattc 
      acttttgta aataccgtc acgacttga agtaaatta 
      ttcctaagt taagtactg tatgaatgg ctctaggat 
      aatttgtaa ataaaacaa tatgtaaaa cacttatgt 
      atagacagt tcacttaaa agcttgata atctcaaga 
      aaaactctt tcggttcac taagtattg aagataagt 
      gtaaagttg tattatatt gtgtacatt ttagctcaa 
      cattgtttc attttcatt ttttagttt atttatgta 
      tttgtactt gtgtgtgtg aaagacgta caatttaga 
      ttgtacaaa atcctaata tttactgtt gtaattgtc 
      cactctgaa taaacaatt attaaaaaa aatcctctt 
      tttttaacc ttcaacgca cttgccaca aaagaccat 
      aaattgcat tcaaacaaa atgcaaagc aagaaaaca 
      cttggtggt caaacatca aaataaact cttctctgt 
      caatctttc cgtgtccat tttagacgt t