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Gene information
General information
TC000885 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 2275602..2288291
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000885
    iBeetle screen iB_00185
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands colour not present - Number of animals showing the phenotype: 6 (41 dpi)
    Days post injection



    abdominal odoriferous glands shape irregular - Number of animals showing the phenotype: 6 (41 dpi)
    Days post injection


    abdominal odoriferous glands structure irregular - Number of animals showing the phenotype: 6 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000885 RA
      MRQGWQETL LRGLLLLLL LLGSRSSVA SDSGNKDKN 
      LFGDKCTVD GDCEFVGAV CEVHGNGNG KCQCGPDVP 
      VTNHIDKCG EAVAVNKSC FFNEQCEAQ TPETECKDD 
      ICKCRFEKE PSTKPDGSV ECIVVQNAY YPKKYVDPA 
      MIGILVAMF LMFITICVV LRLFSRARW RENRTIFNT 
      PNPRLMNVS LLRENKLLH TDRRGSRSS ARGPSRQPS 
      MASLRAHSP NSQGRLFLG SRRGSRGSS NASATSNRS 
      NKSPPQAAN LSSATTPML ESVTVEVQE PKA
    • >TC000885 RB
      MRQGWQETL LRGLLLLLL LLGSRSSVA SDSGNKDKN 
      LFGDKCTVD GDCEFVGAV CEVHGNGNG KCQCGPDVP 
      VTNHIDKCG EAVAVNKSC FFNEQCEAQ TPETECKDD 
      ICKCRFEKE PSTKPDGSV ECIVVQNAY YPKKYVDPA 
      MIGILVAMF LMFITICVV LRLFSRARW RENRTIFNT 
      PNPRLMNVS LLRENKLLH TDRRGSRSS ARGPSRQPS 
      MASLRAHSP NSQGSRRGS RGSSNASAT SNRSNKSPP 
      QAANLSSAT TPMLESVTV EVQEPKA
    • >TC000885 RC
      MRQGWQETL LRGLLLLLL LLGSRSSVA SDSGNKDKN 
      LFGDKCTVD GDCEFVGAV CEVHGNGNG KCQCGPDVP 
      VTNHIDKCG EAVAVNKSC FFNEQCEAQ TPETECKDD 
      ICKCRFEKE PSTKPDGSV ECIVVQNAY YPKKYVDPA 
      MIGILVAMF LMFITICVV LRLFSRARW RENRTIFNT 
      PNPRLMNVS LLRENKLLH TDRRGSRSS ARGPSRQPS 
      MASLRAHSP NSQGIFSSS SSFLTNLQT CTIKML
    CDS sequence
    • >TC000885 RA
      atgaggcag ggctggcag gagacgctc ctgcggggg 
      ctactcctg ctcctgcta ctcctcgga tcccggagc 
      tccgtcgcg tcggattcc ggtaacaag gataagaac 
      ctttttgga gataaatgc acggtggac ggcgactgc 
      gagttcgtg ggagcagta tgcgaagtt cacggaaac 
      ggaaacggc aaatgtcag tgcggcccg gacgttcca 
      gttaccaac cacattgac aagtgtggc gaagccgtt 
      gcggtaaat aagtcctgt tttttcaat gagcaatgc 
      gaggctcaa actcccgag acggaatgc aaggacgat 
      atctgcaaa tgccggttc gaaaaggaa ccaagtacc 
      aagccagac gggtcggtg gaatgcatc gtcgtccaa 
      aatgcctac tacccgaaa aagtacgtc gatccggca 
      atgattggg atcttggtc gctatgttt ctcatgttt 
      attacaatt tgtgtcgtt ttgcgattg ttcagcagg 
      gcacgttgg agggagaac cgaaccata tttaacacg 
      cccaacccg cgcttgatg aacgtctct ctgcttcgt 
      gaaaacaag cttttacac acggaccgg cgagggtct 
      cggagcagt gccaggggc cccagcagg cagccaagt 
      atggcatct ctaagagct cactcccca aactctcaa 
      gggcgtttg tttctagga tctcgtcgc ggatcccga 
      ggcagcagc aacgcgtca gcgacttcg aaccgatcg 
      aacaagagt ccgccccaa gcggccaat ttgagttcg 
      gcgacgacg cccatgctg gagagcgtc accgtcgag 
      gtgcaagag cccaaagcg taa
    • >TC000885 RB
      atgaggcag ggctggcag gagacgctc ctgcggggg 
      ctactcctg ctcctgcta ctcctcgga tcccggagc 
      tccgtcgcg tcggattcc ggtaacaag gataagaac 
      ctttttgga gataaatgc acggtggac ggcgactgc 
      gagttcgtg ggagcagta tgcgaagtt cacggaaac 
      ggaaacggc aaatgtcag tgcggcccg gacgttcca 
      gttaccaac cacattgac aagtgtggc gaagccgtt 
      gcggtaaat aagtcctgt tttttcaat gagcaatgc 
      gaggctcaa actcccgag acggaatgc aaggacgat 
      atctgcaaa tgccggttc gaaaaggaa ccaagtacc 
      aagccagac gggtcggtg gaatgcatc gtcgtccaa 
      aatgcctac tacccgaaa aagtacgtc gatccggca 
      atgattggg atcttggtc gctatgttt ctcatgttt 
      attacaatt tgtgtcgtt ttgcgattg ttcagcagg 
      gcacgttgg agggagaac cgaaccata tttaacacg 
      cccaacccg cgcttgatg aacgtctct ctgcttcgt 
      gaaaacaag cttttacac acggaccgg cgagggtct 
      cggagcagt gccaggggc cccagcagg cagccaagt 
      atggcatct ctaagagct cactcccca aactctcaa 
      ggatctcgt cgcggatcc cgaggcagc agcaacgcg 
      tcagcgact tcgaaccga tcgaacaag agtccgccc 
      caagcggcc aatttgagt tcggcgacg acgcccatg 
      ctggagagc gtcaccgtc gaggtgcaa gagcccaaa 
      gcgtaa
    • >TC000885 RC
      atgaggcag ggctggcag gagacgctc ctgcggggg 
      ctactcctg ctcctgcta ctcctcgga tcccggagc 
      tccgtcgcg tcggattcc ggtaacaag gataagaac 
      ctttttgga gataaatgc acggtggac ggcgactgc 
      gagttcgtg ggagcagta tgcgaagtt cacggaaac 
      ggaaacggc aaatgtcag tgcggcccg gacgttcca 
      gttaccaac cacattgac aagtgtggc gaagccgtt 
      gcggtaaat aagtcctgt tttttcaat gagcaatgc 
      gaggctcaa actcccgag acggaatgc aaggacgat 
      atctgcaaa tgccggttc gaaaaggaa ccaagtacc 
      aagccagac gggtcggtg gaatgcatc gtcgtccaa 
      aatgcctac tacccgaaa aagtacgtc gatccggca 
      atgattggg atcttggtc gctatgttt ctcatgttt 
      attacaatt tgtgtcgtt ttgcgattg ttcagcagg 
      gcacgttgg agggagaac cgaaccata tttaacacg 
      cccaacccg cgcttgatg aacgtctct ctgcttcgt 
      gaaaacaag cttttacac acggaccgg cgagggtct 
      cggagcagt gccaggggc cccagcagg cagccaagt 
      atggcatct ctaagagct cactcccca aactctcaa 
      ggtatcttc tcttcttcc tcttccttt ctaactaat 
      cttcaaact tgtacaata aaaatgctg taa
    mRNA sequence
    • >TC000885 RA
      cagctgtac aacaaatgc actagaagt ctacccttc 
      aatcaaaca cccaactaa aaaaattag aaaaccaca 
      tttgcagga acaccatta tatcgcact ttcacagct 
      acatggctt cgtggggaa tagaaaatt tcaacgcaa 
      tctcccgaa actacaatt tcccatcag tgaaatatt 
      taagcgtat gctgagatg agttctcgc cccgagttg 
      aaattctct catcacata actttcgat caagttgtg 
      ataatccat tttgattga tactgttgt ttgtctggc 
      ggatgatga atggttgac actggccga attcctgca 
      gtaaaaaat cccgctata aagttttcc gccttcctg 
      ctggtcttc agcgaaccc ctgtaaatc cgaatggtg 
      ctcgcatct gtgcgctgc gcgtccgcg cccctgcag 
      gaaggcggc ggcggtggc agcaggaat cggcgactc 
      gtcagcggc ggcagtgcg ttcctgcat gatgagcgt 
      gagggccgc gtccgcgcc atgtgaaag tgttgagtg 
      accatgagg cagggctgg caggagacg ctcctgcgg 
      gggctactc ctgctcctg ctactcctc ggatcccgg 
      agctccgtc gcgtcggat tccggtaac aaggataag 
      aaccttttt ggagataaa tgcacggtg gacggcgac 
      tgcgagttc gtgggagca gtatgcgaa gttcacgga 
      aacggaaac ggcaaatgt cagtgcggc ccggacgtt 
      ccagttacc aaccacatt gacaagtgt ggcgaagcc 
      gttgcggta aataagtcc tgttttttc aatgagcaa 
      tgcgaggct caaactccc gagacggaa tgcaaggac 
      gatatctgc aaatgccgg ttcgaaaag gaaccaagt 
      accaagcca gacgggtcg gtggaatgc atcgtcgtc 
      caaaatgcc tactacccg aaaaagtac gtcgatccg 
      gcaatgatt gggatcttg gtcgctatg tttctcatg 
      tttattaca atttgtgtc gttttgcga ttgttcagc 
      agggcacgt tggagggag aaccgaacc atatttaac 
      acgcccaac ccgcgcttg atgaacgtc tctctgctt 
      cgtgaaaac aagctttta cacacggac cggcgaggg 
      tctcggagc agtgccagg ggccccagc aggcagcca 
      agtatggca tctctaaga gctcactcc ccaaactct 
      caagggcgt ttgtttcta ggatctcgt cgcggatcc 
      cgaggcagc agcaacgcg tcagcgact tcgaaccga 
      tcgaacaag agtccgccc caagcggcc aatttgagt 
      tcggcgacg acgcccatg ctggagagc gtcaccgtc 
      gaggtgcaa gagcccaaa gcgtaatca gcttcgcaa 
      tgttcgctc tttaagaat ctacaatct ctttactta 
      cttcggtag taatagtga tgttaggga ccaggaaag 
      gttaaattt gtccattca tcacgttca attcattag 
      tcaggttat ttattgtaa gatattatt ctgtaaagg 
      tttctttca agcacgggt ttctcaaac gttcaagca 
      cttatagag ttatttaga gagatatta ggagttaag 
      gttgatgat atatctaaa acttatctg ctttactta 
      atttatttc actaaatta agtttggtg gtacaaaaa 
      atactggga atattttgt gatactacc aaaatcaag 
      ctgtgattg tatgttggt agcttttag gcactgcgg 
      gtgacaaaa agtactggg aaaactttt tgccatttg 
      cagacttac actgtcatc gaattaggt tttattcaa 
      cattcttgt acagttcga aagcagaca tacagtaca 
      gttcatgct cattgagaa tatatacct ctcgtaggt 
      tcttcttca aaaacaaat agaaatttt attatttta 
      ggagaatgt agatttagg ttccaatct agatgtgag 
      aagctactc ggttgtaat cgtagacta agatttatt 
      cattgatga tatattgat attacacac gagcttagc 
      tctgcttgc tttaaaata taatacgag ttgaattat 
      tataaaact aatgtatat taaagcatt tttatcaat 
      ctattataa gcttatatc tttatactc ttgtactgt 
      atactgtat tttcacgac tatatccta tatacttgc 
      caatacttt taataaaac gctattaaa gac
    • >TC000885 RB
      cagctgtac aacaaatgc actagaagt ctacccttc 
      aatcaaaca cccaactaa aaaaattag aaaaccaca 
      tttgcagga acaccatta tatcgcact ttcacagct 
      acatggctt cgtggggaa tagaaaatt tcaacgcaa 
      tctcccgaa actacaatt tcccatcag tgaaatatt 
      taagcgtat gctgagatg agttctcgc cccgagttg 
      aaattctct catcacata actttcgat caagttgtg 
      ataatccat tttgattga tactgttgt ttgtctggc 
      ggatgatga atggttgac actggccga attcctgca 
      gtaaaaaat cccgctata aagttttcc gccttcctg 
      ctggtcttc agcgaaccc ctgtaaatc cgaatggtg 
      ctcgcatct gtgcgctgc gcgtccgcg cccctgcag 
      gaaggcggc ggcggtggc agcaggaat cggcgactc 
      gtcagcggc ggcagtgcg ttcctgcat gatgagcgt 
      gagggccgc gtccgcgcc atgtgaaag tgttgagtg 
      accatgagg cagggctgg caggagacg ctcctgcgg 
      gggctactc ctgctcctg ctactcctc ggatcccgg 
      agctccgtc gcgtcggat tccggtaac aaggataag 
      aaccttttt ggagataaa tgcacggtg gacggcgac 
      tgcgagttc gtgggagca gtatgcgaa gttcacgga 
      aacggaaac ggcaaatgt cagtgcggc ccggacgtt 
      ccagttacc aaccacatt gacaagtgt ggcgaagcc 
      gttgcggta aataagtcc tgttttttc aatgagcaa 
      tgcgaggct caaactccc gagacggaa tgcaaggac 
      gatatctgc aaatgccgg ttcgaaaag gaaccaagt 
      accaagcca gacgggtcg gtggaatgc atcgtcgtc 
      caaaatgcc tactacccg aaaaagtac gtcgatccg 
      gcaatgatt gggatcttg gtcgctatg tttctcatg 
      tttattaca atttgtgtc gttttgcga ttgttcagc 
      agggcacgt tggagggag aaccgaacc atatttaac 
      acgcccaac ccgcgcttg atgaacgtc tctctgctt 
      cgtgaaaac aagctttta cacacggac cggcgaggg 
      tctcggagc agtgccagg ggccccagc aggcagcca 
      agtatggca tctctaaga gctcactcc ccaaactct 
      caaggatct cgtcgcgga tcccgaggc agcagcaac 
      gcgtcagcg acttcgaac cgatcgaac aagagtccg 
      ccccaagcg gccaatttg agttcggcg acgacgccc 
      atgctggag agcgtcacc gtcgaggtg caagagccc 
      aaagcgtaa tcagcttcg caatgttcg ctctttaag 
      aatctacaa tctctttac ttacttcgg tagtaatag 
      tgatgttag ggaccagga aaggttaaa tttgtccat 
      tcatcacgt tcaattcat tagtcaggt tatttattg 
      taagatatt attctgtaa aggtttctt tcaagcacg 
      ggtttctca aacgttcaa gcacttata gagttattt 
      agagagata ttaggagtt aaggttgat gatatatct 
      aaaacttat ctgctttac ttaatttat ttcactaaa 
      ttaagtttg gtggtacaa aaaatactg ggaatattt 
      tgtgatact accaaaatc aagctgtga ttgtatgtt 
      ggtagcttt taggcactg cgggtgaca aaaagtact 
      gggaaaact ttttgccat ttgcagact tacactgtc 
      atcgaatta ggttttatt caacattct tgtacagtt 
      cgaaagcag acatacagt acagttcat gctcattga 
      gaatatata cctctcgta ggttcttct tcaaaaaca 
      aatagaaat tttattatt ttaggagaa tgtagattt 
      aggttccaa tctagatgt gagaagcta ctcggttgt 
      aatcgtaga ctaagattt attcattga tgatatatt 
      gatattaca cacgagctt agctctgct tgctttaaa 
      atataatac gagttgaat tattataaa actaatgta 
      tattaaagc atttttatc aatctatta taagcttat 
      atctttata ctcttgtac tgtatactg tattttcac 
      gactatatc ctatatact tgccaatac ttttaataa 
      aacgctatt aaagac
    • >TC000885 RC
      cagctgtac aacaaatgc actagaagt ctacccttc 
      aatcaaaca cccaactaa aaaaattag aaaaccaca 
      tttgcagga acaccatta tatcgcact ttcacagct 
      acatggctt cgtggggaa tagaaaatt tcaacgcaa 
      tctcccgaa actacaatt tcccatcag tgaaatatt 
      taagcgtat gctgagatg agttctcgc cccgagttg 
      aaattctct catcacata actttcgat caagttgtg 
      ataatccat tttgattga tactgttgt ttgtctggc 
      ggatgatga atggttgac actggccga attcctgca 
      gtaaaaaat cccgctata aagttttcc gccttcctg 
      ctggtcttc agcgaaccc ctgtaaatc cgaatggtg 
      ctcgcatct gtgcgctgc gcgtccgcg cccctgcag 
      gaaggcggc ggcggtggc agcaggaat cggcgactc 
      gtcagcggc ggcagtgcg ttcctgcat gatgagcgt 
      gagggccgc gtccgcgcc atgtgaaag tgttgagtg 
      accatgagg cagggctgg caggagacg ctcctgcgg 
      gggctactc ctgctcctg ctactcctc ggatcccgg 
      agctccgtc gcgtcggat tccggtaac aaggataag 
      aaccttttt ggagataaa tgcacggtg gacggcgac 
      tgcgagttc gtgggagca gtatgcgaa gttcacgga 
      aacggaaac ggcaaatgt cagtgcggc ccggacgtt 
      ccagttacc aaccacatt gacaagtgt ggcgaagcc 
      gttgcggta aataagtcc tgttttttc aatgagcaa 
      tgcgaggct caaactccc gagacggaa tgcaaggac 
      gatatctgc aaatgccgg ttcgaaaag gaaccaagt 
      accaagcca gacgggtcg gtggaatgc atcgtcgtc 
      caaaatgcc tactacccg aaaaagtac gtcgatccg 
      gcaatgatt gggatcttg gtcgctatg tttctcatg 
      tttattaca atttgtgtc gttttgcga ttgttcagc 
      agggcacgt tggagggag aaccgaacc atatttaac 
      acgcccaac ccgcgcttg atgaacgtc tctctgctt 
      cgtgaaaac aagctttta cacacggac cggcgaggg 
      tctcggagc agtgccagg ggccccagc aggcagcca 
      agtatggca tctctaaga gctcactcc ccaaactct 
      caaggtatc ttctcttct tcctcttcc tttctaact 
      aatcttcaa acttgtaca ataaaaatg ctgtaaata 
      tgtgaattt gccgattaa tctgatgag cgctgcctt 
      tgtttaatg ccttctggg acacgatag aaggttatt 
      agttttggc tactttgca ctttgcacc tcttgcttt 
      cgcatcttt gcatttctg tgtgtgaat acggtagag 
      agtagagaa tcaccaggt cctgctgca ctgcctgat 
      gaatcaccg ggaccctac gacagggcg tttgtttct 
      aggatctcg tcgcggatc ccgaggcag cagcaacgc 
      gtcagcgac ttcgaaccg atcgaacaa gagtccgcc 
      ccaagcggc caatttgag ttcggcgac gacgcccat 
      gctggagag cgtcaccgt cgaggtgca agagcccaa 
      agcgtaatc agcttcgca atgttcgct ctttaagaa 
      tctacaatc tctttactt acttcggta gtaatagtg 
      atgttaggg accaggaaa ggttaaatt tgtccattc 
      atcacgttc aattcatta gtcaggtta tttattgta 
      agatattat tctgtaaag gtttctttc aagcacggg 
      tttctcaaa cgttcaagc acttataga gttatttag 
      agagatatt aggagttaa ggttgatga tatatctaa 
      aacttatct gctttactt aatttattt cactaaatt 
      aagtttggt ggtacaaaa aatactggg aatattttg 
      tgatactac caaaatcaa gctgtgatt gtatgttgg 
      tagctttta ggcactgcg ggtgacaaa aagtactgg 
      gaaaacttt ttgccattt gcagactta cactgtcat 
      cgaattagg ttttattca acattcttg tacagttcg 
      aaagcagac atacagtac agttcatgc tcattgaga 
      atatatacc tctcgtagg ttcttcttc aaaaacaaa 
      tagaaattt tattatttt aggagaatg tagatttag 
      gttccaatc tagatgtga gaagctact cggttgtaa 
      tcgtagact aagatttat tcattgatg atatattga 
      tattacaca cgagcttag ctctgcttg ctttaaaat 
      ataatacga gttgaatta ttataaaac taatgtata 
      ttaaagcat ttttatcaa tctattata agcttatat 
      ctttatact cttgtactg tatactgta ttttcacga 
      ctatatcct atatacttg ccaatactt ttaataaaa 
      cgctattaa agac