Status
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Gene information
General information
TC000880 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 7743071..7751589
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000880
    iBeetle screen iB_00184

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature partially randomrounded - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000880 RA
      MLGYTSYRD WSPAGATAQ QQLSVVTTV WGVTTSTQS 
      GPHGGYSGG PTPAGNPNY GNTLKQQQT SYQTAYRQT 
      GPGVGNYGG GNGMNGSEG GYGGGNALS TAAMVAAAT 
      ATATATASV VALQDNSQF STQQYSQGY QQRMMPMNG 
      MSSMNGMNT MNPMSNMAN MGMGNMHNN MMGNMGPKM 
      GMQSPGPTN NSMYPRRMT PYPSPAMHM SQKRGQQYT 
      NSGPCPTMN PNFNPNGQY YGTRQPNFN QYPSQQTLG 
      PSGNFGPAG MGPVVRGAA ALRQTTPPY TNQGQYFPN 
      GPMNQFSND SQYMTRNGQ YGNTNQFQQ DVAMRGNMN 
      YQHSPIPGN PTPPLTPAS SMPPYISPN PDVKPNFNE 
      MKPIIPSQK DDELRLTFP VRDGIILPP FRLEHNLAV 
      SNHVFQLKP TVHQTLMWR SDLELQLKC FHHEDRQMN 
      TNWPASVQV SVNATPLII DRGENKTSH KPLYLKDVC 
      QPGRNTIQI TVSACCCSH LFVLQLVHR PSVRSVLQG 
      LLRKRLLTA DHCIAKIKR NFGNTNSGP NMDRDRDPV 
      EQTALKVSL KCPITYKRI TLPARGHDC KHIQCFDLE 
      SYLQMNCER GAWRCPVCN KPAQLEGLE VDQYMWGIL 
      NTLSNSEVD EVTIDSSAN WKAAKGLGA TIKMEDDND 
      SCGAKRLNK AMSPGSMTL PTMNNWDMS QAMSPYIPP 
      DMNSIASGS MMNGPPSYN NTRNGQYDF GSSTNSGNN 
      EYSAGNGPL SHLSDSVNS LDPLNAMEK SLNEQMPHT 
      PHTPHTPHT PHTPGGTPG SAHTPGGTG PPSVPPATD 
      HHNNSNSSN SSSNNNSGN NEAVPEIPS DLNFDPAAV 
      IDGEGTGQE ALNLLPDSV DPMELLSYL DPPDLNTPP 
      SSGSSAGHA NTSASDDLL ALFE
    CDS sequence
    • >TC000880 RA
      atgctggga tatacttcc tacagggac tggtcgcca 
      gcgggcgcc acggcccag cagcagttg tcagtcgtg 
      accacggtg tggggagtc accacgtct actcagtcg 
      ggcccccac ggaggctac tcgggtggc ccgacacct 
      gcaggaaat cccaactac ggaaacacc ctcaaacag 
      cagcagact tcctatcaa acggcctac aggcagacc 
      gggcctggg gttggaaat tatggtggt ggtaatgga 
      atgaacggt agtgaggga ggttacgga ggtggtaat 
      gccctgtca acagctgcg atggtagcg gctgcgaca 
      gcaacggcc accgccacc gcgagcgtg gtggcactc 
      caagacaac agtcaattc tccacccag cagtattct 
      cagggttat cagcaacgc atgatgccc atgaacggg 
      atgagcagc atgaacggc atgaacacc atgaacccc 
      atgtcgaac atggccaat atgggcatg ggcaatatg 
      cacaacaac atgatgggc aacatgggc ccgaaaatg 
      ggcatgcaa tcgccgggg cctaccaac aactccatg 
      tacccgcgc cgaatgacc ccatacccg tcccccgcc 
      atgcacatg tcgcagaaa cgggggcag cagtacacc 
      aactcgggc ccttgcccc accatgaac cccaacttc 
      aaccccaac ggccagtac tatgggacg cggcagccc 
      aacttcaac cagtacccc tcccagcag acgctgggg 
      cccagcggg aacttcggc ccggcgggg atggggccc 
      gtggtgcgg ggggcggcg gcgctgcgg caaaccacc 
      ccgccgtac accaaccaa ggccagtat ttccccaac 
      ggacccatg aaccagttt tcgaatgac tcgcagtac 
      atgacgcgg aatgggcaa tatggcaat accaaccag 
      ttccagcaa gatgtggcc atgaggggc aacatgaac 
      taccagcac agccccatc ccgggcaac cccaccccg 
      ccgctcacc cccgcgagt agcatgcca ccctacatt 
      agccccaat ccggacgtc aagcccaac ttcaacgaa 
      atgaaaccc ataattccc agtcaaaaa gatgacgag 
      ctccggtta actttcccc gtccgagac ggcatcatt 
      ttgcccccc tttcggtta gaacacaat ctcgcggtt 
      agcaatcac gtttttcag ttaaaaccg acggtccat 
      cagacttta atgtggcgt tctgactta gaattacaa 
      ctcaagtgc ttccaccac gaagataga caaatgaac 
      acgaattgg ccggcttcc gtgcaagtc tcagtaaac 
      gcaacacct ctcattata gaccggggc gagaacaag 
      acatcgcat aaacctctg taccttaaa gatgtctgc 
      cagcccgga cgaaacact atccaaatc acagtttcg 
      gcttgttgt tgttcgcac ctcttcgtc ctacaactt 
      gtacataga cccagtgtt agatcagtg cttcaaggc 
      ctcctccgg aaacggctc cttacggcc gatcattgt 
      atagcgaaa attaaacga aacttcggc aataccaac 
      tctggcccg aacatggac cgagaccgg gatcctgtc 
      gaacaaacc gctctcaaa gtctccctc aagtgtcca 
      atcacctac aagaggata acactgcca gcgagagga 
      cacgactgt aaacacatc cagtgcttc gatttggag 
      tcttacctc caaatgaac tgcgagagg ggcgcttgg 
      aggtgtccg gtctgtaat aagccagcg cagctggaa 
      ggcctggaa gtagatcag tacatgtgg ggaattttg 
      aacacgtta agcaacagc gaagtcgac gaagtcacg 
      attgacagc tcggccaac tggaaggcg gccaaaggc 
      ctgggcgcg actatcaag atggaggac gataatgac 
      tcctgtgga gccaaacgg ctgaacaag gcaatgtct 
      ccgggtagt atgacgctg ccgacgatg aacaactgg 
      gacatgtcg caagcaatg tccccctat atacctccc 
      gacatgaac agtattgca agcgggtcg atgatgaac 
      ggccctcct tcctacaac aacactcgg aacggtcag 
      tacgacttc ggatcgtca accaactca ggaaacaat 
      gagtattcg gccggaaat ggccccctg tcccatctg 
      agcgattcg gtcaattcg ctcgatccg ttaaacgcc 
      atggagaag agtttgaac gaacagatg ccacacacg 
      ccccatacg ccgcacacc cctcacacg ccgcacact 
      cccggagga acgcccgga tcggcacac actccggga 
      gggaccggc ccccctagt gttcctcca gcgacggac 
      caccacaac aatagcaac agttcaaat agtagtagt 
      aataataac agtgggaac aacgaggcc gttcccgaa 
      atcccctca gacttgaac tttgaccca gctgcggtt 
      attgacggt gaaggcaca ggccaggaa gcacttaat 
      ttgttgccg gacagcgtg gatccgatg gagttgttg 
      tcgtacttg gaccctcct gacctgaat acgccccca 
      tctagtggt agcagtgca gggcatgcc aacacctcg 
      gcgagtgat gacctcctt gctctgttc gaataa
    mRNA sequence
    • >TC000880 RA
      cgttgtttc tctcacagg cagacagtt gaacgtcgc 
      gttccggtt gtgtagtac gcgcgcgtt aaaacgctc 
      gctccggta aaactgtgt taacaaaat attatggtc 
      gtgtaaaag tgtctcaaa cattgacta tctttatgt 
      ttaccctca aatgctggg atatacttc ctacaggga 
      ctggtcgcc agcgggcgc cacggccca gcagcagtt 
      gtcagtcgt gaccacggt gtggggagt caccacgtc 
      tactcagtc gggccccca cggaggcta ctcgggtgg 
      cccgacacc tgcaggaaa tcccaacta cggaaacac 
      cctcaaaca gcagcagac ttcctatca aacggccta 
      caggcagac cgggcctgg ggttggaaa ttatggtgg 
      tggtaatgg aatgaacgg tagtgaggg aggttacgg 
      aggtggtaa tgccctgtc aacagctgc gatggtagc 
      ggctgcgac agcaacggc caccgccac cgcgagcgt 
      ggtggcact ccaagacaa cagtcaatt ctccaccca 
      gcagtattc tcagggtta tcagcaacg catgatgcc 
      catgaacgg gatgagcag catgaacgg catgaacac 
      catgaaccc catgtcgaa catggccaa tatgggcat 
      gggcaatat gcacaacaa catgatggg caacatggg 
      cccgaaaat gggcatgca atcgccggg gcctaccaa 
      caactccat gtacccgcg ccgaatgac cccataccc 
      gtcccccgc catgcacat gtcgcagaa acgggggca 
      gcagtacac caactcggg cccttgccc caccatgaa 
      ccccaactt caaccccaa cggccagta ctatgggac 
      gcggcagcc caacttcaa ccagtaccc ctcccagca 
      gacgctggg gcccagcgg gaacttcgg cccggcggg 
      gatggggcc cgtggtgcg gggggcggc ggcgctgcg 
      gcaaaccac cccgccgta caccaacca aggccagta 
      tttccccaa cggacccat gaaccagtt ttcgaatga 
      ctcgcagta catgacgcg gaatgggca atatggcaa 
      taccaacca gttccagca agatgtggc catgagggg 
      caacatgaa ctaccagca cagccccat cccgggcaa 
      ccccacccc gccgctcac ccccgcgag tagcatgcc 
      accctacat tagccccaa tccggacgt caagcccaa 
      cttcaacga aatgaaacc cataattcc cagtcaaaa 
      agatgacga gctccggtt aactttccc cgtccgaga 
      cggcatcat tttgccccc ctttcggtt agaacacaa 
      tctcgcggt tagcaatca cgtttttca gttaaaacc 
      gacggtcca tcagacttt aatgtggcg ttctgactt 
      agaattaca actcaagtg cttccacca cgaagatag 
      acaaatgaa cacgaattg gccggcttc cgtgcaagt 
      ctcagtaaa cgcaacacc tctcattat agaccgggg 
      cgagaacaa gacatcgca taaacctct gtaccttaa 
      agatgtctg ccagcccgg acgaaacac tatccaaat 
      cacagtttc ggcttgttg ttgttcgca cctcttcgt 
      cctacaact tgtacatag acccagtgt tagatcagt 
      gcttcaagg cctcctccg gaaacggct ccttacggc 
      cgatcattg tatagcgaa aattaaacg aaacttcgg 
      caataccaa ctctggccc gaacatgga ccgagaccg 
      ggatcctgt cgaacaaac cgctctcaa agtctccct 
      caagtgtcc aatcaccta caagaggat aacactgcc 
      agcgagagg acacgactg taaacacat ccagtgctt 
      cgatttgga gtcttacct ccaaatgaa ctgcgagag 
      gggcgcttg gaggtgtcc ggtctgtaa taagccagc 
      gcagctgga aggcctgga agtagatca gtacatgtg 
      gggaatttt gaacacgtt aagcaacag cgaagtcga 
      cgaagtcac gattgacag ctcggccaa ctggaaggc 
      ggccaaagg cctgggcgc gactatcaa gatggagga 
      cgataatga ctcctgtgg agccaaacg gctgaacaa 
      ggcaatgtc tccgggtag tatgacgct gccgacgat 
      gaacaactg ggacatgtc gcaagcaat gtcccccta 
      tatacctcc cgacatgaa cagtattgc aagcgggtc 
      gatgatgaa cggccctcc ttcctacaa caacactcg 
      gaacggtca gtacgactt cggatcgtc aaccaactc 
      aggaaacaa tgagtattc ggccggaaa tggccccct 
      gtcccatct gagcgattc ggtcaattc gctcgatcc 
      gttaaacgc catggagaa gagtttgaa cgaacagat 
      gccacacac gccccatac gccgcacac ccctcacac 
      gccgcacac tcccggagg aacgcccgg atcggcaca 
      cactccggg agggaccgg cccccctag tgttcctcc 
      agcgacgga ccaccacaa caatagcaa cagttcaaa 
      tagtagtag taataataa cagtgggaa caacgaggc 
      cgttcccga aatcccctc agacttgaa ctttgaccc 
      agctgcggt tattgacgg tgaaggcac aggccagga 
      agcacttaa tttgttgcc ggacagcgt ggatccgat 
      ggagttgtt gtcgtactt ggaccctcc tgacctgaa 
      tacgccccc atctagtgg tagcagtgc agggcatgc 
      caacacctc ggcgagtga tgacctcct tgctctgtt 
      cgaataaga tgagttcct gagctcacg accacgtct 
      cgtggtgga catgtgagt gaacaaagt gtgataaaa 
      caatctaaa cgtgttaat tattgaagt aaaggtcta 
      aatcaaact attttcact gtattattt gtacataac 
      tacttaaaa gaatggcgt gagttttta tttcattgg 
      ttggataaa gtctattga gatatattt aaattattg 
      agaaggtct attttgatt ttaatgcca aaattttat 
      tgtattata tgagagacc agtggaata aaaagcgac 
      tcgtgacgg ccagtttag tataatatt attaactac 
      atgaatata cttactata gtatagaag ttggaacca 
      aacgatcta gtacttcta gaaacggcc gagttgtcg 
      gtctttttg catctcgat gtgcatagg cttcaagta 
      ttatctgct gtattcgat tcagtgaac ctaatataa 
      cgcagacaa cacgtgtaa cgattccac acaaccaca 
      tgacagtag aaatttcaa attgtattt tgattgtat 
      tatttgttt caggcttaa atatctgca caagcatcc 
      acatataat attattatt aattgagaa ctctgagac 
      tgtgataaa actttaatt ataagtaat tttaacata 
      tttgataat tatatattt ataatcgtg gttctttag 
      cgtcgtttg aatttgtat taaacaaaa atattatgt 
      tatagtgtg gctgctgtt tgtgtgtgt tttcactaa 
      agttaattt gatgtgata aaatatatt ttggttaaa 
      tacaatttg agttttatt cccaaatcc ttatacgtg 
      tttgtcact tgtattgat ttaagtagg gtccaagtt 
      cctaaaagc aaaaatgta ttcactttg tattgatac 
      aaattatat caggttcac tcgaattgc gcaattctt 
      attgccaag aataactca ttctaaaag gtaggcaga 
      atagatgtc aataagttg tatgtaact agccaaagt 
      agagaattt ttatcaaat tattgaaag tttacaggc 
      acatgacaa catacaaaa gtttatcca atcacgcgg 
      tgtaaactt ttaataagt tcagtataa attttgcca 
      ataatttat tgtttttca ttagcttca ttttcagaa 
      aagggactt ttgtaaatt attttcgac aatggacca 
      attacataa aattatgta acgattctt ttctgaagg 
      tgatattcc atcgtttag accaatgtt taacatatc 
      attttaaat acttcattt ttgtacacc tgtccgttt 
      tttattcgc cgatttgga ccaatatcg taagataat 
      ttcgtttct cgaataatt tctctttga gcgtgattt 
      gtgaaatta tgataaaat tgtcaattt tataacgtg 
      acgcaattt tttaaagca agctgatta ttggcaatt 
      ttatatttg tttcttatg aatgatata gatctaaat 
      tattatgta gaaaaacaa aactgtgta atatgaatt 
      ttgctaatt actgtaaat atattgtac attcgttta 
      aggaaatat tatgtctac gcataattg actatttgt 
      ttttctatt tacgttcta gtattttat ggggattat 
      tatttacta cacttattg tgttttatt atattgttc 
      agtacaaat ggaaattta tttttagta ttttctaaa 
      ttagtcctg tatatttta tttcaaatt ttgactttg 
      tgttatcct attttatta tcagtgttt tgtgcaatg 
      aataaaatg tagactgat ag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY