| General information | |||
| TC000880 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 7743071..7751589 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC000880 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00184 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC000880 RA
MLGYTSYRD WSPAGATAQ QQLSVVTTV WGVTTSTQS GPHGGYSGG PTPAGNPNY GNTLKQQQT SYQTAYRQT GPGVGNYGG GNGMNGSEG GYGGGNALS TAAMVAAAT ATATATASV VALQDNSQF STQQYSQGY QQRMMPMNG MSSMNGMNT MNPMSNMAN MGMGNMHNN MMGNMGPKM GMQSPGPTN NSMYPRRMT PYPSPAMHM SQKRGQQYT NSGPCPTMN PNFNPNGQY YGTRQPNFN QYPSQQTLG PSGNFGPAG MGPVVRGAA ALRQTTPPY TNQGQYFPN GPMNQFSND SQYMTRNGQ YGNTNQFQQ DVAMRGNMN YQHSPIPGN PTPPLTPAS SMPPYISPN PDVKPNFNE MKPIIPSQK DDELRLTFP VRDGIILPP FRLEHNLAV SNHVFQLKP TVHQTLMWR SDLELQLKC FHHEDRQMN TNWPASVQV SVNATPLII DRGENKTSH KPLYLKDVC QPGRNTIQI TVSACCCSH LFVLQLVHR PSVRSVLQG LLRKRLLTA DHCIAKIKR NFGNTNSGP NMDRDRDPV EQTALKVSL KCPITYKRI TLPARGHDC KHIQCFDLE SYLQMNCER GAWRCPVCN KPAQLEGLE VDQYMWGIL NTLSNSEVD EVTIDSSAN WKAAKGLGA TIKMEDDND SCGAKRLNK AMSPGSMTL PTMNNWDMS QAMSPYIPP DMNSIASGS MMNGPPSYN NTRNGQYDF GSSTNSGNN EYSAGNGPL SHLSDSVNS LDPLNAMEK SLNEQMPHT PHTPHTPHT PHTPGGTPG SAHTPGGTG PPSVPPATD HHNNSNSSN SSSNNNSGN NEAVPEIPS DLNFDPAAV IDGEGTGQE ALNLLPDSV DPMELLSYL DPPDLNTPP SSGSSAGHA NTSASDDLL ALFE
- >TC000880 RA
atgctggga tatacttcc tacagggac tggtcgcca gcgggcgcc acggcccag cagcagttg tcagtcgtg accacggtg tggggagtc accacgtct actcagtcg ggcccccac ggaggctac tcgggtggc ccgacacct gcaggaaat cccaactac ggaaacacc ctcaaacag cagcagact tcctatcaa acggcctac aggcagacc gggcctggg gttggaaat tatggtggt ggtaatgga atgaacggt agtgaggga ggttacgga ggtggtaat gccctgtca acagctgcg atggtagcg gctgcgaca gcaacggcc accgccacc gcgagcgtg gtggcactc caagacaac agtcaattc tccacccag cagtattct cagggttat cagcaacgc atgatgccc atgaacggg atgagcagc atgaacggc atgaacacc atgaacccc atgtcgaac atggccaat atgggcatg ggcaatatg cacaacaac atgatgggc aacatgggc ccgaaaatg ggcatgcaa tcgccgggg cctaccaac aactccatg tacccgcgc cgaatgacc ccatacccg tcccccgcc atgcacatg tcgcagaaa cgggggcag cagtacacc aactcgggc ccttgcccc accatgaac cccaacttc aaccccaac ggccagtac tatgggacg cggcagccc aacttcaac cagtacccc tcccagcag acgctgggg cccagcggg aacttcggc ccggcgggg atggggccc gtggtgcgg ggggcggcg gcgctgcgg caaaccacc ccgccgtac accaaccaa ggccagtat ttccccaac ggacccatg aaccagttt tcgaatgac tcgcagtac atgacgcgg aatgggcaa tatggcaat accaaccag ttccagcaa gatgtggcc atgaggggc aacatgaac taccagcac agccccatc ccgggcaac cccaccccg ccgctcacc cccgcgagt agcatgcca ccctacatt agccccaat ccggacgtc aagcccaac ttcaacgaa atgaaaccc ataattccc agtcaaaaa gatgacgag ctccggtta actttcccc gtccgagac ggcatcatt ttgcccccc tttcggtta gaacacaat ctcgcggtt agcaatcac gtttttcag ttaaaaccg acggtccat cagacttta atgtggcgt tctgactta gaattacaa ctcaagtgc ttccaccac gaagataga caaatgaac acgaattgg ccggcttcc gtgcaagtc tcagtaaac gcaacacct ctcattata gaccggggc gagaacaag acatcgcat aaacctctg taccttaaa gatgtctgc cagcccgga cgaaacact atccaaatc acagtttcg gcttgttgt tgttcgcac ctcttcgtc ctacaactt gtacataga cccagtgtt agatcagtg cttcaaggc ctcctccgg aaacggctc cttacggcc gatcattgt atagcgaaa attaaacga aacttcggc aataccaac tctggcccg aacatggac cgagaccgg gatcctgtc gaacaaacc gctctcaaa gtctccctc aagtgtcca atcacctac aagaggata acactgcca gcgagagga cacgactgt aaacacatc cagtgcttc gatttggag tcttacctc caaatgaac tgcgagagg ggcgcttgg aggtgtccg gtctgtaat aagccagcg cagctggaa ggcctggaa gtagatcag tacatgtgg ggaattttg aacacgtta agcaacagc gaagtcgac gaagtcacg attgacagc tcggccaac tggaaggcg gccaaaggc ctgggcgcg actatcaag atggaggac gataatgac tcctgtgga gccaaacgg ctgaacaag gcaatgtct ccgggtagt atgacgctg ccgacgatg aacaactgg gacatgtcg caagcaatg tccccctat atacctccc gacatgaac agtattgca agcgggtcg atgatgaac ggccctcct tcctacaac aacactcgg aacggtcag tacgacttc ggatcgtca accaactca ggaaacaat gagtattcg gccggaaat ggccccctg tcccatctg agcgattcg gtcaattcg ctcgatccg ttaaacgcc atggagaag agtttgaac gaacagatg ccacacacg ccccatacg ccgcacacc cctcacacg ccgcacact cccggagga acgcccgga tcggcacac actccggga gggaccggc ccccctagt gttcctcca gcgacggac caccacaac aatagcaac agttcaaat agtagtagt aataataac agtgggaac aacgaggcc gttcccgaa atcccctca gacttgaac tttgaccca gctgcggtt attgacggt gaaggcaca ggccaggaa gcacttaat ttgttgccg gacagcgtg gatccgatg gagttgttg tcgtacttg gaccctcct gacctgaat acgccccca tctagtggt agcagtgca gggcatgcc aacacctcg gcgagtgat gacctcctt gctctgttc gaataa
- >TC000880 RA
cgttgtttc tctcacagg cagacagtt gaacgtcgc gttccggtt gtgtagtac gcgcgcgtt aaaacgctc gctccggta aaactgtgt taacaaaat attatggtc gtgtaaaag tgtctcaaa cattgacta tctttatgt ttaccctca aatgctggg atatacttc ctacaggga ctggtcgcc agcgggcgc cacggccca gcagcagtt gtcagtcgt gaccacggt gtggggagt caccacgtc tactcagtc gggccccca cggaggcta ctcgggtgg cccgacacc tgcaggaaa tcccaacta cggaaacac cctcaaaca gcagcagac ttcctatca aacggccta caggcagac cgggcctgg ggttggaaa ttatggtgg tggtaatgg aatgaacgg tagtgaggg aggttacgg aggtggtaa tgccctgtc aacagctgc gatggtagc ggctgcgac agcaacggc caccgccac cgcgagcgt ggtggcact ccaagacaa cagtcaatt ctccaccca gcagtattc tcagggtta tcagcaacg catgatgcc catgaacgg gatgagcag catgaacgg catgaacac catgaaccc catgtcgaa catggccaa tatgggcat gggcaatat gcacaacaa catgatggg caacatggg cccgaaaat gggcatgca atcgccggg gcctaccaa caactccat gtacccgcg ccgaatgac cccataccc gtcccccgc catgcacat gtcgcagaa acgggggca gcagtacac caactcggg cccttgccc caccatgaa ccccaactt caaccccaa cggccagta ctatgggac gcggcagcc caacttcaa ccagtaccc ctcccagca gacgctggg gcccagcgg gaacttcgg cccggcggg gatggggcc cgtggtgcg gggggcggc ggcgctgcg gcaaaccac cccgccgta caccaacca aggccagta tttccccaa cggacccat gaaccagtt ttcgaatga ctcgcagta catgacgcg gaatgggca atatggcaa taccaacca gttccagca agatgtggc catgagggg caacatgaa ctaccagca cagccccat cccgggcaa ccccacccc gccgctcac ccccgcgag tagcatgcc accctacat tagccccaa tccggacgt caagcccaa cttcaacga aatgaaacc cataattcc cagtcaaaa agatgacga gctccggtt aactttccc cgtccgaga cggcatcat tttgccccc ctttcggtt agaacacaa tctcgcggt tagcaatca cgtttttca gttaaaacc gacggtcca tcagacttt aatgtggcg ttctgactt agaattaca actcaagtg cttccacca cgaagatag acaaatgaa cacgaattg gccggcttc cgtgcaagt ctcagtaaa cgcaacacc tctcattat agaccgggg cgagaacaa gacatcgca taaacctct gtaccttaa agatgtctg ccagcccgg acgaaacac tatccaaat cacagtttc ggcttgttg ttgttcgca cctcttcgt cctacaact tgtacatag acccagtgt tagatcagt gcttcaagg cctcctccg gaaacggct ccttacggc cgatcattg tatagcgaa aattaaacg aaacttcgg caataccaa ctctggccc gaacatgga ccgagaccg ggatcctgt cgaacaaac cgctctcaa agtctccct caagtgtcc aatcaccta caagaggat aacactgcc agcgagagg acacgactg taaacacat ccagtgctt cgatttgga gtcttacct ccaaatgaa ctgcgagag gggcgcttg gaggtgtcc ggtctgtaa taagccagc gcagctgga aggcctgga agtagatca gtacatgtg gggaatttt gaacacgtt aagcaacag cgaagtcga cgaagtcac gattgacag ctcggccaa ctggaaggc ggccaaagg cctgggcgc gactatcaa gatggagga cgataatga ctcctgtgg agccaaacg gctgaacaa ggcaatgtc tccgggtag tatgacgct gccgacgat gaacaactg ggacatgtc gcaagcaat gtcccccta tatacctcc cgacatgaa cagtattgc aagcgggtc gatgatgaa cggccctcc ttcctacaa caacactcg gaacggtca gtacgactt cggatcgtc aaccaactc aggaaacaa tgagtattc ggccggaaa tggccccct gtcccatct gagcgattc ggtcaattc gctcgatcc gttaaacgc catggagaa gagtttgaa cgaacagat gccacacac gccccatac gccgcacac ccctcacac gccgcacac tcccggagg aacgcccgg atcggcaca cactccggg agggaccgg cccccctag tgttcctcc agcgacgga ccaccacaa caatagcaa cagttcaaa tagtagtag taataataa cagtgggaa caacgaggc cgttcccga aatcccctc agacttgaa ctttgaccc agctgcggt tattgacgg tgaaggcac aggccagga agcacttaa tttgttgcc ggacagcgt ggatccgat ggagttgtt gtcgtactt ggaccctcc tgacctgaa tacgccccc atctagtgg tagcagtgc agggcatgc caacacctc ggcgagtga tgacctcct tgctctgtt cgaataaga tgagttcct gagctcacg accacgtct cgtggtgga catgtgagt gaacaaagt gtgataaaa caatctaaa cgtgttaat tattgaagt aaaggtcta aatcaaact attttcact gtattattt gtacataac tacttaaaa gaatggcgt gagttttta tttcattgg ttggataaa gtctattga gatatattt aaattattg agaaggtct attttgatt ttaatgcca aaattttat tgtattata tgagagacc agtggaata aaaagcgac tcgtgacgg ccagtttag tataatatt attaactac atgaatata cttactata gtatagaag ttggaacca aacgatcta gtacttcta gaaacggcc gagttgtcg gtctttttg catctcgat gtgcatagg cttcaagta ttatctgct gtattcgat tcagtgaac ctaatataa cgcagacaa cacgtgtaa cgattccac acaaccaca tgacagtag aaatttcaa attgtattt tgattgtat tatttgttt caggcttaa atatctgca caagcatcc acatataat attattatt aattgagaa ctctgagac tgtgataaa actttaatt ataagtaat tttaacata tttgataat tatatattt ataatcgtg gttctttag cgtcgtttg aatttgtat taaacaaaa atattatgt tatagtgtg gctgctgtt tgtgtgtgt tttcactaa agttaattt gatgtgata aaatatatt ttggttaaa tacaatttg agttttatt cccaaatcc ttatacgtg tttgtcact tgtattgat ttaagtagg gtccaagtt cctaaaagc aaaaatgta ttcactttg tattgatac aaattatat caggttcac tcgaattgc gcaattctt attgccaag aataactca ttctaaaag gtaggcaga atagatgtc aataagttg tatgtaact agccaaagt agagaattt ttatcaaat tattgaaag tttacaggc acatgacaa catacaaaa gtttatcca atcacgcgg tgtaaactt ttaataagt tcagtataa attttgcca ataatttat tgtttttca ttagcttca ttttcagaa aagggactt ttgtaaatt attttcgac aatggacca attacataa aattatgta acgattctt ttctgaagg tgatattcc atcgtttag accaatgtt taacatatc attttaaat acttcattt ttgtacacc tgtccgttt tttattcgc cgatttgga ccaatatcg taagataat ttcgtttct cgaataatt tctctttga gcgtgattt gtgaaatta tgataaaat tgtcaattt tataacgtg acgcaattt tttaaagca agctgatta ttggcaatt ttatatttg tttcttatg aatgatata gatctaaat tattatgta gaaaaacaa aactgtgta atatgaatt ttgctaatt actgtaaat atattgtac attcgttta aggaaatat tatgtctac gcataattg actatttgt ttttctatt tacgttcta gtattttat ggggattat tatttacta cacttattg tgttttatt atattgttc agtacaaat ggaaattta tttttagta ttttctaaa ttagtcctg tatatttta tttcaaatt ttgactttg tgttatcct attttatta tcagtgttt tgtgcaatg aataaaatg tagactgat ag
