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Gene information
General information
TC000876 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 2110688..2112449
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000876
    iBeetle screen iB_00183
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000876 RA
      MGSNNVNSV IVVGGGLVG SLCAIFMAK RGYHVTLFE 
      YREDIRTAK FARGRSINM ALSNRGRKA LRAVGIEKI 
      ILESAIPMK GRLLHDLKG RTTSVPYDA LTGQCIYSI 
      SRNYLNNVL LTELEKYPN VKIYFNHKL MSVSFEDER 
      ISVMNLITE EITTHQADL IIGADGAYS TLRRYMQLT 
      PLFEYSQTY IQHGYLELV IPPENGPKM TPNHLHIWP 
      RGQFMMIAL PNKDNSWTV TLFMPFAKF ESLRNAAEL 
      KDFYYKTFP DAVPLIGED LLVNDFFKV KPSALVSVK 
      CKPYHVGSK FLLIGDAAH AMVPFYGQG MNAGFEDCF 
      LLDGILERR SNDIAGSIE EFSRERVED AYAICELAM 
      YNYVEMRDL VTRPSYRLR KFFDELLFK CMKEKWIPL 
      YNSVTFSNF GYKQCVENR KWQNKVIQK FLWTGGLVT 
      SALFAYGLR IWLY
    CDS sequence
    • >TC000876 RA
      atgggtagt aataacgtc aattcggtt attgtagtg 
      ggcggtggt ttggttggt tctttgtgt gctattttt 
      atggcaaag aggggctac catgttaca ctttttgag 
      tatcgggaa gatattagg acggcaaag tttgctcgt 
      ggccgttcg ataaatatg gctttgtcg aaccgtggc 
      agaaaagcg ctacgtgct gttggaata gaaaaaata 
      attcttgaa tcggcgatt cctatgaaa gggcggctt 
      ttacacgac ttgaagggg cgaaccact agtgtacca 
      tacgatgct ttaacggga caatgtatt tattcgatc 
      agcagaaat tacttaaat aacgtgctg ttaaccgaa 
      ttagagaag tacccaaat gtgaaaatt tatttcaat 
      cataaatta atgagtgtg agctttgag gatgagaga 
      atttcggtc atgaatttg ataactgaa gaaattacg 
      acccaccaa gccgattta ataataggg gccgatggg 
      gcctacagt acccttcgg cgttacatg cagctgacc 
      cctcttttt gagtacagc caaacctac atccaacac 
      gggtacctc gagttggtc atccctccc gaaaacggg 
      cccaaaatg acccccaat catctacac atctggccc 
      cggggccaa ttcatgatg attgcgtta cccaacaag 
      gacaattcc tggactgtc accctgttt atgcctttt 
      gctaaattc gaaagtttg agaaatgct gcagaattg 
      aaagatttt tactacaag actttcccc gacgctgtc 
      cccctcatc ggtgaggat ctcctcgtg aacgatttt 
      tttaaagtg aagccatcg gctctggtc tcggtcaag 
      tgtaaaccg taccatgtg gggagcaaa tttctgctg 
      attggggac gcggcacat gccatggta cccttttat 
      gggcagggg atgaatgca gggtttgag gattgtttc 
      cttttggac ggcattcta gaaaggagg agtaacgac 
      atcgcgggg tctattgag gagttcagt agggaacgg 
      gttgaggat gcttatgct atttgcgag ttggcgatg 
      tataattac gtggaaatg agggatctt gtgactagg 
      ccttcgtac cgtttgaga aaatttttc gatgaattg 
      ctttttaag tgtatgaag gagaagtgg attccgttg 
      tacaattcg gtgactttt tcgaatttc gggtacaag 
      cagtgtgtg gaaaacagg aagtggcaa aacaaggtt 
      attcaaaaa tttctctgg acgggtggc ttggtaact 
      agcgctttg tttgcatat ggattacgg atttggttg 
      tattaa
    mRNA sequence
    • >TC000876 RA
      ttgagaaag aaaaaggcg caagccccg aaatgtaac 
      acaacacta acacgatta ttctacata tcaagtggc 
      tgaaacgaa gcctttaaa ctagaacca gtcctgtgt 
      tggtggttt ttgtgtgaa aatgggtag taataacgt 
      caattcggt tattgtagt gggcggtgg tttggttgg 
      ttctttgtg tgctatttt tatggcaaa gaggggcta 
      ccatgttac actttttga gtatcggga agatattag 
      gacggcaaa gtttgctcg tggccgttc gataaatat 
      ggctttgtc gaaccgtgg cagaaaagc gctacgtgc 
      tgttggaat agaaaaaat aattcttga atcggcgat 
      tcctatgaa agggcggct tttacacga cttgaaggg 
      gcgaaccac tagtgtacc atacgatgc tttaacggg 
      acaatgtat ttattcgat cagcagaaa ttacttaaa 
      taacgtgct gttaaccga attagagaa gtacccaaa 
      tgtgaaaat ttatttcaa tcataaatt aatgagtgt 
      gagctttga ggatgagag aatttcggt catgaattt 
      gataactga agaaattac gacccacca agccgattt 
      aataatagg ggccgatgg ggcctacag tacccttcg 
      gcgttacat gcagctgac ccctctttt tgagtacag 
      ccaaaccta catccaaca cgggtacct cgagttggt 
      catccctcc cgaaaacgg gcccaaaat gacccccaa 
      tcatctaca catctggcc ccggggcca attcatgat 
      gattgcgtt acccaacaa ggacaattc ctggactgt 
      caccctgtt tatgccttt tgctaaatt cgaaagttt 
      gagaaatgc tgcagaatt gaaagattt ttactacaa 
      gactttccc cgacgctgt ccccctcat cggtgagga 
      tctcctcgt gaacgattt ttttaaagt gaagccatc 
      ggctctggt ctcggtcaa gtgtaaacc gtaccatgt 
      ggggagcaa atttctgct gattgggga cgcggcaca 
      tgccatggt accctttta tgggcaggg gatgaatgc 
      agggtttga ggattgttt ccttttgga cggcattct 
      agaaaggag gagtaacga catcgcggg gtctattga 
      ggagttcag tagggaacg ggttgagga tgcttatgc 
      tatttgcga gttggcgat gtataatta cgtggaaat 
      gagggatct tgtgactag gccttcgta ccgtttgag 
      aaaattttt cgatgaatt gctttttaa gtgtatgaa 
      ggagaagtg gattccgtt gtacaattc ggtgacttt 
      ttcgaattt cgggtacaa gcagtgtgt ggaaaacag 
      gaagtggca aaacaaggt tattcaaaa atttctctg 
      gacgggtgg cttggtaac tagcgcttt gtttgcata 
      tggattacg gatttggtt gtattaaaa tatttgcgt 
      tttttatca gtttaattt attgtaaga cttttctga 
      ataaaacaa atatttaaa c