| General information | |||
| TC000861 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 7195494..7222041 | |
| Sequence information | |||
| Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC000861 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00182 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC000861 RA
MPSALLVLV VLAFSPLVH GRHARNTES NGISVLGEF NHEAFPRFA EPIPNVTVT VGRDALLAC VVDNLRAFK VAWVRVDTQ TILSIHHNV ITQNPRISL SYNDHRSWF LHIRNVQES DRGWYMCQV NTDPMRSRQ GYLQVVVPP SIVDRETSS DMVVLESTN VSLTCKATG YPEPYVMWR REDGEDIRY NGENVNVVD GEVLFITKV SRLHMAVYL CIASNGVPP SISKRVQLK VQFPPMLSI PNQLEGAYI GQDVTLECR TEAFPTSIN YWTTERGDM IISGSDKYE AVSMDNGYK KYMMLKIRR VNKSDFGSY KCVAKNSLG ETDGVIKLE EIPAPTTLK TTTQFPSLP TKKKGKTRA GDRFGRGEA NNRVDSPPD FDDAGMPEY DDDGEFTLS SNGSPPIQE PPGAFSGAT RTENFNWCI GGVLAMTWI FSKGLR
- >TC000861 RB
MPSALLVLV VLAFSPLVH GRHARNTES NGISVLGEF NHEAFPRFA EPIPNVTVT VGRDALLAC VVDNLRAFK VAWVRVDTQ TILSIHHNV ITQNPRISL SYNDHRSWF LHIRNVQES DRGWYMCQV NTDPMRSRQ GYLQVVVPP SIVDRETSS DMVVLESTN VSLTCKATG YPEPYVMWR REDGEDIRY NGENVNVVD GEVLFITKV SRLHMAVYL CIASNGVPP SISKRVQLK VQFPPMLSI PNQLEGAYI GQDVTLECR TEAFPTSIN YWTTERGDM IISGDKYEA VSMDNGYKK YMMLKIRRV NKSDFGSYK CVAKNSLGE TDGVIKLEE IPAPTTLKT TTQFPSLPT KKKGKTRAG DRFGRGEAN NRVDSPPDF DDAGMPEYD DDGEFTLSS NGSPPIQEP PGAFSGATR TENFNWCIG GVLAMTWIF SKGLR
- >TC000861 RC
MPSALLVLV VLAFSPLVH GRHARNTES NGISVLGEF NHEAFPRFA EPIPNVTVT VGRDALLAC VVDNLRAFK VAWVRVDTQ TILSIHHNV ITQNPRISL SYNDHRSWF LHIRNVQES DRGWYMCQV NTDPMRSRQ GYLQVVVPP SIVDRETSS DMVVLESTN VSLTCKATG YPEPYVMWR REDGEDIRY NGENVNVVD GEVLFITKV SRLHMAVYL CIASNGVPP SISKRVQLK VQFPPMLSI PNQLEGAYI GQDVTLECR TEAFPTSIN YWTTERGDM IISGSDKYE AVSMDNGYK KYMMLKIRR VNKSDFGSY KCVAKNSLG ETDGVIKLE EIPAPTTLK TTTQFPSLP TKKKEYDDD GEFTLSSNG SPPIQEPPG AFSGATRTE NFNWCIGGV LAMTWIFSK GLR
- >TC000861 RA
atgccttcc gcgctactc gtgttggtg gtgctggcc ttctctcca ctagtgcac gggaggcac gcgaggaac acagagtcg aacggcatc tcagtccta ggagaattt aatcacgag gccttcccg cgcttcgcc gagcccatc cctaacgta acggtgact gtgggccgg gacgccctt ctggcttgc gtggtcgac aacctgcga gcattcaag gtagcttgg gtcagagtg gacactcag acgattcta tctatccac cacaatgtc atcacgcaa aacccgagg atatcgtta agctacaac gatcatcga tcgtggttt ttgcatatc cggaacgtg caagaatct gatcggggt tggtacatg tgtcaagtc aacaccgac ccaatgcgg agccgccaa ggctatctc caagtggtc gtgcctccc agtatcgtg gaccgcgaa accagctcc gacatggtg gtgctggag tcgacaaac gtcagtctg acctgcaaa gcgacgggt tacccggaa ccgtatgtc atgtggcgg agagaagac ggagaagac atccgatac aatggagag aacgtgaac gttgtcgat ggagaggta ttatttatt actaaagtt agccggctt cacatggcc gtgtatttg tgcatcgcc tcaaacggc gttccccct tccataagc aagagggtt cagttgaaa gtacaattt cctccgatg ctatcgatt ccgaatcaa ttggaggga gcttacata gggcaagat gtcactctc gagtgccgc actgaggca tttccgacg tccatcaac tactggacg acggaaaga ggagacatg atcatctca ggtagtgat aagtacgaa gcagtatct atggataat ggctataaa aaatacatg atgttgaaa attcgacga gtcaacaaa tcggatttc ggctcctac aagtgcgtc gccaagaat tccctcggg gaaactgac ggcgtgata aaactggaa gagattcca gcaccgacg acactaaaa acaacaacc cagtttccg tcattacct acgaaaaag aaaggtaaa actcgggct ggagataga ttcggacgc ggagaagct aacaaccga gtcgattca cctcccgac tttgacgat gccggaatg ccagaatac gacgatgac ggagagttc actttgtcg tcgaacggc tctccgccg attcaggag cctccggga gctttcagc ggtgcaacg cgcactgaa aactttaac tggtgtatc ggtggcgtt ctcgcgatg acatggata ttcagtaaa ggcctgagg tga
- >TC000861 RB
atgccttcc gcgctactc gtgttggtg gtgctggcc ttctctcca ctagtgcac gggaggcac gcgaggaac acagagtcg aacggcatc tcagtccta ggagaattt aatcacgag gccttcccg cgcttcgcc gagcccatc cctaacgta acggtgact gtgggccgg gacgccctt ctggcttgc gtggtcgac aacctgcga gcattcaag gtagcttgg gtcagagtg gacactcag acgattcta tctatccac cacaatgtc atcacgcaa aacccgagg atatcgtta agctacaac gatcatcga tcgtggttt ttgcatatc cggaacgtg caagaatct gatcggggt tggtacatg tgtcaagtc aacaccgac ccaatgcgg agccgccaa ggctatctc caagtggtc gtgcctccc agtatcgtg gaccgcgaa accagctcc gacatggtg gtgctggag tcgacaaac gtcagtctg acctgcaaa gcgacgggt tacccggaa ccgtatgtc atgtggcgg agagaagac ggagaagac atccgatac aatggagag aacgtgaac gttgtcgat ggagaggta ttatttatt actaaagtt agccggctt cacatggcc gtgtatttg tgcatcgcc tcaaacggc gttccccct tccataagc aagagggtt cagttgaaa gtacaattt cctccgatg ctatcgatt ccgaatcaa ttggaggga gcttacata gggcaagat gtcactctc gagtgccgc actgaggca tttccgacg tccatcaac tactggacg acggaaaga ggagacatg atcatctca ggtgataag tacgaagca gtatctatg gataatggc tataaaaaa tacatgatg ttgaaaatt cgacgagtc aacaaatcg gatttcggc tcctacaag tgcgtcgcc aagaattcc ctcggggaa actgacggc gtgataaaa ctggaagag attccagca ccgacgaca ctaaaaaca acaacccag tttccgtca ttacctacg aaaaagaaa ggtaaaact cgggctgga gatagattc ggacgcgga gaagctaac aaccgagtc gattcacct cccgacttt gacgatgcc ggaatgcca gaatacgac gatgacgga gagttcact ttgtcgtcg aacggctct ccgccgatt caggagcct ccgggagct ttcagcggt gcaacgcgc actgaaaac tttaactgg tgtatcggt ggcgttctc gcgatgaca tggatattc agtaaaggc ctgaggtga
- >TC000861 RC
atgccttcc gcgctactc gtgttggtg gtgctggcc ttctctcca ctagtgcac gggaggcac gcgaggaac acagagtcg aacggcatc tcagtccta ggagaattt aatcacgag gccttcccg cgcttcgcc gagcccatc cctaacgta acggtgact gtgggccgg gacgccctt ctggcttgc gtggtcgac aacctgcga gcattcaag gtagcttgg gtcagagtg gacactcag acgattcta tctatccac cacaatgtc atcacgcaa aacccgagg atatcgtta agctacaac gatcatcga tcgtggttt ttgcatatc cggaacgtg caagaatct gatcggggt tggtacatg tgtcaagtc aacaccgac ccaatgcgg agccgccaa ggctatctc caagtggtc gtgcctccc agtatcgtg gaccgcgaa accagctcc gacatggtg gtgctggag tcgacaaac gtcagtctg acctgcaaa gcgacgggt tacccggaa ccgtatgtc atgtggcgg agagaagac ggagaagac atccgatac aatggagag aacgtgaac gttgtcgat ggagaggta ttatttatt actaaagtt agccggctt cacatggcc gtgtatttg tgcatcgcc tcaaacggc gttccccct tccataagc aagagggtt cagttgaaa gtacaattt cctccgatg ctatcgatt ccgaatcaa ttggaggga gcttacata gggcaagat gtcactctc gagtgccgc actgaggca tttccgacg tccatcaac tactggacg acggaaaga ggagacatg atcatctca ggtagtgat aagtacgaa gcagtatct atggataat ggctataaa aaatacatg atgttgaaa attcgacga gtcaacaaa tcggatttc ggctcctac aagtgcgtc gccaagaat tccctcggg gaaactgac ggcgtgata aaactggaa gagattcca gcaccgacg acactaaaa acaacaacc cagtttccg tcattacct acgaaaaag aaagaatac gacgatgac ggagagttc actttgtcg tcgaacggc tctccgccg attcaggag cctccggga gctttcagc ggtgcaacg cgcactgaa aactttaac tggtgtatc ggtggcgtt ctcgcgatg acatggata ttcagtaaa ggcctgagg tga
- >TC000861 RA
gaccagcaa ccggactcg aatcagatt ccgcgtgcc ctccggagc gacagctag tgcataggt gatgctctc ggtcagtcg ttgtcatgc cttccgcgc tactcgtgt tggtggtgc tggccttct ctccactag tgcacggga ggcacgcga ggaacacag agtcgaacg gcatctcag tcctaggag aatttaatc acgaggcct tcccgcgct tcgccgagc ccatcccta acgtaacgg tgactgtgg gccgggacg cccttctgg cttgcgtgg tcgacaacc tgcgagcat tcaaggtag cttgggtca gagtggaca ctcagacga ttctatcta tccaccaca atgtcatca cgcaaaacc cgaggatat cgttaagct acaacgatc atcgatcgt ggtttttgc atatccgga acgtgcaag aatctgatc ggggttggt acatgtgtc aagtcaaca ccgacccaa tgcggagcc gccaaggct atctccaag tggtcgtgc ctcccagta tcgtggacc gcgaaacca gctccgaca tggtggtgc tggagtcga caaacgtca gtctgacct gcaaagcga cgggttacc cggaaccgt atgtcatgt ggcggagag aagacggag aagacatcc gatacaatg gagagaacg tgaacgttg tcgatggag aggtattat ttattacta aagttagcc ggcttcaca tggccgtgt atttgtgca tcgcctcaa acggcgttc ccccttcca taagcaaga gggttcagt tgaaagtac aatttcctc cgatgctat cgattccga atcaattgg agggagctt acatagggc aagatgtca ctctcgagt gccgcactg aggcatttc cgacgtcca tcaactact ggacgacgg aaagaggag acatgatca tctcaggta gtgataagt acgaagcag tatctatgg ataatggct ataaaaaat acatgatgt tgaaaattc gacgagtca acaaatcgg atttcggct cctacaagt gcgtcgcca agaattccc tcggggaaa ctgacggcg tgataaaac tggaagaga ttccagcac cgacgacac taaaaacaa caacccagt ttccgtcat tacctacga aaaagaaag gtaaaactc gggctggag atagattcg gacgcggag aagctaaca accgagtcg attcacctc ccgactttg acgatgccg gaatgccag aatacgacg atgacggag agttcactt tgtcgtcga acggctctc cgccgattc aggagcctc cgggagctt tcagcggtg caacgcgca ctgaaaact ttaactggt gtatcggtg gcgttctcg cgatgacat ggatattca gtaaaggcc tgaggtgat ccgacatat ccaattaaa aactacacg gagaagatt cgtggtgat aaaaacata tccagtgcg agagactga gctgtaaat atgtacaaa cagtgctga acttagata ataacaaag ttgtgtata cagttgctg cgctaaagt gtatattag aaactgcct acatttgaa tagtgcgtg atattacaa tacatgtat gtagcatag tgtcaaatg aatcaataa agtttaaat aacatt
- >TC000861 RB
gaccagcaa ccggactcg aatcagatt ccgcgtgcc ctccggagc gacagctag tgcataggt gatgctctc ggtcagtcg ttgtcatgc cttccgcgc tactcgtgt tggtggtgc tggccttct ctccactag tgcacggga ggcacgcga ggaacacag agtcgaacg gcatctcag tcctaggag aatttaatc acgaggcct tcccgcgct tcgccgagc ccatcccta acgtaacgg tgactgtgg gccgggacg cccttctgg cttgcgtgg tcgacaacc tgcgagcat tcaaggtag cttgggtca gagtggaca ctcagacga ttctatcta tccaccaca atgtcatca cgcaaaacc cgaggatat cgttaagct acaacgatc atcgatcgt ggtttttgc atatccgga acgtgcaag aatctgatc ggggttggt acatgtgtc aagtcaaca ccgacccaa tgcggagcc gccaaggct atctccaag tggtcgtgc ctcccagta tcgtggacc gcgaaacca gctccgaca tggtggtgc tggagtcga caaacgtca gtctgacct gcaaagcga cgggttacc cggaaccgt atgtcatgt ggcggagag aagacggag aagacatcc gatacaatg gagagaacg tgaacgttg tcgatggag aggtattat ttattacta aagttagcc ggcttcaca tggccgtgt atttgtgca tcgcctcaa acggcgttc ccccttcca taagcaaga gggttcagt tgaaagtac aatttcctc cgatgctat cgattccga atcaattgg agggagctt acatagggc aagatgtca ctctcgagt gccgcactg aggcatttc cgacgtcca tcaactact ggacgacgg aaagaggag acatgatca tctcaggtg ataagtacg aagcagtat ctatggata atggctata aaaaataca tgatgttga aaattcgac gagtcaaca aatcggatt tcggctcct acaagtgcg tcgccaaga attccctcg gggaaactg acggcgtga taaaactgg aagagattc cagcaccga cgacactaa aaacaacaa cccagtttc cgtcattac ctacgaaaa agaaaggta aaactcggg ctggagata gattcggac gcggagaag ctaacaacc gagtcgatt cacctcccg actttgacg atgccggaa tgccagaat acgacgatg acggagagt tcactttgt cgtcgaacg gctctccgc cgattcagg agcctccgg gagctttca gcggtgcaa cgcgcactg aaaacttta actggtgta tcggtggcg ttctcgcga tgacatgga tattcagta aaggcctga ggtgatccg acatatcca attaaaaac tacacggag aagattcgt ggtgataaa aacatatcc agtgcgaga gactgagct gtaaatatg tacaaacag tgctgaact tagataata acaaagttg tgtatacag ttgctgcgc taaagtgta tattagaaa ctgcctaca tttgaatag tgcgtgata ttacaatac atgtatgta gcatagtgt caaatgaat caataaagt ttaaataac att
- >TC000861 RC
gaccagcaa ccggactcg aatcagatt ccgcgtgcc ctccggagc gacagctag tgcataggt gatgctctc ggtcagtcg ttgtcatgc cttccgcgc tactcgtgt tggtggtgc tggccttct ctccactag tgcacggga ggcacgcga ggaacacag agtcgaacg gcatctcag tcctaggag aatttaatc acgaggcct tcccgcgct tcgccgagc ccatcccta acgtaacgg tgactgtgg gccgggacg cccttctgg cttgcgtgg tcgacaacc tgcgagcat tcaaggtag cttgggtca gagtggaca ctcagacga ttctatcta tccaccaca atgtcatca cgcaaaacc cgaggatat cgttaagct acaacgatc atcgatcgt ggtttttgc atatccgga acgtgcaag aatctgatc ggggttggt acatgtgtc aagtcaaca ccgacccaa tgcggagcc gccaaggct atctccaag tggtcgtgc ctcccagta tcgtggacc gcgaaacca gctccgaca tggtggtgc tggagtcga caaacgtca gtctgacct gcaaagcga cgggttacc cggaaccgt atgtcatgt ggcggagag aagacggag aagacatcc gatacaatg gagagaacg tgaacgttg tcgatggag aggtattat ttattacta aagttagcc ggcttcaca tggccgtgt atttgtgca tcgcctcaa acggcgttc ccccttcca taagcaaga gggttcagt tgaaagtac aatttcctc cgatgctat cgattccga atcaattgg agggagctt acatagggc aagatgtca ctctcgagt gccgcactg aggcatttc cgacgtcca tcaactact ggacgacgg aaagaggag acatgatca tctcaggta gtgataagt acgaagcag tatctatgg ataatggct ataaaaaat acatgatgt tgaaaattc gacgagtca acaaatcgg atttcggct cctacaagt gcgtcgcca agaattccc tcggggaaa ctgacggcg tgataaaac tggaagaga ttccagcac cgacgacac taaaaacaa caacccagt ttccgtcat tacctacga aaaagaaag aatacgacg atgacggag agttcactt tgtcgtcga acggctctc cgccgattc aggagcctc cgggagctt tcagcggtg caacgcgca ctgaaaact ttaactggt gtatcggtg gcgttctcg cgatgacat ggatattca gtaaaggcc tgaggtgat ccgacatat ccaattaaa aactacacg gagaagatt cgtggtgat aaaaacata tccagtgcg agagactga gctgtaaat atgtacaaa cagtgctga acttagata ataacaaag ttgtgtata cagttgctg cgctaaagt gtatattag aaactgcct acatttgaa tagtgcgtg atattacaa tacatgtat gtagcatag tgtcaaatg aatcaataa agtttaaat aacatt
