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Gene information
General information
TC000855 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 1446055..1456396
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000855
    iBeetle screen iB_00181
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition >5 number 2nd lateral oviduct: number 1st lateral oviduct: blocked - Number of animals showing the phenotype: 1

    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 3

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000855 RA
      MTKNKQHKF PLMRATFAT LSRRFRNKD KLKREVRTV 
      ILQKGGSGL GFNIVGGED GEGIFISFI LAGGPADLS 
      GELRRGDQI LSVNGVNLR NATHEEAAQ TLKGTSSTV 
      TMVVQYRPE EYNRFEAKI HDLKQQISH PVTGTLLRT 
      SQKRSLYVR ALFDYDPMK DDGLPSRGL AFHYGDILH 
      VTNASDDEW WQARRVLNT GDEQGMGIV PSKRRWERK 
      QRARDRSVK FQGHMPNIM DKQSTLDRK KKNFSFSRK 
      FPFMKSKDD KSEDGSDQE HSPSATPNS ANDNERNPS 
      SFMLCYTQE DANAEGNEE NVLSYEPVQ QMAINYTRP 
      VIILGPLKD RINDDLISE FPDKFGSCV PHTTRPKRE 
      YEVDARDYH FVSSREQME KDIQNHLFI EAGQYNDNL 
      YGTSVASVR EVADKGKHC ILDVSGNAI KRLQVAQLF 
      PIAIFIKPK SVESIMEMN KRMTEEQAK KTFERSLKM 
      EQEFGEYFT AVVQGDTPE DIYEQVKEV IKEQSGPTI 
      WVPTKEKL
    CDS sequence
    • >TC000855 RA
      atgacgaag aacaagcag cacaagttc ccgctgatg 
      cgagcaacg ttcgcaacc ctcagcaga agattcagg 
      aataaagat aagctgaaa agagaagtg cgcactgtg 
      atactacaa aaaggaggc tccggtttg ggttttaac 
      attgtggga ggtgaagac ggagaagga atcttcata 
      tcattcatt ctggctgga ggtccggcc gatttgagt 
      ggtgaattg cgtcgaggc gatcaaata ctcagcgtg 
      aacggcgtc aacctacga aatgccact cacgaagaa 
      gccgctcaa actctcaaa ggaacgagt tcgacggtc 
      acaatggtg gtgcagtac agacccgag gagtacaac 
      cgcttcgag gccaaaatc catgacttg aaacagcaa 
      atctcgcat cccgtcacg ggaactcta cttaggaca 
      tcacagaaa cgctctctc tacgtcaga gctctgttc 
      gattatgat ccgatgaaa gacgacggg ttgccgagc 
      cggggactc gcttttcat tatggggat atcctacat 
      gtgacgaat gccagtgat gatgagtgg tggcaggcg 
      cggagggta cttaacacg ggggatgaa cagggtatg 
      gggattgtt ccgtcgaag aggaggtgg gagaggaag 
      caaagggcg agggatcgc tcggttaag ttccaagga 
      cacatgccc aacattatg gataagcaa tcgacatta 
      gataggaaa aagaaaaac ttctcgttt agtagaaag 
      ttccccttc atgaaaagt aaagacgac aagtcggaa 
      gacggctca gatcaagaa cactctcct tctgccact 
      cctaactct gctaatgat aatgaacga aatccctca 
      tcgttcatg ttatgctac acccaggag gacgccaat 
      gctgaaggc aatgaggaa aatgtactc tcatacgaa 
      cctgtccag cagatggca atcaattac acgaggccg 
      gttattatt ttggggcct ctcaaagac cgcattaac 
      gacgacctc atttccgaa ttccccgac aagttcgga 
      agttgtgtc ccacatacg actaggccc aagcgggag 
      tacgaagtt gacgcaagg gattatcat tttgtcagc 
      tcgagggaa cagatggag aaggacata caaaatcac 
      ctgttcata gaagcggga caatataat gataatttg 
      tacggaacc tcggtcgcc tcagttagg gaggtggca 
      gataagggc aaacactgc attttggac gtaagtggc 
      aatgcaatc aagaggcta caagtcgct caactcttc 
      cctattgct attttcatc aaacccaaa tcggtcgaa 
      tcaattatg gaaatgaat aagagaatg acggaggaa 
      caagcaaag aaaacattc gaaagatct ctcaagatg 
      gagcaagag tttggggaa tatttcaca gcggtggtg 
      caaggagat acgcctgaa gacatctac gagcaagtg 
      aaagaagta atcaaggag cagtcgggt ccaacaatt 
      tgggtgcca acgaaggaa aaactgtga
    mRNA sequence
    • >TC000855 RA
      tatgagttg ttacagaag ctatctaat aattcagtg 
      gcaaaatgg tgacacgtt tcgcgtctt tgtaaaaag 
      tcggtttga aataagtgt tggaaaatg acgaagaac 
      aagcagcac aagttcccg ctgatgcga gcaacgttc 
      gcaaccctc agcagaaga ttcaggaat aaagataag 
      ctgaaaaga gaagtgcgc actgtgata ctacaaaaa 
      ggaggctcc ggtttgggt tttaacatt gtgggaggt 
      gaagacgga gaaggaatc ttcatatca ttcattctg 
      gctggaggt ccggccgat ttgagtggt gaattgcgt 
      cgaggcgat caaatactc agcgtgaac ggcgtcaac 
      ctacgaaat gccactcac gaagaagcc gctcaaact 
      ctcaaagga acgagttcg acggtcaca atggtggtg 
      cagtacaga cccgaggag tacaaccgc ttcgaggcc 
      aaaatccat gacttgaaa cagcaaatc tcgcatccc 
      gtcacggga actctactt aggacatca cagaaacgc 
      tctctctac gtcagagct ctgttcgat tatgatccg 
      atgaaagac gacgggttg ccgagccgg ggactcgct 
      tttcattat ggggatatc ctacatgtg acgaatgcc 
      agtgatgat gagtggtgg caggcgcgg agggtactt 
      aacacgggg gatgaacag ggtatgggg attgttccg 
      tcgaagagg aggtgggag aggaagcaa agggcgagg 
      gatcgctcg gttaagttc caaggacac atgcccaac 
      attatggat aagcaatcg acattagat aggaaaaag 
      aaaaacttc tcgtttagt agaaagttc cccttcatg 
      aaaagtaaa gacgacaag tcggaagac ggctcagat 
      caagaacac tctccttct gccactcct aactctgct 
      aatgataat gaacgaaat ccctcatcg ttcatgtta 
      tgctacacc caggaggac gccaatgct gaaggcaat 
      gaggaaaat gtactctca tacgaacct gtccagcag 
      atggcaatc aattacacg aggccggtt attattttg 
      gggcctctc aaagaccgc attaacgac gacctcatt 
      tccgaattc cccgacaag ttcggaagt tgtgtccca 
      catacgact aggcccaag cgggagtac gaagttgac 
      gcaagggat tatcatttt gtcagctcg agggaacag 
      atggagaag gacatacaa aatcacctg ttcatagaa 
      gcgggacaa tataatgat aatttgtac ggaacctcg 
      gtcgcctca gttagggag gtggcagat aagggcaaa 
      cactgcatt ttggacgta agtggcaat gcaatcaag 
      aggctacaa gtcgctcaa ctcttccct attgctatt 
      ttcatcaaa cccaaatcg gtcgaatca attatggaa 
      atgaataag agaatgacg gaggaacaa gcaaagaaa 
      acattcgaa agatctctc aagatggag caagagttt 
      ggggaatat ttcacagcg gtggtgcaa ggagatacg 
      cctgaagac atctacgag caagtgaaa gaagtaatc 
      aaggagcag tcgggtcca acaatttgg gtgccaacg 
      aaggaaaaa ctgtgacaa acggcccct ccagtgtgg 
      acgctgcca tcgcgctca cggctgcac tgaacagtt 
      ttgcgaaaa atagacaca ttctgttgt tatgtgcgg 
      tgcctccta atgcgtgct tacaagacc agctcattc 
      attaatcat acctttatt attgcccaa attgttgat 
      aatgacaaa tacaagagt agttatgaa tttaaagta 
      atcttagtg tatggtgcg gcggtgaga gaaacaccc 
      gcaaagctt gtatagatg tggataacc tccgagccc 
      ttatttcat tattaaaaa tgcacttgt gttacttaa 
      tgaattaat ctatgatga ataagcata atagtcaaa 
      tcttgaaat cgtttataa tataattag ccaattgtt 
      atttgactt ttaggtagt tttatcaat gttttttgt 
      agtgtacga tgtatattt aatataaag gtttccctg 
      ccaataagt taaattgta tatacacaa atgatatat 
      gtgtagtat ataacagaa atctgttat tgttatttg 
      agacaagtt gtgacctaa cttatttga gttaataaa 
      agaaatttt actaa