Status
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Gene information
General information
TC000847 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6735745..6746137
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000847
    iBeetle screen iB_00180

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature differentiation incomplete - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000847 RA
      MAYSPIEQD CDLSDYDLY SKTPPRTKI LRKNRERKQ 
      KHKDTSTMS SPKVIKPKP LSRRNTISS MFEELVAKA 
      ENDSDQGSK RSNKRQLKM LRGSERDLK LDIASAQAA 
      SEKNQEFKH PKTPQPSYQ IESCKRFMS LSCRTVPCS 
      KLLKSKKDN KVKCDEISN EECSENRVD FYNTFSMLI 
      RMGCGDKTQ EMSHRRALS KEEHLWQNE FKDLIWLEL 
      QAFHADRTP SEEDRYLCS EREKVEPLL HDIMTYRYQ 
      KNSKCYSTQ SSDSGVEDD CSGCLSIYC GSCLEAQNE 
      ALKDVEELM QRLSAVEAL FPSGHAFAE LYPLYNSPE 
      FIARVKAIS LWYNMTRQQ RLKLMILVK LLENKVSRD 
      DDKNGTESN SPSDSNNSS SSSVNESFY DCKSSQDAT 
      VFSFAPFPF LHVHKSESS VSPYRKYIE NILKTRSLH 
      KSLSFLDHL QMVVLRRVQ LTLTKPETD DIYNKVTCE 
      TEDEELQRF GYFSPEAKA LNLPSYRAI FLFLAAIPL 
      KMIHEFLKM RLEQKPEKP SPLSVRQLM RELKEGLKV 
      ATQQKEKIL KYIDGAISD SKKPNDVFE EKMRDYNEC 
      MLRVFSDYL DYLEQWALL HHETFQKNL LEEEWHFSC 
      NVIKYIPGG SELLGKKFV FILCSILKS VAERLHDRI 
      EDVLCNMPK DDKNMIKQT VFVICREMQ SLFNEEREM 
      SIKTIAFCK TVFRHDLHP DCRSELVCS IISLKCSIP 
      DAIGRVQSE FDQVKLDSF EEIERTAIV SRTREILMQ 
      AYRFGFEFY KEMSDSIPT DRREKLARS MVQFANLWM 
      KFVTERCER GRGMRPRWA YQGLEFLLT VCEPRNTKH 
      LTEEEFEDL KKNMDVCIS HVIGTTAPS TPESGFYSA 
      SPRSSIEHI RARSRGSSP SPRPSYKSQ RSNSRKTSM 
      EQGSPVIDS LDAISFNPH SCRKDEANE RLTRKQLRL 
      ESIENLESY LDDKLRKQQ LIGKVVTCS SGIEKVHIR 
      RRLREVTFT WQRGIKIGQ GRFGKVYTA VNNKTGEMM 
      AVKELPLQH NDTHTIKRV GEEMKILEG IVHRNLVRY 
      YGVEIHKDE MLIFMEFCA EGTLETLVA ASENGLPEL 
      LVRRYTFQL VSGVAVLHD HGIVHRDIK TANIFLTEN 
      GNCLKIGDF GCAAKIKSH STMPGELQG FVGTQAYMA 
      PEVFTRNMS EGHGRAADI WSVGCVVVE MASGKRPWA 
      QFDSNYQIM FKVGMGQSP DPPDHMTDE GLDFLELCF 
      QHNPKDRAT AQELLDHSF VKLGDDFI
    CDS sequence
    • >TC000847 RA
      atggcctac agccccatc gagcaagac tgtgattta 
      agtgactac gatttgtac agtaaaacc ccaccacga 
      accaaaatt ttgcgtaaa aatcgcgaa cgcaagcag 
      aaacacaaa gatacaagc acgatgtcc tcacctaaa 
      gtcatcaaa cctaagccc ttaagtcgg cgtaacacc 
      atcagcagc atgttcgaa gagcttgtt gccaaggcc 
      gaaaatgac tcggaccaa ggctccaaa aggtccaac 
      aagcgccag ttgaaaatg ctgcgaggg tctgaacgt 
      gacctgaag ctggacatt gccagtgcc caagcggcc 
      tctgagaaa aaccaagaa ttcaaacac ccaaaaaca 
      ccacaacct agttatcag atcgagagc tgtaaaaga 
      ttcatgagc ttgtcttgt cggactgtt ccttgctcg 
      aaactgttg aagagtaaa aaagataat aaagtcaaa 
      tgtgacgaa attagcaat gaggagtgt tccgaaaat 
      cgagtcgat ttttacaac actttctca atgctaatt 
      cgaatgggc tgcggtgat aaaacgcag gaaatgagt 
      cacagacgc gctctgagc aaggaggag catttgtgg 
      caaaacgaa tttaaagat ttgatttgg cttgaattg 
      caagcgttc catgcggac cgaacccca agcgaagaa 
      gacagatac ttgtgttcc gaacgggaa aaagttgaa 
      cctctttta catgatata atgacttat cgctaccaa 
      aaaaattcg aaatgttac tcgacccaa agcagcgac 
      agtggcgtt gaggacgac tgttctggt tgtctttca 
      atctactgt ggttcgtgt cttgaggcc caaaacgaa 
      gcccttaaa gatgtggaa gaattgatg caaagatta 
      tcagcggtg gaagctttg ttcccttcc ggacacgct 
      tttgccgaa ctgtaccca ttatataac agtcctgag 
      ttcatagcg agggtcaag gctatcagt ttatggtac 
      aacatgacg cgacagcag agactgaaa ctaatgatt 
      cttgttaaa ttattagaa aataaagtg agtagagat 
      gatgataaa aacgggacg gaatcaaac agtcccagt 
      gatagcaat aacagttcc tcatcttcg gtaaatgag 
      tcgttctat gattgcaag tcaagtcag gatgctact 
      gttttttct tttgcgcct tttcccttc ttacatgtg 
      cataagtcg gaaagctcg gtctcacca tatcgaaaa 
      tacatcgaa aacattctt aaaacgcga tcgctccac 
      aaaagtttg agtttcttg gatcatctc caaatggtt 
      gttttgcga cgtgttcag ctaaccttg accaaaccg 
      gaaaccgac gatatttac aacaaagtg acttgtgaa 
      acggaagat gaagagttg caacgtttt gggtacttt 
      agccctgaa gccaaagcc ttgaacttg ccatcctat 
      cgtgcaatt tttctcttc cttgccgca attccgctc 
      aaaatgata cacgagttt ttgaagatg cgtttggag 
      caaaaacct gaaaaaccg tctcctttg agcgtccgc 
      caactgatg agggagtta aaagaaggc ttaaaagtc 
      gcaacgcaa caaaaagaa aagatcttg aagtacatt 
      gacggtgcg atctctgat agcaaaaaa ccgaacgat 
      gtgtttgaa gaaaagatg agggattat aacgagtgt 
      atgttgcgg gtgtttagt gactatttg gactatctg 
      gaacagtgg gccttgttg caccatgag actttccag 
      aagaatttg ttggaagaa gagtggcat ttttcgtgt 
      aatgtgatt aagtatatt ccgggcggg tcggaactt 
      ttagggaaa aagtttgtg tttattttg tgttcgatt 
      ttaaagtcg gtcgctgaa aggctgcac gatcggatc 
      gaggacgtg ctttgtaac atgcccaaa gacgacaaa 
      aatatgatc aagcaaacc gtgttcgtc atttgccgc 
      gaaatgcag tctctcttc aacgaagag cgcgaaatg 
      agcatcaaa accattgct ttttgcaag actgtcttt 
      cgccacgac cttcaccct gattgcaga tcagaacta 
      gtatgctca ataatttcc ctaaaatgt tcaataccc 
      gacgccata ggccgtgtc cagtccgaa ttcgatcaa 
      gtcaaacta gactcattt gaagaaatc gaacgaacg 
      gctatagtc tcccgcaca agggaaatc ttaatgcaa 
      gcctaccgt ttcggcttc gaattttac aaagaaatg 
      tccgatagc atccctacg gacaggcgc gagaaactc 
      gctagaagt atggtacaa tttgcgaat ttgtggatg 
      aaattcgtg actgaacga tgcgagagg gggcgaggg 
      atgagacca cggtgggct tatcaaggg ttggagttt 
      ctcttaact gtgtgtgaa cctcgaaac actaagcat 
      ttgacggaa gaagagttc gaagatctg aagaagaat 
      atggatgtt tgcataagt catgtgata ggaactacg 
      gctccttcg acgccggag tcagggttt tattcggcg 
      tcgccgagg agttcgatt gagcatata agggccagg 
      tcgaggggg tcgtccccg agtccgagg ccttcgtat 
      aaaagccaa agatcgaat agtcgcaaa actagcatg 
      gaacaaggg tcgccggtt attgatagt ttggatgct 
      attagtttt aatccacac agctgtaga aaggatgag 
      gcaaacgag cgattaact cggaagcag ctccgatta 
      gagtcaatc gaaaatctc gaaagttac cttgacgac 
      aaattgaga aagcagcaa ttaattggc aaagtggtg 
      acctgcagt agcggcatc gaaaaagtc cacatccgc 
      cgaagactg cgcgaggtg accttcacc tggcagagg 
      ggcataaaa atcgggcaa gggcgcttt ggcaaggtt 
      tacaccgct gttaataat aaaaccggc gaaatgatg 
      gcagtcaaa gaactaccg cttcagcat aacgatact 
      cataccatc aagcgagtg ggcgaagaa atgaaaatt 
      ttagaaggc attgttcat cgtaattta gtgagatat 
      tacggagtt gagattcat aaggatgaa atgctgata 
      ttcatggaa ttctgcgcc gaaggaact ctggaaact 
      ttagtggca gctagcgag aacggtctg cctgaactt 
      ttagttcga cggtacact tttcaatta gtttcggga 
      gtggccgtt ttgcacgac cacggaatc gtacataga 
      gacataaaa acggcgaat attttcctg actgagaac 
      ggcaactgc ttgaaaatt ggagatttt gggtgtgca 
      gctaaaatc aagtcgcat agtacaatg ccgggggaa 
      ttacaggga ttcgtaggg acgcaagcg tacatggca 
      cctgaagtg tttacacgc aacatgagc gaaggtcac 
      ggccgtgct gcggatata tggtcggtg ggttgtgtg 
      gtggtggaa atggcgtct gggaagcgg ccttgggca 
      cagtttgat tctaattac cagattatg ttcaaagtg 
      ggaatggga caaagcccg gatcctcct gatcatatg 
      acagacgaa ggattagat tttttggaa ttgtgtttc 
      cagcataat cccaaagac agggccacg gcccaagag 
      ctactagat cactcgttt gttaaactt ggtgatgat 
      tttatctaa
    mRNA sequence
    • >TC000847 RA
      tgccgaaag atgggttaa ttctgcgaa tctgtgttt 
      gtgaaacgt tccacattc gcaaaaaat gttcagtcg 
      atttactcg caattatgt gaaataaaa aacggacag 
      gtaaatcag ttcgcgctg ttttacgat actataaaa 
      tgatagagt gaaccatcg gttctgata ggttatgta 
      aacttagat aaaatgtgt gagtatttg tcattagtg 
      ttatcgagt taatttgtt taccgtaag agattaatt 
      acgatgagt gatcccaat agtacatac tttagtaaa 
      ttacggtaa ggggtgttg tattacgca attcaattg 
      tgtattaac caacacgta agaagttat ggcctacag 
      ccccatcga gcaagactg tgatttaag tgactacga 
      tttgtacag taaaacccc accacgaac caaaatttt 
      gcgtaaaaa tcgcgaacg caagcagaa acacaaaga 
      tacaagcac gatgtcctc acctaaagt catcaaacc 
      taagccctt aagtcggcg taacaccat cagcagcat 
      gttcgaaga gcttgttgc caaggccga aaatgactc 
      ggaccaagg ctccaaaag gtccaacaa gcgccagtt 
      gaaaatgct gcgagggtc tgaacgtga cctgaagct 
      ggacattgc cagtgccca agcggcctc tgagaaaaa 
      ccaagaatt caaacaccc aaaaacacc acaacctag 
      ttatcagat cgagagctg taaaagatt catgagctt 
      gtcttgtcg gactgttcc ttgctcgaa actgttgaa 
      gagtaaaaa agataataa agtcaaatg tgacgaaat 
      tagcaatga ggagtgttc cgaaaatcg agtcgattt 
      ttacaacac tttctcaat gctaattcg aatgggctg 
      cggtgataa aacgcagga aatgagtca cagacgcgc 
      tctgagcaa ggaggagca tttgtggca aaacgaatt 
      taaagattt gatttggct tgaattgca agcgttcca 
      tgcggaccg aaccccaag cgaagaaga cagatactt 
      gtgttccga acgggaaaa agttgaacc tcttttaca 
      tgatataat gacttatcg ctaccaaaa aaattcgaa 
      atgttactc gacccaaag cagcgacag tggcgttga 
      ggacgactg ttctggttg tctttcaat ctactgtgg 
      ttcgtgtct tgaggccca aaacgaagc ccttaaaga 
      tgtggaaga attgatgca aagattatc agcggtgga 
      agctttgtt cccttccgg acacgcttt tgccgaact 
      gtacccatt atataacag tcctgagtt catagcgag 
      ggtcaaggc tatcagttt atggtacaa catgacgcg 
      acagcagag actgaaact aatgattct tgttaaatt 
      attagaaaa taaagtgag tagagatga tgataaaaa 
      cgggacgga atcaaacag tcccagtga tagcaataa 
      cagttcctc atcttcggt aaatgagtc gttctatga 
      ttgcaagtc aagtcagga tgctactgt tttttcttt 
      tgcgccttt tcccttctt acatgtgca taagtcgga 
      aagctcggt ctcaccata tcgaaaata catcgaaaa 
      cattcttaa aacgcgatc gctccacaa aagtttgag 
      tttcttgga tcatctcca aatggttgt tttgcgacg 
      tgttcagct aaccttgac caaaccgga aaccgacga 
      tatttacaa caaagtgac ttgtgaaac ggaagatga 
      agagttgca acgttttgg gtactttag ccctgaagc 
      caaagcctt gaacttgcc atcctatcg tgcaatttt 
      tctcttcct tgccgcaat tccgctcaa aatgataca 
      cgagttttt gaagatgcg tttggagca aaaacctga 
      aaaaccgtc tcctttgag cgtccgcca actgatgag 
      ggagttaaa agaaggctt aaaagtcgc aacgcaaca 
      aaaagaaaa gatcttgaa gtacattga cggtgcgat 
      ctctgatag caaaaaacc gaacgatgt gtttgaaga 
      aaagatgag ggattataa cgagtgtat gttgcgggt 
      gtttagtga ctatttgga ctatctgga acagtgggc 
      cttgttgca ccatgagac tttccagaa gaatttgtt 
      ggaagaaga gtggcattt ttcgtgtaa tgtgattaa 
      gtatattcc gggcgggtc ggaactttt agggaaaaa 
      gtttgtgtt tattttgtg ttcgatttt aaagtcggt 
      cgctgaaag gctgcacga tcggatcga ggacgtgct 
      ttgtaacat gcccaaaga cgacaaaaa tatgatcaa 
      gcaaaccgt gttcgtcat ttgccgcga aatgcagtc 
      tctcttcaa cgaagagcg cgaaatgag catcaaaac 
      cattgcttt ttgcaagac tgtctttcg ccacgacct 
      tcaccctga ttgcagatc agaactagt atgctcaat 
      aatttccct aaaatgttc aatacccga cgccatagg 
      ccgtgtcca gtccgaatt cgatcaagt caaactaga 
      ctcatttga agaaatcga acgaacggc tatagtctc 
      ccgcacaag ggaaatctt aatgcaagc ctaccgttt 
      cggcttcga attttacaa agaaatgtc cgatagcat 
      ccctacgga caggcgcga gaaactcgc tagaagtat 
      ggtacaatt tgcgaattt gtggatgaa attcgtgac 
      tgaacgatg cgagagggg gcgagggat gagaccacg 
      gtgggctta tcaagggtt ggagtttct cttaactgt 
      gtgtgaacc tcgaaacac taagcattt gacggaaga 
      agagttcga agatctgaa gaagaatat ggatgtttg 
      cataagtca tgtgatagg aactacggc tccttcgac 
      gccggagtc agggtttta ttcggcgtc gccgaggag 
      ttcgattga gcatataag ggccaggtc gagggggtc 
      gtccccgag tccgaggcc ttcgtataa aagccaaag 
      atcgaatag tcgcaaaac tagcatgga acaagggtc 
      gccggttat tgatagttt ggatgctat tagttttaa 
      tccacacag ctgtagaaa ggatgaggc aaacgagcg 
      attaactcg gaagcagct ccgattaga gtcaatcga 
      aaatctcga aagttacct tgacgacaa attgagaaa 
      gcagcaatt aattggcaa agtggtgac ctgcagtag 
      cggcatcga aaaagtcca catccgccg aagactgcg 
      cgaggtgac cttcacctg gcagagggg cataaaaat 
      cgggcaagg gcgctttgg caaggttta caccgctgt 
      taataataa aaccggcga aatgatggc agtcaaaga 
      actaccgct tcagcataa cgatactca taccatcaa 
      gcgagtggg cgaagaaat gaaaatttt agaaggcat 
      tgttcatcg taatttagt gagatatta cggagttga 
      gattcataa ggatgaaat gctgatatt catggaatt 
      ctgcgccga aggaactct ggaaacttt agtggcagc 
      tagcgagaa cggtctgcc tgaactttt agttcgacg 
      gtacacttt tcaattagt ttcgggagt ggccgtttt 
      gcacgacca cggaatcgt acatagaga cataaaaac 
      ggcgaatat tttcctgac tgagaacgg caactgctt 
      gaaaattgg agattttgg gtgtgcagc taaaatcaa 
      gtcgcatag tacaatgcc gggggaatt acagggatt 
      cgtagggac gcaagcgta catggcacc tgaagtgtt 
      tacacgcaa catgagcga aggtcacgg ccgtgctgc 
      ggatatatg gtcggtggg ttgtgtggt ggtggaaat 
      ggcgtctgg gaagcggcc ttgggcaca gtttgattc 
      taattacca gattatgtt caaagtggg aatgggaca 
      aagcccgga tcctcctga tcatatgac agacgaagg 
      attagattt tttggaatt gtgtttcca gcataatcc 
      caaagacag ggccacggc ccaagagct actagatca 
      ctcgtttgt taaacttgg tgatgattt tatctaagt 
      attatgtac ctaattgtg ttaatttaa gtttttatg 
      taataatta attaatctc attattttt atacatatc 
      atttgattc tgttaaggc tgattgtgt ttttaaatg 
      tgtataaaa ttaccgttt gtgttttat ctagacagc 
      tgtctattt tatcgagtc gttgattga tagaatgtg 
      atattttac gcaccattg taggtggtg gactgtatg 
      gatcattgt tacaactat ccaaatatt attttgaat 
      aaacgataa gtttatta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY