| General information | |||
| TC000847 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 6735745..6746137 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC000847 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00180 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larval musculature differentiation incomplete - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC000847 RA
MAYSPIEQD CDLSDYDLY SKTPPRTKI LRKNRERKQ KHKDTSTMS SPKVIKPKP LSRRNTISS MFEELVAKA ENDSDQGSK RSNKRQLKM LRGSERDLK LDIASAQAA SEKNQEFKH PKTPQPSYQ IESCKRFMS LSCRTVPCS KLLKSKKDN KVKCDEISN EECSENRVD FYNTFSMLI RMGCGDKTQ EMSHRRALS KEEHLWQNE FKDLIWLEL QAFHADRTP SEEDRYLCS EREKVEPLL HDIMTYRYQ KNSKCYSTQ SSDSGVEDD CSGCLSIYC GSCLEAQNE ALKDVEELM QRLSAVEAL FPSGHAFAE LYPLYNSPE FIARVKAIS LWYNMTRQQ RLKLMILVK LLENKVSRD DDKNGTESN SPSDSNNSS SSSVNESFY DCKSSQDAT VFSFAPFPF LHVHKSESS VSPYRKYIE NILKTRSLH KSLSFLDHL QMVVLRRVQ LTLTKPETD DIYNKVTCE TEDEELQRF GYFSPEAKA LNLPSYRAI FLFLAAIPL KMIHEFLKM RLEQKPEKP SPLSVRQLM RELKEGLKV ATQQKEKIL KYIDGAISD SKKPNDVFE EKMRDYNEC MLRVFSDYL DYLEQWALL HHETFQKNL LEEEWHFSC NVIKYIPGG SELLGKKFV FILCSILKS VAERLHDRI EDVLCNMPK DDKNMIKQT VFVICREMQ SLFNEEREM SIKTIAFCK TVFRHDLHP DCRSELVCS IISLKCSIP DAIGRVQSE FDQVKLDSF EEIERTAIV SRTREILMQ AYRFGFEFY KEMSDSIPT DRREKLARS MVQFANLWM KFVTERCER GRGMRPRWA YQGLEFLLT VCEPRNTKH LTEEEFEDL KKNMDVCIS HVIGTTAPS TPESGFYSA SPRSSIEHI RARSRGSSP SPRPSYKSQ RSNSRKTSM EQGSPVIDS LDAISFNPH SCRKDEANE RLTRKQLRL ESIENLESY LDDKLRKQQ LIGKVVTCS SGIEKVHIR RRLREVTFT WQRGIKIGQ GRFGKVYTA VNNKTGEMM AVKELPLQH NDTHTIKRV GEEMKILEG IVHRNLVRY YGVEIHKDE MLIFMEFCA EGTLETLVA ASENGLPEL LVRRYTFQL VSGVAVLHD HGIVHRDIK TANIFLTEN GNCLKIGDF GCAAKIKSH STMPGELQG FVGTQAYMA PEVFTRNMS EGHGRAADI WSVGCVVVE MASGKRPWA QFDSNYQIM FKVGMGQSP DPPDHMTDE GLDFLELCF QHNPKDRAT AQELLDHSF VKLGDDFI
- >TC000847 RA
atggcctac agccccatc gagcaagac tgtgattta agtgactac gatttgtac agtaaaacc ccaccacga accaaaatt ttgcgtaaa aatcgcgaa cgcaagcag aaacacaaa gatacaagc acgatgtcc tcacctaaa gtcatcaaa cctaagccc ttaagtcgg cgtaacacc atcagcagc atgttcgaa gagcttgtt gccaaggcc gaaaatgac tcggaccaa ggctccaaa aggtccaac aagcgccag ttgaaaatg ctgcgaggg tctgaacgt gacctgaag ctggacatt gccagtgcc caagcggcc tctgagaaa aaccaagaa ttcaaacac ccaaaaaca ccacaacct agttatcag atcgagagc tgtaaaaga ttcatgagc ttgtcttgt cggactgtt ccttgctcg aaactgttg aagagtaaa aaagataat aaagtcaaa tgtgacgaa attagcaat gaggagtgt tccgaaaat cgagtcgat ttttacaac actttctca atgctaatt cgaatgggc tgcggtgat aaaacgcag gaaatgagt cacagacgc gctctgagc aaggaggag catttgtgg caaaacgaa tttaaagat ttgatttgg cttgaattg caagcgttc catgcggac cgaacccca agcgaagaa gacagatac ttgtgttcc gaacgggaa aaagttgaa cctctttta catgatata atgacttat cgctaccaa aaaaattcg aaatgttac tcgacccaa agcagcgac agtggcgtt gaggacgac tgttctggt tgtctttca atctactgt ggttcgtgt cttgaggcc caaaacgaa gcccttaaa gatgtggaa gaattgatg caaagatta tcagcggtg gaagctttg ttcccttcc ggacacgct tttgccgaa ctgtaccca ttatataac agtcctgag ttcatagcg agggtcaag gctatcagt ttatggtac aacatgacg cgacagcag agactgaaa ctaatgatt cttgttaaa ttattagaa aataaagtg agtagagat gatgataaa aacgggacg gaatcaaac agtcccagt gatagcaat aacagttcc tcatcttcg gtaaatgag tcgttctat gattgcaag tcaagtcag gatgctact gttttttct tttgcgcct tttcccttc ttacatgtg cataagtcg gaaagctcg gtctcacca tatcgaaaa tacatcgaa aacattctt aaaacgcga tcgctccac aaaagtttg agtttcttg gatcatctc caaatggtt gttttgcga cgtgttcag ctaaccttg accaaaccg gaaaccgac gatatttac aacaaagtg acttgtgaa acggaagat gaagagttg caacgtttt gggtacttt agccctgaa gccaaagcc ttgaacttg ccatcctat cgtgcaatt tttctcttc cttgccgca attccgctc aaaatgata cacgagttt ttgaagatg cgtttggag caaaaacct gaaaaaccg tctcctttg agcgtccgc caactgatg agggagtta aaagaaggc ttaaaagtc gcaacgcaa caaaaagaa aagatcttg aagtacatt gacggtgcg atctctgat agcaaaaaa ccgaacgat gtgtttgaa gaaaagatg agggattat aacgagtgt atgttgcgg gtgtttagt gactatttg gactatctg gaacagtgg gccttgttg caccatgag actttccag aagaatttg ttggaagaa gagtggcat ttttcgtgt aatgtgatt aagtatatt ccgggcggg tcggaactt ttagggaaa aagtttgtg tttattttg tgttcgatt ttaaagtcg gtcgctgaa aggctgcac gatcggatc gaggacgtg ctttgtaac atgcccaaa gacgacaaa aatatgatc aagcaaacc gtgttcgtc atttgccgc gaaatgcag tctctcttc aacgaagag cgcgaaatg agcatcaaa accattgct ttttgcaag actgtcttt cgccacgac cttcaccct gattgcaga tcagaacta gtatgctca ataatttcc ctaaaatgt tcaataccc gacgccata ggccgtgtc cagtccgaa ttcgatcaa gtcaaacta gactcattt gaagaaatc gaacgaacg gctatagtc tcccgcaca agggaaatc ttaatgcaa gcctaccgt ttcggcttc gaattttac aaagaaatg tccgatagc atccctacg gacaggcgc gagaaactc gctagaagt atggtacaa tttgcgaat ttgtggatg aaattcgtg actgaacga tgcgagagg gggcgaggg atgagacca cggtgggct tatcaaggg ttggagttt ctcttaact gtgtgtgaa cctcgaaac actaagcat ttgacggaa gaagagttc gaagatctg aagaagaat atggatgtt tgcataagt catgtgata ggaactacg gctccttcg acgccggag tcagggttt tattcggcg tcgccgagg agttcgatt gagcatata agggccagg tcgaggggg tcgtccccg agtccgagg ccttcgtat aaaagccaa agatcgaat agtcgcaaa actagcatg gaacaaggg tcgccggtt attgatagt ttggatgct attagtttt aatccacac agctgtaga aaggatgag gcaaacgag cgattaact cggaagcag ctccgatta gagtcaatc gaaaatctc gaaagttac cttgacgac aaattgaga aagcagcaa ttaattggc aaagtggtg acctgcagt agcggcatc gaaaaagtc cacatccgc cgaagactg cgcgaggtg accttcacc tggcagagg ggcataaaa atcgggcaa gggcgcttt ggcaaggtt tacaccgct gttaataat aaaaccggc gaaatgatg gcagtcaaa gaactaccg cttcagcat aacgatact cataccatc aagcgagtg ggcgaagaa atgaaaatt ttagaaggc attgttcat cgtaattta gtgagatat tacggagtt gagattcat aaggatgaa atgctgata ttcatggaa ttctgcgcc gaaggaact ctggaaact ttagtggca gctagcgag aacggtctg cctgaactt ttagttcga cggtacact tttcaatta gtttcggga gtggccgtt ttgcacgac cacggaatc gtacataga gacataaaa acggcgaat attttcctg actgagaac ggcaactgc ttgaaaatt ggagatttt gggtgtgca gctaaaatc aagtcgcat agtacaatg ccgggggaa ttacaggga ttcgtaggg acgcaagcg tacatggca cctgaagtg tttacacgc aacatgagc gaaggtcac ggccgtgct gcggatata tggtcggtg ggttgtgtg gtggtggaa atggcgtct gggaagcgg ccttgggca cagtttgat tctaattac cagattatg ttcaaagtg ggaatggga caaagcccg gatcctcct gatcatatg acagacgaa ggattagat tttttggaa ttgtgtttc cagcataat cccaaagac agggccacg gcccaagag ctactagat cactcgttt gttaaactt ggtgatgat tttatctaa
- >TC000847 RA
tgccgaaag atgggttaa ttctgcgaa tctgtgttt gtgaaacgt tccacattc gcaaaaaat gttcagtcg atttactcg caattatgt gaaataaaa aacggacag gtaaatcag ttcgcgctg ttttacgat actataaaa tgatagagt gaaccatcg gttctgata ggttatgta aacttagat aaaatgtgt gagtatttg tcattagtg ttatcgagt taatttgtt taccgtaag agattaatt acgatgagt gatcccaat agtacatac tttagtaaa ttacggtaa ggggtgttg tattacgca attcaattg tgtattaac caacacgta agaagttat ggcctacag ccccatcga gcaagactg tgatttaag tgactacga tttgtacag taaaacccc accacgaac caaaatttt gcgtaaaaa tcgcgaacg caagcagaa acacaaaga tacaagcac gatgtcctc acctaaagt catcaaacc taagccctt aagtcggcg taacaccat cagcagcat gttcgaaga gcttgttgc caaggccga aaatgactc ggaccaagg ctccaaaag gtccaacaa gcgccagtt gaaaatgct gcgagggtc tgaacgtga cctgaagct ggacattgc cagtgccca agcggcctc tgagaaaaa ccaagaatt caaacaccc aaaaacacc acaacctag ttatcagat cgagagctg taaaagatt catgagctt gtcttgtcg gactgttcc ttgctcgaa actgttgaa gagtaaaaa agataataa agtcaaatg tgacgaaat tagcaatga ggagtgttc cgaaaatcg agtcgattt ttacaacac tttctcaat gctaattcg aatgggctg cggtgataa aacgcagga aatgagtca cagacgcgc tctgagcaa ggaggagca tttgtggca aaacgaatt taaagattt gatttggct tgaattgca agcgttcca tgcggaccg aaccccaag cgaagaaga cagatactt gtgttccga acgggaaaa agttgaacc tcttttaca tgatataat gacttatcg ctaccaaaa aaattcgaa atgttactc gacccaaag cagcgacag tggcgttga ggacgactg ttctggttg tctttcaat ctactgtgg ttcgtgtct tgaggccca aaacgaagc ccttaaaga tgtggaaga attgatgca aagattatc agcggtgga agctttgtt cccttccgg acacgcttt tgccgaact gtacccatt atataacag tcctgagtt catagcgag ggtcaaggc tatcagttt atggtacaa catgacgcg acagcagag actgaaact aatgattct tgttaaatt attagaaaa taaagtgag tagagatga tgataaaaa cgggacgga atcaaacag tcccagtga tagcaataa cagttcctc atcttcggt aaatgagtc gttctatga ttgcaagtc aagtcagga tgctactgt tttttcttt tgcgccttt tcccttctt acatgtgca taagtcgga aagctcggt ctcaccata tcgaaaata catcgaaaa cattcttaa aacgcgatc gctccacaa aagtttgag tttcttgga tcatctcca aatggttgt tttgcgacg tgttcagct aaccttgac caaaccgga aaccgacga tatttacaa caaagtgac ttgtgaaac ggaagatga agagttgca acgttttgg gtactttag ccctgaagc caaagcctt gaacttgcc atcctatcg tgcaatttt tctcttcct tgccgcaat tccgctcaa aatgataca cgagttttt gaagatgcg tttggagca aaaacctga aaaaccgtc tcctttgag cgtccgcca actgatgag ggagttaaa agaaggctt aaaagtcgc aacgcaaca aaaagaaaa gatcttgaa gtacattga cggtgcgat ctctgatag caaaaaacc gaacgatgt gtttgaaga aaagatgag ggattataa cgagtgtat gttgcgggt gtttagtga ctatttgga ctatctgga acagtgggc cttgttgca ccatgagac tttccagaa gaatttgtt ggaagaaga gtggcattt ttcgtgtaa tgtgattaa gtatattcc gggcgggtc ggaactttt agggaaaaa gtttgtgtt tattttgtg ttcgatttt aaagtcggt cgctgaaag gctgcacga tcggatcga ggacgtgct ttgtaacat gcccaaaga cgacaaaaa tatgatcaa gcaaaccgt gttcgtcat ttgccgcga aatgcagtc tctcttcaa cgaagagcg cgaaatgag catcaaaac cattgcttt ttgcaagac tgtctttcg ccacgacct tcaccctga ttgcagatc agaactagt atgctcaat aatttccct aaaatgttc aatacccga cgccatagg ccgtgtcca gtccgaatt cgatcaagt caaactaga ctcatttga agaaatcga acgaacggc tatagtctc ccgcacaag ggaaatctt aatgcaagc ctaccgttt cggcttcga attttacaa agaaatgtc cgatagcat ccctacgga caggcgcga gaaactcgc tagaagtat ggtacaatt tgcgaattt gtggatgaa attcgtgac tgaacgatg cgagagggg gcgagggat gagaccacg gtgggctta tcaagggtt ggagtttct cttaactgt gtgtgaacc tcgaaacac taagcattt gacggaaga agagttcga agatctgaa gaagaatat ggatgtttg cataagtca tgtgatagg aactacggc tccttcgac gccggagtc agggtttta ttcggcgtc gccgaggag ttcgattga gcatataag ggccaggtc gagggggtc gtccccgag tccgaggcc ttcgtataa aagccaaag atcgaatag tcgcaaaac tagcatgga acaagggtc gccggttat tgatagttt ggatgctat tagttttaa tccacacag ctgtagaaa ggatgaggc aaacgagcg attaactcg gaagcagct ccgattaga gtcaatcga aaatctcga aagttacct tgacgacaa attgagaaa gcagcaatt aattggcaa agtggtgac ctgcagtag cggcatcga aaaagtcca catccgccg aagactgcg cgaggtgac cttcacctg gcagagggg cataaaaat cgggcaagg gcgctttgg caaggttta caccgctgt taataataa aaccggcga aatgatggc agtcaaaga actaccgct tcagcataa cgatactca taccatcaa gcgagtggg cgaagaaat gaaaatttt agaaggcat tgttcatcg taatttagt gagatatta cggagttga gattcataa ggatgaaat gctgatatt catggaatt ctgcgccga aggaactct ggaaacttt agtggcagc tagcgagaa cggtctgcc tgaactttt agttcgacg gtacacttt tcaattagt ttcgggagt ggccgtttt gcacgacca cggaatcgt acatagaga cataaaaac ggcgaatat tttcctgac tgagaacgg caactgctt gaaaattgg agattttgg gtgtgcagc taaaatcaa gtcgcatag tacaatgcc gggggaatt acagggatt cgtagggac gcaagcgta catggcacc tgaagtgtt tacacgcaa catgagcga aggtcacgg ccgtgctgc ggatatatg gtcggtggg ttgtgtggt ggtggaaat ggcgtctgg gaagcggcc ttgggcaca gtttgattc taattacca gattatgtt caaagtggg aatgggaca aagcccgga tcctcctga tcatatgac agacgaagg attagattt tttggaatt gtgtttcca gcataatcc caaagacag ggccacggc ccaagagct actagatca ctcgtttgt taaacttgg tgatgattt tatctaagt attatgtac ctaattgtg ttaatttaa gtttttatg taataatta attaatctc attattttt atacatatc atttgattc tgttaaggc tgattgtgt ttttaaatg tgtataaaa ttaccgttt gtgttttat ctagacagc tgtctattt tatcgagtc gttgattga tagaatgtg atattttac gcaccattg taggtggtg gactgtatg gatcattgt tacaactat ccaaatatt attttgaat aaacgataa gtttatta
