Status
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Gene information
General information
TC000840 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6615207..6619547
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000840
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00179

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000840 RA
      MRHLEQEVS SSDQCKAEY LDNFLEIKE EIPNLILDE 
      CENFMKNEF TSDEESGSN LHLDLNNDS ESDEDDTAV 
      KKRRNRRKS LVPQKAVQL EVSQNGSMF SGSESSQDL 
      IEKTQSLCS SDTNIDKFL LSDNLNALE MPDNDDLLN 
      INEEDIFKD LQEEADGEK LDDEILDNV KEEWRAHWG 
      VKCNMCEQV FPYKTEFDK HYQDNYGLI PVYTCTFCN 
      KTAEKYSTF RSHCYRHIT EGRYKCQAC PKGFSLQSM 
      LHVHVLAKH TKAKPFKCD ECGKSFVTK PGLKIHLRK 
      HKTELKEDY PCVECGKIL HTRGGLTSH MNVHRLGRR 
      FMCDVCGKT FTQKVNMQQ HVKQHTGDK PHGCDKCGK 
      TFAEKSHLA RHYSFHSDQ RPFKCDVCQ KMYKTERCL 
      KVHSLVHAA ARPFVCSYC SKGFLSSTK LKQHFNIHT 
      GERPYKCKY CERTFTNYP NWLKHTRRR HKVDHKTGE 
      NLGPKVPKE ETSQSTVIE TTDTSEQLV ADISLSKTE 
      ELLLQQGLL NFPLTDDKF VIPQQIDFL AGTSNNSVG 
      VIVPYFQTS LPNHQTLIQ GSNNVMTMQ FSDLVHSQY 
      LQQPLM
    CDS sequence
    • >TC000840 RA
      atgagacac ctcgagcaa gaagtttca agctcggat 
      cagtgtaaa gcggagtat ttggataac ttccttgag 
      ataaaggag gaaattccg aacttaatc ttagacgaa 
      tgcgagaat ttcatgaaa aatgagttt acgagtgac 
      gaggagagt ggtagtaat ttgcactta gatttgaac 
      aatgatagt gagagtgat gaggatgat acagcggtt 
      aaaaaacgc cgaaaccgg cgaaaatcg cttgtgcca 
      cagaaagct gtccagttg gaagttagc cagaacggt 
      tcaatgttc agtggttcc gaatcctct caggatttg 
      atagagaag acacaaagt ctttgctct tcagacact 
      aacattgat aaattcctc ctatcagat aacttgaat 
      gccttagaa atgccagat aacgacgat ttattaaac 
      ataaacgag gaagacatt ttcaaagac ttgcaagag 
      gaagctgat ggtgagaaa ttagacgat gaaattttg 
      gacaatgtg aaggaggaa tggagggct cattggggg 
      gtcaaatgt aacatgtgc gagcaagtc tttccctac 
      aaaactgaa tttgataaa cactaccaa gacaattat 
      ggcttaatt cccgtgtat acttgcact ttttgcaac 
      aagactgct gaaaaatat tccaccttt aggtctcac 
      tgttacagg catataaca gaagggcgc tacaagtgt 
      caagcgtgt cctaaaggt tttagttta caaagcatg 
      ttacacgtc catgtgtta gcaaagcac acgaaagct 
      aagcctttt aagtgtgat gagtgtggt aagagcttc 
      gttactaaa ccaggactt aaaattcat cttagaaaa 
      cacaagact gaactcaag gaggattac ccgtgtgtg 
      gagtgtggt aaaatatta cacactcga ggcggttta 
      acctctcac atgaacgtc caccggtta ggccgccgt 
      ttcatgtgt gacgtttgc ggtaaaacg ttcacccaa 
      aaagtgaac atgcaacaa cacgtcaaa caacacact 
      ggtgataaa ccccacggt tgtgataaa tgtggcaaa 
      acttttgca gaaaaatca cacttggcg cgccattac 
      agcttccac agcgaccag cggccgttt aagtgtgat 
      gtgtgccaa aaaatgtac aagactgaa cgttgttta 
      aaagtccat agtttagta catgctgcg gccagaccc 
      ttcgtttgt agttattgc agtaagggc tttttgagt 
      agcaccaaa ttgaagcaa cactttaat attcataca 
      ggtgaacgg ccttacaag tgtaaatat tgcgaacgc 
      acatttacg aattatcca aattggctg aaacacaca 
      cgaagacga cataaagtt gatcacaag actggagaa 
      aatttggga cctaaagta cctaaagag gaaacatca 
      caatcgact gtgatcgaa actactgat acgtcagag 
      caattagta gcggatatt tcgcttagt aaaactgaa 
      gagcttctc ctacagcag ggacttttg aattttcca 
      ctcacagac gataagttt gtcattccg caacaaatc 
      gatttttta gccggtact agtaataac tcagtgggg 
      gttatcgtt ccatacttc caaacatca ctaccaaat 
      catcaaacc ttaatccaa ggttctaat aacgtaatg 
      accatgcaa ttcagcgat ttggtccac tctcagtat 
      ttacaacaa ccgttgatg taa
    mRNA sequence
    • >TC000840 RA
      ccttggtga aaaacgaaa gtttctagg ggtgcgtga 
      catggtggt ggtggtgaa tttttttgc ttaatttta 
      ataatgtct tatctgcaa caattaaaa cccaattca 
      ataaaaaat tagcgaaaa cagtgccaa tgtgtggga 
      gagttgtgt taaatttcg gtttttaaa aatgtgagc 
      accctaatt tgtttactt tgtggtatt tataatcga 
      tacgggatt cagaggtga cgccaccta acctaactt 
      taatttgta aacataaaa atgagacac ctcgagcaa 
      gaagtttca agctcggat cagtgtaaa gcggagtat 
      ttggataac ttccttgag ataaaggag gaaattccg 
      aacttaatc ttagacgaa tgcgagaat ttcatgaaa 
      aatgagttt acgagtgac gaggagagt ggtagtaat 
      ttgcactta gatttgaac aatgatagt gagagtgat 
      gaggatgat acagcggtt aaaaaacgc cgaaaccgg 
      cgaaaatcg cttgtgcca cagaaagct gtccagttg 
      gaagttagc cagaacggt tcaatgttc agtggttcc 
      gaatcctct caggatttg atagagaag acacaaagt 
      ctttgctct tcagacact aacattgat aaattcctc 
      ctatcagat aacttgaat gccttagaa atgccagat 
      aacgacgat ttattaaac ataaacgag gaagacatt 
      ttcaaagac ttgcaagag gaagctgat ggtgagaaa 
      ttagacgat gaaattttg gacaatgtg aaggaggaa 
      tggagggct cattggggg gtcaaatgt aacatgtgc 
      gagcaagtc tttccctac aaaactgaa tttgataaa 
      cactaccaa gacaattat ggcttaatt cccgtgtat 
      acttgcact ttttgcaac aagactgct gaaaaatat 
      tccaccttt aggtctcac tgttacagg catataaca 
      gaagggcgc tacaagtgt caagcgtgt cctaaaggt 
      tttagttta caaagcatg ttacacgtc catgtgtta 
      gcaaagcac acgaaagct aagcctttt aagtgtgat 
      gagtgtggt aagagcttc gttactaaa ccaggactt 
      aaaattcat cttagaaaa cacaagact gaactcaag 
      gaggattac ccgtgtgtg gagtgtggt aaaatatta 
      cacactcga ggcggttta acctctcac atgaacgtc 
      caccggtta ggccgccgt ttcatgtgt gacgtttgc 
      ggtaaaacg ttcacccaa aaagtgaac atgcaacaa 
      cacgtcaaa caacacact ggtgataaa ccccacggt 
      tgtgataaa tgtggcaaa acttttgca gaaaaatca 
      cacttggcg cgccattac agcttccac agcgaccag 
      cggccgttt aagtgtgat gtgtgccaa aaaatgtac 
      aagactgaa cgttgttta aaagtccat agtttagta 
      catgctgcg gccagaccc ttcgtttgt agttattgc 
      agtaagggc tttttgagt agcaccaaa ttgaagcaa 
      cactttaat attcataca ggtgaacgg ccttacaag 
      tgtaaatat tgcgaacgc acatttacg aattatcca 
      aattggctg aaacacaca cgaagacga cataaagtt 
      gatcacaag actggagaa aatttggga cctaaagta 
      cctaaagag gaaacatca caatcgact gtgatcgaa 
      actactgat acgtcagag caattagta gcggatatt 
      tcgcttagt aaaactgaa gagcttctc ctacagcag 
      ggacttttg aattttcca ctcacagac gataagttt 
      gtcattccg caacaaatc gatttttta gccggtact 
      agtaataac tcagtgggg gttatcgtt ccatacttc 
      caaacatca ctaccaaat catcaaacc ttaatccaa 
      ggttctaat aacgtaatg accatgcaa ttcagcgat 
      ttggtccac tctcagtat ttacaacaa ccgttgatg 
      taattttac aaaaacaat caaagcgca acattattt 
      tattttatt attttttac cctcctcgg cagcttttt 
      cacgttttt acaccctat tttttattt ttattatac 
      catgcctac aaataaatt tctgacttc tgggtataa 
      attcgcttg gcaacatta attttcaga tataaatga 
      catttgtca tttatttct gatgagtga ctgctaaca 
      tgactgttt aatatcgcc aataatacg atgttgcca 
      acttaattt catacttaa gctgaaaac tgcagactc 
      tggtcaaaa tatgaaatg ccaatttta tttgatatt 
      tcatttctc gctatttcg caaaagagc gattcttta 
      cattacttt acatgcgag taaccgaaa agatatagc 
      caatttaat ttttaatta ttttattgt gatctttgt 
      tgtaaataa tccgctgag tattatttt acaaatcta 
      cagtattta agataaaat acttgataa ttttggggt 
      gttgagaaa catttgaaa acaccctgt atttgcagt 
      atgtgtcaa cattccaca atttatcaa ttcgtatca 
      atgtgtatg tttttagac ttaaaacgt agtattaaa 
      ctgagtatt cttaaataa gtattcgtt atattttgt 
      atttttata cttgagaac gtgtcgtaa agctgtaag 
      acttgaatt tattttaaa tgttttaat tgtatgtaa 
      ctgtattca cctaaaaca ggcaataaa atgtgatac 
      aaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY