General information | |||
TC000831 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 6419036..6428190 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC000831 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00178 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
head irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
head bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
head pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
thorax pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
abdomen pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 42.86% (includes death as larva, prepupa, pupa, adult)
Comment: ectopic, median, ap-oriented groove |
- >TC000831 RA
MAIFTNVVQ EFREEIEAN LTLDDVIDT LIAQKIIDE KLYIEIWGN QHNRVKYLL DIIVKDGKR CSTFIQILG TVARMKHIS ESISSSIEK IRKVIVIGG FPEFSLNYV VRNSVANTI KDRALGLDP KLGGILVVY GLPGFGKSC LVNQVLQTK RFLEVSGNA MFWINLGEY SDTTAIINP LWNLYLTAV KLTDYVALS HRPDDIIIL QNELKRLFL HENLANSYI ILDDVKSPD VLNAFKLSC KTIVTTQDK NVANNSEAF FIKVTTGFT KKESIELLR KSLETDNLA NRENLANRI HELCDGCPM LLNIIGSIL KDSREEALS TDIIWKSLI DEIITNDYL QHPTTMKVI HMIQRFVTC LDPEVQRCF HSLAVFPKH VNIRPEILM KLWDKTYYE VRQIMVELE NKSLIVSFY NKDLKSYIY SIHSLVRTY LVEKAKIDI KYYHEKLIL QCDEIIDKS PPDDPIANY IFGYYGYHA KNAQLYHKF EKYFDLRFI ENKIKRVGS ADLLTDFEI YRDFITKKE PHLERKLKD YIKFVHSYS AHLHISPET NIVQFAFFN DNNYVYEDA KHIASNNPD QLYFKLEPY SGGHKNFPV LKTKSVATA TCFVDSKEV LVGTASGSI EVKSFRQYQ HKVHRFEGH SDQVFSLQI SPDSKSFLS ISVDHTAKL WRFVEELDP QSPKLKQTN WKNLHSPDE NAIPHKTFK IQDGDYITS AAFPYDTCD EIITASNKG KIMRWNVRT ASIVRESSA LGLPISRIC YVHDSEQVI FAYDGKVYC YARGNLVYN GQFGVLDDI TDLVLIPIV SEYREVAVI SGRKLEIWE YVKKVKRFS FEAQSNITA TVINDEYLV LGTAEYIYL YDYRCQKID QYTNDHGEP TSLYILRES IAFICLDKE NVQRCELYF EPCSKPLVK DLALFNCYW KESQPIYFI VNSANKIDI FYGHSTILA GIDEITDKI ISCSFSLCG NYLILGTES GKLYFYNYK KKKVTCLKI CDEEIKLVK CFEFDEVVD YDSVSACGM VVVASSSKI VIIVDNRVK LELDILDEL TDVLYLRNR LIAFDVKGR FSIACLSDD QCLGWISTK YKDVAIARI HKGKNLLAI ACKEGNGYV LRVLTFVSD NPLTLQCLF EAKLNTTPT CMSLNSNGK LVSVGGDNG DIEVFSIEN NQQDLLLLH KKSVEQLVF APNFESILV SLGDQIAWW NLNDFGKKS EKMNGNSEH SHQSLVFNF DPWQDKKLH DTVPYLLTS TNLHGKRLQ YISASKDFR SFLIIDIDG NIFKMDIID S
- >TC000831 RA
atggcaatc ttcacaaat gtggtacaa gagtttcgg gaagaaatt gaagctaac ctgaccctc gacgatgta attgatacg ttaattgca cagaagatc atcgacgaa aaactctac attgaaata tggggcaat caacataac cgcgtaaag tacttattg gatatcatc gtcaaagat ggcaagcgg tgctccaca ttcattcaa attctggga actgtggct cgcatgaag cacatttcc gaatcgatc agttccagc attgaaaaa atacgaaaa gttattgtg attggcggt tttcccgaa ttttccttg aattatgtt gtgagaaat tcagtggct aataccatc aaggatagg gcgctaggt ctcgaccct aaacttggc ggtattttg gtagtgtat ggtttgccc ggttttggt aaatcatgt ttagtaaat caagttttg caaactaaa cgctttttg gaagtgtct ggaaatgcc atgttttgg atcaattta ggagaatat tcagacacc actgccatt attaatccc ttatggaat ttgtatttg actgctgtt aagctaact gattatgta gctttaagt cataggcca gatgacatc attattttg caaaatgaa ctgaaaaga ttgtttttg cacgaaaat ttagccaat tcgtatatc attcttgat gatgtcaaa tcgccagat gttctaaat gcttttaaa ttaagttgt aaaactatt gtgaccacg caggataaa aatgtggcc aacaattcg gaggcattt ttcattaag gttacgaca ggttttact aaaaaagaa tcaatcgaa ttgttacga aaatctcta gaaactgac aatctcgca aatagggaa aatctagca aatcgaata cacgagttg tgcgatggt tgtcccatg ttgttaaat atcataggt tcaatttta aaagatagc cgcgaagaa gccttgtct actgatatc atctggaaa tcactaatc gatgaaatt atcacaaac gattacttg caacaccca acaacaatg aaagtgatt cacatgatc caaagattc gttacctgc ttagacccc gaagttcaa aggtgcttc cacagcttg gctgttttt cccaaacat gtcaacatt cgaccggaa atactcatg aaattgtgg gacaagacg tattatgaa gtgagacaa ataatggta gagttagaa aacaagtct ctcatcgtt tccttttac aacaaagat ctcaaatcg tacatatac agtatccat agtttagtt cgcacgtat ttagttgaa aaagcgaaa atcgacatt aaatactac cacgaaaaa ctgatcttg caatgtgat gaaatcatc gacaagtca ccgcctgat gaccctatt gctaactac atttttggc tattacggg tatcatgcc aaaaacgcg caactttac cacaaattc gaaaaatat ttcgattta agattcatt gagaacaaa ataaaacgg gtcggatcg gctgatctc ctgactgat ttcgaaata tacagggat ttcatcaca aagaaggaa cctcacttg gagcgcaaa ctgaaagac tacataaag tttgtgcac tcatacagc gcccatttg cacatttct cccgagaca aatatcgtg cagtttgcg tttttcaat gacaataat tacgtctac gaagatgca aaacatatc gccagcaat aatcccgac caactgtac ttcaaattg gagccgtat tctggtgga cataagaat tttccagtt ttaaagacg aaaagtgtg gcaactgct acgtgtttt gtcgattcg aaggaggtt ctcgtgggc acagctagc ggcagcatt gaagtcaag tcgttccgt caataccag cacaaagtc caccgcttt gaaggccac tctgaccaa gtcttcagc ttgcaaata agtcccgat tcaaaaagc tttctctca atttccgtc gaccacact gccaaactg tggcgattt gtcgaagaa ctcgaccct caatcgccc aaactgaaa caaaccaat tggaaaaac ttacattcc cctgatgaa aatgcaatc ccacacaaa actttcaaa attcaagac ggcgactac atcacatct gcggctttc ccttatgat acatgcgat gagataatt acggcttcg aataaagga aaaattatg agatggaat gtgagaact gcgtcaatt gtgagggaa tcatcggct ttaggattg ccaatttcg cggatttgc tatgttcac gatagtgag caggtgatt tttgcgtac gatggcaag gtgtattgc tatgccagg gggaatttg gtttataat ggacagttt ggggttttg gatgatatt accgatttg gttcttatt ccgattgtt tcggagtat agggaagtg gctgtgatt agtggaagg aagcttgag atctgggaa tatgtgaag aaagtcaag agatttagt tttgaagcc caatcgaat attacagct actgtgatc aatgacgaa tacttggtt ttgggcacg gctgagtac atttatttg tacgattat aggtgtcag aaaattgat caatacaca aacgatcat ggggaaccc acctcactt tacatcctt cgggaaagc atcgctttc atttgtctc gataaggaa aacgtacaa cgatgcgaa ttatacttc gaaccctgc agtaaacct ttagtcaaa gacctggct ctcttcaat tgctactgg aaagagtca caaccgatt tatttcatc gttaatagt gcaaataaa atcgatata ttctacggt cattcaaca atcctggca ggaattgat gaaatcacc gataaaatt atttcgtgt tcattctcc ctctgtgga aattacctg attttgggg acggagtcg gggaagttg tacttttac aattacaag aagaagaaa gtcacttgt ttgaaaatc tgcgatgag gaaataaaa ttggttaag tgcttcgaa ttcgatgaa gttgttgat tatgacagt gttagtgct tgcggaatg gttgtggtt gcttctagt tcaaaaatc gtgataata gttgataat agagtgaaa ttggagctt gatattttg gatgaattg accgacgtg ttgtacctt cggaaccgc ttgattgcg tttgatgtc aaaggccgg ttttcaatc gcctgctta agtgacgat caatgttta ggctggatt tccacaaag tataaagac gtggctatt gctagaatt cacaaaggg aaaaatctg ctagcgatt gcctgcaag gaagggaat ggttacgtt ttgcgagtc cttacattc gtttctgat aatcctttg actctgcaa tgtttattt gaggcgaag ttgaacacg actccgact tgcatgagc ttgaattcc aacggcaaa ctcgtctca gttggtggt gataatggg gacatagag gtgttttcg atcgaaaac aaccaacaa gacttgctg cttttgcat aaaaagtca gtcgaacag cttgttttc gctcccaat ttcgagtcg attcttgtg agtttgggg gatcagatc gcttggtgg aacttgaat gattttgga aaaaaaagt gagaaaatg aatggaaat tctgagcat tcccaccag tctttggtg ttcaatttt gatccatgg caagacaag aaacttcat gacactgtg ccttatttg ctcactagt acaaattta cacggaaag aggttgcag tacatttct gcttcgaaa gattttcga tcgtttttg atcatcgac atcgacggt aatatcttc aaaatggat attatcgat tcctga
- >TC000831 RA
ttggcggaa gatttgaca tttgacatt gttttgttg ttggtttaa caataaata atccaattg gctagttta cctgcaagg tttctattg ttaattaga agtccacct atacaaaag aattcaaac gaaataacc aaattcaag gccgttgag taattcgga cgtctcata taatttgtt tgtgtggtt gtggtcaat gaccgcacg aaagatttg acgaataga catgcagaa gattctgag tcgtacagg ttttgacag gtatttttg agtgtgctt agagtagtg tcgttttgt gtttgctca aaaggcgtg atatcgtca cttttgata acaaatcga tcataccaa cgatttttc ctttattca ttacggaaa aaatactgt ctttaatgc taccccgat ggcaatctt cacaaatgt ggtacaaga gtttcggga agaaattga agctaacct gaccctcga cgatgtaat tgatacgtt aattgcaca gaagatcat cgacgaaaa actctacat tgaaatatg gggcaatca acataaccg cgtaaagta cttattgga tatcatcgt caaagatgg caagcggtg ctccacatt cattcaaat tctgggaac tgtggctcg catgaagca catttccga atcgatcag ttccagcat tgaaaaaat acgaaaagt tattgtgat tggcggttt tcccgaatt ttccttgaa ttatgttgt gagaaattc agtggctaa taccatcaa ggatagggc gctaggtct cgaccctaa acttggcgg tattttggt agtgtatgg tttgcccgg ttttggtaa atcatgttt agtaaatca agttttgca aactaaacg ctttttgga agtgtctgg aaatgccat gttttggat caatttagg agaatattc agacaccac tgccattat taatccctt atggaattt gtatttgac tgctgttaa gctaactga ttatgtagc tttaagtca taggccaga tgacatcat tattttgca aaatgaact gaaaagatt gtttttgca cgaaaattt agccaattc gtatatcat tcttgatga tgtcaaatc gccagatgt tctaaatgc ttttaaatt aagttgtaa aactattgt gaccacgca ggataaaaa tgtggccaa caattcgga ggcattttt cattaaggt tacgacagg ttttactaa aaaagaatc aatcgaatt gttacgaaa atctctaga aactgacaa tctcgcaaa tagggaaaa tctagcaaa tcgaataca cgagttgtg cgatggttg tcccatgtt gttaaatat cataggttc aattttaaa agatagccg cgaagaagc cttgtctac tgatatcat ctggaaatc actaatcga tgaaattat cacaaacga ttacttgca acacccaac aacaatgaa agtgattca catgatcca aagattcgt tacctgctt agaccccga agttcaaag gtgcttcca cagcttggc tgtttttcc caaacatgt caacattcg accggaaat actcatgaa attgtggga caagacgta ttatgaagt gagacaaat aatggtaga gttagaaaa caagtctct catcgtttc cttttacaa caaagatct caaatcgta catatacag tatccatag tttagttcg cacgtattt agttgaaaa agcgaaaat cgacattaa atactacca cgaaaaact gatcttgca atgtgatga aatcatcga caagtcacc gcctgatga ccctattgc taactacat ttttggcta ttacgggta tcatgccaa aaacgcgca actttacca caaattcga aaaatattt cgatttaag attcattga gaacaaaat aaaacgggt cggatcggc tgatctcct gactgattt cgaaatata cagggattt catcacaaa gaaggaacc tcacttgga gcgcaaact gaaagacta cataaagtt tgtgcactc atacagcgc ccatttgca catttctcc cgagacaaa tatcgtgca gtttgcgtt tttcaatga caataatta cgtctacga agatgcaaa acatatcgc cagcaataa tcccgacca actgtactt caaattgga gccgtattc tggtggaca taagaattt tccagtttt aaagacgaa aagtgtggc aactgctac gtgttttgt cgattcgaa ggaggttct cgtgggcac agctagcgg cagcattga agtcaagtc gttccgtca ataccagca caaagtcca ccgctttga aggccactc tgaccaagt cttcagctt gcaaataag tcccgattc aaaaagctt tctctcaat ttccgtcga ccacactgc caaactgtg gcgatttgt cgaagaact cgaccctca atcgcccaa actgaaaca aaccaattg gaaaaactt acattcccc tgatgaaaa tgcaatccc acacaaaac tttcaaaat tcaagacgg cgactacat cacatctgc ggctttccc ttatgatac atgcgatga gataattac ggcttcgaa taaaggaaa aattatgag atggaatgt gagaactgc gtcaattgt gagggaatc atcggcttt aggattgcc aatttcgcg gatttgcta tgttcacga tagtgagca ggtgatttt tgcgtacga tggcaaggt gtattgcta tgccagggg gaatttggt ttataatgg acagtttgg ggttttgga tgatattac cgatttggt tcttattcc gattgtttc ggagtatag ggaagtggc tgtgattag tggaaggaa gcttgagat ctgggaata tgtgaagaa agtcaagag atttagttt tgaagccca atcgaatat tacagctac tgtgatcaa tgacgaata cttggtttt gggcacggc tgagtacat ttatttgta cgattatag gtgtcagaa aattgatca atacacaaa cgatcatgg ggaacccac ctcacttta catccttcg ggaaagcat cgctttcat ttgtctcga taaggaaaa cgtacaacg atgcgaatt atacttcga accctgcag taaaccttt agtcaaaga cctggctct cttcaattg ctactggaa agagtcaca accgattta tttcatcgt taatagtgc aaataaaat cgatatatt ctacggtca ttcaacaat cctggcagg aattgatga aatcaccga taaaattat ttcgtgttc attctccct ctgtggaaa ttacctgat tttggggac ggagtcggg gaagttgta cttttacaa ttacaagaa gaagaaagt cacttgttt gaaaatctg cgatgagga aataaaatt ggttaagtg cttcgaatt cgatgaagt tgttgatta tgacagtgt tagtgcttg cggaatggt tgtggttgc ttctagttc aaaaatcgt gataatagt tgataatag agtgaaatt ggagcttga tattttgga tgaattgac cgacgtgtt gtaccttcg gaaccgctt gattgcgtt tgatgtcaa aggccggtt ttcaatcgc ctgcttaag tgacgatca atgtttagg ctggatttc cacaaagta taaagacgt ggctattgc tagaattca caaagggaa aaatctgct agcgattgc ctgcaagga agggaatgg ttacgtttt gcgagtcct tacattcgt ttctgataa tcctttgac tctgcaatg tttatttga ggcgaagtt gaacacgac tccgacttg catgagctt gaattccaa cggcaaact cgtctcagt tggtggtga taatgggga catagaggt gttttcgat cgaaaacaa ccaacaaga cttgctgct tttgcataa aaagtcagt cgaacagct tgttttcgc tcccaattt cgagtcgat tcttgtgag tttggggga tcagatcgc ttggtggaa cttgaatga ttttggaaa aaaaagtga gaaaatgaa tggaaattc tgagcattc ccaccagtc tttggtgtt caattttga tccatggca agacaagaa acttcatga cactgtgcc ttatttgct cactagtac aaatttaca cggaaagag gttgcagta catttctgc ttcgaaaga ttttcgatc gtttttgat catcgacat cgacggtaa tatcttcaa aatggatat tatcgattc ctgacgatt gtacttagt gatatattg aaaattgct caaaggtga taacgt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
