Status
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Gene information
General information
TC000831 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6419036..6428190
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000831
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00178

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     head irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 42.86% (includes death as larva, prepupa, pupa, adult)

    Adult morphology
    pronotum median surface irregular - Number of animals showing the phenotype: 9

    Comment: ectopic, median, ap-oriented groove

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000831 RA
      MAIFTNVVQ EFREEIEAN LTLDDVIDT LIAQKIIDE 
KLYIEIWGN QHNRVKYLL DIIVKDGKR CSTFIQILG 
TVARMKHIS ESISSSIEK IRKVIVIGG FPEFSLNYV 
VRNSVANTI KDRALGLDP KLGGILVVY GLPGFGKSC 
LVNQVLQTK RFLEVSGNA MFWINLGEY SDTTAIINP 
LWNLYLTAV KLTDYVALS HRPDDIIIL QNELKRLFL 
HENLANSYI ILDDVKSPD VLNAFKLSC KTIVTTQDK 
NVANNSEAF FIKVTTGFT KKESIELLR KSLETDNLA 
NRENLANRI HELCDGCPM LLNIIGSIL KDSREEALS 
TDIIWKSLI DEIITNDYL QHPTTMKVI HMIQRFVTC 
LDPEVQRCF HSLAVFPKH VNIRPEILM KLWDKTYYE 
VRQIMVELE NKSLIVSFY NKDLKSYIY SIHSLVRTY 
LVEKAKIDI KYYHEKLIL QCDEIIDKS PPDDPIANY 
IFGYYGYHA KNAQLYHKF EKYFDLRFI ENKIKRVGS 
ADLLTDFEI YRDFITKKE PHLERKLKD YIKFVHSYS 
AHLHISPET NIVQFAFFN DNNYVYEDA KHIASNNPD 
QLYFKLEPY SGGHKNFPV LKTKSVATA TCFVDSKEV 
LVGTASGSI EVKSFRQYQ HKVHRFEGH SDQVFSLQI 
SPDSKSFLS ISVDHTAKL WRFVEELDP QSPKLKQTN 
WKNLHSPDE NAIPHKTFK IQDGDYITS AAFPYDTCD 
EIITASNKG KIMRWNVRT ASIVRESSA LGLPISRIC 
YVHDSEQVI FAYDGKVYC YARGNLVYN GQFGVLDDI 
TDLVLIPIV SEYREVAVI SGRKLEIWE YVKKVKRFS 
FEAQSNITA TVINDEYLV LGTAEYIYL YDYRCQKID 
QYTNDHGEP TSLYILRES IAFICLDKE NVQRCELYF 
EPCSKPLVK DLALFNCYW KESQPIYFI VNSANKIDI 
FYGHSTILA GIDEITDKI ISCSFSLCG NYLILGTES 
GKLYFYNYK KKKVTCLKI CDEEIKLVK CFEFDEVVD 
YDSVSACGM VVVASSSKI VIIVDNRVK LELDILDEL 
TDVLYLRNR LIAFDVKGR FSIACLSDD QCLGWISTK 
YKDVAIARI HKGKNLLAI ACKEGNGYV LRVLTFVSD 
NPLTLQCLF EAKLNTTPT CMSLNSNGK LVSVGGDNG 
DIEVFSIEN NQQDLLLLH KKSVEQLVF APNFESILV 
SLGDQIAWW NLNDFGKKS EKMNGNSEH SHQSLVFNF 
DPWQDKKLH DTVPYLLTS TNLHGKRLQ YISASKDFR 
SFLIIDIDG NIFKMDIID S
    CDS sequence
    • >TC000831 RA
      atggcaatc ttcacaaat gtggtacaa gagtttcgg 
gaagaaatt gaagctaac ctgaccctc gacgatgta 
attgatacg ttaattgca cagaagatc atcgacgaa 
aaactctac attgaaata tggggcaat caacataac 
cgcgtaaag tacttattg gatatcatc gtcaaagat 
ggcaagcgg tgctccaca ttcattcaa attctggga 
actgtggct cgcatgaag cacatttcc gaatcgatc 
agttccagc attgaaaaa atacgaaaa gttattgtg 
attggcggt tttcccgaa ttttccttg aattatgtt 
gtgagaaat tcagtggct aataccatc aaggatagg 
gcgctaggt ctcgaccct aaacttggc ggtattttg 
gtagtgtat ggtttgccc ggttttggt aaatcatgt 
ttagtaaat caagttttg caaactaaa cgctttttg 
gaagtgtct ggaaatgcc atgttttgg atcaattta 
ggagaatat tcagacacc actgccatt attaatccc 
ttatggaat ttgtatttg actgctgtt aagctaact 
gattatgta gctttaagt cataggcca gatgacatc 
attattttg caaaatgaa ctgaaaaga ttgtttttg 
cacgaaaat ttagccaat tcgtatatc attcttgat 
gatgtcaaa tcgccagat gttctaaat gcttttaaa 
ttaagttgt aaaactatt gtgaccacg caggataaa 
aatgtggcc aacaattcg gaggcattt ttcattaag 
gttacgaca ggttttact aaaaaagaa tcaatcgaa 
ttgttacga aaatctcta gaaactgac aatctcgca 
aatagggaa aatctagca aatcgaata cacgagttg 
tgcgatggt tgtcccatg ttgttaaat atcataggt 
tcaatttta aaagatagc cgcgaagaa gccttgtct 
actgatatc atctggaaa tcactaatc gatgaaatt 
atcacaaac gattacttg caacaccca acaacaatg 
aaagtgatt cacatgatc caaagattc gttacctgc 
ttagacccc gaagttcaa aggtgcttc cacagcttg 
gctgttttt cccaaacat gtcaacatt cgaccggaa 
atactcatg aaattgtgg gacaagacg tattatgaa 
gtgagacaa ataatggta gagttagaa aacaagtct 
ctcatcgtt tccttttac aacaaagat ctcaaatcg 
tacatatac agtatccat agtttagtt cgcacgtat 
ttagttgaa aaagcgaaa atcgacatt aaatactac 
cacgaaaaa ctgatcttg caatgtgat gaaatcatc 
gacaagtca ccgcctgat gaccctatt gctaactac 
atttttggc tattacggg tatcatgcc aaaaacgcg 
caactttac cacaaattc gaaaaatat ttcgattta 
agattcatt gagaacaaa ataaaacgg gtcggatcg 
gctgatctc ctgactgat ttcgaaata tacagggat 
ttcatcaca aagaaggaa cctcacttg gagcgcaaa 
ctgaaagac tacataaag tttgtgcac tcatacagc 
gcccatttg cacatttct cccgagaca aatatcgtg 
cagtttgcg tttttcaat gacaataat tacgtctac 
gaagatgca aaacatatc gccagcaat aatcccgac 
caactgtac ttcaaattg gagccgtat tctggtgga 
cataagaat tttccagtt ttaaagacg aaaagtgtg 
gcaactgct acgtgtttt gtcgattcg aaggaggtt 
ctcgtgggc acagctagc ggcagcatt gaagtcaag 
tcgttccgt caataccag cacaaagtc caccgcttt 
gaaggccac tctgaccaa gtcttcagc ttgcaaata 
agtcccgat tcaaaaagc tttctctca atttccgtc 
gaccacact gccaaactg tggcgattt gtcgaagaa 
ctcgaccct caatcgccc aaactgaaa caaaccaat 
tggaaaaac ttacattcc cctgatgaa aatgcaatc 
ccacacaaa actttcaaa attcaagac ggcgactac 
atcacatct gcggctttc ccttatgat acatgcgat 
gagataatt acggcttcg aataaagga aaaattatg 
agatggaat gtgagaact gcgtcaatt gtgagggaa 
tcatcggct ttaggattg ccaatttcg cggatttgc 
tatgttcac gatagtgag caggtgatt tttgcgtac 
gatggcaag gtgtattgc tatgccagg gggaatttg 
gtttataat ggacagttt ggggttttg gatgatatt 
accgatttg gttcttatt ccgattgtt tcggagtat 
agggaagtg gctgtgatt agtggaagg aagcttgag 
atctgggaa tatgtgaag aaagtcaag agatttagt 
tttgaagcc caatcgaat attacagct actgtgatc 
aatgacgaa tacttggtt ttgggcacg gctgagtac 
atttatttg tacgattat aggtgtcag aaaattgat 
caatacaca aacgatcat ggggaaccc acctcactt 
tacatcctt cgggaaagc atcgctttc atttgtctc 
gataaggaa aacgtacaa cgatgcgaa ttatacttc 
gaaccctgc agtaaacct ttagtcaaa gacctggct 
ctcttcaat tgctactgg aaagagtca caaccgatt 
tatttcatc gttaatagt gcaaataaa atcgatata 
ttctacggt cattcaaca atcctggca ggaattgat 
gaaatcacc gataaaatt atttcgtgt tcattctcc 
ctctgtgga aattacctg attttgggg acggagtcg 
gggaagttg tacttttac aattacaag aagaagaaa 
gtcacttgt ttgaaaatc tgcgatgag gaaataaaa 
ttggttaag tgcttcgaa ttcgatgaa gttgttgat 
tatgacagt gttagtgct tgcggaatg gttgtggtt 
gcttctagt tcaaaaatc gtgataata gttgataat 
agagtgaaa ttggagctt gatattttg gatgaattg 
accgacgtg ttgtacctt cggaaccgc ttgattgcg 
tttgatgtc aaaggccgg ttttcaatc gcctgctta 
agtgacgat caatgttta ggctggatt tccacaaag 
tataaagac gtggctatt gctagaatt cacaaaggg 
aaaaatctg ctagcgatt gcctgcaag gaagggaat 
ggttacgtt ttgcgagtc cttacattc gtttctgat 
aatcctttg actctgcaa tgtttattt gaggcgaag 
ttgaacacg actccgact tgcatgagc ttgaattcc 
aacggcaaa ctcgtctca gttggtggt gataatggg 
gacatagag gtgttttcg atcgaaaac aaccaacaa 
gacttgctg cttttgcat aaaaagtca gtcgaacag 
cttgttttc gctcccaat ttcgagtcg attcttgtg 
agtttgggg gatcagatc gcttggtgg aacttgaat 
gattttgga aaaaaaagt gagaaaatg aatggaaat 
tctgagcat tcccaccag tctttggtg ttcaatttt 
gatccatgg caagacaag aaacttcat gacactgtg 
ccttatttg ctcactagt acaaattta cacggaaag 
aggttgcag tacatttct gcttcgaaa gattttcga 
tcgtttttg atcatcgac atcgacggt aatatcttc 
aaaatggat attatcgat tcctga
    mRNA sequence
    • >TC000831 RA
      ttggcggaa gatttgaca tttgacatt gttttgttg 
ttggtttaa caataaata atccaattg gctagttta 
cctgcaagg tttctattg ttaattaga agtccacct 
atacaaaag aattcaaac gaaataacc aaattcaag 
gccgttgag taattcgga cgtctcata taatttgtt 
tgtgtggtt gtggtcaat gaccgcacg aaagatttg 
acgaataga catgcagaa gattctgag tcgtacagg 
ttttgacag gtatttttg agtgtgctt agagtagtg 
tcgttttgt gtttgctca aaaggcgtg atatcgtca 
cttttgata acaaatcga tcataccaa cgatttttc 
ctttattca ttacggaaa aaatactgt ctttaatgc 
taccccgat ggcaatctt cacaaatgt ggtacaaga 
gtttcggga agaaattga agctaacct gaccctcga 
cgatgtaat tgatacgtt aattgcaca gaagatcat 
cgacgaaaa actctacat tgaaatatg gggcaatca 
acataaccg cgtaaagta cttattgga tatcatcgt 
caaagatgg caagcggtg ctccacatt cattcaaat 
tctgggaac tgtggctcg catgaagca catttccga 
atcgatcag ttccagcat tgaaaaaat acgaaaagt 
tattgtgat tggcggttt tcccgaatt ttccttgaa 
ttatgttgt gagaaattc agtggctaa taccatcaa 
ggatagggc gctaggtct cgaccctaa acttggcgg 
tattttggt agtgtatgg tttgcccgg ttttggtaa 
atcatgttt agtaaatca agttttgca aactaaacg 
ctttttgga agtgtctgg aaatgccat gttttggat 
caatttagg agaatattc agacaccac tgccattat 
taatccctt atggaattt gtatttgac tgctgttaa 
gctaactga ttatgtagc tttaagtca taggccaga 
tgacatcat tattttgca aaatgaact gaaaagatt 
gtttttgca cgaaaattt agccaattc gtatatcat 
tcttgatga tgtcaaatc gccagatgt tctaaatgc 
ttttaaatt aagttgtaa aactattgt gaccacgca 
ggataaaaa tgtggccaa caattcgga ggcattttt 
cattaaggt tacgacagg ttttactaa aaaagaatc 
aatcgaatt gttacgaaa atctctaga aactgacaa 
tctcgcaaa tagggaaaa tctagcaaa tcgaataca 
cgagttgtg cgatggttg tcccatgtt gttaaatat 
cataggttc aattttaaa agatagccg cgaagaagc 
cttgtctac tgatatcat ctggaaatc actaatcga 
tgaaattat cacaaacga ttacttgca acacccaac 
aacaatgaa agtgattca catgatcca aagattcgt 
tacctgctt agaccccga agttcaaag gtgcttcca 
cagcttggc tgtttttcc caaacatgt caacattcg 
accggaaat actcatgaa attgtggga caagacgta 
ttatgaagt gagacaaat aatggtaga gttagaaaa 
caagtctct catcgtttc cttttacaa caaagatct 
caaatcgta catatacag tatccatag tttagttcg 
cacgtattt agttgaaaa agcgaaaat cgacattaa 
atactacca cgaaaaact gatcttgca atgtgatga 
aatcatcga caagtcacc gcctgatga ccctattgc 
taactacat ttttggcta ttacgggta tcatgccaa 
aaacgcgca actttacca caaattcga aaaatattt 
cgatttaag attcattga gaacaaaat aaaacgggt 
cggatcggc tgatctcct gactgattt cgaaatata 
cagggattt catcacaaa gaaggaacc tcacttgga 
gcgcaaact gaaagacta cataaagtt tgtgcactc 
atacagcgc ccatttgca catttctcc cgagacaaa 
tatcgtgca gtttgcgtt tttcaatga caataatta 
cgtctacga agatgcaaa acatatcgc cagcaataa 
tcccgacca actgtactt caaattgga gccgtattc 
tggtggaca taagaattt tccagtttt aaagacgaa 
aagtgtggc aactgctac gtgttttgt cgattcgaa 
ggaggttct cgtgggcac agctagcgg cagcattga 
agtcaagtc gttccgtca ataccagca caaagtcca 
ccgctttga aggccactc tgaccaagt cttcagctt 
gcaaataag tcccgattc aaaaagctt tctctcaat 
ttccgtcga ccacactgc caaactgtg gcgatttgt 
cgaagaact cgaccctca atcgcccaa actgaaaca 
aaccaattg gaaaaactt acattcccc tgatgaaaa 
tgcaatccc acacaaaac tttcaaaat tcaagacgg 
cgactacat cacatctgc ggctttccc ttatgatac 
atgcgatga gataattac ggcttcgaa taaaggaaa 
aattatgag atggaatgt gagaactgc gtcaattgt 
gagggaatc atcggcttt aggattgcc aatttcgcg 
gatttgcta tgttcacga tagtgagca ggtgatttt 
tgcgtacga tggcaaggt gtattgcta tgccagggg 
gaatttggt ttataatgg acagtttgg ggttttgga 
tgatattac cgatttggt tcttattcc gattgtttc 
ggagtatag ggaagtggc tgtgattag tggaaggaa 
gcttgagat ctgggaata tgtgaagaa agtcaagag 
atttagttt tgaagccca atcgaatat tacagctac 
tgtgatcaa tgacgaata cttggtttt gggcacggc 
tgagtacat ttatttgta cgattatag gtgtcagaa 
aattgatca atacacaaa cgatcatgg ggaacccac 
ctcacttta catccttcg ggaaagcat cgctttcat 
ttgtctcga taaggaaaa cgtacaacg atgcgaatt 
atacttcga accctgcag taaaccttt agtcaaaga 
cctggctct cttcaattg ctactggaa agagtcaca 
accgattta tttcatcgt taatagtgc aaataaaat 
cgatatatt ctacggtca ttcaacaat cctggcagg 
aattgatga aatcaccga taaaattat ttcgtgttc 
attctccct ctgtggaaa ttacctgat tttggggac 
ggagtcggg gaagttgta cttttacaa ttacaagaa 
gaagaaagt cacttgttt gaaaatctg cgatgagga 
aataaaatt ggttaagtg cttcgaatt cgatgaagt 
tgttgatta tgacagtgt tagtgcttg cggaatggt 
tgtggttgc ttctagttc aaaaatcgt gataatagt 
tgataatag agtgaaatt ggagcttga tattttgga 
tgaattgac cgacgtgtt gtaccttcg gaaccgctt 
gattgcgtt tgatgtcaa aggccggtt ttcaatcgc 
ctgcttaag tgacgatca atgtttagg ctggatttc 
cacaaagta taaagacgt ggctattgc tagaattca 
caaagggaa aaatctgct agcgattgc ctgcaagga 
agggaatgg ttacgtttt gcgagtcct tacattcgt 
ttctgataa tcctttgac tctgcaatg tttatttga 
ggcgaagtt gaacacgac tccgacttg catgagctt 
gaattccaa cggcaaact cgtctcagt tggtggtga 
taatgggga catagaggt gttttcgat cgaaaacaa 
ccaacaaga cttgctgct tttgcataa aaagtcagt 
cgaacagct tgttttcgc tcccaattt cgagtcgat 
tcttgtgag tttggggga tcagatcgc ttggtggaa 
cttgaatga ttttggaaa aaaaagtga gaaaatgaa 
tggaaattc tgagcattc ccaccagtc tttggtgtt 
caattttga tccatggca agacaagaa acttcatga 
cactgtgcc ttatttgct cactagtac aaatttaca 
cggaaagag gttgcagta catttctgc ttcgaaaga 
ttttcgatc gtttttgat catcgacat cgacggtaa 
tatcttcaa aatggatat tatcgattc ctgacgatt 
gtacttagt gatatattg aaaattgct caaaggtga 
taacgt
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