Status
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Gene information
General information
TC000831 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6419036..6428190
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000831
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00178

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 42.86% (includes death as larva, prepupa, pupa, adult)

    Adult morphology
    pronotum median surface irregular - Number of animals showing the phenotype: 9

    Comment: ectopic, median, ap-oriented groove

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000831 RA
      MAIFTNVVQ EFREEIEAN LTLDDVIDT LIAQKIIDE 
      KLYIEIWGN QHNRVKYLL DIIVKDGKR CSTFIQILG 
      TVARMKHIS ESISSSIEK IRKVIVIGG FPEFSLNYV 
      VRNSVANTI KDRALGLDP KLGGILVVY GLPGFGKSC 
      LVNQVLQTK RFLEVSGNA MFWINLGEY SDTTAIINP 
      LWNLYLTAV KLTDYVALS HRPDDIIIL QNELKRLFL 
      HENLANSYI ILDDVKSPD VLNAFKLSC KTIVTTQDK 
      NVANNSEAF FIKVTTGFT KKESIELLR KSLETDNLA 
      NRENLANRI HELCDGCPM LLNIIGSIL KDSREEALS 
      TDIIWKSLI DEIITNDYL QHPTTMKVI HMIQRFVTC 
      LDPEVQRCF HSLAVFPKH VNIRPEILM KLWDKTYYE 
      VRQIMVELE NKSLIVSFY NKDLKSYIY SIHSLVRTY 
      LVEKAKIDI KYYHEKLIL QCDEIIDKS PPDDPIANY 
      IFGYYGYHA KNAQLYHKF EKYFDLRFI ENKIKRVGS 
      ADLLTDFEI YRDFITKKE PHLERKLKD YIKFVHSYS 
      AHLHISPET NIVQFAFFN DNNYVYEDA KHIASNNPD 
      QLYFKLEPY SGGHKNFPV LKTKSVATA TCFVDSKEV 
      LVGTASGSI EVKSFRQYQ HKVHRFEGH SDQVFSLQI 
      SPDSKSFLS ISVDHTAKL WRFVEELDP QSPKLKQTN 
      WKNLHSPDE NAIPHKTFK IQDGDYITS AAFPYDTCD 
      EIITASNKG KIMRWNVRT ASIVRESSA LGLPISRIC 
      YVHDSEQVI FAYDGKVYC YARGNLVYN GQFGVLDDI 
      TDLVLIPIV SEYREVAVI SGRKLEIWE YVKKVKRFS 
      FEAQSNITA TVINDEYLV LGTAEYIYL YDYRCQKID 
      QYTNDHGEP TSLYILRES IAFICLDKE NVQRCELYF 
      EPCSKPLVK DLALFNCYW KESQPIYFI VNSANKIDI 
      FYGHSTILA GIDEITDKI ISCSFSLCG NYLILGTES 
      GKLYFYNYK KKKVTCLKI CDEEIKLVK CFEFDEVVD 
      YDSVSACGM VVVASSSKI VIIVDNRVK LELDILDEL 
      TDVLYLRNR LIAFDVKGR FSIACLSDD QCLGWISTK 
      YKDVAIARI HKGKNLLAI ACKEGNGYV LRVLTFVSD 
      NPLTLQCLF EAKLNTTPT CMSLNSNGK LVSVGGDNG 
      DIEVFSIEN NQQDLLLLH KKSVEQLVF APNFESILV 
      SLGDQIAWW NLNDFGKKS EKMNGNSEH SHQSLVFNF 
      DPWQDKKLH DTVPYLLTS TNLHGKRLQ YISASKDFR 
      SFLIIDIDG NIFKMDIID S
    CDS sequence
    • >TC000831 RA
      atggcaatc ttcacaaat gtggtacaa gagtttcgg 
      gaagaaatt gaagctaac ctgaccctc gacgatgta 
      attgatacg ttaattgca cagaagatc atcgacgaa 
      aaactctac attgaaata tggggcaat caacataac 
      cgcgtaaag tacttattg gatatcatc gtcaaagat 
      ggcaagcgg tgctccaca ttcattcaa attctggga 
      actgtggct cgcatgaag cacatttcc gaatcgatc 
      agttccagc attgaaaaa atacgaaaa gttattgtg 
      attggcggt tttcccgaa ttttccttg aattatgtt 
      gtgagaaat tcagtggct aataccatc aaggatagg 
      gcgctaggt ctcgaccct aaacttggc ggtattttg 
      gtagtgtat ggtttgccc ggttttggt aaatcatgt 
      ttagtaaat caagttttg caaactaaa cgctttttg 
      gaagtgtct ggaaatgcc atgttttgg atcaattta 
      ggagaatat tcagacacc actgccatt attaatccc 
      ttatggaat ttgtatttg actgctgtt aagctaact 
      gattatgta gctttaagt cataggcca gatgacatc 
      attattttg caaaatgaa ctgaaaaga ttgtttttg 
      cacgaaaat ttagccaat tcgtatatc attcttgat 
      gatgtcaaa tcgccagat gttctaaat gcttttaaa 
      ttaagttgt aaaactatt gtgaccacg caggataaa 
      aatgtggcc aacaattcg gaggcattt ttcattaag 
      gttacgaca ggttttact aaaaaagaa tcaatcgaa 
      ttgttacga aaatctcta gaaactgac aatctcgca 
      aatagggaa aatctagca aatcgaata cacgagttg 
      tgcgatggt tgtcccatg ttgttaaat atcataggt 
      tcaatttta aaagatagc cgcgaagaa gccttgtct 
      actgatatc atctggaaa tcactaatc gatgaaatt 
      atcacaaac gattacttg caacaccca acaacaatg 
      aaagtgatt cacatgatc caaagattc gttacctgc 
      ttagacccc gaagttcaa aggtgcttc cacagcttg 
      gctgttttt cccaaacat gtcaacatt cgaccggaa 
      atactcatg aaattgtgg gacaagacg tattatgaa 
      gtgagacaa ataatggta gagttagaa aacaagtct 
      ctcatcgtt tccttttac aacaaagat ctcaaatcg 
      tacatatac agtatccat agtttagtt cgcacgtat 
      ttagttgaa aaagcgaaa atcgacatt aaatactac 
      cacgaaaaa ctgatcttg caatgtgat gaaatcatc 
      gacaagtca ccgcctgat gaccctatt gctaactac 
      atttttggc tattacggg tatcatgcc aaaaacgcg 
      caactttac cacaaattc gaaaaatat ttcgattta 
      agattcatt gagaacaaa ataaaacgg gtcggatcg 
      gctgatctc ctgactgat ttcgaaata tacagggat 
      ttcatcaca aagaaggaa cctcacttg gagcgcaaa 
      ctgaaagac tacataaag tttgtgcac tcatacagc 
      gcccatttg cacatttct cccgagaca aatatcgtg 
      cagtttgcg tttttcaat gacaataat tacgtctac 
      gaagatgca aaacatatc gccagcaat aatcccgac 
      caactgtac ttcaaattg gagccgtat tctggtgga 
      cataagaat tttccagtt ttaaagacg aaaagtgtg 
      gcaactgct acgtgtttt gtcgattcg aaggaggtt 
      ctcgtgggc acagctagc ggcagcatt gaagtcaag 
      tcgttccgt caataccag cacaaagtc caccgcttt 
      gaaggccac tctgaccaa gtcttcagc ttgcaaata 
      agtcccgat tcaaaaagc tttctctca atttccgtc 
      gaccacact gccaaactg tggcgattt gtcgaagaa 
      ctcgaccct caatcgccc aaactgaaa caaaccaat 
      tggaaaaac ttacattcc cctgatgaa aatgcaatc 
      ccacacaaa actttcaaa attcaagac ggcgactac 
      atcacatct gcggctttc ccttatgat acatgcgat 
      gagataatt acggcttcg aataaagga aaaattatg 
      agatggaat gtgagaact gcgtcaatt gtgagggaa 
      tcatcggct ttaggattg ccaatttcg cggatttgc 
      tatgttcac gatagtgag caggtgatt tttgcgtac 
      gatggcaag gtgtattgc tatgccagg gggaatttg 
      gtttataat ggacagttt ggggttttg gatgatatt 
      accgatttg gttcttatt ccgattgtt tcggagtat 
      agggaagtg gctgtgatt agtggaagg aagcttgag 
      atctgggaa tatgtgaag aaagtcaag agatttagt 
      tttgaagcc caatcgaat attacagct actgtgatc 
      aatgacgaa tacttggtt ttgggcacg gctgagtac 
      atttatttg tacgattat aggtgtcag aaaattgat 
      caatacaca aacgatcat ggggaaccc acctcactt 
      tacatcctt cgggaaagc atcgctttc atttgtctc 
      gataaggaa aacgtacaa cgatgcgaa ttatacttc 
      gaaccctgc agtaaacct ttagtcaaa gacctggct 
      ctcttcaat tgctactgg aaagagtca caaccgatt 
      tatttcatc gttaatagt gcaaataaa atcgatata 
      ttctacggt cattcaaca atcctggca ggaattgat 
      gaaatcacc gataaaatt atttcgtgt tcattctcc 
      ctctgtgga aattacctg attttgggg acggagtcg 
      gggaagttg tacttttac aattacaag aagaagaaa 
      gtcacttgt ttgaaaatc tgcgatgag gaaataaaa 
      ttggttaag tgcttcgaa ttcgatgaa gttgttgat 
      tatgacagt gttagtgct tgcggaatg gttgtggtt 
      gcttctagt tcaaaaatc gtgataata gttgataat 
      agagtgaaa ttggagctt gatattttg gatgaattg 
      accgacgtg ttgtacctt cggaaccgc ttgattgcg 
      tttgatgtc aaaggccgg ttttcaatc gcctgctta 
      agtgacgat caatgttta ggctggatt tccacaaag 
      tataaagac gtggctatt gctagaatt cacaaaggg 
      aaaaatctg ctagcgatt gcctgcaag gaagggaat 
      ggttacgtt ttgcgagtc cttacattc gtttctgat 
      aatcctttg actctgcaa tgtttattt gaggcgaag 
      ttgaacacg actccgact tgcatgagc ttgaattcc 
      aacggcaaa ctcgtctca gttggtggt gataatggg 
      gacatagag gtgttttcg atcgaaaac aaccaacaa 
      gacttgctg cttttgcat aaaaagtca gtcgaacag 
      cttgttttc gctcccaat ttcgagtcg attcttgtg 
      agtttgggg gatcagatc gcttggtgg aacttgaat 
      gattttgga aaaaaaagt gagaaaatg aatggaaat 
      tctgagcat tcccaccag tctttggtg ttcaatttt 
      gatccatgg caagacaag aaacttcat gacactgtg 
      ccttatttg ctcactagt acaaattta cacggaaag 
      aggttgcag tacatttct gcttcgaaa gattttcga 
      tcgtttttg atcatcgac atcgacggt aatatcttc 
      aaaatggat attatcgat tcctga
    mRNA sequence
    • >TC000831 RA
      ttggcggaa gatttgaca tttgacatt gttttgttg 
      ttggtttaa caataaata atccaattg gctagttta 
      cctgcaagg tttctattg ttaattaga agtccacct 
      atacaaaag aattcaaac gaaataacc aaattcaag 
      gccgttgag taattcgga cgtctcata taatttgtt 
      tgtgtggtt gtggtcaat gaccgcacg aaagatttg 
      acgaataga catgcagaa gattctgag tcgtacagg 
      ttttgacag gtatttttg agtgtgctt agagtagtg 
      tcgttttgt gtttgctca aaaggcgtg atatcgtca 
      cttttgata acaaatcga tcataccaa cgatttttc 
      ctttattca ttacggaaa aaatactgt ctttaatgc 
      taccccgat ggcaatctt cacaaatgt ggtacaaga 
      gtttcggga agaaattga agctaacct gaccctcga 
      cgatgtaat tgatacgtt aattgcaca gaagatcat 
      cgacgaaaa actctacat tgaaatatg gggcaatca 
      acataaccg cgtaaagta cttattgga tatcatcgt 
      caaagatgg caagcggtg ctccacatt cattcaaat 
      tctgggaac tgtggctcg catgaagca catttccga 
      atcgatcag ttccagcat tgaaaaaat acgaaaagt 
      tattgtgat tggcggttt tcccgaatt ttccttgaa 
      ttatgttgt gagaaattc agtggctaa taccatcaa 
      ggatagggc gctaggtct cgaccctaa acttggcgg 
      tattttggt agtgtatgg tttgcccgg ttttggtaa 
      atcatgttt agtaaatca agttttgca aactaaacg 
      ctttttgga agtgtctgg aaatgccat gttttggat 
      caatttagg agaatattc agacaccac tgccattat 
      taatccctt atggaattt gtatttgac tgctgttaa 
      gctaactga ttatgtagc tttaagtca taggccaga 
      tgacatcat tattttgca aaatgaact gaaaagatt 
      gtttttgca cgaaaattt agccaattc gtatatcat 
      tcttgatga tgtcaaatc gccagatgt tctaaatgc 
      ttttaaatt aagttgtaa aactattgt gaccacgca 
      ggataaaaa tgtggccaa caattcgga ggcattttt 
      cattaaggt tacgacagg ttttactaa aaaagaatc 
      aatcgaatt gttacgaaa atctctaga aactgacaa 
      tctcgcaaa tagggaaaa tctagcaaa tcgaataca 
      cgagttgtg cgatggttg tcccatgtt gttaaatat 
      cataggttc aattttaaa agatagccg cgaagaagc 
      cttgtctac tgatatcat ctggaaatc actaatcga 
      tgaaattat cacaaacga ttacttgca acacccaac 
      aacaatgaa agtgattca catgatcca aagattcgt 
      tacctgctt agaccccga agttcaaag gtgcttcca 
      cagcttggc tgtttttcc caaacatgt caacattcg 
      accggaaat actcatgaa attgtggga caagacgta 
      ttatgaagt gagacaaat aatggtaga gttagaaaa 
      caagtctct catcgtttc cttttacaa caaagatct 
      caaatcgta catatacag tatccatag tttagttcg 
      cacgtattt agttgaaaa agcgaaaat cgacattaa 
      atactacca cgaaaaact gatcttgca atgtgatga 
      aatcatcga caagtcacc gcctgatga ccctattgc 
      taactacat ttttggcta ttacgggta tcatgccaa 
      aaacgcgca actttacca caaattcga aaaatattt 
      cgatttaag attcattga gaacaaaat aaaacgggt 
      cggatcggc tgatctcct gactgattt cgaaatata 
      cagggattt catcacaaa gaaggaacc tcacttgga 
      gcgcaaact gaaagacta cataaagtt tgtgcactc 
      atacagcgc ccatttgca catttctcc cgagacaaa 
      tatcgtgca gtttgcgtt tttcaatga caataatta 
      cgtctacga agatgcaaa acatatcgc cagcaataa 
      tcccgacca actgtactt caaattgga gccgtattc 
      tggtggaca taagaattt tccagtttt aaagacgaa 
      aagtgtggc aactgctac gtgttttgt cgattcgaa 
      ggaggttct cgtgggcac agctagcgg cagcattga 
      agtcaagtc gttccgtca ataccagca caaagtcca 
      ccgctttga aggccactc tgaccaagt cttcagctt 
      gcaaataag tcccgattc aaaaagctt tctctcaat 
      ttccgtcga ccacactgc caaactgtg gcgatttgt 
      cgaagaact cgaccctca atcgcccaa actgaaaca 
      aaccaattg gaaaaactt acattcccc tgatgaaaa 
      tgcaatccc acacaaaac tttcaaaat tcaagacgg 
      cgactacat cacatctgc ggctttccc ttatgatac 
      atgcgatga gataattac ggcttcgaa taaaggaaa 
      aattatgag atggaatgt gagaactgc gtcaattgt 
      gagggaatc atcggcttt aggattgcc aatttcgcg 
      gatttgcta tgttcacga tagtgagca ggtgatttt 
      tgcgtacga tggcaaggt gtattgcta tgccagggg 
      gaatttggt ttataatgg acagtttgg ggttttgga 
      tgatattac cgatttggt tcttattcc gattgtttc 
      ggagtatag ggaagtggc tgtgattag tggaaggaa 
      gcttgagat ctgggaata tgtgaagaa agtcaagag 
      atttagttt tgaagccca atcgaatat tacagctac 
      tgtgatcaa tgacgaata cttggtttt gggcacggc 
      tgagtacat ttatttgta cgattatag gtgtcagaa 
      aattgatca atacacaaa cgatcatgg ggaacccac 
      ctcacttta catccttcg ggaaagcat cgctttcat 
      ttgtctcga taaggaaaa cgtacaacg atgcgaatt 
      atacttcga accctgcag taaaccttt agtcaaaga 
      cctggctct cttcaattg ctactggaa agagtcaca 
      accgattta tttcatcgt taatagtgc aaataaaat 
      cgatatatt ctacggtca ttcaacaat cctggcagg 
      aattgatga aatcaccga taaaattat ttcgtgttc 
      attctccct ctgtggaaa ttacctgat tttggggac 
      ggagtcggg gaagttgta cttttacaa ttacaagaa 
      gaagaaagt cacttgttt gaaaatctg cgatgagga 
      aataaaatt ggttaagtg cttcgaatt cgatgaagt 
      tgttgatta tgacagtgt tagtgcttg cggaatggt 
      tgtggttgc ttctagttc aaaaatcgt gataatagt 
      tgataatag agtgaaatt ggagcttga tattttgga 
      tgaattgac cgacgtgtt gtaccttcg gaaccgctt 
      gattgcgtt tgatgtcaa aggccggtt ttcaatcgc 
      ctgcttaag tgacgatca atgtttagg ctggatttc 
      cacaaagta taaagacgt ggctattgc tagaattca 
      caaagggaa aaatctgct agcgattgc ctgcaagga 
      agggaatgg ttacgtttt gcgagtcct tacattcgt 
      ttctgataa tcctttgac tctgcaatg tttatttga 
      ggcgaagtt gaacacgac tccgacttg catgagctt 
      gaattccaa cggcaaact cgtctcagt tggtggtga 
      taatgggga catagaggt gttttcgat cgaaaacaa 
      ccaacaaga cttgctgct tttgcataa aaagtcagt 
      cgaacagct tgttttcgc tcccaattt cgagtcgat 
      tcttgtgag tttggggga tcagatcgc ttggtggaa 
      cttgaatga ttttggaaa aaaaagtga gaaaatgaa 
      tggaaattc tgagcattc ccaccagtc tttggtgtt 
      caattttga tccatggca agacaagaa acttcatga 
      cactgtgcc ttatttgct cactagtac aaatttaca 
      cggaaagag gttgcagta catttctgc ttcgaaaga 
      ttttcgatc gtttttgat catcgacat cgacggtaa 
      tatcttcaa aatggatat tatcgattc ctgacgatt 
      gtacttagt gatatattg aaaattgct caaaggtga 
      taacgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY