Status
Please wait ...
Gene information
General information
TC000821 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 734483..741720
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000821
    iBeetle screen iB_00177
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000821 RA
      MKKITLFMI IICFERNLS YKVMYNGNN NVTDLVEYV 
      LLNEDNPCA RKCVKDSVP MTCRYTFLL EWYHTLSKA 
      CYDCPYNTQ DCYREDCIP GDGNKRSII VVNRKMPGP 
      SVEVCLGDE VIIDVVNHL SSDSTTIHW HGHHQKNSP 
      YMDGVPFVT QCPIHPGMT FRYHFNVHN SGTHFWHSH 
      SGFQRSDGT FGPFIVRVP EEDNPHAKL YDYDLSSHV 
      ITILDWTKE DGTDKFMSH IHNDGDNKP DTILVNGFG 
      RFKHFVGAD NSTVFVPTA RFTVEQGYR YRFRVINAG 
      FLNCPIEVS IDNHTLSVI STDGSDFNA TEVDSLVTY 
      AGERFDFIV TADQPQDVY WMHFRGLMD CDERFTRAY 
      QVAVLEYKG TQTNYPSYE PTYDNSRRE GKQLNPLNK 
      GTEADSSFV TLPQLHSLD EWDDTLKEK ADFQYYVSY 
      DFYKMNHPV YHKDPHYGF HNVTNTTLQ NLTPQLNYI 
      SMKLQSFPL LSQRHQIDA KMFCNESSV SNCENEYCE 
      CTHVVNIPL GTVVEMVLI DKGYAYDAN HPFHLHGHS 
      FRVVAMERV GSHVNVSEI LKMDQNGQI KRNLVDAPL 
      KDTVTVPDG GFTIIRFKA TNPGYWLFH CHIEFHVEV 
      GMALVFKIG EDYEMPPVP KDFPQCGDY VPSGNSTVD 
      CDDVGTFGA ILKKLLPKV YEDYCPTSN SGSVRMSHL 
      GTLVPLILF MLWG
    CDS sequence
    • >TC000821 RA
      atgaaaaaa attacactc tttatgatc attatttgc 
      tttgaacga aatctctcc tacaaagtg atgtataat 
      ggcaacaac aacgtgact gacctggtc gagtacgtt 
      ttactaaat gaagataac ccttgcgca agaaaatgt 
      gtaaaagat tcggtacca atgacatgc aggtacact 
      ttcctactg gaatggtac cacacattg agtaaagct 
      tgctacgat tgtccttac aacacccaa gactgctat 
      cgggaggac tgtataccg ggtgatggc aataagcgg 
      tccattatt gtagtcaac cgcaaaatg ccaggaccc 
      agcgttgag gtttgtttg ggtgacgaa gtgataata 
      gacgttgtg aaccactta tctagtgat agtaccact 
      atccattgg catggccac caccaaaaa aacagccct 
      tatatggac ggggtacca tttgtaacc cagtgtccc 
      atccatcct ggtatgacc ttccggtac catttcaat 
      gtccataat tccgggacg cacttctgg cactctcat 
      tcaggtttc caaagatcc gatggtact tttggccca 
      tttatcgtg cgagtgcct gaagaagac aacccacat 
      gcgaaactc tacgactat gacttgtca agtcacgtt 
      atcacgatt ctggactgg accaaggag gatggtact 
      gataagttc atgtcccat attcacaac gacggtgat 
      aataaaccg gacaccatt cttgttaat gggtttgga 
      aggtttaaa catttcgtt ggtgcagat aacagtacc 
      gtttttgta ccaacggcc cgatttaca gttgaacag 
      ggttacagg tatcgattt cgcgtgatc aacgccggg 
      ttcttgaac tgtcccatt gaagtgtcc attgataac 
      cacactctt agcgttatc agtaccgat ggtagcgac 
      tttaacgcg actgaagtc gatagtttg gtaacgtac 
      gccggcgaa aggtttgat tttattgtc accgctgac 
      cagcctcaa gacgtgtat tggatgcac tttagaggt 
      ttgatggac tgtgatgaa cgtttcact cgagcttac 
      caagtggca gtactcgaa tataaaggt acacaaacc 
      aactatcca tcatatgaa cccacctat gacaattca 
      agaagagaa ggcaaacaa ttgaatccc ttaaataaa 
      ggtacagaa gccgacagt tcgtttgtt acgctacct 
      cagctccac tcactggac gagtgggac gacaccctc 
      aaagaaaaa gccgatttt cagtattac gtttcgtac 
      gatttctac aaaatgaat caccctgta tatcacaaa 
      gacccacat tatggtttc cataatgtc acaaatacg 
      actttgcaa aatttgaca ccgcaatta aactacatt 
      tcgatgaaa ctccaatcg ttccccttg ttgtcacaa 
      aggcaccaa atcgatgct aaaatgttc tgtaacgaa 
      tcgtcggtc agcaactgc gagaatgaa tattgcgaa 
      tgcacccac gtagtcaat attccttta ggtacagtt 
      gttgagatg gtgcttatt gacaaaggg tacgcttac 
      gacgctaat cacccgttc cacctccac ggtcattcc 
      ttccgtgtg gttgcaatg gaacgtgta gggtcacac 
      gttaacgtt tcggaaatt ttgaaaatg gaccagaat 
      ggacaaatt aagagaaac ctagtagat gcccctctc 
      aaagacact gtgactgtc ccagacggt ggttttaca 
      attatcaga ttcaaagca acaaatccg ggttattgg 
      ttgttccac tgtcatatt gagtttcac gtcgaagtt 
      ggaatggct cttgttttc aaaatcggg gaagattac 
      gaaatgccg ccggttccg aaagatttt ccccaatgt 
      ggtgattat gtcccgagt ggaaatagt acagttgac 
      tgtgatgac gttggcact tttggtgca atcttgaag 
      aaattatta ccgaaagta tacgaggat tattgtccg 
      acttcgaat agtggaagt gtgaggatg tctcacttg 
      ggtacttta gtgccattg atactgttt atgttgtgg 
      ggttga
    mRNA sequence
    • >TC000821 RA
      ggcaatttt cttaaacgg atggtagtc acttttcga 
      attttgatc atattgcaa tcagacata gcactcggg 
      ccgaagtga ttttgtgat tgttttgtg gtcagattc 
      ccaacaaaa tattttttg ttcgataag taaatgcac 
      agtgaataa tgaaaaaaa ttacactct ttatgatca 
      ttatttgct ttgaacgaa atctctcct acaaagtga 
      tgtataatg gcaacaaca acgtgactg acctggtcg 
      agtacgttt tactaaatg aagataacc cttgcgcaa 
      gaaaatgtg taaaagatt cggtaccaa tgacatgca 
      ggtacactt tcctactgg aatggtacc acacattga 
      gtaaagctt gctacgatt gtccttaca acacccaag 
      actgctatc gggaggact gtataccgg gtgatggca 
      ataagcggt ccattattg tagtcaacc gcaaaatgc 
      caggaccca gcgttgagg tttgtttgg gtgacgaag 
      tgataatag acgttgtga accacttat ctagtgata 
      gtaccacta tccattggc atggccacc accaaaaaa 
      acagccctt atatggacg gggtaccat ttgtaaccc 
      agtgtccca tccatcctg gtatgacct tccggtacc 
      atttcaatg tccataatt ccgggacgc acttctggc 
      actctcatt caggtttcc aaagatccg atggtactt 
      ttggcccat ttatcgtgc gagtgcctg aagaagaca 
      acccacatg cgaaactct acgactatg acttgtcaa 
      gtcacgtta tcacgattc tggactgga ccaaggagg 
      atggtactg ataagttca tgtcccata ttcacaacg 
      acggtgata ataaaccgg acaccattc ttgttaatg 
      ggtttggaa ggtttaaac atttcgttg gtgcagata 
      acagtaccg tttttgtac caacggccc gatttacag 
      ttgaacagg gttacaggt atcgatttc gcgtgatca 
      acgccgggt tcttgaact gtcccattg aagtgtcca 
      ttgataacc acactctta gcgttatca gtaccgatg 
      gtagcgact ttaacgcga ctgaagtcg atagtttgg 
      taacgtacg ccggcgaaa ggtttgatt ttattgtca 
      ccgctgacc agcctcaag acgtgtatt ggatgcact 
      ttagaggtt tgatggact gtgatgaac gtttcactc 
      gagcttacc aagtggcag tactcgaat ataaaggta 
      cacaaacca actatccat catatgaac ccacctatg 
      acaattcaa gaagagaag gcaaacaat tgaatccct 
      taaataaag gtacagaag ccgacagtt cgtttgtta 
      cgctacctc agctccact cactggacg agtgggacg 
      acaccctca aagaaaaag ccgattttc agtattacg 
      tttcgtacg atttctaca aaatgaatc accctgtat 
      atcacaaag acccacatt atggtttcc ataatgtca 
      caaatacga ctttgcaaa atttgacac cgcaattaa 
      actacattt cgatgaaac tccaatcgt tccccttgt 
      tgtcacaaa ggcaccaaa tcgatgcta aaatgttct 
      gtaacgaat cgtcggtca gcaactgcg agaatgaat 
      attgcgaat gcacccacg tagtcaata ttcctttag 
      gtacagttg ttgagatgg tgcttattg acaaagggt 
      acgcttacg acgctaatc acccgttcc acctccacg 
      gtcattcct tccgtgtgg ttgcaatgg aacgtgtag 
      ggtcacacg ttaacgttt cggaaattt tgaaaatgg 
      accagaatg gacaaatta agagaaacc tagtagatg 
      cccctctca aagacactg tgactgtcc cagacggtg 
      gttttacaa ttatcagat tcaaagcaa caaatccgg 
      gttattggt tgttccact gtcatattg agtttcacg 
      tcgaagttg gaatggctc ttgttttca aaatcgggg 
      aagattacg aaatgccgc cggttccga aagattttc 
      cccaatgtg gtgattatg tcccgagtg gaaatagta 
      cagttgact gtgatgacg ttggcactt ttggtgcaa 
      tcttgaaga aattattac cgaaagtat acgaggatt 
      attgtccga cttcgaata gtggaagtg tgaggatgt 
      ctcacttgg gtactttag tgccattga tactgttta 
      tgttgtggg gttgatttg tattagtga aatttcgta 
      accaatgtg atacttttt agaatgtac ctatttgtt 
      aaattatta aatatttta gatacaaat tgcgtttta 
      tttcaaaat aaatcacta atcatcaaa gaataaatt 
      aataacaaa tac