Status
Please wait ...
Gene information
General information
TC032056
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000815,TC000814
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 686513..689909
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032056
    iBeetle screen iB_00176
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032056 RA
      MDRSFLLIQ ERTHHDYIE KAQTADFET AIHYSRYGK 
      FQALLLAVC GTIYATCAI SSTTLSFVL PSAKCDFNL 
      SSVDKGKLS AMPLIGMIF GCSLWGSIA DSQGRKVAI 
      MLSLLVDFL AALISSFAQ TFSFFLICR FFNGFGIIG 
      ATSIIFSYL GEFASKKHR DVMLGRLEV FWNVGVILL 
      PGVAWVLLN RTTLDSFID HGNFSPWRI FVLVCSIPS 
      LISLVMLYF LPETPKFLI SKGQYEKAK LVFQKVFTF 
      NTGFPSYAY PVMTLEGEY ENNNNSEGL GTKVPLREK 
      ICVKITSIV SETQVLFTQ PYLKYLSVT CFADFGLMA 
      SYYTLIMWF PEIFDRFNQ FEVTHPNST ASVCSVSNG 
      RADALEVFF PEPCNPSID NRVFLDTII IGLSCVPTS 
      VSLSFFMKK FGKKAVLII GLVMSGLST LSLNWVQSS 
      TETLVLSCV FEALTSILE AVIFCFIVD LFPTNLRAI 
      ALAVTASSG RIGAIFGNV VFGLLIDWQ CFVPIYLFG 
      SLLIASGTL CLVLPQTDK YMVIQ
    • >TC032056 RB
      MDRSFLLIQ ERTHHDYIE KAQTADFET AIHYSRYGK 
      FQALLLAVC GTIYATCAI SSTTLSFVL PSAKCDFNL 
      SSVDKGKLS AMPLIGMIF GCSLWGSIA DSQGRKVAI 
      MLSLLVDFL AALISSFAQ TFSFFLICR FFNGFGIIG 
      ATSIIFSYL GEFASKKHR DVMLGRLEV FWNVGVILL 
      PGVAWVLLN RTTLDSFID HGNFSPWRI FVLVCSIPS 
      LISLVMLYF LPETPKFLI SKGQYEKAK LVFQKVFTF 
      NTGFPSYAY PVMTLEGEY ENNNNSEGL GTKVPLREK 
      ICVKITSIV SETQVLFTQ PYLKYLSVT CFADFGLMA 
      SYYTLIMWF PEIFDRFNQ FEVTHPNST ASVCSVSNG 
      RADALEVFF PEPCNPSID NRVFLDTII IGLSCVPTS 
      VSLSFFMKK FGKKAVLII GLVMSGLST LSLNWVQSS 
      TETLVLSCV FEALTSILE AVIFCFIVD LFPTNLRAI 
      ALAVTASSG RIGAIFGNV VFGLLIDWQ CFVPIYLFG 
      SLLIGKQYY F
    CDS sequence
    • >TC032056 RA
      atggaccgc tctttctta ctaatccaa gaaagaact 
      caccacgat tacatcgaa aaagcccaa accgctgac 
      tttgaaacc gccatccat tactcaagg tatggaaaa 
      ttccaggcc ttgctcctt gctgtttgt gggacaatt 
      tacgcaact tgcgccatc agctcaacg actttatcg 
      ttcgtcttg ccttcggcc aaatgtgac tttaattta 
      tcatcagtt gataagggg aaattatca gcgatgcct 
      ctaatcgga atgattttc gggtgcagt ctttggggc 
      tcaattgct gattctcag ggacgtaaa gtcgcaata 
      atgctttcg ctcctcgtt gattttctc gcagctttg 
      atttcttcc ttcgctcaa actttcagc ttcttttta 
      atttgtcgg ttcttcaac ggattcggt attataggg 
      gccactagt attattttc tcttatttg ggcgaattc 
      gcgtcaaag aaacaccga gatgtgatg ttgggtcgt 
      ttggaagtg ttctggaac gttggagtg atcctacta 
      ccaggtgtg gcctgggtt ctactgaac cgaaccact 
      ttggactca ttcatcgac catggaaat ttcagccct 
      tggcgaatt tttgttctg gtctgcagt atcccaagc 
      ttgatcagt ctagtcatg ttgtatttc ctaccagag 
      acaccaaaa tttctcatt tcgaaaggg caatacgaa 
      aaggctaaa ctcgttttc cagaaggtt ttcaccttt 
      aacactgga tttccctcg tatgcttat ccagtcatg 
      actctagaa ggagagtac gaaaataat aataattct 
      gaaggactg ggcactaaa gtgccactt agggaaaaa 
      atctgtgtc aagattacg tccattgtg agtgaaacg 
      caagtgttg ttcacgcaa ccgtatctg aaatacctc 
      tcagtgact tgttttgcg gattttggc ttaatggca 
      agttactac actttgatt atgtggttt cccgaaatt 
      ttcgaccgt ttcaaccag tttgaagtg actcatccc 
      aacagtact gcaagtgtt tgttccgtg tccaatgga 
      agagcggac gctcttgaa gtatttttc cctgaacct 
      tgcaaccct tcaatagat aatcgtgtt tttctggac 
      acgattatt ataggatta agctgtgtt cctacgtca 
      gtatcgctc agcttcttt atgaagaag ttcggcaag 
      aaagcggta ttaattatt ggattggtg atgtcaggg 
      ttatcaacc ctctcgctg aactgggta caaagttca 
      actgaaact ctcgtgctt tcttgtgtt tttgaagca 
      ctgaccagt attcttgaa gctgtgatt ttttgcttt 
      attgtcgat ttgtttccg acaaatctt agggcaatt 
      gctttagct gtgactgca tcctcaggg agaattggc 
      gcaattttt ggaaatgta gtttttggc ttgttgatc 
      gattggcaa tgttttgta ccgatttat ctgtttggc 
      tcgctcctc atagccagc ggaacgctt tgcttggtc 
      ttacctcaa acggacaaa tacatggta atacaataa
    • >TC032056 RB
      atggaccgc tctttctta ctaatccaa gaaagaact 
      caccacgat tacatcgaa aaagcccaa accgctgac 
      tttgaaacc gccatccat tactcaagg tatggaaaa 
      ttccaggcc ttgctcctt gctgtttgt gggacaatt 
      tacgcaact tgcgccatc agctcaacg actttatcg 
      ttcgtcttg ccttcggcc aaatgtgac tttaattta 
      tcatcagtt gataagggg aaattatca gcgatgcct 
      ctaatcgga atgattttc gggtgcagt ctttggggc 
      tcaattgct gattctcag ggacgtaaa gtcgcaata 
      atgctttcg ctcctcgtt gattttctc gcagctttg 
      atttcttcc ttcgctcaa actttcagc ttcttttta 
      atttgtcgg ttcttcaac ggattcggt attataggg 
      gccactagt attattttc tcttatttg ggcgaattc 
      gcgtcaaag aaacaccga gatgtgatg ttgggtcgt 
      ttggaagtg ttctggaac gttggagtg atcctacta 
      ccaggtgtg gcctgggtt ctactgaac cgaaccact 
      ttggactca ttcatcgac catggaaat ttcagccct 
      tggcgaatt tttgttctg gtctgcagt atcccaagc 
      ttgatcagt ctagtcatg ttgtatttc ctaccagag 
      acaccaaaa tttctcatt tcgaaaggg caatacgaa 
      aaggctaaa ctcgttttc cagaaggtt ttcaccttt 
      aacactgga tttccctcg tatgcttat ccagtcatg 
      actctagaa ggagagtac gaaaataat aataattct 
      gaaggactg ggcactaaa gtgccactt agggaaaaa 
      atctgtgtc aagattacg tccattgtg agtgaaacg 
      caagtgttg ttcacgcaa ccgtatctg aaatacctc 
      tcagtgact tgttttgcg gattttggc ttaatggca 
      agttactac actttgatt atgtggttt cccgaaatt 
      ttcgaccgt ttcaaccag tttgaagtg actcatccc 
      aacagtact gcaagtgtt tgttccgtg tccaatgga 
      agagcggac gctcttgaa gtatttttc cctgaacct 
      tgcaaccct tcaatagat aatcgtgtt tttctggac 
      acgattatt ataggatta agctgtgtt cctacgtca 
      gtatcgctc agcttcttt atgaagaag ttcggcaag 
      aaagcggta ttaattatt ggattggtg atgtcaggg 
      ttatcaacc ctctcgctg aactgggta caaagttca 
      actgaaact ctcgtgctt tcttgtgtt tttgaagca 
      ctgaccagt attcttgaa gctgtgatt ttttgcttt 
      attgtcgat ttgtttccg acaaatctt agggcaatt 
      gctttagct gtgactgca tcctcaggg agaattggc 
      gcaattttt ggaaatgta gtttttggc ttgttgatc 
      gattggcaa tgttttgta ccgatttat ctgtttggc 
      tcgctcctc ataggtaaa caatactat ttttaa
    mRNA sequence
    • >TC032056 RA
      tgtctttat cacgaagcg aaatggacc gctctttct 
      tactaatcc aagaaagaa ctcaccacg attacatcg 
      aaaaagccc aaaccgctg actttgaaa ccgccatcc 
      attactcaa ggtatggaa aattccagg ccttgctcc 
      ttgctgttt gtgggacaa tttacgcaa cttgcgcca 
      tcagctcaa cgactttat cgttcgtct tgccttcgg 
      ccaaatgtg actttaatt tatcatcag ttgataagg 
      ggaaattat cagcgatgc ctctaatcg gaatgattt 
      tcgggtgca gtctttggg gctcaattg ctgattctc 
      agggacgta aagtcgcaa taatgcttt cgctcctcg 
      ttgattttc tcgcagctt tgatttctt ccttcgctc 
      aaactttca gcttctttt taatttgtc ggttcttca 
      acggattcg gtattatag gggccacta gtattattt 
      tctcttatt tgggcgaat tcgcgtcaa agaaacacc 
      gagatgtga tgttgggtc gtttggaag tgttctgga 
      acgttggag tgatcctac taccaggtg tggcctggg 
      ttctactga accgaacca ctttggact cattcatcg 
      accatggaa atttcagcc cttggcgaa tttttgttc 
      tggtctgca gtatcccaa gcttgatca gtctagtca 
      tgttgtatt tcctaccag agacaccaa aatttctca 
      tttcgaaag ggcaatacg aaaaggcta aactcgttt 
      tccagaagg ttttcacct ttaacactg gatttccct 
      cgtatgctt atccagtca tgactctag aaggagagt 
      acgaaaata ataataatt ctgaaggac tgggcacta 
      aagtgccac ttagggaaa aaatctgtg tcaagatta 
      cgtccattg tgagtgaaa cgcaagtgt tgttcacgc 
      aaccgtatc tgaaatacc tctcagtga cttgttttg 
      cggattttg gcttaatgg caagttact acactttga 
      ttatgtggt ttcccgaaa ttttcgacc gtttcaacc 
      agtttgaag tgactcatc ccaacagta ctgcaagtg 
      tttgttccg tgtccaatg gaagagcgg acgctcttg 
      aagtatttt tccctgaac cttgcaacc cttcaatag 
      ataatcgtg tttttctgg acacgatta ttataggat 
      taagctgtg ttcctacgt cagtatcgc tcagcttct 
      ttatgaaga agttcggca agaaagcgg tattaatta 
      ttggattgg tgatgtcag ggttatcaa ccctctcgc 
      tgaactggg tacaaagtt caactgaaa ctctcgtgc 
      tttcttgtg tttttgaag cactgacca gtattcttg 
      aagctgtga ttttttgct ttattgtcg atttgtttc 
      cgacaaatc ttagggcaa ttgctttag ctgtgactg 
      catcctcag ggagaattg gcgcaattt ttggaaatg 
      tagtttttg gcttgttga tcgattggc aatgttttg 
      taccgattt atctgtttg gctcgctcc tcatagcca 
      gcggaacgc tttgcttgg tcttacctc aaacggaca 
      aatacatgg taatacaat aagaagcca gttttattt 
      tttattttt ttatttttt attttatct tttatttat 
      tcatattta tattctgtt tagttttat ataaaaagt 
      tttattaag tactgtgac gatttttaa tgcccaaac 
      acctacctt tatcccaat ctaaattac aattcttgt 
      aaaaatgta aagctcttt cctcgaatc cacaaaata 
      agcctacac gaaaaatgc gcacaaaaa aaagcccac 
      acggaaaaa agctcgtac cgaaaaatt ctcatactc 
      aaaaaaaaa cattccctg caattgtgc atagattca 
      tgaatttat aagtattct gataacaat aaaaatatt 
      ttagaata
    • >TC032056 RB
      tgtctttat cacgaagcg aaatggacc gctctttct 
      tactaatcc aagaaagaa ctcaccacg attacatcg 
      aaaaagccc aaaccgctg actttgaaa ccgccatcc 
      attactcaa ggtatggaa aattccagg ccttgctcc 
      ttgctgttt gtgggacaa tttacgcaa cttgcgcca 
      tcagctcaa cgactttat cgttcgtct tgccttcgg 
      ccaaatgtg actttaatt tatcatcag ttgataagg 
      ggaaattat cagcgatgc ctctaatcg gaatgattt 
      tcgggtgca gtctttggg gctcaattg ctgattctc 
      agggacgta aagtcgcaa taatgcttt cgctcctcg 
      ttgattttc tcgcagctt tgatttctt ccttcgctc 
      aaactttca gcttctttt taatttgtc ggttcttca 
      acggattcg gtattatag gggccacta gtattattt 
      tctcttatt tgggcgaat tcgcgtcaa agaaacacc 
      gagatgtga tgttgggtc gtttggaag tgttctgga 
      acgttggag tgatcctac taccaggtg tggcctggg 
      ttctactga accgaacca ctttggact cattcatcg 
      accatggaa atttcagcc cttggcgaa tttttgttc 
      tggtctgca gtatcccaa gcttgatca gtctagtca 
      tgttgtatt tcctaccag agacaccaa aatttctca 
      tttcgaaag ggcaatacg aaaaggcta aactcgttt 
      tccagaagg ttttcacct ttaacactg gatttccct 
      cgtatgctt atccagtca tgactctag aaggagagt 
      acgaaaata ataataatt ctgaaggac tgggcacta 
      aagtgccac ttagggaaa aaatctgtg tcaagatta 
      cgtccattg tgagtgaaa cgcaagtgt tgttcacgc 
      aaccgtatc tgaaatacc tctcagtga cttgttttg 
      cggattttg gcttaatgg caagttact acactttga 
      ttatgtggt ttcccgaaa ttttcgacc gtttcaacc 
      agtttgaag tgactcatc ccaacagta ctgcaagtg 
      tttgttccg tgtccaatg gaagagcgg acgctcttg 
      aagtatttt tccctgaac cttgcaacc cttcaatag 
      ataatcgtg tttttctgg acacgatta ttataggat 
      taagctgtg ttcctacgt cagtatcgc tcagcttct 
      ttatgaaga agttcggca agaaagcgg tattaatta 
      ttggattgg tgatgtcag ggttatcaa ccctctcgc 
      tgaactggg tacaaagtt caactgaaa ctctcgtgc 
      tttcttgtg tttttgaag cactgacca gtattcttg 
      aagctgtga ttttttgct ttattgtcg atttgtttc 
      cgacaaatc ttagggcaa ttgctttag ctgtgactg 
      catcctcag ggagaattg gcgcaattt ttggaaatg 
      tagtttttg gcttgttga tcgattggc aatgttttg 
      taccgattt atctgtttg gctcgctcc tcataggta 
      aacaatact atttttaac caattttta acaaatatt 
      tttcagcca gcggaacgc tttgcttgg tcttacctc 
      aaacggaca aatacatgg taatacaat aagaagcca 
      gtt