Status
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Gene information
General information
TC000807 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5891219..5899407
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000807
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00174

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     gena triplet gena triplet posterior mostly not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head bristle partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000807 RA
      MAELLSVPL KKPSEVDIV TPLKNLIQS RYSTADKPE 
DCNEAISEL AKLRNTAIW KAFEKYESS LEIIYGYYD 
QLVSLETKI PAQELQVPF KWKDAFDKG SIFGNRISL 
TIPSLGYEK ICVLFNIAA LQSAVAASQ SIESDESLK 
LAAKLLQQA AGIFNHLKS TVMLTMQQD PTPDLNPDT 
LGALSNLML AQAQEIFVH KAIHDNMKD SIVAKLASQ 
CEDLYGECL KVFQRENLK NNWDKDWIP TIAGKQAAL 
HAVAQFYQS LVCRSNKQI GEEIARLEH SVSLFQTAQ 
QRSGKASLF QELQHKAQR NLAEVKKDN DFIYHERIP 
DIKSLEPIG KAQPAKVLP IAQPMSQGF KDLFSELVP 
VAVHQALAA YDVRKTEIV NTEIGRLRE STTTLNGIL 
ASLNLPAAI EVTDGSSGL PPSIIEKAN TVNQLGGIN 
ELEKMIREL PDALKRNQD ILDETDRML NEEKQADDS 
LRQQFKERW TRTPSDKLN EMFRSNAAK YREIINNAV 
QADKVVREK FESHREGIA LLSKSPTEL QAAVPQGPS 
GNVTNSSAV TTLRQLMEE VETIKAERD VIESELKNT 
NFDMKSTFL SALAKDGAI SEPALSVES LGQAYGPLQ 
KQVRESIEK QESLIARIQ QAHSQFVQE RGSTASGRE 
SKMVQLAGA HDAFRDLLN NLTEGTKFY NDLTQLLVA 
FQNKVSDFC FARKTEKEE LLKDLTQES SRGAVPPPT 
QPSYYTGPD VKKEAPPRP PQPSQPAQP AQPPQAPQP 
SGVPYPVYV QGMPVPYGA STSTPYPAY VPPPMPQGY 
NPYGTMPYP NSYNYPGGF PAAPGYGQQ PPPGSYPQQ 
PPAGYPPQN PGGYPPNPW
    CDS sequence
    • >TC000807 RA
      atggcagag ttgttatcg gttccgttg aaaaaaccg 
tcagaagtg gatatagta acacctctg aaaaattta 
attcagtcc aggtacagt acggctgat aagcccgaa 
gactgcaat gaggcaata agcgagttg gcgaaactt 
cggaacacc gcaatttgg aaagccttc gagaagtat 
gagagttcg ttggagatt atttatgga tattacgac 
cagttggtg tctcttgaa acgaaaatt ccggcccag 
gagttgcaa gttccattt aaatggaag gacgccttt 
gacaagggc tcgatcttt ggcaacaga atcagccta 
acgattcct tcactcggc tatgaaaaa atctgtgtc 
ttgttcaac attgcggct ttgcagagt gcagtggcc 
gccagtcag agtatcgaa agtgatgag tcactcaaa 
ttggcggca aagttgcta cagcaagca gctgggatt 
tttaatcat ttgaaatct accgtaatg ttgactatg 
caacaagac cccactcct gatttaaat cccgatact 
ttgggcgct ttgtccaac ttaatgcta gcgcaagca 
caagagatt tttgttcac aaggcaatc cacgacaat 
atgaaagat tctattgtg gcaaaattg gcgtcgcag 
tgcgaggat ttgtatggt gaatgtttg aaagtgttc 
caaagggaa aatcttaaa aataattgg gacaaagat 
tggatccca acgattgca ggcaaacag gctgctctg 
catgccgtg gctcaattc tatcaaagt ttagtgtgc 
cggagcaac aaacaaatc ggtgaagaa atcgcgagg 
ttggaacat tccgtttca ctgtttcaa acagcccaa 
caacgttca ggcaaagcg tcactcttc caagagtta 
caacacaaa gcgcaaaga aacttggct gaagtaaaa 
aaagacaac gattttatt taccatgaa agaattcct 
gatataaag tcactggaa cctattggc aaagctcaa 
ccggcgaaa gttttaccc atagcacaa ccaatgagc 
caaggattt aaagatttg tttagtgag ttggttcca 
gtggcggta catcaggct ttggctgcg tatgatgtg 
cgtaaaacc gaaattgtt aatactgaa attgggcgt 
cttcgagag tcaactact acgttgaat ggaatttta 
gcttcgttg aatttgccg gctgcgatt gaagttact 
gatggcagt tcaggtcta ccaccttca attatcgaa 
aaagccaat actgttaat cagttgggc ggcataaac 
gaattggaa aaaatgata agggaatta ccagatgcc 
ctcaagagg aatcaagac attctggac gaaactgac 
cggatgttg aatgaagag aaacaagct gatgattct 
ttgcgccaa caattcaaa gaacggtgg actagaaca 
ccgtccgat aaattgaac gaaatgttt cgctcaaat 
gccgcaaag tatcgagag attatcaac aacgctgtt 
caggctgat aaagtcgtc cgtgagaaa ttcgagtct 
caccgcgaa ggcatcgct cttttgagt aaatcgccg 
actgagttg caagcagcg gtccctcaa ggaccatcc 
ggtaacgta acaaattcc tcggctgtt accacttta 
agacagttg atggaagaa gttgaaacg atcaaagca 
gaacgcgac gtcatcgaa tcagaatta aaaaatacc 
aactttgat atgaaatct acattttta tcggcgctg 
gctaaagac ggggctata agtgaaccg gctttgtcc 
gttgaaagt ctgggtcaa gcttacggt ccccttcag 
aagcaagtt agagagagt attgagaaa caggagagt 
cttattgca aggatacaa caagcccat tcccagttt 
gttcaagag agaggttca acggcttct gggagggaa 
agtaaaatg gttcaattg gcgggtgct catgatgca 
tttagagac cttttgaat aatttaaca gaagggaca 
aagttttac aatgatttg actcagtta ttagtcgct 
ttccaaaat aaagtctcc gatttttgc ttcgcacgc 
aagactgag aaagaggag ttgctgaaa gatcttaca 
caggagtca agcagaggg gcggtgccg ccgcccacc 
caaccgtcc tattacaca ggcccagat gtgaagaaa 
gaggcccct ccgaggccc ccacagcct tcgcagcct 
gcgcagcct gcgcagccc ccgcaagcc ccgcagccc 
tctggggtc ccatatccc gtttacgtc caaggaatg 
cctgtacca tatggggcg tccacttca accccctat 
cctgcttac gtgccaccg cccatgcct caaggttac 
aacccatat ggcacgatg ccgtatccc aattcgtat 
aattatcca ggtggattt ccagctgct ccaggttat 
gggcaacag ccacctccg gggagttat ccccaacaa 
ccaccggcg ggatatcct ccacaaaat ccagggggc 
tatccacca aatccctgg tag
    mRNA sequence
    • >TC000807 RA
      ttccagatg agatcatca atgttttgg tgttcatgt 
gattcctga atcagtgca actaattag tgattaatt 
attttgtgt atttgcact tgtgtgtat tattaatat 
tttcccggt cttttgaca cttcgctgt caagttgac 
agttgtaat aaaccgtga aaatggcag agttgttat 
cggttccgt tgaaaaaac cgtcagaag tggatatag 
taacacctc tgaaaaatt taattcagt ccaggtaca 
gtacggctg ataagcccg aagactgca atgaggcaa 
taagcgagt tggcgaaac ttcggaaca ccgcaattt 
ggaaagcct tcgagaagt atgagagtt cgttggaga 
ttatttatg gatattacg accagttgg tgtctcttg 
aaacgaaaa ttccggccc aggagttgc aagttccat 
ttaaatgga aggacgcct ttgacaagg gctcgatct 
ttggcaaca gaatcagcc taacgattc cttcactcg 
gctatgaaa aaatctgtg tcttgttca acattgcgg 
ctttgcaga gtgcagtgg ccgccagtc agagtatcg 
aaagtgatg agtcactca aattggcgg caaagttgc 
tacagcaag cagctggga tttttaatc atttgaaat 
ctaccgtaa tgttgacta tgcaacaag accccactc 
ctgatttaa atcccgata ctttgggcg ctttgtcca 
acttaatgc tagcgcaag cacaagaga tttttgttc 
acaaggcaa tccacgaca atatgaaag attctattg 
tggcaaaat tggcgtcgc agtgcgagg atttgtatg 
gtgaatgtt tgaaagtgt tccaaaggg aaaatctta 
aaaataatt gggacaaag attggatcc caacgattg 
caggcaaac aggctgctc tgcatgccg tggctcaat 
tctatcaaa gtttagtgt gccggagca acaaacaaa 
tcggtgaag aaatcgcga ggttggaac attccgttt 
cactgtttc aaacagccc aacaacgtt caggcaaag 
cgtcactct tccaagagt tacaacaca aagcgcaaa 
gaaacttgg ctgaagtaa aaaaagaca acgatttta 
tttaccatg aaagaattc ctgatataa agtcactgg 
aacctattg gcaaagctc aaccggcga aagttttac 
ccatagcac aaccaatga gccaaggat ttaaagatt 
tgtttagtg agttggttc cagtggcgg tacatcagg 
ctttggctg cgtatgatg tgcgtaaaa ccgaaattg 
ttaatactg aaattgggc gtcttcgag agtcaacta 
ctacgttga atggaattt tagcttcgt tgaatttgc 
cggctgcga ttgaagtta ctgatggca gttcaggtc 
taccacctt caattatcg aaaaagcca atactgtta 
atcagttgg gcggcataa acgaattgg aaaaaatga 
taagggaat taccagatg ccctcaaga ggaatcaag 
acattctgg acgaaactg accggatgt tgaatgaag 
agaaacaag ctgatgatt ctttgcgcc aacaattca 
aagaacggt ggactagaa caccgtccg ataaattga 
acgaaatgt ttcgctcaa atgccgcaa agtatcgag 
agattatca acaacgctg ttcaggctg ataaagtcg 
tccgtgaga aattcgagt ctcaccgcg aaggcatcg 
ctcttttga gtaaatcgc cgactgagt tgcaagcag 
cggtccctc aaggaccat ccggtaacg taacaaatt 
cctcggctg ttaccactt taagacagt tgatggaag 
aagttgaaa cgatcaaag cagaacgcg acgtcatcg 
aatcagaat taaaaaata ccaactttg atatgaaat 
ctacatttt tatcggcgc tggctaaag acggggcta 
taagtgaac cggctttgt ccgttgaaa gtctgggtc 
aagcttacg gtccccttc agaagcaag ttagagaga 
gtattgaga aacaggaga gtcttattg caaggatac 
aacaagccc attcccagt ttgttcaag agagaggtt 
caacggctt ctgggaggg aaagtaaaa tggttcaat 
tggcgggtg ctcatgatg catttagag accttttga 
ataatttaa cagaaggga caaagtttt acaatgatt 
tgactcagt tattagtcg ctttccaaa ataaagtct 
ccgattttt gcttcgcac gcaagactg agaaagagg 
agttgctga aagatctta cacaggagt caagcagag 
gggcggtgc cgccgccca cccaaccgt cctattaca 
caggcccag atgtgaaga aagaggccc ctccgaggc 
ccccacagc cttcgcagc ctgcgcagc ctgcgcagc 
ccccgcaag ccccgcagc cctctgggg tcccatatc 
ccgtttacg tccaaggaa tgcctgtac catatgggg 
cgtccactt caaccccct atcctgctt acgtgccac 
cgcccatgc ctcaaggtt acaacccat atggcacga 
tgccgtatc ccaattcgt ataattatc caggtggat 
ttccagctg ctccaggtt atgggcaac agccacctc 
cggggagtt atccccaac aaccaccgg cgggatatc 
ctccacaaa atccagggg gctatccac caaatccct 
ggtagtgct atctacagc cagtgttgt gttaagttt 
agatcaggt aaatttcgt catcaagca atttgttaa 
tttaaaaat taattatca cgagtctat atgaatatt 
atgatgtat ctttatcta agtttatgt agagagcaa 
ttattgtta accgaatct gtcaattct atctaagtt 
gcaaaagtc atttttgta tcataggga aatattttt 
ataatatta ttgatgtta attattatg gattgtaag 
gatattagt gcctccgtt tgtattaat cttgcaaaa 
taaatgatt accaaatat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY
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