Status
Please wait ...
Gene information
General information
TC000807 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5891219..5899407
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000807
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00174

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    gena triplet gena triplet posterior mostly not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head bristle partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000807 RA
      MAELLSVPL KKPSEVDIV TPLKNLIQS RYSTADKPE 
      DCNEAISEL AKLRNTAIW KAFEKYESS LEIIYGYYD 
      QLVSLETKI PAQELQVPF KWKDAFDKG SIFGNRISL 
      TIPSLGYEK ICVLFNIAA LQSAVAASQ SIESDESLK 
      LAAKLLQQA AGIFNHLKS TVMLTMQQD PTPDLNPDT 
      LGALSNLML AQAQEIFVH KAIHDNMKD SIVAKLASQ 
      CEDLYGECL KVFQRENLK NNWDKDWIP TIAGKQAAL 
      HAVAQFYQS LVCRSNKQI GEEIARLEH SVSLFQTAQ 
      QRSGKASLF QELQHKAQR NLAEVKKDN DFIYHERIP 
      DIKSLEPIG KAQPAKVLP IAQPMSQGF KDLFSELVP 
      VAVHQALAA YDVRKTEIV NTEIGRLRE STTTLNGIL 
      ASLNLPAAI EVTDGSSGL PPSIIEKAN TVNQLGGIN 
      ELEKMIREL PDALKRNQD ILDETDRML NEEKQADDS 
      LRQQFKERW TRTPSDKLN EMFRSNAAK YREIINNAV 
      QADKVVREK FESHREGIA LLSKSPTEL QAAVPQGPS 
      GNVTNSSAV TTLRQLMEE VETIKAERD VIESELKNT 
      NFDMKSTFL SALAKDGAI SEPALSVES LGQAYGPLQ 
      KQVRESIEK QESLIARIQ QAHSQFVQE RGSTASGRE 
      SKMVQLAGA HDAFRDLLN NLTEGTKFY NDLTQLLVA 
      FQNKVSDFC FARKTEKEE LLKDLTQES SRGAVPPPT 
      QPSYYTGPD VKKEAPPRP PQPSQPAQP AQPPQAPQP 
      SGVPYPVYV QGMPVPYGA STSTPYPAY VPPPMPQGY 
      NPYGTMPYP NSYNYPGGF PAAPGYGQQ PPPGSYPQQ 
      PPAGYPPQN PGGYPPNPW
    CDS sequence
    • >TC000807 RA
      atggcagag ttgttatcg gttccgttg aaaaaaccg 
      tcagaagtg gatatagta acacctctg aaaaattta 
      attcagtcc aggtacagt acggctgat aagcccgaa 
      gactgcaat gaggcaata agcgagttg gcgaaactt 
      cggaacacc gcaatttgg aaagccttc gagaagtat 
      gagagttcg ttggagatt atttatgga tattacgac 
      cagttggtg tctcttgaa acgaaaatt ccggcccag 
      gagttgcaa gttccattt aaatggaag gacgccttt 
      gacaagggc tcgatcttt ggcaacaga atcagccta 
      acgattcct tcactcggc tatgaaaaa atctgtgtc 
      ttgttcaac attgcggct ttgcagagt gcagtggcc 
      gccagtcag agtatcgaa agtgatgag tcactcaaa 
      ttggcggca aagttgcta cagcaagca gctgggatt 
      tttaatcat ttgaaatct accgtaatg ttgactatg 
      caacaagac cccactcct gatttaaat cccgatact 
      ttgggcgct ttgtccaac ttaatgcta gcgcaagca 
      caagagatt tttgttcac aaggcaatc cacgacaat 
      atgaaagat tctattgtg gcaaaattg gcgtcgcag 
      tgcgaggat ttgtatggt gaatgtttg aaagtgttc 
      caaagggaa aatcttaaa aataattgg gacaaagat 
      tggatccca acgattgca ggcaaacag gctgctctg 
      catgccgtg gctcaattc tatcaaagt ttagtgtgc 
      cggagcaac aaacaaatc ggtgaagaa atcgcgagg 
      ttggaacat tccgtttca ctgtttcaa acagcccaa 
      caacgttca ggcaaagcg tcactcttc caagagtta 
      caacacaaa gcgcaaaga aacttggct gaagtaaaa 
      aaagacaac gattttatt taccatgaa agaattcct 
      gatataaag tcactggaa cctattggc aaagctcaa 
      ccggcgaaa gttttaccc atagcacaa ccaatgagc 
      caaggattt aaagatttg tttagtgag ttggttcca 
      gtggcggta catcaggct ttggctgcg tatgatgtg 
      cgtaaaacc gaaattgtt aatactgaa attgggcgt 
      cttcgagag tcaactact acgttgaat ggaatttta 
      gcttcgttg aatttgccg gctgcgatt gaagttact 
      gatggcagt tcaggtcta ccaccttca attatcgaa 
      aaagccaat actgttaat cagttgggc ggcataaac 
      gaattggaa aaaatgata agggaatta ccagatgcc 
      ctcaagagg aatcaagac attctggac gaaactgac 
      cggatgttg aatgaagag aaacaagct gatgattct 
      ttgcgccaa caattcaaa gaacggtgg actagaaca 
      ccgtccgat aaattgaac gaaatgttt cgctcaaat 
      gccgcaaag tatcgagag attatcaac aacgctgtt 
      caggctgat aaagtcgtc cgtgagaaa ttcgagtct 
      caccgcgaa ggcatcgct cttttgagt aaatcgccg 
      actgagttg caagcagcg gtccctcaa ggaccatcc 
      ggtaacgta acaaattcc tcggctgtt accacttta 
      agacagttg atggaagaa gttgaaacg atcaaagca 
      gaacgcgac gtcatcgaa tcagaatta aaaaatacc 
      aactttgat atgaaatct acattttta tcggcgctg 
      gctaaagac ggggctata agtgaaccg gctttgtcc 
      gttgaaagt ctgggtcaa gcttacggt ccccttcag 
      aagcaagtt agagagagt attgagaaa caggagagt 
      cttattgca aggatacaa caagcccat tcccagttt 
      gttcaagag agaggttca acggcttct gggagggaa 
      agtaaaatg gttcaattg gcgggtgct catgatgca 
      tttagagac cttttgaat aatttaaca gaagggaca 
      aagttttac aatgatttg actcagtta ttagtcgct 
      ttccaaaat aaagtctcc gatttttgc ttcgcacgc 
      aagactgag aaagaggag ttgctgaaa gatcttaca 
      caggagtca agcagaggg gcggtgccg ccgcccacc 
      caaccgtcc tattacaca ggcccagat gtgaagaaa 
      gaggcccct ccgaggccc ccacagcct tcgcagcct 
      gcgcagcct gcgcagccc ccgcaagcc ccgcagccc 
      tctggggtc ccatatccc gtttacgtc caaggaatg 
      cctgtacca tatggggcg tccacttca accccctat 
      cctgcttac gtgccaccg cccatgcct caaggttac 
      aacccatat ggcacgatg ccgtatccc aattcgtat 
      aattatcca ggtggattt ccagctgct ccaggttat 
      gggcaacag ccacctccg gggagttat ccccaacaa 
      ccaccggcg ggatatcct ccacaaaat ccagggggc 
      tatccacca aatccctgg tag
    mRNA sequence
    • >TC000807 RA
      ttccagatg agatcatca atgttttgg tgttcatgt 
      gattcctga atcagtgca actaattag tgattaatt 
      attttgtgt atttgcact tgtgtgtat tattaatat 
      tttcccggt cttttgaca cttcgctgt caagttgac 
      agttgtaat aaaccgtga aaatggcag agttgttat 
      cggttccgt tgaaaaaac cgtcagaag tggatatag 
      taacacctc tgaaaaatt taattcagt ccaggtaca 
      gtacggctg ataagcccg aagactgca atgaggcaa 
      taagcgagt tggcgaaac ttcggaaca ccgcaattt 
      ggaaagcct tcgagaagt atgagagtt cgttggaga 
      ttatttatg gatattacg accagttgg tgtctcttg 
      aaacgaaaa ttccggccc aggagttgc aagttccat 
      ttaaatgga aggacgcct ttgacaagg gctcgatct 
      ttggcaaca gaatcagcc taacgattc cttcactcg 
      gctatgaaa aaatctgtg tcttgttca acattgcgg 
      ctttgcaga gtgcagtgg ccgccagtc agagtatcg 
      aaagtgatg agtcactca aattggcgg caaagttgc 
      tacagcaag cagctggga tttttaatc atttgaaat 
      ctaccgtaa tgttgacta tgcaacaag accccactc 
      ctgatttaa atcccgata ctttgggcg ctttgtcca 
      acttaatgc tagcgcaag cacaagaga tttttgttc 
      acaaggcaa tccacgaca atatgaaag attctattg 
      tggcaaaat tggcgtcgc agtgcgagg atttgtatg 
      gtgaatgtt tgaaagtgt tccaaaggg aaaatctta 
      aaaataatt gggacaaag attggatcc caacgattg 
      caggcaaac aggctgctc tgcatgccg tggctcaat 
      tctatcaaa gtttagtgt gccggagca acaaacaaa 
      tcggtgaag aaatcgcga ggttggaac attccgttt 
      cactgtttc aaacagccc aacaacgtt caggcaaag 
      cgtcactct tccaagagt tacaacaca aagcgcaaa 
      gaaacttgg ctgaagtaa aaaaagaca acgatttta 
      tttaccatg aaagaattc ctgatataa agtcactgg 
      aacctattg gcaaagctc aaccggcga aagttttac 
      ccatagcac aaccaatga gccaaggat ttaaagatt 
      tgtttagtg agttggttc cagtggcgg tacatcagg 
      ctttggctg cgtatgatg tgcgtaaaa ccgaaattg 
      ttaatactg aaattgggc gtcttcgag agtcaacta 
      ctacgttga atggaattt tagcttcgt tgaatttgc 
      cggctgcga ttgaagtta ctgatggca gttcaggtc 
      taccacctt caattatcg aaaaagcca atactgtta 
      atcagttgg gcggcataa acgaattgg aaaaaatga 
      taagggaat taccagatg ccctcaaga ggaatcaag 
      acattctgg acgaaactg accggatgt tgaatgaag 
      agaaacaag ctgatgatt ctttgcgcc aacaattca 
      aagaacggt ggactagaa caccgtccg ataaattga 
      acgaaatgt ttcgctcaa atgccgcaa agtatcgag 
      agattatca acaacgctg ttcaggctg ataaagtcg 
      tccgtgaga aattcgagt ctcaccgcg aaggcatcg 
      ctcttttga gtaaatcgc cgactgagt tgcaagcag 
      cggtccctc aaggaccat ccggtaacg taacaaatt 
      cctcggctg ttaccactt taagacagt tgatggaag 
      aagttgaaa cgatcaaag cagaacgcg acgtcatcg 
      aatcagaat taaaaaata ccaactttg atatgaaat 
      ctacatttt tatcggcgc tggctaaag acggggcta 
      taagtgaac cggctttgt ccgttgaaa gtctgggtc 
      aagcttacg gtccccttc agaagcaag ttagagaga 
      gtattgaga aacaggaga gtcttattg caaggatac 
      aacaagccc attcccagt ttgttcaag agagaggtt 
      caacggctt ctgggaggg aaagtaaaa tggttcaat 
      tggcgggtg ctcatgatg catttagag accttttga 
      ataatttaa cagaaggga caaagtttt acaatgatt 
      tgactcagt tattagtcg ctttccaaa ataaagtct 
      ccgattttt gcttcgcac gcaagactg agaaagagg 
      agttgctga aagatctta cacaggagt caagcagag 
      gggcggtgc cgccgccca cccaaccgt cctattaca 
      caggcccag atgtgaaga aagaggccc ctccgaggc 
      ccccacagc cttcgcagc ctgcgcagc ctgcgcagc 
      ccccgcaag ccccgcagc cctctgggg tcccatatc 
      ccgtttacg tccaaggaa tgcctgtac catatgggg 
      cgtccactt caaccccct atcctgctt acgtgccac 
      cgcccatgc ctcaaggtt acaacccat atggcacga 
      tgccgtatc ccaattcgt ataattatc caggtggat 
      ttccagctg ctccaggtt atgggcaac agccacctc 
      cggggagtt atccccaac aaccaccgg cgggatatc 
      ctccacaaa atccagggg gctatccac caaatccct 
      ggtagtgct atctacagc cagtgttgt gttaagttt 
      agatcaggt aaatttcgt catcaagca atttgttaa 
      tttaaaaat taattatca cgagtctat atgaatatt 
      atgatgtat ctttatcta agtttatgt agagagcaa 
      ttattgtta accgaatct gtcaattct atctaagtt 
      gcaaaagtc atttttgta tcataggga aatattttt 
      ataatatta ttgatgtta attattatg gattgtaag 
      gatattagt gcctccgtt tgtattaat cttgcaaaa 
      taaatgatt accaaatat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY