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Gene information
General information
TC000798 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5829889..5831968
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000798
    iBeetle screen iB_00172

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000798 RA
      MTFLTIVLC LLPLTFGVS KTFDLTVLH INDFHARFE 
      ETNDEGGSC KSDQCIGGF SRTFNVINQ SLTQHPDSI 
      LLNAGDNFQ GTLWYNFFK WNVTQYFLN ELPFDAIVL 
      GNHEFDDGI KGVVPFIKS LKAPVIASN IDDSQEPDL 
      QNIYQKSVV VERNGKKIG IIGVLIHTC NEISNTEKL 
      SFLDESESV NTEAERLVK EEGVFTNIV LSHSGYDIE 
      QEIARKATK ASKISLVVG GHSHTFLYT GEPVPGPAT 
      PSGPYPTII NRTEDDRQV LVVQASCYA RYLGNITVS 
      YDENGNCVG WSGEPIFLD TDTPQDKII NKELQPWKD 
      AVDELGNRV LGQTLVTLD KTMCYQSEC LLGNFVTDV 
      MVYSYIALA DNTSYWTYA SLAVFNVGG LRTTIERGD 
      ITYSDMMTA QPFENTFDV GEIEGKYIK EMFEFSMTP 
      YSYGRTYAD VNLLQISGF HIVANLSQP IGSRIQSIK 
      ARCNECNIP IYEDLDLNK TYRLIVPVF LRNGGDGFT 
      VIRDNLKNV KVGRVDIDV FVEYLGAKS PVFEEIDGR 
      IEFVGDVTI NCRNLDS
    CDS sequence
    • >TC000798 RA
      atgactttc ctaacaatt gttctgtgt ttacttccc 
      ttgactttt ggtgtctct aagacgttt gacttgacg 
      gttttgcac ataaatgac ttccatgcc agatttgag 
      gaaacgaac gatgaagga ggttcttgc aaaagtgat 
      caatgtatc ggcggattt tctcgaacg tttaatgta 
      ataaatcag tctttgact caacatcct gattcaatt 
      ttactaaac gcgggagat aattttcaa ggaacgctg 
      tggtataat ttcttcaaa tggaacgtg acacaatat 
      ttcctcaac gaattgccg tttgatgca attgtgttg 
      ggtaaccac gagtttgat gatggtatt aagggagtt 
      gtcccattc atcaaaagc ttaaaagct ccagtcatt 
      gcatcaaac attgacgat agccaagaa ccagactta 
      caaaatatt taccaaaaa agcgtggtc gttgaaaga 
      aacggtaaa aaaattggg attattggc gtattgatt 
      cacacatgt aatgaaatc tcaaacact gaaaaactg 
      agctttcta gacgagtct gaatcagtt aataccgaa 
      gctgaacga ttagttaag gaagaggga gttttcact 
      aacatagtt ttgtctcat tcgggttac gacattgaa 
      caagaaatt gcaagaaaa gcaactaag gcgtctaaa 
      ataagtttg gttgttggc ggacattcg cacacattt 
      ttatacaca ggagaacct gttcctggc cctgcgact 
      ccgtcagga ccgtaccca acaattata aatcgaact 
      gaagacgac agacaagtt ctagtagtc caagcttct 
      tgttatgcc agatatctt ggaaatata acggtaagt 
      tacgacgaa aatgggaat tgtgtcggt tggagtgga 
      gaaccaata tttctagat acggacaca cctcaagac 
      aagataatt aacaaagaa ctacaacct tggaaagat 
      gcagttgat gaattaggt aatagagtt cttggacaa 
      actttggta acactagac aagacaatg tgttatcaa 
      tccgaatgt ttgcttggt aattttgtg accgacgtg 
      atggtttat agttacata gcattggct gacaacacg 
      tcttattgg acttatgcc tctttggcc gttttcaac 
      gttggaggt ttgagaact acgatagaa cgtggagat 
      ataacttac agcgacatg atgacagcc caaccgttc 
      gaaaacacg ttcgatgtt ggcgaaatt gaaggaaaa 
      tatatcaaa gaaatgttt gagttcagt atgactcca 
      tacagctac ggacgaact tacgcagac gtaaactta 
      ttacaaatt tcaggtttt cacattgtt gcaaattta 
      tcacaaccg atcggctcc cggattcaa tcaatcaaa 
      gctcgttgc aacgaatgc aacatcccg atttacgaa 
      gacttggat ttgaataaa acttaccgc ttaattgtg 
      ccagttttt ctgagaaat ggtggagac ggtttcacc 
      gttattcga gacaatttg aaaaatgtt aaagtcgga 
      agagtggac attgatgtt tttgttgag tatttaggg 
      gcgaaatcg ccagttttt gaggaaatt gatgggaga 
      atcgagttc gttggagac gtgacgatt aattgcaga 
      aatttggat tcctaa
    mRNA sequence
    • >TC000798 RA
      gtctagagt taaaaatga ctttcctaa caattgttc 
      tgtgtttac ttcccttga cttttggtg tctctaaga 
      cgtttgact tgacggttt tgcacataa atgacttcc 
      atgccagat ttgaggaaa cgaacgatg aaggaggtt 
      cttgcaaaa gtgatcaat gtatcggcg gattttctc 
      gaacgttta atgtaataa atcagtctt tgactcaac 
      atcctgatt caattttac taaacgcgg gagataatt 
      ttcaaggaa cgctgtggt ataatttct tcaaatgga 
      acgtgacac aatatttcc tcaacgaat tgccgtttg 
      atgcaattg tgttgggta accacgagt ttgatgatg 
      gtattaagg gagttgtcc cattcatca aaagcttaa 
      aagctccag tcattgcat caaacattg acgatagcc 
      aagaaccag acttacaaa atatttacc aaaaaagcg 
      tggtcgttg aaagaaacg gtaaaaaaa ttgggatta 
      ttggcgtat tgattcaca catgtaatg aaatctcaa 
      acactgaaa aactgagct ttctagacg agtctgaat 
      cagttaata ccgaagctg aacgattag ttaaggaag 
      agggagttt tcactaaca tagttttgt ctcattcgg 
      gttacgaca ttgaacaag aaattgcaa gaaaagcaa 
      ctaaggcgt ctaaaataa gtttggttg ttggcggac 
      attcgcaca catttttat acacaggag aacctgttc 
      ctggccctg cgactccgt caggaccgt acccaacaa 
      ttataaatc gaactgaag acgacagac aagttctag 
      tagtccaag cttcttgtt atgccagat atcttggaa 
      atataacgg taagttacg acgaaaatg ggaattgtg 
      tcggttgga gtggagaac caatatttc tagatacgg 
      acacacctc aagacaaga taattaaca aagaactac 
      aaccttgga aagatgcag ttgatgaat taggtaata 
      gagttcttg gacaaactt tggtaacac tagacaaga 
      caatgtgtt atcaatccg aatgtttgc ttggtaatt 
      ttgtgaccg acgtgatgg tttatagtt acatagcat 
      tggctgaca acacgtctt attggactt atgcctctt 
      tggccgttt tcaacgttg gaggtttga gaactacga 
      tagaacgtg gagatataa cttacagcg acatgatga 
      cagcccaac cgttcgaaa acacgttcg atgttggcg 
      aaattgaag gaaaatata tcaaagaaa tgtttgagt 
      tcagtatga ctccataca gctacggac gaacttacg 
      cagacgtaa acttattac aaatttcag gttttcaca 
      ttgttgcaa atttatcac aaccgatcg gctcccgga 
      ttcaatcaa tcaaagctc gttgcaacg aatgcaaca 
      tcccgattt acgaagact tggatttga ataaaactt 
      accgcttaa ttgtgccag tttttctga gaaatggtg 
      gagacggtt tcaccgtta ttcgagaca atttgaaaa 
      atgttaaag tcggaagag tggacattg atgtttttg 
      ttgagtatt taggggcga aatcgccag tttttgagg 
      aaattgatg ggagaatcg agttcgttg gagacgtga 
      cgattaatt gcagaaatt tggattcct aatactatg 
      taatttttt gtaacagaa ataaaatac acgcttaac 
      aatatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY