Status
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Gene information
General information
TC000759 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5857124..5861258
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000759
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00169

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 9 (19 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000759 RA
      MKNWDVLCI FMVVRLASV FLVQTFFVP DEYWQSLEV 
      AHGISFGYG YLTWEWRAG IRSYIYPLI FSCFYSLLK 
      YLQFDDPLL LIYGPRILQ ALLSAYADL CFYQWCGAK 
      KWAVFSIAT SWFWFYTGS RTLITFECA LSTIALSKF 
      PWLGKGKDE NLKFIWIVA ILSVIRPTA AVIWSPLCV 
      YHLMITKYK ISSLIITRY IPISLIVLA ASILLDSLS 
      HGYFIVTPY EFLKFNIIQ DVGTHYGSQ PWFWYLSAG 
      FPAILGIHF LPFVLATLV VIKHRSVHP NELALLGTV 
      VFSLTVYSF LPHKEFRFL LPLLPIALF ISSRFLSVW 
      SRKASNVML WTVALILFV GNVGPSYYL GYVHQRGTL 
      DVMGPLREI AIKDPKNTN LLFLMPCHS TPLYSHLHV 
      NVTARFLTC EPNFNNTSG SYVDEADQF YSSPSVWLR 
      RNYPANGTL PSHIICFDT LKPMISDIM SRYKTTHEI 
      PHTNFPTSR VGYNVLINE RIGN
    CDS sequence
    • >TC000759 RA
      atgaagaat tgggacgtt ctttgcatt tttatggtc 
      gtaagactt gcctcagta tttctagta caaactttc 
      ttcgtacct gacgaatat tggcagtcg ctggaggtt 
      gcccatggt atttctttt ggatatggg tacctcaca 
      tgggagtgg cgtgcagga atcaggagt tacatttat 
      ccgttaata tttagttgt ttttacagt ttgctcaag 
      tatttgcaa tttgacgat cctttatta ctgatctac 
      gggccacgt attttgcaa gcccttttg agcgcttat 
      gccgatttg tgtttttac cagtggtgt ggggcaaaa 
      aaatgggct gtgttcagc attgcaacg tcgtggttt 
      tggttttac accggatca agaaccttg attactttt 
      gaatgtgct ttgagcacg atagcgctt tccaaattt 
      ccctggctg gggaaaggc aaagacgaa aatttaaaa 
      tttatttgg atagttgcc atactttca gtaatacgc 
      cccacagct gcagtaata tggtcacca ctttgcgtt 
      tatcattta atgataacc aaatataaa atttcatct 
      ctgattatc acgcgctac attcccatc agtcttata 
      gtactagcc gcgtccatt cttttagac agtctctct 
      cacggatat tttatcgta actccttac gagttcctc 
      aaatttaac ataatccaa gatgtgggc acacattac 
      ggctcccag ccttggttt tggtactta tctgcaggt 
      ttccctgca attctcgga atccacttt ttgcccttc 
      gtgttggcc acacttgtc gtaataaaa caccgcagt 
      gtgcacccc aacgaattg gccctgctt ggtactgtg 
      gttttctcg ttaacagtt tacagtttt ctaccgcat 
      aaagaattc agatttttg ctgcctctg ctaccgata 
      gcgttattt atttcgtcc cgattttta tcagtttgg 
      agtcgcaaa gctagtaat gttatgctg tggacggtt 
      gcgttaatt ttatttgtg ggaaatgtg ggcccgtcc 
      tattatttg ggctacgtc catcagcgt ggtacttta 
      gacgtgatg ggtccttta cgagagata gcgataaaa 
      gatccgaaa aatacgaat ttgttgttt ttaatgcct 
      tgccattct acacctttg tacagccac ttgcatgta 
      aatgtgact gctagattt ttaacgtgt gagccgaat 
      tttaataac actagtggg agttatgtt gacgaagct 
      gatcagttt tatagtagt cctagtgtt tggctacgg 
      aggaattat ccagctaat gggacttta ccgtctcat 
      attatttgt ttcgatact ttgaaaccc atgatcagt 
      gatataatg agcaggtat aagacaacg catgaaatt 
      ccacacact aattttccg acgtcaaga gtgggatat 
      aacgtttta ataaatgaa agaattggt aattga
    mRNA sequence
    • >TC000759 RA
      cgggaggtg gtctcccga gttatttat acattttat 
      tttggtaaa aatacgcgg agacaaaaa tatcggagt 
      tttagaatt agaatgtgt ccgtaaagt ccagtgtct 
      cattcacca tttttgtaa aaatttccg atgaaaaat 
      gaagaattg ggacgttct ttgcatttt tatggtcgt 
      aagacttgc ctcagtatt tctagtaca aactttctt 
      cgtacctga cgaatattg gcagtcgct ggaggttgc 
      ccatggtat ttcttttgg atatgggta cctcacatg 
      ggagtggcg tgcaggaat caggagtta catttatcc 
      gttaatatt tagttgttt ttacagttt gctcaagta 
      tttgcaatt tgacgatcc tttattact gatctacgg 
      gccacgtat tttgcaagc ccttttgag cgcttatgc 
      cgatttgtg tttttacca gtggtgtgg ggcaaaaaa 
      atgggctgt gttcagcat tgcaacgtc gtggttttg 
      gttttacac cggatcaag aaccttgat tacttttga 
      atgtgcttt gagcacgat agcgctttc caaatttcc 
      ctggctggg gaaaggcaa agacgaaaa tttaaaatt 
      tatttggat agttgccat actttcagt aatacgccc 
      cacagctgc agtaatatg gtcaccact ttgcgttta 
      tcatttaat gataaccaa atataaaat ttcatctct 
      gattatcac gcgctacat tcccatcag tcttatagt 
      actagccgc gtccattct tttagacag tctctctca 
      cggatattt tatcgtaac tccttacga gttcctcaa 
      atttaacat aatccaaga tgtgggcac acattacgg 
      ctcccagcc ttggttttg gtacttatc tgcaggttt 
      ccctgcaat tctcggaat ccacttttt gcccttcgt 
      gttggccac acttgtcgt aataaaaca ccgcagtgt 
      gcaccccaa cgaattggc cctgcttgg tactgtggt 
      tttctcgtt aacagttta cagttttct accgcataa 
      agaattcag atttttgct gcctctgct accgatagc 
      gttatttat ttcgtcccg atttttatc agtttggag 
      tcgcaaagc tagtaatgt tatgctgtg gacggttgc 
      gttaatttt atttgtggg aaatgtggg cccgtccta 
      ttatttggg ctacgtcca tcagcgtgg tactttaga 
      cgtgatggg tcctttacg agagatagc gataaaaga 
      tccgaaaaa tacgaattt gttgttttt aatgccttg 
      ccattctac acctttgta cagccactt gcatgtaaa 
      tgtgactgc tagattttt aacgtgtga gccgaattt 
      taataacac tagtgggag ttatgttga cgaagctga 
      tcagtttta tagtagtcc tagtgtttg gctacggag 
      gaattatcc agctaatgg gactttacc gtctcatat 
      tatttgttt cgatacttt gaaacccat gatcagtga 
      tataatgag caggtataa gacaacgca tgaaattcc 
      acacactaa ttttccgac gtcaagagt gggatataa 
      cgttttaat aaatgaaag aattggtaa ttgattttt 
      attgtttta ttttgtatt gctcaagtt aggtcactg 
      ccatgttgt tattatagt taaataaat gtttgaatg 
      aat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY