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Gene information
General information
TC000753 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid7: 606284..615544
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000753
    iBeetle screen iB_00168
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 4

    Adult morphology
    elytra anterior shape irregular - Number of animals showing the phenotype: 5 (22 dpi)
    Days post injection


    Comment: with dorsal median depression

    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 5 - Number of animals showing the phenotype: 2
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000753 RA
      MLVLLFQAP LQNMPISDF RKKKLLYVF 
      NVFFDVNQS GTIDRKDFE LAIEKICSL 
      RGWSSGTEH YKKTYDSMI QIWDGLRQR 
      ADSNKDGQV SVEEWASMW NEYAKSPEN 
      ALEWQTQYL KFMFDLEDA SGDGSIDVD 
      EFTSVCSCY GLEPSECKE AFQKMSCGK 
      KEVNYEQFE ALWKQFFTS ENPSDPGNF 
      IFGKTKF
    CDS sequence
    • >TC000753 RA
      atgttggtt ttgttgttt caggcacca ctacaaaac 
      atgcctatc tcggatttt cgcaagaag aagctcctc 
      tacgttttc aacgtattt ttcgacgtc aaccagagt 
      ggaaccatt gacagaaag gatttcgag ttggcgatc 
      gagaaaata tgctctcta cgggggtgg tcttcgggg 
      accgaacac tataaaaaa acctacgac agcatgata 
      caaatttgg gacggcctg agacaaagg gctgactca 
      aacaaggat ggacaggtc agcgtcgag gaatgggcc 
      tcaatgtgg aacgaatac gccaagagc ccggaaaat 
      gcattagaa tggcaaaca cagtatttg aagttcatg 
      tttgaccta gaagacgcc agtggtgat ggcagcatc 
      gacgttgat gaatttaca agtgtgtgc tcctgttac 
      ggactggaa ccgtccgag tgtaaagag gcttttcag 
      aaaatgtct tgtggcaag aaagaagtc aactatgaa 
      cagttcgag gctctgtgg aagcagttt ttcacttcg 
      gaaaatccc tccgatcct gggaatttt atttttggc 
      aaaactaaa ttttaa
    mRNA sequence
    • >TC000753 RA
      atgacccag aaacgctga cttttgttg agctttttc 
      caactttta tttgcagtg acttgctct aaccatgtt 
      ggttttgtt gtttcaggc accactaca aaacatgcc 
      tatctcgga ttttcgcaa gaagaagct cctctacgt 
      tttcaacgt atttttcga cgtcaacca gagtggaac 
      cattgacag aaaggattt cgagttggc gatcgagaa 
      aatatgctc tctacgggg gtggtcttc ggggaccga 
      acactataa aaaaaccta cgacagcat gatacaaat 
      ttgggacgg cctgagaca aagggctga ctcaaacaa 
      ggatggaca ggtcagcgt cgaggaatg ggcctcaat 
      gtggaacga atacgccaa gagcccgga aaatgcatt 
      agaatggca aacacagta tttgaagtt catgtttga 
      cctagaaga cgccagtgg tgatggcag catcgacgt 
      tgatgaatt tacaagtgt gtgctcctg ttacggact 
      ggaaccgtc cgagtgtaa agaggcttt tcagaaaat 
      gtcttgtgg caagaaaga agtcaacta tgaacagtt 
      cgaggctct gtggaagca gtttttcac ttcggaaaa 
      tccctccga tcctgggaa ttttatttt tggcaaaac 
      taaatttta accgatttg tgtttgaca tggttgtat 
      aagcgtctc gtaccttct ccttcaaag tgtcgctat 
      gagaacact gagatagat aatatatca gtatataaa 
      ttgttacct ataattatg ttaatccaa aacaatttt 
      atcttactc attcataga ttcttacac tatcctact 
      ctaacttgt attgtacat gaactgcaa cactggttg 
      atttctttt ggttatctt tagttgcgt aaacatatc 
      gacttcagc gcaccatat aataatgtt tacaatttt 
      ctataaaat agatatttt tccagttat ttagacgta 
      gcaattaaa aatttactc acattccgt aaagtgttg 
      cagtttttc tttcatgga actactttc ctggatgta 
      cctatctac agaaagggt ctttaaata tatcggtca 
      gaaaaatga aagttacta agtaataaa ctacaatac 
      atatttaag taaagacaa tatactcaa ttaatgaaa 
      tgtaaaagc acagccggt atgtgttaa ggattctgg 
      tcaatattt tatatttgt cactgaaac tgtttgtgg 
      atatttgta aaacggagg taatctacc tatgtgtaa 
      cagaaataa atggaacaa tatgtt