Status
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Gene information
General information
TC000749 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 6253397..6274100
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000749
    iBeetle screen iB_00167

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality late (L7) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000749 RA
      MDQIPPPKE VKILETAED IQERRQQVL SRYDNFKAD 
      VRAKREKLE DSRRFQYFK RDADELESW ILEKLQAAS 
      DESYKDPTN LQAKIQKHQ AFEAEVAAH SNAIVVLDN 
      TGREMINQN HYESETIRR RLEELHRLW EQLLSKLAE 
      KGMKLQQAL VLVQFIRQC DEVMFWIND KSTFLTTEE 
      FGHDLEHVE VLQRKFDEF QKDMASQEH RVTQVNELA 
      DKLLQDGHP ERDTIINRK EELNNAWQR LKQMTLMRQ 
      DKLYGAHEI QRFNRDADE TVAWIAEKD VVLSSDDYG 
      RDLASVQAL QRKHEGVER DLAALEDKV STIGKEADR 
      LCAIHGDHA DQIQSKRAE IEDYWQSLT AKAKERRDK 
      LLESYALHR FLSDFRDLV SWINDMKAI ISADELAKD 
      VAGAEALLE RHQEHRGEI DAREDSFAA TTEAGRLLL 
      ENGHYASDE VKEKLSTLV SDKNSLLAL WEERRILYE 
      QCMDLQLFY RDTEQADTW MAKQEAFLA NEDLGDSLD 
      SVEALIKKH EDFEKSLAA QEEKIKALD EFATKLIDG 
      EHYAADDVA QRRAMLLER RSALKEKSA QRRALLEDA 
      YKLQQFERD CDETKGWIN EKLKFATDE SYLDPTNLG 
      GKVQKHQNF EQELNANKT RMEDITSTG QELVDAKHY 
      AAPRIQSRM EEIVNLWET LVQSTDKKS SKLQEASQQ 
      QQFNRTIED VELWLSEIE GQLMSEDYG KDLTSVQNL 
      QKKHALLEA DVASHQDRI EGITAAANQ FVERGHFDA 
      DNIAHKQKV LTDRYTALQ TPMAIRKQR LLDSLQVQQ 
      LFRDIEDEE AWIREKEPI AAGTTRGRD LIGVQNLIK 
      KHQAVLAEI NNHDARISA VVEAGRQMM EDEHFASDQ 
      IRNRVTALN DHWVQLKEK ANQRKQDLD DSLQAHQYY 
      ADANEAESW MKEKEPIVS NTDYGKDED SSEALLKKH 
      EALMSDLIA FGNTIEGLK EQARNCRQQ EPPVVDVTG 
      RETVQALYD YTEKSAREV SMKKGDLLH LLNSSNKDW 
      WKVEIHDRQ GFVPAAYVK KVDAGLTAS QQNLVDNNS 
      ISARQNQIN TQYDRLLAL ARERQNKLN ETVKAYVLV 
      REAAELANW IKDKEMHAQ VQDVGEDLE QVEVMQKKF 
      DDFQTDLKA NEVRLAEMN EIAMQLVSL GQTEAALKI 
      QTQMEDLNT KWTSLQQLT AERANQLGS AHEVQRFHR 
      DVNETIDWI QEKDEALNN DDLGKDLRS VQALQRKHE 
      GLERDLAAL GDKIKQLDE IAGRLVQTH PESAEQTRA 
      KQEEINELW TQLTAKANN RKEKLLDSY DLQRFLNDH 
      RDLMAWMNS MLGLVTSTE LASDVTGSE ALIERHQEH 
      RTEIDARAG TFNALEQFG QQLLSSQHY ASPEIQEKL 
      EQLNEFRKE LETRWIERR VQLDQNLDL NLFYRDCEQ 
      AENWMSDRE AFLASDEVD SNTDNVEAL IKKHEDFDK 
      AINAHEEKI AALESLANQ LIQNEHYAS QDIDNKRKQ 
      VLDRWHHLK EALIEKRSK LGESQTLQQ FSRDADEIE 
      NWIAEKLQL ATEESYKDP ANIQSKHQK HQAFEAELA 
      ANADRIQSV LSNGTNLIE KRQCAGSEE AVQKRLESI 
      ADQWEFLTQ KTTEKSMKL KEANKQRTY IAAVKDLDF 
      WLGEVESLL TSEDAGKDL ASVQNLMKK HQLVEADIQ 
      AHEDRIKDM NDQADSLIE SGQFDTASI QEKRQSINE 
      RYERIKNLA AHRQARLNE ANTLHQFFR DIADEESWI 
      KEKKLLVGS DDYGRDLTG VQNLKKKHK RLEAELGSH 
      EPAIQAVQE AGEKLMDVS NLGVPEIEQ RLKALNQAW 
      AELKQLAAT RGQKLDESL TYQQFLAKV EEEEAWISE 
      KQQLLSVED YGDTMAAVQ GLLKKHDAF ETDFQAHRE 
      RCKDINDAG KKLVAEGNH HADSINQRC QQLQTKLDH 
      LAALAGRRK AKLIDNSAY LQFMWKADV VESWIADKE 
      THVKSEEYG RDLSSVQTL LTKQETFDA GLTAFEHEG 
      IQNITALKD QLIASNHDQ SPAILQRHA DVIDRWQKL 
      LADSDARKQ RLLHMQDQF RQIEDLFLT FAKRASAFN 
      SWFENAEED LTDPVRCNS IEEIRALRE AHAQFQASL 
      SSAQADFEA LAALDQQIK SFNVGPNPY TWFNMEALE 
      ETWRNLQKI IAERDVELN KEAQRQEEN DKLRKEFAK 
      HANAFHQWL TETRTSMME GSGSLEQQL EATKRKAAE 
      VRARRSDLK KIEDLGAIL EEHLILDNR YTEHSTVGL 
      AQQWDQLDQ LGMRMQHNL EQQIQARNQ SGVSEDALK 
      EFSMMFKHF DKEKSGKLN HQEFKSCLR ALGYDLPMV 
      EEGQPDPEF DAILDVVDP NRDGHVSLQ EYMAFMISK 
      ETENVQSSE EIEKAFRAI TAGDRPYVT QEELYANLT 
      KEMADYCVA RMKPYVEPK TERTIAGAL DYIEFTRTL 
      FQN
    CDS sequence
    • >TC000749 RA
      atggatcag atacctccg cccaaagaa gtgaaaatt 
      ctcgaaaca gccgaagac atccaagag cgtcgtcag 
      caggtttta tcccgctat gacaacttc aaagctgac 
      gttcgtgca aaaagagaa aaactcgaa gactcgcgt 
      cgtttccaa tacttcaaa cgcgacgct gacgaactt 
      gaatcatgg atccttgaa aaattgcaa gcagcatcc 
      gacgaaagt tacaaagac ccaactaac ctacaagcc 
      aaaatccaa aaacaccaa gcttttgag gccgaagtt 
      gccgctcat agcaatgca atcgtggtg ttagacaac 
      acaggccgc gaaatgatc aatcaaaat cactacgaa 
      tcggaaacg attcgtcgc agacttgag gaactccac 
      cgactttgg gagcaactc ctttcaaag ctcgccgaa 
      aaaggaatg aagttgcaa caagccctc gtcttggtg 
      cagttcatc cgccagtgt gacgaagtc atgttttgg 
      atcaacgac aaaagcact ttcctcacc accgaggag 
      ttcgggcac gacctggag cacgttgaa gtcctccag 
      cgaaaattc gacgagttc cagaaagac atggcttcg 
      caagagcac cgtgttacc caagtcaac gaactcgct 
      gataaacta cttcaggat ggtcacccg gaacgagac 
      accatcatc aaccgtaaa gaggagctc aacaacgca 
      tggcaacgc ctcaaacag atgactttg atgcgccaa 
      gacaagctt tacggtgct cacgaaatc caaagattt 
      aaccgagat gctgacgaa accgtcgct tggatcgct 
      gaaaaagac gtcgttttg tcgtctgat gactacggg 
      cgtgatcta gccagcgtt caagcgctt caaaggaaa 
      cacgaagga gtggagaga gatttggcg gctcttgaa 
      gacaaagtt tcgacaatt gggaaggaa gccgatcga 
      ttgtgtgcc attcatggc gatcatgcc gatcagatt 
      cagtcgaaa agagccgaa attgaggac tattggcaa 
      agcttgacc gctaaagct aaagaacgc agagataaa 
      ttgttggag tcgtatgct ttgcatcga tttttgtcc 
      gattttaga gacttggtg tcgtggatt aatgacatg 
      aaggcgatt atttcggcc gatgagctg gccaaggat 
      gtggctgga gctgaggct cttttggag aggcatcag 
      gaacacagg ggggagatt gatgcacga gaggatagt 
      tttgcggcg accactgaa gccggaagg ttgttgttg 
      gaaaatgga cattatgcg agcgatgag gttaaggag 
      aagttgtcc acgttggtg tccgacaaa aattctctc 
      ttggctctt tgggaagaa cgccgcatt ctatacgaa 
      caatgtatg gacttgcaa ctattctac cgcgacacc 
      gaacaagcc gacacctgg atggccaaa caagaggcc 
      ttcttagcc aatgaagat ttgggcgat tccctcgac 
      tccgtcgaa gcccttata aaaaaacac gaagatttc 
      gaaaaatcc cttgccgca caagaagag aaaatcaaa 
      gctcttgac gaattcgcg accaaatta atcgatggc 
      gaacattac gctgccgat gatgtcgcc caaagacga 
      gcaatgctc ttagaacgc agatcagca cttaaagaa 
      aaatcagcc caaagacgc gctctcctg gaggatgct 
      tacaaattg caacaattt gaacgcgat tgtgacgaa 
      accaaaggg tggatcaac gaaaaactt aaatttgcg 
      accgatgag agttaccta gatccgact aatttgggc 
      ggaaaagtg cagaaacac cagaatttt gaacaagag 
      cttaatgct aataaaaca cgaatggag gatattacg 
      tcgactggg caggagttg gttgatgct aaacattac 
      gcagctccg aggattcaa tcacgaatg gaggaaatc 
      gtcaacttg tgggagact ttagtgcag tcaactgac 
      aagaagagt tcgaaattg caagaagca agtcaacaa 
      caacaattc aatcggacg attgaagac gttgagttg 
      tggttgtct gagattgaa ggacaactt atgtccgag 
      gattatggc aaggatcta acttccgtc caaaacctt 
      cagaagaaa catgcgtta cttgaggcc gatgttgcg 
      tcccatcaa gaccgtatt gaaggaatc acagctgcg 
      gctaaccaa tttgttgaa cgtggtcac ttcgatgct 
      gataacatc gcccacaaa cagaaggtt ttgactgat 
      cgatacaca gccttgcag acacccatg gctatcaga 
      aagcaacgt cttttggac tccttgcaa gtgcaacag 
      ttattcaga gacatcgaa gatgaggag gcttggatt 
      cgcgaaaaa gagccaata gctgcaggg acaacacga 
      ggacgtgat ttgatcggg gttcagaat ttgatcaag 
      aaacatcaa gccgttctg gccgaaatt aacaatcac 
      gatgctcgc attagtgcc gttgttgag gccggtaga 
      caaatgatg gaagatgag cactttgcc agtgaccaa 
      attcgcaac agagtgacg gctctcaat gatcattgg 
      gtccagttg aaggagaag gccaaccag aggaaacaa 
      gatctggat gattcgctt caagcccat cagtactat 
      gctgatgca aacgaggct gaatcctgg atgaaggag 
      aaagaacca attgtgagc aatactgac tacggtaaa 
      gatgaggat tcgtccgag gctttgttg aagaaacat 
      gaggctttg atgagtgat ttgatcgcg tttggaaat 
      acgattgaa ggtttgaag gaacaagcg cgcaattgt 
      agacaacag gaaccacca gttgttgat gttactgga 
      cgggaaacc gttcaagct ttgtacgat tatacagag 
      aagtcggct cgcgaagtt tcgatgaag aagggcgat 
      ttgttgcat cttttgaat tccagtaat aaggattgg 
      tggaaagtg gaaatccac gaccgccaa ggctttgtc 
      cctgccgct tacgtgaaa aaagtggat gctggactc 
      acagcctca caacaaaat ctcgttgat aacaactcc 
      atctctgcg cgacaaaac caaatcaac acccaatat 
      gaccgtctc cttgctctt gctcgtgag cgtcaaaac 
      aaactcaat gaaacggtc aaggcttat gttctggtc 
      cgcgaagcg gccgaactt gcaaactgg atcaaagac 
      aaggaaatg cacgctcaa gtccaagac gttggtgaa 
      gatctggaa caagtggaa gtgatgcag aagaaattc 
      gatgacttc cagacagat ttgaaagcc aacgaagtg 
      cgtttggct gaaatgaat gaaattgca atgcagttg 
      gtgtctttg gggcaaact gaagctgcg cttaagatc 
      cagacacag atggaggat ttgaacacg aaatggacg 
      tctttgcag cagttgacg gcagagagg gcgaaccag 
      ttggggtcg gcacatgaa gttcagagg ttccacaga 
      gatgtgaat gagacgatt gattggatt caggagaaa 
      gacgaagct ttgaataat gatgatttg gggaaggat 
      ttgaggagt gttcaagcc ttgcagagg aaacatgag 
      gggttggag agagatttg gccgcttta ggggacaag 
      attaagcaa cttgatgag atcgccggt cgattggtc 
      caaactcat ccggaaagt gccgaacag actcgagct 
      aaacaagag gaaatcaac gaattgtgg actcaattg 
      accgccaaa gccaacaat cgtaaagaa aaactgctc 
      gattcgtac gatttgcaa cgttttctc aatgatcat 
      cgcgacttg atggcttgg atgaattct atgcttggt 
      ttggttacg tctacggag ttggctagt gatgtgacg 
      gggtcagag gcgctgatt gaacgtcat caggaacat 
      cgaactgaa atcgatgca cgtgcggga actttcaac 
      gcacttgaa caattcgga caacaactc ctcagttcc 
      caacattat gcaagcccc gaaattcaa gaaaaactc 
      gaacaactc aacgaattc cgcaaggaa ttagagacg 
      cgttggatc gaacgacga gtccagttg gaccaaaat 
      ctcgacttg aacctcttc tacagggac tgcgagcaa 
      gcagaaaac tggatgtcc gatcgagag gcgttcctt 
      gcgtcagac gaagtcgac tccaacacc gataacgtc 
      gaggctctg atcaagaaa cacgaagat tttgataaa 
      gcgattaat gcgcatgag gaaaaaatc gccgcactt 
      gaaagtttg gccaaccag ctcatccag aacgaacac 
      tacgcttcc caagacatt gacaacaag cgcaagcaa 
      gtgttggat cgttggcac cacctcaaa gaggccctc 
      atcgaaaaa cgctcaaaa cttggcgaa agccaaacc 
      ttgcagcaa ttctcacgc gatgctgac gaaatcgag 
      aactggatc gctgaaaag ttacaactg gcgaccgaa 
      gagagctac aaagacccg gccaacatt cagtcgaag 
      catcagaaa caccaagct ttcgaggcc gaattggcc 
      gcaaacgcc gacagaatt cagtcggta ttgtcgaac 
      ggtacgaat ttgattgag aaacgacaa tgtgccggg 
      tcggaagaa gctgttcag aaacggctc gagtccatc 
      gctgatcag tgggagttt ttgacgcaa aaaaccacc 
      gagaaatcg atgaaattg aaggaggcc aacaagcaa 
      aggacttac attgcggcc gttaaggat ttggacttc 
      tggttgggc gaagttgaa agtttgttg acgagtgaa 
      gatgctggg aaagattta gcatcggtg cagaattta 
      atgaagaag catcagttg gtcgaggct gatattcag 
      gcccatgag gacagaatt aaagatatg aatgatcaa 
      gctgattct cttattgaa agtggacaa tttgacacg 
      gcttcaatt caagaaaaa cgacaatcg attaatgag 
      cgttacgaa cgtattaag aatttggcc gctcatcga 
      caagctcgg ctcaatgaa gctaatacg ttgcatcag 
      ttcttccgc gatattgct gatgaagaa tcctggatc 
      aaggagaag aaacttttg gtgggttcg gacgactac 
      ggtcgtgac ttgaccggc gtccaaaac ttgaaaaag 
      aaacacaaa cgtctcgaa gccgaatta ggctcacac 
      gaaccggcc attcaagcg gttcaagaa gctggcgaa 
      aaactaatg gacgtatca aaccttggt gtccccgaa 
      atcgagcaa cgtctcaaa gctttgaac caagcttgg 
      gccgaattg aaacaactg gccgccact cgtggccaa 
      aaactggac gagtcattg acttaccaa caattcctg 
      gctaaggtc gaagaagag gaagcttgg attagtgaa 
      aaacaacaa ttgttgagt gttgaagat tatggtgat 
      accatggct gcggttcaa ggtttattg aagaaacat 
      gacgctttt gaaaccgat ttccaagca cacagagag 
      aggtgcaag gatattaac gatgctggg aagaaattg 
      gttgcggaa gggaaccat catgctgat agtattaat 
      cagcgttgt caacaattg caaactaag ttagaccat 
      ttggcggct ttggctggc cgccgcaag gccaaattg 
      atcgataat tcggcctat ttgcaattt atgtggaag 
      gcggatgtt gtggaaagt tggatcgct gataaggaa 
      acgcatgtc aagagtgag gagtatggc agggatttg 
      tcgtcggtg cagacgttg ctcacgaaa caggagaca 
      tttgatgca ggtctcact gcgtttgag cacgagggc 
      atccagaac atcaccgcc ctaaaggac caactgatc 
      gcttcaaat cacgaccaa agtccggcg attttacaa 
      cgtcacgcc gatgtgatc gacagatgg cagaaacta 
      ttggccgac tctgacgcc cgcaaacag cgtcttctc 
      cacatgcaa gaccaattc cgacaaatc gaagatctt 
      ttcctcact tttgccaag agagcgtct gcgttcaat 
      tcttggttt gaaaatgct gaggaggat ttgaccgat 
      ccagtgcga tgcaattcc attgaggag atccgagcg 
      ttgagagag gctcatgca caattccag gcatctcta 
      tcgagcgcg caagccgat tttgaggct ttggcagct 
      ttggatcaa cagatcaag agtttcaat gttggaccg 
      aatccctac acttggttt aatatggaa gctttggaa 
      gaaacgtgg aggaacttg cagaaaatc atcgccgag 
      agggacgta gagttgaat aaagaggcc caaaggcag 
      gaagaaaat gataagttg aggaaggag tttgctaag 
      catgccaat gccttccat caatggttg accgaaact 
      cgtacctca atgatggaa ggctccggc tcattggag 
      cagcagctg gaagccacc aaacgcaaa gcagccgaa 
      gtgcgcgcc cgtcgctcc gacttgaag aaaatcgaa 
      gatttggga gcaattctt gaagaacat ctcatcctg 
      gacaaccgc tacactgag cattctact gttggttta 
      gcccaacag tgggatcaa ttagaccaa ttgggcatg 
      agaatgcag cataatctt gaacaacag atacaagct 
      cgcaaccaa tcaggtgtg agtgaggac gctcttaag 
      gagttctcg atgatgttc aaacacttt gacaaggaa 
      aaatccgga aaattgaac catcaagaa ttcaagagt 
      tgtttgaga gctttgggt tacgatctg ccgatggtg 
      gaggaaggc caacctgat ccagaattc gatgcgatt 
      ttggatgtg gtggatccg aatagagat gggcacgtg 
      tcgttgcag gagtacatg gcgtttatg atcagtaaa 
      gagaccgaa aatgtgcaa agttcggag gaaattgag 
      aaggcgttc agggctatc acagcaggg gatagacca 
      tatgttacg caagaggag ctatatgct aatttgacg 
      aaggaaatg gccgattat tgcgtagct cgtatgaaa 
      ccctacgtt gaacccaag accgaacgc acaatcgca 
      ggggccctg gattacata gagtttact cgtactctc 
      ttccagaat taa
    mRNA sequence
    • >TC000749 RA
      actgacgtc atttccagt aatagttgt gtcagtgag 
      tgccatgtt taatgtcaa gtaaagttt ttggataaa 
      aactgaatt ttgtgtact gttttaact tcaagagga 
      ttatattat cgtcaaaaa agtcacagg caagaaaac 
      cgattcgcg atggatcag atacctccg cccaaagaa 
      gtgaaaatt ctcgaaaca gccgaagac atccaagag 
      cgtcgtcag caggtttta tcccgctat gacaacttc 
      aaagctgac gttcgtgca aaaagagaa aaactcgaa 
      gactcgcgt cgtttccaa tacttcaaa cgcgacgct 
      gacgaactt gaatcatgg atccttgaa aaattgcaa 
      gcagcatcc gacgaaagt tacaaagac ccaactaac 
      ctacaagcc aaaatccaa aaacaccaa gcttttgag 
      gccgaagtt gccgctcat agcaatgca atcgtggtg 
      ttagacaac acaggccgc gaaatgatc aatcaaaat 
      cactacgaa tcggaaacg attcgtcgc agacttgag 
      gaactccac cgactttgg gagcaactc ctttcaaag 
      ctcgccgaa aaaggaatg aagttgcaa caagccctc 
      gtcttggtg cagttcatc cgccagtgt gacgaagtc 
      atgttttgg atcaacgac aaaagcact ttcctcacc 
      accgaggag ttcgggcac gacctggag cacgttgaa 
      gtcctccag cgaaaattc gacgagttc cagaaagac 
      atggcttcg caagagcac cgtgttacc caagtcaac 
      gaactcgct gataaacta cttcaggat ggtcacccg 
      gaacgagac accatcatc aaccgtaaa gaggagctc 
      aacaacgca tggcaacgc ctcaaacag atgactttg 
      atgcgccaa gacaagctt tacggtgct cacgaaatc 
      caaagattt aaccgagat gctgacgaa accgtcgct 
      tggatcgct gaaaaagac gtcgttttg tcgtctgat 
      gactacggg cgtgatcta gccagcgtt caagcgctt 
      caaaggaaa cacgaagga gtggagaga gatttggcg 
      gctcttgaa gacaaagtt tcgacaatt gggaaggaa 
      gccgatcga ttgtgtgcc attcatggc gatcatgcc 
      gatcagatt cagtcgaaa agagccgaa attgaggac 
      tattggcaa agcttgacc gctaaagct aaagaacgc 
      agagataaa ttgttggag tcgtatgct ttgcatcga 
      tttttgtcc gattttaga gacttggtg tcgtggatt 
      aatgacatg aaggcgatt atttcggcc gatgagctg 
      gccaaggat gtggctgga gctgaggct cttttggag 
      aggcatcag gaacacagg ggggagatt gatgcacga 
      gaggatagt tttgcggcg accactgaa gccggaagg 
      ttgttgttg gaaaatgga cattatgcg agcgatgag 
      gttaaggag aagttgtcc acgttggtg tccgacaaa 
      aattctctc ttggctctt tgggaagaa cgccgcatt 
      ctatacgaa caatgtatg gacttgcaa ctattctac 
      cgcgacacc gaacaagcc gacacctgg atggccaaa 
      caagaggcc ttcttagcc aatgaagat ttgggcgat 
      tccctcgac tccgtcgaa gcccttata aaaaaacac 
      gaagatttc gaaaaatcc cttgccgca caagaagag 
      aaaatcaaa gctcttgac gaattcgcg accaaatta 
      atcgatggc gaacattac gctgccgat gatgtcgcc 
      caaagacga gcaatgctc ttagaacgc agatcagca 
      cttaaagaa aaatcagcc caaagacgc gctctcctg 
      gaggatgct tacaaattg caacaattt gaacgcgat 
      tgtgacgaa accaaaggg tggatcaac gaaaaactt 
      aaatttgcg accgatgag agttaccta gatccgact 
      aatttgggc ggaaaagtg cagaaacac cagaatttt 
      gaacaagag cttaatgct aataaaaca cgaatggag 
      gatattacg tcgactggg caggagttg gttgatgct 
      aaacattac gcagctccg aggattcaa tcacgaatg 
      gaggaaatc gtcaacttg tgggagact ttagtgcag 
      tcaactgac aagaagagt tcgaaattg caagaagca 
      agtcaacaa caacaattc aatcggacg attgaagac 
      gttgagttg tggttgtct gagattgaa ggacaactt 
      atgtccgag gattatggc aaggatcta acttccgtc 
      caaaacctt cagaagaaa catgcgtta cttgaggcc 
      gatgttgcg tcccatcaa gaccgtatt gaaggaatc 
      acagctgcg gctaaccaa tttgttgaa cgtggtcac 
      ttcgatgct gataacatc gcccacaaa cagaaggtt 
      ttgactgat cgatacaca gccttgcag acacccatg 
      gctatcaga aagcaacgt cttttggac tccttgcaa 
      gtgcaacag ttattcaga gacatcgaa gatgaggag 
      gcttggatt cgcgaaaaa gagccaata gctgcaggg 
      acaacacga ggacgtgat ttgatcggg gttcagaat 
      ttgatcaag aaacatcaa gccgttctg gccgaaatt 
      aacaatcac gatgctcgc attagtgcc gttgttgag 
      gccggtaga caaatgatg gaagatgag cactttgcc 
      agtgaccaa attcgcaac agagtgacg gctctcaat 
      gatcattgg gtccagttg aaggagaag gccaaccag 
      aggaaacaa gatctggat gattcgctt caagcccat 
      cagtactat gctgatgca aacgaggct gaatcctgg 
      atgaaggag aaagaacca attgtgagc aatactgac 
      tacggtaaa gatgaggat tcgtccgag gctttgttg 
      aagaaacat gaggctttg atgagtgat ttgatcgcg 
      tttggaaat acgattgaa ggtttgaag gaacaagcg 
      cgcaattgt agacaacag gaaccacca gttgttgat 
      gttactgga cgggaaacc gttcaagct ttgtacgat 
      tatacagag aagtcggct cgcgaagtt tcgatgaag 
      aagggcgat ttgttgcat cttttgaat tccagtaat 
      aaggattgg tggaaagtg gaaatccac gaccgccaa 
      ggctttgtc cctgccgct tacgtgaaa aaagtggat 
      gctggactc acagcctca caacaaaat ctcgttgat 
      aacaactcc atctctgcg cgacaaaac caaatcaac 
      acccaatat gaccgtctc cttgctctt gctcgtgag 
      cgtcaaaac aaactcaat gaaacggtc aaggcttat 
      gttctggtc cgcgaagcg gccgaactt gcaaactgg 
      atcaaagac aaggaaatg cacgctcaa gtccaagac 
      gttggtgaa gatctggaa caagtggaa gtgatgcag 
      aagaaattc gatgacttc cagacagat ttgaaagcc 
      aacgaagtg cgtttggct gaaatgaat gaaattgca 
      atgcagttg gtgtctttg gggcaaact gaagctgcg 
      cttaagatc cagacacag atggaggat ttgaacacg 
      aaatggacg tctttgcag cagttgacg gcagagagg 
      gcgaaccag ttggggtcg gcacatgaa gttcagagg 
      ttccacaga gatgtgaat gagacgatt gattggatt 
      caggagaaa gacgaagct ttgaataat gatgatttg 
      gggaaggat ttgaggagt gttcaagcc ttgcagagg 
      aaacatgag gggttggag agagatttg gccgcttta 
      ggggacaag attaagcaa cttgatgag atcgccggt 
      cgattggtc caaactcat ccggaaagt gccgaacag 
      actcgagct aaacaagag gaaatcaac gaattgtgg 
      actcaattg accgccaaa gccaacaat cgtaaagaa 
      aaactgctc gattcgtac gatttgcaa cgttttctc 
      aatgatcat cgcgacttg atggcttgg atgaattct 
      atgcttggt ttggttacg tctacggag ttggctagt 
      gatgtgacg gggtcagag gcgctgatt gaacgtcat 
      caggaacat cgaactgaa atcgatgca cgtgcggga 
      actttcaac gcacttgaa caattcgga caacaactc 
      ctcagttcc caacattat gcaagcccc gaaattcaa 
      gaaaaactc gaacaactc aacgaattc cgcaaggaa 
      ttagagacg cgttggatc gaacgacga gtccagttg 
      gaccaaaat ctcgacttg aacctcttc tacagggac 
      tgcgagcaa gcagaaaac tggatgtcc gatcgagag 
      gcgttcctt gcgtcagac gaagtcgac tccaacacc 
      gataacgtc gaggctctg atcaagaaa cacgaagat 
      tttgataaa gcgattaat gcgcatgag gaaaaaatc 
      gccgcactt gaaagtttg gccaaccag ctcatccag 
      aacgaacac tacgcttcc caagacatt gacaacaag 
      cgcaagcaa gtgttggat cgttggcac cacctcaaa 
      gaggccctc atcgaaaaa cgctcaaaa cttggcgaa 
      agccaaacc ttgcagcaa ttctcacgc gatgctgac 
      gaaatcgag aactggatc gctgaaaag ttacaactg 
      gcgaccgaa gagagctac aaagacccg gccaacatt 
      cagtcgaag catcagaaa caccaagct ttcgaggcc 
      gaattggcc gcaaacgcc gacagaatt cagtcggta 
      ttgtcgaac ggtacgaat ttgattgag aaacgacaa 
      tgtgccggg tcggaagaa gctgttcag aaacggctc 
      gagtccatc gctgatcag tgggagttt ttgacgcaa 
      aaaaccacc gagaaatcg atgaaattg aaggaggcc 
      aacaagcaa aggacttac attgcggcc gttaaggat 
      ttggacttc tggttgggc gaagttgaa agtttgttg 
      acgagtgaa gatgctggg aaagattta gcatcggtg 
      cagaattta atgaagaag catcagttg gtcgaggct 
      gatattcag gcccatgag gacagaatt aaagatatg 
      aatgatcaa gctgattct cttattgaa agtggacaa 
      tttgacacg gcttcaatt caagaaaaa cgacaatcg 
      attaatgag cgttacgaa cgtattaag aatttggcc 
      gctcatcga caagctcgg ctcaatgaa gctaatacg 
      ttgcatcag ttcttccgc gatattgct gatgaagaa 
      tcctggatc aaggagaag aaacttttg gtgggttcg 
      gacgactac ggtcgtgac ttgaccggc gtccaaaac 
      ttgaaaaag aaacacaaa cgtctcgaa gccgaatta 
      ggctcacac gaaccggcc attcaagcg gttcaagaa 
      gctggcgaa aaactaatg gacgtatca aaccttggt 
      gtccccgaa atcgagcaa cgtctcaaa gctttgaac 
      caagcttgg gccgaattg aaacaactg gccgccact 
      cgtggccaa aaactggac gagtcattg acttaccaa 
      caattcctg gctaaggtc gaagaagag gaagcttgg 
      attagtgaa aaacaacaa ttgttgagt gttgaagat 
      tatggtgat accatggct gcggttcaa ggtttattg 
      aagaaacat gacgctttt gaaaccgat ttccaagca 
      cacagagag aggtgcaag gatattaac gatgctggg 
      aagaaattg gttgcggaa gggaaccat catgctgat 
      agtattaat cagcgttgt caacaattg caaactaag 
      ttagaccat ttggcggct ttggctggc cgccgcaag 
      gccaaattg atcgataat tcggcctat ttgcaattt 
      atgtggaag gcggatgtt gtggaaagt tggatcgct 
      gataaggaa acgcatgtc aagagtgag gagtatggc 
      agggatttg tcgtcggtg cagacgttg ctcacgaaa 
      caggagaca tttgatgca ggtctcact gcgtttgag 
      cacgagggc atccagaac atcaccgcc ctaaaggac 
      caactgatc gcttcaaat cacgaccaa agtccggcg 
      attttacaa cgtcacgcc gatgtgatc gacagatgg 
      cagaaacta ttggccgac tctgacgcc cgcaaacag 
      cgtcttctc cacatgcaa gaccaattc cgacaaatc 
      gaagatctt ttcctcact tttgccaag agagcgtct 
      gcgttcaat tcttggttt gaaaatgct gaggaggat 
      ttgaccgat ccagtgcga tgcaattcc attgaggag 
      atccgagcg ttgagagag gctcatgca caattccag 
      gcatctcta tcgagcgcg caagccgat tttgaggct 
      ttggcagct ttggatcaa cagatcaag agtttcaat 
      gttggaccg aatccctac acttggttt aatatggaa 
      gctttggaa gaaacgtgg aggaacttg cagaaaatc 
      atcgccgag agggacgta gagttgaat aaagaggcc 
      caaaggcag gaagaaaat gataagttg aggaaggag 
      tttgctaag catgccaat gccttccat caatggttg 
      accgaaact cgtacctca atgatggaa ggctccggc 
      tcattggag cagcagctg gaagccacc aaacgcaaa 
      gcagccgaa gtgcgcgcc cgtcgctcc gacttgaag 
      aaaatcgaa gatttggga gcaattctt gaagaacat 
      ctcatcctg gacaaccgc tacactgag cattctact 
      gttggttta gcccaacag tgggatcaa ttagaccaa 
      ttgggcatg agaatgcag cataatctt gaacaacag 
      atacaagct cgcaaccaa tcaggtgtg agtgaggac 
      gctcttaag gagttctcg atgatgttc aaacacttt 
      gacaaggaa aaatccgga aaattgaac catcaagaa 
      ttcaagagt tgtttgaga gctttgggt tacgatctg 
      ccgatggtg gaggaaggc caacctgat ccagaattc 
      gatgcgatt ttggatgtg gtggatccg aatagagat 
      gggcacgtg tcgttgcag gagtacatg gcgtttatg 
      atcagtaaa gagaccgaa aatgtgcaa agttcggag 
      gaaattgag aaggcgttc agggctatc acagcaggg 
      gatagacca tatgttacg caagaggag ctatatgct 
      aatttgacg aaggaaatg gccgattat tgcgtagct 
      cgtatgaaa ccctacgtt gaacccaag accgaacgc 
      acaatcgca ggggccctg gattacata gagtttact 
      cgtactctc ttccagaat taatacact tgcttgtcg 
      tttaatagg gatagggag ctttgtcac tgcacgatt 
      ttaagcttg attttataa tatgtttgc atgtatcac 
      ctaattaat tatatttat ccttttcaa aatatatgg 
      ct
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